Home

Hot Link to New Brunswick BioFlo 110 Operations Manual

1. PCU TERMINATOR PLUG 1273 8004 POWER CABLE 0720 2054 OPTIONAL CONTROL MODULES ie RS 485 CABLE L E M1273 8002 POWER E CONTROLLER NO eR Ens rd c 2 eJ aya EDS rou p 2 9 EEN ES J FOR ARE 5 485 m 7072052099 B M1273 8003 To connect the first stack to another 1 Use along RS 485 communication cable Connect it to either the topmost controller bus on the first stack 1 the upper connector on PCU or to the bottommost controller bus i e the lower connector on the Power Controller Save the terminator plug from the controller bus for reuse 2 Connect the other end to either the topmost or the bottommost controller bus on the next stack 3 Using short RS 485 cables continue to daisy chain each module to the next in the second stack as you did before Be sure that a terminator plug is on each unused controller bus connector Repeat steps 1 3 above for stacks three and four if appropriate CAUTION A terminator plug must be fastened on both unused Controller Bus connectors in the system for the communication links to work properly New Brunswick Scientific User
2. e et i Des reu 115 94 22 Vessel Cleaning RED R ee oU De ra Ae START 115 8 5 PORT amp ADAPTER O RING REPLACEMENT 116 8 6 HEADPEATE O RING REPEACEMENT terrere Pee e a 117 8 7 BEARING HOUSING CAP 117 8 8 REPEACINGPGU ione edo te eee Ware e e E ER 117 RID Ur P 118 9 1 FROUBLESHOOTING re i eee bee EE ee re e reete ta r e ea E 118 REPLACEMENT PARTS amp ACCESSORIES e eere ee ee eee seen eese eese see seta aset seen eese 119 10 1 REPLACEMENT PARTS EE PUE 119 10 2 REPLACEMENT VESSELS ei AlAe enean epe aede pee teccbeet cae ccc e ded 122 10 3 MISCELLANEOUS PARTS amp OPTIONAL 55 5 122 10 4 MODULE KITS n nea ctr eee etes teta Bekins eene ertet tees 123 APPENDIX SPECIFICATIONS siaviscsssscsscsescossccesscseccoscunccsssccescssseteossuuscossvecsdeessesssbusdeosooncsessebscseessbanssess 124 New Brunswick Scientific User s Guide 11 1 EE 124 11 2 PUMP EE OW RATES it Hetero
3. PCU TERMINATOR PLUG M1273 8004 POWER CABLE 0720 2054 OPTIONAL CONTROL MODULES ie RS 485 CABLE C 1273 8002 aka DAISY CHAIN n E POWER fel LESS HE J A cH EEN ES J bu RS 485 CABLE i 1273 8003 Figure 50 Thermal Mass Flow Controller Rear Panel Unit Address Selector Location Module Reference Serial Table Number for Unit REE Address Selector MUGO 24 volt DC input connector Indicator Lights RS 485 Connector E A Module Entry Cable T amp NOTE Be sure to check the setting of the Unit Address Selector on the rear panel to be sure it is the same as the other control modules for this system For details see Section 5 2 BioFlo 110 Modular Fermentor M1273 0054 User s Guide 145 With reference to Figure 51 below 1 If you have the Gas Mix module connect the Gas Mix module output to the TMFC Gas Input if you do not have the Gas Mix module connect your air supply to the Gas Input 2 Connect the TMFC Gas Output connector to the sparger inlet with the tubing provided Figure 51 TMFC Front View IN Gas Input Connector OUT Gas Output Connector 14 2 Loading Software Update If you purchased the optional TMFC module with the rest of your BioFlo 110 system the supervisory software is fully configured for i
4. 139 13 2 1 General Sp cifications aei eee 139 13 2 2 PHOLOCOL ERR RE 140 13 2 3 Requests amp Response iege e egisti ier 140 13 2 4 Change Commutids ia ie oH ette e OU ret ied ES S Ea N 141 13 2 5 Message ee 141 13 3 MODBUS COMMUNICATIONS 2 21 20 1 0000000000000000000 141 13 3 1 Register EA River aia cae ore 142 14 APPENDIX D THERMAL MASS FLOW CONTROLLFEXR 4 eee eese ee eese toes 0 143 14 1 INSTAEDBATION 5802 cash Ve 143 14 2 LOADING SOFTWARE 0 1 22202000000 00000000000000000000000000 145 14 3 OPERATION A 146 14 3 1 Fermentation Mode Setup eese ninisi i 147 14 3 2 Fermentation Mode Cascades eene enne enini te enses esee sete esse sese sete ense saeua 149 14 3 3 Cell Culture Mode Setup ee ter rre 150 14 3 4 Cell Culture Mode nnn nnns nsns ese ese ese ese ese ese ases eset ese s en 151 14 4 CLEANING E E ES EEE ET neptem died
5. 0 0 00 lt Pump B 00 00 J Manual lt Pump 0 0 0 0 Off lt pH 6 68 7 00pH Auto S dO2 77 1 0 0 96 Auto S lt The above screen is only one sample loop names will reflect the modules in your system 2 If you have more than one fermentor running off the PCU press the gt button again to open the Main Screen for Unit 2 see sample screen below 3 Press again for Unit 3 and again for Unit 4 BioFlo 110 Modular Fermentor M1273 0054 Name Value Setpoint Control Temp 257 30 0 C Auto Agit 253 350rpm Auto Pump A 0 0 0 0 96 Manual Pump B 0 0 0 0 96 Manual Pump C 0 0 0 0 96 Manual pH 6 59 7 00pH Auto dO2 81 2 0 0 96 Auto 4 Press once more to return to the Unit 1 labeled BioFlo 110 Main Screen User s Guide 89 7 4 Set the Temperature Loop All loops have three parameters that you can specify through the PCU They are the Control Mode Setpoint and Graphic Display As you go through the following steps to set the Temperature loop you will learn how to set the others as well 7 4 1 Control Mode To change the control mode of the temperature loop starting from the Main Screen see above of the appropriate fermentation unit 1 Press the selector button in the Temp row see sample screen below Press here Name Value Setpoint Control Temp 234 30 0 C Off Agit 134 350rpm Auto Pump A 0 0 0 0 96 Manual Pump B 0 0 0 0 96 Manual Pump C 0 0 0 0 96 Manua
