2015 Catalog - Pinnacle Technology, Inc
Contents
1. ee ce eee ene ences cea rtm 7 217 Miti E A Dm 8 Calibration sep EEES 8 CGMS SOM Glucose SSW sees 9 GSS RI acest ts i a cece eae ca ecg saw ive mp 10 COMS PEN 10 EEG EMG CDS IT tice rier aces EE EE S EENE S NAST NEES EAS AEE E AE LEA S EE EE EEA S E EA A A ET 11 13 Three Ghaninel Systems for Mice and smscsacevescecaxctexcasacnenrsanasceciowrssexessennxataceetansachoninenanccenesasdonetiuaxeat easersxone asseeomersiausanseanteacsesaerxnneac lt oranesenoasextentqenesexns 14 Channel Systems Tor Mice and FANS pute da e d 15 EEG EMG Biosensor Systems Tor Mice and dena AED rU 15 Wireless EEG for Rats 16 17 OPTOGENETICS QPTOGENETCS 18 19 ie I el secede cette tgs skeen Em A Lo me 18 LED Fiber Probes etr 19 AN qo OQ 19 FSCV FN 20 21 R2. 9057 Enhanced Illuminator 9057 EN SIRENIA Pinnacle s SIRENIA SOFTWARE provides powerful tools for preclinical research Our FREE Sirenia platform includes acquisition and basic analysis software and users can easily add PREMIUM MODULES at any time KEY FEATURES Supports synchronized video I O functionality Data consolidation Flexible data management Record from multiple Pinnacle devices using one platform Multiple export capabilities FREE ACQUISITION SOFTWARE SLEEP BAS o v Ie O44 1 E COMING SOON FREESOFTWARE Sirenia Acquisition provides a single platform for recording data from any Pinnacle hardware system excluding FSCV The software features synchronization of all data streams user configurable settings data consolidation and multiple export options the download includes basic review and analysis modules for biosensor sleep and seizure recordings Sirenia delivers all in one software that is ideal for data acquisition and review VISIT OUR WEBSITE TO DOWNLOAD SIRENIA TODAY www pinnaclet com sirenia download html PREMIUM MODULES Product Item Sirenia Seizure Pro Analysis Software 9037 Sirenia Sleep Pro Analysis Software 9035 Sirenia Visualization Software 9074 Sirenia X Y Tracking Software 9039 Sirenia CGMS Software 9041 The software packages can be installed on multiple computers though each seat is limited to one compute
3. Feedback for Mice 9000 5 Stand Alone for Rats 9000 K6 S SIMULATED SHIFT WORK AUTOMATED DEPRIVATION l SLEEP FRAGMENTATION Feedback for Rats 9000 K6 STAND ALONE SYSTEM The STAND ALONE SYSTEM provides calendar based functionality for programming the bar to rotate at discrete intervals Programming options range from a second by second basis to hourly daily weekly or monthly intervals Use the device s touchscreen to operate the system without a computer connection The system is compatible with most EEG EMG hardware and physiological measurement systems FEEDBACK SYSTEM The FEEDBACK SYSTEM provides calendar scheduling functionality plus the capability of adding real time EEG EMG feedback to ensure the bar rotates only when the animal enters a sleep like state Bar rotation starts and stops automatically based on user established rule sets for the animal s sleep state and users can easily incorporate delays shifts in bar rotation and time restrictions into the experimental setup ADD SYNCHRONIZED VIDEO TO YOUR SYSTEM SEE PAGE 22 In 2010 Nature Methods declared the Method of the Year The same year Science called it the Breakthrough of the Decade To help our customers Pinnacle is developing a user friendly turn key hardware and software system integrating electrophysiological and neurotransmitter measurements along with video for behavioral studies with precise optogenetic control The system i
4. 8502 Bluetooth dongle 7038 Probe clamp accessory 8507 Rat Hat top 9052 USB extension cable 7039 Allen wrench 8508 Rat Hat bottom 4 Soars and EEE EEE nn 8111 1 8 screws pkg of 12 8509 100K test load 8112 Drill bit 8510 100M test load 8147 A Hex screwdriver 9024 Battery charger Pinnacle s wireless FSCV system 8241 S Screwdriver for 1 8 screws KEY FEATURES SWEEP RATE 5 SWEEPS SECOND BLUETOOTH TRANSMISSION Ag AgCl reference electrodes 1000 POINTS SWEEP RECORD FROM MULTIPLE ANIMALS WITHIN THE SAME ROOM are sold separately Carbon fiber sensors and FOR MICE FOR RATS DISPOSABLE Product Product 7033 headmount 8111 1 8 screws pkg of 12 xs ITE MS 8212 0 12 screws pkg of 8 8112 Drill bit 8254 23 gauge needle 8506 Battery with cover 9033 NiMH batteries pkg of 4 8508 Rat Hat bottom SYNCHRONIZED VIDEO 22 SYNCHRONIZED VIDEO CAPABILITY Pinnacle s VIDEO SYSTEM can record up to four simultaneous in vivo experiments on one computer Our Sirenia software synchronizes video recordings with biopotential and biosensor data providing an accurate representation of an animal s overt behavior in conjunction with its physiological response The video system consists of two components a base computer package and a camera package Together they provide everything you need to incorporate video capture into your research The video system is co
5. PINNACLE NEUROPHYSIOLOGICAL PRODUCTS FOR PRECLINICAL IN VIVORESEARCH PINNACLE TECHNOLOGY INC 2721 OREGON STREET LAWRENCE KS 66046 WWW PINNACLET COM PHONE 785 832 8866 FAX 785 749 9214 SALES PINNACLET COM Thank you for choosing Pinnacle Technology for your research needs We offer a range of turn key systems for neurophysiological studies using freely moving animals and are committed to developing new tools that simplify measurement reduce cost and enable new research In 2 N N A C L addition Pinnacle offers a host of supporting products ranging from cages to software analysis TECHNOLOGY INC suites We pride ourselves in providing exceptional customer service and are available to assist you with every stage of your research process By forging collaborative relationships with our clients we are able to develop cutting edge tools that improve and simplify your research We look forward to working with you All the Best A Donna A Johnson President and CEO INFORMATION AND POLICIES Website www pinnaclet com Phone 785 832 8866 Shipping Address 2721 Oregon Street Lawrence KS 66046 Email sales pinnaclet com Fax 785 749 9214 USA Ordering Information General Products may be ordered directly from Pinnacle Technology Inc or from one of our approved distributors see http www pinnaclet com distributors html Some products may not be available in all countries Biosensors and Carb
6. and second derivative of lactate concentration change black SIRENIA X Y TRACKING SIRENIA X Y TRACKING enables users to accurately detect and analyze animal movement within a cage Users can track locomotor behavior in real time or in previously recorded video data In addition our software can be calibrated to different cage types and is compatible with tethered and wireless animals making Sirenia gt a ee ee Parma jas x X Y tracking applicable to a variety of experimental paradigms Analyzed data can be saved as high resolution images or exported as TSV EDF and TXT files k Ded Od mi LO Above Tethered mouse data were recorded using Pinnacle s box camera and IR illuminator Movement was then analyzed with Sirenia X Y Tracking The animal s motion trajectory an X Y heat map and distance and velocity data are displayed on the right side of the screen Left An X Y heat map is displayed over the video image Settings are fully customizable Real time tracking Motion trajectory analysis User defined regions X Y heat maps KEY FEATURES Distance and velocity traveled Quadrant analysis 9 5 ADDITIONAL PRODUCTS ANIMAL HOUSING ce Cage for mice 10 diameter 8 tall 25 4 x 20 3 cm CIRCULAR CAGES AND CAGE ACCESSORIES are designed to Cage for rats 12 diameter 12 tall 30
