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Typhoon™ FLA 9000

1. Maintenance of the SHG laser If you use the SHG laser in the Typhoon FLA 9000 it will require periodical calibration When the Typhoon FLA 9000 is switched on it automatically executes calibration You do not need to do a manual calibration if the Typhoon FLA 9000 is used at least once a month CAUTION AN Activate the Typhoon FLA 9000 at least once every 30 days to execute calibra tion This calibration ensure that the SHG laser operates properly Failure to calibrate the Typhoon FLA 9000 every 30 days reduces the lifespan of the SHG laser Operation procedures 1 Turn on the Typhoon FLA 9000 and the computer 2 Wait until warm up and self diagnosis is completed and the scanner is ready Only the power lamp on the upper left panel is lit The automatic calibration has now been performed 3 Turn off the Typhoon FLA 9000 and the computer Cleaning the stage CAUTION Wear gloves to prevent direct contact with chemical substances Step Action 1 Remove the stage from the main body of the instrument 2 Wipe the stage with a sponge moistened with a fluorescence free neutral detergent 3 Thoroughly rinse the stage with water and dry with a lint free cloth 4 Place the stage in the main body of the instrument Typhoon FLA 9000 User Manual 28 9607 65 AB 91 12 Maintenance Storing the Fluor or Multi stage Store the Fluor or Multi stage in the original packaging when they are not in use DO NOT
2. B Specifications Power supply Input voltage 100 to 240V AC single phase Allowable variations in volt 10 age Frequency 50 to 60 Hz Rated current 1 5 to 3 0 A Environmental conditions Parameter Data Operating conditions Temperature 15 C to 30 C Humidity 20 to 75 RH no dew condensation Non operating conditions Temperature 10 C to 40 C Humidity 20 to 70 RH no dew condensation Transportation amp storage temperature Temperature 25 C to 70 C Humidity 10 to 80 no dew condensation Heat radiation 151 W h reader block FLA Image eraser Lighting It is recommended to lower the lighting level to about 20 lux when moving a sample from the cassette into Typhoon FLA 9000 after exposure Where to use Indoor use only Maximum altitude for use 2 000 m above sea level Overvoltage category Transient overvoltage category II Applicable rated pollution degree Pollution Degree 2 Noise levels Parameter Noise 70 dB A or lower 102 Typhoon FLA 9000 User Manual 28 9607 65 AB B Specifications Parameter Degrees of protection provid IP20 ed by enclosure Typhoon FLA 9000 User Manual 28 9607 65 AB 103 For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences
3. Parts and functions Instrument body aeee e EE Table of Contents Table of Contents 10 11 12 13 OF ENG IN acs caespciseecs scstesccececetecescsesdccese E osvseeercaeossvesseeneoeeseets 62 91 Operation OVerViEW se en erent tre ak ee ete E EE eee ee meme ey eee Crem meet eer ener 62 9 2 Preparations before starting Typhoon FLA 9000 wssccsccasscsssssscssccinscssosscosscasssosssivsoossavecsnneanovassivsonnccancavsedoessveneeedtens 62 9 3 Starting Typhoon FLA 9000 and Typhoon FLA 9000 Control Software sseessssssssseesssccsssessscccssseessccsessees 63 g4 Sel cting the redding MOTE a eccicoscassceesconsaseznstoqusstszecsonsbazntecnceonasanecasanasehossana untedesoassstuacosasissdetgasnzssaceoagassastceconaszeceneosiced 66 OS Setting the reading Conditions s sssssss1s111ss11ss1sstsssorntsstrnotssorntsssnesntssntsnnorsnessnrnntssornorsnnrsntssorsnnornnesntrn ontenan neeaae 67 9 6 Placing the object to be scanned on the stage x ciicjcisiesaahisseaienioseieisicdanentianescetinnimshaesgsdneses 67 9 7 Placing the stage in Typhoon FLA 9000 ssssss1ss1sssssstssstsssorsntssnrssotssorntssontsnnssntssnornossnrsotssornorrsnoonrnnessnorsnenns 72 98 Prescon the sample optional seyrini Ea REREN 74 99 Scanning thespian E RER I TASAR E A R 74 9 10 Adj sting the display CNT ME S rarisnerensisisiniensianisnnannonanannsniun nii inin 75 9 11 Saving the image data using a different file NAME eeccsssssssssssssecesesssessssssessececcsss
4. 2 Fluorescence button click to read a fluorescent sample 3 Chemiluminescence button click to read a chemiluminescent sample 4 Digitization button click to perform digitization 5 Method button click to register or erase a combination of laser and filter 6 Filter Module click to change or register a filter 7 Preferences click to select file format and correction mode 8 Filter displays the loaded filters 9 Laser displays the status of the loaded laser units 10 PMT displays the status of the loaded photo multiplier tube Overview of the reader settings window weet Gaerne et Gam E emie 4 Pai r 28 wm O pm EJ ym C20 pm ae To vaw rg us to 5 wee J Bi x Ra Le 6 7 File Size about 6 01 MB oe 10 Reading Tine Banor c O 12 11 Top File Format Gel B Prescan O Start scan J 14 13 Part Description 1 Image folder specify where to save the file after the reading 2 File Name enter the name of a file to save image data 3 Comment enter an optional comment The comment is embedded in the file where the image is saved and can be viewed with the analyzing software 4 Method set the method to use in the scan Up to 4 scans can be performed in a row all with individual methods Typhoon FLA 9000 User Manual 28 9607 65 AB 11 1 Introduction 1 4 Typhoon FLA 9000 Control Software Part Description 5 PMT set the voltage of the photo mu
5. 680 683 710 BPFR700 IRDye 700 689 700 BPFR700 Krypton Infrared 690 718 BPFR700 785 nm laser Reagent name Excitation wavelength Emission light wavelength Filter Alexa Fluor 750 746 775 BPFR800 Alexa Fluor 790 784 814 BPFR800 DY 781 783 800 BPFR800 IRDye 800 778 806 BPFR800 100 Typhoon FLA 9000 User Manual 28 9607 65 AB Appendix B Specifications Scanning specifications B Specifications Parameter Data Scanned image size 40 x 46 cm Pixel size 10 25 50 100 or 200 um and a 1000 um prescan Gradation bit depth 16 bit Dynamic range Five orders of magnitude Image capacity e 3510 MB 10 um e 561 52 MB 25 um e 140 35 MB 50 um e 35 09 MB 100 um e 8 77 MB 200 pm Detection sensitivity e Storage phosphor screen C Detectable to 0 9 dom mm e Fluorescent DNA SYBR Green 7 pg band e Enzyme multiplied fluorescence sensitivity pBR328 AttoPhos 100 fg spot Outside dimensions amp weight Dimensions amp weight Unit Dimension mm Weight kg wxdxh Reading block 900 x 800 x 400 97 projections not includ ed Storage phosphor screen cassette 460 x 430 x 20 Approx 2 5 Phosphor stage 502 x 538 x45 Approx 2 5 Multi stage 502 x 538 x45 Approx 2 8 Fluor stage 502 x 538 x45 Approx 2 3 FLA Image Eraser 603 x 512 x 164 14 5 Typhoon FLA 9000 User Manual 28 9607 65 AB 101
6. 9000 Step Action 1 Open the lid of the Typhoon FLA 9000 2 Place the stage on the Typhoon FLA 9000 with the printed side of the frame facing up 72 Typhoon FLA 9000 User Manual 28 9607 65 AB 9 Operation 9 7 Placing the stage in Typhoon FLA 9000 Step Action 3 Press the stage all the way in 4 Close the lid Placing the LF glass plate stage in the Typhoon FLA 9000 Step Action 1 Open the lid of the Typhoon FLA 9000 2 Place the LF glass plate stage in the scanner 3 Push the LF glass plate stage in until it stops 4 Close the lid Typhoon FLA 9000 User Manual 28 9607 65 AB 73 9 Operation 9 7 Placing the stage in Typhoon FLA 9000 Placing the phosphor stage in Typhoon FLA 9000 Step Action 1 2 Open the lid of the Typhoon FLA 9000 Position the phosphor stage with the storage phosphor screen face down Letetutatedete es aetototatetatms stain Push the phosphor stage inwards until it stops Close the lid of the Typhoon FLA 9000 9 8 Prescan the sample optional Note Step The prescan feature is not available in the phosphorimaging mode Action 1 3 Click the Prescan button The sample is scanned at low resolution and the result is dis played in the program window of Typhoon FLA 9000 Control Software Adjust the display parameters if desired Follow the instructions in Section 10 2 Display parameters explained on page 83 Click Retur
7. AN tC Range Scope Intensity graph Sieen Gry 3 Color ee Magnification 101 10 JAS pam magnification ee e o eun Magnification Select a ratio to zoom in and out of the display area If the magnification is high scroll bars appear on the right and bottom edges of the display area Use these scrollbars to view different parts of the display area There are several ways to zoom in and out of the display area see the table below Zooming in Zooming out Click the magnifying glass symbol and left click Click the magnifying glass symbol and right the display area click the display area or or Click the magnifying glass symbol and right Click the magnifying glass symbol and left click click the display area the display area or or select a magnification ratio further down the select a magnification ratio further up the Mag Magnification drop down list nification drop down list Typhoon FLA 9000 User Manual 28 9607 65 AB 83 10 Reading conditions display parameters and other settings 10 2 Display parameters explained Intensity graph The intensity graph displays the intensity levels of the image in histogram format The Low and High values below the graph correspond to the range of light intensities displayed These values are displayed in the graph as vertical red lines To display a larger or smaller interval of light intensities drag the lines using the mouse to adjust the Low and High values Curve
8. Alexa Fluor 680 ch 2 BD BPFR700 ch 2 Alexa Fluor 700 ch 2 BD BPFR700 ch 2 Alexa Fluor 750 ch 2 BD BPFR800 ch 2 Alexa Fluor 790 ch 2 BD BPFR800 ch 2 AttoPhos E LPB ch 1 CBB W LPG ch 1 Chemiluminescencel NM Through ch 1 2 Select the desired method to edit 3 Click the Edit button 4 Enter the desired changes 5 Click the OK button 6 Finalize the changes by clicking the OK button in the Method Settings dialog box Note Default methods cannot be edited Names of default methods are surrounded by square brackets 7 3 Deleting a method Note Default methods cannot be deleted Names of default methods are surrounded by square brackets Typhoon FLA 9000 User Manual 28 9607 65 AB 47 7 Registering editing and deleting methods 7 3 Deleting a method Step Action 1 Click the Method button in the main window The Method Settings dialog box appears 3 Method settings Method Settings Method List Name Alexa Fluor 488 LPB ch 1 B LPs ch 1 Alexa Fluor 555 E LPG ch 1 Alexa Fluor 647 LPR ch 1 Alexa Fluor 680 ch 2 P BPFR700 ch 2 Alexa Fluor 700 ch 2 FJ BPFR700 ch 2 Alexa Fluor 750 ch 2 BPFR800 ch 2 Alexa Fluor 790 ch 2 DB BPFR800 ch 2 E LPB ch 1 B LPG ch 1 N Through ch 1 2 Select the desired method to delete 5 Click the Delete button 4 Click the Yes button A confirmation dialog appears In
9. MD 2006 95 EC Low Voltage Directive LVD 2004 108 EC ElectroMagnetic Compatibility EMC Directive Typhoon FLA 9000 User Manual 28 9607 65 AB 1 Introduction 1 2 Regulatory information CE Marking The CE marking and the corresponding Declaration of Conformity is valid for the instrument when it iS used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in the user documentation and used in the same state as it was delivered from GE Healthcare except for alterations described in the user documentation Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numerique de la class A est conforme a la norme NMB 003 du Canada Note This is a class A product
