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User`s Manual Confocal Scanner Unit CSU-X1

1. Table 7 4 2 PCR68 pin male connector of Control unit Pin Signal name Function I O Connection No 1 TI1 E11 1 TTL input ch1 Input 2 TI2 EI2 1 TTL input ch 2 Input 3 TIS EI3 1 TTL input ch3 Input 4 TI4 EI4 1 TTL input ch4 Input 1 2 T4ACT541 AN 5 VCOM1 Source for contact Input For contact input source DC5V input VCOM1 6 VCOM2 Source for contact Input input VCOM2 vcom H 1 7 VCOM3 Source for contact Input pt CH sjy at input VCOM3 330 TLP115A 8 VCOM4 Source for contact Input input VCOM4 9 Interlock Open is Interlock Input Connect with 43pin 10 Reserved 11 Reserved 12 CSU SYNC CSU sync signal Input TTL CMOS level input 300Hz 2kHz limitation exists in the rotation speed s 13 SHUTTER 5 External shutter Input Pa er control signal H Open L Close aia 14 OC1 EO1 2 Open collector Output DC5V 100mA max outputl H is ON 15 OC2 EO2 2 Open collector Output 1 oc output 2 H is ON 16 0C3 Open collector Output output 3 His ON 3N75452B 17 OC4 Open collector Output l output 4 H is ON 18 DO1 EO5 Contact output 1 Output DC30V 500mA max 3 H is ON 19 DO2 EO6 Contact output 2 Output 5V 3 His ON Li 8 11 pot 20 DO3 EO7 Contact output 3 Output v 5 3 H is ON A i o JE Do 21 DO4 EO8 Contact output 4 Output Fl 3 His ON AQV2125 22 Reserved
2. Acquire serial number of CSU SERIAL_CSU Acquire product ID of CSU 73 PRODUCT CSU Error information Connection status Software information Hardware misc Command notation colon or space delimiter Acquire error ID ERRID Acquire hardware connections SYSTEM Acquire software version VER Acquire build time of software BULIDTIME Output error ID log LOG_ON Not to output error ID log LOG_OFF Acquire connection status of own control unit SYSTEM_THIS Acquire connection status of extension control unit ch channel number of connected instruments n integral number parameter f decimal number parameter xxx letter string parameter SYSTEM EXT 9 Warranty Contact The term of warranty is for one year after the day of purchase Yokogawa Electric Corporation will repair the Product at no charge during the term of warranty This warranty covers only this instrument Yokogawa Electric Corporation cannot be held liable for damages resulting from operating errors improper use handling or storage unauthorized repair or modification of this instrument by the customer transportation fire natural disasters such as earthquake flood thunderbolt etc salt damages corrosive gas damages or abnormal voltage moving or dropping this instrument after delivery or any other damages for which
3. lt i BA Filter Block 2 High end model 1 Take out filter wheel after removing the screw at the upper right side of filter block Please refer to the instruction manual of CSUX1FW for details 2 Remove cover and take out BA filter after removing fixing screw ring by using supplied filter wrench 3 Insert and fix new BA filter with fixing screw ring by using the filter wrench 46 CAUTION Do not touch and stain filter surface Be careful to set the filter surface at the right direction 4 Fix the cover Ad Warning NCAUTION Never inset your fingers in the open ports of filter wheel You could injure fingers if the wheel moves You must power off the filter wheel when you exchange filter otherwise you may injure fingers if the wheel moves by accident Please fix supplied dummy filter disks at each of open position so as to balance weight Without proper balance filter wheel may not work properly 5 2 Dichroic Mirror Unit Exchange Please ask for the advice form your support service if you wish to exchange the DM unit MN WARNING and PRECAUTION Never exchange the DM block when the laser beam is emitted The Class 3B laser radiation could be hazardous to the eyes if you directly look into the beam of reflected laser beam You should avoid exposure to the beam 47 6 Trouble Shooting Please always follow safety instructions for laser hazard CLASS 3B products when you inspect CSU X1probl
4. MN CAUTION There is a limitation in the operation interval of this filter wheel If moved too fast there could be malfunction Please refer to the specification list in this manual 13 4 Name and function 14 1 Incident light path window Do not put your fingers into this window when turn on the power supply and filter wheel is under operation You could injure your fingers 2 Outgoing light path window Do not put your fingers into this window when turn on the power supply and filter wheel is under operation You could injure your fingers 3 Connector for MOTOR cable Connect with the supplied MOTOR connecting cable Please connect MOTOR1 connecting cable to the 1st filter wheel and MOTOR2 connecting cable to the 2nd filter wheel 4 Filter wheel cover Please open when exchanging filters Make sure to put off power when you exchange filters 5 Filter cover fixing screw Four screws to fix the cover 6 Filter ports 7 Filter fixing ring Can be tightly screwed by using supplied screwing tool 20 15 8 6 ports filter table Filter location numbers from 1 to 6 are printed on the outer plate Please set supplied aluminum discs into any open ports for weight balance 9 Motor cover Do not remove this cover to avoid burn injury since surface of the motor could become hot under operation 10 Motor side pulley 11 Ww Motor fixing screws Never loosen these screws otherwise motor may fail normal fun
5. Basic model with BrightField High Speed 10000rpm 5000rpm Low Speed 1800rpm Table 7 2 2 CSU X1 Suffix Code DM Dichroic Mirror and BA Barrier Filter or fiber Selection Suffix Code Laser line Example of Dyes D 000 488nm EGFP FITC Dichroic D 001 532nm TRITC Mirror DM D 002 568nm DsRed mRFP D 100 445 488 ECFP EGFP FITC D 101 442 505 635nm ECFP EYFP Cy5 D 200 405 473 491 568 635 647nm DAPI EGFP FITC DsRed mRFP Cy5 D 201 445 514 635 647nm ECFP EYFP Cy5 BH100 405nm DAPI Barrier BH101 440 445nm ECFP Filter BA BH102 473 491nm EGFP FITC B 103 473 496nm ArKr laser EGFP FITC BH104 505nm 514nm EYFP BH105 532nm TRITC B 106 561nm 568nm DsRed mRFP BH107 635 647nm Cy5 Fiber FB1 405 650nm With the standard fiber means DM position O for M Manual Model 1 2 3 for A High end model to indicate DM position No duplicate numbers can be assigned means BA position 0 for M Basic model In the case of A High end model please refer to Suffix code of CSUX1 FW Filter wheel 58 Table 7 2 3 Basic Specification of Control Unit Model Basic Code CSUX1CU Filter Wheel Control Bright Field Control Main Unit Language of Japanese label English Table 7 2 4 Basic Specification of Filter Wheel Unit Model Basic Code CSUX1FW Filter 6 Positions posi
6. Response 10ms Filter Wheel Command control of filter positions control One control unit controls up to two filter wheels CSU X1CU F1 Bright Field Command control of light path switching confocal non confocal Camera port Switch between 1st camera port and 2nd camera port control CSU X1CU B1 Interlock key 1 Installed Manual shutter switch becomes valid External shutter control from PC through control unit becomes possible 2 Uninstalled Shutter closes motor stops and manual shutter switch becomes invalid 29 Communication 1 Connection Connect control unit cable to the external interlock connector of CSU X1 head Connect control unit and PC through RS232C serial cable Possible to connect and control two control units through daisy chain 1 Conditions Speed 115 200 bps Data bit 8 Parity check None Stop bit 1 Line feed character CR Separating character colon or space Q Case sensitivity Yes Commands Shown in the command list Power Input 100 240VAC 10 50 or 60Hz 200VAmax consumption Accessory 1 AC100VAC cable 2 Bright Field1connection cable only for CSU X1CU B1 3 Bright Field2connection cable only for CSU X1CU B1 4 CSU connection cable 5 RS232C cable cross 1 6 Interlock key for control unit 6 1 2 Filter Wheel Option Installable filters Size p25mm gt Max 6 filters It is necessary to fill open position with a dummy disc for weight balance Ex
7. User s Manual Confocal Scanner Unit CSU X1 IM 85A7C25 02E YOKOGAWA 9 IM 85A7C25 02E PAM ERIE 1st Edition TABLE OF CONTENT L Safety Precautions a AAA 5 dd Introduction sasinan A A A GT 5 T2 CAUTION aio 5 13 ELISCOVY A E A E E eects oes 5 1 4 General Safety Precautions siic dd 6 1 5 laser safet Precautions A ue ane 8 1 5 1 Laser Satety St ndardsussduu raser eee 8 1 5 2 About the Class 3B Laser cuina diia dit id its 11 1 5 3 Safety Precautions mar rtiene 11 2 gt Product Overview A A sadelen 18 2 1 Principle of the Confocal Microscope ccococcccnnnnnonnnnnnnonnnnnononnnnnoncnnnnnnnnnnnos 18 2 2 Structure and Features of the CSU X occcccccnnnnnnncnnnnncnnnononononononononos 19 23 Descnphon of CSU XI undra aner 20 2 3 1 OSUXE Mid O Saksen seo 21 2 3 2 CGSUXI Mt ene beast 22 2 3 3 CSU XLA T tardes 23 2 3 4 CSUX TEA mins et 24 2 3 5 ESTIMA a nd SERT 25 3 Before Using the UNE AS AA A ii 26 ool Packing Vist ii AA AA AAA AA in 26 32 Option litis ad ii 27 3 2 1 Filter wheel control unit CSU X1CU FD oocononncicnnininnononinncnnanininnccnnonininicnnanns 27 3 2 2 Filter wheel CSU X1FW mmmmnevesrersersvrrrrerveevrerrarsresvesverssnsveeveasseesvesrersessvssvssnne 27 3 2 3 Bright Field Control unit CSU X1CU BW aaa 27 O EE EEE aaa ia 28 3 3 1 Installation of Microscope cccccssssscecececeessssecececececeensssececececeeeeesesssaeeeeeeseeeneas 28 3 3 2 Installation of Laser Syst
8. e Bright Field means non confocal wide field images such as DLC phase epifluorescence and TIRF 29 2 3 3 CSU X1 A1 Microscope CSUXICU F Laser inlet FC connector shutter Excitation filter Option Dichroic mirror See Specification List Barrier filter See Specification List AmAOORe Camera port 8X8mm adapter for EMCCD is available Option G Direct view eyepiece unit Option K Dichroic mirror block automatic control for up to three dichroic mirrors L Filter Wheel for up to six barrier filters This is a High end model with motorized dichroic mirror unit for up to 3 dichroic mirrors and a filter wheel for up to 6 filters Disk rotation speed is adjustable between 1 500 10 000rpm CSU X1 A1H or between 1 500 5 000rpm CSU X1 A1N respectively if connected with the control unit CSU X1CU F1 23 2 3 4 CSU X1 A2 CSUX1CU B1 Microscope CSUX1CU F 1 Laser inlet FC connector shutter Excitation filter Option Dichroic mirror See Specification List Barrier filter See Specification List we eee Camera port 8X8mm adapter for EMCCD is available Option Direct view eyepiece unit Option Dichroic mirror block automatic control for up to three dichroic mirrors Filter Wheel for up to six barrier filters Motorized light path switching port 1 Motorized light path switching port 2 This model is CSU X1 A1 plus Bright Field port wh
9. 100 240VAC Power voltage frequency range 90 264VAC Rated supply frequency 50 60Hz Power frequency latitude 48 63Hz Maximum electric power consumption 200VA 12 Please follow the warnings and cautions below before connecting the power supply of a control unit If these warning and cautions were not followed there is a possibility of causing an electric shock or breakage of apparatus MN WARNING e Please confirm your power supply is suitable for the rated supply voltage of this unit before connection e Please confirm before connecting the power cord if the power switch of this unit is off e Please ONLY use supplied power code to prevent electrical shock or fire e Please be sure to ground the protective earth for electric shock prevention e Please always use 3 prong plug with protective earth terminal to avoid risk of electrification e Please do not use the extension code without a protective earth conductor Use of such will cancel out protection 13 CSU control cable i o Interlock key ferrite core at this side A y ha oca y Power cable RS232C cable PI Power cable gapu i i FW2 cable FW1 cable A CAUTION Please ONLY use attached cables otherwise it is possible to fail EMC conformity 14 3 3 2 2 CSUX1CU B1 Model Following connection is necessary to use Bright Field with CSUX1 A2 and A3 models 1 Connect RS232C2 connector of control unit 1 CSUX1CU F1 and
10. 23 A O NO 24 1 Safety Precautions 1 1 Introduction Thank you so much for purchasing the CSUX1FW Filter Wheel for CSU This manual covers the features operation methods and procedures safety and handling precautions of the CSUX1FW Before you start to use the unit read this manual thoroughly and operate the unit in a proper manner After read the annual through keep the manual in a safe place for future quick reference 1 2 Caution No part of the user s manuals may be transferred or reproduced without prior written consent from YOKOGAWA YOKOGAWA continues to improve the product s performance and features and for this reason the specifications and information herein are subject to change at any time and without notice The authors and publishers of this manual have used their best efforts in preparing this document but make no representation or warranties with respect to the accuracy applicability fitness or completeness of the contents of this manual If you have noticed any problems or have any suggestions for the manual please contact us To maintain long term stable performance of the product observe the operation instructions in this manual 1 3 History 1st Edition August 2007 1 4 General Safety Precautions For the protection and safe use of this instrument be sure to follow the instructions on safety described in this manual when handling this instrument If you use the instrument is a man
11. 23 Reserved 24 Reserved 67 25 Reserved 26 Reserved 27 CSU_INDEX CSU rotation sync Output TTL level signal 300 2kHz A 4 2 Sl 1 74ACT541 22 4 so D 28 31 Reserved 32 34 5V 5VDC Output 100mA max 35 GND GND 36 GND 37 GND 38 GND 39 DI1 EI5 4 Photo coupler input Input DC5V 7 20mA 1 40 DI1 EI5 4 Photo coupler input Input ycom al 1 an 2 O j 3 ya Sia 41 DI1 EI5 4 Photo coupler input Input 330 TUPTISA 3 42 DI1 EI5 4 Photo coupler input Input 4 43 Interlock Interlock Input Connect with pin 9 44 GND 45 GND 46 GND 47 GND 48 GND 49 GND 50 GND 51 GND 52 DO1 Contact output 1 Output DC30V 500mA max 53 DO2 Contact output 2 Output 54 DO3 Contact output 3 Output 5V 55 DO4 Contact output 4 Output I 11 no 4 DO AQV2125 56 68 GND 1 Possible to acquire input terminal ch1 ch4 levels EI ch 2 Possible to assign output terminal ch1 ch4 levels E0 ch n 3 Possible to assign output terminal ch5 ch8 levels EO ch n 4 Possible to acquire input terminal ch5 ch8 levels EI ch 68 MN WARNING the shutter A CAUTION 5 Please control shutter ON OFF below 3 Hz Faster ON OFF control may damage Please do not open the dichroic mirror block cover when you input shutter control signal If you d
12. B1 Pin No Function T O Connection 1 Unconnected 2 Unconnected 3 Unconnected 4 Unconnected 5 Unconnected 6 Unconnected 7 Unconnected 8 MOTOR loutput Output 9 MOTOR loutput Output 10 MOTOR output Output 11 Sensor input Input 12 MOTOR output Output 13 MOTOR output Output 14 Source for sensor 15 GND for sensor 71 Table 7 49 MOTOR2 D Sub15 pin connector for control unit 2 CSU X1CU B1 Pin No Function T O Connection 1 Unconnected 2 Unconnected 3 Unconnected 4 Unconnected 5 Unconnected 6 Unconnected 7 Unconnected 8 MOTOR output Output 9 MOTOR output Output 10 MOTOR output Output 11 Sensor input Input 12 MOTOR 1output Output 13 MOTOR output Output 14 Source for sensor 24V 15 GND for sensor 72 8 Command list Main Item Detailed item Function Command CSU control Shutter Open shutter SHO Close shutter SHC Acquire shutter status SH Acquire open close count SH_CNT Disk Assign disk rotation speed rpm MS n Fine adjustment of disk rotation MS speed to increase Fine adjustment of disk rotation MS speed to decrease Acquire disk rotation speed rpm MS Acquire upper limit of disk MS MAX rotation speed rpm Aut ti dj utoma aC adjustment to camera MS_ADJUST f exposure time ms Run disk rotation MS_RUN Stop disk rotation MS_
