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1260 Infinity Standard Autosampler User Manual

1. The six port injection valve unit only 5 ports are used is driven by a high speed hybrid stepper motor During the sampling sequence the valve unit bypasses the autosampler and directly connects the flow from the pump to the column During injection and analysis the valve unit directs the flow through the autosampler This ensures that all the sample is completely injected into the column Any sample residue is removed from the metering device and from the needle before the next sampling sequence begins For applications that require control of the vial temperature the module can be combined with the Agilent 1290 Infinity Thermostat G1330B The combination of the autosampler with the thermostat is called a thermostatted autosampler For more details refer to the documentation of the 1290 Infinity Thermostat Agilent 1260 Infinity Standard Autosampler User Manual 11 1 12 Introduction to the Autosampler ALS Sampling Sequence The autosampler processor continuously monitors the movements of the autosampler components during the sampling sequence The processor defines specific time windows and mechanical ranges for each movement If a specific step of the sampling sequence can t be completed successfully an error message is generated During the sampling sequence the solvent bypasses the autosampler via the injection valve The gripper arm selects the sample vial either from a static sample rack or from external vial
2. tf Agilent Technologies Notices Agilent Technologies Inc 2007 2012 2013 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61329 90015 Rev B Edition 04 2013 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have
3. Probable cause Suggested actions 1 Defective injection valve Please contact your Agilent service representative 2 Defective main board Please contact your Agilent service representative 90 Agilent 1260 Infinity Standard Autosampler User Manual Error Information 7 Needle Up Failed Error ID 4017 The needle arm failed to move successfully from the seat or out of the vial to the upper position The upper position of the needle arm is monitored by a position sensor on the sampling unit flex board The sensor detects the successful completion of the needle movement to the upper position If the needle fails to reach the end point or if the sensor fails to recognize the needle arm movement the error message is generated Probable cause Suggested actions 1 Defective or dirty position sensor Please contact your Agilent service representative 2 Defective motor Please contact your Agilent service representative 3 Sticking spindle assembly Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative Agilent 1260 Infinity Standard Autosampler User Manual 91 7 92 Error Information Needle Down Failed Error ID 4018 The needle arm failed to move down into the needle seat The lower position of the needle arm is monitored by a position sensor on the sampling unit flex board The sensor detects the successful completion of the needle movement
4. This limit is given by 2 revolutions second for longer than 5 seconds Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Agilent 1260 Infinity Standard Autosampler User Manual Error Information 7 Open Cover Error ID 0205 The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during Please contact your Agilent service operation representative 2 Foam not activating the sensor Please contact your Agilent service representative 3 Defective sensor or main board Please contact your Agilent service representative Restart Without Cover Error ID 2502 The module was restarted with the top cover and foam open The sensor on the main board detects when the top foam is in place If the module is restarted with the foam removed the module switches off within 30 s and the error message is generated Probable cause Suggested actions 1 The module started with the top cover and Please contact your Agilent serv
5. 0081 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Agilent 1260 Infinity Standard Autosampler User Manual 85 7 86 Error Information Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the main board in the module has failed short circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated
6. A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent 1260 Infinity Standard Autosampler User Manual 147 10 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL 7 White 1 Digital ground a Ol ou Brown 2 Prepare run Low 00 pes DE KEY Gray 3 Start Low oo o0 S iE Blue 4 Shut down Low og Pink 5 Not oa connected 5 5 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 148 Agilent 1260 Infinity Standard Autosampler User Manual BCD Cables Identifying Cables 10 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 pea Violet 2 BCD 7 80 AA Blue 3 BCD 6 40 a ee Yellow 4 BCD 4 10 Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9
7. ALS Sampling Unit The sampling unit comprises three main assemblies needle drive metering device and injection valve NOTE The replacement sampling unit excludes the injection valve and metering head assemblies Needle drive Metering device Injection valve Figure 6 Autosampler Sampling Unit Agilent 1260 Infinity Standard Autosampler User Manual 15 1 16 Introduction to the Autosampler ALS Needle Drive The needle movement is driven by a stepper motor connected to the spindle assembly by a toothed belt The circular motion of the motor is converted to linear motion by the drive nut on the spindle assembly The upper and lower needle positions are detected by reflection sensors on the sampling unit flex board while the needle in vial position is determined by counting the motor steps from the upper needle sensor position Analytical head The analytical head is driven by the stepper motor connected to the drive shaft by a toothed belt The drive nut on the spindle converts the circular movement of the spindle to linear motion The drive nut pushes the sapphire piston against the tension of the spring into the analytical head The base of the piston sits on the large bearing of the drive nut which ensures the piston is always centered A ceramic ring guides the movement of the piston in the analytical head The home position of the piston is sensed by an infra red sensor on the sampling unit flex boa
8. Autosampler G4226A ALS 2 Yes No Yes No Yes Agilent 1260 Infinity Standard Autosampler User Manual 159 11 Hardware Information Table 12 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes K1314F Clinical Ed G4212A B DAD 2 No Yes Yes 1 Yes K4212B DAD Clinical Ed G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 2 Yes K1321B FLD Clinical Ed G1321C FLD G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No 1 G1316A C TCC 2 No No Yes No Yes K1316C TCC Clinical Ed G1322A DEG No No No No No Yes AUX K1322A DEG Clinical Ed G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes K4225A DEG Clinical Ed 160 Agilent 1260 Infinity Standard Autosampler User Manual Hardware Information 11 Table 12 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote G4227A Flex Cube 2 No No No No No CAN DC OUT for CAN slaves 1 G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED K1330B 1 Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or wit
9. Overview of the Module s Indicators and Test Functions 56 Status Indicators 57 Maintenance Functions 59 ALS Step Commands 66 Agilent 1260 Infinity Standard Autosampler User Manual Contents Troubleshooting 68 Troubleshooting Guide for the Sample Transport Assembly 70 Agilent Lab Advisor Software 77 Error Information 79 What are Error Messages 81 General Error Messages 82 Autosampler Error Messages 88 Maintenance 101 Introduction to Maintenance 102 Cautions and Warnings 103 Updating the Firmware 105 Cleaning the Module 106 Safety Flap Flex Board 107 Transport Assembly Parts 108 Maintenance Functions 109 Simple Repairs 110 Exchanging the Needle Assembly 111 Exchanging the Needle Seat Assembly 114 Exchanging the Rotor Seal 116 Exchanging the Metering Seal and Piston 120 Exchanging the Gripper Arm 124 Exchanging the Interface Board 126 Replacing the Module s Firmware 127 Parts and Materials for Maintenance 129 Main Assemblies 130 Analytical Head Assembly 132 Vial Trays 134 Standard Autosampler Accessory Kit 136 Maintenance Kit 137 Injection Upgrade Kit 138 Multi Draw Kit 139 External Tray 140 Agilent 1260 Infinity Standard Autosampler User Manual Contents 10 Identifying Cables 141 Cable Overview 142 Analog Cables 144 Remote Cables 146 BCD Cables 149 External Contact Cable 151 CAN LAN Cables 152 Auxiliary Cable 153 RS 232 Cables 154 1 Hardware Information 155 Firmware
10. when handling electronic boards and components Switch OFF the autosampler at the main power switch Disconnect cables from the interface board connectors Loosen the screws Slide out the interface board from the autosampler Install the interface board Secure the screws ao A Q N Reconnect the cables to the board connectors Screws 126 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance 8 Replacing the Module s Firmware When Tools required OR OR Parts required Preparations The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if anew module with newer firmware is added to a system or if third party control software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Lab Advisor software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem ag
11. 1000 Pack 5182 0543 Crimp Top Vial 2 mL clear glass write on spot 100 Pack 5183 4492 Crimp Top Vial 2 mL clear glass write on spot 1000 Pack 5183 4494 Crimp Top Vial 2 mL clear glass write on spot 100 Pack silanized 5181 3376 Crimp Top Vial 2 mL amber glass write on spot 100 Pack 5183 4493 Crimp Top Vial 2 mL amber glass write on spot 1000 Pack 5183 4495 Crimp Top Vial 2 mL amber glass write on spot 100 Pack silanized 5182 0567 Crimp Top Vial 1 mL polypropylene wide opening 100 Pack 5183 4496 Crimp Top Vial 1 mL polypropylene wide opening 100 Pack silanized 9301 0978 crimp top vial 0 3 mL polypropylene wide opening 1000 pack Agilent 1260 Infinity Standard Autosampler User Manual Snap Top Vials p n 5182 0544 5183 4504 5183 4507 5182 0546 5183 4505 5183 4508 5182 0545 5183 4506 5183 4509 Screw Top Vials p n 5182 0714 5183 2067 5183 2070 5182 0715 5183 2068 5183 2071 5182 0716 5183 2069 5183 2072 Using the Autosampler 4 Description Snap Top Vial 2 mL clear glass 100 Pack Snap Top Vial 2 mL clear glass 1000 Pack Snap Top Vial 2 mL clear glass 100 Pack silanized Snap Top Vial 2 mL clear glass write on spot 100 Pack Snap Top Vial 2 mL clear glass write on spot 1000 Pack Snap Top Vial 2 mL clear glass write on spot 100 Pack silanized Snap Top Vial 2 mL amber glass write on spot 100 Pack Snap Top Vial 2 mL amber glass write on spot 1000 Pack
12. 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low Agilent 1260 Infinity Standard Autosampler User Manual 149 10 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module an 1 1 BCD 5 20 2 2 BCD7 80 Se 15 oO Z 3 3 BCD 6 40 oO So 4 4 BCD 4 10 s je 2 5 5 BCDO 1 fie e9 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 150 Agilent 1260 Infinity Standard Autosampler User Manual External Contact Ca ble BG 10 O15 oP at a 7 11 Identifying Cables One end of this cable provides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent Module Interface Board to general purposes 10 p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 Brown EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected Agilent 1260 Infinity Standard Autosampler User Manual 151 10 Identifying Cables CAN LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n Descripti
13. 40 humidity 25 l information on sound emission 184 injection sequence 13 injection valve 10 15 17 injection volume precision 51 injection volumes less than 2 ul 51 injection volumes 51 installing the autosampler flow connections 35 interface cables 32 power cable 32 safety 32 sample trays 40 installing the sample tray 37 installing the thermostatted autosampler safety 32 instrument layout 173 interfaces 159 internet 185 introduction to the autosampler 10 L LAN cable 152 leak sensor open 84 leak sensor short 85 leak 84 line frequency 25 line voltage 25 lithium batteries 180 lost CAN partner 83 low volume injections 51 Agilent 1260 Infinity Standard Autosampler User Manual Index M mainpass 13 maintenance functions change metering seal 62 change needle 60 step commands 66 maintenance definition of 102 feedback 172 replacing firmware 127 127 manual control 66 message ignition without cover 87 87 remote timeout 83 metering device 15 51 multi draw option 10 needle drive 15 16 needle into sample 66 needle into seat 67 needle up 66 66 Needle Seat Assembly Video Clip on page 124 61 non operating altitude 25 non operating temperature 25 numbering of vials 40 0 operating Altitude 25 operating temperature 25 optimizing performance automated needle wash 51 delay volume 51 delay volume adjustment 51 inject valve seal 51 low volume capillary kit 51 maintenance 51 minimizin
14. Agilent 1260 Infinity Standard Autosampler User Manual Error Information 7 Invalid Vial Position Error ID 4042 The vial position defined in the method or sequence does not exist The reflection sensors on the transport assembly flex board are used to automatically check which sample trays are installed coding on tray If the vial position does not exist in the current sample tray configuration the error message is generated Probable cause Suggested actions 1 Incorrect tray or trays installed Install the correct trays or edit the method or sequence accordingly 2 Incorrect vial positions defined in the Check method settings vs installed tray method or sequence 3 Tray recognition defective dirty sample tray Ensure the coding surfaces of the sample tray or defective transport assembly flex board are clean located at the rear of the sample tray If this does not solve the problem replace the transport assembly Agilent 1260 Infinity Standard Autosampler User Manual 99 7 Error Information Autosampler Error Messages 100 Agilent 1260 Infinity Standard Autosampler User Manual Agilent 1260 Infinity Standard Autosampler User Manual 8 Maintenance Introduction to Maintenance 102 Cautions and Warnings 103 Updating the Firmware 105 Cleaning the Module 106 Safety Flap Flex Board 107 Transport Assembly Parts 108 Maintenance Functions 109 Simple Repairs 110 Exchanging the Needle Assembly 111 Exchanging the
15. Agilent module interface board to general purposes RS 232 cables p n Description G1530 60600 RS 232 cable 2 m RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m Agilent 1260 Infinity Standard Autosampler User Manual 143 10 Identifying Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module a Shield Shield Analog Center Center Analog 144 Agilent 1260 Infinity Standard Autosampler User Manual Agilent Module to General Purpose Identifying Cables 10 p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog 3 Red Analog S e Agilent 1260 Infinity Standard Autosampler User Manual 145 10 Identifying Cables Remote Cables 146 One end of these cables provides a