10. Typhoon FLA 9000 User Manual 28 9607 65 AB Requirements Content 1 Introduction 1 2 Regulatory information Identity of person authorized to sign Declaration of Conformity See EC Declaration of Conformity International standards This product fulfills the requirements of the following standards Standard EN 61010 1 IEC 61010 1 UL 61010 1 IEC 61010 2 81 CAN CSA C22 2 No 61010 1 Description Safety requirements for electrical equipment for measurement control and laboratory use Notes EN 61326 1 VCCI Class A FCC Part 15 B Class A ICES 003 Class A EMC emissions and immunity require ments for electrical equipment for measurement control and laboratory use Harmonized with 2004 108 EC EN ISO 12100 1 12100 2 Safety of machinery Basic concepts general principles for design Harmonized with 2006 42 EC EN ISO 14121 1 14121 2 Safety of machinery Principles of risk assessment Harmonized with 2006 42 EC EN 60825 1 IEC 60825 1 Safety of laser products Harmonized with 2006 95 EC USA 21 CFR Chapter I Subchapter CE Conformity Safety of laser products J Part 1040 10 Laser Products This product complies with the European directives listed in the table by fulfilling the corresponding harmonized standards A copy of the Declaration of Conformity is available on request Directive Title 2006 42 EC Machinery Directive
11. aE EEE AA E A E EEN AE A AE A EEE EE E AEA 91 Troues NOOtNO eae Ree Rem tere eee eee fr nr ea eT Se eRe Meee eR Pere Aen Per E epee oe 93 Default sample detection methods coc cccctisscsccess chins wtsacescsizacencasaseesestuescbos dabsodansscinasacdusacorexenctubecemmeabeeenccds 98 SCC i A AEE AE E E A AE ATO OOA TA 101 Typhoon FLA 9000 User Manual 28 9607 65 AB 1 Introduction 1 Introduction In this chapter This chapter contains important user information and a general description of Typhoon FLA 9000 and its intended use 1 1 Important user information Read this before using the Typhoon FLA 9000 All users must read this entire manual to fully understand the safe use of Typhoon FLA 9000 Intended use Typhoon FLA 9000 Fig 1 is a versatile laser scanner for biomolecular imaging applications including the following e sensitive and quantitative measurement of radioisotopic labels e 2 DDIGE e chemifluorescent Western blots ECL Plus e visible single channel and multiplex fluorescence ECL Plex Western blotting systems e near infrared fluorescence e colorimetric stains e g Coomassie blue and silver stained gels Safety notices This user documentation contains WARNINGS CAUTIONS and NOTICES concerning the safe use of Typhoon FLA 9000 See definitions below Warnings WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is impor
12. filter module in the filter module holder 3 Close the filter change door Register the filter change in Typhoon FLA 9000 Control Software Step Action il Click the Filter Module button in the main window Y Fite moduie 54 Typhoon FLA 9000 User Manual 28 9607 65 AB Step 8 Filter module settings 8 4 Installing and replacing filters Action Click the desired filter position on the filter tray gt Insert lt Remove Filter Module Module_2 1 BPG 2 BPB Load Filter Module LPR B 4 B P Save Filter Module Select the filter to be registered from Filter List E Filter Module Settings a Filter List Name BPB1 P BPFR700 P BPFR800 BPG1 E DBR1 W DGR1 E P ch 2 E P E LPB LPG Filter Module Module_2 E LPR ME 1 BPG r 2 E BPB1 Load Filter Module 3 W LPR 4 P Save Filter Module Cancel OK Click the Insert button to register the filter gt Insert TIP You can also drag the filter from Filter List to the desired filter position Click the OK button Typhoon FLA 9000 User Manual 28 9607 65 AB 55 8 Filter module settings 8 5 Using filters from third party suppliers in Typhoon FLA 9000 8 5 Using filters from third party suppliers in Typhoon FLA 9000 Filters from third party suppliers can be used in Typhoon FLA 9000 by using a filter expansion box optional
13. front of the Typhoon FLA 9000 is lit 2 Start Typhoon FLA 9000 Control Software from the Start menu or use the desktop shortcut 3 The main window of the Typhoon FLA 9000 Control Software is displayed Typlwun FLA 9000 Mode Settings Bore Typhoon FLA 9000 System Settings The condition is displayed in the Status area of the main window Status messages are as follows Message Explanation Disconnected Cannot recognize Typhoon FLA 9000 Please check connection and power Warm up Typhoon FLA 9000 is in self diagnosis Please wait Ready The unit is ready to use Check the installed and registered filters Make sure that the filters installed in Typhoon FLA 9000 are registered correctly in Typhoon FLA 9000 Control Software 64 Typhoon FLA 9000 User Manual 28 9607 65 AB 9 Operation 9 3 Starting Typhoon FLA 9000 and Typhoon FLA 9000 Control Software Step Action 1 Click the Filter Module button in the main window Gy Fiter Module A status message appears Wait until the message is closed before proceeding to the next step 2 Note the list of currently registered filters X B ae Load Filter Module 3 LPG 4 LPR _ Save Filter Module 3 Press the button on the filter change door Typhoon FLA 9000 User Manual 28 9607 65 AB 65 9 Operation 9 3 Starting Typhoon FLA 9000 and Typhoon FLA 9000 Control Software Step Action 4 Open the f
14. that may be hazardous Before installing operating or maintaining the equipment you must be aware of the hazards described in the user documentation Follow the instructions provided to avoid personal injury or damage to the equipment 2 2 General precautions WARNING Do not use the equipment if smoke strange noises or strange odors can be perceived or if the equipment becomes unusually hot This may result in fire or electric shock Stop using immediately turn off the power switch and unplug the equipment from the power outlet Contact your local GE Healthcare representative to request repair WARNING Do not damage the power supply cord by bending twisting heating or allowing them to become pinned under the equipment Using damaged power cords could result in fire or electric shock If the power supply cords are damaged contact your local GE Healthcare representative for replacements WARNING Do not place the equipment on unstable tables or on inclined surfaces as the equipment could be dropped or fall resulting in injury Typhoon FLA 9000 User Manual 28 9607 65 AB 13 2 Safety instructions 2 3 Personal protection 2 5 Personal protection A CAUTION Always wear gloves protective glasses and a lab coat or similar when handling samples A CAUTION Always wear cotton gloves when handling storage phosphor screens 2 4 Laser safety WARNING Never detach the inner c
15. the screenshots below the method name given is no name Y9 Typhoon FLA 9000 Ea i Are you sure you want to delete this method no name 5 Finalize the changes by clicking the OK button in the Method Settings dialog box Note To undo the filter deletion click the Cancel button in the Method Settings dialog box Click Yes in the dialog box that appears see below SD Typhoon Fa 9000 Are you pure you mart tn cancel the changes you have made in the method setting halog Coe ote 48 Typhoon FLA 9000 User Manual 28 9607 65 AB 8 Filter module settings 8 Filter module settings Introduction Filter module settings are registered by a serviceman upon installation Under normal circumstances it is not necessary to register these settings When a filter is changed in the filter module of Tyohoon FLA 9000 the new or changed filter must be registered in the Typhoon FLA 9000 Control Software A filter which has not been registered can not be used for analysis and is not displayed in the Typhoon FLA 9000 Control Software 8 1 Registering a new filter name Step Action 1 Click the Filter Module button in the main window U Fiter Module Click the Add button The following dialog box appears Li x Channel ch tonly C ch 2only ch land ch 2 Name none Icon Oo 2 Ce ee Type the filter name in the Name field Select an icon in the Icon drop down list Select w
16. 0 i os gt Location te modi Recent Places E hik E Compete baa Bake 10 Select the USB Control folder and click Open Lock C DVD RW Deve Typhoon FLA 1000 089p n Name Type Date most Location gt Faes Current on the Dise 2 Recent Places USE Control E fe foder cont Ji USB Fecsction tea Typhoon FLA 9000 User Manual 28 9607 65 AB Select Show All Devices and click the Next button Fiom the litt below select the type of hardware you are installing Insert the Typhoon FLA 9000 Control Software CD and click the Browse button Select to install the driver from the Typhoon FLA 9000 Control Software CD 39 5 Installing Typhoon FLA 9000 Control Software 5 3 Install Typhoon FLA 9000 Control Software under Windows Vista Step Action 11 Select the file DevMng and click the Open button Tioti B Lookin ji USE Contr OF Po a e n z ssa 12 Click the OK button in the dialog Install from disk 13 Click the Next button in the wizard Add hardware 14 Click the Next button once again 15 The following warning is displayed Proceed by cliking Install this driver software anyway 16 Click the Finish button in the Add Hardware wizard to complete the installation Install the USB function driver Windows Vista 40 Note Step During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to c
17. 00 Cooling fans The instrument has cooling fans in two places shown below in order to prevent the internal temperature from rising Typhoon FLA 9000 User Manual 28 9607 65 AB 23 3 System configuration 3 2 Instrument body CAUTION Do not block the cooling fans If they are blocked the instrument may malfunction Exhaust fan Air intake fan Indicator lamps Three indicator lamps indicate the instrument status 24 Typhoon FLA 9000 User Manual 28 9607 65 AB 3 System configuration 3 2 Instrument body Stage setting block Door of the stage setting block Stage setting block Filter module The filter module is located behind the filter door Typhoon FLA 9000 User Manual 28 9607 65 AB 25 3 System configuration 3 2 Instrument body USB port The USB port is located on the rear side of Typhoon FLA 9000 USB port 3 5 Accessories Digitization plate included The digitization plate is a fluorescent plate that emits light when exposed to a laser beam This plate is used when the digitization mode is used Fluor stage included The Fluor stage is used to read gels or membranes e Place the gel or membrane directly on the glass of the Fluor stage e Place the Fluor stage in the instrument 26 Typhoon FLA 9000 User Manual 28 9607 65 AB 3 System configuration 3 3 Accessories Glass surface Glass slide holder included When the TP plugin is used
18. 09 E Typhoon FLA 9000 GE Meatthcare A 3 Confirm the uninstallation by clicking Yes when prompted 4 If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified program wants access to your computer Click Allow Upgrading Typhoon FLA 9000 Control Software 44 Step Action 1 Uninstall the current version of Typhoon FLA 9000 Control Software 2 Install the new version of Typhoon FLA 9000 Control Software Follow the instructions in the installation chapter of Getting started with Typhoon FLA 9000 Typhoon FLA 9000 User Manual 28 9607 65 AB 7 Registering editing and deleting methods 7 Registering editing and deleting methods Note Method settings are registered by a serviceman upon installation Under normal circumstances it is not necessary to change these settings 7 1 Registering anew method Step Action 1 Click the Method button on the main window The Method Settings dialog box appears 3 Method settings hhh Method Settings Method List Name Alexa Fluor 488 LPB ch 1 LPG ch 1 Alexa Fluor 555 E LPG ch 1 Alexa Fluor 647 LPR ch 1 Alexa Fluor 680 ch 2 P BPFR700 ch 2 Alexa Fluor 700 ch 2 FJ BPFR700 ch 2 Alexa Fluor 750 ch 2 BD BPFR800 ch 2 Alexa Fluor 790 ch 2 BD BPFR800 ch 2 E LPB ch 1 E LPG ch 1 N Through ch 1 Typhoon FLA 9000 User Manual 28 9607 65 AB 45 7 Registering e