6. 2 Press the Antifoam selector button The pump Details screen screen returns after a short delay the control mode will reflect your choice Note that a new parameter Probe Sensitivity also appears Pump C 0 0 96 Control Foam Control Probe Sens 75 0 Setpoint Disp Hi Disp Lo Sensitivity When the probe is activated CV 3 Probe sensitivity reflects the density of foam necessary to activate the antifoam m n 1 pump set sensitivity value we recommend starting with 75 0 press the either 0 T Sensitivity selector button input the desired value using the touchpad and the setpoint press the Enter key 4 Press the button to return to the Main Screen 5 Follow the same procedure to set the Wet On pump and the probe sensitivity value and the Wet Off pump and probe sensitivity New Brunswick Scientific User s Guide 112 7 14 Inoculation When you are ready to inoculate the media to start a batch observe aseptic techniques to do the following 1 Using a syringe inject the inoculum through the self sealing rubber seal of the septum port YT amp NOTE Always inspect the condition of the septum seal and the bearing housing cap prior to autoclaving Replace as necessary 7 15 Sterile Sampling To remove a sample from an active batch 1 Make sure the sampler syringe plunger is completely closed 2 Confirm that the sample bottle is in place and finger tight against its
7. 11 12 13 14 15 16 17 Grasp the fuse and pull it straight up until it separates from its base Insert a new fuse taking care to align its two pins with the pinholes in the base If you are replacing one of the heater fuses use a continuity meter to determine which fuse is blown Ease the cover back in place lowering it straight down until it fits tightly Take care to tuck the ground wire inside clear of both the cover and the lip of the base Reinstall the side mounting screws Tighten securely Reinstall the water solenoid valve assembly tightening the screws against the mounting plate Reconnect the motor drive RTD and heater to the front panel Reconnect the water solenoid valve to the rear panel Reconnect the Power Controller to the other module s Reconnect the power cord AN WARNING Always disconnect the power cord from the Power Controller and PCU before undertaking the following procedures 12 2 Replacing PCU Batteries Unplug all power cords from the system Disconnect the PCU from any other modules Unscrew the eight side mounting screws that hold the cover in place Retain them for reuse Carefully pull the cover straight up until it separates from the base Be careful not to pull on the ground wire that is attached to the front of the left side from the rear or on the ribbon cables that are attached to the front of the cover Rest the cover on its front panel ad
8. 3L vessels gt 10 cm to height of 7 5L and 14L vessels e Addition bottles add no height to vessels with condenser e Sampler adds approximately 4 cm to diameter 11 4 2 Non Jacketed Vessel Dimensions for Autoclaving Inclined 79 NOTE For use with an Angled Autoclave Rack the attachment of addition bottles is not recommended Non Jacketed Vessel Assembly with optional Angled Autoclave Rack M1273 9266 amp as prepared for autoclaving Total Vessel Volume 7 5L 14L Height with Exhaust Condenser 18 in 46 20 in 51 cm Length with Exhaust Condenser 28 in 71 cm 31 5 in 80 cm Width with Exhaust Condenser 11 5 in 29 cm 11 5 in 29 cm BioFlo 110 Modular Fermentor M1273 0054 User s Guide 129 11 4 3 Water Jacketed Vessel Dimensions for Autoclaving Vertical Water Jacketed Vessel Assembly as prepared for autoclavin Total Vessel Volume 1 3L 3 01 7 5L 14 0L Height without Exhaust Condenser 16 in 18 in 20 5 in 27 in to top of bearing housing 41 cm 46 cm 52 cm 69 cm Diameter without Exhaust Condenser 9 25 in 9 25 in 11 5 in 11 5 in 23 5 cm 23 5 cm 29 cm 29 cm Height with Exhaust Condenser 20 5 in 22 5 in 24 5 in 31 in to top of bent over exhaust filter 52 cm 57 cm 62 cm 79 cm Width with Exhaust Condenser 9 5 in 9 5 in 11 5 in 11 5 in 24 1 cm 24 1 cm 29 cm 29 cm Length with Exhaust C
9. 6 53 pH 2 Defaults lt lt 3 Deadband 0 20 Deadband lt A Output Multiplier 100 00 Multiplier lt J Advanced Control P Gain 140 00 PGain lt 14 00 Gain 67 1 2 Press the Multiplier selector button A highlighted text box opens pH 6 53 pH Defaults Deadband Deadband 0 20 Output Multiplier 100 00 Advanced Control P Gain 140 00 P Gain l Gain 14 00 oe Gain 3 Using the touchpad enter the desired Multiplier value note that you can set this percentage to the hundredths BioFlo 110 Modular Fermentor M1273 0054 User s Guide 97 6 53 Defaults Deadband 0 20 Deadband Output Multiplier 100 00 25 00 F Advanced Control P Gain 140 00 P Gain l Gain 14 00 Gain 4 Press the Enter key to save the new value or press X to cancel The new value appears onscreen 7 6 3 Setting amp Gains for pH Control XA NOTE We recommend that you do not change the factory P amp I settings unless you are an advanced user of P amp I algorithms P and I values are numbers that determine how the fermentor responds to changing growth conditions and new setpoints Because growth characteristics of bacterial and cell cultures are very different they require some different P and I values for proper control Incorrect P and I values can cause poor control which may actually mimic component failure For