16. Contact Cable 151 CAN LAN Cables 152 Auxiliary Cable 153 RS 232 Cables 154 Ee Agilent Technologies 141 10 Identifying Cables Cable Overview 142 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n 35900 60750 35900 60750 01046 60105 Remote cables p n 03394 60600 03396 61010 5061 3378 01046 60201 BCD cables p n 03396 60560 G1351 81600 Description Agilent module to 3394 6 integrators Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description Agilent module to 3396A Series integrators 3396 Series II 3395A integrator see details in section Remote Cables on page 146 Agilent module to 3396 Series III 3395B integrators Remote Cable Agilent module to general purpose Description Agilent module to 3396 integrators Agilent module to general purpose Agilent 1260 Infinity Standard Autosampler User Manual Identifying Cables 10 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n Description G1103 61611 External contact cable
17. Description 156 Interfaces 159 Setting the 8 bit Configuration Switch 166 Electrical Connections 170 Early Maintenance Feedback EMF 172 Instrument Layout 173 12 Appendix 175 General Safety Information 176 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 179 Lithium Batteries Information 180 Radio Interference 181 Solvent Information 182 Sound Emission 184 Agilent Technologies on Internet 185 Agilent 1260 Infinity Standard Autosampler User Manual Contents 8 Agilent 1260 Infinity Standard Autosampler User Manual a Agilent 1260 Infinity Standard Autosampler User Manual amp a e e e ee 1 7 e Introduction to the Autosampler ALS amp Introduction to the Autosampler ALS 10 Sampling Sequence 12 Sampling Unit 15 Transport Assembly 18 This chapter gives an introduction to the autosampler and instrument overview ee Agilent Technologies 1 ipoden to ihe Autosamplar A Introduction to the Autosampler ALS Gripper arm Needle seat Vial tray Switching valve 10 Analytical head 1 2 ks Figure 1 Overview of the Autosampler The Agilent 1260 Infinity autosampler is designed for use with other modules of the Agilent 1200 Infinity Series 1200 Series and 1100 Series LC or with other LC systems if adequate remote control inputs and outputs are available The autosamplers
18. View 30 Agilent 1260 Infinity Standard Autosampler User Manual Installing the Autosampler 3 Optimizing the Stack Configuration HN u Instant Pilot ST Detector Column compartment Solvent cabinet Autosampler Pump LAN to control software CAN bus cable to Instant Pilot Analog signal to recorder pmm an CAN bus cable AC Power Figure 11 Recommended Stack Configuration for a thermostatted ALS Rear View Agilent 1260 Infinity Standard Autosampler User Manual 31 3 Installing the Autosampler Installing the Autosampler Parts required Software required Preparations Description 1 Sampler 1 Power cord Agilent Data System and or Instant Pilot G4208A Locate bench space Provide power connection Unpack the Sampler Module is partially energized when switched off as long as the power cord is plugged in Risk of stroke and other personal injury Repair work at the module can lead to personal injuries e g shock hazard when the module cover is opened and the instrument is connected to power gt Never perform any adjustment maintenance or repair of the module with the top cover removed and with the power cord plugged in gt The security lever at the power input socket prevents that the module cover is taken off when line power is still connected Never plug the power line back in when cover is removed Personal injury To avoid personal injury keep fingers away f
19. a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to coul
20. are controlled by the Agilent Instant Pilot G4208A or by Agilent control software OpenLAB CDS ChemStation for LC EZChrom Elite etc Three sample rack sizes are available for the autosampler The standard full size rack holds 100 x 1 8 mL vials while the two half size racks provide space for 40 x 1 8 mL vials and 15 x 6 mL vials respectively Any two half size rack trays can be installed in the autosampler simultaneously A specially designed sample rack holding 100 x 1 8 mL vials is available for use with thermostatted autosamplers The half size racks trays are not designed for an optimal heat transfer when they are used with a thermostatted autosampler Agilent 1260 Infinity Standard Autosampler User Manual Introduction to the Autosampler ALS 1 The autosampler s transport mechanism uses an X Z Theta movement to optimize vial pick up and return A gripper arm picks up the vials and positions them below the sampling unit The gripper transport mechanism and sampling unit are driven by motors Movement is monitored by optical sensors and optical encoders to ensure correct operation The metering device is always flushed after injection to ensure minimum carry over The standard analytical head of the metering device provides injection volumes from 0 1 100 uL and can be operated at up to 600 bar A head with extended volume is available for injection volumes from 0 1 900 uL and can be operated up to 400 bar G1329B or 200 bar G1329A
21. cover must be in place when Start and End are selected Start Moves the safety flap away from the needle and positions the needle approximately 15 mm above the needle seat Needle Up Press function key couple of times to move the needle arm up in 2 mm steps Needle Down Press function key couple of times to move the needle arm down in 2 mm steps The lowest position end position is used to align the needle at the correct position in the needle seat End Completes the procedure by moving the gripper arm to the home position and releasing the safety flap Agilent 1260 Infinity Standard Autosampler User Manual Troubleshooting and Diagnostics 6 Using the Change Needle Function 1 Ensure the front cover is installed 2 Select Start to move the needle arm to the maintenance position 3 Remove the front cover Do not remove the front cover until the needle arm is in its maintenance position Removing the cover while the needle arm is activated may lock up the system 4 Exchange the needle or needle seat see Exchanging the Needle Seat Assembly on page 114 and Exchanging the Needle Assembly on page 111 5 Replace the front cover Select End to complete the procedure Agilent 1260 Infinity Standard Autosampler User Manual 61 6 Troubleshooting and Diagnostics Change Piston The change piston function draws the piston away from the home position relieving the tension on the spring In this position
22. label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information Agilent 1260 Infinity Standard Autosampler User Manual 179 12 Appendix Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren 180 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien m
23. leak sensor is thoroughly dry before restarting the autosampler Leak Sensor Open Error ID 0083 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the main Please contact your Agilent service board representative 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative Agilent 1260 Infinity Standard Autosampler User Manual Error Information 7 Leak Sensor Short Error ID 0082 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Please contact your Agilent service representative 2 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative Compensation Sensor Open Error ID
24. of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 184 Agilent 1260 Infinity Standard Autosampler User Manual Appendix 12 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Agilent 1260 Infinity Standard Autosampler User Manual 185 Index Index 8 bit configuration switch without On Board LAN 166 A Agilent Lab Advisor software 77 Agilent Lab Advisor 77 Agilent on internet 185 air circulation 24 algae information 182 ambient non operating temperature 25 ambient operating temperature 25 analog signal 163 analog cable 144 analytical head 16 apg remote 164 auxiliary cable 153 B battery safety information 180 BCD cable 149 bench space 24 24 bypass 13 C cable analog 144 auxiliary 153 186 BCD 149 CAN 152 connecting APG remote connecting CAN 30 31 connecting LAN 30 31 connecting the ChemStation 30 31 30 31 connecting the power 30 31 external contact 151 LAN 152 remote 146 RS 232 154 cables analog 142 BCD 142 CAN 143 external contact 143 LAN 143 overview 142 remote 142 RS 232 143 CAN cable 152 capillaries 35 caps crimp 44 screw 44 snap 44 cautions and warnings 103 change meterin
25. or shipping the autosampler Unsecured Transportation of Autosampler Unsecured transportation of the autosampler may result in mechanical damage to the gripper and transport slider gt Always secure the arm in the park position 64 Before parking the gripper arm ensure there is no vial in the gripper Use the Release Gripper function to remove the vial 1 Select Park Arm 2 When the arm is in the park position the autosampler is ready for shipment and can be switched OFF Agilent 1260 Infinity Standard Autosampler User Manual Troubleshooting and Diagnostics 6 Change Gripper The change gripper function moves the gripper to the front of the autosampler enabling easy access to the gripper release mechanism User Interface The commands for the control software are Start Moves the transport assembly and gripper arm to the position required to change the gripper arm End Repositions the transport assembly and gripper arm to the home position Using the Change Seal Function 1 Select Start to move the gripper arm to the maintenance position 2 Exchange the gripper arm see Exchanging the Gripper Arm on page 124 3 Select End to move the gripper arm to the home position Tray Alignment Tray alignment is required to compensate for small deviations in positioning of the gripper which may occur after disassembling the module for repair The tray alignment procedure uses several tray positions as refe
26. program includes the command NEEDLE WASH When this command is included in the injector program the needle is lowered once into the specified wash vial before injection For example 1 DRAW 5 ul 2 NEEDLE WASH vial 7 3 INJECT Line 1 draws 5 ul from the current sample vial Line 2 moves the needle to vial 7 Line 3 injects the sample valve switches to main pass Using an Injector Program The process is based on a program that switches the bypass grove of the injection valve into the flow line for cleaning This switching event is performed at the end of the equilibration time to ensure that the bypass grove is filled with the start concentration of the mobile phase Otherwise the separation could be influenced especially if microbore columns are used For example Outside wash of needle in vial 7 before injection Injector program Draw x x y ul from sample NEEDLE WASH vial 7 Inject Wait equilibration time see text above Valve bypass Wait 0 2 min Valve mainpass Valve bypass Valve mainpass Overlapped injection together with additional injection valve switching is not possible Agilent 1260 Infinity Standard Autosampler User Manual 47 5 Optimizing Performance General Recommendation to Lowest Carry over e For samples where needle outside cannot be cleaned sufficiently with water or alcohol use wash vials with an appropriate solvent Using an injector program and several wash vials can be used for cleani
27. step functions 56 stepper motor 16 T temperature sensor 84 temperature 26 theta axis 18 timeout 82 transport assembly 18 transport mechanism 10 transport 63 tray alignment 65 troubleshooting error messages 56 81 status indicators 56 U upgrade kit injection 138 V valve bypass 66 valve capillaries 35 valve mainpass 67 vial contents temperature 26 vial numbering 40 vial racks 10 vial to seat 66 vial to tray 66 vials 10 screwtop 43 snaptop 43 top crimp 42 viscous samples 51 51 voltage range 25 volume 38 Agilent 1260 Infinity Standard Autosampler User Manual Ww warnings and cautions 103 waste electrical and electronic equipment 179 WEEE directive 179 weight 24 25 X X axis 18 Z Z axis 18 Agilent 1260 Infinity Standard Autosampler User Manual Index 189 www agilent com In This Book This manual contains user information about the Agilent 1260 Infinity Standard Autosampler G1329B The manual describes the following e introduction to the autosampler site requirements and specifications installing the autosampler e using the autosampler optimizing performance troubleshooting and diagnostics e maintenance parts and materials cable overview e safety and warranty Agilent Technologies 2007 2012 2013 Printed in Germany 04 2013 G1329 90015 Rev B fe Agilent Technologies
28. sure the power cords are unplugged before disconnecting or reconnecting the sampler to thermostat cable Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only 22 Agilent 1260 Infinity Standard Autosampler User Manual CAUTION Site Requirements and Specifications 2 Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using c
29. than 50 C 122 F Agilent 1260 Infinity Standard Autosampler User Manual 25 2 Site Requirements and Specifications Performance Specifications Standard Autosampler 26 Table 4 Performance Specifications Agilent 1260 Infinity Standard Autosampler G1329B Type Specification Pressure Operating range 0 60 MPa 0 600 bar 0 8850 psi GLP features Early maintenance feedback EMF electronic records of maintenance and Communications Safety features Injection range Replicate injections Precision Minimum sample volume Carryover Sample viscosity range Sample capacity Injection cycle time Metering device errors Controller area network CAN RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 1 100 uL in 0 1 pL increments recommended 1 L increments Up to 1500 uL with multiple draw hardware modification required 1 99 from one vial Typically lt 0 25 RSD of peak areas from lt 5 pL to 100 pL Typically lt 1 RSD of peak areas from 1 uL to 5 pL 1 pL from 5 pL sample in 100 pL microvial or 1 uL from 10 pL sample in 300 pL microvial Typically lt 0 1 lt 0 05 with external needle cleaning 0 2 50 cp 100 x 2 mL vials in 1 tray 40 x2 mL vials in tray 15 x 6 mL vials in tray Agilent vials only 50 s for d
30. to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e ared LED may indicate a problem of a different module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of main firmware A fast blinking indicator indicates that the module is in a low level error mode In such a case try to re boot the module or try a cold start see Special Settings on page 169 Then try a firmware update see Replacing the Module s Firmware on page 127 If this does not help a main board replacement is required Agilent 1260 Infinity Standard Autosampler User Manual Troubleshooting and Diagnostics 6 Maintenance Functions Certain maintenance procedures require the needle arm metering device and gripper assembly to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the approp
