UGenius-3-manual
Contents
1. Y Contents Page Contents Page Chapter OMeG INUOAUGHI OM oss ccdessscccassuctccsusecicnsacecivadbsbasviacsasteccatevicsateocesadevensiadaealaces 1 1 Applications supported EA HAWIG ane eee A A ae oe Ree eee ene 1 2 1 SDECI CANON soit a a olde 1 2 2 SYSTEM COMPONENTS ecen O eds 1 2 2 1 UV transilluminator 12 22 INET GRA sessar E R Ea E DEES 1 2 2 3 LCD Touch screen 1 2 2 4 USB Flash Drive 1 23 ACCESSOS a aaiantcladdatee dv otky aeniaeentds 1 2 3 1 Visible Light COMVEICN jeeactaa cue dosshodiccindasnn ateaiat an Gibansaunnisatencenthoiiands 1 2 3 2 Blue LED transilluminator 12 3 9 Lnermal Printer rarei e roe eiaaadadeedulies 1 2 3 4 Analysis software LZE Wee ee tee ne ete a gg a att tesa ta Chapter Two Image ACqQUISITION cccssseeeeeessseeeeeseeeseenseeeeenseseeeenneseeeeennees 2 1 Capturing an image of an agarose EtBr or similar Qel cccceesseeeeeneees 2 2 Capturing an image of a protein gel coomassie silver stained or other white light image colony plate autorad microtitre plate etc 04 Chapter Three Saving Opening and Printing IMages c sssessssseeeesees 3t SAVING IAG CS eenn a a a ane eenesees 3 2 Opening Images Side He PING TIACSS isis rack at aa ca echt ct as Chapter Four Image Enhanceme nt cccssseeccssseeeseeseescenseeseeenseseenneeeees 4 1 Image Enhancement 42 PIMOS nrar a E a Seeds aaciusdetent A3 Digital Zoo Maen a a a Chapter Five Use2. switch Step Two Manually adjust the camera settings such as the aperture zoom and focus Adjust the aperture zoom and focus settings until a suitable image is displayed preferably with the sample filling the screen for maximum resolution Please note that opening the aperture too far may result in areas of the sample being saturated Figure 6 Lens control Step Three The exposure time can either be set manually or automatically Adjusting the exposure time will alter the brightness of the image To manually increase or decrease the exposure time use the following icons Figure 7 Manual Exposure controls U Genius User manual 7 2 Image Acquisition The auto exposure function sets the exposure time to a level that ensures no saturation of the image occurs To use the auto exposure function select the following icon Figure 8 Auto Expose icon Step Four To capture the image press the Capture button If you have selected auto expose then you will not need to press the Capture button Figure 9 Capture button U Genius User manual 8 3 Saving Opening and Printing Images Chapter Three Saving Opening and Printing Images 3 1 Saving images To save a captured image press the Save button N B You can only save an image to a USB device or your local area network LAN Images can be saved in the following formats TIFF and JPEG Figure 10 Save Button 3 2 Opening Images From the fron
3. 17 Preferences screen U Genius User manual 13 5 User Preferences 5 1 Neutral fielding The neutral field function is based on powerful algorithms which corrects for uneven illumination This results in an image with a flat even background whilst maintaining GLP compliance To neutral field check the box Figure 18 then follow the on screen instructions Figure 19 m 5 i NEUTRAL FIELD 4 Figure 18 Neutral fielding button Neutral Fielding i l Please remove your sample and then EITHER Place the neutral field screens on the transilluminator OR use the white light screen pad OR use the NovaGlo screen as appropriate AND then press OK Figure 19 Neutral fielding on screen instructions 5 2 Extended Dynamic Range EDR Many gels contain areas that are too bright or too dark to be successfully captured Closing the iris to cut down the light may prevent the viewing of dark or faint bands whilst opening it may lead to saturation EDR solves this problem by automatically capturing a series of images of different exposure times The content of each image is assessed and those areas that are within the dynamic range of the camera are combined The result is an image with a large dynamic range up to 65536 grey levels that encompasses all the brightness ranges existing within the sample without saturation Figure 20 EDR button U Genius User manual 14 5 User Preferences 5 3 External Keyboard If you wi
4. pixels Image storage Rotation and inversion sharpen smooth and many more ee Slide in and out UV transilluminator visible light converter and blue LED transilluminator Ultra Slim Epi white LED U Genius User manual 1 1 Introduction 1 2 2 System Components CAMERA bhai Phe eS eh ee Ee EEEE T a a eS a E E i h a 7 b E la F k Superb 3 million pixel resolution LENS LEEELEELTEELEETEELEETEELEETEETEE TEET E T Superbr zoom lens for exceptional image quality FILTER DRAWER Liss arange of filters for extensive choice of apolications SSPE PEE Pee a a h Pr Ee INTEGRAL COMPUTER High specification integral computer featuring embecdcect Windows XP operating system network USB ports hard grive TOUCH SCREEN Large oolour LOD screen USB Stores images on a USB memory stick SAFETY SWITCH eeaanoanes oad Protects from accidental LW exposure when opening door INTERNAL WHITE LIGHT Prererr try tet te For sample positioning and focusing TRANSILLUMINATORS OPTIONAL ee a ee For WY or Glue light UWY transilluminator slices in and out of darkroom Blue LED light UttraSlim LED sits on a slice in ancl out tray Visible light converter SLIDING DOOR Space saving sliding coor see the ondineg Syngene database for detalls 1 2 2 1 Darkroom The darkroom has a sliding door The darkroom features e Slide out mid wave 302nm UV t