7. across an implanted carbon fiber sensor and measuring the resultant current Both DEPRESSION LEARNING the tethered system for mice and the wireless system for rats have built in support for controlling an external stimulus The system is shipped with Pinnacle s 8500 WELL BEING SLEEP software EXCELLENT TEMPORAL RESOLUTION SWEEP SPECS SYNCHRONIZED TURN KEY Voltage span 1 1 V to 41 3 V VIDEO SYSTEM Range 250 400 V s oweeps second 5 10 ADVANTAGES Points sweep 1000 GOOD SPATIAL FREE RESOLUTION v 4 SOFTWARE BUILT IN STIMULUS Oy SUPPORT tio Pinnacle offers a complete line of carbon fiber sensors that have been optimized for FSCV applications SEE PAGES 4 5 FOR MORE INFORMATION SOFTWARE FEATURES The 8500 software supports traditional short recording paradigms recordings of two minutes or less as well as longer term recordings using an extended continuous mode Background subtraction Heat maps 3D visualization User selectable filters Animated voltammograms Data export A screenshot of Pinnacle s 8500 software with integrated video TETHERED SYSTEM FOR MICE sss The TETHERED FSCV SYSTEM allows researchers to harness the powerful genetics of the mouse model to address underlying mechanisms of biogenic neurotransmitter release and function A head mounted FSCV board sends signals through a low torque commutator to an interface b
8. 10 z 5 5 o 0 5 0 30 60 90 120 150 180 210 240 Time min 7000 K2 W P CORRELATING CURRENT TO CONCENTRATION Shown in the image on the left are in vivo recordings from two alcohol biosensors implanted contralaterally in the cortex of a Wistar rat An ethanol bolus 1 g kg was delivered at the sixty minute mark Data were transformed to changes in ethanol concentration based on each sensor s post calibration INSET The raw untransformed current nA from the two alcohol biosensors as acquired through Pinnacle s acquisition software CGMS CONTINUOUS GLUCOSE MONITORING Pinnacle s CONTINUOUS GLUCOSE MONITORING SYSTEM CGMS is designed to obtain real time interstitial glucose measurements in freely moving rats with one second temporal resolution Our turn key system capitalizes on over 15 years of experience as innovators in rodent biosensor technology to deliver a simple 17 day subcutaneous sensor for use in diabetes and metabolic studies The sensor is connected to a backpack wireless Bluetooth transmitter Combine this with our user configurable CGMS software for remote monitoring and easy data analysis HARDWARE A durable tear resistant water resistant pouch houses the electronics and battery low power wireless potentiostat applies a bias and transmits up to two digitized signals to a Bluetooth USB dongle that interfaces with Pinnacle s Sirenia Acquisition software for data rec
9. A combination of cluster threshold and manual scoring tools were used by four experienced and novice scorers to separately score three different mouse data files All files were compared to expert hand scored data files The overall average agreement of the four scorers for all the files as compared to the expert is shown below Sleep State Wake 89 7 NREM 94 7 REM 80 2 0 20 40 60 80 100 Percentage Correct Compared to Expert i CPP HAENEN Automated seizure identification Power analysis Spectral density heat maps Time analysis Spectral plots Seizure statistics ACCURACY OF SEIZURE DETECTION Seizure detection was performed using RMS power and line length separately on five individual mouse data files All files were compared to an expert hand scorer s files Agreement of the two detection methods as compared to the expert is shown below of Seizure Accuracy vs of False Positiv Events Marked Expert Scorer URE eds A N 100 100 Data courtesy of Drs Philip Haydon and Jerome Clasadonte Expert Scorer Tufts University School of Medicine Department of Neuroscience Expert Scorer RMS Power Line Length VISUALIZATION SOFTWARE INCLUDED SEE PAGE 25 FOR DETAILS SOFTWARE SIRENIA VISUALIZATION SIRENIA VISUALIZATION allows for fast and easy manipulation and transformation of time series data Data can be easily transformed using a single constant applied to the
10. Return Merchandise Authorization RMA number Contact a Pinnacle Technology Inc representative to obtain an RMA number and proper RMA documentation Returns should be shipped to Pinnacle Technology Inc within 30 days of RMA number issuance RMA documentation must be included in the return shipment and the customer is responsible for all shipping and handling charges Standard items that have not been used or damaged may be returned within 10 days of original delivery for a credit or refund A 1596 restocking charge will be deducted from the refund or credit at Pinnacle Technology Inc s discretion Pinnacle Technology Inc does not offer refunds or credits on special custom or made to order products with custom modifications All products returned for repair or replacement must be sanitary cleaned appropriately and securely packaged Warranty Information In general products are warranted against defects in material and workmanship Purchasers must comply with Pinnacle Technology Inc s policy regarding returns Refer to Pinnacle Technology Inc s website www pinnaclet com for detailed warranty information TABLE OF CONTENTS 8 Biosensors and Carbon Fiber Sensors EE om 4 AUS SANS tine asics ce teams tea tic tele pata 5 Wireless iS E A POE A A 6 TOF MIOC rca
11. dark cycle Components of this kit are the same as above except 8165 S is replaced with 8176 and 9080 is replaced with 9065 8164 M 10 SENSORS Pinnacles CONTINUOUS MONITORING SUBCUTANEOUS SENSORS are designed for easy implantation allowing for a rapid and simple surgery The sensors allow for raw second by second data collection for real time data analysis For more details on the sensor see page 4 SOFTWARE Pinnacle s SIRENIA CGMS SOFTWARE provides a turn key solution CGMS CGMS SENSOR SPECIFICATIONS Sensor Range 10 500 mg dL Sensor Life 17 days Our CGMS glucose sensors do not react to the following interferents galactose creatinine urate xylose warfarin acetaminophen naproxen maltose xanthine and aspirin BIOSENSOR 7006 Glucose Subcutaneous Biosensor Glucose KEY FEATURES for acquiring monitoring and analyzing preclinical glucose data Our software s versatile tools enable users to quickly visualize trends monitor Daily Modal Alerts Programmable Alerts data acquisition remotely calculate comprehensive statistical reports Easy Trend Visualization Remote Monitoring and create customized charts and graphs Data statistics and graphs can be exported as high resolution images for professional presentations Pinnacle s software is specifically tailored for CGMS experiments saving you time by simplifying your data analysis SOFTWARE 9041 K Sirenia CGMS Softw
12. en one Learn more about using 8112 Drill bit preamplifiers with third party systems 5 ITE MS 8239 2 EEG 1 EMG rat headmount on page 27 8239 SE3 3 EEG rat headmount 8247 1 8 screws with wire leads 8425 2 pin electrode EEG EMG SYSTEMS FOUR CHANNEL SYSTEMS WITH BIOSENSOR OPTION The FOUR CHANNEL TETHERED SYSTEM supports up to four biopotential input channels This system is among the most flexible of Pinnacle s hardware devices because users can easily modify the configuration by changing only the preamplifier Standard configurations include 3 EEG 1 EMG channels and 4 EEG channels for seizure research For system and preamplifier specifications refer to pages 12 13 Users can also add a biosensor channel Any Pinnacle CNS biosensor can be used in conjunction with our four channel EEG EMG system for recording up to two simultaneous biosensor channels Adding biosensors enables the correlation of biopotential and neurochemical activity in freely moving mice and rats making the system perfect for unlocking new findings during sleep seizure and behavioral studies It is also possible for the system to acquire two biosensor inputs on a single channel This allows for the simultaneous capture of data from three biopotential and two biosensor signals Learn more about biosensors on pages 4 5 SYSTEMS FOR MICE SYSTEMS FOR RATS HARDWARE KIT HARDWARE KIT e 8 e y P 8400 K1 Mouse EEG E