31. to the upper position Command also positions the vial at the seat and lifts the needle to the upper position Command also positions the vial at the seat lifts the needle and lowers the needle into vial Command can be done more than once maximum draw volume of 100 uL cannot be exceeded Use Plunger Home to reset the metering device Command also switches the valve to bypass if it is not already in that position Command also lifts the needle to the upper position Agilent 1260 Infinity Standard Autosampler User Manual Troubleshooting and Diagnostics 6 Table 6 Injector step commands Step Action Comments Needle into Seat Lowers the needle arm into Command also returns the vial to the tray the seat position Valve Mainpass Switches the injection valve to the mainpass position Reset Resets the injector Agilent 1260 Infinity Standard Autosampler User Manual 67 Troubleshooting and Diagnostics Troubleshooting 68 If the autosampler is unable to perform a specific step due to a hardware failure an error message is generated You can use the injector steps to do the injection sequence while observing how the instrument responds Table 7 on page 68 summarizes the injector steps and lists the associated error messages and probable causes of step failures Table7 Step Failures Step Function Probable Failure Modes Bypass Valve already in bypass Valve not connected Defective injection valve Pis
32. 20 Introduction to the Autosampler ALS Introduction to the Autosampler ALS Agilent 1260 Infinity Standard Autosampler User Manual Agilent 1260 Infinity Standard Autosampler User Manual e00 ee 2 r Site Requirements and Specifications Site Requirements 22 Physical Specifications 25 Performance Specifications Standard Autosampler 26 This chapter provides information on environmental requirements physical and performance specifications ee Agilent Technologies 21 2 Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimum performance of the instrument Power Consideration CAUTION The autosampler power supply has wide ranging capability see Table 3 on page 25 Consequently there is no voltage selector in the rear of the autosampler There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply The thermostatted autosampler comprises two modules the standard autosampler and the thermostat G1330B Both modules have a separate power supply and a power plug for the line connections The two modules are connected by a control cable and both are turned on by the autosampler module Damaged electronics Disconnecting or reconnecting the sampler to thermostat cable when the power cords are connected to either of the two modules will damage the electronics of the modules gt Make
33. 7 Error Information Missing Wash Vial 98 Invalid Vial Position 99 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 80 Agilent 1260 Infinity Standard Autosampler User Manual Error Information 7 What are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in the user interface Special handling is done in case of a leak As a lea
34. Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low z NC 4 Blue Shut down Low e oa NC 5 Pink Not Ls connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready Agilent 1260 Infinity Standard Autosampler User Manual Identifying Cables 10 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground ae NC 2 Brown Prepare run Low o i 3 3 Gray Start Low z NC 4 Blue Shut down Low a NC 5 Pink Not Ls connected Se NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 Pin Agilent Signal Name Active
35. G4226 47200 3 G4226 43200 G1313 09101 4 G1329 60011 5 0570 1574 6 G1313 44513 7 G1313 44512 Parts and Materials for Maintenance Description Adapter air channel Tray base Plug Spring Thermostattable tray for 100 x 2 mL vials Spring stud Halftray for 15 x 6 mL vials Halftray for 40 x 2 mL vials Agilent 1260 Infinity Standard Autosampler User Manual 9 135 9 Parts and Materials for Maintenance Standard Autosampler Accessory Kit p n Description 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 5181 1519 CAN cable Agilent module to module 1 m 5959 3890 Label Halftray 9222 0518 Bag plastics 01090 87306 Capillary ST 0 17 mm x 380 nm S S Heat exchanger G1313 44101 3x Finger Cap G1329 40321 Door Front CA G1329 43200 Adapter air channel G1329 44111 Cover insulation G1329 90122 1200 Sampler Door Upgrade Tech Notes ENG 136 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance Kit G1329B PM Kit p n G1313 68719 0101 1416 G1313 87201 G1329 87017 G1329A PM Kit p n G1313 68730 0100 1853 61313 87201 G1329 87017 Seals and Caps p n 5063 6589 5063 6506 1 Reorder gives pack of 15 Parts and Materials for Maintenance 9 Description G1329B PM Kit including Rotor Seal Needle Seat Assy Metering Seal Rotor seal PEEK 2 grooves max 600 bar Needle assembly Needle seat assy 0 17 mm i d 2 3 uL Description G1329A PM Kit including Vespel R
36. Needle Seat Assembly 114 Exchanging the Rotor Seal 116 Exchanging the Metering Seal and Piston 120 Exchanging the Gripper Arm 124 Exchanging the Interface Board 126 Replacing the Module s Firmware 127 This chapter describes the maintenance of the module oh Agilent Technologies 101 8 Maintenance Introduction to Maintenance The module is designed for easy maintenance Maintenance can be done from the front with module in place in the system stack There are no serviceable parts inside Do not open the module 102 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance 8 Cautions and Warnings WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides WARNING Sharp metal edges Sharp edged parts of the equipment may cause injuries gt To prevent personal injury be careful when getting in contact with sharp metal areas WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When wo
37. Snap Top Vial 2 mL amber glass write on spot 100 Pack silanized Description Screw Cap Vials 2 mL clear glass 100 Pack Screw Top Vial 2 mL clear glass 1000 Pack Screw Top Vial 2 mL clear glass 100 Pack silanized Screw Top Vial 2 mL clear glass write on spot 100 Pack Screw Top Vial 2 mL clear glass write on spot 1000 Pack Screw Top Vial 2 mL clear glass write on spot 100 Pack silanized Screw Cap Vial 2 mL amber glass write on spot 100 pk Screw Top Vial 2 mL amber glass write on spot 1000 Pack Screw Top Vial 2 mL amber glass write on spot 100 Pack silanized Agilent 1260 Infinity Standard Autosampler User Manual 43 4 44 Using the Autosampler Crimp Caps p n 5181 1210 5183 4498 5181 1215 5181 1216 5181 1217 Snap Caps p n 5182 0550 5182 3458 5182 3457 5182 3459 Screw Caps p n 5182 0717 5182 0718 5182 0719 5182 0720 5182 0721 5182 0722 Description Crimp Cap silver aluminum septum clear PTFE red rubber 100 Pack Crimp Cap silver aluminum septum clear PTFE red rubber 1000 Pack Crimp Cap blue aluminum septum clear PTFE red rubber 100 Pack Crimp Cap green aluminum septum clear PTFE red rubber 100 Pack Crimp Cap red aluminum septum clear PTFE red rubber 100 Pack Description Snap Cap clear polypropylene septum clear PTFE red rubber 100 Pack Snap Cap blue polypropylene septum clear PTFE red rubber 100 Pack Snap Cap green polyp
38. X axis and clean them DO NOT lubricate the transport rod 2 Lubricate the X gear Friction can result in the belt slipping on the gear so that the position of the belt teeth towards the gear changes To avoid this apply some grease from the sample transport repair kit to the X motor gear Do not use other grease as the one in the kit and carefully follow the instruction from the technical note Agilent 1260 Infinity Standard Autosampler User Manual 73 74 Troubleshooting and Diagnostics 3 Check the tension of the belts For this use the ALS Torque Verification in LabAdvisor and measure the torque for theta and X axis Table 9 Typical ranges Theta both 30 50 X axis both 50 90 Ifthe theta or X torque is not in the range proceed with step 4 on page 74 if you think you can adjust the torque Otherwise proceed with step 6 on page 74 Adjust the belts tension If the measured torque value is too low the belt needs to be tightened If the measured torque value is too high the belt needs to be loosened For this slide the motor X or theta on the holder bracket in the appropriate direction and test the tension with the ALS Torque Verification in LabAdvisor Repeat this steps until the values are in the appropriate torque range Reset the sampler alignment to default value Reset tray alignment and transport alignment is possible with the Control Module and the ChemStation To reset the transport al
39. aar inleveren als KCA Agilent 1260 Infinity Standard Autosampler User Manual Appendix 12 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Agilent 1260 Infinity Standard Autosampler User Manual 181 12 Appendix Solvent Information 182 Observe the following recommendations on the use of solvents Flow Cell Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell Prevent any crystallization of buffer solutions This will lead into a blockage damage of the flow cell If the flow cell is transported while temperatures are below 5 C it must be assured that the cell is filled with alcohol Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add small of organic solvents e g Acetonitrile or Methanol 5 Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive s
40. ables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Agilent 1260 Infinity Standard Autosampler User Manual 23 2 Site Requirements and Specifications WARNING Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Bench Space The autosampler can be placed on almost any laboratory bench for dimensions and weight see Table 3 on page 25 The instrument requires an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 3 1 inch at the rear for the circulation of air and room for electrical connections Ensure the autosampler is installed in a horizontal position If a complete Agilent 1200 Infinity Series LC is to be installed on the bench make sure that the bench is designed to carry the weight of all the modules For a complete system including the thermostatted autosampler it is recommended to position the modules in two stacks see Optimizing the Stack Configuration on page 29 Make sure that in this configuration there is 25 cm 10 inch space on either side of the thermostatted autosample
41. al Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces There is no special interface for this module Agilent 1260 Infinity Standard Autosampler User Manual 165 11 Hardware Information Setting the 8 bit Configuration Switch 166 The 8 bit configuration switch is located at the rear of the module This module does not have its own on board LAN interface It can be controlled through the LAN interface of another module and a CAN connection to that module Figure 22 Configuration switch settings depend on c
42. at head screwdriver to ease out the needle seat 114 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance 8 3 Insert the new needle seat assembly Press the seat firmly into position Sy I gt I 7 J PN 4 Connect the seat capillary fitting to port 5 of the injection valve Dq WG j A 5 Use Down to position the needle approximately 2 mm above the seat 6 Ensure the needle is aligned with the seat If required bend the needle slightly until the needle is aligned correctly Next Steps 7 On completion of this procedure Install the front cover 8 Select End in the maintenance function Change Needle see Change Needle on page 60 Agilent 1260 Infinity Standard Autosampler User Manual 115 8 Maintenance Exchanging the Rotor Seal When Poor injection volume reproducibility Leaking injection valve Tools required Description Wrench 1 4 inch supplied in HPLC Tool Kit Hexagonal key 9 64 inch supplied in HPLC Tool Kit Parts required p n Description 1 0100 1853 Rotor seal Vespel 1 0100 1849 Rotor seal Tefzel 1 0101 1416 Rotor seal PEEK Preparations Remove front cover Remove the leak tubing if necessary CAUTION Removing the stator head The stator face is held in place by the stator head When you remove the stator head the stator face can fall out of the valve gt Carefully handle the valve to prev
43. cal note Agilent 1260 Infinity Standard Autosampler User Manual 75 76 Troubleshooting and Diagnostics 3 Check the tension of the belts For this use the ALS Torque Verification in LabAdvisor and measure the torque for each axis Table 10 Typical ranges Theta both 30 50 X axis both 50 90 Z axis both 90 130 Gripper open 30 65 Gripper closed maximum 30 4 Exchange Gripper assembly G1313 60010 For exchange procedure see Exchanging the Gripper Arm on page 124 5 If these measures don t resolve the problem it is necessary to exchange the transport assembly or the main board To do this please contact your Agilent service representative Agilent 1260 Infinity Standard Autosampler User Manual Troubleshooting and Diagnostics 6 Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Fee
44. ced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere 1 Connect the pump outlet capillary to port 1 of the injection valve 2 Connect column compartment inlet capillary to port 6 of the injection valve Connect the corrugated waste tube to the solvent waste from the leak plane 4 Ensure that the waste tube is positioned inside the leak channel Do not extend the waste capillary of the autosampler The siphoning effect might empty the complete seat capillary introducing air into the system Agilent 1260 Infinity Standard Autosampler User Manual 35 3 Installing the Autosampler Flow Connections Column compartment inlet capillary Pump outlet capillary Solvent waste for corrugated tube Waste tub in leak channel Figure 13 Hydraulic Connections 36 Agilent 1260 Infinity Standard Autosampler User Manual Installing the Autosampler Installing the Sample Tray 1 Open the front door 2 Load the sample tray with sample vials as required Slide the sample tray into the autosampler so that the rear of the sample tray is seated firmly against the rear of the sample tray area 4 Press the front of the sample tray down to secure the tray in the autosampler If the thermostatted autosampler tray pops out of position the air channel adapter is not inserted correctly Figure 14 Installing the Sample Tray Agilent 1260 Infini
45. ction mode Increase of draw and eject speed for large injection volumes e Add at last run a blank if overlapped injection is used To reduce the injection time the detector balance has to be set to OFF Agilent 1260 Infinity Standard Autosampler User Manual Optimizing Performance 5 Precise Injection Volume Injection Volumes Less Than 2 pL When the injection valve switches to the BYPASS position the mobile phase in the sample loop is depressurized When the syringe begins drawing sample the pressureof the mobile phase is decreased further If the mobile phase is not degassed adequately small gas bubbles may form in the sample loop during the injection sequence When using injection volumes lt 2 uL these gas bubbles may affect the injection volume precision For best injection volume precision with injection volumes lt 2 uL use of an Agilent 1260 Infinity degasser is recommended to ensure the mobile phase is adequately degassed Also using the automated needle wash see Optimization for Lowest Carry over on page 46 between injections reduces carry over to a minimum further improving the injection volume precision Draw and Eject Speed Draw Speed The speed at which the metering unit draws sample out of the vial may have an influence on the injection volume precision when using viscous samples If the draw speed is too high air bubbles may form in the sample plug affecting precision The default draw speed is 200
