Series 200 Diode Array Detector II
Contents
1. TINK POWER o nr kK SET UP 0 0 OFF VIS SERIAL HOST O pini a o ONG CAUTION DOUBLE POLE NEUTRAL FUSING CONTROL ANALOGOUT SPECTRAL DATATO PC GPIB 0 0 0220 of Jo OEDO FUSES 6 3A T 250V Pere AUXF AUXE INST D INS INST B INSTA oo opo 0 0 oo Fuse Holder Power Entry Module System Fuses 6 3A 250 Volt Type T P N 0999 1662 1 of 2 Figure 2 1 Rear panel of the Detector with the system fuses shown Series 200 Diode Array Detector Users Manual 2 5 Detector Power Cord and Fuses The Diode Array Detector power entry module and system fuses are located at the right of the detector s back panel see Figure 2 1 Installing Replacing the Fuses The unit is shipped with two fuses see Figure 2 1 installed To replace a fuse proceed as follows 1 Make sure the unit is disconnected from line power 2 Refer to Figure 2 1 and remove the fuse holder from power entry module at the back of the detector 3 Lift the fuse board lock tab and remove the fuse board from the fuse holder 4 Install or remove and then install fuse s in the fuse holder Use only 6 3 amp T type fuses P N 0999 1662 5 Reconnect the detector to line power The AC Line Cord Ground is necessary for safe operation of the Series 200 Diode A Warning Do not disconnect2. 6 Instrument Performance Verification IPV 6 Routine Inspection and Suitability Test 6 Hazardous Chemicals 7 Definitions in Warning for Hazardous Chemicals 7 Temperature Humidity and Environment 8 Storage Conditions 8 1 Introduction Important 1 1 About this Manual 1 1 Conventions 1 2 Chapter Content 1 2 About the Detector 1 3 Controls Indicators and Connections
3. 2 3 Unpacking Your Detector 2 3 Electrical Requirements and Settings 2 4 Space Requirements 2 3 Detector Power Cord and Fuses 2 5 The AC Line Cord 2 5 Tubing Connections 2 6 Powering up the Director 2 6 3 Turbochrom Connections and Methods About this Chapter 3 1 Hardware Software Requirements 3 1 Communication Connections 3 2 Setting up an LC Method 3 3 Setting the Autosampler Parameters
4. 3 4 Pump Configuration Information 222200 3 6 Creating 200B 250 or 200Q 410 Pump Programs 3 7 LC Detector Parameters 3 9 Setting Instrument Timed Events 3 10 Saving and Setting up the LC Method 3 11 4 Maintenance Lamps 4 1 Ultraviolet Source 4 2 Ultraviolet Source Replacement 4 3 Tungsten Lamp Replacement 4 4 Illustrations Safety and Regulatory Information Location of warning labels 4 1 Introduction 1 1 Front panel of the Series 200 Diode Array Detector 1 3 1 2 Rear panel of the Series 200 Diode Array Detector 1 5 2 Installation 2
5. 1 4 Warnings and Precautions 1 6 Warnings at the Back of the Detector 222000 1 6 Standard Detector Warnings 1 7 COITOSION 1 8 Air Bubbles 1 8 Pressure Buildup 1 8 Specifications 1 9 2 Installation About this Chapter 2 1 Preparing Your Laboratory 2 1 Required Air Quality 2 2 Sufficient Electrical Power 2 2 Solvents Mobile Phase 2 2 Space Requirements
6. LPT 1 to PRINTER Figure 3 1 Workstation Connections 1 HPLC System Series 200 Diode Array Detector User s Manual 3 3 WORKSTATION CONFIGURATION 2 OR MORE DETECTORS PLC SYSTEM 1 55 0214 HPLOSYSTEM2 ___ 4 AUTOSAMPLER _Network Hub __ I COMPUTER LPT 1 to PRINTER Standard Network Cable Figure 3 2 Workstaion connections for Turbochrom Multiple Systems 3 4 Turbochrom Connections and Methods CLIENT SERVER CONFIGURATION AUTOSAMPLER SERIES 200 DETECTOR 55 0214 TO NETWORK PLUG ON WALL SERIES 200 PUMP N2 93 0343 0941 0020 COMPUTER CPU Server for DAD acq LPT 1 to PRINTER Stand To network access comection Figure 3 3 Client Server Configuration Setting up an LC Method In the following paragraphs the autosampler pump and diode array detector parameters are selected for the collection of chromatographic and spectral data To use specific values for the parameters we set up the conditions for the analysis of the universal test mix P N 0089 0893 For configuration information and procedures for building a Note method refer to Chapters 3 and 5 respectively of the T
7. In the list select a tolerance percentage to control when the system Peltier Tray becomes ready The Peltier Tray will be ready to inject only if the temperature is within the range of the temperatures that you set plus or minus the value in the tolerance section Series 200 Diode Array Detector User s Manual 3 7 Pump Configuration Information The Pump Config tab of the Instrument Control dialog box lets you enter labels for solvents and set other pump configuration parameters such as the Ready Time and the pressure units The solvent labels you create appear in the Method Summary window and on the Pump Program tab Setting Pump Configuration Parameters 1 Select the Pump Config tab of the Instrument Control dialog box Instrument Control x Autosampler Pump Config Pump Program Detectors Instrument Timed Events r Pump Operation Ready time min 60 Pressure units PSI g Standby time min fi 5 Max pressure 6200 Standby flow mL min 0 10 Min pressure 0 Solvent Names Solvent amp A Methano Solvent B E water Solvent C essa Solvent D p Cancel Apply Help Time in Ready state after which pump shuts down 15 to 999 2 Inthe Ready Time text box enter the number of minutes that the pump remains in a READY state after which you want the pump to shut down A Ready Time of 999 means that the pump will continue in the READY state indefinitely 3 Inthe Standby Time text box ente
8. e Bandwidth BW A and BW B The bandwidth or total wavelength range around the nominal selected wavelength for Channels A and B e Spectral Acquisition parameters which can be Off and Time If Time is selected you will select the rate at which spectra will be selected To set Series 200 diode array detector parameters 1 Select the Detectors tab of the Instrument Control dialog box Chromatogram Acquisition aS Lae Channel Channel B Wavelength nm 254 280 Bandwidth nm 20 20 Reference wavelength nm 300 200 Spectral Acquisition Program time 5 00 Detector time 5 00 Mode l Time v Spectra s l 1136363 Chromatogram Acquisition Entries 2 Enter 254 nm for the Channel A Wavelength and 280 nm for the Channel B wavelength 3 Enter Bandwidths of 20 nm for both channels 4 Enter 300 nm for both Channel A and Channel B Reference Wavelengths Spectral Acquisition Entries 5 Select the Time Mode 6 Select value 1 136363 in the Spectra s box Series 200 Diode Array Detector User s Manual 3 11 Setting Instrument Timed Events Use the Instrument Timed Events tab of the Instrument Control dialog box to select one or more timed events from a predefined list and enter the time at which you want the event to take place The events available depend on which instrument you have selected For a 900 Series Interface you can choose events to set relays 1 through 7 on
