Ettan IPGphor 3 Control Software
Contents
1. Help IPGphor help Ele protocol Communication paip C EE CA tetra or rat Pade Tee slalla 3 Ettan IPGphor 3 Rian setings amp details I Session log l Strip length OH range Number of strips 10000 200 1 Instrumenti 30 po a0 wo 700 W Step 0 0 Measured Setpoint g 0 12 7 ov sao 100 Opa 3 ns ai 0 Vh JIA w z0 ion 00 00 00 w s mo mo mm oo ww oso ORO ono W 00 Te Measured voltage w Setpoint voltage v wf Total time Total Vh Fig 3 1 Ettan IPGphor 3 Control Software application window with Run settings amp details tab and Info and data view opened Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 11 Software overview 12 3 2 Menus To view the drop down menu options click on the selected header or use keyboard commands indicated by underlining Alt underlined header letter underlined option letter or ctrl letter Header Option Short Function Bler ee command Export Page setup Print Ctrl P Preferences Quit Ctrl Q Protocol New Ctrl N Open Ctrl O Save Ctrl S Transfer protocol Upload all Download all Communication Instrument 1 Instrument 2 Instrument 3 Instrument 4 Help IPGphor help Ctrl H About IPGphor 3 software Exports all log data to MS Excel format Specifies printing settings Prints the display the current protocol or the session log Specifies the export and prot2. The maximum voltage of 10000 V should only be applied to IPG strips of 18 and 24 cm run in Ettan IPGphor Cup Loading Manifold and the Manifold Light Other IPG strip holders have a maximum voltage of 8000 V Regarding IPG strips of other lengths than 18 and 24 cm please see the attached instructions of Immobiline DryStrip package Depending on the pA limit of the isoelectric focusing run the conductivity of the IPG strip rehydration solution and the sample Ettan IPGphor 3 isoelectric focusing Unit may not operate at the set maximum voltage Time VHrs Each step of the protocol can be programmed to run for a specified Time or for a specific number of VHrs volthours Volthours is the product of the voltage multiplied by the number of hours for each step The maximum value for Time is 99 hours and 59 minutes Each VHrs step may be set from 1 99 999 VHTrs If more than 100 000 VHrs is desired it will have to be split between two steps At least 1 minute or 1 VHr must be programmed for a step or the program will end at that step regardless of what values are set for any subsequent steps Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Software overview E 3 4 5 Protocol details graph To get an overview of the protocol and for monitoring the run two of three possible parameters setpoint voltage measured voltage and measured current can be displayed as graphs Ettan IPGphor 3 Control Software graphs the data
3. sai ccdesssasssssscccccevassssesvssnssscsconcesseasescnsansenaccoszsstscstestes 24 Setting protocols 5 1 Using the Fast Mode protocol option woceeccccccccsssssssseesssssccsssssssssseeees 25 5 2 Using the Advanced mode protocol Option uu 26 5 2 1 Creating MEW protocols oacceeecesessssssssssssssssssssssssssnssssssscssscsssseeseseessessssssssssess 26 5 2 2 Opening EXISTING protocols ceesssssssssssssssssssssssssssscssesessessseseessssessesssssssesssas 27 5 2 3 Editing Protoc l Sarane annain a E 28 5 2 4 Saving protocols amp 5 2 5 Downloading protocols to instrument at specific positions 29 5 2 6 Deleting PrOtO COIS este attics ate E E ETTE 29 5 3 Printing Protocols essere ERE AA RREA 30 Starting and monitoring runs Gel Starting TUNS yenii a eE EE REE EE 31 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB iii 6 2 PO USING TUMS Ainiin Aa E 31 6 3 Stopping runs 31 6 4 MONItON GE GUIS 4 deeoscscacecd piiccaasccacSeticzastacccessssscsestaseasestasavssssnscaadteccuesecsonaseaeies 32 Handling run data and log files 7 1 Exporting run data and log files ieecccccssssssssseesssssccssssssssssnsesessseesessssssees 35 7 2 Printing run data ANd log files eececccsssssssssseseesssscccccssssssssseeeesssseesessen 36 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Introduction er 1 Introduction 1 1 Introduction Ettan IPGphor 3 Control Software is enclosed when purchasing IP