46. d result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Agilent 1260 Infinity Standard Autosampler User Manual In This Book In This Book This manual covers the Agilent 1260 Infinity Standard Autosampler G1329B 1 Introduction to the Autosampler ALS This chapter gives an introduction to the autosampler and instrument overview 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Autosampler This chapter provides information on unpacking checking on completeness stack considerations and installation of the module 4 Using the Autosampler This chapter provides information on how to set up the module for an analysis and explains the basic settings 5 Optimizing Performance This chapter provides information on how to optimize the module 6 Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic features 7 Error Information This chapter describes the meaning of erro
47. d in any combination enabling both 2 mL and 6 mL vials to be used simultaneously Numbering of Vial Positions The standard 100 vial tray has vial positions 1 to 100 However when using two half trays the numbering convention is slightly different The vial positions of the right hand half tray begin at position 101 as follows Left hand 40 position tray 1 40 Left hand 15 position tray 1 15 Right hand 40 position tray 101 140 Right hand 15 position tray 101 115 Agilent 1260 Infinity Standard Autosampler User Manual Using the Autosampler 4 Position 1 Left hand half tray y Position 101 Right hand half tray oS 5 Figure 15 Numbering of Tray Positions Agilent 1260 Infinity Standard Autosampler User Manual 41 4 Using the Autosampler Choice of Vials and Caps 42 List of Compatible Vials and Caps For reliable operation vials used with the autosampler must not have tapered shoulders or caps that are wider than the body of the vial The vials in Crimp Top Vials on page 42 Snap Top Vials on page 43 and Screw Top Vials on page 43 and caps in Crimp Caps on page 44 Snap Caps on page 44 and Screw Caps on page 44 shown with their Part numbers have been successfully tested using a minimum of 15 000 injections with the autosampler Crimp Top Vials p n Description 5181 3375 Crimp Top Vial 2 mL clear glass 100 Pack 5183 4491 Crimp Top Vial 2 mL clear glass
48. d into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself Agilent 1260 Infinity Standard Autosampler User Manual 173 11 Hardware Information Instrument Layout 174 Agilent 1260 Infinity Standard Autosampler User Manual Agilent 1260 Infinity Standard Autosampler User Manual 12 Appendix General Safety Information 176 General Safety Information 176 Safety Standards 176 Operation 177 Safety Symbols 178 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 179 Lithium Batteries Information 180 Radio Interference 181 Solvent Information 182 Sound Emission 184 Agilent Technologies on Internet 185 This chapter provides addition information on safety legal and web ee Agilent Technologies 175 12 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liab
49. dard valve only For mobile phases with pH below 2 3 or above 9 5 or for conditions where the lifetime of the Vespel seal is drastically reduced a seal made of Tefzel is available Tefzel is more resistant than Vespel to extremes of pH however is a slightly softer material Under normal conditions the expected lifetime of the Tefzel seal is shorter than the Vespel seal however Tefzel may have the longer lifetime under more extreme mobile phase conditions PEEK Seal for preparative injection valve only The preparative injection valve has a sealing material made of PEEK This material has high chemical resistance and versatility It is suitable for application using mobile phases within a pH between 1 and 14 This seal is also used for the G1329B module Strong oxidizing acids such as concentrated nitric and sulfuric acids are not compatible with PEEK Agilent 1260 Infinity Standard Autosampler User Manual 53 5 Optimizing Performance Choice of Rotor Seal 54 Agilent 1260 Infinity Standard Autosampler User Manual Agilent 1260 Infinity Standard Autosampler User Manual 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions 56 Status Indicators 57 Power Supply Indicator 57 Module Status Indicator 58 Maintenance Functions 59 User Interface 59 Change Needle 60 Change Piston 62 Park Arm 63 Change Gripper 65 Tray Alignment 65 ALS Step Commands 66 Troubleshooting 68 Troubleshoot
50. dback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files The Instrument Utilities is a basic version of the Lab Advisor with limited functionality required for installation use and maintenance No advanced repair troubleshooting and monitoring functionality is included Agilent 1260 Infinity Standard Autosampler User Manual 77 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 78 Agilent 1260 Infinity Standard Autosampler User Manual Agilent 1260 Infinity Standard Autosampler User Manual 7 Error Information What are Error Messages 81 General Error Messages 82 Timeout 82 Shutdown 82 Remote Timeout 83 Lost CAN Partner 83 Leak 84 Leak Sensor Open 84 Leak Sensor Short 85 Compensation Sensor Open 85 Compensation Sensor Short 86 Fan Failed 86 OpenCover 87 Restart Without Cover 87 Autosampler Error Messages 88 Frontdooropen 88 Arm Movement Failed 89 Valve to Bypass Failed 90 Valve to Mainpass Failed 90 Needle Up Failed 91 Needle Down Failed 92 Missing Vial 93 Initialization Failed 94 Metering Home Failed 95 Motor Temperature 96 Initialization with Vial 97 Safety Flap Missing 97 Vial in Gripper 98 oe Agilent Technologies 79
51. e are no other field replaceable parts in the transport assembly If any other component is defective flex board spindles plastic parts the complete unit must be exchanged 108 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance 8 Maintenance Functions Certain maintenance procedures require the needle arm metering device and gripper assembly to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position For details refer to Maintenance Functions on page 59 Agilent 1260 Infinity Standard Autosampler User Manual 109 8 Maintenance Simple Repairs The procedures described in this section can be done with the autosampler in place in the stack You will do some of these procedures on a more frequent basis Table 11 Maintenance Procedures Procedure Typical Frequency Time Required Notes Exchanging the needle assembly Exchanging the seat assembly Exchanging the rotor seal Exchanging the metering seal Exchanging the gripper arm When needle shows indication of damage or blockage When the seat shows indication of damage or blockage After approximately 30000 to 40000 injections or when the valve performance shows indication of leakage or wear When autosampler reproducibility indicates seal wear When the gripper arm is defective 15 minutes 10 minutes 30 minutes 30 minute
52. e unobstructed movement of the transport assembly 2 High friction in the transport assembly Please contact your Agilent service representative 3 Defective motor assembly Please contact your Agilent service representative 4 Defective sample transport assembly flex Please contact your Agilent service board representative 5 Defective main board Please contact your Agilent service representative Agilent 1260 Infinity Standard Autosampler User Manual 89 7 Error Information Valve to Bypass Failed Error ID 4014 4701 The injection valve failed to switch to the bypass position The switching of the injection valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the bypass position or if the microswitch does not close the error message is generated Probable cause Suggested actions 1 Defective injection valve Please contact your Agilent service representative 2 Defective main board Please contact your Agilent service representative Valve to Mainpass Failed Error ID 4015 The injection valve failed to switch to the mainpass position The switching of the injection valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the mainpass position or if the microswitch does not close the error message is generated
53. efore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled Agilent 1260 Infinity Standard Autosampler User Manual Hardware Information 11 Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molde
54. ent damage to the stator face 116 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance 8 Exchanging the Rotor Seal 1 Remove all capillary fittings from the injection valve ports 2 Loosen each fixing bolt two turns at a time Remove the bolts from the head WF 59X n j y AEE 4 Remove the rotor seal and isolation seal rauaga Agilent 1260 Infinity Standard Autosampler User Manual 117 Maintenance 5 Install the new rotor seal and isolation seal Ensure the metal spring inside the isolation seal faces towards the valve body 6 Install the stator ring with the short of the two pins facing towards you at the 12 O Clock position Ensure the ring sits flat on the valve body 7 Install stator head Tighten the bolts alternately two turns 8 Reconnect the pump capillaries to the valve ports see at a time until the stator head is secure Hydraulic Connections Dy e q J Th TNS N 118 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance Exchanging the Rotor Seal 9 Slide the waste tube into the waste holder in the leak tray N No RY SQ ENSALNI LN y 4 pei eK f D TS th o D ly Wt 10 On completion of this procedure Install the front cover Agilent 1260 Infinity Standard Autosampler User Manual 119 8 Maintenance Exchangin
55. er to store the values in the non volatile memory Table 16 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 Rs 232c 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up Agilent 1260 Infinity Standard Autosampler User Manual 167 11 Hardware Information 168 Table 17 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table 18 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication Table 19 Parity Settings without on board LAN Switches Parity 7 e 0 0 No Parity 0 1 Odd Parity 1 1 Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity Agilent 1260 Infinity Standard Autosampler User Manual Hardware Information 11 Special Settings The special settings are required for specific actions normally in a service case Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the fol
56. eries 10 Turn ON power by pushing the button at the lower left hand side of the sampler lal number output E z L E i i E Gaw OEE Remote RS232C ap CAN bus oh Oe e Relay contacts a en olf Figure 12 Cable Connections Agilent 1260 Infinity Standard Autosampler User Manual 33 3 Installing the Autosampler Installing the Autosampler If the front cover is not installed the autosampler is in a not ready condition and operation is NOTE inhibited NOTE The sampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OFF 34 Agilent 1260 Infinity Standard Autosampler User Manual Installing the Autosampler 3 Flow Connections Tools required Parts required Preparations Description Parts from the HPLC Tool Kit Description Parts from the Accessory kit Sampler is installed in the LC system Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be redu
57. feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 Agilent 1260 Infinity Standard Autosampler User Manual Hardware Information Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table 12 Agilent 1200 Infinity Series Interfaces 11 Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Pumps G1310B Iso Pump 2 Yes No Yes 1 Yes G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump G4220A B Bin Pump 2 No Yes Yes No Yes CAN DC OUT for CAN G4204A Quat Pump slaves G1361A Prep Pump 2 Yes No Yes No Yes CAN DC OUT for CAN slaves Samplers G1329B ALS 2 Yes No Yes No Yes THERMOSTAT for G2260A Prep ALS G1330B K1330B G1364B FC PS 2 Yes No Yes No Yes THERMOSTAT for G1364C FC AS G1330B K1330B G1364D FC uS CAN DC OUT for CAN G1367E HiP ALS slaves K1367E HiP ALS Clinical Ed G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert
58. fety flap not in position the error message is generated Probable cause Suggested actions 1 Safety flap missing or broken Please contact your Agilent service representative Agilent 1260 Infinity Standard Autosampler User Manual 97 7 98 Error Information Vial in Gripper Error ID 4033 The gripper arm attempted to move with a vial still in the gripper During specific stages of the sampling sequence no vial should be held by the gripper The autosampler checks if a sample vial is stuck in the gripper by closing and opening the gripper fingers while monitoring the motor encoder If the gripper fingers are unable to close the error message is generated Probable cause Suggested actions 1 Vial still in gripper Remove the vial using the Release Vial function in the user interface Reinitialize the autosampler Missing Wash Vial Error ID 4035 4542 4707 The wash vial programmed in the method was not found When the gripper arm picks a vial out of the sample tray the processor monitors the gripper motor encoder If a vial is present the closing of the gripper fingers is limited by the vial However if no vial is present the gripper fingers close too far This is sensed by the processor encoder position causing the error message to be generated Probable cause Suggested actions 1 No wash vial in the position defined in the Install the wash vial in the correct position or method edit the method accordingly
59. g delay volume 38 Index P packaging damaged 28 park arm 63 parts and materials analytical head assembly 100 microlitre 132 analytical head assembly optional 900 microlitre 133 analytical head assembly 132 133 external tray 140 main assemblies 130 multi draw kit 139 vial trays and tray base 134 parts damaged 28 missing 28 performance specifications autosampler 26 physical specifications 25 plungerhome 66 Power Consideration 22 power consumption 25 power cords 23 power requirements 22 power supply indicator 5 7 preparative head 16 R radio interference 181 remote cable 146 repairs cautions and warnings 103 metering piston 120 metering seal 120 needle assembly 111 needle seat assembly 114 replacing firmware 127 127 188 rotorseal 116 simple repairs 110 reset 6 7 restart without cover 87 RS 232C cable 154 communication settings 167 S safety class 176 safety information lithium batteries 180 safety general information 176 standards 25 symbols 178 sample trays 40 numbering of vial positions 40 sampling sequence 12 sampling unit 15 serial number information 171 shipping 63 shutdown 82 site requirements 22 power cords 23 sound emission 184 special interfaces 165 special settings boot resident 169 forced cold start 169 specification physical 25 specifications 26 stack configuration 30 31 rearview 30 31 stator 17 status indicators 57 status indicator 58 step commands 66
60. g seal 62 change needle 60 choice of vials and caps 51 cleaning 106 Communication settings RS 232C 167 compensation sensor open 85 compensation sensor short 86 condensation 24 D defect on arrival 28 delay volume 38 delay 38 delivery checklist 28 dimensions 25 draw speed 51 51 draw 51 66 E eject speed 51 51 EJECT 51 electrical connections descriptions of 1 0 electronic waste 1 9 electrostatic discharge ESD 126 EMF early maintenance feedback 172 environment 22 error messages arm movement failed 89 compensation sensor open 85 compensation sensor short 86 fan failed 86 ignition without cover 87 87 initialization failed 94 initialization with vial 97 invalid vial position 99 eak sensor open 84 eak sensor short 85 eak 84 Agilent 1260 Infinity Standard Autosampler User Manual lost CAN partner 83 metering home failed 95 missing vial 93 missing wash vial 98 motor failed 96 needle down failed 92 needle up failed 91 remote timeout 83 safety flap missing 97 shutdown 82 timeout 82 valve to bypass failed 90 valve to mainpass failed 90 vial in gripper 98 external contact cable 151 F fan failed 86 firmware description 156 main system 156 resident system 156 update tool 157 updates 157 127 127 upgade downgrade 127 upgrade downgrade 127 flow connections 35 frequency range 25 fuses 22 G gripper alignment 65 gripper fingers 18 gripper alignment 56 external vials 56 H half trays