9. Locate the Log On As box and click on the circle next to This Account Click on the box with the 3 periods and choose the Turbochrom process account e usually called tcprocess Enter and confirm the password for this account This password can normally be obtained from the Turbochrom system manager Click on the OK button Next click on the Stop button to stop the service and then click on Start to restart the service This will restart the service with the correct account Start Turbochrom the spectra button should not be grayed out in the bottom right corner of the screen if Turboscan was installed Set up a method in which data is collected for 1 minute then run the method At the end of the run the Status box should have Spectra on the ACQ line At the end of the pump equilibration approximately 30 seconds after the start of the method the status should go to ready Confirm the existence of an spc file with the same name and at the same location as the raw data file essentials To place an order for supplies and many replacement parts request a free e ssentials catalog or ask for information visit our website at www perkinelmer com The most up to date information on part numbers product brochures systems modules spare parts and application notes are in the PerkinElmer website Telephone e US only Call toll free 1 800 762 4000 8 30 a m to 7 p m EST Your order will be shipped usually
10. These tests consist of measuring the most important Diode Array Detector characteristics such as noise drift and wavelength accuracy Certification is available for regulatory compliance Contact your local PerkinElmer Sales and Service office Routine Inspection and Suitability Test Before any sample analysis with the Series 200 Diode Array Detector in the LC system a system suitability test which closely resembles the intended assay should be performed to ensure that the LC system is operating within established criteria e g peak resolution peak asymmetry precision retention time column plate count pressure limits signal noise ratio etc While the Universal Test Mix UTM Part No 0089 0893 can be used for a system check we recommend that you develop a separate system suitability test and acceptance criteria for each of your assays 8 Safety and Regulatory Information Hazardous Chemicals Before using mobile phase solvents you should be thoroughly familiar with all hazards and safe handling practices Observe the manufacturer s recommendations for use storage and disposal These recommendations are normally provided in the material safety data sheets MSDS supplied with the solvents or may become hazardous after completion of an analysis The responsible body e g Lab Manager must take the necessary precautions to ensure that the surrounding workplace and that the instrument operators are not exposed to
11. Turbochrom Connections and Methods before powering up the detector Turbochrom Connections and Methods About this Chapter In this Chapter we discuss the hardware and software requirements when using the Series 200 DAD detector with Turbochrom We then show the Turbochrom electrical communication connections and describe the procedure for setting up and running a test file For procedures for configuring Turbochrom and building a method refer to Chapters 3 and 5 respectively of the Turbochrom Client Server User s Guide P N S270 2600 B Hardware Software Requirements The following are the software requirements for using the Series 200 Diode Array Detector with Turbochrom e Turbochrom WS CS 6 1 or greater e TurboScan e Spectral Acquisition Software that is included with the detector Instructions for using the software are on installation CD N292 2060 3 2 Turbochrom Connections and Methods Communication Connections WORKSTATION C ONFIGURATION 1 DETECTOR AUTOSA MPLER G n 55 0213 SERIES 200 DETECTOR DHT TO NETWORK PLUGON CPU Network Crossover type cable to be u SERIES 200 PUMP 55 A589 N2 93 0343 0941 00 20 COMPUTER CPU
12. Detector is configured with Double Pole Neutral F usi for universal voltage configuration To avoid electrical shock disconnect the power supply before changing fuses Disconnect the power supply whenever the cover is removed AVERTISSEMENT D branchez le cordon d alimentation avant d ouvrir l appareil La continuit du circuit de mise la terre est essentielle pour le bon fonctionnement de l appareil N utilisez jamais l appareil lorsque le c ble de mise la terre est d branch AVERTISSEMENT Pour viter tout risque d incendie ne remplacez les fusibles que par d autres du m me type et de la m me puissance CAUTION DOUBLE POLE NEUTRAL FUSING CONTROL ANALOG OUT SPECTRAL DATATO PC GPIB oo 0E370 0 ALXF AUXE INST DO So oo FUSES 6 3AT 250V NSTC INST B INST A ai Lol oco oE0 o amp o oo Figure S 1 Detector Location of Warning Labels Found on the Rear Panel of the Series 200 Diode Array 6 Safety and Regulatory Information Electrical Warnings Connect the instrument to an AC line power outlet that has a protective ground connection To ensure satisfactory and safe operation of the instrument it is essential that the protective ground conductor the green yellow lead of the line power cord is connected to true electrical ground Any interruption of the protective ground conductor inside or outside the instrument or disconnection of the protective ground terminal may impair the protection
13. To obtain assistance with the Series 200 Diode Array Detector contact the Company as described below PerkinElmer Analytical Instruments 710 Bridgeport Avenue Shelton Connecticut 06484 USA Tel 800 762 4000 Supplies accessories and replacement parts can be ordered directly from PerkinElmer using the eight digit part numbers provided in the manual To place an order or ask for information call your local PerkinElmer representative 2 Safety and Regulatory Information Conventions used in this Manual In this manual the following graphic symbols and special text formats are used to set apart important safety information Warning A warning indicates an operation that could cause personal injury if precautions are not followed Caution A caution indicates an operation that could cause instrument damage if precautions are not followed Note Notes emphasize significant information in a procedure or description Series 200 Diode Array Detector User s Manual 3 Instrument Symbols and Warnings Symbols There are three types of warning symbols that appear on the instrument This symbol indicates an operation or condition on the instrument that could cause personal injury if precautions are not followed This can be any type of hazard When you see this symbol refer to the safety pages in the manual for further information Consulter les documents d accompagnement This symbol indicates the danger of electri
14. Turbochrom manual for error messages On the Rear Panel Figures 1 2 and 2 1 Power Switch An On Off toggle switch located on the power entry module at the right of the rear panel Toggle the switch On to energize the instrument chassis the cooling fan and the photodiode array assembly Power Cord Connector Receptacle Accepts a standard detachable three prong power cord Power Fuse Holder Includes two 6 3A T type fuses for main power Lamp Adjustment Thumb Wheels For use by Service Representatives only LINK POWER o TS Network Port SERI ALMOST oer co ae wi CAUTION DOUBLE POLE NEUTRAL FUSING CONTROL ANALOG OUT SPECTRAL DATATO PC GPS oo oo e o o AUXF AUXE INST D Fuse Holder Power Entry Module Figure 1 2 Rear panel of the Series 200 Diode Array Detector 1 6 Introduction Warnings and Precautions Warnings at the Back of the Detector The following warnings are placed on the back panel of the detector see Figure S 1 They contain information that you should be familiar with before operating the Diode Array Detector N Warning N Caution DISCONNECT POWER BEFORE SERVICE GROUNDING CIRCUIT CONTINUITY IS VITAL FOR SAFE OPERATION OF EQUIPMENT NEVER OPERATE EQUIPMENT WITH GROUNDING CONDUCTOR DISCONNECTED For protection against fire hazard replace only with the same type and rating of fuse Double Pole Neutral Fusing The detector is configured with Double Pole Neutral Fusing for universal