4. 16 2424 Finland amp Baltics Tel 358 0 9 512 39 40 Fax 358 0 9 512 39 439 e France Tel 01 69 35 67 00 Fax 01 69 41 96 77 Germany Tel 0761 4903 490 Fax 0761 4903 405 e Italy Tel 02 27322 1 Fax 02 27302 212 e Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 Latin America Tel 55 11 3933 7300 Fax 55 11 3933 7304 e Middle East amp Africa Tel 30 210 9600 687 Fax 30 210 9600 693 Netherlands Tel 0165 580 410 Fax 0165 580 401 Norway Tel 815 65 555 Fax 815 65 666 e Portugal Tel 21 417 7035 Fax 21 417 3184 e Russia amp other C I S amp N I S Tel 7 095 232 0250 956 1137 Fax 7 095 230 6377 South East Asia Tel 60 3 8024 2080 Fax 60 3 8024 2090 e Spain Tel 93 594 49 50 Fax 93 594 49 55 e Sweden Tel 018 612 1900 Fax 018 612 1910 Switzerland Tel 0848 8028 12 Fax 0848 8028 13 UK Tel 0800 616928 Fax 0800 616927 e USA Tel 800 526 3593 Fax 877 295 8102 imagination at work User Manual 11 0034 59 AB
5. indicates which of Vh and Time that is selected to define the length of the current step 1 Alpha Step 1 4 Measured Setpoint 300 300 V Opa 50 pA limit 9 Vh 00 01 39 02 41 estimated Total time Total h 02 42 5750 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 33 6 Starting and monitoring runs E 6 7 Open the Session log tab by clicking the tab heading to view the running data collected in the log file Run settings amp details Session log Session log Ettan IPGphor 3 Ej Serial Number 4711 Firmware version AZ 02 20 04 Date May 17 2005 09 16 User andreas trende logged on 09 16 Protocol GE_18_5 0 6 0 No of strips 8 IEF Params 40uA Strip at Z0 C Step 1 Step 500V 500 Vhr Step Z Grad 1O00V 800 Vhr Step 3 Grad 1lO000V16500 Vhr Step 4 Step 10000V39200 Vhr 09 16 Step 1 started 10 15 Step 2 started Click another instrument control button for monitoring runs on other instruments controlled from the software This way it is possible to monitor up to four parallel runs 34 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Handling run data and log files 7 Handling run data and log files After a run is finished or stopped see section 6 3 there is an option to save recorded running data and log file for future reference 7 1 Exporting run data and log files 1 Click on the desired instrument button if not alr
6. GE Healthcare Ettan IPGphor 3 Control Software User Manual Ettan Contents Introduction 1 1 troduction A aia 7 1 2 Associated documentation sss ssssssrsssssrssssseressserrssseeerssseeressseorssseerasssenes 8 Requirements and installation 2 1 Computer requirements ou eeecssssssescsssseecsssssecccssssccesssecsessseccesssecessseecessseesees 9 Z2 Software STILL sesssccscscststs nccsceczesscconssccanssbetcoccectentendonsdetnennnsaoddestosensienSins 9 Software overview S1 Application WIMAOW esiisa a 11 3 2 MERUS citar ctecatiats 3 3 Control buttons a 3 3 1 INStHUMENESCLECHON ics tssncissdiansdinactianninianananaenanits 13 3 3 2 Protocol Modesoessun ennan e RENN 13 3 3 3 Instrument control wd 3 4 Run settings amp details tab oo cccccccsssssssssseeeesessseesen 14 3 4 1 ComboBoxes for entering experimental conditions 14 3 4 2 Advanced mode protocol HANIING sssssssssssssssssssssssssssssssssssssssssessessseeeeeees 15 3 4 3 Protocol VIG Wai Grenteng naa RANN 16 3 4 4 Range of parameters allowed 3 4 5 Protocol details graph 3 4 6 Info and data view as 3 5 Session logta Buinian kih 21 Starting the software and connecting instruments AL QMEMING the SOPWONE s cssrsecssosssssssccesconnsnvsssaie4 5sssescestansauvadsseaassssssccedsssvastes 23 4 2 Connecting INSTFUMENHS ooa eeescssssesccsssesccessseccssssccsssssecsssssecssssseccessseesssuseess 23 43 Closing th SOPWOE
7. Gphor 3 instrument order number 11 0036 23 User friendly Ettan IPGphor 3 Control Software is a user friendly interface to the Ettan IPGphor 3 isoelectric focusing Instrument as well as it provides an enhanced functionality in the areas of data presentation data storage and protocol handling Tool tips will appear as the mouse is moved across active buttons to guide the user in navigating and using the software Online help for several of these functions is obtained using the menu Help IPGphor help Protocol selection Using Fast mode settings recommended standard GE protocols are available to each strip length and pH interval Using Advanced mode settings new protocols are created All protocols can be edited and saved for later use The selected protocol is loaded to an Ettan IPGphor 3 isoelectric focusing Instrument Monitoring and documentation Ettan IPGphor 3 Control Software provides excellent possibilities for monitoring and documentation e While isoelectric focusing of Immobiline DryStrip gels progresses the software records voltage and current and graphs them as the run proceeds over time In addition a log file is created e Whenarun is completed graph and log file can be printed and or exported to a Microsoft Excel format Multiple instrument control Ettan IPGphor 3 Control Software can be used to control up to four Ettan IPGphor 3 units at one time each running different protocols and sets of run paramete