13. CSU control unit 1 This is not supplied if you already have aCSUX1CU F1 3 1 3 Option Filter Wheel CSUX1FW Necessary for CSUX1 A1 A2 A3 orCSUX1 M1N M1H M2N M2H Part name Part number QTY Description Filter wheel M3918MA 1 Filter wheel Aluminum disc for M3916MW 6 Aluminum disc to fill open positions in the filter weight balance wheel for weight balance Connection cable I M3918AP 1 Connection cable for filter wheel 1 sl 2 5m shield cable Connection cable 2 M3918AQ 1 Connection cable for filter wheel 2 1 2 5m shield cable Filter Wrench M3916CX 1 Tool for exchanging filters in the filter wheel 1 Connection cable 1 is supplied with CSUX1FW 06P 01 and Connection cable 2 is supplied with CSUX1FW 06P 02 MN CAUTION You have to use attached cables ONLY otherwise it is possible to fail EMC conformity 3 2 Precautions 3 2 1 Safety Precautions Before you start to use the unit please read this manual thoroughly Do not remove the cover to avoid risk of burn injury since some parts inside the unit could become high temperature Please contact Yokogawa Electric Corporation or its representative when you need inspection or adjustment of this instrument In case of such abnormal status as unusual odor or smoke goes up from this instrument turn off the power switch and disconnect power code Please contact Yokogawa Electric Corporation or its representat
14. EEE aida 7 21 NS a NE 7 3 Before Using the Unitaire cosssecedesstedsa codes cuecedecessasusctedesdhecccesdenebesseseedeede 8 31 gt Packing Listin eaten ds 8 3 2 Before Us A A A os ea eae Gav g bs soaks beens neg ieee 9 3 2 1 Safety PPECAULIONS isissss2c0 52 scneedeezeaboeaeanessecasacges nguavgnsesuacesdeauenseseedsa bbeaeaaeaesiaees 9 3 2 2 General precautions on handling ooonnnnnonnnnonnnooononononnnonononnnnononnnnnnnnnnnnnnnnnnos 9 BO Installation A sie oaaae a AGR 10 3 3 1 Installation requireMent ccceeeccccccccceeeesseeeeeccccceeeeeseeeeeseeeseeeuaeaeeeeseeees 10 3 3 2 Connection of equipment ccccccceccessseeeecccccceeussseeeecececeeeussseeeceeseeensaaeeesce 11 8 8 38 Application of POWE aroase iei iine a iaa iekala taiii 13 4 Name and functions ieie a a e det eo EE eA E E EE A E E datet 14 5 Maintenance ireen a A IA 17 A TS EA sass potaannndedessagddetunnsesecdagetes ANSES E Eaa 17 6 Speciheatiom asera ia ici 19 GTL Special A E sa old 19 6 1 1 Specification of filter wheel rrrrnnnnnnnnnnnnorrrnnnnnnrnnnnnerernnnnnnrnnnnnerernnnnnnnnnnnne 19 6 1 2 Enviroment eodera i ee T A aaa rattet 19 6 1 3 A 20 6 1 4 Global Environment Action osrnrnnornnnrnnnennnrrnnnennnrnnnenenrnnnenenrrsnseennnrnensnnnresee 20 6 2 Model and Suffix Code MS Codina ai nae 21 6 3 Dimension of CSUX1FW ccccecccccceccesseeneneeceeeceeeensneneeeeeeceesesseseaaeeseeeseeeeenea 22 6 4 Controla daa IIA dana
15. Olympus BX50 microscope Please make sure both windows eyepiece side and camera port side are sealed Furthermore it is strongly recommended that you should always shut CSU X1 shutter when you are not observing confocal images so that you can avoid accidental laser exposure Figure 3 2 Where to seal the 20 80 windows of the switching slider of the Olympus BX50 microscope When you switch the light path between confocal and non confocal observation epifluorescence transmitted light image etc reflected laser beam could reach into the microscope eyepiece at the moment of switching due to the reflection at the edge of switching prism Never look into the microscope eyepiece when you change the light path and always shut the CSU X1 shutter when you switch the light path or turn off the laser when you switch the light path 3 Laser warning labels Please attach laser warning labels on your microscope as indicated in Figure 1 3 3 3 2 Installation of Laser System and Adjustment of Fiber Coupler Please follow the instructions given in each of the user s manuals to install the laser system and fiber coupler and make adjustments so that the connection between the laser source and fiber coupler can be optimized MN WARNING You should strictly follow the laser hazard class 3B safety requirements whenever you install adjust or remove the fiber coupler from the laser system and connect or disconnect the fiber from the CSU X1 N
16. Varingen D8953RH 1 Don t look into the eyepieces when you change the light pass 1 LASER RADIATION AVOID Warning label M3916LK EXPOSURE TO BEAM CLASS 3B LASER PRODUCT Interlock key for 1 To control CSU X1 from the control CSU X1 unit bad panel Me M3916CV 1 For M3 set screw to fix a camera to C mount Botton porron D8953GV 1 a laser output hole mounted at actory 1 For second camera port mounted at a dad peso factory Bright Field or two FW type Second port cover screws 4 Could be mounted at factory Camera port 1 M3916JA 1 Camera port of CSU X1 Caer port apport M3916CT 1 Support when CSU X1 is installed at side port of inverted microscope Screw Y9510ZU 2 To fix M3916CT camera port support Key Switch 1 Spare key Optra fer M3916VU 1 Angled 8 degrees polished AFC connector A CAUTION Please only use CSU X1 specific optical fiber supplied with the CSU X1 head for the In case different optical fibers were used CSU X1 may best optical performance not be able to work at its best optical performance 26 3 2 Option List 3 2 1 Filter wheel control unit CSU X1CU F1 Part name Part number QTY Description Filter wheel control unit M3918EA 1 Main control unit Interlock cable M3918AN 1 2 5m shield cable for control unit Communication cable M3918WA 1 Crossing serial cable DSUB 9 pin female female 3m shield cable Controller Interlock key M3918AT 1 Interlock key for cont
17. a dust cover in case of storing or fiber exchange 8 Fiber guard Guard to protect fiber from mechanical damage 9 C mount Camera port Please use supplied C mount adapter D8953CF to set a camera on this port and fix with set screws 10 C mount adapter D8953CF 11 Port switching knob manual Knob to switch light path between 15 camera port pushed and 2 4 camera port pulled In the case of A2 and A3 models no manual knob is provided since they have motorized switching system 12 Dichroic mirror block cover This cover will be removed when to set or exchange the dichroic mirror block inside If you open this cover Class 3B laser beam could be emitted Warning sign to class 3B laser radiation to advise to avoid exposure to the beam is attached Please ask for the advice from your support service when you wish to exchange the DM block 13 Light path selecting block cover Light path selecting block to set either a dichroic mirror or reflection mirror inside If you open this cover Class 3B laser beam could be emitted Warning sign for class 3B laser radiation to ask avoiding exposure to the beam is attached Please ask for the advice from your support service when you wish to exchange the mirrors 14 BA Barrier filter block Barrier filter block will be set here When you remove this block for filter exchange please turn off the power for laser safety 14 1 Fixing screw for setting the BA filter block or filter wheel
18. but make no representation or warranties with respect to the accuracy applicability fitness or completeness of the contents of this manual If you have noticed any problems or have any suggestions for the manual please contact us To maintain long term stable performance of the product please observe the operation instructions in this manual 1 3 History 1st Edition September 2007 1 4 General Safety Precautions For the protection and safe use of this instrument be sure to observe the instructions on safety described in this manual when handling this instrument If you use the instrument in a manner not specified in this manual the protection provided by this instrument may be impaired In addition Yokogawa Electric Corporation assumes no liability for the customer s failure to comply with these requirements The following safety precaution symbols are attached to the instrument AN ZA warnine or MN CAUTION Both of the above two symbols indicate that important content is mentioned The symbols are given wherever you are required to refer to the manual in order to avoid personal injury and or damage to the instrument The following safety precaution symbols are used in this manual MN WARNING This symbol indicates that the operator must observe the explanation in the user s manual in order to avoid serious injury or death and or damage to the instrument MN CAUTION This symbol indicates that the operator must
19. mRFP BH107 635 647nm Cy5 21 6 3 Dimension of CSUX1FW 6 4 Control Signal Table 6 4 1 D Sub15 pin connector for filter wheel Pin No Function I O Connection 1 MOTOR input input 2 MOTOR input input 3 MOTOR input input 4 MOTOR input input 5 MOTOR input input 6 MOTOR input input 7 MOTOR input input 8 MOTOR input input 9 Unconnected 10 Unconnected 11 Sensor input Output 12 Unconnected 13 Unconnected 14 Source for sensor 24V 15 GND for sensor 23 7 Warranty The term of warranty is for one year after the day of purchase Yokogawa Electric Corporation will repair the Product at no charge during the term of warranty The warranty covers this instrument only Yokogawa Electric Corporation cannot be held liable for damages resulting from operating errors improper use handling or storage unauthorized repair or modification of the Product by the customer transportation fire natural disasters such as earthquake flood thunderbolt etc salt damages corrosive gas damages or abnormal voltage moving or dropping the Product after delivery or any other damages for which Yokogawa Electric Corporation is not responsible Please contact either Yokogawa Electric Corporation or your representative if you want to request for repair Contact Yokogawa Electric Corporation Life Science Business Headquarters Bio Center E Mail cs
20. mount Total width of CSU X1 M2 main unit is 328 5mm including 4mm of the screw head of C mount adapter of main camera port Total height of CSU X1 M2 main unit is 308 5mm including 4mm of the screw head of C mount adapter of second camera port Please connect CSU X1 M2 to microscope with microscope specific adapter made by Yokogawa and supplied as option 64 7 3 6 CSU X1CU 7 3 7 CSU X1FW Unit mm 65 7 4 Control Signal Table 7 41 PCR28 pin male connector of CSU X1 main unit Pin No Signal Name Function I O Connection 1 Reserved 2 Reserved 3 SHUTTER 1 High Open Low Close Input TTL CMOS level 4 Reserved 5 Reserved 6 Reserved 7 SYNC Sync trigger signal input Input TTL CMOS level 8 INDEX Sync trigger signal output Output TTL CMOS level Pulse width 10ms lt 300Hz 2kHz 9 Interlock Open for Interlock Input TTL CMOS level 10 Reserved 11 Reserved 12 Reserved 13 PORT M6 port position signal Output TTL CMOS level 14 Reserved 15 Reserved 16 Reserved 17 Reserved 18 Reserved 19 Reserved 20 Reserved 21 SYNC GND 22 INDEX GND 23 Interlock GND 24 Reserved 25 Reserved 26 Reserved 27 PORT GND 28 Reserved 1 For the protection of shutter maximum allowable On Off frequency is 3Hz If you input faster signal shutter may not work properly 66
21. position in the filter slot at each of 1st and 2nd camera port Filter size p25mm 2 High end Model To use Filter Wheels installed in the filter slot at each of 1st and 2 4 camera port CSU X1FW 06P 01 Filter Wheel for 1st camera port CSU X1FW 06P 02 Filter Wheel for 274 camera port CSU X1CU F1 control unit is required to use CSU X1FW 06P 01 1 Light path exchange High end mode Motor driven Requires CSU X1CU B1 Bright Field control unit 2 Light path exchange Basic mode Manually exchange two ports with each lever 1 Standard C mount of each microscope 2 Option for Bright Field Microscope specific connectors are necessary to install Bright Field unit Specific connectors for Olympus Nikon Zeiss Leica upright inverted microscopes are available as option Light path exchange High end model Motor driven Requires CSU X1CU B1 Bright Field control unit 2 Light path exchange Basic model Manual exchange with a port exchange lever 1 Standard 1st camera port C mount X 1 2nd camera port position covered 2 Option 2nd camera port with C mount X 1 C mount adapter for EMCCD X 1 2 for 8x8mmCCD chip available for both 1st 2nd camera port Eye piece for direct view of confocal image at 2n1 port WARNING To install the direct view eye piece appropriate barrier filter to cut the excitation laser has to be installed for laser safety 51 Accessory AC adapter AC adapter cable for AC100V Las
22. seems to be defective Please confirm protective function before you use this instrument Use under gas It is extremely dangerous to use this instrument under the atmosphere with explosive or evaporating gas or vapor Disassembly of the case There are high voltage parts inside this instrument Never disassemble the case except by the authorized service person External connection Ensure the protective earth before connecting to this instrument CAUTION This instrument is used in combination with CSU X1 confocal scanner unit filter wheel laser system microscope and cameras to be purchased separately In addition to this manual read carefully the user s manual of the respective products and follow their instructions To avoid damage to this instrument make sure to use input and output terminals within their specification This instrument is a precision optical instrument Do not use this instrument in areas with excessive vibration or dust high humidity excessive heat near heat sources or under direct sunlight sudden temperature change where condensation could occur corrosive or flammable gas etc In case of mechanical trouble never touch inside the instrument and contact Yokogawa Electric Corporation or its representative 2 Product Overview 2 1 Product Overview CSUX1CU controls Confocal Scanner Unit Model CSU X1 Major functions of CSUX1CU are shown below CSU scanning speed Max speed varies dependi
23. to improve the product s performance and features and for this reason the specifications and information herein are subject to change at any time and without notice The authors and publishers of this manual have used their best efforts in preparing this document but make no representation or warranties with respect to the accuracy applicability fitness or completeness of the contents of this manual If you have noticed any problems or have any suggestions for the manual please contact us To maintain long term stable performance of the product please observe the operation instructions in this manual 1 3 History 1st Edition August 2007 1 4 General Safety Precautions For the protection and safe use of this instrument be sure to follow the instructions on safety described in this manual when handling this instrument If you use the instrument in a manner not specified in this manual the protection provided by this instrument may be impaired In addition Yokogawa Electric Corporation assumes no liability for the customer s failure to comply with these requirements The following symbols are used on the instrument A A WARNING or AN CAUTION Both of the above two symbols indicate that important content is mentioned The symbols are given wherever you are required to refer to the manual in order to avoid personal injury and or damage to the instrument The following symbols are used in this manual MA WARNING Th