13. monitor real time changes in neurochemical concentrations With Pinnacle s turn key electronic and software systems users can record and analyze second by second concentration changes of neurochemicals in freely moving animals Pinnacle currently offers Enzyme Layer Outer Diffusion Limiting Membrane Platinum lIridium Inner Selective glutamate glucose lactate Surface Membrane choline and ethanol Integrated Ag A biosensors Our biosensors function by the enzyme mediated processing of the 1 Analyte of 240 um Interest analyte of interest This results in the production of hydrogen peroxide that is then detected by oxidation at a Ptlr electrode 1mm Electroactive interferents _ Epoxy Tip 200 um present in the brain are Interferent excluded via passive selective membrane and Outer diffusion limiting membrane may not be present on all Pinnacle biosensors through active removal when necessary Our sensors are shipped within seven business days of order receipt COMMON USES and include a warranty Custom sensor sizes are also available in vivo monitoring of brain chemical microenvironments Contact Pinnacle for details Neurochemical monitoring during behavioral and physiological CNS BIOSENSOR CHARACTERISTICS Drug screening including neuropharmacological effects In Vivo Lifetime Limit of Detection Identification of biomarkers Glutamate 0 05 0 1 uM Invest
14. pinnaclet com info center html a representative about how our cutting edge tools can improve and simplify your research website Visit our www pinnaclet com conferences html for a complete list of events website at www pinnaclet com distributors html for an updated list PUBLICATIONS POSTERS LINKEDIN FORUM Many Pinnacle customers regularly publish their m Missed a conference Visit us online to review i Sign up for our Linkedln group to y E scientific work in peer reviewed journals This recent posters presented by Pinnacle and our network and share information with other space is dedicated to sharing our customers customers at Neuroscience AES Sleep and scientists researchers technicians research with other users of Pinnacle products i other major conferences and executives who are shaping the and systems biomedical research field CONFERENCES 2 DISTRIBUTORS x Pinnacle Technology regularly attends scientific Pinnacle Technology is proud to make OOD conferences and meetings hosted both in the our products available to international XN United States and internationally Please stop by customer base by teaming with a growing list EN f our booth at upcoming conferences to chat with of distributors from across the globe Visit our C L C
15. 36 91 4 BELLE HOPPER An integrated food hopper can be added to any Pinnacle 9 cage The stainless steel food hopper attaches to the NEED EXTRAS inside of the cage holds approximately 50 grams of food 12 standard pellets and dispenses one pellet at a Product time It reduces disturbance to the animal and requires little maintenance during long term experiments Add Water bottle with holder g long p a second food hopper to increase the amount of food mae inr 14 mounting plate 35 5 cm available Food Hopper 9066 26 18 mounting plate 45 7 cm MASTER CLOCK PINNACL E a KEY ATU High precision synchronization 3 TTL inputs outputs and pulse train output support Accurate to 1 second per month Product Kit cable The combination of a master clock Master Clock 9000 K8 and the Sirenia software suite allows users to perform a range of experiments The MAS CLOCK is a central timing source requiring precise synchronization while using for experiments that require high precision multiple video and data interfaces Because synchronization of multiple devices Our system it optimizes synchronization the master provides an accurate lt 1 ppm from 0 to 70 C clock is perfect for optogenetics behavioral clock and three TTL I O ports It communicates studies and other protocols requiring ultra with Sirenia data acquisition software using a USB precise timing ILLUMINATORS The
16. 5 x 30 5 cm allow rodents to freely move around a cage All cages are made of 4 clear acrylic and are suitable for use with most commercial cage washing equipment Multiple sizes are available Cages and accessories also support tethered experiments Cage stand adjustable to 24 tall 61 cm CAGE LID Cage lids allow for additional containment of Cage stand adjustable to 36 tall 91 4 cm 9009 RSD active animals Lids provide security and can be easily removed for cleaning and feeding CAGE EXTENDER Increase the height of our standard cages 3m using cage extenders The extenders fit snugly on top of Pinnacle s cages and add 4 per extension to cage height CAGE Pinnacle s cages are ideal for rodent research The circular design allows the animal complete freedom of movement around the circumference of the cage The open top makes cleaning and feeding easy and accessible One water bottle which attaches to the outside of the cage is included with each purchase For tethered setups our circular cage design prevents excessive slack and tension in the cable CAGE STAND Pinnacle s stands accommodate single animal cage and mounting plate The split design is compatible with both mouse and rat cages and all of Pinnacle s tethered recording systems There are two stand options They are adjustable to 24 61 cm and
17. 6 mm internal 4 mm external 2 8 12 mm variable external Max Resolution 640 x 480 pixels 640 x 480 pixels 1920 x 1072 pixels Max Frame Rate 30 fps 30 fps 30 fps Mount Above Above Side Tripod Above Side Tripod Pinnacles video camera setup highlighting camera options and IR Source Integrated Independent Independent mounting X configurations independent IR source is not shown Color Grayscale Both Both Both Interface Analog Analog Digital Ethernet CAMERA PACKAGES Accepts standard C mount lens Product Item Dome camera with integrated IR source 9000 K9 Box camera with independent IR source 9000 K10 SYNCHRONIZED VIDEO SYSTEM camera with independent IR source 9000 K11 Product Item s Additional HD cameras with independent IR source 9000 K12 Base Video Computer Package 9000 K1 Each camera package includes a camera IR source mounting Includes a preconfigured computer a high definition monitor a docking station accessories extension cable and one Sirenia license key for easy data transfer a keyboard and mouse and cables Up to four cameras can be added to a single video system Cameras are sold separately ADDITIONAL PRODUCTS Product Item Integrated video can be added to any Pinnacle recording system through Variable Focus Lens Box 9056 VF the Sirenia software platform Video is synchronized within 100 Tripod Box and HD 9059 milliseconds to simultaneous EEG EMG or biosensor data
18. DSE3 with 8235 SM In addition it contains 24 0 10 screws with wire leads 8403 instead of products 8209 8212 and 8226 Fully Independent Posterior 8200 K3 iSE3 EEG for Seizure 3 EEG for Mice Components of this kit are the same as above except 8201 is replaced with 8415 SM In addition it contains an 8 pin to 6 pin adapter 8272 and 28 0 10 screws with wire leads 8403 instead of products 8209 8212 and 8226 SYSTEMS FOR RATS HARDWARE KITS ACCESSORY KITS Anterior Configuration Sleep Seizure 8200 K4 SL SE 2 EEG 1 EMG for Sleep or Seizure SEEMS 200505 8112 Drill bit 8249 Test source Posterior iiia ESEE 8239 Rat headmount 4 8255 Multimeter e EEO T EMGor Rats Contents 8241 5 Screwdriver for 1 8 screws 9005 Powered USB hub 8206 Data conditioning and acquisition system 8213 Rat preamplifier 8214 Rat commutator swivel 8258 14 mounting plate 8247 1 8 screws with wire leads 16 8200 K4 SE3 3 EEG for Seizure Cables for one animal software and manuals are also included Components of this kit are the same as above except for the quantity of 8247 All accessory kits contain items needed NOTE Products 8206 and 8213 come in three varieties 24 In addition 8239 is replaced with 8239 SE3 for completion of four or six surgeries SL SE and SE3 All quantities are 1 unless otherwise noted after the product description FOR RATS HAVE YOUR OWN AMPLIFIER DISPOSABLE