19. AB 59 8 Filter module settings 8 5 Using filters from third party suppliers in Typhoon FLA 9000 Step Action 9 Lift out the mirror holder 9 Insert a mirror 10 Place the mirror holder in the filter expansion box then insert and tighten the screws 11 Attach the cube cover and tighten screws 60 Typhoon FLA 9000 User Manual 28 9607 65 AB 8 Filter module settings 8 5 Using filters from third party suppliers in Typhoon FLA 9000 Step Action 12 Insert the filter holders in the openings of the the filter expansion box and tighten them Typhoon FLA 9000 User Manual 28 9607 65 AB 61 9 Operation 9 Operation 9 1 Operation overview Using Typhoon FLA 9000 comprises a series of steps outlined below Detailed explanations are provided in subsequent chapters Turn on the Typhoon FLA 9000 Phosphorimaging samples Start the computer and launch the Typhoon FLA 9000 Control Software Expose the storage phosphor screen Set the reading parameters Place the stage and the sample in the Typhoon FLA 9000 Read the sample 9 2 Preparations before starting Typhoon FLA 9000 Checklist before starting Typhoon FLA 9000 e Typhoon FLA 9000 is placed on a sturdy horizontal surface e Typhoon FLA 9000 is connected to a grounded wall outlet e The air intake fan on the rear side of Typhoon FLA 9000 is unobstructed e The air exhaust fan on the left side of Typhoon FLA 9000 is unobstructed e There are no objects on
20. GE Healthcare Typhoon FLA 9000 User Manual Table of Contents 1 Introduction Important user information Regulatory information The Typhoon FLA 9000 laser scanner Typhoon FLA 9000 Control Software 1 1 1 2 1 3 1 4 2 Safety instructions atl Eh g eee nonaeeneteete maemo E E General precautions Personal protection LaSer SCANO TY cecossesscecansass acecsspuassncecasascesctesoaassecvcnsanesnatastaraints 2 1 2 2 23 2 4 2 5 2 6 2 7 2 8 2 9 Radiation safety Radiation hazard prevention Electrie Safety isinna Labels on Typhoon FLA 9000 Emergency procedures 2 10 Recycling and disposal 3 System configuration 3 1 3 2 3 3 4 Installing and moving Typhoon FLA 9000 Site requirements Transporting Typhoon FLA 9000 CONNECHO N Sheis 4 1 4 2 4 3 5 Installing Typhoon FLA 9000 Control Software Installation sequence Install Typhoon FLA 9000 Control Software under Windows XP Install Typhoon FLA 9000 Control Software under Windows Vista 5 1 5 2 5 3 6 Uninstalling and upgrading Typhoon FLA 9000 Control Software 7 Registering editing and deleting methods Registering a new method Editing a method Deleting a method 7 1 7 2 7 3 8 Filter module settings Registering a new filter name Saving a filter combination Loading a filter combination Installing and replacing filters Using filters from third party suppliers in Tyohoon FLA 9000 8 1 8 2 8 3 8 4 8 5 Typhoon FLA 9000 User Manual 28 9607 65 AB
21. In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures Laser standards This instrument meets the laser radiation safety requirements specified in the Code of the Federal Regulations 21 CFR Chapter 1 Subchapter J This equipment is a class 1 Laser Product IEC60825 1 2001 EN60825 1 2002 Typhoon FLA 9000 User Manual 28 9607 65 AB GE Healthcare Bio Sciences AB 751 84 Uppsala Sweden FLUORESCENT IMAGE ANALYZER MODEL Typhoon FLA 9000 SERIAL No MANUFACTURED FFTPT Complies with FDA performance standards for laser products except for deviations pursuant to Laser Notice No 50 dated June 24 2007 405N101495 1 Introduction 1 2 Regulatory information Any four out of the five lasers in the following table can be installed in Typhoon FLA 9000 Laser and class Wavelength Maximum power LD laser class3B 473 nm 20 mW CW SHG laser class 3B 532 nm 10 mW CW LD laser class 3B 635 nm 45 mW CW LD laser class 3B 685 nm 55 mW CW LD laser class 3B 785 nm 120 mW CW Regulatory compliance of connected equipment Any equipment connected to the Typhoon FLA 9000 should meet the safety requirements of EN 61010 1 IEC 61010 1 or relevant harmonized standards Within EU connected equipment must be CE marked 1 3 The Typhoon FLA 9000 laser scanner Imaging of gel membrane etc dyed or labelled
22. Meaning This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment This symbol indicates that the product contains hazardous materials in excess of the 0 limits established by the Chinese standard SJ T11363 2006 Requirements for Concen tration Limits for Certain Hazardous Substances in Electronics Labels concerning laser light Label Meaning CAUTION Avoid exposure to the laser beam when the lid is open CAUTION Class 3B Laser product when open and interlock defeated Avoid exposure to the beam Do not attempt to defeat the safety interlocks under the sample lid or behind the filter door or otherwise try to gain access to the interior of the instrument through any other opening Exposure to laser light can cause injury Viewing the laser light directly can cause blindness 18 Typhoon FLA 9000 User Manual 28 9607 65 AB 2 Safety instructions 2 8 Labels on Typhoon FLA 9000 Labels on rear and right side of Typhoon FLA 9000 This device complies with part 15 of the FCC Rules Operation is subject to the following two conditions 1 this device may not cause harmful interference and 2 this device must accept any interference received including interference that may ca
23. Select the type of tone curve used in the intensity graph Setting Description Exponential The light intensities are displayed using an exponential tone curve Linear The light intensities are displayed using a linear tone curve Sigmoid The light intensities are displayed using a sigmoid S shaped tone curve Auto Range Scope Check this option to automatically adjust the range of light intensities for optimum display results Color Select which colors to use to display the data in the display area Setting Description Negative Gray Applies gradation based on white to the Low side and based on black to the High side Positive Gray Applies gradation based on black to the Low side and based on white to the High side Red Applies gradation based on black to the Low side and based on red to the High side Green Applies gradation based on black to the Low side and based on green to the High side Blue Applies gradation based on black to the Low side and based on blue to the High side Color32 Applies 32 gradation steps from blue on the Low side and to red on the High side 84 Typhoon FLA 9000 User Manual 28 9607 65 AB 10 Reading conditions display parameters and other settings 10 3 Other settings 10 3 Other settings Preferences gt Preferences Click the Preferences button in the main window to display a tabbed dialog box where various option
24. The software allows you to use several kinds of filters Items required e Filter expansion box e Rectangular mirror for the filter expansion box e 25mm filter e Small Phillips screwdriver The following procedure describes how to prepare the filter expansion box Step Action it Place the filter expansion box on a stable surface 56 Typhoon FLA 9000 User Manual 28 9607 65 AB 8 Filter module settings 8 5 Using filters from third party suppliers in Typhoon FLA 9000 Step Action 2 Remove the two filter holders by turning them counterclockwise In this image the lower filter holder contains a dummy filter 3 Unscrew and remove the filter rings from the filter holders oO M 0OO In this image the dummy filter is at the bottom left Typhoon FLA 9000 User Manual 28 9607 65 AB 57 8 Filter module settings 8 5 Using filters from third party suppliers in Typhoon FLA 9000 Step Action 4 Place a 25 mm diameter filter in the loading part 5 Place the ring on the filter in the filter holder 6 Unscrew and remove the four small screws on the outside of the cover FAN 58 Typhoon FLA 9000 User Manual 28 9607 65 AB 8 Filter module settings 8 5 Using filters from third party suppliers in Typhoon FLA 9000 Step Action 7 Pull off the cover and place it to one side sz 2 b 8 Unscrew and remove the two screws fixing the mirror and mirror holder Typhoon FLA 9000 User Manual 28 9607 65
25. allation by clicking Yes when prompted Note Correction files created during calibration such as shading data are required by the Typhoon FLA 9000 Control Software They are not deleted during the uninstallation and remain in the Data folder of Typhoon FLA 9000 Control Software folder Uninstalling Typhoon FLA 9000 Control Software under Windows Vista Step Action 1 Open the control panel and Select Uninstall a program under Programs Typhoon FLA 9000 User Manual 28 9607 65 AB 43 6 Uninstalling and upgrading Typhoon FLA 9000 Control Software Step Action 2 Select Typhoon FLA 9000 and then click Uninstall O Control Panel Programs Programa and Features z Seah z 2 Uninstall or change a program To uninstall program elect it feom the list and then click Uninstall Change or Repair Organise ff Views Uwian E Change Eh Repair Name d Publisher Installed On R DeCyder 2D Database GE Heatthcare 6 20 2009 dec yer 20 Oracte Server GE Heahhcare 6n D DeCyder 2D Program GE Healthcare 6 10 2009 cbjimageManter 20 Platinum 7 GE Meattncare 6 0 2000 ImageQuant GE Healthcare 602009 B rHediR Graphics Media Accelerator Driver Intel Corporation 60 2009 PAR Network Connections 134 331 Intel 6 10 2009 bei TY Missed Intel Corporation ame LT Secutfty GE Healthcare 6 10 2009 PB Microso NET Framework 14 Merosot Em 1D Mictosett NET Framework 3 5 SPL Microsoft Comeration 6 10 20
26. com quantitative_imaging imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company Typhoon is a trademark of GE Healthcare companies All third party trademarks are the property of their respective owners 2009 General Electric Company All rights reserved First published Oct 2009 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9607 65 AB 12 2009
27. diting and deleting methods 7 1 Registering a new method Step Action 2 Click the Add button The following dialog box appears E Edit Method Channel 1 Name reagent C Channel 2 Name Laser 1 Bj sHaa73 Laser 2 WB Erc cht Filter Tick the checkboxes next to Channel 1 or Channel 2 to select a PMT photomultiplier tube to use Select both channels when using a two channel filter 4 Enter a name for the combination in the Name field 5 Select the type of laser in the Laser 1 or Laser 2 drop down lists You may select lasers which are not currently loaded 6 Select the type of filter in the Filter drop down list You may select a filter which is not currently loaded f Click the OK button The list in the Method Settings window now contains the new method with the selected laser and filter combination Note Methods containing lasers or filters that are not currently loaded cannot be selected in the Reader Condition screen 46 Typhoon FLA 9000 User Manual 28 9607 65 AB 7 Registering editing and deleting methods 7 2 Editing a method 7 2 Editing a method Step Action 1 Click the Method button in the main window The Method Settings dialog box appears 38 Method Settings EEE Method Settings Method List Alexa Fluor 488 Alexa Fluor 532 LPG ch 1 Alexa Fluor 555 E LPG ch 1 Alexa Fluor 647 LPR ch 1
28. ds or multiple loads on one electrical outlet could result in fire and electric shock CAUTION Do not use the same power supply as that of large equipment such as an air conditioner or centrifuge Malfunction could result Typhoon FLA 9000 User Manual 28 9607 65 AB 2 Safety instructions 2 8 Labels on Typhoon FLA 9000 2 8 Labels on Typhoon FLA 9000 Typhoon FLA 9000 serial number The Typhoon FLA 9000 serial number is located on a label on the back of the instrument GE Healthcare Bio Sciences AB 751 84 Uppsala Sweden FLUORESCENT IMAGE ANALYZER Model Typhoon FLA 9000 Serial No 123456 Frequency 50 60 Hz Supply Voltage 100 240 V c US Max Current 3 0A 1 5A STED Protection Class IP20 Manufacturing Year ACERS Marutacturer FUJIFILM Corporation TOKYO 106 8620 JAPAN MADE IN JAPAN 405N101494 LABORATORY EQUIPMENT 19NS Symbols used in safety labels Label Meaning Warning Read the user documentation before using the system Do not open any covers or replace parts unless specifically stated in the user documentation The system complies with the requirements for electromagnetic compliance EMC in Australia and New Zealand C The system complies with applicable European directives Typhoon FLA 9000 User Manual 28 9607 65 AB 17 2 Safety instructions 2 8 Labels on Typhoon FLA 9000 Labels concerning use of hazardous substances Label