5. the aperture zoom and focus Adjust the aperture zoom and focus settings until a suitable image is displayed preferably with the sample filling the screen for maximum resolution Please note that opening the aperture too far may result in areas of the sample being saturated Aperture Zoom Focus Figure 2 Lens control U Genius User manual 5 2 Image Acquisition Step Three The exposure time can either be set manually or automatically Adjusting the exposure time will alter the brightness of the image To manually increase or decrease the exposure time use the following icons Figure 3 Manual Exposure controls The auto exposure function sets the exposure time to a level that ensures no saturation of the image occurs To use the auto exposure function select the following icon Figure 4 Auto Expose icon Step Four To capture the image press the Capture button If you have selected auto expose then you will not need to press the Capture button Figure 5 Capture button U Genius User manual 6 2 Image Acquisition 2 2 Capturing an image of a protein gel Coomassie silver stained or other white light image Colony plate autorad microtitre plate etc Step One Make sure that you place the visible light converter on top of the transilluminator Position your sample on the centre of the visible light converter and close the door Manually turn on UV by pressing the UV On
6. UPD897 When you first attach the printer you will see the following window Poul Sew Hardware Wierd Welcome to the Found New Hardware Wizard Wingo vall seach lor cument and updated salvare by lnckung on yow computer on tre hademe installahon CD or on hee windo Update tahab siba nah pou permitto Resd ou pracy palce an Windows connect bo Windows Lpdale to seanch lov otraa C Yes this ime oniy C Ye now and ever lee coined a devine C Mo mot this fime Likt Het ic conim Select No not this time and press Next button The drivers will automatically install 1 2 3 4 Analysis software The U Genius system is supplied with a copy of GeneTools analysis software from Syngene This may be loaded on a PC of your choice Please see the loading instructions within the CD cover 1 2 4 Set up Please refer to the Installation quick guide on how to set up the camera and darkroom U Genius User manual 4 2 Image Acquisition Chapter Two Image Acquisition 2 1 Capturing an image of an agarose EtBr or similar gel For rapid capture see Quick Guide Capture Step One Position the sample on the centre of the transilluminator close the door ensure the UV filter has been placed in the filter draw and then manually turn UV on by pressing the UV On switch on the front of the unit Then press the Live button icon Figure 1 LIVE view icon Step Two Manually adjust the camera settings such as
7. ion functions select the Annotate button via the Edit Menu button From this menu you can add text change the font select to have your text horizontal or vertical or remove text Use this icon to add text to your image Press or place the mouse cursor on your image and type your text To change the font style press this icon lf you wish to have your text displayed horizontally select this button L HORIZONTAL TEXT If you wish to have your text displayed vertically select x this button VERTICAL To remove any annotation select this button Use the Back button to navigate between menus U Genius User manual 11 4 Image Enhancement 4 3 Digital Zoom To zoom in or out on your image select the Zoom button from the screen once you have captured your image Figure 15 Zoom button Digitally zoom in on your image to see faint bands Digitally zoom out on your sample Zoom settings can reset at any time Use the Back button to navigate between the menus U Genius User manual 12 5 User Preferences Chapter Five User Preferences To access U Genius settings and set user preferences for neutral fielding EDR to view external keyboard and to set regional power frequency settings PREFERENCES Figure 16 Preferences icon You will be directed to a new screen Figure 17 Frozen 0 100s Remember to turn the UV off MN a NEUTRAL FIELD EXTERNAL KEYBOARD Figure