8. Protocol view 16 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Software overview was Item Description Protocol name Shows current protocol name Rehydr time Sets strip rehydration time Rehydr Temp Sets temperature of IPGphor 3 platform during rehydration IEF I strip uA Sets maximal electrical current during isoelectric focusing per strip IEF Temp Sets temperature of IPGphor 3 platform during isoelectric focusing Step 1 9 Makes it possible to set conditions for up to nine different steps during isoelectric focusing Select Step or Grad gradient for each step The duration of each step is defined by selecting either Time or Volthours Vh Total time Summarizes total running time for the protocol not taking into account effects of reduced voltage due to isoelectric focusing current limits Total Vh Summarizes total volthours for the protocol not taking into account effects of reduced voltage due to isoelectric focusing current limits 3 4 4 Range of parameters allowed Protocol name The protocol name can contain up to 16 characters Note The naming nomenclature of the GE protocols selected by the software in the Fast protoco mode follows the principle The name is coupled to the length of the IPG strip and the pH range that is being run Rehydration time Using the Strip Holder the strip is rehydrated with the sample directly in the IPGphor 3 Rehydration and separation are then pe
9. Voltage and Current as it is retrieved from Ettan IPGphor 3 isoelectric focusing Unit Protocol details 10000 200 9000 180 8000 160 7000 140 6000 a 5000 100 Voltage Y Current uA 4000 80 3000 60 2000 40 a i i i i i i i 1 i 1 1 O oo 50 01 40 02 30 03 20 04 10 05 00 05 50 06 40 07 30 08 20 08 59 Time Measured voltage Y wW Setpoint voltage Y wW Fig 3 7 Protocol details graph with Measured voltage and Setpoint voltage selected Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 19 Software overview 20 3 4 6 Info and data view The info and data view is used when monitoring a run The following items are displayed e Selected instrument identity e Current protocol step e Measured and Setpoint voltage and current e Accumulated volthours Vh An underscored value indicates that the current step has a set Vh volthours value e Elapsed time An underscored value indicates that the current step has a set Time value e Estimated time left experimental time can diverge from Total time due to e g disturbances in the electric current e Total time the theoretical running time of the protocol e Total Vh volthours set in the protocol e Buttons for selection of Info and Data view and Protocol view 1 Alpha Step 1 4 Measured Setpoint 300 300 V Opa 50 pA
10. b window Run settings amp details Session log Strip length pH range Number of strips 6 The software now selects an optimized GE protocol for the specified experimental parameters Optional It is possible to edit the current Fast mode protocol Select Advanced mode by clicking the right of the two central control buttons and see 5 2 3 for further instructions Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 25 Ee Setting protocols E 5 2 Using the Advanced mode protocol option Open the software as described in section 4 1 Click on an Instrument button the four buttons to the top left of the main window to select the desired instrument Select Advanced mode by clicking the right of the two central control buttons Advanced should be shown below the buttons Select Run settings amp details tab by clicking the tab head if it is not already open Set Number of strips by using the ComboBox at the top of the tab window Now several options is possible It is possible to create a new protocol to open edit and save protocol to and from protocol folders and to delete an existing protocol 5 2 1 Creating new protocols 1 2 3 Fast Adv Advanced 4 Number of strips 2 Za 5 1 2 3 TE 26 Open a new protocol by clicking the left of the three buttons or by using the main menu Protocol New Ctrl N Run settings amp details Session log Protocol file Numb
