características del ensayo de fototoxicidad
Contents
1. TIPS FOR SUCCESS A AE A Dh oy i nME A P LIA AGE P 4 k as y OBE tee ae r ONSAS TM Ki yi IRPA ahar ro RE Abr F ERT A CELL BASED and READY TO USE CONCEPT FOR N VITRO TRANSPORT EVALUATION e UPON RECEIPT OF PREADYPORT ASSAY KIT e Remove the plastic bag containing PreadyPort plates from the outer and inner shipping boxes and place the bag in a safe place protected from vibration jarring and light e Store plates at 20 25 C CHANGING THE SHIPPING MEDIUM e To change the shipping medium please use recommended basal plates COSTAR ref 3526 for 24 wells and Millipore ref PSHT004S5 for 96 wells These plates are not provided e Conditions for liquefying the shipping medium of PreadyPort plates Temperature 37 C Time 3 4 hours do not exceed 4 hours e Pre warm PreadyPort medium at 37 C before use e Avoid touching the cell monolayer when handling PreadyPort plates e Use a 24 or 96 well manifold DRUMMOND ref 3 000 097 for 24 wells and ref 3 000 093 for 96 well to remove cell culture medium e Use a multichannel pipette to dispense cell culture medium e Never change the shipping medium on more than one plate at a time e The Shipping medium from both the apical and the basal compartments must be changed as follows PREADYPORT 24 well regular Plate PREADYPORT 24 well regular Plate Costar 3526 Costar 3526 e Remove liquefied shipping medium Shipping Culture medium med2. ium Tansport plate Add fresh MIDCKII medium Remove liquefied ei shipping medium 1 LI PREADYPORT plate While removing or replacing fluids keep the basal and apical compartments separate e Plates should be returned to the incubator 37 C immediately after medium has been replaced TEER MEASUREMENT e Avoid contacting the cell monolayer with the electrode WORLD PRECISION INSTRUMENTS ref STX100C for 24 wells and ref STX100M for 96 wells If the recommended electrode is used there will be no direct contact with cell monolayer e For optimal TEER results cell culture medium should not be changed for 48 hours prior to measurement e Taking multiple TEER measurements in a short period of time can alter cell monolayer stability We recommend leaving the plate in the incubator for a minimum of 20 minutes after carrying out TEER measurements For detailed instructions please read carefully PreadyPort User s Manual For more information please contact reagents advancell net support solvo com SOLVO Biotechnology n ae el a ech oer PWAN l _ yar u 2 Buda rs Fax 36 23 503 941 PARC CIENTIFIC DE A Se sala ean ie 10 12 ee E mail sales solvo com CYS U 08028 BARCELONA SPAIN oe eo A TEL 34 93 403 45 45 Zs FAX 34 93 403 45 44 Me advancell advancell net WANN GAIA CAM
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