19. ETHERED SYSTEMS FOR RATS AVAILABLE PREAMPLIFIER KITS 8400 K3 2BIO Preamplifier Kit for Two Biosensors HARDWARE KIT 8406 2 Mouse preamplifier for up to 2 biosensors 8209 0 10 screws 3 pkgs of 8 8400 K1 2BIO Tethered Mouse Biosensor System 7032 BASi mouse cannula 12 8241 F Screwdriver for mouse screws 7033 Bio only headmount 6 8254 23 gauge needle 6 8401 HS Data conditioning and acquisition system 7035 M BAS Probe holder for mouse cannula 2 9005 Powered USB hub 8408 Mouse commutator swivel 8134 Test load 2 8426 18 mounting plate 8400 K3 BIO Preamplifier Kit for One Biosensor Cables for one animal software and manuals are also included Components of this kit are the same as above except for the quantities of 7032 6 and 7035 M BAS 1 Biosensors sold separately FOR MICE Item 7032 BASI mouse guide cannula ADD SYNCHRONIZED VIDEO 7033 Bio only headmount See page 22 for details 8209 0 10 screws pkg of 8 23 gauge needle CNS SENSORS DESKTOP POTENTIOSTAT Pinnacle s recently redesigned FOUR CHANNEL DESKTOP POTENTIOSTAT provides a cost effective easy to use and highly accurate system for the development and use of high impedance amperometric biosensors and biosensor arrays It is well suited for anesthetized animal experiments brain slices and other in vitro studies Each of the system s four channels has one TTL inpu
20. G 1 EMG Wireless Sleep System for Rats 8266 SL 3 Channel Wireless EEG EMG Amplifier Transmitter for Rats 8266 SL Bat Battery for 8266 SL 9054 Bluetooth Dongle 9052 USB Cable Xx 8294 8266 Manual Pinnacle s wireless EEG EMG System 9028 Sirenia amp CD 9029 Sirenia Manual 9080 Zinc Air Battery Package of 4 ACCESSORY KIT SYSTEM MEASUREMENTS Transmitter Accessories 9005 Powered USB Hub Samples sec on each channel 200 8266 SL Bat Battery for 8266 SL Battery Life 36 hours 8107 WSL Rat Hat top for 8266 SL System Weight 239 8108 WSL Rat Hat bottom for 8266 SL longer studies the removable battery pack can be easily replaced without disassembling the head mounted enclosure Includes batteries enclosure and electronics 8241 S Screwdriver for Rat Bone EEG Screws 8112 Drill Bit for 1 8 Bone EEG Screws 8247 1 8 EEG Screw with Wire Lead for Rats 16 8270 2 EEG 1 EMG headmount for 8266 potentiostat 9080 Zinc Air Battery Package of 4 re 8156 Battery Remover plastic 8255 Multimeter 8249 EEG EMG Test Source 8271 RA 6 pin Connector Male Double Sided Adaptor EEG1 8235 6 pin Connector for Mice Straight Pin Configuration SEE OUR SIRENIA SLEEP PRO SOFTWARE Details on page 24 An example data set from the wireless EEG EMG system showing sleep transitions SLEEP DEPRIVATION SLEEP DEPRIVATION SYSTEM Pinnacle
21. M Denn 20 Tethered System Tor MICS qe 21 Wireless System TOF RAIS E vse Ds Dune 21 SYNCHRONIZED VIDEO 29 SOFTWARE NO 23 25 Sirenia VS scence we m m ede tc eect et art de ee en ne gk ac oe ceteris ces E EAE E Ea 23 24 SIONIS Selz ure PrO EE 24 Seale a ETe m mm 25 PACKING E ou RR DD pcd LR roo n n oen M Bu RERO EDU i CPU 25 Cages Stands Accessory sso Re EEEE 26 Master a 27 iluminators CE QS L LLL LLL LLL T EEEE GEES a AE EA EEES EEE S E EEEE E OEE Ea Ea 27 eRe e E E E E A EAE E AE N E EE E E E E E E EE E eee ee E E en eee E N 27 Arias Oa E E E A A A E OET IE E E N O E ANE A RIA 27 Swivels nba RES rte ION Dn I ROC tre tr err E c tr RR Hw Un rr Tr ret re rere err ec er ree rr Tr UR re rere terre er E n cre re 27 CNS SENSORS PINNACLE CNS BIOSENSORS CNS BIOSENSORS
22. MG System 8400 K2 Rat EEG EMG System P m w EE 8401 HS Data conditioning and acquisition system 8401 HS Data conditioning and acquisition system 4 EEG for Mice 8408 Mouse commutator swivel 8409 Rat commutator swivel 8426 18 mounting plate 8426 18 mounting plate Anterior Cables for one animal software and manuals are also included Cables for one animal software and manuals are also included 8400 K3 SE31M EEG 1 EMG for Seizure 8400 K4 SE4 4 EEG for Seizure Posterior 4 EEG for Rats 8400 K3 SE4 4 EEG for Seizure 8400 K4 SE BAS 2 EEG 1 EMG 1 Biosensor for Seizure 8400 K3 SE 2 EEG 1 EMG 1 Biosensor for Seizure 8400 K4 SL BAS 2 EEG 1 EMG 1 Biosensor for Sleep SIMPLE SURGERIES 8400 K3 SL 2 EEG 1 EMG 1 Biosensor for Sleep 8400 K4 SE3 BAS 3 EEG 1 Biosensor All preamplifier kits include one preamplifier one time purchase items connectors provide fast and easy solutions 8400 55 2 EEG 1 EMG 2 Biosensor for Seizure and surgical disposables to complete four surgeries for connecting electrodes to preamplifiers Stainless steel screws affix to the skull 8400 K3 5SL 2 EEG 1 EMG 2 Biosensor for Sleep doubling as anchors and electrodes for EEG data acquisition Depth electrodes can be 8400 K3 SE3 3 EEG 1 Biosensor COMBINED EEG EMG BIOSENSOR SYSTEMS soldered to connectors for LFP recordings 8400 K3 5SE3 3 EEG 2 Biosensor For configurations supporting muscle Lactate wn move
23. Page 15 Wireless System for Rats Page 16 e Cy SLEEP STUDIES SEIZURE RESEARCH gt DEPTH ELECTRODES CORTICAL RECORDINGS gt COGNITIVE STUDIES lt LOCAL FIELD POTENTIAL LOW NOISE SYNCHRONIZED TURN KEY VIDEO SYSTEMS ADVANTAGES ADVANCED e SIMPLE ANALYSIS TOOLS SURGERIES FREE ACQUISITION SOFTWARE Two channels of EEG data can be captured alongside EMG data using Pinnacle s three channel biopotential recording system 11 EEG EMG SYSTEMS TETHERED SYSTEM BREAKDOWN OUR TURN KEY SYSTEMS are engineered to deliver clean artifact free data EEG and EMG waveforms are amplified and filtered at the head of the animal by a preamplifier Signals are then passed through the low torque swivel to the data conditioning and acquisition system for final stage amplification and filtering Each channel in our three and four channel systems features independent adjustable gain and filter settings Data are collected using Pinnacle s free acquisition software Sirenia The software allows users to view EEG EMG recordings in user defined time periods manually score sleep and review seizure events All data can be configured for export to most spreadsheet and database programs and are compatible with our advanced analysis software packages See pages 23 24 for additional information on Pinnacle software DATA CONDITIONING AND ACQUISITION A data conditioning and acquisition system DCAS performs se
24. are Kit ADVANCED CGMS ANALYSIS SOFTWARE pr P il LT ELT NN Mmm a h rnajsuT4 i anne aa cy Manoa oles PM incited Data Piia oe wy ig vp 42b ERE on ij T ny Nah Ae N v Pan 7 TN iie CA Wa n ri Ih UY xr 180 a pa V PL i la IC UR e Dero AM 16 s AM DAM B ane DA 13 2014 ose TORA iia Ger ia 1100 0 BH E ad Bim 14 Ei n TA Topa 1 Bayt 100 100 De 169 a Diy 13 5585 2805 Our CGMS software module includes extensive analysis features to visualize data and create customized plots charts and graphs Generate graphs tailored to your data with user defined variables and settings EEG EMG SYSTEMS EEG EMG SYSTEMS THREE CHANNEL AND FOUR CHANNEL BIOPOTENTIAL TETHERED RECORDING SYSTEMS are available for sleep seizure and general behavioral paradigms in freely moving mice and rats Both EEG EMG systems use head mounted preamplifiers to produce exceptionally clean waveforms even during animal movement The four channel system provides all the great features of the three channel system along with an extra channel configuration flexibil
25. condary TETHERED S AN FOR RATS amplification and filtering before sending data to Pinnacle s Sirenia Acquisition software for collection via a USB connection SPECIFICATIONS 8401 HS Adjustable Sampling Rates 200 20 000 Hz Software Configurable Low Pass Filters 10 Hz 1 kHz Max Bit A DC 16 Q A Plastics One commutator is mounted above the 4 TTL Input Outputs and 4 Analog Outputs KES 8206 m 8401 5 cage The commutator s two plug setup allows for O even rotation of the rotor 8214 m 8409 PRODUCT PART NUMBER KEY 3 Channel System 4 Channel System CABLE An 18 tether from Plastics One connects the commutator to the preamplifier The cable s wires are protected by a metal spring coil mujnuunamuuumunununmuunununuuuunuuumunuuumunuuununzkn bnnmunmuEEEEHEREEEEESEHHEREEESEREERREHE PREAMPLIFIER Signals are amplified and filtered at the head of the animal using our preamplifiers which ensures the delivery of clean artifact free data A Plastics One muuumnuunuuuunuuuuzl minumuumuuuunEEHEEREEEEEEEEEEEREEREERE screw connector is used to secure the preamplifier to the head of the animal 8213 m 8407 LULA LLL ALL LU IAE UA UL UL ULL ALARM RAT HEADMOUNT Prefabricated rat headmounts use Plastics One fittings mounted on a 9 mm X 9 mm board with EEG or EMG electrode wires attached An additional two pin