15. or off Another event RVPRT instructs the interface to read rack and vial information from the attached autosampler at a specified time For information about the instrument timed events for other supported instruments refer to the documentation that came with the instrument In the Graphic Method Editor you can insert instrument timed events directly on the chromatogram at a specified time For more information refer to Chapter10 of the Turbochrom Workstation User s Guide Editing a Method and Results Graphically Saving and Setting up the LC Method See chapter 5 Building a Method of the Turbochrom Client Server Users Guide P N S270 2600 B It provides an overview of the Turbochrom Method Editor and of creating and modifying a method A method consists of instrument processing and calibration parameters which are described in chapters 6 7 and 8 respectively of the user s guide 3 12 Turbochrom Connections and Methods Lamps Maintenance The Series 200 Diode Array Detector uses a deuterium lamp and or a tungsten halogen lamp as light sources See Figure 4 1 Warning The deuterium lamp operates at high voltage Remove A power to the instrument to avoid electrical shock Warning The ultraviolet light produced by deuterium lamps will cause eye and skin damage Always wear protective glasses and avoid looking directly at the light output when working with deuterium lamps Warning The deuterium lamp opera
16. problems e g unexpected absorbance bands or contamination Dirty or cracked cell windows Detector needs to be realigned Lamp replacement is required Ultraviolet Source Replacement The part number of the deuterium lamp is N292 2046 Replacement lamps are obtained by calling your normal parts maintenance number Note Tools Required To replace the deuterium lamp see Figure 4 1 you need a 3 32 in hex driver Warnings and Precautions fingerprint on the glass could cause the bulb to explode N Warning Never touch the exposed glass of the deuterium lamp A when the operating temperature is attained Fingerprints will always compromise performance N Warning Always wear UV safety glasses when working near the detector Stop the pump if it is running turn off power to the detector and disconnect the flowcell connections Then disconnect the detector power cord from the rear panel of the detector Remove the flow cell cover plate by loosening the four knurled securing screws Remove the flow cell by loosening the knurled screw at the base of the cell carriage and sliding it out of the instrument Visually check the flow cell and make sure that there are no air bubbles in the cells or cracks in the cell windows that will interfere with test procedures These factors can contribute to low lamp energy Allow enough time for the deuterium lamp and the lamp housing to thoroughly cool Disconnect the electrical p
17. provided by the instrument Do not operate the instrument with any covers or parts removed Do not attempt to make adjustments replacements or repairs to this instrument except as described in the accompanying User Documentation Only a PerkinElmer service representative or similarly trained and authorized person should be permitted to service the instrument Use only fuses with the required current rating and of the specified type for replacement Series 200 Diode Array Detector User s Manual 7 Quality Control Good Laboratory Practices Quality Control The user should develop appropriate quality control procedures for the LC Diode Array Detector and the entire LC system to ensure suitability for its intended use These procedures typically consist of periodic performance verifications and routine inspections and suitability tests Certificate of System Control Each Series 200 Detector is carefully built and tested in a controlled system in accordance with the requirements specified in its applicable Final Assembly and Test Specification Each instrument is certified to meet its functional and performance specification upon release to shipment Instrument Performance Verification IPV To maintain functional performance PerkinElmer recommends a yearly Instrument Performance Verification IPV of the Series 200 Diode Array Detector by a PerkinElmer Service Engineer to ensure its operation within published specifications
18. the height at which the sampling needle extracts the sample It is set as a percentage of the vial height measured from the bottom of the vial In the Inject Delay Time text box enter the number of minutes that you want between the pump s run start signal and when the sample injection occurs If you are using a Series 200 Autosampler refer to Creating Derivatization and Dilution Programs In the Flush Volume text box enter the amount of flush solution that you want pumped through the sampling system for each flush cycle From the Flush Speed list select the speed at which you want the pump to flush the system In the Flush Cycles text box enter the number of flush cycles that you want to have between injections when the method changes in a sequence This value also specifies the number of flushes the autosampler performs when you choose Flush Autosampler in the Hands On dialog box In the Pre Injection Cycles text box enter the number of flush cycles that you want before each sample injection or before each vial to vial transfer in sample processing modes In the Post Injection Cycles text box enter the number of flush cycles that you want after each sample injection or before each vial to vial transfer in sample processing modes In the Post Method Cycles text box enter the number of flush cycles that you want after each method is completed in a sequence Under Peltier Tray Control set the temperature of the tray
19. voltage configuration To avoid electrical shock disconnect the power supply before changing fuses Disconnect the power supply whenever the cover is removed Series 200 Diode Array Detector User s Manual 1 7 Standard Detector Warnings Read and understand the following warnings and precautions Solvents Warning Give careful attention to the hazards associated with the solvents you are using Refer to the safety data sheets provided by the manufacturer For example Material Safety Data Sheets MSDS in the USA e Wear appropriate eye protection at all times when handling chemicals Use safety glasses with side shields goggles or full face shields according to the types of chemicals you will be handling e Wear suitable protective clothing including gloves that are specifically resistant to the chemicals being handled e Always use clean solvents Solvents that have been distilled in glass HPLC Grade are highly recommended e Filter the solvents through a 0 5 micron medium as an additional precaution e Carefully use and store flammable solvents which may form hazardous by products when the instrument is shut down by following the recommended shutdown procedure e Check compatibility of solvent s with the type of column s being used e Know the relative polarity and miscibility of the solvents being used Buffers Exercise care when using buffers in conjunction with organic solvents NEVER LEAVE BUFFERS IN TH
