JA-20 rotor user manual
Contents
1. may also be used to isolate and band different classes of subcellular organelles Up to 400 mL of gradient and sample can be centrifuged per run The rotor body and lid are made of aluminum and are anodized for corrosion resistance the rotor is black and the lid is blue O rings made of Buna N rubber in the lid and rotor body maintain atmo spheric pressure inside the rotor during centrifugation if they are properly lubricated Two vertical pins in the rotor drive hole mate with grooves in the centrifuge drive hub in older model centrifuges or with drive hub teeth new model centrifuges to prevent the rotor from slipping during acceleration and deceleration The rotor has been tested to demonstrate containment of microbio logical aerosols under normal conditions of the associated Beckman Coulter centrifuge when used and maintained as instructed The JA 20 rotor 1s warranted for 7 years see the Warranty PREPARATION AND USE Specific information about the JA 20 rotor is given here Use the J Series Rotors and Tubes manual JR 1M along with this rotor manual for complete rotor and accessory operation Validation of microbiological containment was done at an independent third party testing facility CAMR Porton Down UK or USAMRIID Ft Detrick MD U S A Improper use or maintenance may affect seal integrity and thus containment PRERUN SAFETY CHECKS ROTOR PREPARATION JA 20 Rotor I gt NOTE Although rotor com2. Apply appropriate decontamination procedures to the centrifuge and accessories Never exceed the maximum rated speed of the rotor and labware in use Refer to the section on RUN SPEEDS Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use JA 20 Rotor JA 20 FIXED ANGLE ROTOR Pi Axis of Rotation SPECIFICATIONS Maximum speed 000 cece eect eee 20 000 rpm Critical speed range 2 2 eee 600 to 800 rpm Density rating at maximum speed 00 000 ee aee 1 2 g mL Maximum allowable imbalance of opposing loads 2 grams Relative Centrifugal Fieldt at maximum speed Atrmax 108 mm sese eee 48 400 x g At ray 70 mm 1 0 eee eee eee 31 400 x g Atfgmin 22mm i pedis Bhd WERRXWERR ae ee e 14 300 x g k factor at maximum speed eee 769 Conditions requiring speed reductions see RUN SPEEDS Number of tube cavities 00 eee eee eens 8 Available tubes eroe p Sede ceed SESH SPARKS TEARS see Table 3 Nominal tube dimensions largest tube 29 x 104 mm Nominal tube capacity largest tube 00008 50 mL Nominal rotor capacity 2 0 nessau anea 400 mL Approximate acceleration time to maximum speed rotor fully loaded 1 0 0 eee eee 1 2 min Approximate
3. UEM SEES 364914 Hold down knob for use with filtration tubes normally run without lid J2 J6 series centrifuges only 392889 Tubes and bottles ise cerc on Sok lA A deo ue des see Table 3 Spinkote lubricant 202 llle eee 306812 Silicone vacuum grease 102 2 eee eese 335148 Rotor Cleaning Kit llle 339558 Beckman Solution 555 1 qt 2 2 ec eee 339555 Rotor cleaning brush 000s eee cee eee 339379 Rotor removal tool erreser orien eee eens 338896 Collar adapter for rotor removal tool 0 0 00 e ee eee 338689 J SERIES ROTOR WARRANTY Subject to the conditions specified below and the warranty clause of the Beckman Coulter Inc terms and conditions of sale in effect at the time of sale Beckman Coulter Inc agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within seven 7 years after delivery of a J series rotor to the original buyer by Beckman Coulter Inc or by an authorized represen tative provided that investigation and factory inspection by Beckman Coulter Inc discloses that such defect developed under normal and proper use Should a Beckman Coulter cen trifuge be damaged due to a failure of a rotor covered by this warranty Beckman Coulter will supply free of charge all cen trifuge parts required for repair REPLACEMENT Any product claimed to be defective must if requested by Be