29. ect Typhoon FLA 9000 Please check The scanner is not detected connection and power Countermeasure Check that the scanner is turned on and connected to the computer Typhoon FLA 9000 User Manual 28 9607 65 AB 93 13 Troubleshooting Error message Meaning and countermeasure Error Please restart Typhoon FLA 9000 The scanner needs to be restarted Countermeasure Switch of the power button of Typhoon FLA 9000 wait 15 seconds and switch it on again The system is not for phosphorimaging Rec The current scanner settings are inappropriate ommended method for phosphorimaging for the current scanning mode The laser and or Phosphor 635 nm IP filter settings do not match the selected scanning SEPET mode The system is not for digitization Recommend ed methods for digitization CCB 532 nm Countermeasure Make sure that the correct laser LPG Silver Stain 473 nm LPB and filter combination is installed in the scanner If necessary change the filter Follow the instruc The system is not for chemiluminescence tions in Section 8 4 Installing and replacing filters Method for chemiluminescence Chemilumines on page 51 Click the Method button and select cence no lasers Through a different method If the required laser is not installed contact your GE Healthcare representative No methods are available No method is defined Countermeasure Click the Method button and create a met
30. eement is not acceptable please contact a GE Healthcare representative See the back cover of this manual for contact information Select accept the terms in the license agreement and click the Next button 5 Select destination folder in the dialog DATE e Click the Next button to install the software at the default folder C Program Files e Click the Change button to install to a different folder 6 Click the Install button Typhoon FLA 9000 User Manual 28 9607 65 AB 41 5 Installing Typhoon FLA 9000 Control Software 5 3 Install Typhoon FLA 9000 Control Software under Windows Vista Step Action 7 If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified program wants access to your computer Click Allow 8 Click the Finish button The installation of Typhoon FLA 9000 Control Software is now completed 42 Typhoon FLA 9000 User Manual 28 9607 65 AB 6 Uninstalling and upgrading Typhoon FLA 9000 Control Software 6 Uninstalling and upgrading Typhoon FLA 9000 Control Software Before you begin Log in using a Windows account with administrator privileges Uninstalling Typhoon FLA 9000 Control Software under Windows XP Step Action il Open the control panel and select Add or Remove Programs 2 Select Typhoon FLA 9000 and click the Remove button astdecur GLIU iastdec cr WLL 3x PAIR x BP 53u le Lypcebyler Yina 4 KBSZEFES 3 Confirm the uninst
31. es e Set the position of the lower left corner by changing the list box values in the row Lower Left e Set the position of the upper right coner by changing the list box values in the row Upper Right Number Asubmenu with eligible numbers for scanning areas Select a number to assign it to the scanning area Note Changing the number of one scanning area renumbers all other scanning areas as well Enabled Check this box to enable the scanning area Uncheck the box to disable the scanning area File Size This area displays the estimated size of the image file as determined by the current settings Reading Time This area displays the estimated reading time as determined by the current settings 82 Typhoon FLA 9000 User Manual 28 9607 65 AB 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Prescan button not available in phosphorimaging mode Click this button to perform a quick scan at a resolution of 1000 um Use the prescan feature to optimize the PMT settings for the signal intensity of the current sample Prescanning the sample is quicker than performing a regular scan especially if the pixel size is small Scan button Click this button to start scanning the sample or samples using the current reading conditions 10 2 Display parameters explained Beanery progress File Name test_reading CBB Amare tore
32. etected by the com puter In the Found New Hardware Wizard dialog choose No not this time Welcome la The Found New llardware Wizard Click the Next button in the Found New Hardware Wizard dialog Insert the installation CD Select Install the software automatically Recommended Click the Next button in the Found New Hardware Wizard dialog Click the Finish button to complete the installation Install Typhoon FLA 9000 Control Software Windows XP Step Action 1 2 3 Insert the Typhoon FLA 9000 Control Software CD Locate and double click the file Typhoon FLA 9000 msi In the Typhoon FLA 9000 InstallShield Wizard click the Next button Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative see back cover of this manual for contact information Select I accept the terms in the license agreement and click the Next button Typhoon FLA 9000 User Manual 28 9607 65 AB 37 5 Installing Typhoon FLA 9000 Control Software 5 2 Install Typhoon FLA 9000 Control Software under Windows XP Step Action 5 Select destination folder in the dialog IS lyjilmnan H A BUKK reiba IS lrir hal Wiren at Drstination Fairer Sbalre hve thteey cede Tanhai vette wits oy Ital oproer TLA 2093 to Aae ec e Click the Next button to install the software at the default folder C Program Files e Click the Change button to install to a different folder 6 Clic
33. evel Initial delivery inspection Upon receiving Typhoon FLA 9000 e inspect the package for external damages e check that all items in the packaging list are included Should you find any external damages or if any items on the packaging list are missing notify the delivery company and contact GE Healthcare for further advice Store Typhoon FLA 9000 in an environment according to Appendix B Specifications on page 101 until the product is unpacked and installed Typhoon FLA 9000 User Manual 28 9607 65 AB 31 4 Installing and moving Typhoon FLA 9000 4 2 Transporting Typhoon FLA 9000 4 2 Transporting Typhoon FLA 9000 CAUTION Typhoon FLA 9000 must be secured before long distance transports Contact GE Healthcare for help and advice before transporting Typhoon FLA 9000 long distances The Typhoon FLA 9000 weighs approximately 97 kg and requires at least two persons to lift and move Precautions before moving the instrument Step Action 1 Turn off the Typhoon FLA 9000 2 Turn off the computer and any peripheral devices 3 Disconnect the power cords and the USB connection 4 3 Connections CAUTION Only authorized service personnel is allowed to install Typhoon FLA 9000 Contact your local GE Healthcare representative for help and advice Communication Step Action 1 Connect a USB cable to the USB port on the rear side of the Tyohoon FLA 9000 2 Connect the other end of the USB cable t
34. ext button in Add hardware wizard 5 Select Yes I have already connected the hardware and click the Next button 34 Typhoon FLA 9000 User Manual 28 9607 65 AB Step 5 Installing Typhoon FLA 9000 Control Software 5 2 Install Typhoon FLA 9000 Control Software under Windows XP Action 10 11 Select Add a new hardware device and click the Next button dalil Haalari Wize Thy fulluwiny headway e aiualy indad uri yuu computer art tina yter mh vee wrae ei ark mgn te elindacrala mibo hery Te sddhadvare vtehewir riw acd aren wrewes derie sealed be duee Sy 60 Tokit eassa Jon Gg iw Hih carmel hear Jei Select Install the hardware that I manually select from a list Advanced and click the Next button Select Show All Devices and click the Next button dubil Haalari Wirit From tis livi beduw pulusi Hu ype of hadia puu ave inelaliriy ya cand see He babi gdes y paawa due shoe Mene Terre g8icna s pes Click the Have Disk button in the Add hardware wizard Insert the Typhoon FLA 9000 Control Software CD and click the Browse button Select to install the driver from the Typhoon FLA 9000 Control Software CD Lacate Lile ork j H be Sores E Zort l ee Loca B kieg t Jocumerile Saua ETES Se STCRI R u erp Ds oc lt STCFENGO E OS aedCuwu eds D My Dozunerts Sy He atok Places Typhoon FLA 9000 User Manual 28 9607 65 AB 35 5 Installing Typhoon FLA 9000 Control Sof
35. gs window Error message Meaning and countermeasure Please input a method name No name has been assigned to the method Countermeasure Type a method name in the Name field The maximum number of available methods is 100 The maximum number of methods are stored No more methods can be registered Countermeasure Use an existing method or delete a method before registering a new one This method name has been already used Please change the name The assigned name is being used by a different method Countermeasure Type a different name in the Name field Errors and warnings in the Reader Settings window Error message Meaning and countermeasure Typhoon FLA 9000 imager is running self diagnosis mode Please wait The self diagnosis of Typhoon FLA 9000 is running Countermeasure Wait until the self diagnosis is finished the proceed with the scan Typhoon FLA 9000 is not ready to scan Please wait a moment and try again The scanner is not ready to scan Countermeasure Wait for one minute then try to scan again A laser error was detected Use oth er lasers There was an error with the laser Countermeasure Try to scan again Countermeasure Restart the instrument and Typhoon FLA 9000 Control Software then try to run the scan again Countermeasure Scan using a different laser Typhoon FLA 9000 User Manual 28 9607 65 AB 95 13 Troubleshooti
36. hich PMT to use e ch 1 only Only the first PMT will be used e ch 2 only Only the second PMT will be used e ch 1 and ch 2 Both PMTs will be used Finalize the filter addition by clicking the OK button Typhoon FLA 9000 User Manual 28 9607 65 AB 49 8 Filter module settings 8 1 Registering a new filter name Step Action 7 Click the OK button to return to the main window 8 2 Saving a filter combination Step Action 1 Click the Filter Module button in the main window UY Fitter Module 2 Click the Save Filter Module button 3 Type a name for the filter combination in the File name field 4 Click the OK button 5 Click the OK button to return to the main window 8 3 Loading a filter combination Step Action 1 Click the Filter Module button in the main window U Fitter Module 2 Click the Load Filter Module button 3 Select a filter module to load in the Load Filter Module dialog box 4 Click the OK button 5 Click the OK button to return to the main window CAUTION When removing the filter module make sure to click the Filter Module button and remove the filter module after the window changes to the Filter Settings window If the filter module is forcefully removed the area where the filter comes in contact with the PMTs may be damaged 50 Typhoon FLA 9000 User Manual 28 9607 65 AB 8 Filter module settings 8 4 Installing and replacing filters 8 4 In
37. hod to use for scanning Errors and warnings in the Filter Module Settings window Error message Meaning and countermeasure Please enter a filter name The filter has no name Countermeasure Type a name in the Name field The filter name has already been The name of the filter is being used for another filter used Please change the name Countermeasure Choose another name for this filter or change the name for the filter already having this name The maximum number of the filters No more filters can be stored in the software you can register is 50 Please delete an unnecessary filter before regis teringa a new filter Countermeasure Delete a filter before storing a new one The filter is in use and cannot be The filter is being used and can therefore not be deleted deleted Countermeasure Select a different filter to use before deleting the current one 94 Typhoon FLA 9000 User Manual 28 9607 65 AB Error message 13 Troubleshooting Meaning and countermeasure No methods are available No methods are available for scanning Countermeasure Register new methods in the Typhoon FLA 9000 Control Software The filter module is currently in use and cannot be deleted The filter module is being used and can therefore not be deleted Countermeasure Select a different filter module to use before deleting the current one Errors and warnings in the Method Settin