10. Gas Lo Limit P Gain l Gain Cascade To Agit Hi Limit Agit Lo Limit Gas Hi Limit None 1000 250 20 0 5 0 0 05 0 25 Agit Gas Agit Gas None New Brunswick Scientific User s Guide 150 If you have with the Gas Mix Module the dO2 Cascade screen will look like this d dO2 73 1 Cascade To None Agit Hi Limit 1000 Agit Lo Limit 250 Gas Hi Limit 20 0 Gas Lo Limit 5 0 P Gain 0 05 l Gain 0 25 Agit Gas Gas O2 Agit Gas Ag Ga O2 None Make your choices as explained in Section 7 8 2 14 3 3 Cell Culture Mode Setup 1 In the main screen select the Gas loop The Gas loop screen opens 0 0 Control Off 1 Gas Mix Oft o Gas 2 0 0 1 2 0 0 0 O2 0 0 Air 0 0 SLPM Gas Flow 4 Gas Demand Off 10096 CO2 10096 N2 10096 O2 100 Air 2 While the Control mode and Gas Mix are Off select Air or any other gas to change the gas mix 3 Select Gas Flow then use the following screen to select a new gas flow Setpoint if so desired BioFlo 110 Modular Fermentor M1273 0054 User s Guide 151 m Gas 0 0 SLPM Control Off E Output 0 0 Control i Setpoint o Disp Hi Disp Lo 1 0 4 The default setpoint is 5 If you wish to change it select Setpoint then type in the desired value from 0 to 5 SLPM the system will automatically limit the gas setpoint to 5 5 If you wish to adj
11. pH 6 53 pH Defaults Deadband 0 00 0 20 D adba L Output Multiplier 100 00 Multiplier Advanced Control P Gain 140 00 P Gain l Gain 14 Gain 00 I Gain 4 Press the Enter key or press X to cancel The new value appears onscreen 7 6 2 Setting the Output Multiplier for pH Control Later when you begin your batch you may notice that pH remains steady at the setpoint yet the acid and base pumps are continually alternating in making additions This is an indication that the controller is overcompensating for minor fluctuations in pH Output Multiplier is a feature that attenuates controller output to the acid and base pumps and the CO gas line providing more nuanced control of additions to maintain pH We recommend that you begin by implementing a multiplier of 25 This means that if the controller s output to the base pump for example is 100 then the 25 multiplier will reduce pump output to 2596 If the controller s output to the pump is 50 the 25 multiplier factor will reduce pump output to 12 5 New Brunswick Scientific User s Guide 96 If after applying an Output Multiplier of 25 you find the results are attenuated but the controller seems unable to maintain the setpoint increase the Multiplier by small increments until the controller is able to maintain setpoint To set the Multiplier 1 In the pH Loop Details screen press the Setup selector button The pH Setup screen opens
12. eee 34 4 5 22 Install FCA Plates ss iud ote iei e OP OO HEURES 34 4 6 VESSEL ASSEMBLY WATER JACKETED 35 46A Headpl te iie etaed pee E e RR RUE 37 New Brunswick Scientific User s Guide 46 2 Install Headplate Clamping Ring micie re 40 4 6 3 Install V essel On Base Plate 4 epe E ee 40 4 6 4 Install Baffle 14 01 Fermentation Vessels 41 4 6 3 oe a ee ttes eee deer eub ei aede 41 46 0 Install Sparg y iios Ae ab De bib 42 4 6 7 Install Harvest Tube 43 4 6 8 Install Sampler Tube see e e repa eti ee a E e E titer PE Mai re iei 43 4 6 9 Install Thermowell 43 4 6 10 Install Foam Probe 43 4 6 11 Install Foam Exhaust Tube 9 44 46 12 Install Level Prove s cccccccsccccssecessssescecesssssscecusesesscecsseeesseeceseeesseecsaeessssecsseeessseceeessseeceeesesaeceeeeeesaees 44 4 6 13 Install Addition Tube s en ntes nennen 44 4 6 14 Install DELP re oie te 44 4 6 15 I
13. nnne 21 45 55 _ E a e Pre ER 22 4 5 6 Install Cooling Coil ice iiia ERE e UR IE EETAS Sea ipsia 23 4 5 7 Install Sparger 3 0L 7 51 amp 14 01 200220 00000000 23 4 5 8 Install Harvest Tube e nare tenete nenne nnn 24 ADO Jnstall Sampler Tube iie ee Caes e e eee HE RETE eg AR 24 4 5 10 Install Thermowell eeeeeeeeeeeeeeeeeeeeeeeeeeee nennen asas nsns aane ase 24 4 5 11 Install Foam Probe 24 4 5 12 Install Foam Exhaust 25 4 5 13 Install Level 2 25 4 5 14 Install Addition 25 4 5 15 Install pH Probe iati e err eee ie 25 4 5 16 Install dO2 Probe itae inan nien dtp rese 27 4 5 17 Install Exhaust nennen nnn nnn nnn nnn n nnn n nnne ese ese 28 4 5 16 Install Sampler 30 4 5 19 Install Foam Trap ratsia ceo DER eget eut eie ee de dert ee hee aeter eger Hs 32 4 5 20 Dlug Unused Ports nc D Re e teet nive ee esee ee ettet e e iir yy 33 4 5 21 Install 1 3L 3 0L or 7 5L Fermentation Vessel Baffle
14. pec bees emet ede Reo sR 126 11 3 EE 127 11 4 RA S E E EE 127 11 4 1 Non Jacketed Vessel Dimensions for Autoclaving Vertical eene 127 11 4 2 Non Jacketed Vessel Dimensions for Autoclaving 128 11 4 3 Water Jacketed Vessel Dimensions for Autoclaving Vertical eene 129 11 4 4 Non Jacketed Vessel Dimensions for Autoclaving Inclined eese 129 12 APPENDIX B TECHNICAL 0 131 12 1 REPLACING POWER CONTROLLER FUSES csssessccecececsessaccecececsensaccecececeensseaecececeesesnaececeescsensaaeaeeecs 131 12 2 REPLACING PCU BATTERIES aet iet eee este avete Boa vane RC UG ERROR Ger os Re es 132 12 3 VOLTAGE SELECTION Getreide a modena ete qt d aie eite add 133 12 4 CHANGING PEU FUSING exe ee 134 12 4 1 Converting from Single Fuse to Double Fuse esee 134 12 5 P amp ILCONTROL BOUATIONS 5 5 ceto ertet tet tue este REO a E dete Grube ONE EEA ER 136 12 5 1 DH sea o RISUS RR ee a mid qe nq Ha 136 12 5 2 9pm LIT C A 137 13 APPENDIX C COMMUNICATIONS 8 2 00 138 13 1 COMMUNICATIONS 8 138 13 1 1 AFS ModBus Pin 139 13 2 AFS BIOCOMMAND COMMUNICATIONS PROTOCOL
15. From PCU To Recorder Pin 2 Channel 1 Pin 1 Pin 3 Channel 2 Pin 5 Pin 4 Channel 3 Pin 7 Pin 6 Channel 4 Pin 8 Pin 9 Shield To choose assign the outputs to the loops of choice 1 Press O to open the Systems Details screen 2 Press the selector button next to Recorder The Recorder screen will open Recorder Recorder Status 3 Press the selector button for Output 1 A screen with eight available loops Output 1 Output 2 Output 3 Output 4 Off None None None None Status Out 1 Out 2 Out 3 Out 4 opens Recorder None Recorder Status Off Tem p U1 Agit U1 Output 1 None PumpA U1 Output 2 None PumpB U1 Output 3 None PumpC U1 Output 4 None pH U1 More Initially all of the Output are assigned to None New Brunswick Scientific User s Guide 74 4 Press the selector button next to the loop you wish to assign to Output 1 If you do not see the loop you want press the selector button next to More to see the next group of available loops In this example we have selected Temp Ul which is the Temperature loop from Unit 1 The previous screen returns with the selected loop now assigned to Output 1 Recorder Recorder Status Off Off On Output 1 Temp U1 Output 2 None Output 3 None Output 4 None 5 Press the selector button next to On to turn Output 1 on 6 Repeat steps 4 and 5 above to assign Output 2 Output 3 and Outpu