4. contact Gammasonics Pty Ltd Australia in Japan contact Toyo Medic Co Ltd Tokyo In U S contact Biodex Medical Systems Shirley New York internationally contact the U S office to find the dealer closest to you 5 Flammability hazard Do not use in or near operating centrifuges 20 STORAGE RETURNING A ROTOR JA 20 Rotor When the rotor is not in use store it in a dry environment not in the instrument with the lid removed to allow air circulation so moisture will not collect in the tube cavities Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc This RGA form may be obtained from your local Beckman Coulter sales office and should contain the following information serial number history of use approximate frequency of use reason for the return original purchase order number billing number and shipping number if possible name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory and name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that all parts are free from pathogens and or radioactivity Steriliza tion and decontamination must be done before
5. rare cases the rotor pins can rest on top of the drive spindle hub pins or teeth and not seat on the hub surface This can prevent the rotor from being secured to the drive spindle Turning the rotor slightly after installing it will move the pins into proper position 4 Place the lid on the rotor and turn the lid knob to the right clockwise until secure Do not overtighten To ensure that the JA 20 rotor reaches the required temperature during the run follow the appropriate instructions below for the model of J centrifuge being used Avanti J Series Centrifuges Enter the run temperature according to the instructions in your centrifuge instruction manual No additional input is required JA 20 Rotor Microprocessor Controlled J2 and J6 Series Centrifuges Models J2 MI J6 MI J2 21M J2 MC J6 MC and J2 21 M E Enter rotor code 20 2 then follow the steps below 1 Press the TEMP key on the centrifuge control panel and then use the keypad to enter the sample temperature Find the compensation value in Table 1 that corresponds with the set temperature and run speed The compensation values listed in Table 1 are approximates for microprocessor controlled centri fuges If precise temperature control is required determine the compensation settings empirically as follows a Fill the rotor with water filled tubes or bottles Precool the rotor and water to the required temperature b Place the filled precooled roto
6. returning the parts Smaller items such as tubes bottles etc should be enclosed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the out side of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label printed on the RGA form when mailing the rotor and or accessories Customers located outside the United States should contact their local Beckman Coulter office 21 JA 20 Rotor 22 SUPPLY LIST I gt NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1 800 742 2345 in the United States or by contacting your local Beckman Coulter office Contact Beckman Coulter Sales 1 800 742 2345 in the United States or your local Beckman Coulter office or see the High Perfor mance High Speed High Capacity Rotors Tubes amp Accessories catalog BR 8102 for detailed information on ordering parts and supplies For your convenience a partial list is given below REPLACEMENT ROTOR PARTS OTHER JA 20 rotor assembly 000s 334831 Lid assembly ie speeches 368998 Lid attachment bolt assembly n nnnnnnnnn na 358305 Osnng Udea eR e E ho E r oR 870612 Osring TOLOP o ester wae Gea e e GER RS 811656 Tie downkit 4 e E ane db gut UH RRCHR
7. to that setting Interpolate if intermediate values are required Table 1 J2 21 and J2 HS Centrifuge Temperature Settings Rotor Required Sample Temperature C Speed rpm 20 10 22 5g 10 20 40 20 000 N N 9 8 7 7 6 18 000 N N 6 6 5 4 3 15 000 5 4 4 4 3 2 1 10 000 2 2 1 0 0 0 0 An N indicates that the rotor cannot achieve the required temperature at this speed Table 2 J2 HC Centrifuge Temperature Settings Rotor Required Sample Temperature C Speed rpm 20 10 22 By 10 20 40 18 000 N N 6 6 5 4 3 15 000 6 5 4 4 3 3 2 10 000 4 4 3 2 2 2 2 An N indicates that the rotor cannot achieve the required temperature at this speed JA 20 Rotor REMOVAL AND SAMPLE RECOVERY N CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories 1 Unscrew the rotor lid to release the rotor from the spindle hub 2 Remove the rotor by lifting it straight up and off the drive spindle If the rotor sticks to the drive spindle hub a collar adapter 338689 and rotor removal tool 338896 may be used a Remove the lid and small O ring from the rotor b Slide the flanges of the collar into the empty O ring groove c Screw the larger end of the rotor remov