26. de cannula for mice 7032 BASi guide cannula for rats 7030 Pinnacle single barrel cannula for rats 7040 S Pinnacle tri barrel cannula for rats 7041 S s LINEAR SELECTIVE FAST GLUTAMATE BIOSENSOR LINEARITY Current nA 0 5 10 15 20 25 30 35 40 45 50 Glutamate uM GLUTAMATE INTERFERENCE RESPONSE 2 uM 2 yM L Aspartic L Glutathione Acid lt 2 uM gt L Cysteine t gt 1 2 2 250 uM Tryptophan 2 uM i 2 yptop L Tyrosine L Glutamine Ascorbate gt lt 10 uM Glutamate Pinnacle biosensors are selective for the analyte of interest and are linear at normal in vivo oxygenation levels over a physiologically relevant range as shown in the diagrams above CNS SENSORS WIRELESS SYSTEMS FOR RATS TWO CHANNEL and THREE CHANNEL WIRELESS BIOSENSOR SYSTEMS are available for recording neurochemical concentrations in freely moving rats Both turn key systems use Bluetooth technology to wirelessly transmit data to Pinnacle s Sirenia Acquisition software via a USB dongle They provide a platform for high capacity biosensor studies and are well suited for behavioral experiments D Stereotaxically placed guide cannula s allow for the insertion of biosensors post surgery 9 The Rat Hat bottom is affixed to the skull with bone screws and dental acrylic It houses the wireless electronics ba
27. eighth edition of the Guide for the Care and Use of Laboratory Animals specifies narrow illumination limits The Pinnacle 9057 EN illuminator offers infra red IR and visible lighting that can be automatically synchronized to your standard lighting schedule or controlled via USB This illuminator can be programmed to provide true dawn dusk transitions and scheduled lighting variations By adding a photosensor the unit can automatically turn on or off with room lighting photoresistor cable or by adding a lux meter lux cable the unit will provide quantifiable measurements of illumination levels at the base of the cage and consistent lighting over multiple experimental setups The base version 9057 provides IR lighting for video Product Item Illuminator 9057 Enhanced Illuminator 9057 EN Lux Cable 9057 LUX Photoresistor Cable 9057 PHO This general purpose wireless two channel potentiostat can be used for a wide range of amperometric systems including lab on chip and biosensors Its isolated design makes it uniquely suited for in channel and end channel detection in capillary electrophoresis systems The system is powered by a standard 9V battery and uses Smart Bluetooth telemetry to a USB dongle for reliable data transfer The potentiostat is fully supported by Pinnacle s Sirenia software suite Product Kit Isolated Wireless Potentiostat 9000 K7 aiuto PINNACLE m ua KEY FEATURES Portable Sam
28. electrode is used for 4 EEG configurations 12 _ EEG EMG SYSTEMS HEAD MOUNTED AMPLIFICATION PINNACLE CLEANER DATA S27 HOW OUR PREAMPLIFIERS WORK GAIN AND HIGH PASS FILTERS Pinnacle s high gain preamplifiers perform 100 amplification X10 in seizure rat configurations of differential measurements between two electrodes Each channel also features 0 5 1 0 or 10 Hz high pass filters Use the chart below to identify the exact preamplifier specifications for each channel type in your selected configuration For example a 2 EEG 1 EMG preamplifier configured for seizure studies in mice has a gain of X100 on all channels 1 0 Hz high pass filters on the EEG channels and 10 Hz high pass filters on the EMG channel TETHERED SYSTEMS FOR MICE ENTCTTCCENMENSCEETITITE Seizure X100 1 0 Hz EEG Channel s Sleep X100 0 5 Hz Seizure X100 10 Hz EMG Channel SELLAM anne Sleep X100 10 Hz RAT CONFIGURATIONS BAIN HIGH PASS FILTERS A low torque commutator which is mounted above Seizure X10 1 0 Hz the cage allows for unencumbered freedom of movement EEG Channel s Sleep X100 0 5 Hz Rotational Torque 2 x 104 N m Seizure X10 10 Hz 8204 m 8408 EMG Channel Sleep X100 10 Hz Standard three and four channel preamplifiers have two Ane channels sharing a common electrode and either one or preamplifier Six insulated wires are banded two independent channels respectiv
29. ely Fully independent NOS ne aule iat 15 differential preamplifiers are also available See diagrams ideal for use with very small animals below Perspective Pins extending from preamplifier O O O O O O PREAMPLIFIER Signals are amplified and filtered at the head of the animal using our preamplifiers This ensures the delivery of clean artifact free data The mouse O O preamplifier connects to a headmount via a friction fit p 8202 8406 Three Channel System O O Standard Configuration For Mice Three Channel System Four Channel System Fully Independent Configuration Standard Configuration For Mice For Mice MOUSE HEADMOUNT CHANNEL 1 CHANNEL CHANNEL 2 CHANNEL 4 Prefabricated headmounts Six pin or eight pin reduce surgery time allow connectors support flexible for reproducible electrode electrode placement for placement and provide customizable cortical or aap een EMG CUSTOM CONFIGURATIONS AVAILABLE Contact a Pinnacle representative at 785 832 8866 13 EEG EMG SYSTEMS THREE CHANNEL SYSTEMS Pinnacle s THREE CHANNEL TETHERED SYSTEM allows researchers to simultaneously record three channels of EEG and or EMG data The data conditioning and acquisition system DCAS and preamplifier are preconfigured and ordered as a matching pair Standard configurations include 2 EEG 1 EMG channels for sleep or seiz
30. em Product 7030 BASi rat guide cannula 8111 16 1 8 screws pkg of 16 IT F M 7040 5 Single barrel cannula 8112 Drill bit 7041 5 Tri barrel cannula 9031 Battery for two channel pkg of 5 8108 BLE Rat Hat bottom for two channel 9065 8164 M Battery for three channel 8108 3C Rat Hat bottom for three channel CNS SENSORS TETHERED SYSTEM FOR MICE The MOUSE BIOSENSOR SYSTEM features configurable input channels to record neurochemical concentrations This tethered system employs a head mounted preamplifier for measuring up to two biosensors simultaneously in one animal providing a turn key solution for biosensor recordings in mice D Stereotaxically placed guide cannulas allow for the insertion of biosensors post surgery Prefabricated headmounts are affixed to the skull with dental acrylic and act as a connection port for the two channel biosensor preamplifier Head mounted preamplifiers house two connectors for biosensors The rigid connection ensures high quality artifact free data A low torque commutator allows for unencumbered freedom of movement m The data conditioning and acquisition system performs secondary amplification and filtering before sending data to Pinnacle s Sirenia Acquisition software for collection p KEY FEATURES _ LOW TORQUE WEIGHT 2 2 GRAMS HEAD MOUNTED AMPLIFICATION SUPPORTS UP TO 2 SIMULTANEOUS BIOSENSOR RECORDINGS Ww T
31. entire data set or a series of values for specific ranges of data Additional features include customizable data filtering data overlay capabilities and time period highlighting Users can easily zoom in and out to view detailed changes in the data Plus all charts and graphs can be saved as high resolution images for use in publications and presentations Pinnacle s visualization software is included with all Sirenia Sleep Pro and Seizure Pro purchases or can be purchased separately SUB Q EXP 59 Sub Q glucose sensor response in a Sprague Dawley rat plotted over a 2 day period blue when housed under a 12 hour light 12 hour dark cycle Lights on is represented by vertical 2 hr F4 hr yellow bars while lights off is represented by vertical grey bars The lines indicate daily glucose injections 1 g kg IP Daily blood glucose measurements were performed using an Accu Check blood glucose meter via tail vein sample Daily 24 hour sensor readings were calibrated to the Accu Check measurement using a one point calibration procedure Data were collected in 1 second intervals and plotted using a 60 point median filter Combination plot displaying simultaneous sleep state and brain energy analyte concentration changes over a 12 hour period Data include hypnogram top trace brain glucose concentration orange brain lactate concentration green first derivative of lactate concentration change blue