24. unit 15 Camera port support To adjust height and support weight when CSU X1 is installed at the side port of microscope 49 16 C mount port The port to connect C mount adapter of your microscope Dust cover D8953GV is fixed at factory 17 Dust cover for 2nd camera port will be provided with M1 and A1 models 18 Space for excitation filter block The same excitation block with that of CSU10 can be inserted here 19 Identification label MS code rated supply voltage product number and serial number 20 Cover for the excitation light path This cover may be removed when you install the excitation filter unit If you open this cover Class 3B laser beam could be emitted Warning sign to class 3B laser radiation to ask avoiding exposure to the beam is attached Please ask for the advice from your support service when you wish to install or exchange the excitation filter unit Please refer to Chapter 1 for the explanation of warning labels 43 4 2 M2 Model Please refer to 4 1 for common parts 21 Bright Field unit Only for M2 and A2 models 22 Bright Field switching knob Switch the light path between confocal pushed and bright field non confocal pulled In the case of A2 model port switching is automated and no manual knob is provided 23 Microscope adapter Microscope specific adapters are necessary to install Bright Field Option 4 3 A1 Model 24 Filter Wheel 1st
25. xxx letter string parameter 35 7 Warranty e The term of warranty is for one year after the day of purchase Yokogawa Electric Corporation will repair the Product at no charge during the term of warranty The warranty covers this instrument only Yokogawa Electric Corporation cannot be held liable for damages resulting from operating errors improper use handling or storage unauthorized repair or modification of the Product by the customer transportation fire natural disasters such as earthquake flood thunderbolt etc salt damages corrosive gas damages or abnormal voltage moving or dropping the Product after delivery or any other damages for which Yokogawa Electric Corporation is not responsible Please contact either Yokogawa Electric Corporation or your representative if you want to request for repair Contact Yokogawa Electric Corporation Life Science Business Headquarters Bio Center E Mail csu csv yokogawa co jp Tel 81 0 76 258 7028 Fax 81 0 76 258 7029 36 User s Manual Filter Wheel CSUX1FW IM 85A7C27 01E YOKOGAWA 4 IM 85A7C27 01E PAM ERIE 1st Edition Table of contents 1 Safety PTECAULIONAS AA eae ean sacs eww eA OREA 3 LD gt Tntroductionsaussgertevultsdsrendlidk aids letet lede 3 A TN 3 Lo e HISTOL NN 3 1 4 General Safety Precautions cccccccccccesccesceeseeesccesceeseeeeceeeeeeeeeeeeeeseseseeeeeeeeeess 4 2 Product Dv Wi A age a E E EAE
26. 0kg Bright Field Unit 2kg Control unit CSU X1CU 5 2kg Filter wheel CSU X1FW 1 9kg 1 Desirable environment for delivery storage or whatever when the instrument is not in operation under which the instrument may not suffer unrecoverable damages 24 6 1 4 Conformity CE marking 1 9 EMC Directive EN61326 EN61000 3 2 EN61000 3 3 Low Voltage Directive EN61010 1 Measurement category 1 Pollution Degree 2 2 To measure circuit connected to low voltage units applied on electrical instruments which has power supply from fixed power switchboard Degree of adhesion of any solid liquid or gas which may lower surface resistance or electric strength applied to normal room atmosphere Global Environment Action Lead free solder is used Lead free glass is used No use of Cr 6 for chromium plating 25 6 2 Model and Suffix Code MS Code Basic specification code of control unit Model Basic Code CSUX1CU For Filter Wheel For Bright Field Language of Japanese labels English Main Unit Basic specification of Filter Wheel Option Please refer to IM of CSUX1FW 6 3 Dimension of CSUX1CU 132 20 26 6 4 External Input Output Table 6 4 1 PCR28 pin male connector Pin No Signal Function T O Connection 1 Reserved 2 Reserved 3 SHUTTER 1 Shutter control input Inp
27. 1 at the time of installation or storing In the case of malfunction please do not open the CSU X1 but consult Yokogawa Electric Corporation or your local representative for proper advice and repair 1 5 Laser safety Precautions Laser products are classified on the basis of their laser beam wavelengths and power characteristics and a common set of safety standards is applied to the products in each class The CSU X1 is classified as a laser hazard CLASS 3B instrument in accordance with WHO regulations which indicates that it is capable of producing skin or eye damage if used improperly Direct viewing into the laser beam or the viewing of reflected beams must strictly be prevented The WHO recommendations concerning health and industrial protection when handling laser instruments must strictly be observed Before operating the CSU X1 please read the following to become familiar with its safety control markings and warnings 1 5 1 Laser Safety Standards The CSU X1 is designed in compliance with the Emission Safety Guideline of Laser Product of Japanese Industrial Standard JIS C 6802 For safe use of the CSU X1 the following considerations are made AN Warning labels You must attach the warning labels at appropriate positions indicated below either on the CSU X1 or on the microscope at installation and strictly follow the warnings when using the CSU X1 for your own safety Figure 1 1shows warning labels for safe use of the lase
28. 12 W x100 H x226 L mm w o protruding parts Weight 1 CSU X1 Main unit 5 5kg Camera port and filter block 2 0kg Bright Field Unit 2kg Control unit CSU X1CU 5 2kg Filter wheel CSU X1FW 1 9kg 1 Desirable environment for delivery storage or whatever when the instrument is not in operation under which the instrument may not suffer unrecoverable damages 56 7 1 6 Conformity CE marking 1 9 EMC Directive EN61326 EN61000 3 2 EN61000 3 3 Low Voltage Directive EN61010 1 Measurement category 1 Pollution Degree 2 2 To measure circuit connected to low voltage units applied on electrical instruments which has power supply from fixed power switchboard Degree of adhesion of any solid liquid or gas which may lower surface resistance or electric strength applied to normal room atmosphere FDA Laser Safety Regulation Applied on August 2007 7 1 7 Global Environment Action Lead free solder is used Lead free glass is used No use of Cr 6 for chromium plating Will be completed by March 2008 57 7 2 Model and Suffix Code MS Code Table 7 2 1 CSU X1 Basic Specification Codes Model Code CSU X1 eevee Confocal scanner unit model CSU X1 Rotation speed Language labels 1800 rpm is for CSU X1 M1 and M2 ONLY High end Model Basic Model Standard Japanese English Specification High end Model with BrightField High end model with Second camera
29. 32C2 CH2 if you use two control units Please firmly connect with fixing screw 10 RS232C2 connector For serial interface CH2 connection using supplied RS232C cable It is not necessary when you use only one control Connect to RS232C1 CH1 of the second control unit if you use two control units Please firmly connect with fixing screw 11 MOTOR1 connector CSUX1CU F1 model Connector for Filter wheel 1 connected with supplied FW1 cable Please firmly connect with fixing screw CSUX1CU Blmodel Connector for Bright Field1 connection connected with supplied Bright Field1 cable Please firmly connect with fixing screw 12 MOTOR2 connector CSUX1CU F1 model Connector for FW1 connection connected with supplied FW1 cable Please firmly connect with fixing screw CSUX1CU Blmodel Connector for Bright Field2 connection connected with supplied Bright Field2 cable Please firmly connect with fixing screw 13 Identification label MS code rated supply voltage product number and serial number 19 5 Maintenance e Never use organic solvent to clean this instrument Wipe off stains with clean cloths and if necessary diluted detergent may be used to remove stubborn stains e Consult Yokogawa Electric Corporation or your local representative in the case of malfunction 5 1 Circuit Breaker 1 Position Circuit breaker instead of fuse is installed at the following position Circuit breaker 2 Function Shut down
30. C30V 500mA max 53 DO2 Contact output 2 Output 5V 54 DO3 Contact output 3 Output Li p i 55 DO4 Contact output 4 Output pr 5 1 AQV2125 56 68 GND 1 Possible to acquire input terminal ch1 ch4 levels EI ch 2 Possible to assign output terminal ch1 ch4 levels EO ch n 3 Possible to assign output terminal ch5 ch8 levels EO ch n 4 Possible to acquire input terminal ch5 ch8 levels EI ch 20 N WARNING Z CAUTION B Please control shutter ON OFF below 3Hz Faster ON OFF control may damage the shutter Shutter may not work properly above 3Hz Please do not open the dichroic mirror block cover when you input shutter control signal If you do so shutter closing timing could delay and laser beam may leak out Table 6 4 3 PCR36 pin male connector Pin No Signal Name Function T O Connection 1 AD1 1 Analogue input 1CH Input Resolution 0 5V 10bit 2 AD2 1 Analogue input 2CH Input Precision 8bit A 3 AD3 1 Analogue input 3CH Input OT 4 AD4 1 Analogue input ACH Input 4 5 AD5 1 Analogue input 5CH Input m To 6 AD6 1 Analogue input 6CH Input 22 x T AD7 1 Analogue input 7CH Input 193 8 AD8 1 Analogue input 8CH Input Naa Noa 9 DA1 2 Analogue output 1CH Output Resolution 0 5V 12bit 10 DA2 2 Analogue output 2CH Output Precision 8bit wy 11 DA3 2 Analogue output 3CH Out
31. Interlock key RS232C cable Power code Filter wheel cable 2 FW2 MN CAUTION Please ONLY use supplied cables otherwise it is possible to fail EMC conformity 34 6 Connection with control unit CSU X1CU B1 To use CSU X1 A2 or A3 you have to connect Bright Field control unit CSU X1CU B1 to CSU X1 a Connect RS232C2 connector of CSU X1CU F1 to RS232C1 connector of the second control unit CSU X1CU B1 with supplied cable b Insert interlock key into EXT INTERFACE1 T LOCK c Connect MOTOR of the second control unit CSU X1CU B1 with BF1 connector of CSU X1 using BF1 cable and MOTOR 1 with BF2 connector using BF2 cable respectively d In the case of CSU X1 A3 only connect MOTOR1and BF1 connector of CSU X1 e Connect power cable after confirming the power switch is OFF Please use power receptacle with the following specification 3 prong plug with protective earth terminal Rated supply voltage 100 240VAC Voltage frequency range 90 264VAC Rated supply frequency 50 60Hz Supply frequency tolerance 48 63 Hz Max power consumption 200VA MN WARNING e Please confirm your power supply is suitable for the rated supply voltage of the CSU X1 before connection Please confirm all main switches of CSU X1 and attached instruments are OFF before connecting power cord eo Please only use provided power cord to avoid any risks such as fire or electrification e Please always use 3 prong plug with prot
32. ONLY connectable with CSU X1 M1H or MIN Laser inlet FC connector shutter Excitation filter See Option List Dichroic mirror See Specification List Barrier filter See Specification List we Se Camera port 8X8mm adapter for EMCCD is available Option G Direct view eyepiece unit Option CSU X1 M1 is a Basic model Your choice of one dichroic mirror and one barrier filter can be installed at your order Options shown above are selectable Maximum disk rotation speed for CSU X1 M1L is 1 800rpm If connected with the control unit CSU X1CU F1 rotation speed of CSU X1 M1H is adjustable between 1 500 10 000rpm and that of CSU X1 M1N is between 1 500 5 000rpm respectively 21 2 3 2 CSU X1 M2 Microscope Control unit CSU X1CF F1 is ONLY connectable with CSU X1 M1H M1N Laser inlet FC connector shutter Excitation filter Option Dichroic mirror See Specification List Barrier filter See Specification List JERR Camera port 8X8mm adapter for EMCCD is available Option G Direct view eyepiece unit Option H Bright Field Unit I Manual Light Path Switching port J Manual Light Path Switching port This model is CSU X1 M1 plus Bright Field port which is an emission light path to bypass the disks in the CSU X1 and allows you to use the same camera for both confocal and non confocal 1 imaging without light loss Light path can be switched manually by moving the two ports I amp J
33. RS232C1 connector of control unit 2 CSUX1CU B1 with supplied cable 2 Connect controller interlock key to EXT INTERFACE1 1 LOCK 3 Connect MOTOR1 of control unit 2 CSUX1CU B1 and BF1 connector of CSU X1 with supplied Bright Field cable BF1 4 Connect NOTOR2 and BF2 connector with supplied Bright Field 2 BF2 cable Not necessary for A3 model 5 After checking the power switch is OFF connect the power cable Please use the power receptacle which fulfills the following conditions 3 pole electric socket equipped with protective earth terminal Rated supply voltage 100 240VAC Power voltage frequency range 90 264VAC Rated supply frequency 50 60Hz Power frequency latitude 48 63Hz Maximum electric power consumption 200VA Please follow the warnings and cautions below before connecting the power supply of control unit If these warning and cautions were not followed there is a possibility of causing an electric shock or breakage of apparatus AN WARNING e Please confirm your power supply is suitable for the rated supply voltage of this unit before connection e Please confirm before connecting the power cord if the power switch of this unit is off e Please ONLY use supplied power code to prevent electrical shock or fire e Please be sure to ground the protective earth for electric shock prevention e Please always use 3 prong plug with protective earth terminal to avoid risk of electrification e Ple
34. STOP Dichroic mirror Assign dichroic mirror position DM_POS n Acquire dichroic mirror position DM_POS Filter Wheel control Assign Filter Wheel position FW_POS ch n Acquire Filter Wheel position FW_POS ch Bright Field control Port switching control External lO control Move Bright Field port to the Bright Field Da 7 BF_INIT original point Switch port to confocal position BF_OFF Switch port to Bright Field BF ON position Acquire current position BF POS Port Assign Port position PT POS ch n Acquire Port position PT POS ch General purpose 10 Assign output terminal level O ch n Acquire appointed level of EO ch output terminal Acquire input terminal level EI ch General purpose DA General purpose DA General purpose AD Set DA output parameter Set DA output parameter DA DATA ch n DA DATA ch nl Acquire AD input value AD DATA ch Memory control Hardware information Save memory Save parameters SAVE MEM Save Filter Wheel information max 16 letters FW INFO ch n KXXX Save dichroic mirror information max 16 letters DM INFO In XXXX Memory load CSU Information Load parameters from memory LOAD MEM Load default parameter LOAD INIT Acquire Filter Wheel information FW INFO ch n Acquire dichroic mirror information DM_INFO ch