8. 55 for use with rotors and accessories Dilute the detergent 10 to 1 with water I gt NOTE Do not wash rotor components in a dishwasher Do not soak in detergent solution for long periods such as overnight 3 Thoroughly rinse the cleaned rotor and components with distilled water 4 Air dry the rotor and lid upside down Do not use acetone to dry the rotor 5 Apply a thin even coat of silicone vacuum grease to the lid O ring before replacing it in the groove in the outer rim of the lid Also apply silicone vacuum grease to the rotor O ring before reassembly Clean metal threads every 6 months or as necessary Use a brush and concentrated Solution 555 Rinse and dry thoroughly then lubricate lightly but evenly with Spinkote to coat all threads Periodically remove the O rings and wipe clean as necessary Clean the O ring grooves with a cotton tipped swab Reapply a light film of silicone vacuum grease If the rotor and or accessories becomes contaminated with radioac tive material it should be decontaminated using a solution that will not damage the anodized surfaces Beckman Coulter has tested a 19 JA 20 Rotor number of solutions and found two that do not harm anodized aluminum RadCon Surface Spray or IsoClean Solution for soaking and Radiacwash IIIB NOTE IsoClean can cause fading of colored anodized surfaces Use it only when necessary and remove it promptly from surfaces While Beckman Coulter ha
9. 900 12 300 5 600 4 000 1 940 1 250 573 12 000 17 400 11 300 5 160 3 500 1 480 960 439 Relative Centrifugal Fields JA 20 Fixed Angle Rotor 50 000 45 000 40 000 p 35 000 lmax x 30 000 Tay 5 25 000 i c l min 20 000 15000 10 000 5 000 2 500 5 000 7 500 10 000 Speed rpm 12 500 15 000 17 500 20 000 JA 20 Rotor CARE AND MAINTENANCE MAINTENANCE Lid 368998 Lid O Ring Lo 870612 7 Rotor O Ring Check 811656 for Corrosion Rotor Body 18 I gt NOTE Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use Periodically at least monthly inspect the rotor especially inside cavities for rough spots or pitting white powder deposits frequently aluminum oxide or heavy discoloration If any of these signs are evident do not run the rotor Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center Before using the rotor inspect the rotor drive pins to ensure that they are not damaged Damaged drive pins can prevent the rotor from seating properly on the centrifuge drive hub Avanti J series centrifuges only To inspect the drive pins turn the rotor upside down and look into the drive hole in the center of the ro
10. For example at 20 000 rpm mammalian viruses s 700 S can be pelleted in t 1 1 hours 4 Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows 2 20 000 ka E oe adj ctual run speed 15 JA 20 Rotor 16 RUN SPEEDS SPEED RPM RCF Run times can also be estimated from data established in prior experi ments using a different rotor if the k factor of the previous rotor is known For any two rotors a and b 2 6 For more information on k factors see Use of k Factor for Estimating Run Times from Previously Established Run Conditions publication DS 719 The centrifugal force at a given radius in a rotor is a function of speed Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields RCF When rota tional speed is adjusted so that identical samples are subjected to the same rcf in two different rotors the samples are subjected to the same force The RCF at a number of rotor speeds is provided in Table 4 Do not select rotational speeds higher than the maximums you have determined to be appropriate for your protocols in no case above 20 000 rpm In addition when centrifuging solutions more dense than 1 2 g mL in this rotor calculate the reduced maximum allowable rotor speed reduced maximum speed 20 000 rpm 12 g mL 7 p where p is the density of the tube contents Note