20. within 24 hours e Worldwide Call your local PerkinElmer sales or service office PerkinElmer precisely PerkinElmer 710 Bridgeport Avenue Shelton CT 06484 4794 U S A Internet http www perkinelmer com email info perkinelmer com PerkinElmer is a registered trademark of PerkinElmer Inc Part Number 0993 6475 B
21. 1 Rear panel of the Detector with the system fuses shown 2 4 2 2 AC outlet configurations 2 5 2 3 Series 200 Diode Array Detector in a typical LC system 2 6 3 Turbochrom Connections and Methods 3 1 Network Client Server connections 3 2 3 2 Workstation connections for Turbochrom 1 HPLC System 3 3 3 3 Workstation 4 Maintenance 4 1 The Detector lamps 4 2 4 2 Removing the mercury timer switch for disposal 4 4 4 3 Aligning the beam at the center of the cell focus mirror 4 6 Appendix A A 1 Connections at the rear of the Diode Array Detector A 2 Safety and Regulatory Information Electromagnetic Compatibility EMC United States This product is classified as a digital device used exclusively as industrial commercial or medical test equipment It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section 15 103 c Addresses
22. Conlon published by PerkinElmer 1980 Part No 0993 9656 1 2 Introduction Conventions The following conventions are used in this manual see the Safety and Regulatory Information preceding this chapter Warning A warning indicates an operation that could cause personal injury if precautions are not followed Caution A caution indicates an operation that could cause instrument damage if precautions are not followed Note Notes emphasize significant information in a procedure or description Chapter Content This chapter describes the detector its controls and detector specifications see Table 1 2 The second chapter describes installation of the detector in your LC system electrical requirements and plumbing connections The third chapter is comprised of drawings Figures 3 1 and 3 2 showing communications connections for Turbochrom and the procedure for setting up an LC method for running a test file Chapter 4 Maintenance includes lamp replacement procedures e replacing the deuterium UV lamp e replacing the tungsten VIS source including an alignment procedure Series 200 Diode Array Detector User s Manual 1 3 About the Detector The Series 200 Diode Array HPLC high performance liquid chromatography detector is a high performance photodiode array detector It includes a dispersive photodiode array spectrometer system that continuously collects full scan spectra of the analytes in a flowing stre
23. Diode Array Detector has an internal link The Connections at the rear are as follows see Figure A 1 SERIAL HOST Serial or Terminal server to Turbochrom SPECTRAL DATA TO PC To LPT port of local client This LPT port cannot be used for a printer make sure none are configured for it INST A Port A to the pump INST B Port B to Control Series 200 DAD INST C Port C to the autosampler Network Port A 2 Configuring Series 200 DAD for Acquiring Spectra LINK POWER Network Port SERIALHOST ss So eerie pags CAUTION DOUBLE POLE NEUTRAL FUSING CONTROL ANSLOGOUT SPECTRAL DATA TO PC GPB o0 oo 00 0H AUXF AUXE INSTO DI D oo o0 0 0 oo Fuse Holder Power Entry Module Figure A 1 Connectors at the rear of the Diode Array Detector Table A 1 Proper Port Configuration PORT DEVICE Port INST A Pump Port INST B Series 200 DAD Port INST C Autosampler Port INST D none Network Port See Note Below Turboscan Installation If Turboscan has been purchased install it as described in Chapter 2 of the Turboscan manual part number 0993 8917 Note Also refer to the procedure for installing Turboscan in the Help multimedia files in the Service CD Installation of Turboscan Note The procedure following for adding servers ports and for configuring the Detector are described in detail in Help multimedia files on supplied CD part number N292 2060 They are located
24. E SYSTEM OVERNIGHT Buffers left in the system can form salt crystals that may cause premature pump seal failure interfere with proper check valve operation and plug the connecting tubing or the detector flowcell To remove buffers flush the system first with water then with methanol or isopropanol Change the mobile phase from methanol or isopropanol to water before using buffers 1 8 Introduction Corrosion All parts that contact the mobile phase are made of stainless steel Teflon PEEK or quartz Some of these materials are extremely sensitive to acid chlorides If you have questions about your mobile phase contact a Service Representative Table 1 1 Solvents Which May Corrode the Series 200 Diode Array Detector Aqua Regia 80 HCl 20 HNO3 Chloride salt solutions Chlorinated solvents Halogenated solvents Titanium is more resistant to chloride salt solutions Air Bubbles To prevent air from entering the system and to ensure that pressure fluctuations do not occur observe the following precautions e Ensure that the pump s solvent inlet filter is below the solvent level in the solvent reservoir e Ifthe pump has not been used for an extended period of time remove air bubbles by connecting a priming syringe to the drain valve on the pump opening the drain valve and fast flushing the system to prime the pump After priming the pump close the drain valve completely e If bubbles are observed in the flow cel
25. LIQUID CHROMATOGRAPHY SERIES 200 DIODE ARRAY DETECTOR II User s Manual P PerkinElmer precisely Series 200 Diode Array Detector II User s Manual Release History Part Number Publication Date 0993 6475 September 1999 0993 6475 B December 2001 Any comments about the documentation for this product should be addressed to User Assistance PerkinElmer 710 Bridgeport Avenue Shelton CT 06484 4794 Or emailed to info perkinelmer com Notices The information contained in this document is subject to change without notice Except as specifically set forth in its terms and conditions of sale PerkinElmer makes no warranty of any kind with regard to this document including but not limited to the implied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing performance or use of this material Copyright Information This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior written permission of PerkinElmer Inc Copyright 1999 2004 PerkinElmer Inc Produced in the U S A Trademarks Registered names trademarks etc used in this document even when not specifically marked as such are p