35. Yokogawa Electric Corporation is not responsible Please contact either Yokogawa Electric Corporation or your representative if you want to request for repair Yokogawa Electric Corporation Life Science Business Headquarters Bio Center E Mail csu csv yokogawa co jp Tel 81 0 76 258 7028 Fax 81 0 76 258 7029 75 User s Manual CSU Control Unit CSUX1CU IM 85A7C26 02E YOKOGAWA IM 85A7C26 02E BOT EPRI A 1st Edition Table of content 1 Safety PrECAULIONS id AA aaa aa ace eines 3 LD gt O NO 3 A O soutadaceasdasadstendoracadaddcontanedosad sogesdoutadseseaistaveounneestatstecaatensteecius 3 Lo e HISTOL NN 3 1 4 General Safety Precautions ccccccccsccesccssceeeceeecceseceseeeeceeseeeseeeeeeeeeeeseeeeeeeeeees 4 2 Product Overviewmusnsrsensudri tener eee eee 7 21 As A GSE 7 Br Before Users ka 8 Bil Packie List kg te tind dee a Ea E AEEA a a tas 8 3 1 1 CSU Filter Wheel Control Unit CSUX1ICU F1 oocononnncnicniciononocncnnnncnconcnos 8 3 1 2 CSU Bright Field Control Unit CSUX1CU B1 ooocncncononicionoccnnconononncnnoncnos 8 3 1 3 Option Filter Wheel CSU RT E VI naadde 9 3 2 E RN 10 3 2 1 Safety PRA os 10 3 2 2 General precautions on handling ooonnnnnnnnononnnonooonononoonono nono nono nro nn nonononnnnnnos 10 3 9 Installation A A A A A A dada 11 3 3 1 Installation requirementS cccccnnnononncooncnnnnnnonanacnononononnnonnnncononnnnnnnnnnnninonononns 11 IN A RO sedate ste
36. age the unit e Be careful not to add mechanical shock or drop the unit which could result in severe damage Application of mechanical shock to the cable could cause electric noise which may lead to malfunction e Avoid bringing any charged object close to the terminal which may cause failure e Please be most careful when you carry this unit Remove the connecting cable first then use the handle on this unit to carry This unit weighs about 2kg e Please do not wipe the plastic surface with organic solvents such as benzene or paint thinner which may cause discoloration When the surface of the unit gets dirty please clean with soft cloth wet with dilute solvent and then wipe with a dry cloth 3 3 Installation 3 3 1 Installation requirements Please use this unit under the following environment Ambient air temperature 15 40 Ambient humidity 20 75 RH No dew condensation MN CAUTION e Dew formation inside the unit may occur when you move the unit between much different temperature humidity environments In such case please wait for an hour or more until condensation resolved before use lt Ventilation gt Please be careful to secure enough space around the ventilation hole of the unit placed at the motor cover to avoid overheating MN CAUTION Please do not install the unit under the following conditions e Under direct sunlight e Close to heating objects e Polluted with oily smoke steam dusts or corrosive ga
37. ase do not use the extension code without a protective earth conductor Use of such will cancel out protection 15 Control unit 1 RS232C cable connect with RS232C2 of e control unit 1 CSUX1CU F1 Power code Bright Field2 BF2 cable Bright Field 1 BF1 cable A CAUTION Please ONLY use attached cables otherwise it is possible to fail EMC conformity 16 3 3 3 Power On 1 2 Control Unit Please confirm if the controller Interlock key is inserted into EXT INTERFACE1 I LOCK at the backside of the control unit If not please insert supplied key Also please confirm if CSU X1 and the control unit is connected with supplied cable And then put the power of the control unit ON CSU X1 Please switch on the CSU X1 after the control unit is ON 17 4 Name and Function 18 1 Power Switch 2 Power ON indicator Green LED lights when power is ON LED is off when power is OFF 3 AC Inlet Socket for AC POWER Please use supplied power cable 4 Circuit breaker 3A 5 Cooling fan 6 CSU connector To connect with CSU X1 with supplied CSU connector cable 7 EXT INTERFACE1 I LOCK connector For controller interlock and external input output connection 8 EXT INTERFACE2 connector For external analogue input output connection 9 RS232C1 connector For serial interface CH1 connection using supplied RS232C cable When you use one control unit connect to PC and connect to RS2
38. ble for the rated supply voltage of this unit before connection Please confirm before connecting the power cord if the power switch of this unit is off Please ONLY use supplied power code to prevent electrical shock or fire Please be sure to ground the protective earth for electric shock prevention Please always use 3 prong plug with protective earth terminal to avoid risk of electrification Please do not use the extension code without a protective earth conductor Use of such will cancel out protection 11 CSU control cable ferrite core at this side PC COM port Ca Interlock key AAK aa 2 wii ij 7 Filter Wheel 2 FW2 cable Filter Wheel 1 FW1 cable MN CAUTION Please ONLY use attached cables otherwise it is possible to fail EMC conformity 19 3 3 3 Application of Power e Application of power on the controller When you connect the CSUX1FW with a CSU X1 confirm if the interlock key is connected to EXT INTERFACE1 LOCK at the back of a controller and if not please connect the interlock key After checking the CSUX1 and the controller 1s connected with the connection cable turn on the controller power e Application of power on the CSUX1 Release of the interlock Please turn on power switch of the controller CSUX1CU F1 first then turn on the CSU X1 power A WARNING Never inset your fingers in the open ports of filter wheel You could injure fingers if the wheel moves
39. camera port Provided with Al A2 and A3 models Up to 6 44 emission filters can be installed 4 4 A2Model 25 Light path switching unit Motorized unit for automated light path switching provided for A2 and A3 models 4 5 A3 Model 26 Filter wheel for 29d camera port Provided with A3 model Up to 6 emission filters can be installed 27 C mount for 2 4 camera port To install a camera to the 2 4 port with supplied adapter D8953CF Dust cover D8953M7 is fixed at factory Please use dust cover when you don t use the 2 4 port 45 5 Maintenance Never use organic solvent to clean the CSU X1 body Wipe off stains with clean cloths and if necessary diluted detergent may be used to remove stubborn stains Always cover apertures of the CSU X1 C mount and camera port with the provided dust covers when not in use Never open or touch the inside of the CSU X1 Please consult Yokogawa Electric Corporation or your local representative in the case of malfunction 5 1 BA Filter Exchange 1 Basic model 1 Take out the BA filter block after loosening the fixing screw 2 Take out BA filter after removing fixing screw ring by using supplied filter wrench 3 Insert and fix new BA filter with fixing screw ring by using the filter wrench CAUTION Do not touch and stain filter surface Be careful to set the filter surface at the right direction 4 Fix filter block and tighten the fixing screw Fixing screw
40. change speed 33msec to the adjacent position Itis necessary to wait for 33msec lt before moving to the next position 66msec to move two positions It is necessary to wait for 33msec lt before moving to the next position 99msec to move three positions Itis necessary to wait for 33msec lt before moving to the next position Control unit makes the filter wheel move toward shortest direction Accessories 6 Aluminum disks dummy filter to balance weight Special tool to screw unscrew filter fixing rings Connection cable Supplied from the control unit 23 6 1 3 Environment Specification Environment 1 Operation temperature and humidity range 15 40 20 75 RH No condensation General environment 20 70 C 5 95 RH No condensation 1 Dimensions 1 CSU X1 Unit CSU X1 M1 175 W x328 5 H x177 5 L mm w o protruding parts CSU X1 M2 258 8 W x373 H x177 5 L mm Includes Bright Field w o protruding parts CSU X1 A1 175 W x328 5 H x325 1 L mm Includes filter wheel w o protruding parts CSU X1 A2 258 8 W x373 H x325 1 L mm Includes filter wheel and Bright Field w o protruding parts CSU X1 A3 308 5 W x328 5 H x325 1 L mm Includes 2 4 camera port and filter wheel w o protruding parts Control unit CSU X1CU 213 W x132 H x438 L mm w o protruding parts Filter wheel CSU X1FW 112 W x100 H x226 L mm w o protruding parts Weight 1 CSU X1 Main unit 5 5kg Camera port and filter block 2
41. coverable damages 19 6 1 3 Conformity CE marking EMC Directive EN61326 EN61000 3 2 EN61000 3 3 Low Voltage Directive EN61010 1 Measurement category 1 Pollution Degree 2 2 1 To measure circuit connected to low voltage units applied on electrical instruments which has power supply from fixed power switchboard 2 Degree of adhesion of any solid liquid or gas which may lower surface resistance or electric strength applied to normal room atmosphere 6 1 4 Global Environment Action Lead free solder is used Lead free glass is used 20 6 2 Model and Suffix Code MS Code CSUX1 Basic Specification Optional Please refer to Installation Manual for the CSUX1 Control unit Basic Specification Optional Please refer to Installation Manual for the CSUX1CU Basic Specification of Filter Wheel Model Basic Codes CSUX1FW Filter position 6 Positions First Camera Port Channel No Second Camera Port Please select filters enlisted below When you order filters please indicate filter position by numbers 1 to 6 and be careful not to select same numbers which means duplicate position Suffix Code Laser nm Dye Examples B 100 405nm DAPI Barrier Filter B 101 440 445nm ECFP BA B 102 473 491nm for Solid laser EGFP FITC B 103 473 496nm for ArKr laser EGFP FITC BH104 505nm 514nm EYFP BH105 532nm TRITC BH106 561nm 568nm DsRed
42. ction 12 Ww Belt adjustment screw Please do not touch these screws 13 Belt 14 Photo sensor 15 Photo sensor fixing screw Do not loosen these screws otherwise photo sensor may not work properly 16 Photo sensor cable fixing bracket Please do not remove this bracket otherwise cable and belt may touch and cause damages 17 Ww Name plate to show below Model code Rated supply voltage Product number Serial number 18 Warning label Warning label to indicate the operator must not insert fingers into the filter wheel ports under operation A WARNING Never inset your fingers into the open ports of filter wheel You could injure fingers if the wheel moves 19 Identification label To identify filter wheels 1 and 2 20 Ring screwing tool Special tool to screw unscrew filter fixing ring used when exchanging filters 16 5 Maintenance Please do not use organic solvents for cleaning painted surface of this product Please use soft cloth for cleaning Do not touch inside the unit in case of failure and please contact your local representative fro repair 5 1 Filter exchange 1 Filter exchange Set the notch of screwing tool t at the notch of filter fixing ring turn counterclockwise to loosen and remove the ring The notch on the tool matches with the notch on the ring 17 A WARNING Please make sure to turn off power when you exchange filters Otherwise you may get in
43. d as a laser hazard CLASS 3B instrument in accordance with WHO regulations which indicates that it is capable of producing skin or eye damage if used improperly Direct viewing into the laser beam or the viewing of reflected beams must strictly be prevented The WHO recommendations concerning health and industrial protection when handling laser instruments must strictly be observed 1 5 3 Safety Precautions 1 Controlled access area The laser products shall be operated within controlled access areas 2 Warning Signs At the entrance of the controlled access areas post laser warning signs 3 Surface reflection Metallic and other lustrous and reflective surfaces may reflect laser beams Make sure that no unexpected reflection of laser beams may occur 4 Beam path The laser beam coming through the optical fiber is injected into the CSU X1 via an FC connector Never remove the FC connector connected to the CSU X1 unless the outgoing laser beam is stopped Do not directly view or touch the laser beam source optical fiber or laser beam coming out of the CSU X1 5 Protective glasses When you want to observe the direct light or reflected light protect your eyes by wearing protective glasses 6 Key control of laser sources Power supply of laser system used for laser confocal scanner system can be turned on off by using a key provided with the laser system When you don t use the laser system please remove the laser key and store i
44. e p25mm gt Max 6 filters It is necessary to fill open positions with supplied dummy discs for weight balance Exchange speed 33msec to the adjacent position It is necessary to wait for 33msec lt before moving to the next position 66msec to move two positions It is necessary to wait for 33msec lt before moving to the next position 99msec to move three positions It is necessary to wait for 33msec lt before moving to the next position Control unit makes the filter wheel move toward shortest direction Accessories 6 Aluminum disks dummy filter to balance weight Special tool to screw unscrew filter fixing rings Connection cable Supplied from the control unit 55 7 1 5 Environment Environment 1 Operation temperature and humidity range 15 40 20 75 RH No condensation General environment 20 70 C 5 95 RH No condensation 1 Dimensions 1 CSU X1 Unit CSU X1 M1 175 W x328 5 H x177 5 L mm w o protruding parts CSU X1 M2 258 8 W x373 H x177 5 L mm Includes Bright Field w o protruding parts CSU X1 A1 175 W x328 5 H x325 1 L mm Includes filter wheel w o protruding parts CSU X1 A2 258 8 W x373 H x325 1 L mm Includes filter wheel and Bright Field w o protruding parts CSU X1 A3 308 5 W x328 5 H x325 1 L mm Includes 294 camera port and filter wheel w o protruding parts Control unit CSU X1CU 213 W x132 H x438 L mm w o protruding parts Filter wheel CSU X1FW 1
45. e be most careful to avoid damaging the optical parts Small particles originating from the base plate of dichroic mirrors could be visualized with highly sensitive cameras as minor artifact images Problem Possible Cause Solution Shutter LED Shutter external Set shutter external signal to Low or stop signal input stay lighted control signal is at against manual switch off or command off High Shutter LED Interlock is working Connect interlock key if not blinks Repeat Attach DM block cover properly blinking twice and off cycle Shutter LED Total on off cycle of Blinking stops by manual button Shutter works blinks Repeat shutter surpassed accordingly blinking 5 beyond guaranteed Please call you local rep and exchange the shutter if times and off cycle 60 000 times this problem occurs 49 7 Specification 7 1 Product Specifications 7 1 1 Specifications of CSU X1 unit Scanning speed 1 Basic model Standard 1 1 800rpm w o control unit Standard 2 1 500 5 000rpm Max 1000fps requires control unit High speed 1 500 10 000rpm Max 2000fps requires control unit 2 High end model with control unit Standard 1 500 5 000rpm Max 1 000fps High speed 1 500 10 000rpm Max 2 000fps Standard C mount X 1 Image size 10x7mm lt 405 647nm 50mW gt after FC connector Laser safety Class 3B EX excitation Filter 1 Standard None 2 EX Filter Wh
46. ective earth terminal to avoid risk of electrification 35 Control Unit 1 RS232C cable connect with RS232C2 of control unit CSU X1CU F1 A CAUTION Please ONLY use supplied cables otherwise it is possible to fail EMC conformity 36 3 3 4 Connection Diagram CSU X1 A1 Camera PC INTERLOCK KEY RS23201 CSU X1 A2 MOTOR2 RS232C2 CSUX1CU F1 MOTOR PC INTERLOCK KEY R5232C1 37 CSU X1 A3 RS232C1 CSUX1CU B1 BORLA Camera Microscope MOTOR2 CSUX1CU F1 INTERLOCK KEY R323201 RSZIZEZ MN CAUTION You have to use supplied cables ONLY otherwise it is possible to fail EMC conformity 38 PP 3 3 5 Installation of Camera Please follow the user s manual of your camera when you mount it on the CSU X1 using the C mount adapter Insert the C mount adapter fixed to the camera into the camera port adapter on the unit Use the M3 screws to fix the camera adapter with the Allen wrench provided MN CAUTION The body of the CSU X1 is durable up to 5 kg If you install a camera heavier than 5 kg on the CSU X1 please use an appropriate support to fix the camera so that the CSU X1 will not be deformed When you install the CSU X1 to the side port of an inverted microscope and mount a camera horizontally please support the camera to prevent deformation of the camera adapter When you install the system onto an upri