32. igating cognition behavior circadian cycles stress learning memory sleep seizure vigilance state and new drug effects O DATA A ai LOW ANALYTE CONSUMPTION CARBON FIBER SENSORS CFS are used in conjunction with Pinnacle s tethered and wireless potentiostats to measure the presence of total biogenic amines in the brain using fixed ADVANTAGES potential amperometry FPA They are also used with fast scan NO SAMPLE FAST POST PROCESSING cyclic voltammetry FSCV systems All Pinnacle CFSs require SAMPLE RATE an Ag AgCl reference electrode 7065 The sensors are 34 um in diameter and 0 5 mm in length FAST RESPONSE TIME CNS BIOSENSORS AND GUIDE CANNULAS CNS BIOSENSORS are purchased by cannula type and analyte of interest carbon fiber sensors are purchased by cannula type Pinnacle recommends the use of a guide cannula system for optimal results when implanting and recording from sensors in the brain of a freely moving animal We manufacture standard biosensor electrodes Pt Ir wire with an integrated Ag AgCl reference that are compatible with multiple guide cannula types Pinnacle s single barrel and tri barrel cannulas for rats BASi cannulas for rats and smaller BASi cannulas for mice Sensors with no cannula and custom sensors are also available for purchase All sensors sold by Pinnacle are for non human use only SINGLE BARREL FOR RATS End of Guide Cannula with Inserted Biosensor Guide Ca
33. ity and the added capability of incorporating simultaneous CNS biosensor measurements See the System Features chart to determine which system better fits your research needs Pinnacle also offers a three channel wireless system for rats TETHERED SYSTEMS SYSTEM FEATURES 3 CHANNEL 4 CHANNEL Available for both mice and rats i lt lt lt lt lt lt lt lt lt Optimized for sleep and seizure experiments No cable artifact Adjustable gain and low pass filters Sampling rate up to 20 000 Hz per channel Digital input output controls lt lt KK Analog output option Fully configurable channels CNS Biosensor support Reconfigure via preamplifier exchange Tethered System Breakdown amp How Preamplifiers Work Pages 12 13 Three amp Four Channel Systems Hardware amp Accessory Kits Pages 14 15 Add a Biosensor to EEG EMG Recordings
34. ment EMG leads easily insert into the All preamplifier kits include one preamplifier one time purchase yt JA 100 uM back or neck muscles items and surgical disposables to complete four surgeries tho RT EEG CNS biosensors sold separately 250 uV EMG 0 0 5 1 0 Hours 1 5 2 0 25 EEG and EMG waveforms are plotted simultaneously with calibrated biosensor traces for lactate and glucose recorded from a single animal FOR MICE Product tem Product ADD SYNCHRONIZED 8201 Mouse headmount 8235 SM 6 pin connector VIDEO 8201 SS Mouse headmount stainless steel 8254 23 gauge needle 8209 0 10 screws pkg of 8 8403 0 10 screws with wire leads See page 22 for details 8212 0 12 screws pkg of 8 8415 SM 8 connector 8226 Twin pack of silver epoxy 8431 SM 3 EEG 1 EMG headmount EEG EMG SYSTEMS WIRELESS EEG EMG SYSTEM FOR RATS The lightweight head mounted Bluetooth wireless Model 8266 for rats measures 3 biopotentials simultaneously and presents the data in real time for review The battery life is greater than 36 hours using off the shelf batteries For longer studies the removable battery pack can be quickly and easily changed The data are streamed to a computer using a small USB dongle receiver and the standard Sirenia software suite In addition our proven rat EEG EMG electrode placement system ensures fast setup and consistent reliable results HARDWARE KIT 8200 K6 SL 2 EE
35. mpatible with all of Pinnacle s hardware systems Order it as an accessory to Record in color or grayscale Record in low light or complete darkness a new system or easily integrate it into your current Pinnacle Flexible file size management Synchronized video with data recordings setup It can also be used as a stand alone video system Unrestricted video playback Record from any angle Box HD Camera options are described below lcg OPTION 1 OPTION 2 e Pinnacles dome camera mounts The box camera has improved optics and low light performance above the cage Its built in infrared compared to the dome camera as well as flexibility in mounting illumination adjusts to lighting options It can be mounted above the cage using Pinnacle s standard conditions automatically allowing mounting plate on the cage stand or to a tripod for recording at lower video recording in reduced lighting angles A separate automatic infrared illumination source allows and complete darkness video capture in conditions of low light and complete darkness OPTION 3 The high definition HD Internet protocol IP camera includes all the features of the box camera with the added capability of recording at six times the resolution The camera uses a single cable for power and data transfer FEATURES Box HIGH DEFINITION Lens 3
36. nnula Shaft 0 7 mm 2 mm from end of guide shaft to midpoint of sensing cavity Sensing Cavity 1 mm TRI BARREL FOR RATS 5 3 mm H 20 mm 10 5 mm 7 5 mm End of Guide Cannula with Inserted Biosensor 20 mm Guide Cannula Shaft 1 4 mm 11 mm 1 8 mm from end of guide shaft to midpoint of sensing cavity Sensing Cavity 1 mm O Ring End of Guide Cannula with Inserted Biosensor 19 5 Guide Cannula Shaft 0 7 mm 4 6 mm E 34 2 5 mm from end of guide shaft to midpoint 10 mm of sensing cavity S ensing Cavity 1 mm BASI FOR MICE 2 5 End of Guide Cannula with Inserted Biosensor Guide Cannula Shaft 0 65 mm 3 2 mm 71 3 mm from end of guide shaft to midpoint of sensing cavity Sensing Cavity 1 mm CNS SENSORS CNS SENSOR TYPES Product Item No cannula headpiece 7001 7001 Choline 7001 Glutamate 7001 CFS 7001 Ethanol 7001 Lactate 7001 CFS F 7001 Glucose BASi cannula headpiece for mice 7004 BASi cannula headpiece for rats wireless 7002 Pinnacle cannula headpiece for rats wireless 7005 ll BASi cannula headpiece for rats tethered 7011 7001 CFS Carbon Fiber Sensor gt 7001 CFS fixed in cannula All sensor types can be ordered in any of the analytes configurations listed under 7001 For example a glutamate biosensor with a BASi cannula headpiece for mice is ordered as 7004 Glutamate GUIDE CANNULA TYPES Product Item BASi gui
37. ogenetic stimulation is based on LEDs coupled to 62 5 125 uM fiber optic cable with a minimum light output of 10 mW mm The LED fiber probes are implanted using a guide cannula and standard stereotaxic techniques The LED fiber probe assembly plugs directly into a 2 mm electrical header on the headstage removing any requirements for an optical commutator HARDWARE AND ACCESSORIES The core hardware kit for mice includes all necessary items excluding the headstage The headstages are sold by kits which include all the supplies necessary for 4 surgeries and one time purchase items If you already own a Pinnacle Data Acquisition System it is compatible with the Optogenetics system HARDWARE KIT 8400 K11 Optogenetics Core Mouse System 8401 HS 4 Channel Data Conditioning amp Acquisition System 8480 Optogenetic Interface Module 8481 M Optogenetic Commutator for Mice 8413 M 6 15 pin Cable 8426 Mounting Plate 18 8260 Power Supply 9026 Power Supply Splitter Cable 9002 USB Cable 9008 USB Driver Install Disc amp Installation Instructions 8495 8400 User Manual 8496 Optogenetics User Manual 9028 Sirenia CD _ 9029 Sirenia Manual HEADSTAGE KITS 8400 K13 O1 1 Opto Headstage Kit 8400 K13 O2 2 Opto Heads
