DOWNLOAD NOW Borealis User Manual User
Contents
1. an Oxford Instruments company Borealis Version 2 0 revised 03 Mar 2015 User Guide covering Borealis enhanced CSU X and CSU W models andor com Andor Technology 2015 ANDOR company TABLE OF CONTENTS SECTION 1 INTRODUCTION eere eee eene 13 Ib TECHNICAL 14 12 BDISGLAIMED 15 1 3 COPYRIGHT AND PROTECTIVE NOTICES 15 1 4 TRADEMARKS AND PATENT INFORMATION 15 1 5 SUPPLIED 16 Lo OPTIONAL 17 SECTION 2 PRODUCT OVERVIEW 18 21 BEAM CONDITIONING UNIT BCU ee e ceeeeeee enne nnne nnn nnns 18 2 1 1 seiner 18 2 OMOR mn 18 Ge TOUR EE 19 2 FIDIO LOCK 19 21 5 BCU Fibre 19 2 150 Power SUDD 19 22 BOREALIS ENHANGED CSU E 20 221 COU FIDE WU EE 21 2 22 Field Lens 08 eege 21 2 2 9 BelloWS AGIDO NES 21 224 eet lee TE 21 2 9 ET e 21 24 Oo TEM OVERVIEW eebe 22 9 9 Version 2 02. e 29 61 TROUBLESHOOTING 29 6 1 1 No Laser Power at ee 29 6 1 2 Low Laser Power at 50 ENEE NEEN EEN 29 6 1 3 Poor Uniformity over CSU 29 6 2 PROBLEM REPORTING 30 SECTION 7 TECHNICAL SPECIFICATIONS 31 7 1 BEAM CONDITIONING UNIT BCU nennen nnn nnn nnns 31 7 2 BOREALIS ENHANCED 31 FIBEEDEENS EEN 31 7 4 BELLOWS 31 7 5 31 APPENDIX A MECHANICAL DRAWINGS 32 APPENDIX B GLOSSARY 34 APPENDIX C OTHER INFORMATION EN KK KN KK
3. fibre optic output cable Borealis On Off switch Figure 4 The Borealis Beam Conditioning Unit 2 1 1 EMISSION INDICATORS Emission LEDs on the front panel of the BCU show the status of the unit When these are lit laser emission is possible Please note there are two LEDs to provide redundancy for the laser emission status 2 1 2 ON OFF SWITCH A toggle switch is located on the front panel of the BCU This controls the power to the unit Version 2 0 rev 03 Mar 2015 1 8 PRODUCT OVERVIEW Spectral Applied exxarch Ir Andor Technology Model BCLI NNN ta Unit 11 Serial No 210000N ON Canada 148 318 Fibre lock 0 www spectral ca Manufactured 0004 2014 ples with IEC GO RZ3 1 200 10 and 1040 77 mr deviation shal a martin Loser gated HITIA Fibre input Power Power input E Laser Input 2 Figure 5 Rear panel of the Beam Conditioning Unit 2 1 3 POWER INPUT A 2 1 mm input connector is used to provide DC power from the power supply unit to the BCU 2 1 4 FIBRE A locking mechanism is provided on the rear panel of the BCU that closes over the MM fibre coming from the laser source This fibre lock must be put in place at time of installation by the installer WARNINGS e NEVER REMOVE THE FIBRE LOCK WHEN IN OPERATION e THE CABLE SHOULD ONLY BE DISCONNECTED BY QUALIFIED SERVICE PERSONNEL A TOOL IS REQ
4. a temporary conductivity caused by condensation must be expected Ventilation Requirements Do not cover during operation T Version 2 0 rev 03 Mar 2015 31 MECHANICAL DRAWINGS ANDOR company APPENDIX A MECHANICAL DRAWINGS Borealis Conditioning Unit BCU 200mm Field lens tubes 1 0x Short 37 6 mm 109 3 i 187 mm Bellows coupling adaptor CSU X Bellows coupling adaptor CSU W 38 2 mm 60 8 mm 28 mm 93 2 mm 19690999949 AU 104044009 28 mm Version 2 0 rev 03 Mar 2015 32 ANDOR company MECHANICAL DRAWINGS 3 position dichroic module for manual CSU models 84 77 mm 64 61 mm 142 43 mm Borealis enhanced CSU W Borealis components dimensional information Note refer to the Yokogawa CSU X1 and CSU W1 manuals for further dimensional information on the standard units Version 2 0 rev 03 Mar 2015 33 GLOSSARY ANDOR company APPENDIX B GLOSSARY AC AC DC ADC AEL BCU CSU CLSM DC Diffuse Reflection EMC ESD EU FC APC lO ILE Intrabeam Viewing LED LMM MM NOHD MPE Parfocal Specular Reflection Alternating Current Mains An electronic device that conv
5. 3B and below or 3 ability of ahuman hand or arm to intercept levels of radiation above the AEL of Class 3B 4 also for levels of radiation within the protective housing that are equivalent to Class 3B or Class 4 ability of any part of the human body to meet hazardous laser radiation that can be reflected directly by any single introduced flat surface from the interior of the product through any opening in its protective housing The standard Ophir PD 300W sensor and power meter can be used to assess accessible power levels in conjunction with suitable Safety Glasses and applicable Safe Systems of Work Any queries should be referred to the Andor Product Laser Safety Officer for guidance lf Human Access is not possible then the device should be reclassified as a Class 1 device This involves the following steps 1 Remove all other Explanatory Classification Labels EXCEPT those on the Laser Sources these count as Removable Laser Sources and need to remain labelled and classified as stand alone products 2 Fita Class 1 Explanatory Label onto a permanently affixed surface which is easily visible before and during operation of the system 3 Ensure a Laser Hazard Symbol is clearly visible before and during operation and affix a label if not Version 2 0 03 2015 24 ANDOR company OPERATION SECTION 4 OPERATION WARNINGS e IF THE EQUIPMENT IS USED IN A MANNER NOT SPECIFIED BY SPECTRAL OR ANDOR SYSTE
