HVC-03M - NPI Electronic Instruments
Contents
1. or for showing the electrode potential in mV VceL at display 2 version 2 5 page 9 HVC 03M User Manual 4 Cell Model optional 4 4 Introduction The cell model is designed to be used to check the function of the instrument either 1 just after unpacking to see whether the instrument has been damaged during transport or to train personnel in using the instrument or 3 incase of trouble to check which part of the setup does not work correctly e g to find out whether the amplifier is broken or if something is wrong with the electrodes or holders etc This cell model consist only of passive elements 1 e resistors that simulate the resistance of the cell membrane and the electrodes and a capacitance that simulates the capacitance of the cell membrane see Figure 5 A switch allows to simulate two different cell conditions one with 10 MQ membrane resistance and a second with 1 MQ membrane resistance The membrane capacitance is always 5 nF Note This cell model is not suitable for SEC 03M amplifiers in single electrode operation 4 5 Cell Model Description r BNC jack HV 4aV GND on 1 MO CELL membrane R switch i a 10 MiO Ce amp NF R 33 MQ SEC SUBCLICK connector Figure 5 HVC passive cell model version 2 5 page 10 HVC 03M User Manual HV 45V BNC connector for the headstage of the HVC 03M current resistance 33 MQ GND ON switch switch to ground the current headstage for BIAS2. And OPETAN enei a AEA AEE 11 Checking the Configuration with the Cell Model ssnnnnnssssssssoeeeessssssseerssssssssseeeees 11 Se Testand Tumne Procedures resna a a 12 5 1 Current Headstage Bias Current Adjustment cc eccccccccccceeeseseeeceeeeeeeaeeeeseeeeeees 12 5 2 Capacitance Compensa Onea a cael daca eae taeelewtGat tecatonwt a a Gaede 13 Oe 0651 0 018 C0 eepreereem ners Uren E nee Oe eet at een tt em eee nn Oyo eter eerie Tt 15 Ol C Onne COINS sori ats ce es ache hea ire a canes cate cee sacar cach ove eae 15 Ta EC MAC Al EY AU n E O E 16 version 2 5 page 2 HVC 03M User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other life supporting system npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 GENERAL This system is designed for use in scientific laboratories and must be operated only by trained staff General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriat
3. accuracy of the system The HVC 03M is equipped with a high voltage current source that is connected to the current injecting electrode and performs the current injection This current source has a high impedance floating output Therefore the zero point the zero of the bias current of the current source must be defined i e without an input signal there should not be an output current Since the high voltage FET amplifiers that are used become warm from the internal heat dissipation and their characteristics are strongly temperature dependent the calibration procedure has to be done periodically by the user The tuning procedure is done using the BIAS control and one resistance of a few Q and one of a few MQ It is based on Ohm s Law U R D If the headstage generates an output current this current will cause a voltage deflection at a test resistor This voltage deflection is proportional to the output current and is displayed on the digital meter Using the BIAS control the monitored voltage can be set to 0 The tuning procedure is performed using high value resistors or the cell model It cannot be performed with an electrode since there are always unknown potentials involved tip potential junction potentials etc Warning High voltage Always turn power off when working directly on the current headstage output see Chapter 1 Ld Switch the control selector switch 3 to off This ensures that no external signal affects th
4. an electrode resistance measurement unit and correction for BIAS current Important A EPMS 07 H housing with a high voltage power supply is required for the HVC 03M as indicated at the rear panel of the EPMS 07 H housing The HVC 03M can also be operated as a module for iontophoresis using an external control signal see below version 2 5 page 6 HVC 03M User Manual 3 3 Description of the Front Panel and Operation HVC 03M noi O OFF Rs COMP CUR OFFSET Figure 4 HVC 03M front panel view In the following description of the front panel elements each element has a number that is related to that in Figure 4 The number is followed by the name in uppercase letters written on the front panel and the type of the element in lowercase letters Then a short description of the element is given 1 DUAL LED amy LED indicating the status of the HVC 03M Lights if the connected SEC 03M is active The DUAL LED of the respective SEC 03M amplifier lights as well The LED lights also if the control selector switch 5 is in EXT position e g for iontophoresis 2 Potential resistance display Display showing the potential at the electrode tip in mV the electrode resistance in MQ or the current flowing in nA The value shown here is also provided at OUT connector 9 version 2 5 page 7 HVC 03M User Manual 3 BIAS trim pot Trim pot for adjusting the BIAS current BIAS 4 CAP COMP potentiometer Po