61. g the Metering Seal and Piston When Poor injection volume reproducibility Leaking metering device Tools required p n Description Wrench 1 4 inch supplied in HPLC Tool Kit Hexagonal key 4 mm supplied in HPLC Tool Kit 8710 2411 Hexagonal key 3 mm supplied in HPLC Tool Kit Parts required p n Description 1 5063 6589 Metering seal pack of 2 for 100 uL analytical head 1 0905 1294 Metering seal pack of 1 for 900 uL analytical head 1 5063 6586 Sapphire piston only if scratched or contaminated 1 5062 8587 Metering piston for 900 uL analytical head only if scratched or contaminated Preparations Select Start in the maintenance function Change piston see Change Piston on page 62 Remove the front cover 120 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance 1 Remove the two capillaries from the metering head assembly 2 Remove the two fixing bolts and pull the head assembly away from the sampler Notice that the closed side of the metering head faces upwards 3 Remove the two fixing bolts from the base of the metering head assembly 4 Disassemble the metering head assembly Agilent 1260 Infinity Standard Autosampler User Manual 121 Maintenance 5 Use a small screwdriver to carefully remove the seal Clean the chamber with lint free cloth Ensure all particular matter is removed 6 Install the new seal Press the seal firmly into position 7 P
62. gn ensures that sample loop needle inside seat capillary and the mainpass of the injection valve is always in the flow line These parts are continuously flushed during an isocratic and also during a gradient analysis The residual amount of sample remaining on the outside of the needle after injection may contribute to carry over in some instances When using small injection volumes or when injecting samples of low concentration immediately after samples of high concentration carry over may become noticeable Using the automated needle wash enables the carry over to be minimized and prevents also contamination of the needle seat Using the Automated Needle Wash The automated needle wash can be programmed either as injection with needle wash or the needle wash can be included into the injector program When the automated needle wash is used the needle is moved into a wash vial after the sample is drawn By washing the needle after drawing a sample the sample is removed from the surface of the needle immediately Uncapped Wash Vial For best results the wash vial should contain solvent in which the sample components are soluble and the vial should not be capped If the wash vial is capped small amounts of sample remain on the surface of the septum which may be carried on the needle to the next sample Agilent 1260 Infinity Standard Autosampler User Manual Optimizing Performance 5 Injector Program with Needle Wash The injector
63. h ad ditional G1369C LAN Card The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN CAN connectors as interface to other modules LAN connector as interface to the control software RS 232C as interface to a computer REMOTE connector as interface to other Agilent products e Analog output connector s for signal output Agilent 1260 Infinity Standard Autosampler User Manual 161 11 Hardware Information 162 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosamp
64. he theta or X torque is not in the range proceed with step 6 on page 72 if you think you can adjust the torque otherwise proceed with step 7 on page 72 5 Exchange the gripper arm assembly part number G1313 60010 6 Adjust the belts tension If the measured torque value is too low the belt needs to be tightened If the measured torque value is too high the belt needs to be loosened For this slide the motor X or theta on the holder bracket in the appropriate direction and test the tension with the ALS Torque Verification in LabAdvisor Repeat this steps until the values are in the appropriate torque range 7 If these measures don t resolve the problem it is necessary to exchange the transport assembly or the main board To do this please contact your Agilent service representative 72 Agilent 1260 Infinity Standard Autosampler User Manual Troubleshooting and Diagnostics 6 Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the vial With Error messages motor overtemp 0 or 2 movement failed 0 or 2 WARNING Personal damage damage to the module gt Some of these procedures require a trained service engineer Persons who are not qualified MUST NOT perform these procedures When a motor over temperature message has occurred the sampler must be turned OFF for about 10 minutes to allow the motor to cool down 1 Check the cleanliness of the transport rods
65. he manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used Agilent 1260 Infinity Standard Autosampler User Manual 177 12 Appendix Safet
66. he needle fitting with the hole in the safety cover 4 Insert the new needle 1 Align the needle in the seat then tighten the screw firmly 2 112 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance 8 5 Reconnect the sample loop fitting to the needle fitting a av A Ku v 6 Use Needle Up to lift the needle to a position approximately 2 mm above the seat 2mm 7 Ensure the needle is aligned with the seat Next Steps 8 On completion of this procedure Install the front cover 9 Select End in the maintenance function Change Needle see Change Needle on page 60 Agilent 1260 Infinity Standard Autosampler User Manual 113 8 Maintenance Exchanging the Needle Seat Assembly When When the seat capillary is blocked Tools required Description When the seat is visibly damaged Wrench 1 4 inch supplied in HPLC Tool Kit Screwdriver flat head Parts required p n 1 G1329 87017 OR 1 G1329 87012 Preparations e Remove the front cover Description Needle seat assy 0 17 mm i d 2 3 uL Needle seat assy 0 12 mm i d 1 2 uL Select Start in the maintenance function Change Needle see Change Needle on page 60 Use the Needle Up command in the Change Needle function to lift the needle an addition 1 cm 1 Disconnect the seat capillary fitting from the injection valve port 5 2 Usea small fl
67. he slit Te XS SOOO OOOO erer SIOONI OIO OOOO S OOOO OOO T ceee COS e C 124 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance 8 Exchanging the Gripper Arm 3 Rotate the gripper arm slowly from left to right and apply 4 Hold the paper clip in place press the gripper release a gentle pressure to the paper clip The clip will engage button and rotate the gripper arm to the right on an internal catch and the rotation of the arm will be 6 Replace the gripper arm by holding the paper clip in place pushing the gripper arm into the holder and rotating the gripper arm to the left D Next Steps 7 On completion of this procedure Install the front cover 8 Turn the power to the autosampler ON Agilent 1260 Infinity Standard Autosampler User Manual 125 8 Maintenance Exchanging the Interface Board When At installation or when defective Tools required Description Screwdriver flat head Parts required Description 1 Interface board CAUTION Electronic boards are sensitive to electrostatic discharge ESD and should be handled with care so as not to damage them Touching electronic boards and components can cause electrostatic discharge ESD can damage electronic boards and components gt Be sure to hold the board by the edges and do not touch the electrical components Always use an ESD protection for example an ESD wrist strap
68. ial gripping X motor Theta motor Gripper motor Gripper Theta axis Z motor Flex board Figure 7 Transport Assembly The transport assembly uses four stepper motors driven in closed loop mode for accurate positioning of the gripper assembly The rotational movement of the motors is converted to linear motion X and Z axes by toothed belts connected to the drive spindles The rotation theta axes of the gripper assembly is transferred from the motor by a toothed belt and series of gears The opening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to the planetary gearing inside the gripper assembly 18 Agilent 1260 Infinity Standard Autosampler User Manual Introduction to the Autosampler ALS 1 The stepper motor positions are determined by the optical encoders mounted onto the stepper motor housing The encoders monitor the position of the motors continually and correct for position errors automatically e g if the gripper is accidentally moved out of position when loading vials into the vial tray The initialization positions of the moving components are sensed by reflection sensors mounted on the flex board These positions are used by the processor to calculate the actual motor position An additional six reflection sensors for tray recognition are mounted on the flex board at the front of the assembly Agilent 1260 Infinity Standard Autosampler User Manual 19 1
69. ic code 0 9 A Z where each combination unambiguously denotes a module there can be more than one code for the same module Serial number Aaien Technohpes Prosser No onma oogooggogug Figure 23 Autosampler Electrical Connections Agilent 1260 Infinity Standard Autosampler User Manual 171 11 Hardware Information Early Maintenance Feedback EMF 172 Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Ther
70. ice foam removed representative Agilent 1260 Infinity Standard Autosampler User Manual 87 7 Error Information Autosampler Error Messages 88 Front door open Error ID 4350 A sensor on the flex board measures the contact to the magnet in the front door If there is no contact and you try to start operation the error message is generated Probable cause Suggested actions 1 The door is not in place bent or the magnet Please contact your Agilent service is misplaced representative 2 The sensor on the flex board is defective Please contact your Agilent service representative Agilent 1260 Infinity Standard Autosampler User Manual Error Information 7 Arm Movement Failed Error ID 4002 The transport assembly was unable to complete a movement in one of the axes The processor defines a certain time window for the successful completion of a movement in any particular axis The movement and position of the transport assembly is monitored by the encoders on the stepper motors If the processor does not receive the correct position information from the encoders within the time window the error message is generated See figure Figure 7 on page 18 for axes identification Arm Movement 0 Failed X axis Arm Movement 1 Failed Z axis Arm Movement 2 Failed Theta gripper rotation Arm Movement 3 Failed Gripper gripper fingers open close Probable cause Suggested actions 1 Mechanical obstruction Ensur
71. ignment with the ChemStation enter following command in the command line Print sendmodule lals tray alig 0 00 0 00 If these measures don t resolve the problem it is necessary to exchange the transport assembly or the main board To do this please contact your Agilent service representative Agilent 1260 Infinity Standard Autosampler User Manual Troubleshooting and Diagnostics 6 Poor alignment With Error messages e motor overtemp 0 or 2 or 3 movement failed 0 or 2 or 3 WARNING Personal damage damage to the module gt Some of these procedures require a trained service engineer Persons who are not qualified MUST NOT perform these procedures When a motor over temperature message has occurred the sampler must be turned OFF for about 10 minutes to allow the motor to cool down 1 Reset the sampler alignment to default value Reset tray alignment and transport alignment is possible with the Control Module and the Chemstation To reset the transport alignment with the Chemstation enter following command in the command line Print sendmodule lals tray alig 0 00 0 00 2 Lubricate the X gear Friction can result in the belt slipping on the gear so that the position of the belt teeth towards the gear changes To avoid this apply some grease from the sample transport repair kit to the X motor gear Do not use other grease as the one in the kit and carefully follow the instruction from the techni
72. ilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module Agilent 1260 Infinity Standard Autosampler User Manual 127 8 Maintenance Replacing the Module s Firmware 128 Agilent 1260 Infinity Standard Autosampler User Manual Agilent 1260 Infinity Standard Autosampler User Manual 9 Parts and Materials for Maintenance Main Assemblies 130 Analytical Head Assembly 132 Vial Trays 134 Standard Autosampler Accessory Kit 136 Maintenance Kit 137 Injection Upgrade Kit 138 Multi Draw Kit 139 External Tray 140 This chapter provides information on parts for maintenance Apg Agilent Technologies 129 9 Parts and Materials for Maintenance Main Assemblies Main Assemblies Figure 18 Autosampler Main Assemblies 130 Agilent 1260 Infinity Standard Autosampler User Manual Parts and Materials for Maintenance 9 Item p n Description 1 01078 60003 Analytical head assembly 100 pL 2 G1313 60010 Gripper assembly 3 G1329 60011 Thermostattable tray for 100 x 2 mL vials 4 0101 1422 Injection valve G1351 68701 Interface board BCD with external contacts and BCD outputs 01090 87306 Capillary ST 0 17 mm x 380 nm S S Heat exchanger Agilent 1260 Infinity Standard Autosampler User Manual 131 9 Parts and Materials for Maintenance Analytica
73. ility for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards 176 Agilent 1260 Infinity Standard Autosampler User Manual Appendix 12 Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in t
74. in place Agilent 1260 Infinity Standard Autosampler User Manual Maintenance 8 Updating the Firmware The Modules are fitted with FLASH EPROMS These EPROMS enable you to update the instrument firmware from the LabAdvisor Instant Pilot G4208A LC Firmware Tool See also Replacing the Module s Firmware on page 127 Agilent 1260 Infinity Standard Autosampler User Manual 105 8 Maintenance Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 106 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance 8 Safety Flap Flex Board Safety Flap Flex Board It is strongly recommended that the exchange of the safety flap and flex board is done by Agilent trained service personnel Figure 17 Safety Flap Agilent 1260 Infinity Standard Autosampler User Manual 107 8 Maintenance Transport Assembly Parts The adjustment of the motors and the tension on the drive belts are important for correct operation of the transport assembly It is strongly recommended that exchange of drive belts and the gripper assembly is done by Agilent trained service personnel Ther