6. company MAINTENANCE SECTION 5 MAINTENANCE WARNINGS e THERE ARE NO USER MAINTENANCE PROCEDURES REQUIRED FOR THE BCU OR CSU THE SYSTEM SHOULD BE POWERED DOWN PRIOR TO USER PERFORMING ANY MAINTENANCE CHECKS e DO NOT USE EQUIPMENT THAT IS DAMAGED e CONTACT YOUR ANDOR REPRESENTATIVE IF THERE ARE ANY QUERIES OR ISSUES WITH YOUR BOREALIS SYSTEM 5 1 CLEANING AND DECONTAMINATION The most critical aspect of maintenance by the user is to ensure that the system is in a clean environment that is suitable for sensitive electro optical equipment The laboratory should be free of dust fumes and other materials that could affect the system e To clean the product only use a damp lint free cloth on the external housing of the unit Do not wet the connectors Use water only do not use solvents cleaning agents or aerosols 5 2 REGULAR CHECKS The state of the product should be checked regularly especially the integrity of the enclosure and the mains cable On a Daily Basis e Visually inspect the system Perform any maintenance activities suggested by the microscope and camera manufacturer s On a Weekly Basis e Ensure that all power cables are firmly in place Check the optical cables connections and interlocks to ensure that the fibre shroud locksare in place and no damage has occurred to the optical fibres connecting the various elements of the system 5 3 ANNUAL ELECTRICAL SAFETY CHECKS It is advisabl
7. rev 03 Mar 2015 2 ANDOR company SECTION dE Eege 23 3 1 LOCATION AND 23 S2 gue 23 zc cn XX OE 23 94 INTEREOCGK S eee eee 23 3 5 CLASSIFICATION OF AN INSTALLED SYSTEM 24 SECTION 4 OPERATION KNEE ENE RITU YE KH ME RES 25 41 EMERGENCY MAINS 5 25 42 2 25 AS POWEHR DOWN SEQUENCE 26 eee nee ee ee 26 4 4 1 Mechanical 5 26 4 4 2 Hazards Due to Moisture or 26 45 USING THE EECHER 27 USET e ele VE mI MM 27 4 5 2 Using the CSU Dichroic Slider not applicable to 5 27 SECTION 5 MAINTENANTQCGFE Leere ENEE RENE Ree 28 51 CLEANING AND nennen nn nnns 28 92 28 5 3 ANNUAL ELECTRICAL SAFETY 28 2 0 03 2015 3 ANDOR company SECTION 6 TROUBLESHOOTING
8. serviceable parts inside the product and the enclosure must not be opened Only authorised service personnel may service this equipment This product will be used with lasers IEC Technical Document IEC TR 60825 14 recommends the presence of a Laser Safety Officer 050 however national guidelines should be referred to This equipment has not been designed and manufactured for the medical diagnosis of patients Do not attempt to bypass any safety interlocks They are provided to comply with the safety requirements of various regulatory agencies and must be employed to protect the operator Protective earth is an integral part of the protection against electric shock in this product and is provided via the earth pin of the external power supply Ensure that this is plugged into the building earth system via the mains socket Do not tamper with any of the earthing measures Only the correctly specified mains supply should be used Only the AC DC external power supply provided with the product should be used Only the power supply cord provided with the product should be used Should this not be correct for your geographical area contact your local Andor representative Make sure the power supply cord is located so that it will not be subject to damage If replacement of the detachable power supply cord is required ensure replacement is of same type and rating Locate the laser source BCU and confocal head within 2 m of ea
9. 35 Version 2 0 03 2015 4 ANDOR Revision History Rev A 9 Dec 2013 Initial Release 1 0 28 May 2014 Updated presentation to Andor format and enhanced content all Sections Updated and additional laser safety information Safety and Warning Section Information on dichroic slider added Sections 2 2 4 and 4 5 2 Glossary added Appendix B 2 0 03 Mar 2015 Updated to include the CSU W borealis model Version 2 0 rev 03 Mar 2015 5 ANDOR company Safety and Warning Information 1 PLEASE READ THIS INFORMATION FIRST If the equipment is used in a manner not specified by Spectral or Andor the protection provided by the equipment may be impaired CAUTION USE OF CONTROLS OR ADJUSTMENTS OR PERFORMANCE OF PROCEDURES OTHER THAN THOSE SPECIFIED HEREIN MAY RESULT IN HAZARDOUS RADIATION EXPOSURE 2 12 13 14 T7 18 Do not position this product so that it is difficult to operate the Mains disconnecting device See SECTION 4 1 Emergency Mains Disconnection Before using the system please follow and adhere to all warnings safety manual handling and operating instructions located either on the product or in this manual Keep this manual in a safe place for future reference Users must be authorised and trained personnel only otherwise this may result in personal injury and or equipment damage and impaired system performance There are no user
10. M DISTRIBUTORS THE PROTECTION PROVIDED BY THE EQUIPMENT MAY BE IMPAIRED e READ THE USER GUIDES SUPPLIED WITH YOUR SYSTEM COMPONENTS AND SOFTWARE PRIOR TO USE 4 1 EMERGENCY MAINS DISCONNECTION In case of emergency the disconnecting point of the equipment is the mains power cord connected to the external power supply or the mains socket switch WARNING SWITCH OFF THE POWER AT THE MAINS SOCKET AND REMOVE THE MAINS LEAD FROM THE EXTERNAL POWER SUPPLY 4 2 POWER UP SEQUENCE Once all system components are connected it may be turned on as follows 1 Turn on the CSU using the key switch refer to the manufacturer s instructions CSU X EXIT CONTROL INTERLOCK DCIN ON PO CSU ON OFF Key Switch CSU W CSU ON OFF Key Switch Shutter Version 2 0 rev 03 Mar 2015 25 ANDOR company OPERATION 2 Turn on the BCU using the front panel toggle switch Laser Emission Emission Status LEDS output KEE BCU ON OFF Toggle Switch ue d Confirm that the Emission LEDs light Turn on laser source according to manufacturer s instructions The system can now be used as outlined in the user guides for your CSU equipped Confocal system ee um Refer also to your user guides supplied with your software e g Andor iQ 4 3 PowerR DOWN SEQUENCE In order to turn the system off proceed as follows 1 off the laser source 2 Turn off the CSU using t