5. compensation range 200 mV EXT IN 100 nA 1V Size front panel 12 HP 60 6 mm x 3U 128 5 mm 7 175 mm deep EPMS 07 SYSTEM Power requirements 115 230 V AC 60 50 Hz fuse 2 A 1 A slow 45 60 W dependent on the modules plugged in Dimensions 19 rackmount cabinet 3U high 1U 1 4 44 45 mm version 2 5 page 16
6. Electronic Instruments for the Life Sciences pAs e ade OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE HVC 03M HIGH VOLTAGE CLAMP MODULE FOR EPMS SYSTEMS HVC 03M npi DUAL VERSION 2 5 npi 2014 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com HVC 03M User Manual Table of Contents l cially JING OULALI OMe sake late Marlene hee maker desta honed a ah hen anh 3 2 JEPINS 07 Modular Plun Syste Maene ee a E 4 2 1 General System Description Operation oseere a a 4 22e E PMS 07 TOUSE ssania A 4 29s EPMS EOF HOUS naa a a a a a a ieee 4 DAS PWR OSD eree a a 4 29s SyS GrOUNCIN G eei i a a a a 5 EPM S OTi E A A T A 5 ERMS T Oea r a Nm A a err 5 20e MECHMIC al Dalis N ates eaeiweorsasu aides 5 E et fe A E E E E arse ne N E N E E E E A A E EAT 5 PENS ve O eE E R E ie eer noe 5 3 HVC 03M High Voltage Clamp Modulle cececcccccccssssssecceeccecaeeseeeeeeceeeeeseenseseseeees 6 Sale HYCO M COmpOne is ves cccsciateciet N E bees uninciattasedcansiels 6 op OVA DBL eX i m0 8 1 Ree a E re Te ear ne 6 3 3 Description of the Front Panel and Operation c cc ceeeccccccccccceeeeeeeceeeeeeessaesseeeeees 7 A Wel NMOGE ROPNE tiencei aaa etiahatestiaess 10 Aes MAE OCUIC UL OM passat ined or oer E T A oueamaceeat 10 A Cell Moder Descrip ON sareen E E tamed diaee 10 40s Connections
7. current adjustment see below CELL membrane switch switch for cell membrane representing a membrane of either 10 MQ or 1 MQ resistance SEC SUBD connector for the SEC potential headstage resistance 33 MQ 4 6 Connections and Operation Checking the Configuration with the Cell Model a Turn POWER switch of the EPMS 07 housing off Connect the BNC jack HV 45V of the cell model to the BNC connector of the HVC 03M headstage Connect the SEC 03M headstage to the SMB connector SEC at the cell model Switch the CELL membrane switch see Figure 5 to the desired position Turn all controls at the amplifier to low values less than 1 and the OFFSET in the range of 5 Turn POWER switch of the EPMS 07 housing on Now you can adjust the amplifier and apply test pulses to the cell model Connection to the BNC jack and the SMB connector gives access to the cell via electrodes each with 33 MQ resistance The upper position the CELL membrane switch simulates cell with a resistance of 1 MQ The lower position represents a cell membrane with 10 MQ version 2 5 page 11 HVC 03M User Manual 5 Test and Tuning Procedures 5 1 Current Headstage Bias Current Adjustment Caution It is important that this tuning procedure is performed ONLY after a warm up period of at least 30 minutes The tuning procedure must be performed regularly at least once a month with great care since the bias current changes over time and it determines the
8. e HVC 03M Connect the ELECTRODE connector of the HVC 03M headstage to ground Set switch 11 to VceL Set the reading of the display 2 to 0 using the OFFSET potentiometer 11 O C O vu After tuning the electrode potential OFFSET to zero simulate an electrode by grounding the headstage via 10 MQ resistor L The digital display and the OUT connector 9 now shows a voltage deflection that is related to the BIAS current of the headstage according to Ohm s Law Cancel this voltage by tuning the headstage BIAS trim pot 3 The current is 0 if the voltage deflection is 0 version 2 5 page 12 HVC 03M User Manual 5 2 Capacitance Compensation High resistances of electrodes and stray capacitances form a low pass filter which deteriorates the shape of intracellular stimuli The frequency response of the amplifier is improved considerably by using the capacitance compensation function This function is based on positive feedback negative capacitance circuit The tuning of the capacitance compensation control can be performed using pulses provided by the electrode resistance test circuit or external pulses With the electrode in the bath the CAP COMP control is turned clockwise until there 1s no capacitive artifact on the potential output see Figure 6 1 Immerse the electrode into the bath as deep as necessary during the experiment JI Set switch 11 to VceL and tune the OFFSET to zero I Push the Rce switch 11 Fi