47. eel CSU X1FW 06P 02 Option Please inquire EX filter wheel is not directly attachable to the CSU X1 body EX filter line up is shown in Table 7 1 1 3 Manual exchange Option 3 position slider is available please inquire EX filter line up is shown in the Tables 7 1 1 1 amp 25 DM dichroic mirror 1 Standard None 2 Option Size 13mmXx15mmX0 5mm DM line up is shown in the Table 7 2 2 DM exchange 1 Basic model with amp w o control unit Only CH1 in the DM block is valid DM block can be exchanged manually 2 High end model with control unit Three channels are available in a DM block DM block can be exchanged manually CAUTION Please ask the advice from your support service for safely exchanging the dichroic mirror block Please comply with the laser Class 3B safety precautions Laser input Yokogawa standard fiber supplied with the CSU X1 head standard fiber supplied with the CSU X1 head degrees ped AFC connector and overhead of the firmware 50 2 Life on off total count 60 000 lt 3 Continuous open shut frequency 3Hz gt BA barrier filter BA filter position and exchange Bright Field Non confocal Confocal switching Microscope connection Switching between 1st and 2nd camera port Camera port 1 Standard None 2 Option BA line up is shown in the Table 7 2 2 CAUTION You must install optimal BA filter to match the laser and DM 1 Basic model One
48. em and Adjustment of Fiber Couplev 31 3 3 3 Installation of CSU X1 seresssnvornssnavsrnssnnvnnnssnnvnvnnssnnvnnnssnnnsnnssnnnsnnssnnnnvnnssnnnnnnssne 32 3 3 4 Connection Diagram rronnnorvrnrrrseennennnnrnnnsrnseenennnnrsenssrsnensnnrnnsenssrssennnnnrnnsensseneene 37 3 3 5 Tnstallation of Camera iti acacia A hindi iain teint 39 3 3 6 PO Wer WIECH TT 39 3 3 7 Power Oli a it daa 40 4 Name and Function AA ARA ai AAA buede 41 41 Mi Model iia ceeds tated lances e e ee a ise Al 4 2 M2 Model css eccesdtavesvsssavc iia dada iii 44 43 PVT Mode Lia 3 cas sacha vesatet veh aS aa cad 44 44 A2 WOE O A A 45 45 23 Mode O 45 5 Maintenance dias ALTA A ERA EAT AE AA ATA 46 bl BA Filter Exchanges at ia oats AARTE 46 5 2 Dichroic Mirror Unit Exchange cccccccccccssssssseeeeecceceeansseeeeeeeceeeeaees 47 6 Trouble SOON E AAA A A ane ae aa 48 6 1 Microscope Laser and fiber coupler ooooccccccnnnnnnnnnnnnnnnnnnnnnnnononnnnnos 48 62 CUA at did 48 Zo SS DOCTHCAION lia ASIA AE AAA A AO 50 TL Product Specifications dia 50 7 1 1 Specifications of CSU X1 unit ococcnnnnnononanononncnnnnnanononononnononn nono noncnnonannnano na noncnnono 50 7 1 2 Electrical Specifications of CSU X1 Umit coccnnnnnuooononanonononananonononcnnononnanonannnnonnono 53 7 1 3 Specifications of Control unit occcnnnnnoouonnnonononnnanononnnnonnnnonnnnonononoonononnn nono nnnncnnono 54 7 1 4 Specifications of Filt
49. ems 6 1 Microscope Laser and fiber coupler Please cope with any problems specific in the microscope lasers camera or fiber couplers by following instruction manuals of each instrument 6 2 CSU X1 Problem Possible Cause Solution a No images Light path switching Push the knob and switch to 1st port confirmed knob is pulled when Motor not rotating Turn on the key switch Green LED microscope light path is at the camera Too weak To increase illumination light power port illumination of microscope b No confocal images confirmed No laser beam incident from the objective lens Turn on key switch Green LED Switch microscope light path to camera port Select through no DM block in the microscope Remove DIC polarizer Turn on the laser system Light path is at 2nd port Push the knob and switch to 1st port Laser is too weak for proper excitation Power up the laser Microscope illumination is ON Turn down the illumination Camera is not Inspect the camera following its instruction manual working c Visible dust Dust at the Clean cover slide glasses specimen Objective lens is Clean the objective lens following microscope dirty instruction Dust on the camera port DM or pinhole disk surface Remove dusts with air blow from the camera port aperture of CSU Some dust could not be removable with air blow Some dusts could be hard to remove But pleas
50. en you use control unit before shut down the CSU X1 by turning the key switch to OFF position MN WARNING e Ifyou shut down the CSU X1 when shutter is open shutter may not be completely closed In such case please switch on again so that the system returns to default status and shutter closes 2 Control Unit Please switch off the power switch at the back panel of the control unit 40 4 Name and Function 4 1 M1 Model 5 a 1 Key switch Turn from OFF to ON position when use at user initialized status explained later If you wish to re set the CSU X1 turn the key to OFF position and ON again You cannot remove the key switch at ON position 2 Power ON indicator Green LED lights when power is ON LED is off when power is OFF 3 Power port DC POWER input port 4 Interlock key port Insert supplied CSU X1 interlock key here for manual shutter control Remote shutter control is enabled by connecting with a control unit with interlock cable option For urgent system shut down it is possible to shut off laser beam and stop disk motor by disconnecting the interlock key 5 Shutter button Momentary switch to open close shutter 6 Shutter light Red LED lights when shutter is open no light when closed 41 7 FC connector FC connector of the fiber is connected here Please firmly connect the fiber by adjusting to the guide key and tightly screw Please use supplied FC connector cover for
51. er Wheel cccccccccccccecesssssscececececsesssseceeeseesesenssseceeeeeess 55 7 1 5 Enviroment oea A aksene aes 56 7 1 6 CONAM A RER 57 7 1 7 Global Environment Action r rrrrrroornnnnrrrnrrrsrensnnrnrrnnnsnrsrenennnnrnnnssrssensnnrnnnsenenne 57 7 2 Model and Suffix Code MS Code 0 ccesssssssceseseseseseseseseseseeeeeeeeeeeeesenes 58 7 3 DIMENSION as 60 7 3 1 CSU XTA TA knee See 60 7 3 2 CSW EXT AD rta 61 7 3 8 CSUX TAS sne nn 62 7 3 4 CSUXEM LET 63 7 3 5 CSU Madden a dana ee o Da oca 64 7 3 6 ESD ED C K E O ANA enl 65 7 3 7 CSUATEWS TET SS 65 TA Controla ars 66 Bi Command DIS a AN aa 73 DS IVETTE RAGDE 75 1 Safety Precautions 1 1 Introduction Thank you so much for purchasing the CSU X1 This manual covers the features operation methods and procedures safety and handling precautions of the CSU X1 Before you start to use this instrument read this manual thoroughly and operate the instrument in a proper manner After reading through please keep this manual in a safe place for future quick reference 1 2 CAUTION No part of the user s manuals may be transferred or reproduced without prior written consent from Yokogawa Yokogawa continues to improve the product s performance and features and for this reason the specifications and information herein are subject to change at any time and without notice The authors and publishers of this manual have used their best efforts in preparing this document
52. er safety labels Interlock key for CSU X1 unit Hexagonal wrench set Dust cover at laser path 2nd camera port cover Camera port support for inverted microscope installation Support screws CSU key switch Standard fiber Table 7 1 1 1 EX filter list for EX Filter Wheel 635 648 HQ650 45x Table 7 1 1 2 EX filter list for manual exchange Ex Asahi g20 TXC117 TXC118 TXC119 52 7 1 2 Electrical Specifications of CSU X1 unit Specification External 1 Basic model Control Low speed No control unit is necessary Standard Requires CSU X1CU F1 control unit and a PC High speed Requires CSU X1CU F1 control unit and a PC 2 High end model Requires CSU X1CU F1 control unit and a PC Interlock key l Installed for CSU X1 Manual shutter switch becomes valid unit Basic 2 Uninstalled model Shutter closes motor stops and manual shutter switch becomes invalid Interlock key l Installed for control unit Manual shutter switch becomes valid External shutter control from PC High end through control unit becomes possible model 2 Uninstalled Shutter closes motor stops and manual shutter switch becomes invalid DM exchange DM exchange should be conducted after switching off CAUTION Disks could be fatally damaged if DM block touches the rotating disks Tf DM holder case were opened when CSU is working interlock works to lose shutter and stop disk rotation Rotation speed 1 Basic model w o c
53. ever touch the tip of the fiber and be most careful to keep the fiber tip clean If you touch or damage the tip the laser output power at the fiber will be affected and thus the confocal image will be impaired When you measure the laser output power at the fiber tip please set the laser power at the minimum level Possibility of damage to the fiber due to air dust would be higher if the output is set higher and the laser beam might be diffused To prevent damages on the system please use the laser of less than 100mW Use of higher than 100mW laser may damage the CSU X1 system Use of control adjustment or performance of procedures other than those specified herein may result in hazardous radiation exposure Never use lasers outside of the specification NOTE Recommended laser specification Range of wavelength 405nm to 647nm Laser power 100mW of less at either input to the CSU X1 or fiber output NOTE The best efficiency of laser coupling by the coupler is 50 or more To examine laser outputs from your laser system and the fiber it is recommended to use a power meter for measurement 31 3 3 3 Installation of CSU X1 IN CAUTION Do not turn on the laser system before all the installation and connection procedures described below have been completed 1 Mechanical and optical installation and connection Attach the microscope C mount adapter to the bottom of the CSU X1 by turning clockwise until ha
54. ght type microscope and total weight of confocal system including CSU X1 its accessories including a camera surpassed microscope s maximum weight tolerance it is necessary to support total system Please inquire your local sales representative 3 3 6 Power Switch e Control Unit Please confirm if the control unit Interlock key is inserted into EXT INTERFACE1 1 LOCK at the backside of the control unit If not please insert supplied key Also please confirm if CSU X1 and the control unit is connected with supplied cable And then put the power of the control unit ON e CSU XI 1 Model without a control unit Please insert CSU X1 Interlock key into EXT CONTROL INTERLOCK connector before put ON the power The interlock is unlocked enabling you to manually open close the shutter from the operation panel 2 Model with a control unit CSU X1CU F1 Shutter control via panel switch or control unit becomes possible after CSU X1 power is ON 39 A WARNING Never insert your fingers hand into the holes in the filter wheel If you do while the wheel is moving you may injure your fingers hand MN CAUTION Please refer to the specification sheet in this manual to learn the conditions of filter wheel moving speed If you try to move too fast the filter wheel could fail to move properly 3 3 7 Power Off 1 CSU X1 Please close the shutter first either manually by shutter button or by shutter close command wh
55. ich is an emission light path to bypass the disks in the CSU X1 and allows you to use the same camera for both confocal and non confocal imaging without light loss Light path switching is controlled by the control unit CSU X1CU F1 Bright Field means non confocal wide field images such as DLC phase epifluorescence and TIRF 24 2 3 5 CSU X1 A3 AAPOR eS G e CSUX1CU B1 ot ED2pHXIN po d bunny Microscope Camera LESUX1CU F1 Laser inlet FC connector shutter Excitation filter Option Dichroic mirror See Specification List Barrier filter See Specification List Camera port 8X8mm adapter for EMCCD is available Option H Bright Field Unit Dichroic mirror block automatic control for up to three dichroic mirrors Filter Wheel for up to six barrier filters Automatic Light Path Switching port This model is CSU X1 A1 plus 24 camera port to enable installation of two cameras Light path switching between two camera ports is controlled by the control unit CSU X1CU F1 Dichroic mirror for simultaneous imaging with two cameras can be installed It is also possible to install confocal ocular port instead of the second camera 25 3 Before Using the Unit 3 1 Packing List Part name Part number QTY Description CSU X1 CSU X1 1 AC adapter M3916UD 1 Power supply for CSU X1 Power cable A1077WD 1 AC adapter cable i
56. if overloaded and becomes over current status As shown below reset button gets out and white indicator shows up White indicator S 20 3 Resetting of circuit breaker You can reset the circuit breaker by pushing the button one minute after shut down However if over current status still continues top free function works and you cannot reset AN Caution In case circuit breaker worked to shut down there should be a problem in the circuit of this unit If you cannot reset please consult with your local representative Do not repeat resetting many times 6 Specification 6 1 Product Specification 6 1 1 Control Unit Rotation speed Standard 1500 5000rpm Max 1000fps control High Speed 1500 10000rpm Max 2000fps Possible for command control of rotation speed setting Rotation speed setting reading resolution 1500 5000rpm 1rpm 5000 10000rpm 2rpm External sync is possible by pulse signal input via external signal input terminal External output terminal can send 12 pulse per one rotation DM Exchange Command control of exchanging up to 3 DM positions in the DM block External I O Digital I O TTL Input 4CH Contact Input 4CH Open control Collector output 4CH Contact output 4CH External shutter control synchronization control of CSU rotation speed synchronization signal output etc Analogue input 8 ports O 5V 8Bit 0 255 Response 100ms Analogue output 4 ports O 5V 8Bit 0 255
57. in 8 8 Short circuit with pin 7 9 NC Unconnected Table 7 4 5 RS232C2 D Sub9 pin connector for control unit Pin No Signal Name Function I O Connection 1 NC Unconnected 2 RXD Input RS232C compliant 3 TXD Output RS232C compliant 4 NC Unconnected 5 GND 6 NC Unconnected 7 Short circuit with pin 8 8 Short circuit with pin 7 9 NC Unconnected Table 7 4 6 MOTOR1 D Sub15 pin connector for control unit 1 CSU X1CU F1 Pin No Function I O Connection 1 MOTORI1 output Output 2 MOTOR1 output Output 3 MOTOR1 output Output 4 MOTOR1 output Output 5 MOTOR1 output Output 6 MOTOR1 output Output 7 MOTOR1 output Output 8 MOTOR1 output Output 9 Unconnected 10 Unconnected 11 Sencer input Input 12 Unconnected 13 Unconnected 14 Source for sensor 24V 15 GND for sensor 70 Table 7 47 MOTOR2 D Sub15 pin connector for control unit 1 CSU X1CU F1 Pin No Function I O Connection 1 MOTOR2 output Output 2 MOTOR2 output Output 3 MOTOR2 output Output 4 MOTOR2 output Output 5 MOTOR2 output Output 6 MOTOR2 output Output 7 MOTOR2 output Output 8 MOTOR2 output Output 9 Unconnected 10 Unconnected 11 Sensor input Input 12 Unconnected 13 Unconnected 14 Source for sensor 24V 15 GND for sensor Table 7 48 MOTOR1 D Sub15 pin connector for control unit 2 CSU X1CU