8. r Preferencess ccccccssseeccesseeceesseesensseseeeneeeseenseesenseees Si Neutral Fielding enose e Ea 5 2 Extended Dynamic Range EDR Bid EXlemal Keyboard se A 5 4 Regional Power Frequency SettindS ccccccccssseeeeeeeseeeeeseeeseeeesaeeeeeeeaas Chapter Six Troubleshooting and Contact Information csecessseee OP TOUDIESNOOINO cesen tentacles beeen a tatisdattacedsonaceatesstactes 6 2 Contact Syngene U Genius User manual 1 1 Introduction Chapter One Introduction The U Genius system has been designed to make your gel imaging simple quick and easy This system is the perfect choice for a low budget gel imaging system and features a compact darkroom which has a sliding front door and internal LED white light The U Genius system supports multiple applications including DNA RNA gel imaging such as EtBr SYBR Green and visible stained gels i e coomassie blue and silver stain 1 1 Applications supported UV Converter Transilluminator Ethidium Bromide Coomassie blue stain SYBR Gold SYBR Green Copper stain SYBR Green SYBR Gold Zinc stain SYBR Safe SYBR Safe Silver stain UltraSafe blue Gel Green Gel Red SYPRO Ruby 1 2 Hardware 1 2 1 Specification 12 16 bit 1 3 inch Manual zoom 6 5 39 F1 4 aperture and focus Display ss Display 8 colour touch screen Filter Draw Yes UV filter as standard 20 x 20 cm Data types TIFF and JPEG CCD resolution 3M
9. ransilluminator e Internal white light LED e Safety switch to protect from accidental UV exposure U Genius User manual 2 1 Introduction 1 2 2 3 UV Transilluminator The UV transilluminator will excite many fluorescent stains such as Ethidium bromide SYBR stains Gel Red The standard wavelength is 302nm To protect users from accidental exposure the UV light is automatically shut off if the door is opened The transilluminator can be slid easily in and out of the cabinet 1 2 2 4 Filter Drawer Interchange a range of filters for extensive choice of fluorescent applications 1 2 2 5 LCD Touch Screen A built in 8 inch VGA colour LCD touch screen allows users to preview capture print and save images as well as to select various processing functions without the connection of an external keyboard or mouse 1 2 2 6 USB Flash Drive The USB flash drive on the front of the U Genius for the easy storage of images 1 2 3 Accessories 1 2 3 1 Visible light converter syngene offers a visible light converter that can be placed on top of the UV transilluminator for imaging coomassie and silver stained gels 1 2 3 2 Blue LED transilluminator The UltraSlim blue LED transilluminator is ideal for safely imaging gels stained with SYBR Safe Gold and Green GelGreen and UltraSafe blue U Genius User manual 3 1 Introduction 1 2 3 3 Thermal printer You may attach a thermal printer either Mitsubishi P93D or Sony
10. sh to connect an external keyboard to the U Genius system select this button SRGRE 22 a P EXTERNAL KEYBOARD Figure 21 External Keyboard button 5 4 Regional Power Frequency Settings Select your regional power frequency settings choose between 50Hz or 60Hz Figure 22 Regional Power Frequency Settings buttons U Genius User manual 15 6 Troubleshooting and Contact Information Chapter Six Troubleshooting and Contact Information 6 1 Troubleshooting No power to the darkroom e Check connection of main power cord to main power port on the rear of the U Genius e Try another power socket within lab Transilluminator will not turn on e Check power cord by sliding transilluminator fully out from cabinet If loose push back in e f still not on remove power cord and attach another one plugged in elsewhere If transilluminator comes on there is an electrical supply problem within the U Genius If it still does not come on it is likely the transilluminator has failed Contact Syngene 6 2 Contact Syngene EUROPE USA BEACON HOUSE 5108 PEGASUS COURT SUITE M NUFFIELD ROAD FREDERICK CAMBRIDGE MD 21704 CB4 1TF Tel 44 1223 727123 Tel 800 686 4407 1 301 662 2863 Fax 44 1223 727101 Fax 301 631 3977 Email sales syngene com Email ussales syngene com www syngene com U Genius User manual 16
11. t screen select Open Image to browse captured images Figure 11 Open Images Icon 3 3 Printing Images If you have a thermal printer attached to your U Genius press the following button Figure 12 Print button U Genius User manual 9 4 Image Enhancement Chapter Four Image Enhancement The U Genius system offers a variety of functions ranging from image enhancement to annotation 4 1 Image enhancement To access the enhance functions select the Enhance button via the Edit Menu button From this menu you can select to smooth sharpen invert or rotate your image Figure 13 Enhance icon INVERT Click on this icon to apply a smoothing filter This is useful when the image has specks of dust or bubbles present However this will make bands appear less sharp Click on this icon to apply a sharpening filter to your image When this filter has been applied band edges should become more pronounced but you may also observe an increase in the graininess of the image The Invert icon will reverse the image to give black bands on a white background or vice versa This icon is particularly useful when trying to see faint bands To freely rotate your images press the Rotate icon Press the Undo button to remove any enhancement that you may have performed Use the Back button to navigate between the menus U Genius User manual 10 4 Image Enhancement 4 2 Annotate To access the annotat
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