Manual - Pipette.com
Contents
1. PRODUCT DESCRIPTION The VARICHANNEL DIGITAL MULTICHANNEL PIPETTE is a hand held adjustable volume and adjustable channel spacing micropipette for sampling and dispensing of accurate liquid volumes It operates on an air displacement principle i e an air interface and uses detachable disposable tips The VARICHANNEL can be assembled and adjusted into any configuration between 1 and 12 channel pipettes with variable tip spacing starting from a minimum of 9 mm A tip ejector system is provided adjusting automatically to the selected tip spacing The VARICHANNEL is available in three models covering a total volume range of 5 to 1000 ul The volume ranges are Volume range Scale Division 5 50 ul 0 5 ul 40 200 ul 1 0 ul 200 1000 ul 5 0 ul PIPETTE OPERATION DIGITAL DISPLAY Fig 2 The adjusted delivery volume is clearly indicated by a digital display on the pipette handle SETTING THE DELIVERY VOLUME Fig 3 The delivery volume is set using the operating button on the top of the pipette To increase the delivery volume turn the opera ting button counter clockwise and to decrease turn it clockwise Ensure that the desired delivery volume clicks into place and that the digits for the volume are completely visible on the handle display Do not try to set volumes by force outside the pipette s specified volume range TIP EJECTION Fig 4 Each pipette is fitted with a tip ejector system to help eliminate any contamina2. altered Recalibrate caused e g by according to misuse instructions Inaccurate Unsuitable Recalibrate dispensing X calibration with the liquids with certain Many high viscosity in question Conversion table Value of the conversion factor Z ul mg as a function of temperature and pressure for distilled water Temper Air pressure ature C hPA mbar 800 853 907 960 013 067 15 1 0018 0018 0019 0019 0020 0020 15 5 1 0018 1 0018 0019 0020 0020 0021 16 1 0019 1 0020 0020 0021 0021 0022 16 5 0020 0020 0021 0022 0022 0023 17 1 0021 0021 0022 0022 0023 0023 17 5 1 0022 1 0022 0023 0023 0024 1 0024 18 1 0022 0023 0024 1 0024 1 0025 0025 18 5 0023 0024 0025 0025 0026 0026 19 1 0024 1 0025 0025 0026 1 0027 0027 19 5 1 0025 1 0026 0026 1 0027 0028 0028 20 1 0026 0027 0027 0028 0029 0029 20 5 0027 0028 0028 1 0029 0030 0030 21 1 0028 1 0029 0030 0030 0031 0031 21 5 1 0030 1 0030 0031 0031 0032 0032 22 1 0031 0031 0032 0032 0033 0033 22 b 1 0032 1 0032 0033 0033 0034 1 0035 23 1 0033 1 0033 0034 1 0035 0035 0036 23 5 1 0034 0035 0035 0036 0036 0037 24 1 0035 0036 0036 0037 0038 0038 24 5 1 0037 1 0037 0038 0038 0039 0039 25 1 0038 1 0038 0039 0039 0040 0041 25 5 0039 0040 0040 0041 0041 0042 26 1 0040 0041 0042 0042 0043 0043 26 5 1 0042 1 0042 0043 0043 0044 1 00
3. to be dispensed 1 Depress the operating button all the way down to the se cond stop 2 Dip the tips just under the surface of the liquid in the reservoir 2 3 mm and slowly release the operating button This action will fill the tips Withdraw the tips from the liquid touching them against the edge of the reservoir to remove exess li quid 3 Deliver the preset volume by gently depressing the operating button to the first stop Hold the operating button at the first stop Some liquid will remain in the tip and should not be included in the delivery 4 Continue pipetting by repeating the steps 2 and 3 INSERTING THE VARICHANNEL SYRINGES Fig 8 9 To install or remove a cy linder piston combination follow the steps below 1 Open the lid at the end of the body 2 Depress the operating button all the way down 3 To remove a syringe just pull it out of the body by hold ing it at the tip cone 4 To insert a syringe is an opposite operation Be sure that the round piece at the end of the piston is in the right position see figures as well as the tip ejector square 5 Push the combination into its place taking care that each of the three parts goes into its appropiate grooves piston cylinder tip ejector square 6 Reinstall the lid ASSEMBLING THE TIP EJECTOR PUSHER Fig 11 To remove or install the tip ejector pusher first unscrew the small screw visible from the back of the handle see figure below M
4. 45 27 1 0043 0044 0044 1 0045 0045 0046 27 5 0044 0045 0046 0046 0047 0047 28 1 0046 1 0046 0047 0048 0048 0049 28 5 1 0047 1 0048 0048 0049 0050 0050 29 1 0049 0049 0050 0050 0051 0052 29 5 0050 0051 0051 0052 0052 0053 30 1 0052 1 0052 0053 0053 0054 1 0055 PT PISTON HEAD CROOVER CYLINDER GROOVER TIP EJECTOR SQUARE GROOVER x 1057490 orange m 99 1057500 yellow 1057510 blue qm Tip Ejector Spring Pusher 1131710 1058530 Handle 2202011 550 2202580 40 200ul 2202590 200 1000pl Locking part Ejector Bushing 1057620 1057800 Ejector Cover 1057810 Body Assembly 2203700 5 50ul 2203701 40 1000pl Piston Piston Piston Ben Hee Assembly Assembly Assembly 550 1 40 2000 200 1000 1057790 2203740 2203730 2203720 TIPINFO For volumes 5 50 ul use Finntip 250 Universal Finntip 200 Ext or Finntip 300 gt For volumes 40 200 ul use Finntip 250 Universal Finntip 200 Ext Tip Ejector Tip Ejector or Finntip 300 Square5 200ul Square 1000 ul For volumes 200 1000 ul use 1057840 1057830 Finntip 1000 or Finntip 1000 Ext SPARE PARTS Product specifications are subject to change without 8 pri