75. in the upper position Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Valve already in mainpass Valve not connected Defective injection valve Agilent 1260 Infinity Standard Autosampler User Manual 69 6 Troubleshooting and Diagnostics Troubleshooting Guide for the Sample Transport Assembly This troubleshooting guide is meant to help you diagnose and repair autosampler problems In general autosampler problems can be divided into three categories 1 Intermittent lock ups with or without vial in the gripper fingers with error messages Many times the sampler is being used very heavily motor overtemp 0 or 1 or 2 or 3 movement failed 0 or 1 or 2 or 3 missing vial Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the vial with error messages motor overtemp 0 or 2 movement failed 0 or 2 Poor alignment seen during vial pickup and vial replacement and or when the needle hits the gripper arm with error messages motor overtemp 0 or 2 or 3 movement failed 0 or 2 or 3 missing vial Motor 0 X 1 Z 2 Theta 3 Gripper 70 Agilent 1260 Infinity Standard Autosampler User Manual Troubleshooting and Diagnostics 6 Intermittent lock ups with or without vial in the gripper fingers With error messages e motor overtemp 0 or 1 or 2 or 3 movement failed 0 or 1 or 2 or 3 missing
76. ing Guide for the Sample Transport Assembly 70 Intermittent lock ups with or without vial in the gripper fingers 71 Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the vial 73 Poor alignment 75 Agilent Lab Advisor Software 77 Overview about the troubleshooting and diagnostic features eh Agilent Technologies 55 6 Troubleshooting and Diagnostics Overview of the Module s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Maintenance Functions The maintenance functions position the needle arm gripper assembly and metering device for easy access when doing maintenance see Maintenance Functions on page 59 Tray Alignment Tray alignment is required after repair of internal components or after a firmware update The procedure aligns the gripper arm correctly to ensure the positioning of the gripper arm is correct for all via
77. jection sequence and during an analysis the injection valve is in the mainpass position Figure 2 on page 13 In this position the mobile phase flows through the autosamplers metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry over Figure 2 Mainpass Position When the sample sequence begins the valve unit switches to the bypass position Figure 3 on page 13 Solvent from the pump enters the valve unit at port 1 and flows directly to the column through port 6 Figure 3 Bypass Position Agilent 1260 Infinity Standard Autosampler User Manual 13 1 Introduction to the Autosampler ALS Introduction to the Autosampler ALS Next the needle is raised and the vial is positioned below the needle The needle moves down into the vial and the metering unit draws the sample into the sample loop Figure 4 on page 14 t Figure 4 Drawing the Sample When the metering unit has drawn the required volume of sample into the sample loop the needle is raised and the vial is replaced in the sample tray The needle is lowered into the needle seat and the injection valve switches back to the mainpass position flushing the sample onto the column Figure 5 on page 14 Figure 5 Mainpass Position Sample Injection 14 Agilent 1260 Infinity Standard Autosampler User Manual Introduction to the Autosampler ALS 1 Introduction to the Autosampler
78. k is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface Agilent 1260 Infinity Standard Autosampler User Manual 81 7 Error Information General Error Messages Timeout Error ID 0062 The timeout threshold was exceeded Probable cause 1 The analysis was completed successfully and the timeout function switched off the module as requested 2 Anot ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Shutdown Error ID 0063 Suggested actions Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required An external instrument has generated a shutdown signal on the remote line The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrumen
79. l Head Assembly Analytical Head Assembly 100 pL I BE not shown Figure 19 Analytical Head Assembly Item p n Description 01078 60003 Analytical head assembly 100 pL 1 5063 6586 Sapphire piston 2 0515 0850 Screw M4 40 mm long 3 01078 23202 Adapter 4 5001 3739 Support Seal assembly 5 5063 6589 Metering seal pack of 2 for 100 uL analytical head 01078 27710 Head body 7 0515 2118 Screw M5 60 mm long 132 Agilent 1260 Infinity Standard Autosampler User Manual Parts and Materials for Maintenance 9 Analytical Head Assembly 900 pL Item p n G2260 60007 5062 8587 0515 0850 01078 23202 5001 3764 0905 1294 G2260 27700 0515 2118 Description Analytical head assembly 900 uL maximum pressure 400 bar only for G1329B Metering piston for 900 uL analytical head Screws Adapter Includes spring Support seal assembly 900 pL Metering seal 900 uL Head body 900 pL lt 400 bar Screw M5 60 mm long Upgrading the G1329A Standard Autosampler to 900 uL injection volume limits the maximum instrument pressure to 200 bar Therefore you must order the P N G1313 60007 for the G1329A instead of P N G2260 60007 This assembly includes the head body P N G1313 27700 for 200 bar Agilent 1260 Infinity Standard Autosampler User Manual 133 Parts and Materials for Maintenance Vial Trays Vial Trays Agilent 1260 Infinity Standard Autosampler User Manual 134 Item p n 1 G1329 43200 2
80. lace the piston guide on top of the seal 8 Reassemble the metering head assembly Carefully insert the piston into the base The closed side of the metering head must be on the same side as the lower one of the two capillary drillings 122 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance lt gt A J CCCa Aw 11 Reinstall the capillaries 9 Install the fixing bolts Tighten the bolts securely Ensure the large hole in the metering head is facing downwards Next Steps 12 On completion of this procedure Install the front cover 13 Select End in the maintenance function Change piston see Change Piston on page 62 10 Install the metering head assembly in the autosampler Agilent 1260 Infinity Standard Autosampler User Manual 123 8 Maintenance Exchanging the Gripper Arm Exchanging the Gripper Arm When Defective gripper arm Tools required Description Straightened paper clip Parts required p n Description 1 G1313 60010 Gripper assembly Preparations s Select Start in the maintenance function Change Gripper see Change Gripper on page 65 Turn off the power to the autosampler Remove the front cover 1 Identify the slit below the gripper motor and the gripper arm release button button 2 Rotate the arm approximately 2 5cm 1 inch to the left and insert the straightened paper clip into t
81. ler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as Agilent 1260 Infinity Standard Autosampler User Manual Hardware Information 11 Table 13 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 n 2 RX TX 3 X 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 V 7 RTS CTS 8 M 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 21 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board Agilent 1260 Infinity Standard Autosampler User Manual 163 11 Hardware Information 164 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Tech
82. lowing switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 20 Boot Resident Settings without on board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 0 0 1 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 21 Forced Cold Start Settings without on board LAN Mode Select sw1 sw2 SW3 sw4 SW5 SW6 SW7 SW8 TEST BOOT 1 1 0 0 0 0 0 1 Agilent 1260 Infinity Standard Autosampler User Manual 169 11 Hardware Information Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems The interface b
83. ls see Tray Alignment on page 65 Step Commands The step functions provide the possibility to execute each step of the sampling sequence individually The step functions are used primarily for troubleshooting and for verification of correct autosampler operation after repair see ALS Step Commands on page 66 56 Agilent 1260 Infinity Standard Autosampler User Manual Troubleshooting and Diagnostics 6 Status Indicators Status Indicators Two status indicators are located on the front of the autosampler The lower left indicates the power supply status the upper right indicates the autosampler status Status indicator ee eee Power supply indicator Figure 16 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON Agilent 1260 Infinity Standard Autosampler User Manual 57 6 Troubleshooting and Diagnostics Module Status Indicator The module status indicator indicates one of six possible module conditions 58 When the status indicator is OFF and power switch light is on the module is ina prerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition
84. ng In case the needle seat has got contaminated and carry over is significantly higher than expected the following procedure can be used to clean the needle seat Go to MORE INJECTOR and set needle to home position e Pipette an appropriate solvent on to the needle seat The solvent should be able to dissolve the contamination If this is not known use 2 or 3 solvents of different polarity Use several milliliters to clean the seat e Clean the needle seat with a tissue and remove all liquid from it RESET the injector 48 Agilent 1260 Infinity Standard Autosampler User Manual Optimizing Performance 5 Fast Injection Cycle and Low Delay Volume Short injection cycle times for high sample througput is one of the most important requirements in analytical laboratories In order to shorten cycle times you can e shorten the column length e use high flow rates apply a steep gradient Having optimized these parameters further reduction of cycle times can be obtained using the overlapped injection mode Overlapped Injection Mode In this process as soon as the sample has reached the column the injection valve is switched back to bypass and the next injection cycle starts but waits with switching to mainpass until the actual run is finished You gain the sample preparation time when using this process Switching the valve into the bypass position reduces the system delay volume the mobile phase is directed to the column withou
85. nologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as e standard TTL levels 0 V is logic true 5 0 V is false fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or Lwhen between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal Agilent 1260 Infinity Standard Autosampler User Manual Hardware Information 11 Table 14 Remote Signal Distribution Pin Sign
86. oard monitors the home position of the piston If the piston fails to move to the home position or if the sensor fails to recognize the piston position the error message is generated Probable cause Suggested actions 1 Dirty or defective sensor Please contact your Agilent service representative 2 Broken piston Exchange the metering piston and seal 3 Defective metering drive motor Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative Agilent 1260 Infinity Standard Autosampler User Manual 95 7 Error Information Motor Temperature Error ID 4027 4040 4261 4451 One of the motors of the transport assembly has drawn excessive current causing the motor to become too hot The processor has switched OFF the motor to prevent damage to the motor See figure Figure 7 on page 18 for motor identification Motor 0 temperature X axis motor Motor 1 temperature Z axis motor Motor 2 temperature Theta gripper rotation motor Motor 3 temperature Gripper motor motor for gripper fingers The processor monitors the current drawn by each motor and the time the motor is drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the time the motor draws current is too long the error message is generated Probable cause Suggested actions 1 Mechanical obs
87. oard slot is used for external contacts and BCD bottle number output or LAN connections The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 170 Agilent 1260 Infinity Standard Autosampler User Manual Serial Number Information Hardware Information 11 Electrical Connections The serial number information on the instrument labels provide the following information CCXZZ00000 cC ZZ 00000 Remote NOOOOO RS 232 CAN bus Format Country of manufacturing DE Germany JP Japan e CN China Alphabetic character A Z used by manufacturing Alpha numer
88. olvents e Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel e Halogenated solvents or mixtures which form radicals and or acids for example 2CHCl O gt 2COCl 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Agilent 1260 Infinity Standard Autosampler User Manual Appendix 12 Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides e Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel e Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Agilent 1260 Infinity Standard Autosampler User Manual 183 12 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive
89. on 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN Cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection 152 Agilent 1260 Infinity Standard Autosampler User Manual Auxiliary Cable f Identifying Cables 10 One end of this cable provides a modular plug to be connected to the Agilent vacuum degasser The other end is for general purpose Agilent Vacuum Degasser to general purposes p n G1322 81600 Color Pin Agilent Signal Name 1100 p White 1 Ground Brown Pressure signal Green 3 Yellow 4 Grey 5 DC 5VIN Pink 6 Vent Agilent 1260 Infinity Standard Autosampler User Manual 153 10 Identifying Cables RS 232 Cables 154 p n G1530 60600 RS232 61601 5181 1561 Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m Agilent 1260 Infinity Standard Autosampler User Manual Agilent 1260 Infinity Standard Autosampler User Manual 11 Hardware Information Firmware Description 156 Interfaces 159 Overview Interfaces 162 Set
90. onfigured mode All modules without on board LAN e default should be ALL DIPS DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 e DIP 1 DOWN and DIP 2 UP allows special RS 232 settings e for boot test modes DIPS 1 2 must be UP plus required mode For normal operation use the default best settings Switch settings provide configuration parameters for serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN Agilent 1260 Infinity Standard Autosampler User Manual Hardware Information 11 The following tables represent the configuration switch settings for the modules without on board LAN only Table 15 8 bit Configuration Switch without on board LAN Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 RSVD SYS RSVD RSVD FC The LAN settings are done on the LAN Interface Card G1369B C Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in ord
91. ormation 11 Firmware Updates Firmware updates can be done using your user interface PC and Firmware Update Tool with local files on the hard disk e Instant Pilot G4208A with files from a USB Flash Disk Agilent Lab Advisor software B 01 03 and above The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315AB for the G1315A B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 102 is revision 1 02 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Main FW update Resident System Main System Resident FW Update Figure 20 Firmware Update Mechanism Agilent 1260 Infinity Standard Autosampler User Manual 157 11 Hardware Information 158 Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the