38. ilter change door by pulling the knob 5 Check that the set of currently installed filters matches the set of filters noted in step 2 If the registered and installed filter sets match e Close the filter module door e Click the OK button in the Filter Module Settings window If the registered and installed filter sets DO NOT MATCH e Change the installed or registered filters as required e Close the filter module door e Click the OK button in the Filter Module Settings window 9 4 Selecting the reading mode Click the button that corresponds to the desired reading mode 66 Typhoon FLA 9000 User Manual 28 9607 65 AB 9 Operation 9 4 Selecting the reading mode To Click this button Read a fluorescent sample Read a chemiluminescent sample c Read a phosphorimaging sample AA Read a digitization sample EF oean Note To use the chemiluminescence mode at least one filter s type must be set to Through If necessary set a filter to Through following the instructions in Section 8 3 Loading a filter combination on page 50 9 5 Setting the reading conditions Set the reading conditions by following the instructions in Chapter 10 Reading conditions display parameters and other settings on page 77 9 6 Placing the object to be scanned on the stage CAUTION Always wear gloves protective glasses and a lab coat or similar when handling samples Placing a gel sample on the Flu
39. k the Install button in the installation dialog 7 Click the Finish button to finish the installation of Tyohoon FLA 9000 Control Software 5 3 Install Typhoon FLA 9000 Control Software under Windows Vista Before you begin Log in using a Windows account with administrator privileges Install the USB Control Driver Windows Vista Note During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Step Action 1 Disconnect Typhoon FLA 9000 Control Software from the computer 2 Open the control panel and click Classic View in the upper left corner 3 Open Add Hardware 4 In the Add Hardware dialog click the Next button 5 Select Install the hardware that I manually select from a list Advanced and click the Next button 38 Typhoon FLA 9000 User Manual 28 9607 65 AB Step Action 5 Installing Typhoon FLA 9000 Control Software 5 3 Install Typhoon FLA 9000 Control Software under Windows Vista 6 Wt you do net see the hardware category you want chick Show Al Devices Common hardware typer By Digi aerer CP IDE ATAVATAPI coensoiers ig gt IEEE 1394 Bus host conwolers Imaging devers apep devces Maka Faim E nianie Gee teeta Laa 7 Click the Have Disk button Lock G DVO AW Deve Typhoon FLA S00
40. lay parameters and other settings 10 1 Reading conditions explained Save condition Click this button to save the current conditions settings to a file Save the current conditions by clicking Save Condition A text dialog opens Enter a name for the conditions in the text field and click OK to save Load condition Pixel size Click this button to load previously saved conditions Clicking the button opens a dialog box with a list of previously saved condition sets Load a set of stored conditions by selecting it in the list then click OK Remove a set of stored conditions by selecting it then click Remove Confirm the removal by clicking the Yes button in the next dialog box PRAVIA Set the pixel size for reading O23 um O80 pm C100 pm 200 pm l O10 um Click to select from one of the five types A sample with a smaller pixel size is analyzed in finer detail 200 100 50 25 10um lt lt Short Reading Time Long lt 7 Small Image File Size Large The 10 um pixel size processing is conducted by applying Bi cubic algorithm to the images read at 25 um pixel size Therefore computer processing after reading may take a longer time than image reading time Bi cubic Using the densities of 16 lattice points around Up Vo cubic interpolation is done f uo vo X f ux ui c ux uo vivo k Here u vi is a lattice point around Up Vo and interpolative coefficient c x is defined linear
41. le name in the File name field 3 Select a file format in the Save as type drop down list 4 Save the image file by clicking the Save button 9 12 Viewing the image file in the analysis application Click the Launch button in the to open the image file in the registered analysis application Note If no analysis application is registered in Typhoon FLA 9000 Control Software the Launch button is greyed out and inactive If desired register or change the analysis application 9 13 Turning off Typhoon FLA 9000 Step Action 1 Turn off Typhoon FLA 9000 by pressing the power switch on the right side of the instrument body 76 Typhoon FLA 9000 User Manual 28 9607 65 AB 10 Reading conditions display parameters and other settings 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Save condition Load condition File Name z c Pixel size omment gs Fluorescence 7 o n ia a o Pixel Size wa ma r Cpm gpm FJ um 200 pm Method ees oe vomm 19 pm Area UF Si en E an i a i Area File Size Reading Time Prescan Start Scan Image folder Specify where to save the file after the reading Note When reading more than once and reading of 1ch 2ch is implemented simultaneously a folder with a name set by File Name is created automatically at the specified position and the image data will be saved in the folder File Name Enter the name of a fi
42. le to save image data This field is mandatory in order to start reading a sample Note The method name is automatically added to the specified name and is saved to a file Comment Enter an optional comment The comment is embedded in the file where the image is saved and can be viewed with the analyzing software Typhoon FLA 9000 User Manual 28 9607 65 AB 77 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Method Set the method to use in the scan Method Chemiluminescence w From the pull down menu select the method that corre rae Ss sponds with the sample The selected laser and filter com Alexa Fluor 532 j bination is displayed on the right PMT Alexa Fluor 555 Alexa Fluor 647 E E 00a Phosphate In fluorescent mode up to 4 scans can be performed in a row all with individual methods PMT n You may set the voltage to be applied to the photo multi PMT V 250 1000 plier tube PMT as an integral value within the predeter mined range The larger the value is the higher the sensi tivity 250V Value 1000V Small lt Large Low lt High Sensitivity Plus and minus buttons fluorescent mode only Click to increase the number of scans Click to decrease the number of scans Top button Click this button to return to the main window 78 Typhoon FLA 9000 User Manual 28 9607 65 AB 10 Reading conditions disp
43. ltiplier tube The higher the value the higher the sensitivity The following buttons are available only in fluorescence mode Click to increase the number of scans Click to decrease the number of scans Set the scanning area 1 Selecta method in the drop down menu 2 Drag the red square to the desired position of the scanning area 3 Drag the sides of the red square as needed to adjust the size of the scanning area Pixel Size set the pixel size Choose a small pixel size for high quality images Note that a small pixel size setting increases the reading time and the size of the image file Save Condition click this button to save the current reading conditions in a file if desired For details refer to the User Manual Load Condition click this button to load previously saved reading conditions if desired For details refer to the User Manual 10 File Size the estimated size of the result data file is presented 11 Reading Time the estimated time required for the scan is presented 12 Top return to the main window 13 Start Scan start the scan The sample must be loaded before starting a scan 14 Prescan perform a quick scan at a resolution of 1000 um 12 Typhoon FLA 9000 User Manual 28 9607 65 AB 2 Safety instructions 2 Safety instructions 2 1 Introduction The Typhoon FLA 9000 is powered by mains voltage and is used to image samples
44. luted by radioisotope exceeds the density determined by the Director General Limit of superficial pollution Paragraph 3 of Article 4 of Notice No 15 of the Science and Technology Agency that determines the quantity etc of radiating isotope specifies that the density of radioisotope on the surface polluted by radioisotope must be one tenth of the density defined in Article 8 Article 8 and Table 3 of this Notice define the limits as shown below 1 Superficial density of radioisotope that radiates alpha rays 4 Bq cm 2 Superficial density of radioisotope that does not radiate alpha rays 40 Bq cm Installation site of instrument This instrument is capable of reading not only Storage phosphor screens but also fluorescent pigment label samples non RI method Therefore it is recommended that the user should install it outside the controlled area and use Rl indicated samples without contacting them with Storage phosphor screens directly However as described above the Storage phosphor screen surface may be polluted by radioisotope RI depending on the sample condition since the instrument sticks the sample to the H compatible Storage phosphor screen surface and exposes it in an auto radiography experiment of the H label sample When a sample is in direct contact with a Storage phosphor screen it is generally known that the sample for making an auto radiogram contains a relatively small quantity of radioisotope However the degree
45. ly 1 2 x xP O lt x lt 1 c x 4 8 px 45 hk 1 lt i lt 2 0 2 lt x Function clx is a piecewise three dimensional polynomial approximation of function sinx x that is valid for the sampling theorem for continous signals Typhoon FLA 9000 User Manual 28 9607 65 AB 79 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Area Select the stage which will be used in the scan The scanning area below the list box changes to reflect the selection Scanning area Displays the area or areas to be scanned as red boxes A grey box indicates a scanning area that has been disabled A disabled scanning area will be omitted from scanning until it is enabled again e Change the size of a scanning area Click and drag the edges of the red box with the mouse e Change the position of a scanning area Click and drag the red box with the mouse e Adda new scanning area Click an empty area on the image of the stage or titer plate plugin e Delete a scanning area Select the scanning area and press the Delete key on the computer keyboard Right click a scanning area to display a menu with additional settings w Enabled Duplicate Renumber Menu choice Description Enabled Enable or disable the individual scanning area A tick mark indicates that the scanning area is enabled and will be included in the scan Note A disabled scanning area is omitted from the next scan b
46. merald488 510 520 LPB Qdot 605 603 LPG Qdot 655 655 LPR Qdot 705 702 LPR Qdot 800 792 LPR SYBR Gold 495 537 LPB SYBR Green 494 521 LPB SYBR Green Il 492 513 LPB SYBR Safe 502 530 LPB SYPRO Orange 472 570 LPB SYPRO Ruby 450 610 LPG SYPRO Orange 490 640 LPG 98 Typhoon FLA 9000 User Manual 28 9607 65 AB A Default sample detection methods 532 nm laser Reagentname Excitation wavelength Emission light wavelength Filter Alexa Fluor 53X 532 554 LPG Alexa Fluor 555 553 568 LPG Cy3 550 570 LPG DGR1 BPG1 Deep Purple 528 591 LPG DY 520XL 520 664 LPR EtBR 518 605 LPG HEX 535 553 LPG HNPP 550 562 LPG Pro Q Diamond 555 580 LPG RITC 554 577 LPG ROX 535 567 LPG SYPRO Red 547 631 LPR TAMRA 542 568 LPG 635 nm laser Reagent name Excitation wavelength Emission light wavelength Filter Alexa Fluor 647 653 669 LPR Cy5 649 670 LPR DBR1 DGR1 DDAO Phosphate 634 665 LPR DY 635 647 671 LPR 685 nm laser Reagent name Excitation wavelength Emission light wavelength Filter Alexa Fluor 680 679 702 BPFR700 Alexa Fluor 700 689 700 BPFR700 Cy5 5 675 694 BPFR700 DY 676 674 699 BPFR700 Typhoon FLA 9000 User Manual 28 9607 65 AB 99 A Default sample detection methods Reagent name Excitation wavelength Emission light wavelength Filter DY 682 690 709 BPFR700 IRDye