16. Where P is dimensionless and I is in min New Brunswick Scientific User s Guide 138 13 APPENDIX COMMUNICATIONS PROTOCOLS A CAUTION This section is provided FOR ADVANCED PROGRAMMERS ONLY Do not attempt to apply or change any of this information unless you are professionally trained in these matters Interventions by unskilled operators may severely compromise the operation of your BioFlo 110 system and may also void the warranty 13 1 Communications Settings The onboard computer has two RS 232 serial ports labeled Comm1 and Comm2 Both ports are connected to the keyboard interface board KIB which converts them to RS 485 Commz1 is set up for connection to an NBS AFS BioCommand supervisory software interface box See also Section 13 2 Comm1 can also be used for connection to a computer equipped with an RS 485 ModBus port This allows the operator to control the BioFlo 110 with the remote computer See also Section 13 3 Comm1 Settings For AFS Use For ModBus Use Baud Rate 9600 19200 Data Bits 8 8 Stop Bits 2 1 Parity Even None Comm2 is set up to communicate with the other BioFlo 110 control modules which daisy chained together Terminators must be installed on any and all unused connectors Comm Settings Baud Rate 38400 Data Bits 8 Stop Bits 1 Parity None BioFlo 110 Modular Fermentor M1273 0054 User s Guide 139
17. 13 1 1 13 2 13 2 1 AFS ModBus Pin Connections The AFS ModBus 25 pin D connector on the rear panel is pinned out as follows Pin Signal Observations Number 13 RS422 Tx 25 RS422 Tx 24 RS422 Rx 12 RS422 Rx 7 Ground 2 RS232 Tx 3 RS 232 Rx 21 Mode Open for RS232 e Connect to 7 for RS422 AFS BioCommand Communications Protocol This RS422 communications protocol specification is for Slave equipment Slave refers to any instrument that is interfaced to the NBS BioCommand also called AFS system and Master refers to the BioCommand supervisory interface module General Specifications All line drivers and receivers must conform to IEEE RS 422 or RS 485 standards Communications settings are listed in Section 13 1 above There is no CTS RTS or XON XOFF handshaking The Master initiates all transactions A Slave transmit driver must be held in a high impedance state tri stated at all times except when it is responding to a transaction initiated by the Master All transactions are carried out sequentially one at a time All transactions are prefixed with a single byte that indicates the multidrop ID of the Slave station involved Valid Slave unit ID numbers 0 through 99 All transmissions from the Master are terminated with a carriage return character Al responses to data requests are terminated with a carriage return ODH char
18. 16 4 5 Vessel Assembly Non Jacketed One of the most versatile features of the BioFlo 110 is the wide variety of glass vessels available There are two types of vessels non jacketed which are provided with the basic models and water jacketed Each type of vessel is available in four sizes 1 3 liters 3 0 liters 7 5 liters and 14 0 liters total volume for more detail see Specifications in Appendix A If you have a water jacketed vessel skip to Section 0 Every single walled non jacketed vessel comes with a stainless steel stand from which the vessel is suspended The stand has four rubber feet to provide stability An electric heat blanket provides temperature control for the contents of the vessel The blanket has two large viewing windows so the culture remains visible for inspection See Figure 10 Figure 10 below shows a typical installation of the non jacketed vessel in its vessel stand with the most commonly used accessory equipment To provide a full view of how the internal components are arranged the heat blanket is not shown BioFlo 110 Modular Fermentor M1273 0054 User s Guide 17 Figure 10 Non Jacketed Vessel Assembly HEAT BLANKET BEARING AGITATION __ FED MOTOR x HOUSING 2 EXHAUST EXHAUST HEADPLATE M CLAMPING bw RING que BAFFLE LIFTING y HANDLE COOLING COIL hides Sparger THERMOWELL o U
19. 4 6 8 Install Sampler Tube 1 Working from beneath the headplate install the optional sampler tube in the sample port see Figures 22 23 amp 24 for reference If you are using the 1 3L vessel the sampler tube and harvest tube are welded into the same tri port to Save space 2 Finger tighten the knurled adapter on the sampler tube then use the Allen key provided to tighten the set screw 4 6 9 Install Thermowell 1 Working from above the headplate insert the thermowell tube into the RTD port see Figures 22 23 amp 24 for reference AX CAUTION Make sure that the thermowell does not touch the cooling coil 2 Finger tighten the knurled adapter on the thermowell 4 6 10 Install Foam Probe If you are using a foam sensor with a foam trap kit 1 Working from above the headplate insert the foam sensor into the appropriate port see Figures 22 23 amp 24 for reference 2 Finger tighten the knurled adapter New Brunswick Scientific User s Guide 44 4 6 11 Install Foam Exhaust Tube If you are using a foam trap install the foam exhaust tube 1 Working from beneath the headplate insert the foam exhaust tube into the appropriate port close to a headplate clamping nut see Figures 22 23 amp 24 for reference where you will later mount the foam trap Finger tighten the knurled adapter If you need to raise or lower the tube at any time use the Allen key provided to adjust the adapter s set scr