58. inal Acquire input terminal level EI ch i purpose Set DA output parameter DA_DATA ch n Ve Purpose Set DA output parameter DA DATA ch n er eee Acquire AD input value AD_DATA ch Memory control Save memory Save parameters SAVE_MEM Save Filter Wheel information FW INFO Ich n XXX max 16 letters Xx Save dichroic mirror information max 16 letters DM INFO n XXXX Memory load Load parameters from memory L LOAD MEM Load default parameter LOAD INIT 34 Acquire Filter Wheel information FW INFO ch n Acquire dichroic mirror information DM INFO ch Hardware CSU Information Acquire serial number of CSU information SERIAL_CSU Acquire product ID of CSU PRODUCT_CSU Error information Acquire error 1D ERRID Connection status Acquire hardware connections SYSTEM Software ee Acquire software version VER information Acquire build time of software BULIDTIME Hardware misc Output error ID log LOG ON Not to output error ID log LOG OFF Acquire connection status of own control unit SYSTEM THIS Acquire connection status of extension control unit SYSTEM EXT Command notation colon or space delimiter ch channel number of connected instruments n integral number parameter f decimal number parameter
59. ions to attach warning labels to CSU X1 A3 E 15 CSU X1 FW CSU X1 M1 CSU X1 A2 A CSU X1 A3 Figure 1 6 Positions to attach warning labels 6 16 Figure 1 7 Examples of warning labels attached to the microscope 17 2 Product Overview 2 1 Principle of the Confocal Microscope As conventional optical microscopes use surface light sources such as halogen lamp or others diffused light from places other than the point of interest are mixed with the light from the point of interest Further when planar photo detectors like cameras or eyes are used blurred out of focus light from the points shifted toward the optical axis are observed overlapped with the observed light For these reasons the conventional optical microscope has a limited spatial resolution On the contrary confocal microscope systems use laser beams to illuminate samples with pin point accuracy to eliminate diffused light from sources other than the point of interest In addition pin hole windows are provided in front of the light detector to cut off the light from sources other than the point of interest for high resolution Detector Light source Y Beam splitter Pinhole window Objective lens Focal plane Specimen Figure 2 1 Principle of the confocal microscope 18 2 2 Structure and Features of the CSU X1 The CSU X1 has a combination of upper and lower disks rotated by a motor As shown in Figure 2 2 ab
60. ir instructions To avoid damage to the CSUX1FW make sure that input and output terminals satisfy the specifications The CSUX1FW is high precision optical product Do not install or use the CSUX1FW in areas with excessive vibration excessive or conductive dust high humidity excessive heat near heat sources or in direct sunlight sudden temperature change that may cause condensation corrosive or flammable gas etc Do not touch any parts inside the CSUX1FW Any dust soil or minor damage on the optical parts inside the CSUX1FW may result in damages In case of a mechanical trouble in the CSUX1FW never touch inside the instrument and contact Yokogawa Electric Corporation or its representative 2 Product Overview 2 1 Product overview The CSUX1FW is a high speed filter wheel to be used in combination with the confocal scanner model CSU X1 The Control unit CSUX1CU FI optional is necessary to use the CSUX1FW One CSU control unit CSUX1CU F1 can control up to two units of CSUX1FW Two units of CSUX1FW mounted to CSUX1 A3 Second Camera Model 3 Before Using the Unit 3 1 Packing List Filter Wheel CSUX1FW Supplied with CSUX1 A1 A2 A3 or CSUX1 M1N M1H M2N M2H Part name Part number QTY Description Filter wheel M3918MA 1 Filter wheel Aluminum disc for M3916MW 6 Aluminum disc to fill open positions in the weight balance filter wheel for weight balance Connection cable 1 M3918AP 1 Connecti
61. irement MN CAUTION 1 Inspection of tripod of the microscope Laser beam reflected at the surface of specimen cover glass could possibly return the light path emerge at the microscope eyepiece and be directed into your eyes To prevent a hazardous accident please inspect your microscope and take the necessary measures in accordance with the following directions Please only use 0 100 100 0 switch to select the light path between camera port and eyepiece port of the microscope Before installing the CSU X1 to the microscope please inspect the microscope If there are reflective surfaces inside the microscope due to reasons such as damage to inner painted areas in the microscope or damaged prism masking laser beam could be reflected and directed into the microscope eyepiece Please repair any damage inside the microscope before installing the scanner unit 2 Modification of non 100 0 microscope Some microscopes typically Olympus BX50 series have a slider switching between eyepiece and camera port other than 100 0 or 0 100 such as 20 80 If the switching slider was set at a non 100 0 position part of the reflected laser beam will reach into the microscope eyepiece Never use such non 100 0 position for your own safety It is highly recommended to seal such non 100 0 slider windows with black tape so as to prevent accidental laser exposure Figure 3 2 shows examples of where to seal the 20 80 windows of the switching slide of the
62. is invalid Protective earth Avoid electrical shock ensure the protective earth before turning on the power The power supply code attached the CSUX1FW is tripolar power strip with earthing conductor thus please use tripolar power receptacle with protective earth terminal If you use an adapter that exchangeable between tripolar and bipolar ensure the protective earth terminal connected with earthing conductor of the conversion adapter Necessity of the protective earth Avoid mechanical failure never cut off internal and external of protective earth conductor and unfix the wire connection of the protective earth terminal Defection of protective function Never apply the CSUX1FW when defection of protective function such as protective earth and fuse appear Ensure the protective function before using the CSUX1FW e Use under gas It is extremely dangerous to use this instrument under the atmosphere with explosive or evaporating gas or vapor e Disassemble the case There are high voltage place internal of the CSUX1FW never disassemble the case except the service personnel External connection Ensure the protective earth before connect with referent hardware A CAUTION The CSUX1FW is used in combination with CSU X1 confocal scanner unit CSU control unit laser system microscope and cameras to be purchased separately In addition to this manual read carefully the user s manual of the respective products and follow the
63. is symbol indicates that the operator must refer to an explanation in the user s manual in order to avoid serious injury or death and or damage to the instrument A CAUTION This symbol indicates that the operator must refer to an explanation in the user s manual in order to avoid minor injury and or damage to the instrument To reduce the risk of injury of personnel such as electric shock or and or damage to the instrument please comply the following precautions MN WARNING Power supply Ensure the source voltage matches the voltage of the power supply before turning on the power Please only use supplied power cable Power strip and plug To avoid electric shock or fire please only use power code supplied by YOKOGAWA Be sure to connect main power supply plug with grounded power receptacle If you use extension code without protective earth terminal protected operation becomes invalid Protective earth To avoid electrical shock ensure the protective earth before turning on the power The power supply code of CSUX1CU is a tripolar power strip with earthing conductor thus please use tripolar power receptacle with protective earth terminal Necessity of the protective earth Do not cut internal or external protective earth conductor or unfix the wire connection of the protective earth terminal otherwise this instrument may get in danger Defection of protective function Never operate this instrument if protective function
64. ive in such unusual case Do not put any objects on the cable Do not let the cable touch heating objects When you disconnect the cable do not pull the cable but always pull the connector In case a cable was damaged please contact your local representative for replacement order You may find necessary parts number in the packing list of this manual 3 2 2 General precautions on handling Please do not place any liquid containing objects on this unit since liquid spilling could damage the unit Be careful not to add mechanical shock or drop the unit which could result in severe damage Application of mechanical shock to the cable could cause electric noise which may lead to malfunction Avoid bringing any charged object close to the terminal which may cause failure Please be most careful when you carry this unit Remove the connecting cable first then use the handle on this unit to carry This unit weighs about 5 kg Please do not wipe the plastic surface with organic solvents such as benzene or paint thinner which may cause discoloration When the surface of the unit gets dirty please clean with soft cloth wet with dilute solvent and then wipe with a dry cloth 10 3 3 Installation 3 3 1 Installation requirements Please use the FW under the following environment Ambient air temperature 15 40 Ambient humidity 20 75 RH No dew condensation MN CAUTION e Dew formation inside the unit may occur when you move
65. jured when the filter wheel moves A CAUTION Please fill all empty ports with supplied aluminum discs one disc at one port for weight balance Otherwise filter wheel may fail normal function Aluminum disc 18 6 Specification 6 1 Specification 6 1 1 Specification of filter wheel Installable Size p25mm gt filters Max 6 filters It is necessary to fill all open positions with dummy discs to keep weight balance Exchange 33msec to the adjacent position It is necessary to wait for speed 33msec lt before moving to the next position 66msec to move two positions It is necessary to wait for 33msec lt before moving to the next position 99msec to move three positions It is necessary to wait for 33msec lt before moving to the next position Control unit makes the filter wheel move toward shortest direction Accessories 6 Aluminum disks dummy filter to balance weight Special tool to screw unscrew filter fixing rings Connection cable Power supply 24V DC 3 74 Supplied from the control unit 6 1 2 Environment Specification Environment 1 Operation temperature and relative humidity range 15 40 C 20 75 RH No condensation 2 General Environment 20 70C 5 95 RH No condensation 1 112 W X 100 H X 226 L excluding edged attachments 1 9Kg 1 Desirable environment for delivery storage or whatever when the instrument is not in operation under which the instrument may not suffer unre
66. lens system allows about twice highly efficient excitation power 1 b High speed shutter to work minimizing laser damage photo bleaching c Doubled the maximum scanning speed from 1 000fps CSU22 to 2000fps High speed model d Bright Field Switching port CSU X1 A2 M2 is an emission light path to bypass the disks in the CSU X1 and allows you to use the same camera for both confocal and non confocal 2 imaging without light loss e Second camera port CSU X1 A3 is for either to install a second camera or direct view ocular port 3 By using an appropriate dichroic mirror simultaneous two color imaging with two cameras is possible f User can manually exchange the dichroic mirror blocks in the CSU X1 g High end models CSU X1 A1 A2 A3 provide controllable dichroic mirror block for up to three dichroic mirrors h Basic model provides a dichroic mirror block for one dichroic mirror i High end models CSU X1 A1 A2 A3 provide function to control Yokogawa high speed filter wheel j Direct C mount adapter for 8X8 mm CCD camera such as EMCCD camera is available 1 Actual light efficiency depends on the total system including the microscope optics 2 Bright Field means non confocal wide field images such as DIC phase epifluorescence and TIRF 3 It is necessary to use appropriate barrier filter for laser safety 20 2 3 1 CSU X1 M1 F Camera D mmm Control unit CSU X1CF F1 is
67. nd tight in accordance with the microscope user s manual Be careful not to thread too tight When you set the CSU X1 to side port of an invert type microscope please support the CSU X1 with the level screw provided so that the adapter will not be deformed Depending of the microscope type it may be necessary to insert appropriate spacers under the microscope 2 Electrical connection Plug the DC plug of the AC adapter into the DC POWER of the CSU X1 You must always use the AC adapter supplied with CSU X1 For laser safety the scanner power switch is designed as a key switch To turn the unit on insert the key provided 3 Connection of fiber Connect the fiber to the FC connector port of the CSU X1 by matching the guide key on the FC connector and fiber connector and turning the screw tightly Attach fiber guard N CAUTION Please only use CSU X1 specific optical fiber supplied with the CSU X1 head for the best optical performance In case different optical fibers were used CSU X1 may not be able to work at its best optical performance 4 Interlock If you use the optional control unit please refer 5 and 6 Before use insert CSU X1 interlock key supplied with the unit into the interlock connector and then turn the power switch on The interlock is unlocked enabling you to manually open close the shutter from the operation panel 32 1 Connect AC adapter Pa 4 Connect either interlock key or CSU co
68. nected 11 Sencer input Input 12 Unconnected 13 Unconnected 14 Source for sensor 24V 15 GND for sensor 31 Table 6 4 7 MOTOR2 D Sub15 pin connector of control unit 1 CSUX1CU F1 Pin No Function I O Connection 1 MOTOR2 output Output 2 MOTOR2 output Output 3 MOTOR2 output Output 4 MOTOR2 output Output 5 MOTOR2 output Output 6 MOTOR2 output Output 7 MOTOR2 output Output 8 MOTOR2 output Output 9 Unconnected 10 Unconnected 11 Sensor input Input 12 Unconnected 13 Unconnected 14 Source for sensor 24V 15 GND for sensor Table 6 4 8 MOTOR1 D Sub15 pin connector of control unit 2 CSUX1CU B1 Pin No Function T O Connection 1 Unconnected 2 Unconnected 3 Unconnected 4 Unconnected 5 Unconnected 6 Unconnected 7 Unconnected 8 MOTOR output Output 9 MOTOR output Output 10 MOTOR output Output 11 Sensor input Input 12 MOTOR output Output 13 MOTOR output Output 14 Source for sensor 15 GND for sensor 32 Table 6 4 9 MOTOR2 D Sub15 pin connector of control unit 2 CSUX1CU B1 Pin No Function T O Connection 1 Unconnected 2 Unconnected 3 Unconnected 4 Unconnected 5 Unconnected 6 Unconnected T Unconnected 8 MOTOR output Output 9 MOTOR output Output 10 MOTOR output Output 11 Sensor input Input 12 MOTOR output Outpu