11. RKS AND PATENT INFORMATION Spectral Applied Research Inc is a Division of Andor Technology Andor and the Andor logo are trademarks of Andor Technology Andor Technology is an Oxford Instruments company All other marks are property of their owners Borealis includes technology covered by the following patents US Patent No 8 275 226 EP Patent No 2196839 US Patent No 8 670 178 and CA Patent No 2779146 Changes are periodically made to the product and these will be incorporated into new editions of the manual New versions of all Andor manuals will be made available through MyAndor http my andor com login aspx If you do not have an account please register at http my andor com Register aspx T reve Version 2 0 rev 03 Mar 2015 15 ANDOR company INTRODUCTION 1 5 SUPPLIED COMPONENTS Description Quantity Borealis enhanced CSU Typically existing CSU X1 or CSU W1 model fitted with Borealis 1 upgrades or supplied with Revolution XD system Beam Conditioning Unit BCU Includes Multi mode Fibre 2 metre for connection to CSU Includes external power supply Field Lens Tube size as selected at time of ordering Bellows Adaptor Microscope specific Multi mode Fibre 2 metre For connection of laser source to BCU User Guide on CD Notes 1 Manual CSU model may be or
12. Tokyo 101 0064 Chaoyang District Japan Beijing 100101 PR Tel 81 3 3518 6488 China Fax 81 3 3518 6489 86 0 10 51294977 Fax 86 0 10 6445 5401 www andor com contact_us support_request www andor com contact_us support_request The latest contact details for your local representative can be found on our website Version 2 0 03 2015 14 ANDOR company INTRODUCTION 1 2 DISCLAIMER THE INFORMATION CONTAINED HEREIN IS PROVIDED AS IS WITHOUT WARRANTY CONDITION OR REPRESENTATION OF ANY KIND EITHER EXPRESS IMPLIED STATUTORY OR OTHERWISE INCLUDING BUT NOT LIMITED TO ANY WARRANTY OF MERCHANTABILITY NON INFRINGEMENT OR FITNESS FOR A PARTICULAR PURPOSE IN NO EVENT SHALL ANDOR BE LIABLE FOR ANY LOSS OR DAMAGE WHETHER DIRECT INDIRECT SPECIAL INCIDENTAL CONSEQUENTIAL OR OTHERWISE HOWSOEVER CAUSED WHETHER ARISING IN CONTRACT TORT OR OTHERWISE ARISING OUT OF OR IN CONNECTION WITH THE USE OF THE INFORMATION PROVIDED HEREIN 1 3 COPYRIGHT AND PROTECTIVE NOTICES The copyright in this document and the associated drawings are the property of Andor Technology and all rights are reserved This document and the associated drawings are issued on condition that they are not copied reprinted or reproduced nor their contents disclosed The publication of information in this documentation does not imply freedom from any patent or proprietary right of Andor Technology or any third party 1 4 TRADEMA
13. UIRED TO OPEN THE LOCKING MECHANISM ONCE CLOSED 2 1 5 BCU FIBRE INPUT A MM fibre is provided to connect the laser source to the BCU This connection is made to the fibre input connection on the rear panel of the BCU using the FC connector provided and locked in place with the fibre lock see Fibre Lock description above 2 1 6 POWER SUPPLY The BCU is supplied with a 12V DC 2A certified power supply Do not use any other power supply PSU Specifications Input 90 264 V AC 47 63 Hz Output Typ 2A Figure 6 BCU Power Supply Version 2 0 rev 03 Mar 2015 1 9 ANDOR company 2 2 BOREALIS ENHANCED CSU The Borealis Enhanced CSU features proprietary modifications to enhance the input and illumination optics as part of the Borealis upgrade These enhancements cover CSU models CSU 10 CSU 21 CSU 22 the CSU X1 shown in figure 7 and the CSU W1 shown in figure 8 The operation and the existing functionality of the original model of CSU are retained This includes key switch operation shutter behaviour and access panel interlocks Please refer to the supplied manuals for general information on the standard CSU units Field lens tube Fibre Input 9 Guard Bas Bellows adaptor Figure 7 Borealis Enhanced CSU X shown with Field Lens tube and Bellows Adaptor Figure 8 Borealis Enhanced CSU W Please note that the Borealis Enhanced CSU W has an Interlock Output Connector that must be interf
14. aced to the Laser Controller to ensure that the device s safety behaviour is maintained Version 2 0 03 Mar 2015 20 ANDOR company PRODUCT OVERVIEW 2 2 1 CSU FIBRE INPUT CSU X The CSU X fibre input coupler has been revised to permit the use of the square core MM output fibres of the BCU A guard protects the fibre coupling and must be in place during normal operation CSU W The Borealis enhanced CSU W includes a fibre lock mechanism to secure the square core MM output fibre of the BCU This fibre lock must be in place during operations 2 2 2 FIELD LENS UBE In a Borealis enhanced system a range of field lens tubes are available to enable adjustment of the effective pinhole size Adjustment of the pinhole size allows the CSU to be used with lower magnification objective lenses and take advantage of their longer working distances The Field Lens Tube is inserted into the optical path in between the microscope bellows adaptor and the CSU device as shown in Figure 7 2 2 3 BELLOWS ADAPTOR The bellows adaptor replaces the standard rigid C mount connection to the microscope The bellows adaptor facilitates strain free alignment and connection of the CSU to the microscope Adaptors are specific to the microscope model and are compatible with the Andor XD tilt stage and Andor WD mounting kit 2 2 4 CSU DICHROIC SLIDER The optional 3 position dichroic slider unit replaces the single position excitation dichroic holder of