47. n to return to the Reader Settings screen After the prescan adjust the PMT as required to improve the results of the regular scan 9 9 Scanning the sample Click the Start Scan button to start reading the sample The scanning progress window opens 74 Typhoon FLA 9000 User Manual 28 9607 65 AB 9 Operation 9 9 Scanning the sample Note If you click the Stop button during reading the part that has not been read yet will be saved as an image with a data value of 0 light intensity of 0 Note When you click the Stop button the reading is aborted You cannot start reading again from the location where reading stopped 9 10 Adjusting the display parameters Step Action 1 Adjust the display parameters if desired Follow the instructions in Section 10 2 Display parameters explained on page 83 2 Click Return to return to the Reader Settings screen Typhoon FLA 9000 User Manual 28 9607 65 AB 75 9 Operation 9 11 Saving the image data using a different file name 9 11 Saving the image data using a different file name Step Action 1 After scanning the sample click the Save as button The following dialog opens LT ax Save in S Data gt age Sampling Area ShadingData test_reading dir readingdata Chemiluminescence gel E test_reading CB8 gel E test_reading Cy5 gel File name z Save as type Gel Image File gel Gel Image File gel te 2 Type a fi
48. ng Error message Meaning and countermeasure Please select image folder No image folder is selected Countermeasure Select an image folder by clicking the Browse button and navigating to a suitable folder Please input a file name No name has been assigned to the image file Countermeasure Type a name in the File Name field Please set PMT Voltage value to 250 1000 The PMT voltage setting is outside the permitted range Countermeasure Type a PMT voltage value between 250 and 1000 in the PMT field Please give a name to the condition No name was given to a condition before saving Countermeasure Type a name in the Condition Name field The stage is not properly inserted Please insert the stage in its correct position The stage is not properly inserted Countermeasure Insert the stage properly Errors and warnings in the scan progress window Symptom No image data was scanned Meaning and countermeasure The sets of registered and installed filters do not match The bands in the image are very weak Countermeasure Change installed filters or register other filters in Typhoon FLA 9000 Control Software as necessary CIEE 0 posure Set a lower PMT voltage The scan was stopped because of overex Meaning and countermeasure The scanned image was overexposed due to a high PMT voltage setting Countermeasure Decrease the PMT voltage in the Reade
49. niotelala 2 ws Power cord included Typhoon FLA 9000 uses a special AC power cable Be sure to use power cables specified in service manuals or by service personnel Suction rod included The suction rod is used to lift up or place the the storage phosphor screen on the phosphor stage Press the suction rod against the storage phosphor screen Place a finger on the end of the suction rod then lift up the storage phosphor screen Release the finger to release the storage phosphor screen Q 2 Suction rod Titer plate TP plugin included The TP plugin is used when reading titer plate samples ELEA Typhoon FLA 9000 User Manual 28 9607 65 AB 29 3 System configuration 3 3 Accessories USB cable included The USB cable is used to connect Typhoon FLA 9000 to a computer 30 Typhoon FLA 9000 User Manual 28 9607 65 AB 4 Installing and moving Typhoon FLA 9000 4 Installing and moving Typhoon FLA 9000 CAUTION Only authorized service personnel is allowed to install Tyohoon FLA 9000 Contact your local GE Healthcare representative for help and advice 4 1 Site requirements Note The Typhoon FLA 9000 is intended for indoor use only Power supply 100 to 240 V AC 3 0 to 1 5 A Line frequency 50 to 60 Hz Placement Stable horizontal surface out of direct sunlight Ambient temperature 10 to 30 C Humidity 30 to 70 non condensing Maximum altitude 2000 m above sea l
50. o a USB port on the computer CAUTION e Do not connect any USB devices other than the Typhoon FLA 9000 to the computer in which the Typhoon FLA 9000 Control Software is installed e Use only the Typhoon FLA 9000 Control Software during reading 32 Typhoon FLA 9000 User Manual 28 9607 65 AB 4 Installing and moving Typhoon FLA 9000 4 3 Connections Electrical power Connect the power cord of the Typhoon FLA 9000 to a grounded power outlet WARNING Use only power cords delivered or approved by GE Healthcare Typhoon FLA 9000 User Manual 28 9607 65 AB 33 5 Installing Typhoon FLA 9000 Control Software 5 Installing Typhoon FLA 9000 Control Software Administrator privileges required All tasks related to software installation or uninstallation require a computer account with administrator privileges 5 1 Installation sequence Software installation is performed in the following sequence 1 Install the USB control driver 2 Install the USB function driver 3 Install the Typhoon FLA 9000 Control Software 5 2 Install Typhoon FLA 9000 Control Software under Windows AP Before you begin Log in using a Windows account with administrator privileges Install the USB Control Driver Windows XP Step Action 1 Disconnect Typhoon FLA 9000 from the computer 2 Open the control panel and select Printers and Other Hardware 3 Click Add Hardware to open Add hardware wizard 4 Click the N
51. of superficial pollution of the Storage phosphor screen is greatly influenced by the dryness of the sample and dose of radioisotope in an experiment and may exceed the limits mentioned in Limit of superficial pollution on page 15 When the instrument reads a Storage phosphor screen with a polluted non exposure area it may be polluted The degree of such superficial pollution greatly differs with users operation conditions Superficial pollution may exceed the limit mentioned above Note As mentioned above install this instrument in the RI controlled area if the user uses Rl indicated samples that will be in direct contact with Storage phosphor screens Removal from the controlled area If it is necessary to move the instrument and its laboratory which were installed and have been used in the controlled area from the controlled area make sure that the degree of the superficial pollution is below the limits mentioned in Limit of superficial pollution above Typhoon FLA 9000 User Manual 28 9607 65 AB 15 2 Safety instructions 2 7 Electric safety 2 7 Electric safety WARNING Do not use the equipment with a power supply other than that recommended Fire and electric shock could result WARNING Do not use the equipment within or near a sink or in humid or dusty environments Fire and electric shock could result WARNING Connect the power supply directly to a grounded wall power outlet The use of extension cor
52. on the Multi stage place the sample glasses in the glass slide holder LF glass plate stage included The LF glass plate stage is used to read one or two DIGE gels Place the gel with glass directly on the stage z Aalriciolelrflalyt ityixkiLiminlolpiola Typhoon FLA 9000 User Manual 28 9607 65 AB 27 3 System configuration 3 3 Accessories Membrane weight included The membrane weight is used to hold thin fluorescent samples flat against the Fluor stage gt S Multi stage included The Multi stage is used to read gels with glass gel merely supported by glass or titer plates e Place the gel with the glass plate directly on the Multi stage or place a titer plate on the titer plate plugin attached to the instrument e Place the titer plate on the Multi stage Phosphor stage included The phosphor stage is used when reading storage phosphor screens The stage is magnetic to hold storage phosphor screens e Place the phosphor stage upside down on a flat surface e Place the storage phosphor screen on the phosphor stage with the reading surface facing away from the phosphor stage e Flip the phosphor stage over e Place the phosphor stage in Typhoon FLA 9000 28 Typhoon FLA 9000 User Manual 28 9607 65 AB 3 System configuration 3 3 Accessories evita pip LL EO EXE TIMING OL sy Oyu e a z a a o w vj ealsicipletrlatnliltulxiitimi
53. ontinue Enter an administrator password if prompted then click Continue to proceed with the installation Action Connect the computer and the Typhoon FLA 9000 with a USB cable and turn ON the power switch of Typhoon FLA 9000 Result The scanner will automatically be detected by the computer and the Plug and Play function in Windows Vista starts In the Found New Hardware dialog select Locate and install driver software recom mended Insert the Typhoon FLA 9000 Control Software CD when prompted and click the Next button in the Found New Hardware dialog Typhoon FLA 9000 User Manual 28 9607 65 AB 5 Installing Typhoon FLA 9000 Control Software 5 3 Install Typhoon FLA 9000 Control Software under Windows Vista Step Action 4 Select Install this driver software anyway v See detnite 5 A successful installation message appears Click the Close button Install Typhoon FLA 9000 Control Software Windows Vista Note During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if orompted then click Continue to proceed with the installation Step Action il Insert the Typhoon FLA 9000 Control Software CD 2 Locate and double click the file Typhoon FLA 9000 msi 3 In the Typhoon FLA 9000 InstallShield Wizard dialog click the Next button 4 Read the license text If the license agr
54. or stage Note The sample must not be thicker than 30 mm For solutions the maximum height is 4mm e Make sure there are no bubbles or gaps on the contact surface Typhoon FLA 9000 User Manual 28 9607 65 AB 67 9 Operation 9 6 Placing the object to be scanned on the stage Step Action 1 Place the sample on the Fluor stage 2 Digitization mode only Place the fluorescent plate for digitization on top of the sample Placing a titer plate on the Multi stage Step Action 1 Place the titer plate frame on the Multi stage Al tciotetriatinui tislatelwetwietlebata 68 Typhoon FLA 9000 User Manual 28 9607 65 AB 9 Operation 9 6 Placing the object to be scanned on the stage Step Action 2 Place the titer plate in the desired position in the titer plate frame Placing a DIGE gel on the LF glass plate stage Step Action 1 Place one or two DIGE gels on the LF glass plate stage SS Afatctotetrictintiuts om Dre ne renee 2 Fasten each glass with the front and rear glass holders 3 Press down each glass holder and tighten the screw Placing a gel sample with glass on the Multi stage Step Action 1 Place a gel sample with glass on the Multi stage Typhoon FLA 9000 User Manual 28 9607 65 AB 69 9 Operation 9 6 Placing the object to be scanned on the stage Step Action 2 Carefully fold down the spring loaded glass holders on the glass Placing a chemiluminescent