11. J TB 003AZ Em November 2008 COULTER JA 20 Fixed Angle Rotor Used In Beckman Coulter J2 J6 and Avanti J Series Centrifuges 2008 2009 Beckman Coulter Inc JA 20 Rotor SAFETY NOTICE This safety notice summarizes information basic to the safe use of the rotor described in this manual The international symbol displayed above is a reminder to the user that all safety instructions should be read and understood before operation or maintenance of this equipment is attempted When you see the symbol on other pages throughout this publication pay special attention to the specific safety informa tion presented Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the rotor This rotor was developed manufactured and tested for safety and reliability as part of a Beckman Coulter centrifuge rotor system Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter s approval N pepe qeu Ip d Handle body fluids with care because they can transmit disease No known test offers complete assurance that such fluids are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other i
12. OPERATION INSTALLING THE ROTOR Figure 1 Arranging Tubes in the Rotor Two four six or eight tubes can be centrifuged per run if they are arranged in the rotor as shown For low temperature runs precool the rotor in the centrifuge or in a refrigerator before use especially before short runs to ensure that the rotor reaches the set temperature A suggested precooling cycle is a minimum of 30 minutes at 2000 rpm at the required temperature If fluid containment is required use capped tubes or bottles It is strongly recommended that all containers carrying physiological fluids be capped to prevent leakage Ifyou are using an Avanti J series centrifuge select the JA 20 rotor If you are using an Avanti J E centrifuge enter rotor code 20 If you are using a microprocessor controlled J2 or J6 series centrifuge enter rotor code 20 2 Refer to your centrifuge instruction manual for additional information Two metal pins inside the rotor drive hole engage with teeth on the centrifuge drive hub to prevent the rotor from slipping during acceler ation and deceleration see Figure 2 When the rotor is properly installed the pins sit either in the drive hub grooves or on top of the hub surface see Figure 3 In rotors manufactured since early 1997 the rotor pins are positioned parallel to the BECKMAN name engraved at the center of the rotor body see Figure 4 Knowing the pin orientation before you install the rotor will h
13. al tool into the threaded opening of the collar adapter As the removal tool tightens down it will push against the drive spindle hub causing the rotor to rise d Remove the tool and collar adapter from the rotor by reversing the above procedure e Replace the O ring in the groove f Remove the rotor from the centrifuge III NOTE Lubricate J2 and J6 series centrifuge drive hubs with Spinkote to prevent the rotor from sticking Avanti J series centrifuges have Delrin rings on the hubs to prevent sticking and do not require lubrication Delrin is a registered trademark of E I Du Pont de Nemours amp Company 11 JA 20 Rotor 12 TUBES AND BOTTLES N a o Oo Qmm 2j WS M C The JA 20 rotor uses the tubes and bottles listed in Table 3 Be sure to use only those items listed and to observe the maximum speed limits and fill volumes shown Maximum fill volume is the maximum amount that can be centrifuged in the container listed Refer to Appendix A in Rotors and Tubes for chemical compatibilities of tube bottle and accessory materials Temperature Limits Plastic containers have been centrifuge tested for use at temp eratures between 2 and 25 C For centrifugation at other temperatures pretest tubes under anticipated run conditions fplastic containers are frozen before use make sure that they are thawed to at least 2 C prior to centrifugation Thickwall Tubes Thic