26. alytical 2 mm path 4 uL preparative Dimensions Height 19 7 cm 7 75 in Width 33 7 cm 13 25 in Depth 80 5 cm 22 75 in 20 kg 45 Tb 1 10 Introduction Installation About this Chapter The following paragraphs are included in this chapter to prepare you for installing the Series 200 Diode Array Detector preparing your laboratory unpacking your detector electrical requirements and settings tubing connections powering up the detector Preparing Your Laboratory Before installing your Series 200 Diode Array Detector prepare your laboratory according to the following guidelines 2 2 Installation Required Air Quality To minimize contamination problems in your laboratory provide a relatively dust free environment Make sure that gases or vapors of the following kinds are not present at levels that exceed federal state or local ordinances for continuous human exposure e flammable caustic e explosive e corrosive e toxic Make sure that your laboratory environment includes the following temperature and humidity levels e Ambient temperature between 10 C and 35 C e Constant relative humidity between 20 and 80 without condensation Be careful when working with hazardous solvents or solvents that produce hazardous by products Warning Solvent vapor levels that are high enough to interfere with detector performance should be considered hazardous to someone who is continuously exposed to
27. am The detector consists of the opto electronic assembly and a data system the storage of spectral files is controlled by sending commands from the data system to the spectrometer system Deuterium Lamp ON OFF Switch Tungsten Lamp ON OFF Switch Access Cover Link Status Indicator Lamps Figure 1 1 Front panel of the Series 200 Diode Array Detector The opto electronic assembly consists of e an optical bench with a flow cell e regulated deuterium and tungsten light sources e polychromator spectrograph e 512 element photodiode array e power supplies and circuitry required to operate the detector and transmit data to the data system The data system consists of an on board microprocessor that communicates via an RS 232 connection with a host data system 1 4 Introduction Controls Indicators and Connections The controls indicators and connectors on the front and rear panels of the detector are described in the next two paragraphs On the Front Panel Figure 1 1 UV Switch Press the switch to energize the deuterium lamp When the lamp is energized the switch is lighted Only energize the deuterium lamp when the sample absorbs in the UV range 190 360 nm Note Wait two minutes after turning on the Power switch before energizing the deuterium lamp VIS Switch Press the switch to energize the tungsten halogen lamp When the lamp is energized the switch is lighted Only energize the tung
28. any other connectors Unpacking Your Detector Carefully unpack the Series 200 Diode Array Detector and check for obvious signs of damage that could have occurred during shipment Immediately report any damage or missing items to the shipping carrier and Perkin Elmer A start up kit is supplied with the detector Refer to Table 2 1 and check the parts you received against the parts listed in the table Table 2 1 Start Up Kit for the Series 200 Diode Array Detector Items Included PerkinElmer P N AC Line Cord 0998 8986 Fuse 6 3A 250V IEC Type T 0999 1662 in PEEK Fingertight Nut 0992 0513 bers Mami se 1 Harns Link to Detector 55 A589 Cable Service 9PF to 9PF Dsub 6 55 A588 1 Customers located outside of the U S must order a line cord that is appropriate for their location Vendor GTI Part Number 2 4 Installation Electrical Requirements and Settings The Series 200 Diode Array Detector can operate at 50 or 60 Hz and requires a grounded outlet that provides a nominal 100 120 220 or 230 240 VAC source If the electrical supply voltage in your laboratory produces large AC line voltage fluctuations install a voltage regulator between the AC line voltage outlet and the Series 200 Diode Array Detector Instrument grounding is required and is accomplished by a third wire in the AC line voltage outlet box If your electrical outlet does not contain a ground contact your local electric company
29. c shock if precautions are not followed due to the presence of high voltage Attention Risque de choc lectrique not followed due to the presence of heat in this area of the instrument There may also be hot surfaces that can be easily touched AVERTISSEMENT Surface chaude N This symbol indicates the danger of burns if precautions are Additional graphic symbols used on the instrument are the following Indicates alternating current Indicates the primary protective grounding terminal Indicates the off position of the main power switch Indicates the on position of the main power switch 4 Safety and Regulatory Information Warnings The following safety marking symbols and labels are affixed to the rear panel of the instrument See Figure S 1 DISCONNECT POWER BEFORE SERVICE GROUNDING CIRCUIT CONTINUITY IS VITAL FOR SAFE OPERATION OF EQUIPMENT NEVER OPERATE EQUIPMENT WITH GROUNDING CONDUCTOR DISCONNECTED Warning For protection against fire hazard replace only with same type and rating of fuse Series 200 Diode Array Detector User s Manual 5 Warning Disconnect supply cord before opening Grounding circuit continuity ss vital for safe operation of equipment Never operate equipment with grounding conductor disconnected Warning For protection against fire hazard replace only with same type and rating of fuse SERIALHOST o 100 240 VAC 5060 Hz 195VA Warning The
30. gree This equipment will operate safely in environments that contain nonconductive foreign matter up to Pollution Degree 2 in IEC 1010 1 Storage Conditions The Series 200 Diode Array Detector may be stored under the following conditions e ambient temperature is 20 to 60 C 68 to 140 F e ambient relative humidity is 20 to 80 noncondensing e altitude is in the range 0 to 12 000 m Introduction Important This manual contains important information regarding potential hazards that may arise during the operation of the Series 200 Diode Array Detector It is essential that the Safety and Regulatory mformation preceding this chapter be read and thoroughly understood by all users and potential users of the detector Caution The Diode Array detector should be used according to the A instructions provided in the manual If used otherwise the protection provided for the Diode Array detector might be impaired About this Manual This manual was written for a person who has developed a working knowledge of liquid chromatography and understands e the function of each major component in a liquid chromatography system e how to select a column based on the analysis that will be performed e the principles of solvent selection and sample preparation If you require an introduction to these concepts the following book is an excellent reference Practical Liquid Chromatography An Introduction by R W Yost L S Ettre and R D
31. hazardous levels of toxic substances chemical or biological as defined in the applicable Material Safety Data Sheets MSDS or OSHA ACGIH or COSHH documents Venting for fumes and disposal of waste must be in accordance with all national state and local health and safety regulations and laws Warning Some chemicals used with this instrument may be hazardous Definitions in Warning for Hazardous Chemicals Responsible body Individual or group responsible for the use and maintenance of equipment and for ensuring that operators are adequately trained per IEC 1010 1 Amendment 2 Operator Person operating equipment for its intended purpose per IEC 1010 1 Amendment 2 OSHA Occupational Safety and Health Administration United States ACGIH American Conference of Governmental Industrial Hygienists COSHH Control of Substances Hazardous to Health United Kingdom Series 200 Diode Array Detector User s Manual 9 Temperature Humidity and Environment Caution This instrument is designed for indoor use only Warning This instrument is not designed for operation in an explosive environment Safe Operation This detector will operate safely between 10 and 35 C 50 and 95 F with an ambient relative humidity of between 20 and 80 noncondensing Installation Category This detector is able to withstand transient overvoltage according to Installation Category II as defined in IEC 1010 1 Pollution De