5. AINTENANCE When the FINNPIPETTE VARICHANNEL is not in use make sure it is safely stored in a vertical position We recommended a FINNPIPETTE stand for this purpose SHORT TERM CHECKING The pipette should be checked at the beginning of each day for dust and dirt on outside surfaces of the pipette Particular attention should be paid to the pipettes tip cones Solvents should not be used for cleaning the pipette 5 LONG TERM MAINTENANCE If the pipette is used daily it should be checked at least twice a year The servicing procedure is as follows 1 Open the lid on the body and remove all the syringes 2 Clean the pistons and O rings with a dry napless cloth and grease them with high vacuum silicone grease included in the package 3 Reassemble the pipette In the event of a malfunction send the pipette to nearest Labsystems representative Please enclose a complete list of liquids which have entered the pipette CALIBRATION All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with distillled or deionized water Normally the pipettes do not need adjustment but they are constructed to permit recalibration and adjustment for liquids of different temperature and viscosity Device requirements and test conditions An analytical balance must be used The scale graduation value of the balance should be chosen according to the selected test volume of the pipette Volume ran
6. FINNPIPETTE VARICHANNEL INSTRUCTIONS FOR USE Code Volume range 4347010 5 50 ul 4347020 40 200 ul 4347030 200 1000 ul OPERATING BUTTON GRIPPY TIP EJECTOR PUSHER HANDLE DIGITAL DISPLAY BODY COVER MOVABLE TIP CONES DISPOSABLE TIPS l Y d UV 1 Thermo Labsystems Thermo Labsystems Oy 100 FIN 01621 Vantaa Finland Tel 358 9 329 100 Fax 358 9 3291 0414 www finnpipette com A Thermo Electron Business o lt A o Lu o n F st Oo o O O O O LO 00 lt e sjeuueyo jo ON ui Buroedg ADJUSTING THE TIP CONE SPACING fig 1 Follow these steps 1 Press the operating button all the way down 2 Move and adjust the tip cones to the spacing required 3 Use the accompanying drawing for help to adjust the spacing accurately 4 The maximum spacing available with different number of channels is shown in the table below Number of Spacing Spacing channels 200 1000ul 5 200 ul 2 100 mm 100 mm 3 50 mm 50 mm 4 25 mm 25 mm 6 20 mm 20 mm 7 16 7 mm 16 7 mm 8 143 mm 14 3 mm 9 12 5 mm 12 5 mm 10 11 mm 11 mm 11 10 mm 12 9 mm Maximum spacing
7. ange Volume Accuracy Precision ul Hl sd ul cv 12 5 50ul 075 15 0 40 0 8 bul 025 50 0 13 25 12 40 2001 200 200 10 080 04 40u 180 45 0 60 1 5 10 200 1000u 100001 1200 1 2 6 00 0 6 200u 600 30 5 00 2 5 ADJUSTMENT Fig 10 Adjustment is done with the service tool 1 Place the service tool into the openings of the calibration nut at the top of the handle 2 Turn the service tool clockwise to increase or counterclockwise to decrease the volume 3 After adjustment check the calibration according to the instructions above FORMULAS FOR CALCULATING RESULTS Conversion of mass to volume V w e x Z V volume ul w weight mg e evaporation loss mg Z conversion factor for mg ul conversion Evaporation loss can be significant with low volumes To determine mass loss dispence water to the weighing vessel note the reading and start a stopwatch See how much the rading decreases during 30 seconds e g 6 mg 0 2mg s Compare this to the pipetting time from tareing to reading Typically pipetting time might be 10 seconds and the mass loss is 2 mg 10s x 0 2mg s in this example If an evaporation trap or lid on the vessel is used the correction of evaporation is usually unneccessary The factor Z is for converting the weight of the water to volume at test temperature and pressure A typical value is 1 0032 ul mg at 22 C and 95 kPa See the conversion table on page 9 Accuracy systematic err
8. ge readable graduation under 10 ul 0 001 mg 10 100 ul 0 01 mg above 100 ul 0 1 mg Test liquid Water distilled or deionized grade 3 water conforming ISO 3696 Tests are done in a draft free room at a constant 0 5 C temperature of water pipette and air between 20C to 25C The relative humidity must be above 55 Espoecially with volumes under 50ul the air humidity should ve as high as possible to reduce the effect of evanporation loss Special accessories such as the evaporation trap are recommended CHECKING CALIBRATION One of the middle channels of the pipette is checked with the maximum volume nominal volume and with the minimum volume or 10 96 of maximum volume whichever is higher E g Finnpipette 0 5 10 ul is tested at 10 ul and 1 ul A new tip is first pre wetted 3 5 times and series of ten pipetting are done with both volumes A pipette is always adjusted for delivery Ex of the selected volume Measuring volumes taken from balance is not allowed If the calculated results are in the limits the calibration of the pipette is correct Procedure 1 Do 10 pipettings with the minimim volume 2 Do 10 pipettings with the maximum volume 3 Caculate the accuracy A and precision cv of both series 4 Compare the results to the limits in the Table 1 If the results are in the limits of Table 1 then the calibration of the pipette is correct Otherwise the pipette must be adjusted and checked again Channel R