15. ample is shown below The appropriate aperture label is included with the documentation for application by the System Integrator Figure 3 Example of Laser emission warning label on microscope DESCRIPTION OF EMITTED RADIATION FROM THE LASER PRODUCT Wavelengths 400 800 nm Exact outputs will depend on the integrated laser source please refer to it s Explanatory Label and User Documentation Beam Divergence 0 3 1 4 NA Exact divergence will be dependent on the objective in use please refer to the microscope s User Documentation Maximum Power or lt 500 mW The maximum output power will depend on the integrated laser source and the configured optical elements but will be significantly energy Output less than 500mW Pulse Duration N A All recommended laser sources for the Borealis Product are Continuous Wave output Pulse Repetition N A All recommended laser sources for the Borealis Product are EE Continuous Wave output Irregular Pulse N A All recommended laser sources for the Borealis Product are Pattern Continuous Wave output DESCRIPTION OF EMITTED RADIATION FROM THE INTEGRATED LASER SOURCE This is dependent on the laser source integrated with the Borealis system As there are multiple options available from Andor or Borealis may be integrated with a customer s existing laser source please refer to the Laser Source s Explanatory Label and User Documentation for a description of its Emitted Radiation _ 6 4 Vers
16. cess to the area s where laser s are in use to those persons who are trained in the dangers of lasers and trained on the safety precautions to be observed when working with lasers Ensure suitable laser warning signs are prominently displayed in the area the system operates If the system is not in use turn the laser off using the key switch On a daily basis or before every use verify that the laser interlock circuit is working by confirming that the laser emission indicator on the source turns off when any of the following are done The microscope binocular eyepieces are in the open position e The articulated transmitted light arm on inverted microscopes is tilted back from the functional vertical position before using the system Fluorescent cards should be used to visually locate and indicate the output of invisible wavelengths at all times Additional precautions may need to be implemented as the necessary precautions will be specific to each Borealis System s installed configuration and typical mode of use The responsible Laser Safety Officer must assess and implement the necessary precautions to avoid possible exposure to hazardous radiation during use A E Version 2 0 rev 03 Mar 2015 11 ANDOR company EYE PROTECTION Eye Protection is not required for the safe use of the device as the only radiation observable without intentional misuse is directionally stable diffuse and highly divergent from the designated aper
17. ch other so that the interconnecting fibres are not stretched bent or constrained While running an experiment try to keep room temperature as stable as possible Performance of the system may be adversely affected by rapidly changing environmental conditions or operation outside of the operating conditions specified in SECTION 7 TECHNICAL SPECIFICATIONS E 9 9 Version 2 0 rev 03 Mar 2015 6 ANDOR company 19 Electromagnetic Compatibility This is a Class A product In a domestic environment this product may cause electromagnetic interference in which case the user may be required to take adequate measures 20 This product has been designed and tested to perform successfully in a normal basic electromagnetic environment e g a typical life science test laboratory as per the EU EMC Directive It is not designed to operate in a harsh electromagnetic environment e g close to the following equipment EMI RFI generators electrostatic field generators electromagnetic or radioactive devices plasma sources arc welders x ray instruments intense pulsed sources or other similar sources of high energy fields whose emissions are not within the normal range expected under the EU EMC Directive 21 Your product is a precision scientific instrument containing fragile components Always handle it with care 22 When transporting the BCU ensure that the fibre cables are not bent tightly as this may damage the internal optica
18. dered with 3 position Dichroic Slider see Section 1 6 2 Additional Field Lens Tubes may be supplied as ordered see Section 1 6 Version 2 0 rev 03 Mar 2015 16 ANDOR INTRODUCTION 1 6 OPTIONAL COMPONENTS There are a range of optional components available for the Borealis including Additional field lens tubes to adjust pinhole magnification and working distances 3 position dichroic slider option for manual CSU models only dichroic options installed as specified at time of ordering Please contact your nearest Andor Sales Representative for further information on these and other system components available Version 2 0 03 2015 17 ANDOR company PRODUCT OVERVIEW SECTION 2 PRODUCT OVERVIEW This section provides an overview of the main Borealis system components e Beam Conditioning Unit BCU e Borealis Enhanced CSU including field lens tube and bellows adaptor 2 1 BEAM CONDITIONING UNIT BCU The BCU homogenizes the laser beam output from the laser engine multi mode fibre It is coupled to the laser source through a MM fibre with fibre optic couplings FC APC The output fibre FC APC is of the armoured type and not removable and connects to the Borealis Enhanced CSU The MM output fibre has a square core cross section which is ideal for use with square format cameras e g iXon Ultra 897 or Zyla 4 2 Emission LEDs E ANDOR
