User Manual
Contents
1. _ Program number n Here n can be 1 to 6 Executing this instruction causes program n to beerased and the following commands to be stored as program n Commands are stored until an asterisk followed by CR is sent The asterisk is the last command stored and marks the end of the program for Memowell No facility for loading part of a program is provided The entire program must be loaded at one time Rn Read program n for n between 1 and 6 Executing this instruction causes Memowell to dump the contents of program n over the RS 232 link to the host The Memowell expects no echo each The program is read until the first asterick is encountered signifying the end of the program D Display Memowell revision and unit address 16 Programs can be demonstrated and debugged using the immediate mode issuing N F B commands Each program in Memowell can contain up to 840 characters This corresponds to about 250 commands When full the Memowell will refuse to echo characters The programming can be restarted by another P command The responsibility for keeping track of the program length rests with the host computer The host computer can issue commands to Memowell 1 creating some display pattern Then it can hang up on Memowell 1 and command Memowell 2 to display some independent display pattern Meanwhile Memowell will continue to display as previously commanded A user can interrupt any Memowell that is not in active communications with the ho2. 62 70 78 66 94 07 15 23 31 39 47 5 63 71 79 87 95 08 16 24 32 40 48 56 64 72 80 88 96 Bottom Left Bottom Right Memowell status LEDs are not controllable by the host computer Memowell commands are sequences of ASCII printable characters Each command is terminated by a carriage return CR but no line feed LF The CR causes Memowell to execute the just received command As characters are passed to Memowell Memowell echoes them The terminating CR is echoed as a CR LF The echo of the CR as a CR LF signals the host computer that Memowell has received and executed the just received command The Memowell RS 232 interface consists of the following 5 lines Pin 2 Data from host into Memowell Pin 3 Data from Memowell out to host Pin 4 Request to Send from host into Memowell Pin 5 Clear to Send Used by Memowells to claim use of pin Pin 7 Signal ground A 6V to 12V level on pin 4 signifies that the host is ready to receive characters Pin 5 is used in multi Memowell networks to allow only one Memowell at a time to transmit data back to the host Pin 5 should be ignored by the host The two DB 25 connectors are connected in parallel 15 The Memowell command set is n Attention This command initializes the communications with Memowell Then is the Memowell address used in multi Memowell configurations where 2 to 9 Memowells share a common RS 232 link with the host In this case communications is established with the Memowell tha
3. Memowell User Manual Matrix Technologies Corporation 4 22 Friars Drive 4 Hudson NH 03051 Phone 800 345 0206 4 Fax 603 595 0505 MAN13266 Rev C CONTENTS INTRODUCTION FUNCTION DESCRIPTION PARTS REAR SIDE PANEL BATTERY INSTALLATION ANGLE ADJUSTMENT OPERATION SELECTING LIGHT SEOUENCE TROUBLE SHOOTING MAINTENANCE ORDERING INFORMATION APPENDIX 1 RS232 INTERFACE COMMAND PROTOCOL INTRODUCTION 4 5 Memowell is a 96 well LED lightbox that aids technicians in remembering where next to pipet when working with microwell plates You can choose between six 6 pre programmed functions Single channel light seguences to move via columns Single channel light seguences to move via rows Single channel light seguences to serpentine through columns Single channel light seguences to serpentine through rows Multichannel light sequences lighting 8 LED s at a time Multichannel light sequences lighting 12 LED s at a time OF GV ONS Unique light sequences duplicates triplicates discrete wells etc may be created by using an IBM compatible computer Memowell applications software and downloading via an RS232 port on the rear of the instrument Automatic light advances may be accomplished by connecting the Memowell to the console of the Electrapette Each time the pipet trigger is pressed and liquid manipulated the lights will automatically advance to the next series of wells to be pipetted Lights can also be advanced when
4. le You want to dispense liquid into a microtiter plate using an eight channel pipettor Press the Standard key five times until number 5 is illuminated Press the Enter key and the first column will be illuminated Note Use of the Program key requires the RS232 port and the commands in Appendix I This allows you to create your own light sequences e g dispensing of duplicate samples etc 11 TROUBLE SHOOTING PROBLEM SOLUTION The power does not work 1 Check the battery installation 2 Recharge the batteries 3 Connect the unit to a power outlet via the transformer The Memowell does not work in 1 Check your connection on the rear of conjunction with Electrapette the Electrapette The correct port has not been connected or is not fully connected The LED s are moving in reverse direction 1 Press the Reverse key to cancel the reverse movement of the light seguence 12 MAINTENANCE Note Do not leave the Memowell exposed to U V light for extended periods Extended exposure will cause discoloration of the plastic housing To clean the instrument use a soap solution or methanol and gently wipe off the keyboard To recharge the batteries connect the transformer to the rear of the instrument To recharge batteries to full capacity will take approximately 14 16 hours A guick charge of 6 hours will result in approximately 4 hours of operation In the event that something not addressed in this manual sho
5. st by selecting a program This protocol is subject to change 17
