X-Cite® XLED1
Contents
1. 2260 Argentia Road Telephone 1 905 821 2600 Ly LUMEN DYNAMICS Mississauga Ontario Toll Free USA and Canada 1 800 668 8752 L5N 6H7 CANADA Facsimile 1 905 821 2055 PUTTING YOU IN CONTROL www LDGI XCite com X Cite LDGI com SZ 44745 o Rm A 4 7160 0014 RRI EKRARE 1 Bih ARBEIT 1 YT EFA 7 TA F FS TEL 03 3356 1064 FAX 03 3356 3466 E mail info optoscience com AN P sS A Be SM E 7532 0011 ARRAS MAME hy RASA P P H L LE A A TEL 06 6305 2064 FAX 06 6305 1030 E mail osk optosci ence com http www optoscience com AHER 17450 0002 2h BmPHRAER2 37 21 RYZ kE TEL 052 569 6064 FAX 052 569 8064 E mai ngo optoscience com2. Resetting the standard for LED illumifiiion Optimized excitation with high power LED illumination at the sample plane Instant switching between wavelengths ideal for multi color live cell imaging High Speed automation with advanced internal pulse generation and external triggering options Ease of use through an optional interactive touch screen controller and intuitive user interface Flexible and scalable to allow you to tailor to specific applications PUTTING YOU IN CONTROL 8 LUMEN DYNAMICS X Cite The X Cite Advantage Designed with researchers in mind the X Cite XLED1 combines high power excitation specificity with high speed imaging G a f for maximum control versatility flexibility and ease of use Inspired by over 27 years of light applications expertise X Cite XLED is the superior choice in LED technology ideal for microscopy applications involving fast automated multi color live cell imaging Reliability with long lifetimes Fast on off switching with no warm up required Elimination of frequent mercury and xenon lamp changes No need for mechanical shutters prevents vibration and increases imaging speed Optimized excitation through narrow bandwidth and diverse wavelengths Reduced phototoxicity and bleaching Compact size low heat generation and energy efficiency Stability spatial and temporal Increased excitation specificity without the high cost of lasers
3. 8 5 W x 8 H x 15 D 22cm x 20cm x 39cm Weight 7 3kg 16lbs full system Supply Universal input of 100 240Volts AC 50 60Hz Fused at 6 3A Connectivity USB type B female Trigger Input BNC TTL compatible Sync Output BNC TTL compatible Triggering Internal External User defined per channel Global trigger input User defined free running or single shot pulse modes Internal triggering generated from internal pulse generator 10uUs min up to 18 hours max Sync Out Mapped echo of LED cycles on separate output pins Sync Out Phase Control Individual control of sync phase advance retard half of max cycle duration Switching Speed Instantaneous Minimum Duty Cycle 10us X Cite XLED1 Touch Screen Controller Optional Screen Medical Industrial grade 7 TFT viewable diagonal Resistive touch screen with 16 9 aspect ratio and LED backlight with adjustable intensity Resolution 800 x 480 pixels Display Viewing Area 6 W x 3 5 H 15cm x 9cm External Dimensions 8 W x 2 5 H x 5 5 D 20cm x 7cm x 14cm Program Lumen Dynamics proprietary graphical user interface Connectivity 2 5m connectorized power and data combo cable Power lt 10 watts Certifications CE Marked Certified to IEC Canadian and US standards ROHS compliant Warranty X Cite XLED1 System XLED1 touch screen controller and XLED1 LED drivers 12 months from shipping LED modules 20 000 hours or 3 years
4. Uniform and stable high power LED illumination Narrow LED bandwidth that reduces crosstalk between fluorophores Efficient wavelength mixing for uniform and powerful illumination of up to four fluorophores at the sample plane Fast triggering both internally and externally with pulse duration as short as 10us for advanced system control Instantaneous wavelength switching for fast multi color imaging applications Ultra fine control of intensity 0 1 for precise adjustments to output power Ease of use for a novice or an expert microscopist Intuitive intelligent GUI offering full system control via PC or optional touch screen controller Simple plug and play modules allowing fast and easy system reconfiguration Compatibility with third party software for simplified integration into existing experimental set ups 20 000 hours or 3 years LED module guarantee Harnessing the intrinsic advantages of solid state LED technology the X Cite XLED1 provides unprecedented wavelength and power stability over extended lifetimes Integrated with state of the art technology that provides full control over LED intensity and signaling the X Cite XLED1 can trigger or be triggered by external hardware By combining these features with an easy to use intelligent GUI or optional controller the XLED1 offers maximum control with the convenience and versatility of an X Cite The X Cite XLED1 offers up to four high powered LEDs
5. with narrow spectral profiles that enable excitation specificity while maintaining high power at the peak excitation wavelength via efficient optics including optimized microscope adaptors In certain applications excitation filters may not be required and the use of multi band filters negates the need to rely on a filter wheel thus enabling high speed imaging The X Cite XLED1 is well suited for applications including live cell imaging high speed or long term optogenetics and even fixed cell imaging involving fluorescent probes conjugates and the ever popular fluorescent proteins FP s along with the Figure 1 LED Spectral Profiles 365nm 385nm 405nm 447nm 460nm 525nm 635nm 660nm 735nm Normalized Spectra Wavelength nm We foun flexible fluoresc Holly Aaron standard Alexa ATTO dyes and traditional DAPI FITC and Cy dyes The XLED1 system maximizes excitation specificity to which laser users are accustomed but without the high cost of lasers Figure 1 The X Cite XLED1 has been designed with an optional touchscreen controller featuring an intuitive interface for easy navigation and advanced system control Figure 2 With a touch screen display users have the ability to view LED and system status while ensuring peace of mind when exchanging modules Advanced operators and lab managers can man