11. e This command also disconnects any connected instruments Note Ifthe instrument is in run mode the connection to the instruments will be lost and no run data or log files can be saved for the current runs 24 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Setting protocols 5 5 Setting protocols When setting the protocol for an isoelectric focusing run in the Ettan IPGphor 3 Control Software there are two major options Fast mode and Advanced mode In the Fast mode option the software selects an optimized protocol based on what manifold type strip length and pH range to be used After the software has selected a protocol it is optional to edit it Inthe Advanced mode option there are many possibilities for setting the protocol It is possible to start with a new empty protocol to use an existing protocol or to open and edit an existing protocol 5 1 Using the Fast mode protocol option 1 Open the software as described in section 4 1 2 Click on an Instrument button the four buttons to the top left of the main window to select the desired instrument 3 Select Fast mode by clicking the left of the two central control buttons Fast ase cy should be shown below the buttons Fast 4 Select the Run settings amp details tab by clicking the tab head if it is not already open 5 Set experimental conditions that is Strip length pH range and Number of strips by using the ComboBoxes at the top of the ta
12. eady done to select the desired run 2 Export running data and log file of the current run by using the menu File Export 3 Select the desired Export options by clicking in the tick boxes Click OK to export the data The current screen can be saved as a jpg image and the run data in a Microsoft Excel file o Enporta Export options IV Save screen to jpg file JV Save run data to MS Excel file Cancel 4 The selected export options are now saved in a default export directory Note To change the default folder for your export directory use the menu File Preferences and browse for the desired Export directory folder and then click OK x Export directory A C IPGphor3 RunData Protocol directory E C IPGphor3 Protacals 5 Edit the export file default names if desired The default names are generated by date and time e g Apr 20 2005_ 14 21 20 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 35 Handling run data and log files EEE ints 7 2 Printing run data and log files 1 Select the menu File Print Ctrl P 2 Select the desired Print options by clicking in the tick boxes and then click OK x Print options Iv Display screen IV Current protocol IV Session log Lad 36 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB www gehealthcare com GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden Ettan Immobiline and IPGphor a
13. er of strips SBE esl Now empty fields for different parameters is shown in the Protocol view To enter data click with the cursor in a field and write the desired text or values There are switch buttons for choice of Step Grad gradient for U V voltage and HH mm Vh volthours for Time or Vh for each protocol step See 3 4 4 for allowed limits for each parameter Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB a 5 2 2 1 Setting protocols 5 Opening existing protocols Select the Protocol view in the Run settings amp details tab by clicking the right of the two buttons at the low right corner of the tab window Click the Protocol file ComboBox and select the desired protocol in the list of protocols Run settings amp details Session log Protocol file Number of strips SBE aol If the desired protocol is not present in the default folder open the menu Protocol Open Ctrl O and browse for the folder containing the desired protocol The set values of the protocol is now shown Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 27 Ee Setting protocols 28 5 2 3 Editing protocols 1 Select the Protocol view in the Run settings amp details tab by clicking the right of the two buttons at the low right corner of the tab window Click the Protocol file ComboBox and click on the wanted protocol in the list of protocols If the desired protocol is
14. g and monitoring runs 6 6 Starting and monitoring runs 6 1 Starting runs 1 Select instrument by clicking the desired Instrument button 2 Select a protocol to run see 5 3 Select the number of strips to run see 5 4 Click the Start button the left button of the three instrument control buttons The current protocol is now downloaded to position 1 in the selected instrument The run is now started which is indicated by the word Running below the instrument control buttons Running 6 Tostart a second run select the desired instrument protocol and number of strips to run and click the Start button It is possible to start up to four different runs using four different protocols on four different instruments in parallel 6 2 Pausing runs 1 Click the Pause button the middle button of the three instrument control buttons The run is now paused which is indicted by the word Paused below the instrument control buttons Paused 2 Click the Start button to proceed the run The run is started from the same position in the protocol from where it was paused 6 3 Stopping runs 1 Click the Stop button the right button of the three instrument control buttons to stop an ongoing run The run is now stopped which is indicted by the word Idle below the instrument control buttons Idle Note When using the stop button it is not possible to resume the run again However it is possible to start from the beginning of the