38. on Fiber Sensors Pinnacle Technology Inc requires seven business days notice prior to requested date of shipment for biosensor or carbon fiber sensor orders Payment Terms Net 30 days from date of invoice for customers with established credit Prepayment or COD may be required if credit has not been established Major credit cards are accepted Unpaid balances are subject to a late payment fee of 1 596 per month Pro forma invoices are available for international orders Use of Products All Pinnacle Technology Inc products are sold for laboratory research use only Pinnacle Technology Inc products have not been approved by any government agency for use in human subjects or human testing Shipping Information United States All orders ship F O B Lawrence KS Standard orders are shipped FedEx Ground biosensor orders are shipped FedEx 2 Day Freight charges are added to the final invoice International Purchaser is responsible for payment of all import duties tariffs taxes insurance and other related charges Pinnacle Technology Inc ships via the purchaser s courier of choice UPS FedEx DHL using the purchaser s courier account number Orders WILL NOT BE SHIPPED without this information Pinnacle Technology Inc accommodates orders shipped through domestic shipping brokers Product Price Notices Prices and specifications are subject to change without notice Product Return Policy All product returns require a
39. ording The glucose sensor penetrates the animal s subcutaneous space on the dorsal surface and is held in place with four surgical sutures The system uses a jacket to secure the pouch and stabilize the sensor HARDWARE KITS HARDWARE SPECIFICATIONS 8100 K5 BP 2 Channel Potentiostat Kit for Rats RATS MICE 8164 2 channel LE Bluetooth wireless potentiostat backpack System Weight 5 9 grams 3 8 grams goa CAERON CARIE Battery Life 25 days 17 days 9054 Smart Bluetooth dongle 3 Channels 2 1 9080 Zinc air battery Package of 4 ES Transmission Radius 20 feet 20 feet 8100 K11 BP 1 Channel Potentiostat Kit for Mice Components of this kit are the same as above except 8164 is replaced m with 8164 M and 9080 is replaced with 9065 8164 M ACCESSORY KIT 8100 7 CGMS Accessories 8134 Test load Raw One Second Data 8156 Battery remover plastic 8165 Rat jacket 8166 Electronics pouch 8167 Suture packets 3 0 silk 8168 Tegaderm 1624W 6cm x 7cm 8169 18 g needle 9005 Powered USB hub 9080 Zinc air battery Package of 4 Within the Pinnacle CGMS environment data are under the control of the researcher Raw 8100 K12 BP CGMS Accessories for Mice per second samples and filtering capabilities are available The above graph represents 48 hours A of data grey bar
40. ox that streams data to the host PC HARDWARE KITS ACCESSORY KITS 8500 K1 FSOCV Mouse System tethered 8500 K3 FSCV Accessories for Mice tethered 8408 Mouse commutator 7033 Bio only headmount 4 8254 23 gauge needle 4 MOUNINO pla lll IOS pope Pinnacle s tethered FSCV system 8503 Tethered FSCV headstage 7037 Probe clamp accessory 8510 100M test load 8504 FSCV interface box 7039 Allen wrench 9005 Powered USB hub Cables for one animal software and manuals are 8212 0 12 screws pkg of 8 9033 NiMH batteries pkg of 4 also included 8241 F Screwdriver for mouse screws 9034 Battery charger CALIBRATION KIT KEY FEATURES SWEEP RATE 10 SWEEPS SECOND HEAD MOUNTED AMPLIFICATION 1000 POINTS SWEEP LOW TORQUE SWIVEL FSCV Calibration Kit 8500 K5 WIRELESS SYSTEM FOR RATS Used for mouse and rat systems The WIRELESS RAT SYSTEM uses Bluetooth to transmit data The system resides in a head mounted enclosure and wirelessly transmits data to a computer The battery is easily accessible and readily exchangeable in real time to support extended recordings The system is ideal for mazes and enclosed environments such as metabolic and behavioral chambers HARDWARE KITS ACCESSORY KITS 8500 K2 FSCV Rat System wireless 8500 K4 FSCV Accessories for Rats wireless 8501 Wireless ESCV board 7036 Clamp rod 8506 Battery with cover
41. pid data analysis All EEG EMG and video data sets recorded with Pinnacle software as well as third party EDF files can be imported for analysis SIRENIA SLEEP PRO Sleep Pro provides three mechanisms for scoring sleep data manual cluster and threshold scoring Combine multiple methods to quickly and accurately score both mouse and rat files Epoch lengths are user configurable and numerous scoring sessions can be created for the same file In addition real time hypnograms epoch by epoch heat maps and spectral plots are available as visual aids Plus powerful analysis tools such as sleep stage sleep bout analysis and user score comparison make reviewing and exporting data easy KEY FEATURES SIRENIA SEIZURE PRO Seizure Pro employs a database system to store line length and power characteristics of user defined seizures Information collected in the database is used to quickly identify and mark like events within any Sirenia or EDF file Racine s scale ratings seizure classification and notes can be easily added to logged events for future reporting Plus spectral plots and heat maps are available to aid in visual confirmation Seizure statistics including average duration time between seizures and peak frequency are automatically generated KEY FEATURES Multiple scoring tools Heat maps Real time hypnograms Power analysis Spectral plots Peak frequency analysis ACCURACY OF SLEEP DETECTION
42. ple rate 13 Hz Bias range 0 4 V Resolution 24 bits Bluetooth transmission Transmission range 6 m Current range 80 uA Battery life 100 hrs continuous use Pinnacle s mouse commutators are ideal for use in studies that require a low torque Analog adapters are excellent solutions for researchers who have existing amplification electrical swivel The lightweight nature of our commutators provides a platform to transfer acquisition systems but require the noise reduction provided by Pinnacle s head mounted electrical signals without significantly altering the behavior of the animal Custom adapters preamplifiers and commutators The adapters can be used to connect Pinnacle mouse and can be ordered for integration into existing third party cabling connection schemes rat preamplifiers with third party data collection systems Available for mice only Product Kit 3 Channel Analog Adapter 8242 K 4 Channel Analog Adapter 8442 K Product Six pin commutator Nine pin commutator Ten pin commutator Item 8204 8408 8481 M Cables software and manuals are included in all kits 21 LEARN MORE Pinnacle s products are used daily to advance research at academic institutions research hospitals government laboratories contract research organizations and pharmaceutical companies across the world Learn more about how current customers are using our products by visiting the Info Center on our website at www
43. r running the program at a time Purchase includes one year of free upgrades Contact Pinnacle at sales pinnaclet com for additional package options Download free software trials at www pinnaclet com software html THIRD PARTY DATA STREAMS In addition Sirenia supports the integration of third party data streams via a National Instruments I O module These data can be simultaneously recorded along with Pinnacle s biopotential and biosensor data Product Item National Instruments I O module 9032 SEIZURE PRO Quickly identify and analyze events in your data using the line length and power characteristics of user defined seizures SLEEP PRO Score mouse and rat data in 75 less time using automated tools such as cluster and threshold scoring VISUALIZATION Manipulate and view data with our innovative graphical analysis software Precisely track animal movement in real time and quickly analyze behavioral patterns based on trajectories quadrant analysis and much more CGMS Monitor and analyze your preclinical glucose data with our software specifically tailored for CGMS experiments LEARN MORE ABOUT THESE TIME SAVING MODULES PAGES 24 25 23 SOFTWARE PREMIUM SOFTWARE MODULES FOR SLEEP AND SEIZURE Upgrade from our basic software to automate your sleep scoring and seizure identification processes Both SIRENIA SLEEP PRO and SIRENIA SEIZURE PRO provide automated tools for ra