20. 6 Raw float 320 322 324 382 320 N Loop 2 7 Cal_gain 384 386 388 446 384 N Loop 2 8 Zero std off 448 450 452 510 448 N Loop 2 9 Eng units 512 516 520 636 512 N Loop 4 10 Raw input 642 643 644 673 642 N Loop 11 Cal offset 676 677 678 m 707 676 N Loop 12 Loop name 740 756 772 x 1236 740 N Loop 16 13 Control mode 1268 1276 1284 i 1516 1268 N Loop 8 79 NOTE Parameters 1 through 8 use two registers each Parameter 9 uses four registers Parameters 10 amp 11 use one register each Parameter 12 uses sixteen registers and Parameter 13 uses eight registers BioFlo 110 Modular Fermentor M1273 0054 User s Guide 143 14 APPENDIX D THERMAL MASS FLOW CONTROLLER The Thermal Mass Flow Controller TMFC replaces the rotameter The TMFC will give you the ability to display gas flow rate on the PCU This digital gas flow rate can also be logged to data acquisition software such as BioCommand Lite or BioCommand Plus When you use it with the dO2 pH Controller you can automatically adjust the gas flow into the system using either direct operator interaction setpoint manipulation or cascade control from the DO loop YT amp NOTE In Section 1 of this manual you read that the PCU serves as the operator interface for one to four vessels Because the PCU cannot support more than a total of 16 modules adding the Thermal Mass Flow Controller limit
21. SAMPLER BOTTLE HEADPLATE CLAMPING NUT SAMPLER TUBE HEADPLATE CLAMPING SCREW New Brunswick Scientific User s Guide 32 4 5 19 10 11 Connect a length of silicone tubing to the sampler tube on the headplate Secure it in place with a plastic tie Slip a thumb clamp onto the tubing Connect the other end of the tubing to the tall sampler inlet pipe Secure it in place with a plastic tie Connect a short length of silicone tubing to the short sampler outlet pipe Secure it in place with a plastic tie Connect the sterile syringe filter to the other end of the tubing taking care to respect the direction of flow if stamped on the filter Secure the tubing in place with a plastic tie Insert the tip of the sampler syringe as far as it will go into the open end of the filter Although the syringe will lodge there and hang freely in place you can add a plastic tie for security Close the plunger Remove the cap from one of the sample bottles and screw the bottle into the metal holder Position the entire assembly to your satisfaction then finger tighten the clamping nut Install Foam Trap If you are using a foam trap kit see Figure 20 below l Unscrew the headplate clamping nut or base clamping nut if you prefer to mount the trap at the base of the vessel closest to the foam exhaust tube Mount the foam trap bottle holder on the clamping screw using the hole at the end of the holder s
22. TEFLON O RING white PORT O RING black With reference to Figure 15 above 3 Gently slide the top portion of the knurled port adapter part of the probe kit onto the probe 4 Slide the two white ferrules onto the probe the narrower one on top of the deeper cup shaped one 5 Gently slide the bottom portion of the port adapter onto the probe taking care to orient the longer threaded section toward the top of the probe 6 Remove the two O rings installed in the pH port first slide the white Teflon O ring onto the probe then follow with the black 12mm port adapter O ring 7 Do not yet close up all the elements of the port adapter 8 Gently insert the probe into the appropriate port see Figures 11 12 amp 13 for reference allowing the O rings to seat fully into the port YT amp NOTE The fit may be snug Gently rotate the probe as you press it into the port to avoid breakage BioFlo 110 Modular Fermentor 1273 0054 User s Guide 27 9 Finger tighten the bottom portion of the port adapter into the port 10 Adjust the probe to the desired height then nesting the ferrules close the top portion of the adapter onto the bottom portion 11 Finger tighten the knurled adapter assembly 4 5 16 Install dO2 Probe 79 NOTE Prior to installation any dissolved oxygen probe you are using should be inspected for damage and replaced if necessary 7 NOTE To avoid damage to the probes during operation
23. The PCU comes with two Module Bus terminator plugs that cap its 9 pin D connectors on the rear panel As you plug RS 485 ModBus cables into these connectors move the terminator plugs to the open end of the cables to close them off To connect the modules to each other with the modules stacked in the preferred order 1 Use a short RS 485 communication cable provided to connect the Power Controller from its top controller bus 9 pin connector to the bottom controller bus connector of the next module above When you remove the terminator plug from the connector set it aside for reuse 2 In similar fashion continue to daisy chain each module to the next using the additional RS 485 cables provided 3 Cap off the final module s unused controller bus connector with the terminator plug You can interconnect as many as three additional module stacks each with its own Power Controller Through this interconnection all stacks will communicate with the PCU giving it control over all four associated vessels See Figure 8 on the following page for a sample installation BioFlo 110 Modular Fermentor M1273 0054 User s Guide 13 Figure 8 Interconnecting the Modules