15. limit 9 Vh 00 01 39 02 41 estimated Total time Total h 02 42 5750 Fig 3 8 Info and data view Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Software overview g 35 Session log tab In the Session log tab selected parameters and data retrieved from Ettan IPGphor 3 isoelectric focusing Unit from the run are collected The instrument log records data once a run has started Examples of session log data are e Instrument ID e Date e Log on and log off times e Run starting and ending times e Step starting and ending times e Run pausing time Run settings amp details Session log Session log Ettan IPGphor 3 EJ Serial Number 4711 Firmware version AZ 02 20 04 Date May 17 Z005 09 16 User andreas trende logged on 09 16 Protocol GE_18_5 0 6 0 No of strips 8 IEF Params 40uA Strip at ZO C Step l Step 500V 500 Vhr Step Z Grad 10000 800 Vhr Step 3 Grad 10000V16500 Vhr Step 4 Step 1lO000V39Z00 Vhr 09 16 Step 1 started 10 15 Step Z started Fig 3 9 Session log tab Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 21 Software overview 22 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Starting the software and connecting instruments 4 4 Starting the software and connecting instruments 4 1 Opening the software Click Programs in the Windows Start menu and select the Ettan IPGphor 3 Con
16. not present in the default folder open the menu Protocol Open Ctrl 0 and browse for the folder containing the desired protocol This folder will now be the new default folder from which the Protocol file ComboBox is populated Run settings amp details Session log Protocol file Number of strips BEES at The set values of the protocol is now shown To edit the opened protocol click in a field and write the desired text or values There are switch buttons for instrument protocol position and choice of stepping gradient of voltage and time volthour for each protocol step See section 3 4 4 for allowed limits for each parameter Protocol name AT_18_5 0 6 0 Rehydr time 00 00 Temp 20 C IEF Ifstrip 75 m Temp 20 C Step U Time or h 1 Step x 500 wu 500 Vh 2 Grad 1000 wm s00 vh f 3 Grad 10000 s 16500 vh wl 4 Step wh 10000 39200 vh 5 Stee who mu 00 00 HH mm 6 step fo ww 90 00 HHimm lt 7 Step x 0 u 00 00 HHimm 8 Step xi 0 u 00 00 HHimm gt 9 Step oF 0 m 00 00 HHimm Total time Total h 08 59 57000 BE Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Setting protocols 5 E 524 Saving protocols 1 Check that the desired protocol name is entered in the Protocol name box A tip is to name the protocol according to user name_strip length_pH range upper limit 16 characters 2 Click the middle of the three buttons in order to save the p
17. ns When a green lamp on a instrument selection button is lit the corresponding instrument is connected to the software see Fig 3 2 instrument 1 3 3 2 Protocol mode With the mid group of buttons Fast mode or Advanced mode for setting protocol is selected for runs on Ettan IPGphor 3 isoelectric focusing units e With Fast mode the software selects an optimized protocol for the current experimental conditions e With Advanced mode it is possible to choose and edit any existing protocols or to make an entirely new protocol to optimize experimental performance in different aspects The current selected mode is shown by shading of the appropriate button and by text below the buttons Note It is possible to let the software select a protocol using the Fast mode and then edit the protocol in Advanced mode before downloading to an instrument 3 3 3 Instrument control With the right group of buttons the run of the selected instrument is controlled by Start Pause and Stop control buttons The selected state of the run is indicated by shading of the appropriate button and by text below the buttons Note Ifthe stop button is used the run can not be resumed Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 13 Software overview 14 3 4 Run settings amp details tab This tab is used when defining experimental parameters setting protocols downloading protocols to the instruments and when monitoring an ongoing r