44. s AUTOMATED SLEEP DEPRIVATION SYSTEM is a unique solution for sleep deprivation and fragmentation studies It provides user controls for sleep depriving mice and rats without direct human intervention Sleep deprivation simulating gentle handling is achieved by a rotating bar placed a short distance above the cage floor lightly nudging the animal from sleep and encouraging the animal to maintain wakefulness without excessive exercise The system is sold either as STAND ALONE or with FEEDBACK The feedback version includes all the features of the stand alone system In addition EEG EMG signals can be used to determine sleep wake state and initiate deprivation as required It includes a license key for unlocking Sirenia s versatile sleep deprivation settings TWO SLEEP DEPRIVATION SYSTEMS Available for both mice and rats Adjustable speed and motor control Calendar based scheduling Suitable for short term long term and chronic studies ENNBESSNBS Optional video recording Real time biopotential analysis and feedback Rule based programming Yoked control functionality SISISISISISISISIS Requires Pinnacle s EEG EMG system Compatible with third party systems v ADVANTAGES Simulates gentle handling Reduces unnecessary exercise Minimizes resources compared to manual deprivation Prevents sleep acclimation and habituation AUTOMATED SLEEP DEPRIVATION SYSTEMS Product Item Stand Alone for Mice 9000 K5 S
45. s designed to optically trigger genetically altered neurons in one or more specific brain regions with simultaneous recording of electrophysiological signals or neurotransmitter concentration changes The key component is an Optogenetics Interface Module which provides precise timing and illumination control when integrated with our existing 3 and 4 channel data acquisition systems This connects to an electrical commutator and a headstage overall design is similar to diagram on page 12 The system uses LED fiber probes which are compatible with standard cannula placement techniques and do not require an optical commutator Four headstages are currently available 1 or 2 channel stimulation only for behavior studies 1 channel stimulation plus EEG EMG for sleep studies or 1 channel stimulation plus EEG for seizure research Includes a stable highly accurate clock to ensure lt 10 us timing accuracy for all events controlled directly by module Interfaces with all Pinnacle Data and Acquisition Systems 8200 amp 8400 Precise current and pulse timing control for the fiber optic probes current control and pulse timing can also be provided by an external source 3 digital inputs 1 digital output 2 isolated inputs for other external stimuli Stimulated Hemisphere B Non Stimulated Hemisphere 150 100 50 Percentage of Baseline NREM Delta Power 0 3 Hours of Recovery LED FIBER PROBES Pinnacle s opt
46. t one TTL output and one analog output The potentiostat is compatible with Pinnacle s biosensors and third party sensors KEY FEATURES 4 channels Maximum sampling rate 4 Hz 2 terminal fixed potential Current range 0 20 uA Resolution 3 fA Bias range 2 048 to 2 048 V HARDWARE KIT 8100 K4 Desktop Potentiostat System 8102 Desktop potentiostat 8109 Sensor adapter cables 4 8118 Power supply 8124 Shorting cap 8125 BNC to alligator leads cable 4 4 TTL inputs 8155 BNC test load 4 4 TTL outputs 9005 Powered USB hub 4 analog outputs Cables software and manuals are also included COMPATIBLE WITH THIRD PARTY SENSORS CALIBRATION KITS To relate the in vivo current changes measured by a CNS biosensor to actual changes in analyte concentration it is necessary to calibrate the biosensor at the conclusion of the in vivo experiment Pinnacle offers a number of in vitro calibration systems that allow the simultaneous calibration of four to eight biosensors Product CALIBRATION KITS Kit Tethered mouse in vitro calibration kit 7000 K1 T Wireless rat in vitro calibration kit use with BASi cannulas 7000 K2 W BAS Wireless rat in vitro calibration kit use with Pinnacle cannulas 20 08 _ 1 g kg EtOH _ w 15 5
47. tage Kit 8400 K13 O1 2EEG 1EMG 1 Opto 2 EEG 1 EMG Sleep Headstage Kit 8400 K13 O1 3EEG 1 Opto 3 EEG Seizure Headstage Kit Most National Instruments devices are mapped as virtual devices which can then be accessed within Sirenia This capability allows researchers to use existing equipment to supplement a wide range of high temporal accuracy optogenetics experiments OPTOGENETICS LED FIBER PROBES FOR MICE End of Guide Cannula 2 5mm _ Guide Cannula Shaft 0 65 mm 3 2 mm Fiber Length 0 5 mm Product Item LED Fiber Probe 470 nm Wavelength 7080 470 3 EXPERIMENTAL DESIGN An optogenetics experiment is driven by the placement of the optical stimulation in the brain Based on the researcher s requirements the choice of a headmount is critical Two examples are shown below for a sleep experiment The LED Fiber Probe can be placed in either hemisphere of the brain LED Probe LED Probe For same hemisphere recordings use the 8402 SS headmount CUSTOM PROBES AVAILABLE FAST SCAN CYCLIC VOLTAMMETRY SYSTEM Pinnacle s FAST SCAN CYCLIC VOLTAMMETRY FSCV SYSTEM is robust turn FOCUS key and specifically designed to simplify the measurement of catecholamines i e eae ENERGY REINFORCEMENT AROUSAL MOOD dopamine norepinephrine and serotonin It functions by rapidly cycling a voltage
48. ttery guide cannulas and biosensors A low powered wireless two or three channel potentiostat applies a bias and transmits up to three digitized signals to a paired Bluetooth USB dongle that interfaces with Pinnacle s Sirenia Acquisition software for data recording VT10 0 08 LNV 4 The Rat Hat top protects the system UP TO THREE CHANNELS UNTETHERED FREELY MOVING ANIMALS TRANSMISSION RADIUS 6 METERS SUPPORTS SIMULTANEOUS BIOSENSOR RECORDINGS T e 8100 K10 Three Channel Wireless Biosensor Accessories 7036 Probe clamp assembly 8111 16 1 8 screws pkg of 16 HARDWARE KITS 7040 S Single barrel cannula with stylet 6 8112 Drill bit 2 7041 S Tri barrel cannula with stylets 2 8134 20 20 M test load 2 8100 K9 Three Channel Wireless Biosensor System 7043 Stereotax holder for tri barrel cannula 8147 A Hex screwdriver 8173 Bluetooth potentiostat 7044 Stereotax adapter 8156 Battery remover 9052 USB extension cable 7045 Hex screws for probe clamp 8241 S Screwdriver for 1 8 screws 9054 Bluetooth dongle 8107 3C Rat Hat top 9005 Powered USB hub 8100 K5 Two Channel Wireless Biosensor System 8108 3C Rat Hat bottom 4 9065 8164 M Battery 8 8100 K7 Two Channel Wireless Biosensor Accessories ALSO AVAILABLE AS A BACKPACK Kit contents for the two channel biosensor system are available on our website Biosensors sold separately FOR RATS DISPOSABLE It
49. ure studies and 3 EEG channels for seizure research Fully independent preamplifiers and 3 EEG kindling systems for rats are also available Learn more about preamplifiers and how our turn key systems work on pages 12 13 SYSTEMS FOR MICE ACCESSORY KITS 8200 K3 SL SE 2 EEG 1 EMG for Sleep or Seizure HARDWARE KITS 8201 Mouse headmount 4 8249 Test source Anterior Configuration Sleep Seizure 8209 0 10 screws pkg of 8 8254 23 gauge needle 4 2 EEG 1 EMG 8200 K1 SL 8200 K1 SE 8212 0 12 screws pkg of 8 8255 Multimeter 2 EEG 1 EMG 8200 K1 iSL 8200 K1 iSE 8226 Twin pack of silver epoxy 2 9005 Powered USB hub 3 EEG 8200 K1 SE3 8241 F Screwdriver for EEG screws m Posterior 3 EEG 8200 1 15 8200 K3 iSL iSE 2 EEG 1 EMG for Sleep or Seizure 2 EEG 1 EMG for Mice Contents Components of this kit are the same as above except 8201 is replaced with 8431 SM In addition it contains an 8 pin to 6 pin adapter 8272 and 20 0 10 screws with wire leads 8403 instead of products 8209 8212 and 8226 8202 Mouse preamplifier 8204 Mouse commutator swivel 8206 Data conditioning and acquisition system 8258 14 mounting plate Anterior 8200 K3 SE3 3 EEG for Seizure Cables for one animal software and manuals are also included NOTE Products 8202 and 8206 come in multiple varieties Components of this kit are the same as above except 8201 is replaced SL SE SE3 DSL DSE and
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