19. e to check the integrity of the insulation and protective earth of the product on an annual basis e g U K PAT testing Version 2 0 03 2015 28 ANDOR company TROUBLESHOOTING SECTION 6 TROUBLESHOOTING 6 1 TROUBLESHOOTING EXAMPLES The following examples provide guidance for some possible troubleshooting scenarios Refer also to the operation and troubleshooting information provided with your other system components 6 1 1 No LASER Power ar CSU Action 1 If the Emission LEDs are not lit check BCU power supply 2 f the Emission LEDs are lit Check the laser source refer to manufacturers service information No laser power at CSU Check the light path settings e g filter cubes in the microscope filter turret Check all interlocked panels are present and fixed securely Check that the software has successfully connected to the Hardware and all required shutters are open 6 1 2 Low LASER Power AT CSU Fault Action Low laser power at CSU 1 Contact your customer support representative 6 1 3 Poor UNIFORMITY OVER CSU FOV Fault Action Poor uniformity over CSU 1 Contact your customer support representative FOV _ _ _____ oe Version 2 0 rev 03 Mar 2015 29 ANDOR TROUBLESHOOTING 6 2 PROBLEM REPORTING FORM Please have the following information when connecting customer support Owner Information Institution Name Vendor Information f
20. elengths Nominal Ocular Hazard Distance Distance from the output aperture at which the beam irradiance or radiant exposure equals the appropriate corneal maximum permissible exposure MPE Maximum Permissible Exposure Level of laser radiation to which under normal circumstances persons may be exposed without suffering adverse effects The MPE levels represent the maximum level to which the eye or skin can be exposed without consequential injury immediately or after a long time and are related to the wavelength of the laser radiation the pulse duration or exposure duration the tissue at risk and for visible and near infra red laser radiation in the range 400 nm to 1 400 nm the size of the retinal image Maximum permissible exposure levels are in the existing state of knowledge specified in Annex A of IEC 60825 1 When optical components such as the objective lenses or camera ports of a microscope are described as parfocal this means that they may be interchanged without affecting the focus on the image Reflection from a surface that can be considered a beam including reflections from mirrored surfaces OOO Version 2 0 rev 03 Mar 2015 34 ANDOR company OTHER INFORMATION APPENDIX C OTHER INFORMATION TERMS AND CONDITIONS OF SALE AND WARRANTY INFORMATION The terms and conditions of sale including warranty conditions will have been made available during the ordering process The current version may be viewed a
21. erts AC electricity usually mains into a DC voltage usually a safe low voltage Analogue to Digital Converter Converts an analogue voltage to a digital signal Accessible Emission Limit The maximum accessible emission permitted within a particular class Wherever the text refers to emission level not exceeding AEL or similar wording it is implicit that the accessible emission is determined following the measurement criteria specified in Clause 9 of IEC 60825 1 Beam Conditioning Unit Homogenizes the light from the laser source to permit uniform illumination across the image Confocal Spinning Disk Confocal Laser Scanning Microscopy Direct Current Change of the spatial distribution of a beam of radiation by scattering in many directions by a surface or medium Electromagnetic Compatibility Electrostatic Discharge European Union Fibre Optic connector Angled Physical Contact Input Output Generic input and output electrical signal connections Integrated Laser Engine Control for up to 4 individual lasers All viewing conditions whereby the eye is exposed to the direct or specularly reflected laser beam in contrast to viewing of for example diffuse reflections Light Emitting Diode Laser Merge Module Combines multiple laser wavelengths into a single fibre optic cable output Multi mode fibre Multi mode fibres have a much larger core diameter than single mode fibres This enables improved transmission of longer wav
22. erview of Borealis enhanced CSU W as part of an overall system Version 2 0 rev 03 Mar 2015 22 ANDOR company INSTALLATION SECTION 3 INSTALLATION WARNINGS e THE BOREALIS SYSTEM MUST BE INSTALLED BY AN AUTHORIZED INSTALLATION ENGINEER ACCORDING TO THE INFORMATION PROVIDED BY SPECTRAL AND ANDOR ANY INSTALLATION STEPS INVOLVING LASERS MUST BE PERFORMED BY QUALIFIED PERSONNEL USING PERTINENT LASER SAFETY PROTOCOLS e SETUP AND OPERATION OF OTHER SYSTEM COMPONENTS ARE DESCRIBED IN THEIR RESPECTIVE GUIDES 3 1 LOCATION AND MOUNTING e Temperature and humidity must meet the specifications defined in SECTION 7 e Operational vibrations should be reduced as much as possible for stability of the imaging train Power cabling and control cables should be routed to prevent accidents damage and accidental unplugging while avoiding bend radii of less than 30 mm 3 2 VENTILATION Do not cover equipment during operation 3 3 ASSEMBLY This product requires no assembly and is recommended to be installed by an installation engineer authorized by Spectral Andor or authorized distributor INTERLOCKS CSU X The CSU X uses an internal shutter to block the laser path when the key switch is turned OFF CSU W In the CSU W the internal shutter has been disabled but this function has been replaced so that the interlocking to the laser source provided by the Interlock Output connector will shut off the laser source when a pro