92. otor Seal 400 bar Needle Seat Assy 0 17 mm Rotor seal Vespel 2 grooves max 400 bar Needle assembly Needle seat assy 0 17 mm i d 2 3 uL Description Metering seal pack of 2 for 100 uL analytical head Finger caps x3 Agilent 1260 Infinity Standard Autosampler User Manual 137 9 Parts and Materials for Maintenance Injection Upgrade Kit 138 Item p n G1363A G2260 60007 61313 87303 5022 2133 61313 87202 Description 900 uL Injection Upgrade Kit for G1329B Analytical head assembly 900 uL maximum pressure 400 bar for G1329B only Loop ext capillary 900 uL Union for 900 uL loop extension capillary Needle assembly 900 uL Agilent 1260 Infinity Standard Autosampler User Manual Multi Draw Kit p n G1313 87307 G1313 87308 0101 0301 5022 6515 Parts and Materials for Maintenance Description Seat capillary 500 uL 0 5 mm id Seat capillary 1500 uL 0 9 mm id Seat capillary 5000 pL Union ZDV Agilent 1260 Infinity Standard Autosampler User Manual 139 9 Parts and Materials for Maintenance External Tray p n Description G1313 60004 External tray G1313 27302 Disposal tube 140 Agilent 1260 Infinity Standard Autosampler User Manual This chapter provides information on cables used with the module Agilent 1260 Infinity Standard Autosampler User Manual 10 Identifying Cables Cable Overview 142 Analog Cables 144 Remote Cables 146 BCD Cables 149 External
93. parts identification please check the illustrated parts breakdown in Parts and Materials for Maintenance on page 129 Please report any missing or damaged parts to your local Agilent Technologies sales and service office Table5 Agilent 1260 Infintity Standard Autosampler Description Quantity Autosampler 1 Power Cable 1 User Documentation DVD 1 Agilent 1260 Infinity Standard Autosampler User Manual Installing the Autosampler 3 lt LontTigt Optimizing the Stack Configuration If your autosampler is part of a system you can ensure optimum performance by installing the autosampler in the stack in the position shown in Figure 8 on page 29 and Figure 9 on page 30 Figure 10 on page 31 and Figure 11 on page 31 show the configuration recommended for a thermostatted autosampler These configurations optimize the system flow path ensuring minimum delay volume HUN M DH Solvent cabinet Pump Instant Pilot Autosampler Column compartment Detector Figure 8 Recommended Stack Configuration for an Autosampler Front View Agilent 1260 Infinity Standard Autosampler User Manual 29 3 Installing the Autosampler Optimizing the Stack Configuration IN LI CAN Bus cable to Instant Pilot AC power CAN Bus cable LAN to LC Data Analog signal to recorder System Figure 9 Recommended Stack Configuration for an Autosampler Rear
94. positions The gripper arm places the sample vial below the injection needle The required volume of sample is drawn into the sample loop by the metering device Sample is applied to the column when the injection valve returns to the mainpass position at the end of the sampling sequence The sampling sequence occurs in the following order 1 The injection valve switches to the bypass position 2 The piston of the metering device moves to the initialization position 3 The gripper arm moves from the home position and selects the vial At the same time the needle lifts out of the seat The gripper arm places the vial below the needle The needle lowers into the vial The metering device draws the defined sample volume The needle lifts out of the vial onion A If the automated needle wash is selected see Using the Automated Needle Wash on page 46 the gripper arm replaces the sample vial positions the wash vial below the needle lowers the needle into the vial then lifts the needle out of the wash vial 9 The gripper arm checks if the safety flap is in position 10 The gripper arm replaces the vial and returns to the home position Simultaneously the needle lowers into the seat 11 The injection valve switches to the mainpass position Agilent 1260 Infinity Standard Autosampler User Manual Introduction to the Autosampler ALS 1 Introduction to the Autosampler ALS Injection Sequence Before the start of the in
95. r for the circulation of air Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 24 Agilent 1260 Infinity Standard Autosampler User Manual Site Requirements and Specifications 2 Physical Specifications Table3 Physical Specifications Type Specification Comments Weight 14 2 kg 32 Ibs Dimensions 200 x 345 x 435 mm height x width x depth 8 x 13 5 x 17 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 300 VA 200 W 683 BTU Maximum Ambient operating 0 55 C 32 131 F See warning Hot rear temperature panel on page 25 Ambient non operating 40 70 C 40 158 F temperature Humidity lt 95 r h at 40 C 104 F Non condensing Operating altitude Up to 2000 m 6562 ft Non operating altitude Up to 4600 m 15091 ft For storing the module Safety standards Installation category II Pollution degree2 For indoor use only IEC CSA UL Using the module at high environmental temperatures may cause the rear panel to become hot gt Do not use the module at environmental temperatures higher
96. r messages and provides information on probable causes and suggested actions how to recover from error conditions Agilent 1260 Infinity Standard Autosampler User Manual 3 In This Book 8 Maintenance This chapter describes the maintenance of the module 9 Parts and Materials for Maintenance This chapter provides information on parts for maintenance 10 Identifying Cables This chapter provides information on cables used with the module 11 Hardware Information This chapter describes the detector in more detail on hardware and electronics 12 Appendix This chapter provides addition information on safety legal and web 4 Agilent 1260 Infinity Standard Autosampler User Manual Contents Contents 1 Introduction to the Autosampler ALS 9 Introduction to the Autosampler ALS 10 2 Site Requirements and Specifications 21 Site Requirements 22 Physical Specifications 25 Performance Specifications Standard Autosampler 26 3 Installing the Autosampler 27 Unpacking the Autosampler 28 Optimizing the Stack Configuration 29 Installing the Autosampler 32 Flow Connections 35 Installing the Sample Tray 37 Transporting the Autosampler 38 4 Usingthe Autosampler 39 Sample Trays 40 Choice of Vials and Caps 42 5 Optimizing Performance 45 Optimization for Lowest Carry over 46 Fast Injection Cycle and Low Delay Volume 49 Precise Injection Volume 51 Choice of Rotor Seal 53 6 Troubleshooting and Diagnostics 55
97. raw speed 200 pL min ejection speed 200 pL min injection volume 5 pL Metering pump in high pressure flow path Agilent 1260 Infinity Standard Autosampler User Manual Agilent 1260 Infinity Standard Autosampler User Manual 3 Installing the Autosampler Unpacking the Autosampler 28 Optimizing the Stack Configuration 29 Installing the Autosampler 32 Flow Connections 35 Installing the Sample Tray 37 Transporting the Autosampler 38 This chapter provides information on unpacking checking on completeness stack considerations and installation of the module Ee Agilent Technologies 27 3 Installing the Autosampler Unpacking the Autosampler Damaged Packaging CAUTION If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Delivery Checklist 28 Ensure all parts and materials have been delivered with your module The delivery checklist is shown below For
98. rd while the sample volume is determined by counting the number of steps from the home position The backward movement of the piston driven by the spring draws sample from the vial Table1 Analytical Head Technical Data Standard 100 pL Extended Volume 900 pL Number of steps 15000 15000 Volume resolution 7 nL motor step 60 nL motor step Maximum stroke 100 uL 900 uL Pressure limit 600 bar 400 bar G1329B 200 bar G1329A Piston material Sapphire Sapphire Agilent 1260 Infinity Standard Autosampler User Manual Introduction to the Autosampler ALS 1 Injection Valve The two position 6 port injection valve is driven by a stepper motor Only five of the six ports are used port 3 is not used A lever slider mechanism transfers the movement of the stepper motor to the injection valve Two microswitches monitor the switching of the valve bypass and mainpass end positions No valve adjustments are required after replacing internal components Table 2 Injection Valve Technical Data Autosampler Motor type 4V 1 2 A stepper motor Seal material PEEK Stator material None Number of ports 6 Switching time lt 150 ms Agilent 1260 Infinity Standard Autosampler User Manual 17 1 Introduction to the Autosampler ALS Introduction to the Autosampler ALS Transport Assembly The transport unit comprises an X axis slide left right motion a Z axis arm up down motion and a gripper assembly rotation and v
99. rence points Because the tray is a rectangle a two point alignment is sufficient to correct all other vial positions within the tray On completion of the procedure the corrected gripper positions are stored in the instrument firmware Agilent 1260 Infinity Standard Autosampler User Manual 65 ALS Step Commands 66 Troubleshooting and Diagnostics Each movement of the sampling sequence can be done under manual control This is useful during troubleshooting where close observation of each of the sampling steps is required to confirm a specific failure mode or verify successful completion of a repair Each injector step command actually consists of a series of individual commands that move the autosampler components to predefined positions enabling the specific step to be done Table 6 Step Valve Bypass Plunger Home Needle Up Vial to Seat Needle into Sample Draw Needle Up Vial to Tray Injector step commands Action Switches injection valve to the bypass position Moves the plunger to the home position Lifts the needle arm to the upper position Moves the selected vial to the seat position Lowers the needle into the sample Metering device draws the defined injection volume Lifts the needle out of the vial Returns the selected vial to the tray position Comments Command also switches the valve to bypass if it is not already in that position Command also lifts the needle
100. riate maintenance position In the Data System the ALS maintenance positions can be selected from the Maintenance menu in the Diagnosis display User Interface The functions for the control software are Change Needle moves the safety flap away from the needle and positions the needle arm for easy access to the needle and needle seat Change Piston relieves the tension on the metering spring draws the piston to the outer position enabling easy disassembly of the metering head assembly Park Arm secures the gripper arm to the park position behind the sampling unit ready for transport or shipping of the autosampler Home moves the tray arm to its home position for better access and exchange of the trays Change Gripper The change gripper function moves the gripper to the front of the autosampler enabling easy access to the gripper release mechanism Agilent 1260 Infinity Standard Autosampler User Manual 59 6 Troubleshooting and Diagnostics Change Needle For needle exchange the needle arm moves down automatically when the front cover is removed Risk of personal injury due to moving needle gt Keep fingers away from the needle area during needle movement 60 The change needle seat function moves the safety flap out of position and positions the needle for easy exchange and alignment of the needle and needle seat User Interface The commands for the Data System are The autosampler front
101. rking with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere Agilent 1260 Infinity Standard Autosampler User Manual 103 8 Maintenance CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module 104 The electronics of the autosampler will not allow operation of the autosampler when the top cover and the top foam are removed A safety light switch on the main board will inhibit the operation of the fan immediately Voltages for the other electronic components will be turned off after 30 seconds The status lamp will light up red and an error will be logged into the logbook of the user interface Always operate the autosampler with the top covers
102. rom the needle area during autosampler operation gt Do not bend the safety flap away from its position or attempt to remove the safety cover gt Do not attempt to insert or remove a vial from the gripper when the gripper is positioned below the needle 32 Agilent 1260 Infinity Standard Autosampler User Manual Installing the Autosampler 3 CAUTION Defective on arrival problems lf there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Install the LAN interface board in the sampler if required Remove the adhesive tape which covers the front door Remove the front door and remove the transport protection foam PF wWRr Place the Autosampler on the bench or in the stack as recommended in Optimizing the Stack Configuration on page 29 Ensure the power switch at the front of the Autosampler is OFF Connect the power cable to the power connector at the rear of the sampler Connect the CAN cable to the other modules on oo ol If an Agilent ChemStation is the controller connect the LAN connector to the LAN interface 9 Connect the APG remote cable optional for all instruments other than Agilent 1200 Infinity S
103. ropylene septum clear PTFE red rubber 100 Pack Snap Cap red polypropylene septum clear PTFE red rubber 100 Pack Description Screw Cap blue polypropylene septum clear PTFE red rubber 100 Pack Screw Cap green polypropylene septum clear PTFE red rubber 100 Pack Screw Cap red polypropylene septum clear PTFE red rubber 100 Pack Screw Cap blue polypropylene septum clear PTFE silicone 100 Pack Screw Cap green polypropylene septum clear PTFE silicone 100 Pack Screw Cap red polypropylene septum clear PTFE silicone 100 Pack Agilent 1260 Infinity Standard Autosampler User Manual Agilent 1260 Infinity Standard Autosampler User Manual e Optimizing Performance J Optimization for Lowest Carry over 46 Using the Automated Needle Wash 46 Using an Injector Program 47 General Recommendation to Lowest Carry over 48 Fast Injection Cycle and Low Delay Volume 49 Overlapped Injection Mode 49 General Recommendations for Fast Injection Cycle Times 50 Precise Injection Volume 51 Draw and Eject Speed 51 Choice of Rotor Seal 53 This chapter provides information on how to optimize the module ee Agilent Technologies 45 5 Optimizing Performance Optimization for Lowest Carry over 46 Several parts of an injection system can contribute to carry over e needle outside e needle inside e needle seat e sample loop e seat capillary e injection valve The autosampler continuous flow through desi