14. ckman Coulter Inc be returned to the factory transporta tion charges prepaid and will be returned to Buyer with the transportation charges collect unless the product is found to be defective in which case Beckman Coulter Inc will pay all transportation charges A defective rotor will be replaced by Beckman Coulter Inc at its then current list price less a credit based upon the age of the rotor years since date of purchase The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter s Indianapolis Indiana facility or delivered to a Beckman Field Service representative The replacement price cost to Buyer for the respective rotor shall be calculated as follows years Replacement price Current rotor list price x CONDITIONS 1 Except as otherwise specifically provided herein this war ranty covers the rotor only and Beckman Coulter Inc shall not be liable for damage to accessories or ancillary supplies including but not limited to i tubes ii tube caps iii tube adapters or iv tube contents 2 This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance con trary to the instructions in the Beckman Coulter rotor or centrifuge manual 3 This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor charac teristics or is operated in a Beckman Coulter centrifuge that
15. deceleration time from maximum speed rotor fully loaded nanunua 2 1 2 min Weight of fully loaded rotor nunnana 7 4 kg 16 5 Ib Rotor material 2s cR RUE ET ea XR em aluminum Rotor entry code for J2 and J6 series microprocessor controlled centrifuges llle esee 20 2 Rotor entry code for Avanti J E centrifuges 0000 20 Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed ro to the standard acceleration of gravity g according to the following formula RCF 79 amp where r is the radius in millimeters is the angular velocity in radians per second 2 z RPM 60 and g is the standard acceleration of gravity 9807 mm s After substitution RP 2 RCF 1 12r E JA 20 Rotor DESCRIPTION Lid 368998 Lid O Ring _ _ 870612 Rotor O Ring 811656 V Rotor Body P This rotor has been manufactured in a registered ISO 9001 or 13485 facility for use with the specified Beckman Coulter centrifuges The JA 20 rated for 20 000 rpm is a fixed angle rotor designed to centrifuge up to eight 50 mL tubes at a 34 degree angle to the axis of rotation Used in Beckman Coulter J2 J6 and Avanti J series centri fuges the rotor develops centrifugal forces that can efficiently pellet bacteria cell membranes and subcellular particles Short column methods such as partially filled tubes
16. e guidelines JA 20 Rotor Table 3 Available Tubes and Bottles for the JA 20 Fixed Angle Rotor continued Tube Max Required Accessory No Max Fill Tubes Speed 4 Dimensions Part Vol Part per RCF and Volume Description Number mL Description Number Adapter k Factor 16 x 80 mm polycarbonate 355672 10 adapter 342327 1 20 000 rpm 10 mL bottle screw cap polyethylene 43 500 x g 870329 650 rubber 11 x 39 mm Microfuge tube 340196 1 8 adapter 344497f 3 20 000 rpm 1 8 mL natural polyethylene 48 400 x g 769 11x 39 mm Microfuge tube 357448 1 5 adapter 344497f 3 20 000 rpm 1 5 mL natural polyallomer 48 400 xg snap on cap 769 11x 39 mm Microfuge tube 356090 1 5 adapter 344497f 3 20 000 rpm 1 5 mL natural 48 400 x g polypropylene 769 snap on cap 11 x 39 mm Microfuge tube 356091 1 5 adapter 344497f 3 20 000 rpm 1 5 mL blue polypropylene 48 400 xg snap on cap 769 11x 39 mm Microfuge tube 356092 1 5 adapter 344497f 3 20 000 rpm 1 5 mL green 48 400 x g polypropylene 769 snap on cap 11x 39 mm Microfuge tube 356093 1 5 adapter 344497f 3 20 000 rpm 1 5 mL yellow 48 400 xg polypropylene 769 snap on cap 11 x 39 mm Microfuge tube 356094 1 5 adapter 344497f 3 20 000 rpm 1 5 mL orange 48 400 x g polypropylene 769 snap on cap a Maximum speeds listed are guidelines only These speeds have been achieved in reliability tests at Beckman Coulter but because of ma