23. exposure Viewing diffuse reflections is normally safe Class 3B lasers which approach the AEL for Class 3B may produce minor skin injuries or even pose a risk of igniting flammable materials However this is only likely is a small focused spot Note There exist some theoretical but rare viewing conditions where viewing a diffuse reflection could exceed the MPE For example for Class 3B lasers having powers approaching the AEL lengthy viewing of greater than 10 s of true diffuse reflections of visible radiation and viewing at distances less than 13 cm between the diffusing surface and the cornea can exceed the MPE Class 4 Laser products for which intrabeam viewing and skin exposure is hazardous and were viewing of diffuse reflections may be hazardous These lasers also often represent a fire hazard A 29 0 Version 2 0 rev 03 Mar 2015 9 ANDOR company LASER APERTURE The following label is attached to the end of the optical fibre output cable of the BCU These labels indicate that during installation laser radiation may be emitted from the optical fibres when disconnected When properly installed no laser radiation will be emitted from these points during use Figure 2 Laser aperture warning label on BCU optical fibre output The System Integrator MUST ensure that the final system s Laser Aperture is suitably labelled e g the microscope s Objective is identified by a label on the microscope s stage top An ex
24. gramming command is received to close the shutter or the Keyswitch is turned OFF The Interlock Output connector must be connected with an appropriate cable to the laser source to ensure this functionality T a Version 2 0 rev 03 Mar 2015 23 ANDOR company INSTALLATION 3 5 CLASSIFICATION OF AN INSTALLED SYSTEM The proposed installation scheme of all systems is captured and assessed for all orders received by Andor To breach the Class 4 limits for accessible emissions due to known standard attenuations the input laser power would have to be in excess of 5W We know this configuration is not possible at present and can therefore safely classify the majority of systems at Factory QC as Class 3B products The only case to re assess the accessible emissions if is the beam path appears to be fully enclosed The following test criteria should be applied if a system is felt to be fully enclosed e g uses a stage cover and or environmental enclosure Assess if any of the laser emissions are open to Human Access as defined by 1 ability of the human body to meet laser radiation emitted by the laser product 1 radiation that can be intercepted outside of the protective housing or 2 ability of a cylindrical probe with a diameter of 100 mm and a length of 100 mm to intercept levels of radiation of Class
25. he key switch 3 Turn off the BCU using the front panel toggle switch NOTE THE BCU SHOULD BE POWERED OFF WHEN NOT IN USE TO PRESERVE MOTOR LIFE 4 4 Risk MITIGATION 4 4 1 MECHANICAL HOUSINGS Once installed the BCU CSU and the microscope form the protective housings of the product No components panels connections or linkages should be loosened or removed to avoid exposure to hazardous radiation 4 4 2 HAZARDS DUE TO MOISTURE OR LIQUIDS Please do not put components including power cables or external power supply in places with high moisture or near water oo a ee ere Version 2 0 rev 03 Mar 2015 26 ANDOR company OPERATION 4 5 USING THE BOREALIS 4 5 1 USEFUL INFORMATION After installation and the BCU is powered on there are no settings or adjustments required NOTE THE BCU SHOULD BE POWERED OFF WHEN NOT IN USE TO PRESERVE MOTOR LIFE 4 5 2 USING THE CSU DICHROIC SLIDER NOT APPLICABLE TO CSU VV If this has been specified select between the three dichroic positions using the selection knob as required e e 1 488 561 640 730 2 405 488 561 640 WARNING Ensure the CSU power is off when changing positions Slide the selection knob in out to select the required dichroic position you will feel a click for each position Wavelengths as selected at time of ordering are indicated on the label Figure 11 Selection of dichroic position 9 Version 2 0 rev 03 Mar 2015 27 ANDOR
26. ion 2 0 rev 03 Mar 2015 10 ANDOR RECOMMENDED RESPONSIBILITIES OF LASER SAFETY OFFICER These include but are not restricted to the following however national guidelines should also be referred to 1 Ensuring all personnel requiring access to the product are fully trained in both using the product and the general use of Class 3B and Class 4 lasers see below Ensure users are familiar with the hazardous properties of lasers namely that laser safety hazards differ from those of normal light radiation sources as they are high intensity highly collimated beams of electromagnetic radiation Ensuring the equipment is used in a controlled area by trained end users in accordance with national guidelines Ensuring end users are familiar with the operation of the laser s key switch control interlocks emission LEDs and other safety features Ensuring that all interlocks are connected and functioning correctly We recommend that a copy of IEC 60285 1 is purchased by the laser safety officer for reference SUIDELINES FOR SAFE OPERATION OF LASER PRODUCTS 1 2 3 Read the safety instructions supplied with all equipment in the system Never look into a laser beam either directly or indirectly Do not attempt to disassemble the unit housing the lasers or any part of the system If there is a problem please contact Andor directly see SECTION 1 1 Help and Technical Support Restrict and control ac