9. or Accuracy is the difference between the dispensed volume and the selected volume of a pipette A V V accuracy V mean volume V nominal volume Accuracy can be expressed as a relative value A 100 x A V Precision random error Precision refers to the repeatability of the pipettings It is expressed as standasd deviation s or coefficient of variation cv standards deviation S n V mean volume S gt V number of measurements 077 1 lt the relative value of standard deviation cv 100 xs v The FINNPIPETTE VARICHANNEL is shipped in a custom made expanded polystyrene package The package contains the following items 1 VARICHANNEL pipette 2 Instruction Manual 3 Tube of Lubric Grease 4 Samples of Tips 5 Service tool TROUBLE SHOOTING TABLE The following table is a guide to possible problems and their solutions Fault Possible reason Solution Leakage Tip incorrectly Attach firmly attached Foreign bodies Clean tip cones between tips and thoroughly and tip cones attach new tips Foreign bodies Clean and grease between the piston O rings and pistons and the O rings Use high vacuum in the cylinders silicone grease Insufficient amount Grease accordingly of grease on pistons and O rings O rings damaged Change the O rings Inaccurate Incorrect operation Follow instructions dispensing carefully Tips incorrectly Attach firmly attached Calibration
10. or notice Finnpipette and Finntip are registered 1 trademarks of Thermo Labsystems Oy
11. tion possibilities The tip ejector system consists of a set of tip ejector squares same amount as tip cones operating together with the ejector ribs and tip ejector plunger Direct the pipette towards a suitable waste receptacle and press the tip ejector plunger with your thumb The tips will be ejected cleanly PIPETTING TECHNIQUES FIGURES 5 7 A READY POSITION B FIRST STOP C SECOND STOP Ensure that clean tips are firmly pushed on to the tip cones of the pipette The operation of the pipette is controlled by the operating button For the best possible accuracy Operate the button slowly and with steady speed at all times particularly when working with liquids having high viscosity Never let the operating button snap back When working with serum or biological fluids rinse the tips with the liquid before pipetting This is done by filling and emptying the tips Hold the pipette vertically 10 degrees maximum from vertical during the liquid intake For maximum hand control hold the pipette tightly in the hand with the Grippy resting on the index finger FORWARD TECHNIQUE fig 5 Fill a clean reagent reservoir with the liquid to be dispensed 1 Depress the operating button to the first stop 2 Dip the tips just under the surface of the liquid in the reservoir 2 3 mm and slowly release the operating button Withdraw the tips from the liquid touching them against the edge of the reservoir to remo
12. ve exess liquid 3 Deliver the liquid by gently depressing the operating button to the first stop After a delay of about a second continue to depress the operating button all the way down to the second stop This action will empty the tips 4 Release the operating button to the ready position If necessary change the tips and continue with the pipetting REVERSE TECHNIQUE fig 6 The reverse technique is suitable for dispensing liquids having high viscosity or which have a tendency to foam easily The technique is also recommended for dispensing very small volumes Fill a clean reagent reservoir with the liquid to be dispensed 1 Depress the operating button all the way down to the se cond stop 2 Dip the tips just under the surface of the liquid in the reservoir 2 3 mm and slowly release the operating button This action will fill the tips Withdraw the tips from the liquid tou ching them against the edge of the reservoir to remove exess liquid 3 Deliver the preset volume by gently depressing the operating button to the first stop Hold the operating button at the first stop Some liquid will remain in the tip and should not be included in the delivery 4 The remaining liquid is either discarded with the tips or pipetted back into the container REPETITVE TECHNIQUE fig 7 The repetitive technique offers a rapid and simple procdure for repeated delivery of the same volume Fill a clean reagent reservoir with the liquid
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