24. s BioCommand communications protocols Optional BioCommand software adds automatic data logging centralized monitoring and control and process programming capabilities to one or more fermentors 1 4 Enhancing Your Fermentor Other accessories are available and new ones will be added to extend the capabilities of the BioFlo 110 Whenever your bioprocessing needs or applications change contact your New Brunswick Scientific sales representative for a current description of BioFlo 110 products BioFlo 110 Modular Fermentor M1273 0054 User s Guide 2 INSPECTION amp UNPACKING 2 1 Inspection of Boxes After you have received your order from New Brunswick Scientific inspect the boxes carefully for any damage that may have occurred during shipping Report any damage to the carrier and to your local NBS Sales Order Department immediately 2 2 Packing List Verification Unpack your order saving the packing materials for possible future use Also be sure to save the User s Guide for instruction and reference Verify against your NBS packing list that you have received the correct materials and that nothing is missing The part numbers on the packing list correspond to part numbers and or manufacturing numbers You will find the number affixed to each item either on a paper sticker on the packaging or on the serial number identification sticker on the equipment itself If any part of your order was damaged during shipping or i
25. 1 3L amp 3 0L M1273 9290 Pitched Blade 7 5L amp 14 0L M1230 9212 Spin Filters see following page Motors NBS Part Number Direct Drive Agitation Motor Cell Culture all vessels M1273 3135 Direct Drive Agitation Motor Fermentation 1 3L amp 3 0L M1273 3120 Direct Drive Agitation Motor Fermentation 7 5L amp 14 0L M1273 3125 Magnetic Drive Agitation Motor all vessels M1273 3130 BioFlo 110 Modular Fermentor 1273 0054 User s Guide 121 Spin Filters NBS Part Number Spin Filter Suspension Cells 1 3L M1273 3201 3 0L M1273 3202 7 5L M1273 3205 14 0L M1273 3210 Spin Filter Microcarriers 1 3L M1273 3211 3 0L M1273 3212 7 5L M1273 3215 14 0L M1273 3220 Tubing NBS Part Number Blue Polyurethane Tubing inch 3 2 ID 0740 3111 6 35 mm OD 50 feet White Silicone Tubing 3 16 inch 4 8 mm ID 5 16 inch P0740 2505 7 9 mm OD 25 feet White Silicone Tubing 1 16 inch ID 1 6 mm 3 16 inch P0740 2396 4 8 mm OD 50 feet White Braided PVC Tubing inch 6 35 mm ID 0 438 P0740 1631 inch 11 mm OD 15 feet Probes amp Sensors NBS Part Number dO2 Probe Kit Ingold polarographic 1 3L M1273 9974 dO2 Probe Kit Ingold polarographic 3 0L M1273 9879 dO2 Probe Kit Ingold polarographic 7 5L M1273 9886 dO2 Probe Kit Ingold polarographic 14 0L M1273 9888 dO2 Prob
26. 2 Carefully lower the headplate easing all of its attachments into the vessel without hitting the glass walls or the baffle inside if this is 14 01 fermentation vessel 79 NOTE If you are using a baffle after installing the headplate as indicated below insert any convenient length of wood plastic or stainless steel do not use any other kind of metal through open ports to push the baffle down as far as it will go The baffle is stainless steel repeated installations may cause it to retain a compressed position Expand it before you squeeze it for installation so it will spring back against the vessel walls 3 Align the headplate holes with clamping ring holes then slide it down until it rests securely against the vessel flange 4 Finger tighten each clamping screw a little at a time to secure the headplate working diagonally from one to another rather than working around the circle to apply equal pressure AN CAUTION To avoid vessel stress cracks especially during autoclaving never overtighten vessel clamping screws 4 7 Install the Agitation Motor 1 Make sure the motor coupling O ring is lubricated 2 Position the motor sleeve on the bearing housing see Figure 33 taking particular care to align the side slots with the shoulder studs as you ease the sleeve down until it rests securely on the bearing housing You may have to walk it down by rotating it slightly left and right BioFlo 110 Modular
27. Albans Herts ALA 0JJ United Kingdom Tel 44 0 1727 853855 or 0800 581331 Fax 44 0 1727 835666 E mail bioinfo nbsuk co uk Web www nbsuk co uk User s Guide WARNING High voltage Always make sure this equipment is properly grounded A WARNING This product is not designed to contain gases within the range of their lower explosion limit LEL and their upper explosion limit UEL If your process requires or produces gases be sure to verify their LEL and UEL concentration range available online AN CAUTION This equipment must be operated as described in this manual If operational guidelines are not followed equipment damage and personal injury can occur Please read the entire User s Guide before attempting to use this unit Do not use this equipment in a hazardous atmosphere or with hazardous materials for which the equipment was not designed New Brunswick Scientific Co Inc NBS is not responsible for any damage to this equipment that may result from the use of an accessory not manufactured by NBS BioFlo 110 Modular Fermentor M1273 0054 User s Guide Copyright Notice New Brunswick Scientific Company Inc Box 4005 44 Talmadge Road Edison New Jersey 08818 4005 Copyright 2007 New Brunswick Scientific Co Inc All Rights Reserved Reproduction adaptation or translation without prior written permission from New Brunswick Scientific is prohibited Disclaimer Notice New Brunswick