17. elp to ensure that you position the rotor properly on the hub minimizing the chance of hub damage Follow the steps below JA 20 Rotor ECKMAN Rotor Drive Hole Drive Pins Angled pins shown Pins can also be vertical or horizontal Avanti J Centrifuge Drive Spindle Hub Figure 2 Rotor Drive Pin Location and Orientation 1 Turn the rotor until the BECKMAN name is either horizontal or perpendicular to the drive hub pins or teeth see Figure 4 Alter nately rotate the drive shaft to align the drive shaft hub pins or teeth with the rotor drive pins 2 Carefully lower the rotor straight down onto the centrifuge drive spindle hub Drive Rotor Hub Pins 2 Teeth 2 Grooves 4 Drive Hub Drive Hub Drive pos Drive Spindle Spindle Assembly Assembly Older Model Centrifuges Newer Model Centrifuges Be sure the pins in the rotor drive hole Be sure the pins in the rotor drive hole are seated in the grooves are not sitting on top of the teeth of the drive spindle hub as shown on the drive spindle hub Figure 3 Centrifuge Drive Hub Configurations JA 20 Rotor Top View TEMPERATURE TEMP C H Side View nmn mM _ _ e p Figure 4 Installing the Rotor 3 Slowly turn the rotor making sure that it is properly seated on the drive spindle hub In
18. has been improperly disassembled repaired or modified DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT ABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CON SEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT B BECKMAN COULTER Beckman Coulter Inc 250 S Kraemer Blvd Brea California 92821 Sales and Service 1 800 742 2345 Internet www beckmancoulter com 2009 Beckman Coulter Inc All rights reserved
19. however that the use of this formula may still produce maximum speed figures higher than the limitations imposed by the use of certain tubes or adapters see Table 3 In such cases use the lower of the two figures Table 4 Relative Centrifugal Fields for the JA 20 Rotor Entries in this table are calculated from the formula RCF 1 12 r RPM 1000 and then rounded to three significant digits JA 20 Rotor Relative Centrifugal Field x g Relative Centrifugal Field x g Rotor Rotor Speed At Imax At ray At min Speed At Imax At fay At min rpm 108 mm 70 mm 32 mm rpm 108 mm 70 mm 32 mm 20 000 48 400 31 400 14 300 11 500 16 000 10 400 4 740 19 500 46 000 29 800 13 600 11 000 14 600 9 490 4 340 19 000 43 700 28 300 12 900 10 500 13 300 8 640 3 950 18 500 41 400 26 800 12 400 10 000 12 100 7 840 3 580 18 000 39 200 25 400 11 600 9 500 10 900 7 080 3 240 17 500 37 000 24 000 11 000 9 000 9 800 6 350 2 900 17 000 35 000 22 700 10 400 8 500 8 740 5 660 2 590 16 500 32 900 21 300 9 760 8 000 7 740 5 020 2 290 16 000 31 000 20 100 9 180 7 500 6 800 4410 2 020 15 500 29 100 18 800 8 610 7 000 5910 3 840 1760 15 000 27 200 17 600 8 070 6 500 5 110 3310 1510 14 500 25 400 16 500 7 540 6 000 4 360 2 820 1 290 14 000 23 700 15 400 7 030 5 500 3 660 2 370 1 080 13 500 22 100 14 300 6 530 5 000 3 020 1 960 896 13 000 20 400 13 300 6 060 4 500 2450 1 590 726 12 500 18
20. kwall polyallomer polypropylene and polycarbonate tubes can be run partially filled at least half filled with or without caps but all opposing tubes for a run must be filled to the same level with liquid of the same density Do not overfill capless tubes be sure to note the fill volumes and run speeds shown in Table 3 Polycarbonate and Polyallomer Bottles Capped polycarbonate and polyallomer bottles may be centrifuged completely filled or partially filled not less than half full Again all opposing containers for a run must be filled to the same level Be sure to note the reductions in run speed shown in Table 3 if bottles are partially filled Microfuge Tubes Microfuge tubes are placed in multi tube adapters for use in this rotor The number and arrangement of tubes in opposing adapters should be balanced Table 3 Available Tubes and Bottles for the JA 20 Fixed Angle Rotor Use only the items listed here and observe fill volumes and maximum run speeds JA 20 Rotor Tube Max Required Accessory No Max Fill Tubes Speed 4 Dimensions Part Vol Part per RCF and Volume Description Number mL Description Number Adapter k Factor 29 x 104 mm polycarbonate 3570006 45 none 20 000 rpm 50 mL bottle assy liquid 48 400 xg tight cap assy 769 29 x 104 mm polyallomer bottle 357001 45 none 20 000 rpm 50 mL assy liquid tight 48 400 x g cap ass
21. nfectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol contain ment Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials in nor handle or store them near the centrifuge Although rotor components and accessories made by other manufacturers may fit in the JA 20 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the JA 20 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Make sure that filled containers are loaded symmetrically into the rotor and that opposing tubes are filled to the same level with liquid of the same density If disassembly reveals evidence of leakage and pathogenic or radioactive materials are involved you should assume that some fluid escaped the rotor
22. nufacturing variances no guarantee of performance or fit is expressed or implied Bottles 357000 and 357001 use cap 356284 cap assembly insert 358627 order only as a replacement part and O ring 961582 Polypropylene package of 25 4 Above 20 C fill polypropylene tubes at least half full Commercially available Observe manufacturer s speed and fill volume guidelines f Delrin Can be stacked force will vary depending on tube position Delrin is a registered trademark of E I Du Pont de Nemours amp Company 14 RUN TIMES TIME HR MIN Fia i Li Ll Ll JA 20 Rotor The k factor of the rotor is a measure of the rotor s pelleting efficiency Beckman Coulter has calculated the k factors for all of Its rotors at maximum speed with full tubes using the following formula E In max min is 10 1 p 3600 where cis the angular velocity of the rotor in radians per second c 0 105 x rpm rmax is the maximum radius and rq is the minimum radius After substitution 2 533 x 10 In max Iris k 2 rpm In the JA 20 rotor the k factor is 769 for full 50 mL tubes For containers that nominally hold less than 50 mL the amp factors will be different Calculate k factors for these containers using equation 2 Use the k factor in the following equation to estimate the run time t in hours required to pellet particles of known sedimentation coeffi cient s in Svedberg units S 3
23. ponents and accessories made by other manufacturers may fit in the JA 20 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in this rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Read the Safety Notice page at the front of this manual before using the rotor 1 Make sure that the rotor and lid are clean and show no signs of corrosion or cracking 2 Check the chemical compatibilities of all materials used Refer to Chemical Resistances in Appendix A of the J Rotors and Tubes Manual 3 Verify that the tubes and bottles being used are listed in Table 3 For runs at other than room temperature refrigerate or warm the rotor beforehand for fast temperature equilibration l Be sure that metal threads in the rotor are clean and lightly but evenly lubricated with Spinkote lubricant 306812 Also ensure that O rings are lightly but evenly coated with silicone vacuum grease 335148 Load the filled containers symmetrically into the rotor Refer to TUBES AND BOTTLES on page 12 for information about containers If fewer than eight tubes are being run they must be arranged symmetrically in the rotor see Figure 1 Opposing tubes must be filled to the same level with liquid of the same density JA 20 Rotor
24. r in the centrifuge and run the system for at least 2 hour c Measure the water temperature If it is lower than the required run temperature enter positive compensation units during run setup following steps 3 and 4 below If it is higher than the required run temperature enter negative compensation units during run setup also using steps 3 and 4 below d Discard the water Fill tubes with sample load tubes into the rotor and precool the system to the required temperature before starting the run Press COMP ADJ The word COMP flashes below the TEMPERATURE display and the display flashes Use the keypad to enter the required compensation value Press the key to enter a minus sign pressing it again will remove the minus sign Check the temperature display If the entry is incorrect press CE and reenter the digits When the entry is correct press ENTER RECALL I gt NOTE To clear a COMP ADJ entry press COMP ADJ 0 and ENTER RECALL JA 20 Rotor 10 SET O COMP Analog J2 Series Centrifuges Enter the required run temperature and the appropriate temperature compensation units see Table 1 or 2 on the centrifuge control panel as follows 1 Turn the SET knob to the required sample temperature 2 Find the compensation value Table 1 for Models J2 HS and J2 21 Table 2 for Model J2 HC that corresponds to the required temperature and run speed Set the COMP dial