69. ner not specified in this manual the protection provided by this instrument may be impaired In addition Yokogawa Electric Corporation assumes no liability for the customer s failure to comply with these requirements The following symbols are used on the instrument Mi warkine or AN CAUTION Both of the above two symbols indicate that important content is mentioned The symbols are given wherever you are required to refer to the manual in order to avoid personal injury and or damage to the instrument The following symbols are used in this manual MN WARNING This symbol indicates that the operator must refer to an explanation in the user s manual in order to avoid injury or death of personnel and or damage to the instrument MN CAUTION The symbol indicates that the operator must refer to an explanation in the user s manual in order to avoid minor injury and or damage to the instrument To reduce the risk of bodily injury or of personnel and or damage to the instrument observe the following precautions MN WARNING e Power supply Ensure the source voltage matches the voltage of the power supply before turning on the power Power strip and plug Avoid electric shock and fire disaster please use a power strip that provided by YOKOGAWA Connect the main power supply plug with power receptacle that comprise a protective earth terminal If you use extension code without protective earth terminal protected operation
70. ng on CSU X1 Models Dichroic mirror movement High end Models CSU Shutter ON OFF Command level or external control External Input Output signal control Filter Wheel CSUX1FW movement CSUX1CU F1 type Bright Field port switching CSUX1CU Bitype Interlock Communication RS232C Fig 2 1 CSUX1 A2Model CSUX1CU Fland CSUX1CU B1 3 Before Use 3 1 Packing List 3 1 1 CSU Filter Wheel Control Unit CSUX1CU F1 Name Parts No QTY Usage Filter Wheel Control unit M3918EA 1 To control FW Source cable A1006WD 1 UL CSA standard Controller Interlock cable M3918AN 1 Interlock cable for CSU control 2 5m shield cable Communication cable M3918WA I Serial cross cable DSUB 9pin female female 3m shield cable Controller Interlock key M3918AT 1 Interlock key for CSU control unit 3 1 2 CSU Bright Field Control Unit CSUX1CU B1 Name Parts No QTY Usage Bright Field Control Unit M3918EB 1 To control Bright Field Source cable A1006WD 1 UL CSA standard Bright Field1 Cable M3918AR I Bright Field1connection cable 2 5m shield cable Bright Field2 Cable M3918AS 1 Bright Field2connection cable 2 5m shield cable CSU Control unit cable 1 M3918AN 1 Interlock cable to control CSU 2 5m shield cable Communication cable M3918WA I Serial cross cable DSUB 9pin female female 3m shield cable Controller Interlock key M3918AT I Interlock key for
71. nnecting cable 3 Connect CSU X1 specific fiber The photo shows the CSU X1 M1 model but the same for M2 Al A2 and A3 models 5 Connection with control unit CSU X1CU F1 a Connect the CSU connector of control unit CSU X1CU F1 and the EXT CONTROL INTERLOCK connector of CSU X1 with supplied cable b Insert controller interlock key to EXT CONTROL INTERLOCK c Connect RS232C1 connector of the control unit and a PC with RS232C cable d Connect MOTOR 1 connector and Filter Wheel 1 with supplied FW1 cable e Connect MOTOR2 connector and Filter Wheel 2 with supplied FW2 cable if necessary f Connect power cable after confirming the power switch is OFF Please use power receptacle with the following specification 3 prong plug with protective earth terminal Rated supply voltage 100 240VAC Voltage frequency range 90 264VAC Rated supply frequency 50 60Hz Supply frequency tolerance 48 63Hz Max power consumption 200VA MN WARNING e Please confirm your power supply is suitable for the rated supply voltage of the CSU X1 before connection 33 Please confirm all main switches of CSU X1 and attached instruments are OFF before connecting power cord Please only use provided power cord to avoid any risks such as fire or electrification Please always use 3 prong plug with protective earth terminal to avoid risk of electrification CSU control cable ferrite core at this side
72. not touch or look into the laser beam 6 Warning label 6 Warning sign to indicate the operator must never insert the fingers or hand in the filter wheel holes while operation 7 Laser key switch The laser power supply used for CSU X1 system employs a key switch for your safety The key switch is to turn off laser emission into the CSU X1 For details see the user s manual of the laser system you are using 8 Shutter The shutter is for the incident laser beam You may select full open or close For non confocal observation always keep the shutter closed Red LED light on the operation panel of CSU X1 will be OFF when the shutter is closed 9 Interlock When you use the CSU X1 without control unit CSU X1CU F1 insert the interlock key into the EXT CONTROL INTERLOCK connector before turning on the unit This will disengage the interlock circuit and you will be able to manually open close the shutter from the operation panel If you use the control unit CSU X1CU F1 insert the interlock key into the EXT INTERFACE at the back of the control unit After turn on the CSU X1 unit you will be able to open close the shutter via operation panel or control unit 10 1 5 2 About the Class 3B Laser Laser products are classified by their acceptable exposure limit AEL based on the laser beam wavelengths and power characteristics and a common set of safety standards is applied to the products in each class The CSU X1 is classifie
73. nspection and modification described in Items 1 and 2 on the next page and operate the microscope and unit by following the guidelines given below In confocal observation the light observed through the eyepiece of CSU X1 Option is weak fluorescence from the specimen The unit is designed in a way that no reflected laser beam should be observed via the eyepiece if proper barrier filter is installed However when the system is under automatic control there is a possibility of leakage of laser beam into the eyepiece which is extremely hazardous If you control the filters from the computer commands DO NOT LOOK INTO THE EYEPIECE NOTE When incident laser power 488 nm is 30mW at the end of the fiber incident laser power at the objective lens is about 3mW typical Assuming the multiple reflection rate of the cover glass to be 8 the reflected laser beam could be around 0 24mW which corresponds to Class II of JIS laser safety standard 488nm 1mW or less at CW Brief summary of laser safety standard Class 1 Intrinsically safe having little possibility of damaging eyes if looked at for some time Class 2 Little possibility of damaging eyes if the exposure time is less than 0 25 seconds Class 3B Hazardous to eyes if the laser beam is looked at directly Even if you use a laser beam weaker than this class damage to the eyes may occur MN CAUTION Please refer to laser safety manual to fully understand laser safety requ
74. o so shutter closing timing could delay and laser beam may leak out Table 7 4 3 PCR36 pin male connector for control unit Pin No Signal Name Function I O Connection 1 AD1 1 Analogue input 1CH Input Resolution 0 5V 10bit 2 AD2 1 Analogue input 2CH Input Precision 8bit 3 AD3 1 Analogue input 3CH Input ps 4 AD4 1 Analogue input 4CH Input 5 AD5 1 Analogue input 5CH Input p m Ios 6 AD6 1 Analogue input 6CH Input 22 T AD7 1 Analogue input 7CH Input ee 8 AD8 1 Analogue input 8CH Input Noa Maa 9 DA1 2 Analogue output 1CH Output Resolution 0 5V 12bit 10 DA2 2 Analogue output 2CH Output Precision 8bit 11 DA3 2 Analogue output 3CH Output git 12 DA4 2 Analogue output 4CH Output 4 a 1 i 1f DA j 50 13 NC Unconnected 14 NC Unconnected 15 NC Unconnected 16 NC Unconnected 17 NC Unconnected 18 Reserved 19 30 GNDA _ Analogue GND 31 35 NC Unconnected 36 GNDA _ Analogue GND 1 Possible to acquire AD ch1 ch8 data AD_DATA ch 2 Possible to assign AD ch1 ch8 output value DA DATA ch n 69 Table 7 44 RS232C1 D Sub9 pin connector for control unit Pin No Signal Name Function I O Connection 1 NC Unconnected 2 RXD Input RS232C compliant 3 TXD Output RS232C compliant 4 NC Unconnected 5 GND 6 NC Unconnected 7 Short circuit with p
75. observe the explanation in the user s manual in order to avoid minor injury and or damage to the instrument To reduce the risk of bodily injury of personnel such as electric shock or and or damage to the instrument please comply the following precautions MN WARNING Power supply Please make sure the source voltage matches the voltage of the power supply before turning on the power The CSU X1 is classified as a laser hazard CLASS 3B instrument in accordance with WHO regulations Please comply with the remarks on laser safety precautions described in the next chapter of this manual MN CAUTION The CSU X1 is used in combination with a microscope laser system camera and other peripheral instruments Please read carefully instruction manuals of each instrument and follow safety precautions Please use input output terminals of the CSU X1 within the range of specification as stated in this manual The CSU X1 is a precision optical instrument Please do not install the CSU X1 system under inappropriate circumstances such as vibrating conditions dusty place very humid and or high temperature room drastic change in the room temperature corrosive or inflammable atmosphere etc Do not touch inside the CSU X1 Unauthorized open touch inside the CSU X1 may cause mechanical and optical damages and may cause serious damages Do not touch and soil the surface of the lenses inside the camera port adapter and C mount port of the CSU X
76. on cable for filter wheel 1 1 2 5m shield cable Connection cable 2 M3918AQ 1 Connection cable for filter wheel 2 1 2 5m shield cable Filter Wrench M3916CX 1 Tool for exchanging filters in the filter wheel 1 Connection cable 1 is supplied with CSUX1FW 06P 01 and Connection cable 2 1s supplied with CSUX1FW 06P 02 A CAUTION e You have to use attached cables ONLY otherwise it is possible to fail EMC conformity 3 2 Before Use 3 2 1 Safety Precautions e Before you start to use the unit please read this manual thoroughly e Do not remove the cover to avoid risk of burn injury since some parts inside the unit could become high temperature Please contact Yokogawa Electric Corporation or its representative when you need inspection or adjustment of the CSUX1FW e Incase of such abnormal status as unusual odor or smoke goes up from the CSUX1FW turn off the power switch of the controller and contact Yokogawa Electric Corporation or its representative e Do not put any objects on the cable Do not let the cable touch heating objects When you disconnect the cable do not pull the cable but always pull the connector In case a cable was damaged please contact your local representative for replacement order You may find necessary parts number in the packing list of this manual 3 2 2 General precautions on handling e Please do not place any liquid containing objects on this unit since liquid spilling could dam
77. ontrol unit Fixed at 1800rp Can synchronize with external pulse signal within1500 1800rpm range 2 Basic model and High end model with control unit External rotation speed control from PC is possible Can synchronize with external pulse signal within the designated range Shutter control l Manual shutter switch inside CSU Momentary switch 2 External control PC control through CSU X1 F1 control unit Max allowable switch on off is 3 times second 3 Hardware control Shutter hardware control is possible with TTL level signal input to the external input terminal of CSU X1 F1 control unit Maximum repeated shutter ON OFF count per second is recommended at 3 times sec More frequent ON OFF could damage shutter Shutter Red LED when open Power AC adapter gt consumption Input 100 240VAC 10 50 or 60Hz 38W gt Output 24VDC 1 6A gt CSU Unit gt Input 24VDC 1A gt 53 7 1 3 Specifications of Control unit Rotation speed Standard 1 500 5 000rpm Max 1 000fps control High Speed 1 500 10 000rpm Max 2 000fps Possible for command control of rotation speed setting Rotation speed setting reading resolution 1 500 5 000rpm 1rpm 5 000 10 000rpm 2rpm External sync is possible by pulse signal input via external signal input terminal External output terminal can send 12 pulse per one rotation External I O control Digital I O TTL Input 4CH Contact Input 4CH Open Collector output 4CH Contact outpu
78. out 20 000 microlens in the upper disk focus collimated light from a laser on corresponding pinholes in the lower disk which are arranged in the same pattern as the microlens on the upper disk The light passing through the pinhole is focused by an objective lens on the specimen Light from the specimen returns along the same path through the objective lens and pinholes is reflected by a dichroic mirror and is focused at a camera or eyepiece The upper disk containing the microlens is mechanically connected to the lower disk containing the pinholes and a motor rotates the both disks Thus the light beams can illuminate the entire observation area of the specimen and forms a confocal optical slice at the camera or eyepiece The CSU X1 has a built in microcomputer It is possible to control various operations by communication with an external computer optional Microlens Disk Array Camera Beam Splitter Pinhole Disk Array Objective Lens aim Q J Specimen Focal Plane Figure 2 2 Basic configuration of the CSU X1 2 3 Description of CSU X1 CSU X1 was developed as an advanced model of our conventional CSU10 22 models which have been widely used as a standard tool for live cell imaging As enlisted below CSU X1 is significantly improved in its basic optical features Moreover various optional systems are available to allow flexible design of total imaging system as you need a Newly developed
79. put 7 12 DA4 2 Analogue output 4CH Output i z a 1 1 DA i 50 A 13 NC Unconnected 14 NC Unconnected 15 NC Unconnected 16 NC Unconnected 17 NC Unconnected 18 Reserved 19 30 GNDA _ Analogue GND 31 35 NC Unconnected 36 GNDA _ Analogue GND 1 Possible to acquire AD ch1 ch8 data AD_DATA ch 2 Possible to assign AD ch1 ch8 output value DA DATA ch n 30 Table 6 4 4 RS232C1 D Sub9 pin connector Pin No Signal Name Function I O Connection 1 NC Unconnected 2 RXD Input RS232C compliant 3 TXD Output RS232C compliant 4 NC Unconnected 5 GND 6 NC Unconnected 7 Short circuit with pin 8 8 Short circuit with pin 7 9 NC Unconnected Table 6 4 5 RS232C2 D Sub9 pin connector Pin No Signal Name Function I O Connection 1 NC Unconnected 2 RXD Input RS232C compliant 3 TXD Output RS232C compliant 4 NC Unconnected 5 GND 6 NC Unconnected 7 Short circuit with pin 8 8 Short circuit with pin 7 9 NC Unconnected Table 6 4 6 MOTOR1 D Sub15 pin connector of control unit 1 CSUX1CU F1 Pin No Function I O Connection 1 MOTOR1 output Output 2 MOTOR1 output Output 3 MOTOR1 output Output 4 MOTOR1 output Output 5 MOTORI1 output Output 6 MOTOR1 output Output 7 MOTOR1 output Output 8 MOTOR1 output Output 9 Unconnected 10 Uncon
80. r product to be attached to this product and the microscope to be used in combination with it Figure 1 2 shows the positions to attach the warning labels for the CSU X1 and the filter wheel Figure 1 3 shows the positions to which the warning labels for the microscope to be used in combination with this instrument should be attached Warning label 1 M3916LK E Warning label 2 M3916LL E Warning label 3 M3916LM E Warning label 4 D8953RH E Warning label 5 M3916MB Warning label 6 M3916XE Figurel 1 Warning labels 1 Warning label 1 Warning sign to indicate this product belongs to the Class 3B laser product and indicates the product s wavelength maximum laser output power and laser class 2 Warning label 2 Warning sign to instruct the operator must turn the laser off when he removes the FC connector 3 Warning label 3 Warning sign to indicate the laser aperture must be closed when the laser is on 4 Warning label 4 For Direct view Port Option Warning sign to instruct the operator avoid looking into the eyepiece of microscope when exchanging the light path between confocal and non confocal observation to avoid laser exposure through the eyepiece 5 Warning label 5 Warning sign to advise the necessity to use the laser in compliance with the class 3B laser safety precautions Specifically it is necessary to avoid direct exposure of the laser beam on the skin or eyes Do