55. over screwed to this instrument If it is detached laser beam may leak with a risk of loss of vision A A CAUTION Using procedures or adjustments other than those specified in this manual may result in hazardous exposure to laser radiation 2 5 Radiation safety Radiation safety This instrument is not equipped with any radioisotope RI or radiation generating unit and is therefore not regulated by radiation hazard prevention laws However the instrument is capable of reading Storage phosphor screens which may be polluted by radioisotopes RI A CAUTION If radioisotope RI pollution occurs stop use of the instrument immediately and follow the instructions of your radiation administrator Typhoon FLA 9000 User Manual 28 9607 65 AB 2 Safety instructions 2 6 Radiation hazard prevention 2 6 Radiation hazard prevention Controlled area Paragraph 1 of Article 1 of the Law Enforcement Rules for Prevention of Radiation Hazards due to Radioisotope and so forth Prime Minister s Office ordinance No 56 defines the controlled area as a place where the dose equivalent related to external radiation exceeds the dose equivalent determined by the Director General of the Science and Technology Agency hereinafter referred to as the Director General the concentration of radioisotope in the air exceeds the concentration determined by the Director General or the radioisotope density on the surface pol
56. r Settings window opened The scan stopped because the door was The door was opened during the scan Countermeasure Close the door and scan the image again Do not open the door until the scanning is fin ished and filter The combination of the laser and filter might be inappropriate Check the laser The selected method may be inappropriate Countermeasure Select another method or edit the method to suit the current application 96 Typhoon FLA 9000 User Manual 28 9607 65 AB 13 Troubleshooting Error message Meaning and countermeasure A scanner error was detected Please restart Typhoon FLA 9000 imager and Scanner Control Software Sense Key xxxx Error Code yyyy An error occurred during scanning Countermeasure Try to run the scan again Contact your GE Healthcare representative if the problem persists A warning occurred during the scanning process See above Typhoon FLA 9000 User Manual 28 9607 65 AB 97 A Default sample detection methods Appendix A Default sample detection methods 473 nm laser Reagent name Excitation wavelength Emission light wavelength Filter Alexa Fluor 488 495 519 LPB AttoPhos 482 560 LPB Cy 2 489 506 LPB DBR1 BPB1 DY 485XL 485 560 LPB ECL Plus 420 460 LPB EGFP 489 508 LPB FAM 490 520 LPB FITC 494 520 LPB DBR1 Flamingo 512 535 LPB Pro Q E
57. s related to sample reading can be viewed and changed Not all options are relevant in all scanning modes Se SF E Scan Settings Image Fie Settings Scan Settings Image Fle Settings Comection Mode De Format sito Gel Image Fie 9e C yi C Gei Image File gel JFF Image Fie 3f Launch Apication El Setting Description Correction Mode Auto Use specific image shading correction data that were adjusted in accor dance with each laser Manual The correction method must be selected individually in the Reader Settings window Note The correction mode settings list is registered by a serviceman Please contact the dealer where you purchased Typhoon FLA 9000 or contact GE Healthcare File Format Determines the file format used to save the reading data Gel Image File gel The standard file format is a gel file which contains square root encoded pixel data Gel Image File gel Tiff Image File tif In combination with a gel file a read image can also be saved in TIFF file format For TIFF files image data type is always set to Linear format Launch Applica Contains the path to the application used to analyze the data tion Select the analysis application by clicking the Select button Then browse to the software executable and click OK User account specific settings Changes to the settings in the Preferences dialog affect only the current Windows user acco
58. sample on the Fluor stage Place the sample on the Fluor stage with the chemiluminescent side face down 70 Typhoon FLA 9000 User Manual 28 9607 65 AB 9 Operation 9 6 Placing the object to be scanned on the stage DOEI TEN PLA FLUOR ST ACE LIIG Note Tape the corners of membrane samples to the stage This reduces the effect of physical vibrations Placing the storage phosphor screen on the phosphor stage CAUTION Always wear cotton gloves when handling storage phosphor screens Step Action 1 Turn down the lighting in the room 2 Place the cassette with the exposed storage phosphor screen next to the phosphor stage 3 Pick up the storage phosphor screen and move it to the phosphor stage 1 Press one end of the suction rod against the storage phosphor screen 2 Cover the other end of the suction rod with a finger 3 Liftup the storage phosphor screen by the suction rod and place the storage phosphor screen on the phosphor stage 4 Release the storage phosphor screen by releasing your finger from the suction rod Typhoon FLA 9000 User Manual 28 9607 65 AB 71 9 Operation 9 6 Placing the object to be scanned on the stage Step Action 4 Place the storage phosphor screen on the back of the phosphor stage with the white or blue reading surface of the storage phosphor screen facing up 9 7 Placing the stage in Typhoon FLA 9000 Placing the Fluor or Multi stage in the Typhoon FLA
59. sssssssesesesesennsteseseeseeeesees 76 9 12 Viewing the image file in the analysis application ssesssssssssssssesnsanesssseonveansescxassssatsasinssnvstsssssssnnsgnaasnobonsstnaneoniooneeeh 76 9 13 Turning off Typhoon FLA 9000 2 oss scecasseaiexsstetvensereeenl cassetenssesierstereand le oasdona lene aoe reat 76 Reading conditions display parameters and other settings cccsscecscsecsssssceseesesscsesesssecsesesecsees 77 10 1 Reading conditions CW DEI os disecsce tiez exstecs esecdieeiaesestcesaien daaledieasstaeriebeis en ieneiase an ented 77 10 2 Display parameters explained ansans iai N nena 83 GS RoR lta aec ail see ee ee eT eee ne ee ere aCe ee tee Ten eee SASSA tN AEAT ERATAN E SENESE SETE SEET ear 85 About storage phosphor Screens secre cee eset perce eo tacss emai 86 TET Sa GAN MG a cect ececeee cect E og etre etre aan ee eae 86 11 2 Compatible storage phosphor SCRE SIS axasesovase ence cevscscsnescavsestnenssssasaesoeecsvncnennesezenunncses aes desrneicaeeibnaeneseneacnoieaie 86 11 3 Handing preca tONS mieeeepnmeereenree me eae teor mera Me nererne tiene rePninesrereetaree errenirenteennpy nna a anO RaR RN 86 11 4 Preparing the storage phosphor screen g5sassscs dacsscdciescnivecsyscucascnsdepeoneencceocsnnsanaresesybeciesossouneodeardestneiaaiaedepeseanndessiens 88 11 5 Exposing PE STORAGE phosphor SCHON ace asssssscccssss szedssazasszndsssssssacectansssnceenssncssscecsssatsscanssbancecncssssssiecasssaaedistasasnastd 89 MIGUEL
60. stalling and replacing filters Remove the filter module from the scanner Step Action 1 Click the Filter Module button in the main window A status message appears Wait until the message is closed before proceeding to the next step 2 Press the button on the filter change door 3 Open the filter change door by pulling the knob Typhoon FLA 9000 User Manual 28 9607 65 AB 51 8 Filter module settings 8 4 Installing and replacing filters Step Action 4 Grip the metal tab and pull the filter module straight out Remove and replace filters in the filter module Step Action 1 Pull the green locking lever to the left LPRiitter 52 Typhoon FLA 9000 User Manual 28 9607 65 AB 8 Filter module settings 8 4 Installing and replacing filters Step Action Ps Tilt the filter backwards and pull it out from under the metal lip BPB sie film Ua eth Iti ilter IP 530DF20 ile zaanga aa Sr No gee E JETIM i FUJIFIL Fes PU TER TRAY pan 3 Place the new filter under the rear metal lip and lower the front edge of the filter 4 Push the green locking lever to the right Make sure all filters are locked BPG fritter oen R 570DF20 Typhoon FLA 9000 User Manual 28 9607 65 AB 53 8 Filter module settings 8 4 Installing and replacing filters Place the filter module in the scanner Step Action 1 Open the filter change door O 7 2 Insert the
61. store stages inside Typhoon FLA 9000 92 Typhoon FLA 9000 User Manual 28 9607 65 AB 13 Troubleshooting 13 Troubleshooting This chapter describes various problems that can foreseeably occur with the Typhoon FLA 9000 Control Software Suggestions of possible countermeasures are given Do the following if an error occurs Step Action 1 Take note of the error code and error message on the monitor 2 Turn off the power to Typhoon FLA 9000 and the computer then turn them on again after about ten seconds 3 Try to perform the action again If the error persists contact your GE Healthcare repre sentative General errors and warnings Error message Meaning and countermeasure Failed to open User Manual Please note that a PDF The software required to read the online reader e g Adobe Reader is needed PDF documentation is missing Failed to open End User License Agreement Please Countermeasure Install software for note that a PDF reader e g Adobe Reader is needed Viewing PDF documents Failed to open Getting Started Please note that a PDF reader e g Adobe Reader is needed The disk capacity is insufficient The available disk space is insufficient Countermeasure Free up disk space on the computer Countermeasure Store the data on a dif ferent disk No disk space See above Errors and warnings in the main window Error message Meaning and countermeasure Cannot det
62. tant not to proceed until all stated conditions are met and clearly understood Typhoon FLA 9000 User Manual 28 9607 65 AB 5 1 Introduction 1 1 Important user information Cautions CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood Notices or other equipment NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product Notes and tips Note A Note is used to indicate information that is important for trouble free and optimal use of the product TIP A tip contains useful information that can improve or optimize your procedures Typographical conventions Software items are identified in the text by bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text e g Power switch 1 2 Regulatory information This section lists the directives and standards that are fulfilled by the Typhoon FLA 9000 system Manufacturing information Requirements Content Name and address of manufacturer GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden Name and ID of notified body INTERTEK SEMKO AB NB 0413 Place and date of declaration Uppsala Sweden Sept 2009
63. top of Typhoon FLA 9000 62 Typhoon FLA 9000 User Manual 28 9607 65 AB 9 Operation 9 2 Preparations before starting Typhoon FLA 9000 e There is no stage in the stage rack 9 3 Starting Typhoon FLA 9000 and Typhoon FLA 9000 Control Software Start Typhoon FLA 9000 CAUTION Do not insert a storage phosphor screen in Typhoon FLA 9000 before turning on the machine If a storage phosphor screen is detected during the self diagnosis of the Typhoon FLA 9000 the sensitivity of the storage phosphor screen may deteriorate and reduce the quality of the scanned data Step Action 1 Push the power switch on the right side of the instrument to the I position 2 When the instrument is turned on the On Off conditions of the indicator lamps change as shown below Immediately after turning on power POWER BUSY ERROR y Time up to 30 seconds During self check or startup adjustment 4 Time up to 10 minutes POWER BUSY ERROR Ready for operation POWER BUSY ERROR Typhoon FLA 9000 User Manual 28 9607 65 AB 63 9 Operation 9 3 Starting Typhoon FLA 9000 and Typhoon FLA 9000 Control Software Start Typhoon FLA 9000 Control Software Step Action 1 Make sure that Typhoon FLA 9000 has completed the warm up after which only the power lamp on the upper left panel on the