25. s tested these methods and found that they do not damage components no guarantee of decontamination is expressed or implied Consult your laboratory safety officer regarding the proper decontamination methods to use Ifthe rotor or other components are contaminated with toxic or patho genic materials follow appropriate decontamination procedures as outlined by your laboratory safety officer STERILIZATION AND DISINFECTION 121 C ED Guo The rotor and all rotor components can be autoclaved at 121 C for up to an hour Remove the lid from the rotor and place the rotor and lid in the autoclave upside down Ethanol 70 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discoloration of anodized surfaces Use the minimum immersion time for each solution per laboratory standards While Beckman Coulter has tested these methods and found that they do not damage the rotor or components no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfec tion is a concern consult your laboratory safety officer regarding proper methods to use Refer to publication IN 192 included in each box of tubes or bottles for tube and bottle sterilization and disinfection procedures 3 In U S contact Nuclear Associates New York in Eastern Europe and Commonwealth States contact Victoreen GmbH Munich in South Pacific
26. tor If the drive pins appear damaged contact Beckman Coulter Field Service Routinely apply Spinkote lubricant 306812 to the centrifuge drive spindle hub to prevent the rotor from sticking not required on Avanti J centrifuges Regularly lubricate the metal threads in the rotor with a thin even coat of Spinkote lubricant Failure to keep these threads lubricated can result in damaged threads Regularly apply silicone vacuum grease to the O rings Replace O rings about twice a year or whenever worn or damaged Refer to Appendix A in Rotors and Tubes for chemical compatibili ties of rotor and accessory materials Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center CLEANING Em ry a Rotor Cleaning Kit 339558 Lid O Ring ert 870612 Rotor O Ring 811656 DECONTAMINATION JA 20 Rotor Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred Do not allow corrosive materials to dry on the rotor Under normal use wash the rotor frequently at least weekly to prevent buildup of residues 1 Remove the O rings before washing 2 Wash the rotor and lid in a mild detergent such as Beckman Solution 555 that won t damage the rotor The Rotor Cleaning Kit 339558 contains two special plastic coated brushes and two quarts of Solution 5
27. y 769 29 x 104 mm polycarbonate 357002 40 none 20 000 rpm 50 mL bottle screw cap 48 400 xg 769 29 x 104 mm polyallomer bottle 357003 40 none 20 000 rpm 50 mL Screw cap 48 400 xg 769 29 x 104 mm polycarbonate 363647 36 5 white 29 mm cap 356624 20 000 rpm 50 mL thickwall tube red 29 mm cap 357359 48 400 xg snap on cap green 29 mm cap 357360 769 yellow 29 mm cap 357361 blue 29 mm cap 357362 29 x 104 mm polypropylene 357005 36 54 white 29 mm cap 356624 20 000 rpm 50 mL thickwall tube red 29 mm cap 357359c 48 400 xg snap on cap green 29 mm cap 357360 769 blue 29 mm cap 357361 357362 29 x 104 mm polypropylene 357007 354 none 20 000 rpm 50 mL thickwall tube 48 400 xg no cap 769 29 x 105 mm glass tube e e adapter 870331 1 e 30 mL rubber 17 x 100 mm glass tube e e adapter 870329 1 e 15 mL rubber 16 x 100 mm glass tubee e e adapter 870329 1 e 15 mL rubber continued a Maximum speeds listed are guidelines only These speeds have been achieved in reliability tests at Beckman Coulter but because of manufacturing variances no guarantee of performance or fit is expressed or implied P Bottles 357000 and 357001 use cap 356284 cap assembly insert 358627 order only as a replacement part and O ring 961582 Polypropylene package of 25 4 Above 20 C fill polypropylene tubes at least half full Commercially available Observe manufacturer s speed and fill volum
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