28. DRAWINGS 15 1 Module Schematics Figure 52 PCU Schematics d i 11 BioFlo 110 Modular Fermentor 1273 0054 User s Guide 155 Figure 53 Power Controller Schematics RFI FILTER ASSY PCB FILTER New Brunswick Scientific User s Guide 156 Figure 54 dO2 pH Controller Schematics DO pH P C B 1 BRACKET ASSEMBLY 1273 5045 DO2 2 MICRO CONTROLLER 1273 7000 SECTION A 5 6 2 3 7 9 4 1 5 6 2 7 9 4 GND SERIAL 1 0 SERIAL 1 0 12V THESE ARE THE 9 CONNECTORS MOUNTED ON THE 12V MICRO CONTROLLER TESE P C B 1273 7000 E o 1 GND SERIAL 1 0 SERIAL 1 0 412V i 12V 1 12V BioFlo 110 Modular Fermentor 1273 0054 User s Guide 157 Figure 55 Level Controller Schematics FOAM 2 2 PROBE FPWwMZ O 2 5 u PROBE J2 LEVEL CONTROL re 1273 7001 7 Foam 2 J1 PROBE 17 7 8 9 9 PIC MICRO CONTROLLER P C B 10 M1273 7000 25 29 SECTION A SECTION B _ secmon s 1 5 6 2 3 7 9 4 1 5 6 2 5 7 9 4 bi vai 71 GND i GND GND SERIAL 1 0 SERIAL 1 0 12V i i THESE ARE THE 9 12 T CONNECTORS MOUNTED ON THE 12V MICRO CONTROLLER 1 Zj 1273 7000 GND GND SERIAL 1 0 1
29. PCU 135 48 Fuse Block Cover Assembly PCU 135 49a Double Fusing Arrangement PCU 136 49b Single Fusing Arrangement PCU 136 50 Thermal Mass Flow Controller Rear Panel 144 51 Thermal Mass Flow Controller Front View 145 52 PCU Schematics 154 53 Power Controller Schematics 155 54 dO2 pH Controller Schematics 156 55 Level Controller Schematics 157 56 Gas Mix Controller Schematics 158 57 4 Pump Module Schematics 110V 159 58 4 Pump Module Schematics 220V 160 59 Thermal Mass Flow Controller Schematics 161 New Brunswick Scientific User s Guide 164 16 APPENDIX F PORTS amp ADAPTERS One of the flexibility features of the BioFlo 110 is the variety of adapters that allow you to set up the headplate to suit your needs The summary below will help you determine any adjustments you may wish to make to the recommended headplate arrangement 12mm amp 6 35mm diameters PORT SIZE APPLICATION PART NUMBER PLUGS 6 35mm Unused port M1273 9405 12mm Unused port M1273 9406 19mm Unused port M1273 9407 INSERTS 12mm Septum M1273 3031 12mm Thermowell RTD M1273 9200 1 3L M1273 9201 3 0L M1273 9202 7 5L M1273 9203 14 0L 12mm Tri port M1273 9961 SET SCREW ADAPTERS CAUTION Never use a set screw on a probe 12mm port to 12mm tube For Exhaust Tube or Condenser M1273 5058 6 35mm port to 6 35mm For Sparger Dip Tube Harvest M1273 5054 tube Cooling Coil 12mm port t
30. The screen reflects your choice in this case CO2 New Brunswick Scientific User s Guide 108 d Gas Mix 4 Gas Control 2 i Off E Gas 96 CO2 100 0 10096 CO2 N2 0 0 10096 N2 0 O2 0 0 10096 O2 Air 0 0 100 Air NOTE The gas will continue to feed at 100 until you manually select another gas return to automatic 4 Gas Mode to Demand mode or choose Off 7 NOTE If at any time after establishing a dO2 cascade you turn the Gas Mix controller Off using the Gas Mix screen the cascade will be overridden 7 12 Setting the Pumps 7 12 1 Pump Operation The BioFlo 110 supports five peristaltic pumps On the rear panel of the Power Controller the pump outlets labeled Acid and Base are integrated with the pH control loop This is why they do not appear as individual pumps on the Main Screen Assignable pumps A B and C do appear individually on the display Each of these can be operated manually by assigning a setpoint or be set to respond automatically to signals from conduction probes 7 12 2 Pump Setpoint and Multiplier Each of the three assignable pumps A B amp C accepts a setpoint The setpoint determines the percentage of On time during each ten second period while the pump is set to On For example if Pump has a setpoint of 40 every ten seconds it will turn on for four seconds Instead of a setpoint the Acid and Base Pumps use a Multiplier value which can be assigned i
31. s Guide 14 4 4 Important Warnings Before you begin to assemble or operate your vessel whether it is non jacketed or water jacketed be sure to read this section for it contains essential information cautions and warnings to protect your safety and the safety of your equipment A WARNING NEVER PRESSURIZE A GLASS VESSEL Always use eye protection and exercise caution in the vicinity of glass If the vessel exhaust becomes blocked pressure can build up possible shattering the vessel and endangering personnel As soon as you open the airflow valve s verify by feel that air is flowing freely from the exhaust If not immediately close the valve s or turn off the air gas supplies Never intentionally block the exhaust to raise vessel pressure Use the minimum air gas pressure that will provide adequate airflow for the application Never exceed the maximum air pressure of 10 psi This maximum pressure is necessary only to obtain the highest gas flow rates Itis capable of breaking the vessel if the vessel is not properly vented CAUTION To protect the integrity of your glass vessel and to avoid damage familiarize yourself with these cautions Never allow hot glass to touch cold water or a cold surface Never rest the vessel on an uneven surface Never drag or roll the vessel across any surface Avoid metal to glass contact With the exception of occasional contact with baffles inside a vessel used for fermentation
32. s Guide 70 The address is set using the unit address selector switches on the back panel of each module The module is shipped with all four switches On facing down As you can see in the reference table below which is also posted on the back panel of each module next to the unit address switches this is the address for Unit 1 the first vessel and its associated control modules 2 CAI f Make sure that each module is set to the same address for Unit 1 A unit address switch can be flipped up or down with a pencilpoint or the tip of a screwdriver If you have a second stack with a second vessel set those modules for the Unit 2 address Do the same for Units 3 and 4 if present 5 3 For PC Supervision 5 3 1 Connect to Supervisory System Connect the AFS ModBus 25 pin connector on the PCU using the appropriate cable to the PC s serial port Be sure to consult the user s manual that accompanies the supervisory software program and any interface box or converter you may need to complete the communications link The AFS ModBus connector pin outs are assigned as follows for RS 232 or RS 422 input and output Pin Signal 2 RS 232 Transmit Data 3 RS 232 Receive Data 7 RS 232 RS 422 Ground 21 RS 422 Enable Jumper to Pin 7 to enable RS 422 13 RS 422 Transmit Data 25 RS 422 Transmit Data 2