18. ocol directories Disconnects from the instrument and closes the program Starts a new protocol Opens a protocol The last opened folder will be set as default folder Saves the current protocol The last opened folder will be set as default folder Downloads the selected protocol to the selected instrument at desired position 1 10 Use at instrument service Uploads all proto cols from an instrument to a file on the computer Use at instrument service Downloads the previously uploaded protocols to an instrument Opens a communication properties dialog for communication gate specification instrument designation and instrument connection See above Function for Instrument 1 See above Function for Instrument 1 See above Function for Instrument 1 Opens the Online Help Opens the About dialog Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Software overview 3 3 Control buttons Three groups of control buttons are located in the top of the application window Instr Instr2 1 Instr3 1 Instr4 mi rast aav f miii 1 Alpha Fast Running Fig 3 2 The three groups of control buttons of the Ettan IPGphor 3 Control Software appli cation window 3 3 1 Instrument selection With the left group of buttons one instrument is selected for downloading a protocol starting a run or when monitoring ongoing runs The selected instrument ID is shown by shading the chosen button and by text below the butto
19. protocol Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 31 6 Starting and monitoring runs 64 Monitoring runs 1 Click the correct Instrument control button to select the desired instrument The identity of the selected instrument is shown below the control buttons 2 Open the Run settings amp details tab by clicking the tab heading if not already open 3 Inthe Protocol details graph two of three possible parameters setpoint voltage measured voltage and measured current are displayed to give an overview of the protocol and the run Protocol details 10000 200 9000 180 8000 160 7000 140 6000 120 5000 100 Voltage v Current A 4000 80 3000 60 2000 40 20 1 1 I 1 1 1 j 1 1 j 00 50 01 40 02 30 03 20 04 10 05 00 05 50 06 40 07 30 08 20 08 59 Time Measured voltage Y v Setpoint voltage Y v 4 Open any of the ComboBoxes below the graph and then click on the desired parameter to be displayed in the Protocol details graph Note If measured voltage and measured current are selected the time scale changes and only the elapsed time is shown 32 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Starting and monitoring runs 6 E 5 Click the left of the two buttons at the lower right corner of the tab window to select the Info and data view The main running parameters are now displayed The underlined parameter
20. r delete the current protocol Run settings amp details Session log Protocol file Number of strips m GIES nnl Fig 3 5 ComboBoxes and buttons in advanced mode protocol selection With the Protocol file ComboBox an existing protocol from the default protocol folder is selected To change default folder use the menu File Preferences There are three buttons to the right of the Protocol file ComboBox e New protocol button left with which a new protocol is created e Save protocol button middle with which the current protocol is saved in the default protocol directory folder e Delete protocol button right with which the current protocol is deleted from the hard disc Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 15 Software overview E 3 4 3 Protocol view The protocol view is used when creating a new protocol or for editing an existing protocol Values in fields can be edited Voltage Step Grad mode and Time Vh mode can be selected for each Step Protocol name AT_18_5 0 6 0 Rehydr time 00 00 Temp 20 C IEF Ifstrip 75 Temp 20 oC Step u Time or h Step 500 wn 500 vh Grad 1000 800 Vb Grad 10000 16500 Vb gt 4 Step W 10000 39200 Step yio uu 00 00 HHimm Step W80 uu 00 00 HH mm V Sten gjo uu 00 00 HH mm Step 740 we 00 00 HHimm rennet a UN Step TO wu 00 00 HHimm gt Total time Total h 08 59 57000 Fig 3 6