64. tware 5 2 Install Typhoon FLA 9000 Control Software under Windows XP Step Action 12 Open the USB Control folder Lacate Lile ork jn L USB Zonirol 13 Select the DevMng inf file and click the Open button 14 Click the OK button in the Install from disk dialog 15 Click the Next button in the Add hardware wizard 16 Click the Next button again 17 Click the Continue Anyway button in the Hardware Installation dialog Hardvore nstallotion The voltare pa ae inz aling lor thir haidware FIMA AS 1 S3 Tevi r M rieynent Drives hat rot passac v indowe Lego tasting o veriht t comeatibiiy wth Wirdows tI elme ww this testira is nacranl I Continuing your matallation of this sottware may impair ar destahilize the ranect aperation af gna system either immediately of in the tuturc Microzolt stionaly recommend that you stop thit installation now and contact the hardware vendor for software that has passed Windows Logo testing Centnuea veav STOP Irstalstix 18 Click the Finish button to complete the driver installation 36 Typhoon FLA 9000 User Manual 28 9607 65 AB 5 Installing Typhoon FLA 9000 Control Software 5 2 Install Typhoon FLA 9000 Control Software under Windows XP Install the USB function driver Windows XP Step Action 1 Connect the computer and the Typhoon FLA 9000 with a USB cable and turn ON the power switch of Typhoon FLA 9000 The scanner is automatically d
65. unit when not in use Store the storage phosphor screens in a horizontal position protected from moisture Typhoon FLA 9000 User Manual 28 9607 65 AB 87 11 About storage phosphor screens 11 4 Preparing the storage phosphor screen 11 4 Preparing the storage phosphor screen Procure necessary tools and items Prepare by acquiring the following items e Storage phosphor screen cassette e Radio isotope labeled samples e Gloves e Plastic film e Soft lint free tissues e Ethanol Clean the storage phosphor screen and the cassette Clean the surface of the storage phosphor screen and the inside of cassette with a soft tissue to remove dust and stains Erase the storage phosphor screen The FLA Image Eraser can erase the storage phosphor screen in around 15 minutes if it is not overly exposed For details refer to the FLA Image Eraser User Manual Dim the lighting e Dim the ambient light to 20 lux or less before opening or moving the storage phosphor screen without a cover Set the exposure time Set the exposure time so that the exposure finishes right before the reading starts Note The exposure time of the storage phosphor screen is approximately one twentieth of the time required for X ray film Take this into consideration when setting the initial exposure time TIP For increased image quality minimize the time between exposing the storage phosphor screen and reading it with the Typhoon FLA 9000 88 T
66. unt Changes made using a different user account do not affect the current user account Typhoon FLA 9000 User Manual 28 9607 65 AB 85 11 About storage phosphor screens 11 About storage phosphor screens 11 1 Introduction The Storage phosphor screen is a radiation energy memory type two dimensional sensor which has an image recording layer consisting of polyester base material densely coated with accelerated phosphorescent fluorescent material of fine crystals e Exposure A Storage phosphor screen accumulates and stores radiation energy while it is exposed It is exposed in close contact with an RI sample in a cassette like X ray film e Scanning The recording surface of an exposed Storage phosphor screen is scanned with a laser beam inside Typhoon FLA 9000 and emits fluorescent light according to the exposure level A photo multiplier tube PMT detects the fluorescent light and converts it into electric signals A radiation image recorded on the Storage phosphor screen during exposure is read as digital image information at the maximum resolution of 25 um per pixel 40 pixels mm and recorded in the analyzer unit e Erasure You may reuse a general purpose Storage phosphor screen by erasing an after image 11 2 Compatible storage phosphor screens Storage phosphor screens with magnetic layers for sticking on the phosphor stage are usable 11 3 Handling precautions Check the exposure environment NOTICE gt Avoid e
67. use undesired operation This Class A digital apparatus meets all requirements of the Canadian Interference Causing Equipment Regulations Cet appareil numerique de la classe A respecte toutes les exigences du Reglement sur le materiel bouilleur du Canada Labels on left side of Typhoon FLA 9000 Typhoon FLA 9000 User Manual 28 9607 65 AB 19 2 Safety instructions 2 8 Labels on Typhoon FLA 9000 Labels inside the Typhoon FLA 9000 Interlock parts Never insert any foreign object into the interlock parts to avoid potential laser exposure and injury 2 9 Emergency procedures This section describes how to do an emergency shutdown of the Typhoon FLA 9000 instrument The section also describes the results of a power failure Emergency shutdown Step Action 1 Click the Stop button in the Typhoon FLA 9000 Control Software 2 Turn off the Typhoon FLA 9000 by pressing the power switch on the right side of the in strument body 3 Disconnect the power cord from the power outlet Typhoon FLA 9000 User Manual 28 9607 65 AB 2 Safety instructions 2 9 Emergency procedures Power failure The results of a power failure depends on the unit or units affected Unit affected by power failure Results Typhoon FLA 9000 instrument e The reading is interrupted immediately The instrument is in an undefined state e The data collected up to the time of the power failure is a
68. ut is still available for future use Duplicate Define a new scanning area with the same height and width as the original scanning area Renumber Assign a new number to the scanning area Note Changing the number of one scanning area renumbers all other scanning areas as well Remove Delete the scanning area Confirm the deletion by clicking Yes in the dialog box that opens 80 Typhoon FLA 9000 User Manual 28 9607 65 AB 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Menu choice Description Setting Opens the dialog box Scan Area Settings where the size and position of the scanning area can be finely tuned M Typhoon FLA 9000 User Manual 28 9607 65 AB 81 10 Reading conditions display parameters and other settings 10 1 Reading conditions explained Menu choice Description Function Grid Check to set the size by the positions dictated by the grid on the stage The maximum and minimum values are displayed above the list boxes e Set the position of the lower left corner by changing the list box values in the row Lower Left e Set the position of the upper right coner by changing the list box values in the row Upper Right Note This option is available for the Fluor stage only Millimeter Check to set the size in millimeters The maximum and minimum values are displayed above the list box
69. vailable in the file created when starting the scan Computer running the Typhoon FLA e The computer shuts down immediately 9000 Control Software e The run continues but no data is saved 2 10 Recycling and disposal General instructions for disposal When taking the Typhoon FLA 9000 out of service the different materials must be separated and recycled according to national and local environmental regulations Specific instructions for disposal Measure the superficial radio isotope pollution of the instrument body and storage phosphor screen as mentioned in the radiation hazard prevention section of the User Manual If the pollution level exceeds the limit dispose of the instrument body as radioactive waste Otherwise dispose of the materials according to applicable laws and regulations for disposal of industrial waste Typhoon FLA 9000 User Manual 28 9607 65 AB 21 3 System configuration 3 System configuration 3 1 Parts and functions The parts and their functions are explained separately in this chapter 22 Typhoon FLA 9000 User Manual 28 9607 65 AB 3 System configuration 3 1 Parts and functions Part Function 1 Indicator lamps 2 Lid 3 Handle 4 Instrument cover 5 Power cord connector 6 Power switch 7 Filter holder 8 Filter door 9 USB connector 3 2 Instrument body Power switch The power switch is located on the right side of the Typhoon FLA 90
70. with various fluorescent dyes at high sensitivity and high resolution Yielding very high resolution images equivalent to X ray film Features very high sensitivity wide dynamic range high linearity and high resolution Reusable storage phosphor screens High resolution high sharpness and linearity greatly exceeding film systems Typhoon FLA 9000 User Manual 28 9607 65 AB 1 Introduction 1 4 Typhoon FLA 9000 Control Software 1 4 Typhoon FLA 9000 Control Software System requirements Parameter Minimum requirement Operating system Windows XP Professional SP2 or later or Windows Vista Business SP 1 or later Internal memory 1GB Processor Intel Core 2 Duo processor Hard drive 80 GB Monitor resolution 1280 x 1024 pixels Other requirements One USB 2 0 port DVD ROM drive Overview of the main window The Typhoon FLA 9000 Control Software is used to control use and supervise the Typhoon FLA 9000 8 Typhoon FLA 9000 84 Typhoon FLA 9000 imagination at work CONTROL SOFTWARE 2009 GENERAL ELECTRIC COMPANY ALL RIGHTS RESERVED Technical Support httov www oelifesciences com contact System Settings Function iL Phosphorimaging button click to read a storage phosphor screen 10 Typhoon FLA 9000 User Manual 28 9607 65 AB 1 Introduction 1 4 Typhoon FLA 9000 Control Software Part Function
71. xposing storage phosphor screens in places where the environmental radiation may be increased for example rooms with concrete walls or in basements Do not stack cassettes during exposure 86 Typhoon FLA 9000 User Manual 28 9607 65 AB 11 About storage phosphor screens 11 3 Handling precautions Wear gloves CAUTION Always wear cotton gloves when handling storage phosphor screens Use a suction rod when taking the storage phosphor screen out of the cassette Prying the storage phosphor out may result in the edge peeling off making the storage phosphor screen unusable Handle the storage phosphor screen carefully e Do not scratch or bend the storage phosphor screen e Keep the storage phosphor screen free from dust Protect the storage phosphor screen from water and volatile solvents e Dry the sample thoroughly before exposing it e Wrap wet samples in plastic film and make sure that no liquid exits Use double layers if the sample contains volatile solvents e f water enters the Storage phosphor screen the sensitivity of the storage phosphor screen is reduced e Volatile solvents may deform the protective film on the storage phosphor screen Protect exposed storage phosphor screens from light Protect exposed storage phosphor screens from light until the reading is finished Store the storage phosphor screen correctly Remove the storage phosphor screen from the Typhoon FLA 9000 main
72. yphoon FLA 9000 User Manual 28 9607 65 AB 11 About storage phosphor screens 11 5 Exposing the storage phosphor screen 11 5 Exposing the storage phosphor screen Step Action il Erase the storage phosphor screen completely 2 Wrap the radio isotope sample with plastic film Make sure not to wrinkle the wrapping film Note When using a tritium storage phosphor screen to detect tritium place the sample directly on the storage phosphor screen A tritium storage phosphor screen can be used only once 3 Open the top cover of the cassette Unlock the cassette by sliding the buttons on both sides up 4 Note Keep the radio imaging sample away from the edge of the storage phos phor screen Otherwise the recorded data may become corrupted Typhoon FLA 9000 User Manual 28 9607 65 AB 89 11 About storage phosphor screens 11 5 Exposing the storage phosphor screen 90 Step Action 5 Place the storage phosphor screen in the cassette with the exposure surface of the storage phosphor screen facing the sample Make sure that the notch of the storage phosphor screen is in the front left corner of the cassette as shown below 6 Close the cover of the cassette Press on the cover until it clicks into place Typhoon FLA 9000 User Manual 28 9607 65 AB 12 Maintenance 12 Maintenance Cleaning the outside of Typhoon FLA 9000 Clean the outside with a moist soft cloth and a mild detergent Wipe afterwards with a dry soft cloth

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