32. he bulb 11 Slide the cap back down over the new lamp and allow the lamp to seek it s own center 12 Rotate the lamp cap until the lamp is vertical and secure the lamp by tightening the setscrew Then check to make sure that the lamp is held securely by the lamp cap 4 6 Maintenance 13 Turn on the detector power switch and the VIS power switch 14 Refer to Figure 4 3 and use a white piece of paper or card business cards are ideal to check if the light image falls on the center of the cell focus mirror If it doesn t you must loosen the setscrew see Step 12 and adjust the bulb s position to achieve proper alignment Then retighten the setscrew 100mm Cell Focus Mirror White Card and Holder Assembly Vi Visible Light Beam Field Stop MM1 2 Figure 4 3 Aligning the beam at the center of the cell focus mirror 15 Turn off the detector power and replace the cell focus mirror cover and the detector cover 16 Replace the flow cell and cover plate Appendix Configuring Series 200 DAD for Acquiring Spectra This appendix includes the procedure for configuring the Series 200 Diode Array detector for both Turbochrom Client Server and Turbochrom Workstation software Installation Requirements Client Server installation requires a Windows NT 4 0 Workstation Workstation installation requires Windows 95 98 Connectors at the Back of the Detector The Series 200
33. he bulb will break if undue force is applied Caution Never touch the bulb with your bare hands Residues from your skin can damage the bulb Replacement procedure The lamp is held by a top cap assembly as well as two leads Proceed as follows to replace the tungsten lamp 1 Stop the pump if it is running and turn off power to the detector 2 Disconnect the flowcell connections 3 Disconnect the detector power cord from the rear panel of the detector 4 Remove the flow cell cover plate by loosening the four knurled securing screws Then remove the flow cell by loosening the knurled screw at the base of the cell carriage and sliding it out of the instrument 5 Remove the top cover of the detector by loosening and then removing the four cabinet screws and sliding the instrument cover forward Remove the cell focus mirror cover by loosening the two 3 32 in hex screws 6 Visually check the flow cell and make sure that there are no air bubbles in the cells or cracks in the cell windows that will interfere with test procedures 7 Using a 0 050 in hex key wrench loosen the setscrew on the side of the lamp cap Slide the cap up and off the post 8 Remove the lamp from the holder 9 Grasp the new bulb in its protective bag and cut off the bottom of the bag thereby exposing the contacts 10 Line up the leads with the receptacle contacts in the holder and push the bulb into place There is no particular polarity needed for t
34. in the Installation directory of the CD Note The network plug can be used to control the detector and acquire spectral data The server can be a local workstation computer or a server located elsewhere on the network Configuration of this port needs to be done by a PerkinElmer service engineer Series 200 Diode Array Detector User s Manual A 3 Adding a Server Port and Instrument Note The procedures in this paragraph steps through 4 below are for Turbochrom Client Server software only NOT for Turbochrom Workstation 1 The name of the New Server should be the same as the name of the local workstation through which the LPT port is connected to the Series 200 DAD To determine the name right click on the Network Neighborhood icon then choose Properties from the menu The name to use for the New Server name will be in the Computer Name field 2 Choose Windows NT as the operating system Then select check only the Analysis Server the Directory Replication Host and LM Host are to be left unchecked Finally select a default printer for the New Server 3 Adda port for the detector Choose the New Server configured above for the acquisition server field 4 Add an instrument for the detector Assign it to any applicable instrument groups Configuration If applicable save the entries made above and configure as follows 1 Configure the instrument to the appropriate port listed see Table A 1 and Figure A 1 The de
35. in the pump program Step 0 is always the pre run equilibration for which you enter Time Flow and Solvent values The table for 200B 250 or 200Q 410 pumps has the following columns e Time duration of each program step e Flow flow rate during each program step e A B C Solvents percentage of each solvent at the end of each program step e D Solvent The percentage of this solvent at the end of each program step This value adjusts automatically so that the sum of A B C and D is 100 e Curve type of gradient curve to use for this program step The types of curve are step 0 linear 1 convex 1 1 to 9 9 and concave 1 1 to 9 9 for increasing solvent percentage The magnitude of the number determines the degree of curvature Series 200 Diode Array Detector User s Manual 3 9 To set pump control parameters 1 2 Select the Pump Program tab of the Instrument Control dialog box The Program Time field displays the sum of the values in the Time column for all the steps that you create The Detector Time field displays the data acquisition time of the analysis Note In Step 0 of the table click in the Time Flow and solvent fields and then enter amounts for the equilibration step Enter the Time Flow rate solvent composition and Curve type for each additional program step The Time field activates the other fields in each row and the curve changes as you enter each set of values Depending on the
36. l degas the solvent and add back pressure device 0990 7126 Pressure Buildup Over time you may notice a gradual increase in the system operating pressure If you observe pressure readings greater than 3 45 MPa 500 psi above the normal operating pressure of your analysis check the following items e If you are injecting clean samples and there is no other apparent cause for the pressure buildup remove disassemble and clean the injector e If you are injecting dirty samples the injector may be clogged or the column packing material may have retained contaminated particulates Remove and clean the injector and replace the column e The column end fitting or column frit may be plugged Replace the end fitting or frit to relieve excess pressure Refer to the instructions supplied with the column e In line filters may be plugged Replace the filter element e With the pump connected directly to the detector input if you still experience excessive pressure the problem could be a plugged flowcell You can flush reverse flush or rebuild the flowcell Series 200 Diode Array Detector Users Manual 1 9 Specifications Table 1 2 Specifications of the Series 200 Diode Array Detector Detector Specification Description Resolution 4 nm observed 1 09 nm diode Short term Noise 1 x 10 AU average peak to peak noise between 190 and 700 nm dry cell 3 Flow Cells 10 mm path 12 uL standard 4 5 mm path 5 uL an