104. s 10 minutes See Exchanging the Needle Assembly on page 111 See Exchanging the Needle Seat Assembly on page 114 See Exchanging the Rotor Seal on page 116 See Exchanging the Metering Seal and Piston on page 120 See Exchanging the Gripper Arm on page 124 110 Agilent 1260 Infinity Standard Autosampler User Manual Maintenance 8 Exchanging the Needle Assembly When When the needle is visibly damaged When the needle is blocked Tools required Description Wrench 1 4 inch supplied in HPLC Tool Kit Hexagonal key 2 5 mm supplied in HPLC Tool Kit Pair of pliers Parts required p n Description 1 G1313 87201 Needle assembly OR 1 G1313 87202 Needle assembly 900 uL Preparations Select Start in the maintenance function Change Needle see Change Needle on page 60 When the needle is positioned approx 15 mm above the needle seat remove the front cover _ WARNING fie To avoid personal injury keep fingers away from the needle area during autosampler operation gt Do not bend the safety flap away from its position or attempt to remove the safety cover gt Do not attempt to insert or remove a vial from the gripper when the gripper is positioned below the needle Agilent 1260 Infinity Standard Autosampler User Manual 111 8 Maintenance Exchanging the Needle Assembly 1 Select Needle Down until the needle screw is aligned 2 Remove the sample loop fitting from t
105. sis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected e Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system Agilent 1260 Infinity Standard Autosampler User Manual 83 7 84 Error Information Leak Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 Leaking rotor seal or needle seat Exchange the rotor seal or seat capillary 4 Defective metering seal e Exchange the metering seal Make sure the
106. t passing sample loop needle and needle seat capillary This can help to have faster cycle times especially if low flow rates have to be used like it is mandatory in narrow bore and micro bore HPLC Having the valve in bypass position can increase the carry over in the system The injection cycle times also depend on the injection volume In identically standard condition injecting 100 ul instead of 1 ul increase the injection time by approximately 8 sec In this case and if the viscosity of the sample allows it the draw and eject speed of the injection system has to be increased Agilent 1260 Infinity Standard Autosampler User Manual 49 5 50 Optimizing Performance For the last injection of the sequence with overlapped injections it has to be considered that for this run the injection valve is not switched as for the previous runs and consequently the injector delay volume is not bypassed This means the retention times are prolonged for the last run Especially at low flow rates this can lead to retention time changes which are too big for the actual calibration table To overcome this it is recommended to add an additional blank injection as last injection to the sequence General Recommendations for Fast Injection Cycle Times As described in this section the first step to provide short cycle times are optimizing the chromatographic conditions If this is done the autosampler parameter should be set to e Overlapped inje
107. t with a remote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition Check the vacuum degasser for an error condition Refer to the Service Manual for the degasser or the 1260 pump that has the degasser built in Agilent 1260 Infinity Standard Autosampler User Manual Error Information 7 Remote Timeout Error ID 0070 A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause Suggested actions 1 Not ready condition in one of the Ensure the instrument showing the not ready instruments connected to the remote line condition is installed correctly and is set up correctly for analysis 2 Defective remote cable Exchange the remote cable 3 Defective components in the instrument Check the instrument for defects refer to the showing the not ready condition instrument s documentation Lost CAN Partner Error ID 0071 During an analy
108. the analytical head assembly can be removed and reinstalled easily after maintenance User Interface The commands for the control software are Start Draws the piston away from the home position relieving the tension on the spring End Repositions the piston at the home position Using the Change Seal Function 1 Select Start to move the piston to the maintenance position 2 Exchange the metering seal see Exchanging the Gripper Arm on page 124 3 Select End to move the piston back to the home position 62 Agilent 1260 Infinity Standard Autosampler User Manual Troubleshooting and Diagnostics 6 Park Arm User Interface In the control software the Park Arm command is part of the ALS maintenance positions that can be selected from the Maintenance menu in the Diagnosis display The commands for the control software are Park Arm moves the gripper arm to the park position Home moves the gripper arm out of the park position to the home position Agilent 1260 Infinity Standard Autosampler User Manual 63 6 Troubleshooting and Diagnostics When CAUTION To prepare autosampler for transportation The park arm function moves the gripper and transport slider to the home position behind the sampling unit and lowers the gripper arm into the park position where the transport assembly is secured against a mechanical stop The autosampler can be switched OFF after parking the arm Before transporting
109. ting the 8 bit Configuration Switch 166 Communication Settings for RS 232C 167 Special Settings 169 Electrical Connections 170 Serial Number Information 171 Electrical Connections 170 Early Maintenance Feedback EMF 172 Instrument Layout 173 This chapter describes the detector in more detail on hardware and electronics ott Agilent Technologies 155 11 Hardware Information Firmware Description The firmware of the instrument consists of two independent sections e anon instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote error handling diagnostic functions e or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 156 Agilent 1260 Infinity Standard Autosampler User Manual Hardware Inf
110. to the needle seat position If the needle fails to reach the end point or if the sensor fails to recognize the needle arm movement the error message is generated Probable cause 1 Needle installed incorrectly or wrong needle type too long 2 Defective or dirty position sensor 3 Defective motor 4 Sticking spindle assembly 5 Defective main board Suggested actions Ensure the correct needle type is used and installed correctly Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1260 Infinity Standard Autosampler User Manual Error Information 7 Missing Vial Error ID 4019 4034 4541 4706 No vial was found in the position defined in the method or sequence When the gripper arm picks a vial out of the sample tray the processor monitors the gripper motor encoder If a vial is present the closing of the gripper fingers is limited by the vial However if no vial is present the gripper fingers close too far This is sensed by the processor encoder position causing the error message to be generated Probable cause Suggested actions 1 Novialin the position defined inthe method Install the sample vial in the correct position or or sequence edit the method or sequence accordingly 2 Incorrect gripper alignment Align gripper 3 Defecti
111. ton Home Defective or dirty sensor on the sampling unit flex board Defective metering drive motor Needle Up Needle already in the upper position Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Vial to Seat No vial in selected position Vial already in seat position Defective transport assembly motors Sticking transport assembly Defective gripper assembly Gripper not aligned see Tray Alignment on page 65 Draw Sum of all draw volumes exceeds 100uI Defective metering drive motor Needle Up Needle already in the upper position Needle already in the upper position Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Agilent 1260 Infinity Standard Autosampler User Manual Troubleshooting and Diagnostics 6 Table7 Step Failures Step Function Probable Failure Modes Vial to Tray Defective transport assembly motors Sticking transport assembly Defective gripper assembly Gripper not aligned see Tray Alignment on page 65 Needle Down Needle already in the lower position Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Mainpass Valve already in mainpass Valve not connected Defective injection valve Needle Up Mainpass Blockage in the sample loop or needle no solvent flow Needle already
112. truction Ensure unobstructed movement of the transport assembly 2 High friction in the transport assembly Please contact your Agilent service representative 3 Motor belt tension too high Switch OFF the autosampler at the power switch Wait at least 10 min before switching on again 4 Defective motor Please contact your Agilent service representative 5 Defective transport assembly flex board Please contact your Agilent service representative 96 Agilent 1260 Infinity Standard Autosampler User Manual Error Information 7 Initialization with Vial Error ID 4028 The autosampler attempted to initialize with a vial still in the gripper During initialization the autosampler checks correct operation of the gripper by closing and opening the gripper fingers while monitoring the motor encoder If a vial is still in the gripper when initialization is started the gripper fingers cannot close causing the error message to be generated Probable cause Suggested actions 1 Vial still in gripper Remove the vial using the Release Vial function in the user interface Reinitialize the autosampler Safety Flap Missing Error ID 4032 The safety flap was not detected Before the needle moves down into the needle seat to inject sample the safety flap locks into position Next the gripper checks the safety flap by trying to move the safety flap away from the needle If the gripper is able to move beyond the safety flap position sa
113. ty Standard Autosampler User Manual 3 37 3 Installing the Autosampler Transporting the Autosampler 38 When moving the autosampler around the laboratory no special precautions are needed However if the autosampler needs to be shipped to another location via carrier ensure The transport assembly is parked see Park Arm on page 63 e The vial tray is secured If the autosampler is to be shipped to another location the transport assembly must be moved to the park position to prevent mechanical damage should the shipping container be subjected to excessive shock Also ensure the vial tray is secured in place with suitable packaging otherwise the tray may become loose and damage internal components Agilent 1260 Infinity Standard Autosampler User Manual 7 Agilent 1260 Infinity Standard Autosampler User Manual KP amp e o o ee 4 aa e 07o Using the Autosampler e e e Sample Trays 40 Choice of Vials and Caps 42 This chapter provides information on how to set up the module for an analysis and explains the basic settings gy Agilent Technologies 39 4 Using the Autosampler Sample Trays 40 Supported trays for the module p n Description G1313 44510 Tray for 100 x 2 mL vials G1313 44513 Halftray for 15 x 6 mL vials G1313 44512 Halftray for 40 x 2 mL vials G1329 60011 Thermostattable tray for 100 x 2 mL vials Half Tray Combinations Half trays can be installe
114. uL min This speed is suitable for the majority of applications however when using viscous samples set the draw speed to lower speed for optimum results A DRAW statement in an injector program also uses the draw speed setting which is configured for the autosampler Agilent 1260 Infinity Standard Autosampler User Manual 51 5 Optimizing Performance Eject Speed The default eject speed setting is 200 uL min When using large injection volumes setting the eject speed to a higher value speeds up the injection cycle by shortening the time the metering unit requires to eject solvent at the beginning of the injection cycle when the piston returns to the home position An EJECT statement in an injector program also uses the eject speed setting which is configured for the autosampler A faster eject speed shortens the time required to run the injector program When using viscous samples a high eject speed should be avoided 52 Agilent 1260 Infinity Standard Autosampler User Manual Optimizing Performance 5 Choice of Rotor Seal Vespel Seal for standard valves only The standard seal has sealing material made of Vespel Vespel is suitable for applications using mobile phases within the pH range of 2 3 to 9 5 which is suitable for the majority of applications However for applications using mobile phases with pH below 2 3 or above 9 5 the Vespel seal may degrade faster leading to reduced seal lifetime Tefzel Seal for stan
115. ve gripper assembly defective Exchange the gripper assembly gripper fingers or belt 4 Defective transport assembly flex board Please contact your Agilent service representative Agilent 1260 Infinity Standard Autosampler User Manual 93 7 94 Error Information Initialization Failed Error ID 4020 The autosampler failed to complete initialization correctly The autosampler initialization procedure moves the needle arm and transport assembly to their home positions in a predefined sequence During initialization the processor monitors the position sensors and motor encoders to check for correct movement If one or more of the movements is not successful or is not detected the error message is generated Probable cause 1 Mechanical obstruction Defective sampling unit flex board Defective transport assembly flex board Defective sampling unit motor Defective main board Suggested actions Ensure unobstructed movement of the transport assembly Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Agilent 1260 Infinity Standard Autosampler User Manual Error Information 7 Metering Home Failed Error ID 4054 4704 The metering piston has failed to move back to the home position The home position sensor on the sampling unit flex b
116. vial WARNING Personal damage damage to the module gt Some of these procedures require a trained service engineer Persons who are not qualified MUST NOT perform these procedures When a motor over temperature message has occurred the sampler must be turned OFF for about 10 minutes to allow the motor to cool down 1 Check the vials and the caps For reliable operation vials used with the autosampler must not have tapered shoulders or caps that are wider than the body of the vial For more details see the service note G1313 017 2 Very heavy usage use a macro A pre sequence macro inj_rset mac will automatically reset the sampler at the start of a sequence ChemStation 3 Reset the sampler alignment to default value Reset tray alignment and transport alignment is possible with the Control Module and the ChemStation To reset the transport alignment with the ChemStation enter following command in the command line Print sendmodule lals tray alig 0 00 0 00 Agilent 1260 Infinity Standard Autosampler User Manual 71 6 Troubleshooting and Diagnostics 4 Check the tension of the belts For this use the ALS Torque Verification in LabAdvisor and measure the torque for each axis Table 8 Typical ranges Theta both 30 50 X axis both 50 90 Z axis both 90 130 Gripper open 30 65 Gripper closed maximum 30 If the Gripper open closed torque is not in the range proceed with step 5 on page 72 If t
117. y Symbols Table 22 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available A and the user should not touch it when heated up WARNING aia alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 178 Agilent 1260 Infinity Standard Autosampler User Manual Appendix 12 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed

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