27. itioning Unit as per the International Laser Safety Standard IEC 60825 1 and the U S Laser Product Performance Standard 21 CFR 1040 10 As the Borealis system will form part of a unique configuration of components it cannot be classified in isolation Therefore when integrated it may form part of a system with one of the classifications described below A label in the format depicted below will be included for application by the System Integrator following assessment and classification The Borealis may be configured as part of a larger system that includes multiple Class 3B and Class 4 lasers A number of separate emission wavelengths can also be available in any one system In some very rare occassions another classification label may be used this will be explained in additional documentation as appropriate Class 1 Laser products that are safe during use including long term direct intrabeam viewing even when exposure occurs while using optical viewing instruments eye loupes or binoculars Class 1 also includes high power lasers that are fully enclosed so that no potentially hazardous radiation is accessible during use embedded laser product Intrabeam viewing of Class 1 laser products which emit visible radiant energy may still produce dazzling visual effects particularly in low ambient light Class 3B Laser products that are normally hazardous when intrabeam ocular exposure occurs i e within the NOHD including accidental short time
28. l fibre For transport or storage fibre cables should be coiled with a diameter of 300 mm or greater 23 Do not wet or spill liquids on the product and do not store or place liquids on the product 24 f spillage occurs on the product switch off power immediately and wipe off with a dry lint free cloth 25 If any ingress of liquids has occurred is suspected unplug the mains cables do not use and contact Andor Customer Support 26 Do not expose the product to extreme hot or cold temperatures 27 Do not expose the product to open flames 28 Do not allow objects to fall on the product 29 See SECTION 5 1 Cleaning and Decontamination 9 9 Version 2 0 rev 03 Mar 2015 4 ANDOR company LASER SAFETY CAUTION USE OF CONTROLS OR ADJUSTMENTS OR PERFORMANCE OF PROCEDURES OTHER THAN THOSE SPECIFIED HEREIN MAY RESULT IN HAZARDOUS RADIATION EXPOSURE This product is designed to be integrated with lasers that cover the entire visible soectrum and extend into the invisible infra red spectrum This section of the manual is designed to make the end user aware of the hazards of the product due to these lasers Laser safety hazards differ from those of normal light sources and users must be familiar with the hazardous properties of lasers as these are highly concentrated low divergence beams of radiation Andor recommend that all facili
29. m Equipment Information Model Serial No Check List Connections Emission LEDs S What is the nature of the problem Any other observations Version 2 0 rev 03 Mar 2015 30 TECHNICAL SPECIFICATIONS SECTION 7 TECHNICAL SPECIFICATIONS 7 1 BEAM CONDITIONING BCU FC APC Input power lt 10 W combined wavelengths 2m 7 2 BOREALIS ENHANCED CSU po 21 22 and CSU W Input wavelength range 400 750 nm 400 800 nm lt 500 mW combined wavelengths lt 500 mW combined wavelengths Uniformity for single dichroic systems 5 rms 10 rms 488nm using FRAPPA calibration slide Maximum frame rate fps 1000 FELD LENS CSU 10 21 22 Magnification options S short L long 1 0 S 0 9x L 1 5x S Tube lengths mm 37 6 109 3 187 1 4 5 ADAPTER Length range nominal mm 54 5 43 10 Tubes and bellows diameter mm 66 85 5 ENVIRONMENTAL 0C to 50 C lt 70 non condensing Overvoltage Category CAT Il An overvoltage category of CAT means that the equipment 15 designed to cope with transient voltages above the rated supply that would be experienced by any product connected to a mains socket in a building Rated Pollution Pollution Degree 2 Normally only non conductive pollution occurs Occasionally however
30. t http www andor com pdfs literature Andor_Standard_Warranty pdf Waste ELECTRONIC AND ELECTRICAL EQUIPMENT REGULATIONS 2006 WEEE The company s statement on the disposal of WEEE can be found in the Terms and Conditions E 9 9 Version 2 0 rev 03 Mar 2015 35
31. the CSU X This sealed unit permits safe and repeatable switching between the three user specified dichroic filters for additional flexibility in your experiments dichroics are specified by the user at time of ordering Note The CSU X should be powered off when changing positions 2 3 LASER SOURCES The Borealis is compatible with a wide variety of laser sources including the ILE and and the ALC 601 from Andor Refer to the information supplied by the manufacturer for each laser source Version 2 0 rev 03 Mar 2015 21 ANDOR company PRODUCT OVERVIEW 2 4 SYSTEM OVERVIEW The Borealis may be configured in a variety of ways An example set up 15 provided below Camera camera tube s E E E E E EE EE E SN Beam Conditioning Unit Borealis l erg Enhanced CSU X Armoured Fibre Optic 8 2 LASER LE i o m Fibre Optic 2 i MMF r Field lens tube I Bellows coupler l Microscope Figure 9 Overview of Borealis enhanced CSU X as part of an overall system Camera camera tube e g ALC 601 or ILE coupler Borealis Components A Fibre Optic MMF I Borealis Fibre Optic enhanced I MMF CSU W I i Conditioning Unit BCU Bellows i 1 I 1 4 Figure 10 Ov