81. rkeste dvenedee 12 8 328 O NN 17 4 Name and Function ies ani sissieccheneancgevbeiees e a a a E 18 Maintenances ii daria a 20 Dil Crreuit Break A dass 20 Go IO PEC CAOS A EA A RO ES A 22 6 1 Product Specification e a aE EAA L Naaa A AAA ET AAA ii 22 6 1 1 Control UNTIL A ott esha besa thes case eck des 22 6 1 2 Filter Wheel Option srrnmrrrnnnnnnnnnvnnernvnnnnnnnnvvevernvnnsnnnnnenennnnnsssnnnnevesennnssssnnnnee 23 6 1 3 Environment itevsssveseeeecasatesgeatebestesseaes ssyseede T T A E 24 6 1 4 NI 25 6 1 5 Global Environment Action srrrnnornnnrnnnonnnrrnnnennnrnnnenenrnnnenenrrsnsvennrnnssennnresee 25 62 Model and Suffix Code MS Code ane Seid 26 6 3 Dimension of CSUX1CU saie aea E ena rE EK EA SEEE Eaka ERSE 26 6 4 External Input Qutput erooooonnnnnnnnerernnnnnnnnnnnnerernnnnnnnnnnnnenernnnnnnrnnnnnenenenennnnnvnnee 27 6 5 Command List AA ee ia bisa a 34 IN O A NA 36 1 Safety Precautions 1 1 Introduction Thank you so much for purchasing CSUX1CU Control Unit for CSU This manual covers the features operation methods and procedures safety and handling precautions of the CSUX1CU Before you start to use the unit read this manual thoroughly and operate the unit in a proper manner Please keep this manual in a safe place for future quick reference 1 2 Caution No part of the user s manuals may be transferred or reproduced without prior written consent from YOKOGAWA YOKOGAWA continues
82. rol unit 3 2 2 Filter wheel CSU X1FW Necessary for CSU X1 A1 A2 A3 M1N M1H M2N M2H Part name Part number QTY Description Filter wheel M3918MA 1 Filter wheel unit Aluminum disc M3916MW 6 Aluminum disc to fill open positions in the filter wheel for weight balance Connection cable 1 1 M3918AP 1 2 5m shield cable for filter wheel 1 Connection cable 2 1 M3918AQ 1 2 5m shield cable for filter wheel 2 Filter Wrench M3916CX 1 Tool for exchanging filters in the filter wheel 1 Connection cable 1 is supplied with CSU X1FW 06P 01 and Connection cable 2 is supplied with CSU X1FW 06P 02 3 2 3 Bright Field Control unit CSU X1CU B1 Necessary for CSU X1 A2 A3 Part name Part number QTY Description Bright Field control unit M3918EB 1 Main unit Connection cable 1 M3918AR 1 2 5m shield cable for Bright Field 1 Connection cable 2 M3918AS 1 2 5m shield cable for Bright Field 2 Interlock cable M3918AN 1 2 5m shield cable for Control unit Interlock Communication cable M3918WA 1 Crossing serial cable DSUB 9 pin 1 female female 3m shield cable Controller Interlock key M3918AT 1 Interlock key for control unit 1 Not supplied if you already have filter wheel control unit CSU X1CU F1 AN CAUTION Please ONLY use supplied cables otherwise 1t is possible to fail EMC conformity 27 3 3 Installation For in
83. s e Close to any objects with heavy electromagnetic field e Close to high voltage apparatus or a power line e Under heavy mechanical vibration e Unstable place lt Installation gt Please install the unit as shown below 3 3 2 Connection of equipment Connection with the controller Connect MOTOR1 connector and Filter Wheel 1 with supplied Filter wheel 1 FW1 connecting cable e If necessary connect MOTOR2 connector of Filter Wheel 2 with supplied Filter Wheel 2 FW2 connecting cable e Connect CSU connector of the control unit CSUX1CU F1 and the EXT CONTROL INTERLOCK connector of CSUX1 with the supplied cable Connect the controller interlock key to the EXT INTERFACE LLOCK Connects the RS232C1 connector and PC with supplied RS232C cable After checking the power switch is OFF connect the power cable Please use the power receptacle which fulfills the following conditions 3 pole electric socket equipped with protective earth terminal Rated supply voltage 100 240VAC Power voltage frequency range 90 264VAC Rated supply frequency 50 60Hz Power frequency latitude 48 63Hz Maximum electric power consumption 200VA Please follow the warnings and cautions below before connecting the power supply of a controller If these warning and cautions were not followed there is a possibility of causing an electric shock or breakage of apparatus MN WARNING Please confirm your power supply is suita
84. stallation please follow instructions and installation manual of the microscope laser system fiber coupler camera etc A CAUTION Do not use a different microscope or laser system from the originally installed system If you need to use a microscope or laser system different from the originally installed one please consult Yokogawa Electric Corporation or your local sales representative Please strictly follow Class 3B laser safety instructions in manipulating the optical fiber and optical fiber coupler when you connect to adjust or remove from the laser system or the CSU X1 3 3 1 Installation of Microscope Please follow the instructions given in the user s manual of the microscope manufacturer For confocal imaging laser beam emerges from the objective lens to excite fluorescence of the specimen when CSU X1 is set to the microscope to output a laser beam and the microscope s light path is set for confocal observation Part of the laser beam may be reflected at the specimen s surface and a reflected laser beam may be observed Be careful not to look at the reflected laser beam from the specimen s surface or shield the stage so that it does not go directly into your eyes The reflected laser beam goes through the objective lens and depending on your light path setting it may be observed from the eyepiece of the microscope As the reflected laser beam may come out from the ocular lens of the microscope you must perform the i
85. t 13 MOTOR output Output 14 Source for sensor 24V 15 GND for sensor 33 6 5 Command List Table 6 5 1 Command List Main Item Detailed item Function Command CSU control Shutter Open shutter SHO Close shutter SHC Acquire shutter status SH Acquire open close count SH_CNT Disk Assign disk rotation speed rpm MS n Fine adjustment of disk rotation MS speed to increase Fine adjustment of disk rotation MS speed to decrease Acquire disk rotation speed rpm MS Acquire upper limit of disk MS MAX rotation speed rpm Automatic adjustment to camera MS ADJUST f exposure time ms Run disk rotation MS_RUN Stop disk rotation MS_STOP Dichroic mirror Assign dichroic mirror position DM_POS n Acquire dichroic mirror position DM_POS Filter Wheel control Assign Filter Wheel position FW_POS ch n Acquire Filter Wheel position FW_POS ch Move Bright Field port to the Bright Field control Bright Field Bah BF_INIT original point Port itchi o Eor Eres Switch port to confocal position BF_OFF control Switch port to Bright Field BF_ON position Acquire current position BF_POS Port Assign Port position PT_POS ch n Acquire Port position PT_POS ch External lO control General purpose lO Assign output terminal level O ch n Acquire appointed level of EO chl output term
86. t 4CH External shutter control synchronization control of CSU rotation speed synchronization signal output etc Analogue input 8 ports O 5V 8Bit 0 255 Response 100ms Analogue output 4 ports O 5V 8Bit 0 255 Response 10ms CSU X1CU F1 One control unit controls up to two filter wheels Bright Field Command control of light path switching confocal non confocal Camera port control Switch between the 15 camera port and the 27d camera port CSU X1CU B1 Interlock key 1 Installed Manual shutter switch becomes valid External shutter control from PC through control unit becomes possible 2 Uninstalled Shutter closes motor stops and manual shutter switch becomes invalid Communication 1 Connection Connect control unit cable to the external interlock connector of CSU X1 head Connect control unit and PC through RS232C serial cable Possible to connect and control two control units through daisy chain 2 Conditions Speed 115 200 bps Data bit 8 Parity check None Stop bit 1 Line feed character CR Separating character colon or space Case sensitivity Yes Input 100 240VAC 10 50 or 60Hz 200VAmax 54 Accessory 1 AC100VAC cable 2 Bright Field1connection cable only for CSU X1CU B1 3 Bright Field2connection cable only for CSU X1CU B1 4 CSU connection cable 5 RS232C cable cross 6 Interlock key for control unit 7 1 4 Specifications of Filter Wheel Installable filters Siz
87. t in a safe place so that unauthorized persons are not able to operate the laser For details refer to the user s manual of the laser system you are using in combination of the CSU X1 11 Complies with 21 CFR 1040 10 and 1040 11 except for deviations pursuant to Laser Notice No 50 dated June 24 2007 YOKOGAWA Electric Corporation 2 9 32 Nakacho Musashino shi Tokyo 180 Japan Manufactured KZ Figure 1 2 Positions to attach warning labels to CSU X1 M1 E Labels are the same with the Al model 12 Complies with 21 CFR 1040 10 and 1040 11 except for deviations pursuant to Laser Notice No 50 dated June 24 2007 YOKOGAWA Electric Corporation 2 9 32 Nakacho Musashino shi Tokyo 180 Japan Manufactured KZ Figure 1 3 Positions to attach warning labels to CSU X1 M2 E 13 YOKOGAMA lt gt Complies with 21 CFR 1040 10 and 1040 11 except for deviations pursuant to Laser Notice No 50 dated June 24 2007 YOKOGAWA Electric Corporation 2 9 32 Nakacho Musashino shi Tokyo 180 Japan Manufactured KZ Figure 1 4 Positions to attach warning labels to CSU X1 A2 E WU IA SUPPLYTDLZ MES Y LOOX T ALES 4 VOKOGAWA lt gt Figure 1 5 Complies with 21 CFR 1040 10 and 1040 11 except for deviations pursuant to Laser Notice No 50 dated June 24 2007 YOKOGAWA Electric Corporation 2 9 32 Nakacho Musashino shi Tokyo 180 Japan Manufactured KZ Posit
88. the unit between much different temperature humidity environments In such case please walt for an hour or more until condensation resolved before use lt Ventilation gt Please be careful to secure enough space around the ventilation hole of the unit placed at the motor cover to avoid overheating 11 A CAUTION Please do not install the unit under the following conditions e Under direct sunlight e Close to heating objects e Polluted with oily smoke steam dusts or corrosive gas e Close to any objects with heavy electromagnetic field e Close to high voltage apparatus or a power line e Under heavy mechanical vibration e Unstable place lt Installation gt Please install the unit horizontally 3 3 2 Connection 3 3 2 1 CSUX1CU F1 Model 1 Connect EXT CONTROL INTERLOCK of CSU X1 with the CSU connector of control unit CSUX1CU F1 with supplied cable 2 Connect controller interlock key to EXT INTERFACE1 1 LOCK 3 For communication connect RS232C1 connector of control unit with PC using supplied RS232C cable 4 To use Filter Wheel connect FW1 and MOTOR1 connector with supplied FW1 cable 5 To connect second FW connect FW2 and MOTOR 2 connector with supplied FW2 cable 6 After checking the power switch is OFF connect the power cable Please use the power receptacle which fulfills the following conditions 3 pole electric socket equipped with protective earth terminal Rated supply voltage
89. tion First Camera port Channel No Second Camera Port Please select filters as suffix code Please indicate positions of BA filters to be installed in the filter wheel by numbers 1 to 6 No duplicate number is accepted 59 7 3 Dimension 7 3 1 CSU X1 A1 AA Unit mm Aperture C mount Total width of CSU X1 A1 main unit is 328 5mm including 4mm of the screw head of C mount adapter of main camera port Please connect CSU X1 A1 to microscope with the direct C mount adapter of your microscope 60 7 3 2 CSU X1 A2 AAN ala Y i ke iy 194 Aperture c mount Total width of CSU X1 A2 main unit is 328 5mm including 4mm of the screw head of C mount adapter of main camera port Please connect CSU X1 A2 to microscope with microscope specific adapter made by Yokogawa and supplied as option 61 7 3 3 CSU X1 A3 Xx 304 5 Aperture C mount Total height of CSU X1 A3 main unit is 308 5mm including 4mm of the screw head of C mount adapter of second camera port Please connect CSU X1 A3 to microscope with the direct C mount adapter of your microscope 62 7 3 4 CSU X1 M1 Aperture C mount Total width of CSU X1 M1 main unit is 328 5mm including 4mm of the screw head of C mount adapter of main camera port Please connect CSU X1 M1 to microscope with the direct C mount adapter of your microscope 63 7 3 5 CSU X1 M2 Aperture c
90. u csv yokogawa co jp Tel 81 0 76 258 7028 Fax 81 0 76 258 7029
91. ut TTL CMOS level signal High is Open Low is Close 4 Reserved 5 Reserved 6 Reserved 7 SYNC Sync trigger input signal Input TTL CMOS level 8 INDEX Sync trigger output signal Output TTL CMOS level Pulse width 10ms lt 300Hz 2kHz 9 Interlock _ Open is interlock Input TTL CMOS level 10 Reserved 11 Reserved 12 Reserved 13 PORT M6 port position signal Output TTL CMOS level 14 Reserved 15 Reserved 16 Reserved 17 Reserved 18 Reserved 19 Reserved 20 Reserved 21 SYNC GND 22 INDEX GND 23 Interlock GND 24 Reserved 25 Reserved 26 Reserved 27 PORT GND 28 Reserved 1 Maximum allowable shutter on off frequency is 3Hz for shutter protection If you input faster frequency shutter will not work properly 27 Table6 4 2 PCR68pin male connector of control unit Pin Signal name Function T O Connection No 1 TI1 EI1 1 TTL input ch1 Input TTL level 2 TI2 EI2 1 TTL input ch2 Input 3 TI3 El3 1 TTL input ch3 Input 4 TI4 EI4 1 TTL input ch4 Input a TI H 4 1 2 5 Ik 4 a AOTEA AN 5 VCOM1 con Input For contact input source DCSV 6 VCOM2 Source for contact Input Bee me inputVCOM2 pr E JJ 7 VCOM3 Source for contact Input oe IA input VCOM3 8 VCOM4 Source for contact Input input VCOM4 9 Interlock Open is interlock Input 4Connect with 43pin 10 Reser
92. ved 11 Reserved 12 CSU_SYNC CSU syne signal Input TTL CMOS level input 300Hz 2kHz limitation exists in r the rotation speed CH E gt 13 SHUTTER 5 External shutter Input i ee control signal al H Open L Close 14 OC1 EO1 2 Open collector output Output DC5V 100mA max 1 His ON i oc 15 OC2 E02 2 Dpen collector output Output 2 His ON 16 OC3 EO3 2 Open collector output Output 3N75452B 3 His ON 17 OC4 EO4 2 Open collector output Dutput 1 4 H is ON 18 DO1 EO5 Contact output 1 Output DC30V 500mA max 3 H is ON 5V 19 DO2 EO6 Contact output 2 Dutput la 3 His ON 20 DO3 EO7 Contact output 3 Output gs i 3 H is ON 21 DO4 EO8 Contact output 4 Output 3 H is ON 22 Reserved 23 Reserved 24 Reserved 25 Reserved 28 26 Reserved 27 CSU_INDEX CSU rotation syne Output TTL level signal 300 2kHz TIN A 4 2 1 C qaactsat 2 de se Z 28 31 Reserved 32 34 5V DC5V Output 100mA max 35 GND GND 36 GND 37 GND 38 GND 39 DI1 EI5 4 Photo coupler Input DCSV 7 20mA input 1 CH j 40 DI2 EI6 4 Photo coupler Input a 5 input 2 L 330 TLPTISA 41 DI3 EI7 4 Photo coupler Input input 3 42 DI4 EI8 4 Photo coupler Input input 4 43 Interlock Interlock Input Connect with 9 pin 44 GND 45 GND 46 GND 47 GND 48 GND 49 GND 50 GND 51 GND 52 DO1 Contact output 1 Output D

User`s Manual Confocal Scanner Unit CSU-X1

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