9. e safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore be handled with care Electrostatic discharge can be avoided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition 1 e after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient HANDLING Please protect the device from
10. gure 4 or apply square pulses to the EXT IN BNC 7 Figure 4 switch the control selector switch 5 Figure 4 to EXT and watch the potential output L Compensate the input capacitance as shown in Figure 6 using the CAP COMP potentiometer 4 Figure 4 Figure 6 illustrates the capacitance compensation procedure using a 100 MQ resistor that represents the electrode The upper diagram shows an undercompensated capacitance In the diagram in the middle the capacitance is slightly overcompensated and in the lower diagram it is well compensated version 2 5 page 13 HVC 03M User Manual undercompensated potential mV 200 200 150 100 50 4 0 10 20 30 40 50 time ms overcompensated potential mV 600 400 200 o 200 400 4 600 0 10 20 30 40 50 time ms compensated potential mV 200 7 200 150 100 50 0 j oe j k k EE e i j 0 10 20 30 40 50 time ms potential Figure 6 Tuning of the capacitance compensation using a 100 MQ resistor version 2 5 page 14 HVC 03M User Manual 6 Operation 6 1 Connections The HVC 03M can be used together with an SEC 03M as current module for two electrode voltage current clamp In two electrode voltage clamp mode the SEC 03M does not work as switched amplifier but as continuous mode amplifier for potential registration Connections to the SEC 03M are not required All
11. mmend that you try both arrangements to determine the best configuration a 7 amp EPMS E 07 amp The 19 cabinet is connected to the PROTECTIVE EARTH connector at the rear panel The chassis is linked to protective earth only if the PWR 03D is connected It can be connected also to the SYSTEM GROUND SIGNAL GROUND on the rear panel of the instrument see Figure 3 Important Always adhere to the appropriate safety measures Figure 3 Rear panel connectors of the EPMS E 07 2 6 Technical Data 19 rackmount cabinet for up to 7 plug in units Dimensions 3U high 1U 1 3 4 44 45 mm 254 mm deep EPMS 07 Power supply 115 230 V AC 60 50 Hz fuse 2 A 1 A slow 45 60 W EPMS E 07 External power supply for EPMS E 115 230 V AC 60 50 Hz fuse 1 6 0 8 A slow Dimensions of External power supply W x D x H 225 mm x 210 mm x 85 mm version 2 5 page 5 HVC 03M User Manual 3 HVC 03M High Voltage Clamp Module 3 1 HVC 03M Components The following items are shipped with the HVC 03M system V High voltage clamp module for the EPMS 07 system V User manual V Headstage 3 2 System Description The HVC 03M high voltage clamp module is designed for current injection into cells It extends one or two SEC 03M modules so that two electrode voltage clamp experiments using high voltages can be performed It features OFFSET cancellation capacity compensation of the electrode capacity series resistance compensation
12. moisture heat radiation and corrosive chemicals version 2 5 page 3 HVC 03M User Manual 2 EPMS 07 Modular Plug In System 2 1 General System Description Operation The npi EPMS 07 is a modular system for processing of bioelectrical signals in electrophysiology The system is housed in a 19 rackmount cabinet 3U has room for up to 7 plug in units The plug in units are connected to power by a bus at the rear panel The plug in units must be kept in position by four screws M 2 5 x 10 The screws are important not only for mechanical stability but also for proper electrical connection to the system housing Free area must be protected with covers 2 2 EPMS 07 Housing The following items are shipped with the EPMS 07 housing V EPMS 07 cabinet with built in power supply J Mains cord J Fuse2A 1A slow Front covers In order to avoid induction of electromagnetic noise the power supply unit the power switch and the fuse are located at the rear of the housing 2 3 EPMS E 07 Housing The following items are shipped with the EPMS E 07 housing EPMS E 07 cabinet External Power supply PWR 03D Power cord PWR 03D to EPMS E 07 Mains chord Fuse 1 6 A 0 8 A slow Front covers SSSSSS The EPMS E 07 housing is designed for low noise operation especially for extracellular and multi channel amplifiers with plugged in filters It operates with an external power supply to minimize distortions of the signals caused by the po