32. ties have an established system for the safe use of lasers as per their national frameworks and Occupational Health and Safety legislation Laser Safety standards IEC 60285 1 and American National Standard Z136 1 2007 Safe use of Lasers may be useful references for good practice ASER PRODUCT CLASSIFICATION The Borealis system does not contain any embedded or external Laser Sources but it is intended to be integrated with a compatible Laser Source and has therefore been designed to safely accept and handle Laser Radiation The BCU and all ancillary components of the Borealis system have been designed and labelled to be compliant with IEC 60825 1 and CDRH Title 21 CFR 1040 10 The Borealis product includes an upgraded Yokogawa CSU spinning Disk Unit which has been classified and labelled as a Class 3B Laser Product in accordance with IEC 60825 1 and CDRH Title 21 1040 10 This Classification is not inherited by the Borealis Product as a whole as it may modify the specified laser wavelength and emission properties of the CSU device When assembled and integrated in the intended manner Borealis will form part of a unique Laser Product formed by the entire Confocal Microscopy System The Classification of this product will depend entirely on the Laser source connected and the individual combination of components in the Microscopy System Therefore the final assemblage must be assessed Classified and labelled in accordance with the requiremen
33. ts of IEC 60825 1 and CDRH Title 21 CFR 1040 10 This must be performed by a competent trained individual e g the site s Laser Safety Officer In the case of the System being installed by an Andor representative it is Andor s responsibility to assess and classify the final system The Customer Support Engineer will be competent and trained to perform this and ensure that the system is correctly classified and labelled Please refer to section 3 5 for guidance on the assessment and classification procedure Version 2 0 rev 03 Mar 2015 8 ANDOR company ASER SAFETY AND PRODUCT COMPLIANCE LABELS The label below is located on the rear panel of the BCU It indicates compliance with the applicable standards It also provides information on the manufacturer and identifies the model serial number and manufacturing date month year Spectral Applied Research Inc A Division of Andor Technology Model BCU NNN 9078 Leslie St Unit 11 Serial No 210000N Richmond Hill ON Canada 14 318 Manfactured 905 326 5040 www spectral ca This product complies with IEC 60825 1 2007 2 and 21 CFR 1040 10 and 1040 11 except for dems deviations pursuant to Laser Notice No 50 A dated 2007 06 24 40 12VDC2A Figure 1 Compliance and Serial Identification labels on rear panel of the BCU All relevant safety compliance information is visibly displayed on the Beam Cond
34. ture which is static and labelled Eye protection must be used by all Installation and service personnel when accessing any radiation during any installation or service procedure If eye protection is deemed desirable by the local Laser Safety Officer Andor recommends the following products e 360 nm 510 nm Kentek KXP 4001 Spectacles 510nm 670 nm Kentek KRA 6702 Spectacles VVORKING WITH OPTICAL FIBRES 1 Only service personnel authorized by Spectral or Andor should remove or inspect fibres 2 The laser radiation passing through fibres is potentially hazardous so great care should be taken to avoid damage that may lead to exposure to this hazardous radiation 3 fibre can be easily damaged by bending or general mishandling Ensure that the minimum curvature is never exceeded when handling Recommended minimum bend radius is 30 mm 4 Optical fibres are prone to damage by bending local to the connector 5 The coupler is not designed to withstand pulling of the fibre If the fibre is pulled the system performance could be compromised or the system may fail GH Version 2 0 rev 03 Mar 2015 12 ANDOR company INTRODUCTION SECTION 1 INTRODUCTION This manual provides an overview of the Borealis upgrade to Yokagawa CSU X CSU X1 or CSU W CSU W1 and its integration and function as part of a confocal spinning disk imaging system Borealis developed by Spectral provides a range of enhancements that enable yo
35. u get the best performance from your system e Increased uniformity of illumination e Increased throughput e Flexible bellows attachment to the microscope e Switchable field lens tubes Borealis Beam Conditioning Unit manufactured and CSU enhancements performed by SPECTRAL Spectral Applied Research Inc 2 East Beaver Creek Road Bldg 2 Richmond Hill ON CANADA L4B 2N3 Phone 1 905 326 5040 Email info spectral ca Web www spectral ca ANDOR an Oxford Instruments company Andor Technology An Oxford Instruments Company 7 Millennium Way Springvale Business Park Belfast 12 7AL Northern Ireland Web www andor com l 9 9 Version 2 0 rev 03 Mar 2015 13 ANDOR INTRODUCTION 1 1 TECHNICAL SUPPORT If you have any questions regarding the use of this equipment please contact the representative from whom your system was purchased or Europe USA Andor Technology Andor Technology Millennium Way 425 Sullivan Avenue opringvale Business Park Suite 3 Belfast South Windsor 12 7AL CT 06074 Northern Ireland USA Tel 44 0 28 9023 7126 Tel 1 860 290 9211 Fax 44 0 28 9031 0792 Fax 1 860 290 9566 www andor com contact_us support_request www andor com contact_us support_request Asia Pacific China Andor Technology Japan Andor Technology 4F NE Sarugakucho Building Room 1213 Building B 2 7 6 Sarugaku Cho Luo Ke Time Square Chiyoda Ku No 103 Huizhongli
Download Pdf Manuals
Related Search