6. start to blink when approximately 20 30 minutes of power remain PARTS Your package should include the following parts 1 2 The Memowell Catalog No 5000 Transformer Catalog No 8098 2 Extra long screws for adjusting the angle of the Memowell Communication cord for use with Electrapette Console Catalog No 5004 Users Manual 6 Rechargeable AA batteries Optional in U S Catalog No 5002 REAR SIDE PANEL RS 232 Port Port for footswitch or Inlet for power supply Electrapette to Memowell communications cord BATTERY INSTALLATION Note Battery operation is optional Before you start using the Memowell make sure that you have installed 6 AA rechargeable nickel cadmium batteries Open the Memowell by loosening the four screws at the bottom of the unit Lift the lid of the instrument and tilt it forward Correctly orient the batteries in the battery holder Close the unit and tighten the four screws Batteries should be charged for 14 16 hours upon installation to fully charge This is accomplished using the power transformer included with the Memowell The batteries are charged the fastest with the power in the off mode Si Se DD The Memowell may be operated immediately by using the power transformer Caution Using non rechargeable batteries can cause SEVERE damage to the Memowell ANGLE ADJUSTMENT To adjust the angle of the Memowell please use the supplied extra long screws with plastic b
7. t has been previously assigned address n Memowell n responds by echoing the n its address and a colon The default address for all Memowells is 0 A 0 address Memowell will respond to any addressed attention Also the attention command issues with an address of 0 0 CR will cause every Memowell on the network to respond a mess if there are actually multiple Memowells on line X Hang up character This character terminates the communications with the active Memowell As soon as the command is received the active Memowell will go off line The Memowell echoes the X then echoes the CR as a CR LF An Assign address n This command assigns the address n to a single Memowell for later use in a Multi Memowell configuration The value of n can be 1 to 9 The default address is 0 for use only on single Memowell configurations Executing the A command on a Multi Memowell configuration will cause mass confusion as all Memowells will be assigned the same address The host program must ensure that this command is not issued into a Memowell network If it is the network will have to be disassembled and Memowells put on line individually for reassignment of addresses 0 Clear the display Turn off LEDs 1 through 96 Nn _ Turn on LED n Here n can be between 1 and 96 Fn Turn off LED n n between 1 and 96 Bn Blink LED n n between 1 and 96 T Sound the Tone Wn Wait unconditionally for n units of time S Wait for a footswitch depression Pn
8. uld go wrong or technical guestions arise Matrix Technologies Technical Services Department is available to address your needs Please contact Technical Service Department Matrix Technologies Corporation 22 Friars Drive Hudson NH 03051 800 345 0206 or 603 595 0505 In Europe Technical Service Department Matrix Technologies Corporation 13 Croft Road Wilmslow Cheshire UK SK9 The Hollies 44 1625 549 036 13 ORDERING INFORMATION CATALOG NO 9000 9001 9002 9003 9004 DESCRIPTION Memowell Lightbox microplate memory device includes Electrapette connection cable leveling feet and a Swiss Army Knife Footswitch for Memowell or Electrapette Console Rechargeable Memowell Batteries 6 pack Serial Communications Cable Electrapette Connection Cable Memowell RS 232 INTERFACE Command Protocol 14 Rev 1 00 12 19 89 Applies to Memowell Rel 1 00 This is a protocol for commanding the Memowell via the RS 232 port The Memowell is commanded either for immediate execution or for program mode commands are stored for later execution Each LED has a unigue identifier address for programming The LED identifiers are as follows Iooking down on the face of Memowell Top Left Top Right 01 09 17 25 33 41 49 S57 65 73 81 89 02 10 18 26 34 42 50 58 66 74 82 90 03 11 19 27 35 43 51 59 67 75 83 9 04 12 20 28 36 44 52 60 68 76 84 o 05 13 21 29 37 45 5 amp 3 61 69 77 6 93 06 14 22 30 38 46 54
9. ushings Simply unscrew the short screws found on the Memowell and replace with the supplemental long screws with plastic bushings The Memowell can be leveled when these long screws are installed in the front of the unit OPERATION The Memowell is ready for use with charged batteries and or with power from the wall transformer There are three ways to operate the unit 1 In conjunction with an Electrapette Connect the Memowell with the Electrapette by using the black communications cord supplied 2 Utilizing a footswitch optional in conjunction with an Impact or manual pipettor 3 Using the step keys on the Memowell keyboard when using an Impact or manual pipettor 10 SELECTING LIGHT SEOUENCE With only a few keystrokes you can instruct the Memowell to illuminate the appropriate well seguence There are six pre programmed options of light seguences Single channel dispensing in columns from A H Single channel dispensing in rows from 1 12 Single channel dispensing in serpentine pattern A H H A etc Single channel dispensing in serpentine pattern 1 12 12 1 etc Multi 8 channel dispensing Multi 12 channel dispensing D OE ols Before selecting your light sequence make sure the power has been turned on To select your light sequence continue to press the Standard key until you reach your choice for a pre programmed light sequence options 1 6 When your choice is illuminated press the Enter key Examp
10. using a manual footswitch Memowell is A C powered or available with optional rechargeable batteries for operation under laminar flow hoods Memowell can also be ganged together serially with other Memowells allowing you to track liquids transferred from one plate to another FUNCTION DESCRIPTION Single Channel dispensing in columns from A H Single channel dispensing in rows from 1 12 Single channel dispensing in serpentine pattern A H H A etc Single channel dispensing in serpentine pattern 1 12 12 1 etc Multi 8 channel dispensing Multi 12 channel dispensing N gt Standard Allows you to choose any of the above pre programmed functions 1 6 Program Permits you to recall or store up to 6 custom program Enter Is used to confirm your Standard or Program selection 1 6 Step These keys located on left and right hand side of the keyboard allow you to advance your light sequence by pressing whether of the keys If you keep the key depressed the LED s will advance rapidly If key is pressed once only the LED will advance one well at a time Reverse Reverses the direction of your pipetting sequence When the instrument is in the reverse mode the LED above the reverse key is illuminated FUNCTION Tone Activates or deactivates the beep tone by alternately pressing this key Power Turns the instrument on and off Recharge The LED starts to blink when it is time to recharge the battery The LED will
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