37. on loop fitted to your autosampler Note To enable the Fixed Mode option where the injection volume is equal to the loop volume select a loop size value less than 50 uL 5 To operate in Fixed Mode set the value of the Fixed Mode list to On This option forces the Injection volume to be equal to the loop volume Fixed mode is useful if you are working with small sample sizes or applications that require high precision You can only use Fixed Mode when the Loop Size value is less than 50 uL 6 Inthe Excess Volume text box enter the amount of sample that you want withdrawn from the vial in addition to the injection volume This option prevents evaporation and contamination by isolating the actual plug of sample to be injected as it is transported to the injection loop This option is only available if Fixed Mode is set to Off 7 Inthe Air Cushion text box enter the amount of air that you want to insert between the sample and flush solutions to prevent the two solutions from mixing 3 6 Turbochrom Connections and Methods 10 11 12 13 14 15 16 17 18 19 20 From the Sample Syringe Size list select a syringe volume From the Sample Speed list select the speed at which you want the sample pump to fill the injection loop Slower speeds provide more reproducible injection volumes particularly for viscous samples From the Needle Level select the appropriate percentage This value sets
38. or defeat safety ground on the power cord 1 2 array detector and proper functioning of the electronic circuitry The line cord should have the electrical rating and plug appropriate for your location See Figure 2 2 for the correct cord Select the proper line cord for your location Figure 2 2 Insure that the power switch is off and insert the line cord plug into the AC supply i Old British Standard North America NEMA 5 15 rl 0999 1420 0999 1423 Europe British Standard SE CEE 7 BS 1363 CUKO United Kingdom 0999 1415 0999 1414 Europe Australia Switzerland ETSA S 86 0999 1413 0999 1417 Europe Israel Italy 0999 1424 0999 1422 Denmark 0999 1416 Figure 2 2 AC outlet configurations 2 6 Installation Tubing Connections The flow cell is shipped with 0 007 in ID standard stainless steel tubing installed All connections are made with standard1 16 in fingertight fittings In Figure 2 3 the Series 200 Diode Array Detector is shown plumbed in a typical LC system Bottom View of the Series 200 Autosampler njector Valve ES Waste Series 200 Solvent Diode Array Detector In Line Solvent Filter Optional Figure 2 3 Series 200 Diode Array Detector in a typical LC system Powering up the Detector After completing the installation of the Diode Array Detector carefully read Chapter 3
39. ower leads from the deuterium lamp Loosen and remove the two 6 screws that hold the deuterium lamp to the housing using the 3 32 in hex driver and remove the lamp The deuterium lamp contains a mercury timer switch Remove the timer switch from the lamp assembly as shown in Figure 4 2 and dispose of it properly 4 4 Maintenance Access Cover Deuterium Lamp Mercury Cut wire at places indicated CU Remove switch and dispose of properly Deuterium Lamp removed from mount View behind Access Cover Deuterium Lamp disposal of mercury switch Figure 4 2 Removing the mercury timer switch for disposal 8 Install the new deuterium lamp and then reconnect the electrical power leads 9 Install the flow cell and cover plate Tungsten Lamp Replacement Note The part number of the tungsten lamp is N292 2011 Replacement lamps are obtained by calling your normal parts maintenance number Tools Required To replace the tungsten lamp see Figure 4 1 the following tools are needed e 0 050 inch hex driver e 3 32 inch hex driver e 5 32 inch hex driver Series 200 Diode Array Detector User s Manual 4 5 Warnings and Precautions Warning Never touch the exposed glass of the tungsten lamp A fingerprint on the glass could cause the bulb to explode when the operating temperature is attained Fingerprints will definitely compromise performance Caution The bulb walls are fragile T
40. r the number of minutes that the pump remains in a READY state after which you want the pump to change to the standby flow rate A Ready Time of 999 means that the pump will continue to use the flow rate set in the grid as long as the pump remains READY 4 Inthe Standby Flow text box enter the pump flow rate for the standby mode 5 From the Pressure Units list select either PSI or BAR as the units for inlet pressure the choice depends on the instrument that you are using 6 Inthe Max Pressure text box enter the upper pressure limit above which the pump will shut off 7 Inthe Min Pressure text box enter the lower pressure limit below which the pump will shut off 8 Inthe Solvent Names text boxes enter the labels you want to use 3 8 Turbochrom Connections and Methods Creating 200B 250 or 200Q 410 Pump Programs To create a pump solvent gradient program or pump program use the Pump Program tab of the Instrument Control dialog box This tab contains a table and a gradient curve which let you define the composition of the solvents at each step in the run You can change the table parameters and the curve either by editing the values in the table or by dragging points on the curve with the mouse Programtime 5 00 0 0 i 360 Detector time 5 00 0 1000 00 1000 O0 1000 06 100 0 TT Enable solvent saver TT Shutdown in final conditions Saver equil time min fi 0 You can enter 10 rows or steps
41. rotected by law PerkinElmer Turboscan and Turbochrom are registered trademarks of affiliates of PerkinElmer Inc PerkinElmer is a registered trademark of PerkinElmer Inc Contents Safety and Regulatory Information Electromagnetic Compatibility EMC 1 United States 1 Addresses 1 Conventions used in this Manual 2 Instrument Symbols and Warnings S Symbols 3 Warnings 4 Electrical Warnings 5 Quality Control Good Laboratory Practices 6 Quality Control 6 Certificate of System Control
42. specific pump that you are using Turbochrom completes the solvent percentage either for solvent B or for solvent D so that the total solvent composition is 100 percent Also if you change the value of solvent B or the value of solvent D the value of solvent A adjusts so that the total remains 100 percent For the 250 pump the resolution at which you can enter time and flow values changes across the allowed range You can enter times between 0 and 9 9 minutes to the nearest 0 1 minute but times between 10 and 999 minutes must be to the nearest minute Flow rates between 0 and 0 99 mL min can be to the nearest 0 01 mL min but flows between 1 0 and 10 0 mL min can only be to the nearest 0 1 mL min Turbochrom will beep if your entry causes a change from one resolution range to another Note To reduce the amount of equilibration time after the first run select Enable Solvent Saver and then enter the new amount of equilibration time to use after the first run in the text box To turn the pump off at the end of the run without recycling to initial conditions select Shutdown In Final Conditions Only set this option for a shutdown method which is run as the last cycle in a sequence Note 3 10 Turbochrom Connections and Methods LC Detector Parameters The Detectors tab of the Instrument Control dialog box is used to set detector parameters e Wavelength A nm and B nm The wavelength selected to monitor Channels A and B