21. re trademarks of GE Healthcare Ltd GE tagline and GE monogram are trademarks of General Electric Company Windows is a trademark of Microsoft Corporation All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them GE Healthcare reserves the right subject to any regulatory and contractual approval if required to make changes in specifications and features shown herein or discontinue the product described at any time without notice or obligation Contact your local GE Healthcare representative for the most current information 2005 General Electric Company All rights reserved GE Healthcare Bio Sciences AB a General Electric company GE Healthcare Bio Sciences AB Bjorkgatan 30 751 84 Uppsala Sweden GE Healthcare Munzinger Strasse 9 D 79111 Freiburg Germany GE Healthcare Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Paci c Tel 852 2811 8693 Fax 852 2811 5251 Australasia Tel 61 2 9899 0999 Fax 61 2 9899 7511 Austria Tel 01 57606 1619 Fax 01 57606 1627 e Belgium Tel 0800 73 888 Fax 03 272 1637 Canada Tel 800 463 5800 Fax 800 567 1008 Central East amp South East Europe Tel 43 1 982 3826 Fax 43 1 985 8327 Denmark Tel 45 16 2400 Fax 45
22. rformed in a single time saving run Using the Manifold the strips are rehydrated in the reswelling tray and no rehydration time is required Rehydration time can be set from 1 minute to 99 hrs 59 minutes Temperature during rehydration Temperature during rehydration can be set between 15 C and 30 C This value can be set in 1 C increments _ Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 17 Software overview 18 Current The maximal recommended current is 75 yA strip Temperature during the isoelectric focusing run Temperature during isoelectric focusing can be set between 15 C and 30 C This value can be set in 1 C increments Since strips are run in high concentrations of urea low temperatures can lead to the formation of urea crystals that block proper focusing Step Grad The two options for the voltage change pattern are Step which sets the voltage at the selected value for the new step and then holds the voltage constant for the step duration and Grad gradient which increases the voltage linearly with respect to time from the value set in the previous step to the value set for the current step If the first step is set in gradient mode the voltage will increase starting from 0 V Voltage The Voltage of any step may be set from 1 10000 V A value of 0 in this field will cause the program to end regardless of the values set for any subsequent steps
23. rotocol A Save as dialog is opened Change protocol file name and Protocol directory folder if desired Run settings amp details Session log Protocol file Number of strips GIB aoi Note Ifthe name ofan edited protocol is not changed the protocol used for editing will be overwritten and lost 5 2 5 Downloading protocols to instrument at specific positions 1 To download a protocol to the Ettan IPGphor 3 instrument at another position than position 1 select menu Protocol Transfer protocol and select desired Instrument position 1 10 Click OK Transfer protocol to instrument x Instrument position 6 z 2 The current protocol is now downloaded to the selected position in the Ettan IPGphor 3 instrument Any existing protocol at that position is overwritten 5 2 6 Deleting protocols 1 Click on the right button of the three buttons in the top of the Protocol view The current protocol is now deleted Run settings amp details Session log Protocol file Number of strips e arl Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 29 Ee Setting protocols 53 Printing protocols 1 Open the menu File Print Ctrl P 2 Click the Print option Current protocol tick box and OK Print xi Print options Display screen IV Current protocol I Session log 30 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Startin
24. rs Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 7 KD Introduction Se 1 2 Associated documentation e Ettan IPGphor 3 Instrument User Manual 11 0034 58 e Ettan IPGphor Cup Loading Manifold User Manual 11 0034 60 e Ettan IPGphor 3 Safety Handbook 11 0034 61 e Instructions Immobiline Dry Strip attached to each package of Immobiline Dry Strip 8 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Requirements and installation 3 2 Requirements and installation 2 1 Computer requirements Computer Ettan IPGphor 3 Control Software is designed to work with computers that have operative systems Microsoft Windows XP or Microsoft Windows 2000 The computer needs to have a COM port or a USB port To print displays protocols session logs etc a printer has to be connected to the computer To export and save run data graphs and log files Microsoft Excel software is required Excel 2002 or later Computer instrument connection Use the serial cable that comes with Ettan IPGphor 3 isoelectric focusing Unit to connect the serial port located at the back of the Ettan IPGphor 3 instrument to the appropriate COM port of the computer USB port can also be used together with a USB to RS232 converter see Ordering information in Ettan IPGphor 3 Instrument User Manual If multiple Ettan IPGphor 3 isoelectric focusing Units are to be controlled by Ettan IPGphor 3 Control Software the user mu