6. age more complicated imaging protocols through the triggering screen Figure 3 A unique virtual oscilloscope feature plots all programmed channel and trigger waveforms Figure 4 ensuring proper system set up as well as potentially eliminating the need for external electrical or optical test equipment A PC version of this GUI is also included with every X Cite XLED1 For those who choose to control the system through their own software a full SDK is available as well as drivers for common software packages The X Cite XLED1 system allows operators to control the intensity of each LED in large steps through a slider or through smaller steps using simple up and down arrows associated with each LED With the touch of a button intensity levels used from past experiments can be set via a numerical value of the required intensity The XLED1 touch screen controller also offers the ability to save optimized settings for future experiments with up to five user profiles that are password protected By simply logging in when starting an experiment and logging out at the end of the day individual settings will be saved for future use d the new XLED1 LED light source from Lumen Dynamics to be extremely n a multi user environment providing great excitation for all of our favorite ent proteins olecular Imaging Center UC Berkeley The X Cite XLED1 system is ideal for single or multi color live cell imaging including high speed applic
7. ations long term development imaging protein activation and interactions FRET and the popular optogenetics techniques With super fine control of power intensity as little as 0 1 increments users can fine tune the exact power required for optimal imaging With instantaneous switching between wavelengths and ability to pulse each LED for a defined time period or continuously the XLED1 system will allow you to image for long time periods while minimizing photobleaching and phototoxicity when compared to alternative light sources Designed with a sophisticated internal pulse generator the X Cite XLED1 allows full control of the LEDs according to experimental needs Signals can also be directed into each or all of the LEDs through TTL inputs generated by external hardware connected to the XLED1 Timing protocols can be defined as per experimental design to turn each LED ON and OFF as well as transmit trigger signals to peripherals e g stages cameras electrophysiology rigs connected to the XLED1 The X Cite XLED1 can be configured specifically for each user s applications Simply choose two or more LEDs and our online configurator will help you choose the dichroics required for the LED combination and help you determine the system for your needs The XLED1 offers easy replacement of LEDs with additional modules that may be required for niche applications for greater flexibility Just simply plug and play no wi
8. own to 10us Eliminates vibration and latency of mechanical shutters allowing precise short exposure times to limit photobleaching and phototoxicity Flexibility with experimental design and system control Easy configuration according to user application High efficiency coupling to a wide variety of microscopes currently available with X Cite products Reliable and consistent image data Ease of use no advanced training required GUI verifies system set up and ensures worry free module exchange Repeatable illumination and optimal imaging Figure 3 X Cite XLED1 offers superior power output and advanced control for high speed automated microscopy in LED fluorescence illumination Designed with researchers in mind the X Cite XLED1 combines excitation specificity and high speed imaging capabilities for maximum control versatility and ease of use Recognized as the industry standard in fluorescence illumination X Cite offers the XLED1 as the next generation of advanced LED technology for microscopy applications SPECIFICATIONS X Cite XLED1 package includes X Cite XLED1 illumination system optional XLED1 touch screen controller up to four modules user manual CD with GUI and USB driver quick start guide liquid light guide microscope adaptor power cord trigger cables USB cable X Cite XLED1 System Wavelengths Wavelengths available at http www LDGI XCite com downloads misc spectra pdf Dimensions
9. res to reconnect and no need for special tools Optical table visualization Figure 5 on the maintenance screen provides researchers with useful system configuration information and also pinpoints the location and details of each LED source and dichroic making reconfiguration straightforward X Cite is recognized as the industry standard for fluorescence illumination and was the first to introduce a commercial replacement for traditional HBO systems in 2002 The X Cite XLED1 represents a new generation of LED microscopy illuminators with optimum LED power where it is most essential at the sample while offering the convenience and ease of use researchers have come to expect from an X Cite What X Cite XLED1 offers How researchers benefit High power at the sample plane 365nm 735nm modules Narrow LED bandwidth Instantaneous wavelength switching Fast ON OFF Advanced triggering and pulsing options Configurable plug and play modules Optimized X Cite adaptors Uniform and stable illumination Intuitive GUI and interactive touch screen Easy module exchange 0 1 intensity control increments Figure 2 Increased spectrally targeted excitation power where it matters most to excite uncage activate fluorophores at the sample High signal to noise ratio reduces crosstalk between fluorophores leading to high signal specificity Enables fast multi color image acquisition and pulsing applications d
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