13. signals are wired via the EPMS bus For operation as a high voltage clamp module for two electrode voltage current clamp simply connect the headstage to the headstage connector 9 Figure 4 and the current output 6 Figure 4 to an oscilloscope or data acquisition system respectively For potential registration the POTENTIAL OUTPUT x10 mV BNC connector of the SEC 03M is used In two electrode voltage current clamp mode the CURRENT OUTPUT 10 nA V BNC connector of the SEC 03M is disabled and the current output of the HVC 03M 1s used Important Since the HVC 03M has a ten times higher current amplification the CURRENT STIMULUS INPUT of the SEC 03M is now 10 nA V and the holding current range is up to 100 nA The current display at the SEC 03M is not valid The HVC 03M can also be used as amplifier for iontophoresis For operation as a iontophoretic amplifier connect a stimulus generator or a data acquisition system respectively to the EXT IN BNC connector 8 Figure 4 und set the control selector switch 3 Figure 4 to EXT The HVC 03M generates a current proportional to the voltage at EXT IN BNC scaling 100 nA 1 V Note All signal outputs are coarse and unfiltered directly from the headstages Thus we recommend to use always additional filters for signal conditioning version 2 5 page 15 HVC 03M User Manual 7 Technical Data HVC 03M CURRENT RANGE max luA in 100 MQ CURRENT OUT 100 nA V POTENTIAL OUT mV OFFSET
14. tentiometer for setting the capacity compensation of the electrode A the amplifier is controlled by SEC 03M labeled A OFF the amplifier is disconnected from all external signals and does not generate current The LED at the headstage does not light in OFF position B the amplifier is controlled by SEC 03M labeled B EXT the amplifier is controlled by an external signal e g for being used for iontophoresis Note If the control selector switch is in A or B position the respective SEC 03M amplifier cannot be operated as a single electrode amplifier in BRIDGE mode Only two electrode voltage or current clamp mode is possible 6 OFFSET potentiometer Potentiometer for OFFSET cancellation of the electrode Input BNC connector for an external signal By application of an external signal to this BNC connector and setting the control selector switch 5 to EXT the HVC 03M can be used as amplifier for iontophoresis Scaling 100 nA 1 V 8 HEADSTAGE connector Connector for the HVC 03M headstage version 2 5 page 8 HVC 03M User Manual 9 OUT BNC connector BNC connector providing the current signal of the high voltage headstage Scaling 100 nA 1 V or mV respectively 10 RS COMP potentiometer optional Optional only needed for very large currents gt 2 uA 11 Reet IceL VceL switch Switch for showing the electrode resistance in MQ Rcex for showing the electrode current in nA IceL
15. wer supply 2 4 PWR 03D The external power supply PWR 03D is capable of driving up to 3 EPMS E housings Each housing is connected by a 6 pole cable from the one of the three connectors on the front panel of the PWR 03D to the rear panel of the respective EPMS E housing see Figure 1 Figure 3 A POWER LED indicates that the PWR 03D is powered on see Figure 1 Power switch voltage selector and fuse are located at the rear panel see Figure 2 Note The chassis of the PWR 03D is connected to protective earth and it provides protective earth to the EPMS E housing if connected version 2 5 page 4 HVC 03M User Manual Pw R030 DRAN Giri b AMA a Figure 1 PWR 03D front panel view Figure 2 PWR 03D rear panel view Note This power supply is intended to be used with npi EPMS E systems only 2 5 System Grounding EPMS 07 The 19 cabinet is grounded by the power cable through the ground pin of the mains connector protective earth In order to avoid ground loops the internal ground is isolated from the protective earth The internal ground is used on the BNC connectors or GROUND plugs of the modules that are inserted into the EPMS 07 housing The internal ground and mains ground protective earth can be connected by a wire using the ground plugs on the rear panel of the instrument It is not possible to predict whether measurements will be less or more noisy with the internal ground and mains ground connected We reco
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