25. st add an RS232 expansion box Such expansion modules are available from National Instruments Select the appropriate expansion module to fit with your computer connections e g USB to RS232 PCMCIA to RS232 Ethernet to RS232 2 2 Software installation 1 Uninstall previous versions of Ettan IPGphor Control Software if present 2 Insert Ettan IPGphor 3 Control Software CD 3 Run Setup exe which is found in the Installation folder on the CD 4 During installation the following directories are created e C IPGphor 3 Protocols e C IPGphor 3 Run Data 5 At the end of the installation a dialog for adding the GE protocols to the registry appears Click Yes in this dialog before restarting the computer Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 9 ED Requirements and installation 10 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 3 Software overview Software overview 3 1 Application window The Ettan IPGphor 3 Control Software application window has a menu bar at the top with header options selections a series of control buttons and two tabs Run settings amp details tab and Session log tab In the Run settings amp details tab there are two views the Info and data view and the Protocol view Tool tips will appear as the mouse is moved across active buttons to guide the user in navigating and using the software Online help for several of these functions is obtained using the menu
26. trol Software or click on Ettan IPGphor 3 Control Software icon on desktop 4 2 Connecting instruments One to four instruments can be connected to one computer and Ettan IPGphor 3 Control Software 1 Open the menu Communication and either of the four Instrument 1 4 options 2 Set communication port below Com port in the dialog box Setting for instrument 1 x Active Com port Name Connected Instrument 1 Iv koma alpha 9 3 Enter a name for the instrument in the Name box This name will be displayed below the instrument buttons in the main software application window when this instrument is selected by the instrument buttons reaf na nes se Tick the Active box to activate the instrument The box now contains a 1 Alpha mark and a green lamp is lit in the corresponding Instrument button Note Sometimes the connection between the Ettan IPGphor 3 Control Software and an instrument is lost If that happens open the menu Communication Instrument 1 4 as described and tick the Active box to activate the instrument When contact to the instrument is successfully established a green light is lit Note Ifthere is frequent instrument computer disconnection ensure that the Ettan IPGphor 3 instrument Baude rate is set to 9600 Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB 23 4 Starting the software and connecting instruments 43 Closing the software Chose File Quit Ctrl Q to close the softwar
27. un e At the top of the tab there are buttons and ComboBoxes used for entering experimental parameters and for protocol editing and handling e At the bottom left of the tab there is a protocol details graph in which setpoint voltage measured voltage and measured current can be displayed e At the bottom right of the tab either Info and Data view or Protocol view is shown 3 4 1 ComboBoxes for entering experimental conditions Fast mode To enable automatic protocol selection in protocol Fast mode Strip length pH range and Number of strips 1 12 have to be set In the ComboBoxes allowed values are shown Run settings amp details Session log Strip length pH range Number of strips of Lo l Fig 3 3 ComboBoxes for experimental parameters in fast mode protocol selection Advanced mode When using the Advanced mode protocol handling is performed with the Protocol file ComboBox and the three buttons see 3 4 2 Number of strips is set by the Number of strips ComboBox Run settings amp details Session log Protocol file Number of strips m BLS B 7 Fig 3 4 ComboBoxes and buttons in advanced mode protocol selection Ettan IPGphor 3 Control Software User Manual 11 0034 59 Edition AB Software overview 3 4 2 Advanced mode protocol handling Use the Protocol file ComboBox and the three buttons to the right of the ComboBox to create a new protocol open an existing protocol o
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