43. sten halogen lamp if the sample exhibits absorption in the VIS range 360 700 nm Note If you are using both UV and VIS lamps turn on the VIS lamp AFTER turning on the UV lamp To control the lamps from Turbochrom both lamps must be switched on prior to taking control from Turbochrom Navigator After performing Method Setup you can turn the lamps on or off using Hands On See the Turbochrom Client Server User s Guide P N S270 2600 B Note Cell Carriage Holds the flow cell When inserted it keeps the flow cell in the correct position in the optical path of the detector To remove the cell carriage from the detector remove the access cover see Figure 1 1 and loosen the carriage retaining screw Inlet and Provide connections 0 007 in stainless steel tubing to the outlet of Outlet Lines the HPLC column and the waste container Front Panel Indicator Lamps The following indicator lamps are on the front panel of the detector see Figure 1 1 System Power lamp lights when the Power On Off switch on the back panel is in the ON position UV lamp lights when the deuterium lamp is energized VIS lamp lights when the tungsten halogen lamp is energized Series 200 Diode Array Detector Users Manual 1 5 Link Status Indicator Lamps Link Ready lights when a link is ready Link Power lights when a link is powered on Channel Status lamps Three Channel Ready Lamps Three Active Channel Lamps Three Channel Error Lamps Refer to the
44. tector power must be ON that is the green light on the front panel must be lit Note The detector lamps can be off however the lamps should be turned on before taking control from Turbochrom Navigator If both the UV and VIS lamps are not depressed and the lamps are not on before taking control you will not be able to turn the lamps on or off using Hands On As with the LC235 Diode Array Detector the lamp will then be started automatically at Power ON Note We also recommend that you turn on the Series 200 Diode Array Detector and initialize it for two minutes before taking control with Hands On Taking control sooner could result in Instrument Error 61 2 Using the CD provided with the Diode Array Detector P N N292 2060 install the Spectra Daemon and the Series 200 Diagnostics software If a port other than LPTI is used please refer to the readme file that is included with the software This will enable you to edit the registry to change the port to another LPT port 3 Reboot the computer when and if you are asked to do so A 4 Configuring Series 200 DAD for Acquiring Spectra d For Windows NT workstations only perform steps 4a through 4f For Windows 95 workstations proceed to step 5 a b c After the Spectra Daemon is installed press Start gt Settings gt Control Panel then click on the Services icon Locate the 200 Spectral Acquisition service and highlight it Then click on the Startup button
45. tes at high temperatures in excess of 200 C 390 F The lamp housing is hot when the detector is operating Do not touch the lamp or the housing when the detector is operating Caution The lamp filaments are fragile Do not subject the lamp to vibration or shock 4 2 Maintenance o 0 0 000 0 0 o o o 07 Access Cover Deuterium Lamp inside mount Tungsten Lamp View behind Access Cover Figure 4 1 The Detector lamps Ultraviolet Source The ultraviolet source produces an intense stable light by maintaining an electric arc in a deuterium atmosphere This emitted light is a continuous wavelength spectrum from 190 nm to 400 nm Due to varying operating conditions the useful life of a deuterium lamp can not be precisely determined However when the lamp intensity decreases to 50 of the original output the lamp has reached the end of its useful life The deuterium lamps used are guaranteed for 500 hours of operation If the lamp fails within that time it will be replaced free of charge Ifthe lamp fails within the next 500 hours it will be replaced on a pro rated basis If the deuterium lamp appears to be emitting low energy you should eliminate all other possible causes of low sensitivity before replacing the lamp Series 200 Diode Array Detector User s Manual 4 3 Low sensitivity may be a result of the following Air bubbles in the flow cell Mobile phase
46. the vapors Sufficient Electrical Power The Series 200 Diode Array Detector requires a grounded nominal 100 120 220 or 230 240 Vac source Data processing equipment and other accessories require separate outlets Refer to the appropriate equipment manual for the power requirements To prevent degradation of the diode array detector s performance the AC line voltage must remain within 10 of the nominal specified voltage If you experience large voltage fluctuations install a voltage regulator between the diode array detector and AC outlet Solvents Mobile Phase Use only HPLC grade solvents in all analyses HPLC grade water and methanol 1 liter each are required for performance verification Series 200 Diode Array Detector User s Manual 2 3 Space Requirements The Series 200 Diode Array Detector is designed to sit on a benchtop it is normally plumbed into the LC autosampler as shown in Figure 2 3 A space approximately 20 cm 8 in high is sufficient for the detector if nothing else is to be placed over or on it Figure 2 3 shows the Series 200 Diode Array Detector in a typical LC system The case is 33 7 cm 13 25 in wide but additional space is required on both sides of the detector on one side for the tubing from the autosampler and on the other side for the tubing to the drain vessel The case is 80 5 cm 22 75 in deep but additional space is required behind the unit to accommodate the power cord and cables to
47. urbochrom Workstation User s Guide P N S270 1601 Series 200 Diode Array Detector Users Manual 3 5 From the Turbochrom Navigator screen select Method Editor and create a new method by selecting from the parameters and timed events listed below Setting the Autosampler Parameters 1 Select the Autosampler tab of the Instrument Control dialog box Autosampler Pump Config Pump Program Detectors Instrument Timed Events r Injection Source Derivatization Dilution Manual Off Deriv Dilution Program z Sample Injection z Flushes Injection volume uL 10 0 Flush volume uL fo Loop size pL 200 7 Flush speed Medium Fixed mode fo zx Flush cycles 2 Excess volume uL Fo Pre injection cycles h Air cushion pL fio Post injection cycles Mies Sample syringe size pL 250 v Post method cycles p Sample speed Medium gt Needle level fx o Peltier Tray Control Iniect delay time min oO Temperature C fa f fo Fe Cancel Help The autosampler will be used 2 If you are using manual injection select Manual If you are using an autosampler select Autosampler and continue with Steps 3 through 20 3 Inthe Injection Volume text box enter the amount of sample that you want to inject Make certain that this value is less than 50 of the Loop Size that you specify in step 4 4 From the Loop Size list select the size of the injecti
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