33. 0L Vessels T 3 16 SILICONE TUBING N SAMPLER PORT THUMB CLAMP _ duh SYRINGE 0 Th HEADPLATE SYRINGE FILTER SAMPLER BOTTLE HOLDER SAMPLER BOTTLE HEADPLATE CLAMPING NUT SAMPLER TUBE HEADPLATE CLAMPING SCREW 3 Connect a length of silicone tubing to the sampler tube outlet on the headplate Secure it in place with a plastic tie 4 Slipathumb clamp onto the tubing 5 Connect the other end of the tubing to the tall sampler inlet pipe Secure it in place with a plastic tie 6 Connect a short length of silicone tubing to the short sampler outlet pipe Secure it in place with a plastic tie 7 Connect the sterile syringe filter to the other end of the tubing taking care to respect the direction of flow if stamped on the filter Secure the tubing in place with a plastic tie 8 Insert the tip of the sampler syringe as far as it will go into the open end of the filter Although the syringe will lodge there and hang freely in place you can add a plastic tie for security Close the plunger 9 Remove the cap from one of the sample bottles and screw the bottle into the metal holder 10 Position the entire assembly to your satisfaction then finger tighten the clamping nut New Brunswick Scientific User s Guide 52 4 6 18 Install Foam Trap If you are using a foam trap kit see
34. BIOFLO 110 LIQUID ADDITIONS PUMP DOOR p EV uy PUMP UNIT NUMBER INDIVIDUAL PUMP ON OFF SWITCH Place the pump module adjacent to the vessel and its Power Controller Install the tubing according to the instructions in the pump manual You may want to consult the Pump Flow Rates listed in Section 11 2 The Power Controller also provides power to the peristaltic pumps Plug the pumps into the back panel of the Power Controller see Figure 2 1 The pumps used for pH control have dedicated sockets labeled Acid and Base 2 Other pumps can be plugged into the sockets labeled Pump A Pump B and Pump CAUTION Plug the pump cables ONLY into power controller sockets marked Pump DO NOT use the socket marked PCU For further details see Section 7 12 Setting the Pumps 4 10 5 Air and Optional Gases gases should be medical grade Gas pressure should not exceed 10 PSIG Be sure to secure all tubing connections with plastic ties If you are using the optional Air Filter Regulator kit see Section 10 3 install it according to the kit instructions BioFlo 110 Modular Fermentor M1273 0054 User s Guide 63 4 11 Without 4 Gas controller 1 2 3 Connect pressure regulated clean air to the inlet port of the rotameter using 5 16 inch 7 9 mm O
35. Control P Gain 0 20 P Gain l Gain 1 00 Gain Press the Cascade selector button The Cascade screen opens New Brunswick Scientific User s Guide 104 dO2 73 1 96 Cascade To None Agit Agit Hi Limit 300 4 Gas Agit Lo Limit 25 Demand None Advanced Control P Gain 0 20 l Gain 1 00 9e Press the Agit selector button The Setup screen returns showing the new cascade parameter J dO2 73 1 A Defaults F Cascade To Agit Cascade Hi Limit 300 Agit Hi Agit Lo Limit 25 Agit Lo 1 1 Advanced Control P Gain 0 20 P Gain 1 00 Gain If you wish to change the high and low limits for Agitation speed use Agit Hi and Agit Lo selector buttons to open an edit box for each use the touchpad to input a value and press the Enter button to save the new value to memory The Hi Limit may not exceed 300 and the Lo Limit may not go below 25 10 Press X to return to the dO2 Details screen or 1 to return to the Main Screen To cascade to 4 Gas follow steps 1 4 above but select 4 Gas instead of Agitation To cascade to Demand follow steps 1 4 above but select Demand instead of Agitation BioFlo 110 Modular Fermentor M1273 0054 User s Guide 105 79 NOTE After Cascade To Gas Mix or to Cascade Demand has been selected if the Gas Mix function is turned off through the 2 Loop Gas Mix screen the cascade will be overridde
36. Figure 32 below 1 Unscrew the headplate clamping nut or base clamping nut if you prefer to mount the trap at the base of the vessel closest to the foam exhaust tube 2 Place a washer on the clamping screw then mount the foam trap bottle holder on the screw using the hole at the end of the holder s mounting arm 3 Secure the holder in place with the clamping nut Leave the nut loose enough to swivel the holder 4 Firmly place the foam trap bottle 250 ml or 500 ml in the holder 5 With the bottle cap in place aseptically install a sterile 0 2 u filter on the shorter tube that penetrates the cap Be sure to respect the proper flow direction if stamped on the filter Figure 32 Foam Trap STERILE FILTER E FOAM TRAP BOTTLE 250 or 500 FOAM EXHAUST TUBE HEADPLATE m VESSEL 6 Connect a length of silicone tubing to the longer tube in the other bottle cap penetration Secure the tubing with a plastic tie and clamp it off on the top BOTTLE HOLDER BOTTLE HOLDER MOUNTING ARM HEADPLATE CLAMPING NUT BioFlo 110 Modular Fermentor M1273 0054 User s Guide 53 7 Connect the tubing securing it with a plastic tie to the foam exhaust tube in the headplate 8 After autoclaving you will position the bottle holder where you want it then finger tighten the clamping nut 4 6 19 Instal
37. Guide 126 BioFlo 110 Antifoam Level Controller Weight 4 Ib 1 8 kg Dimensions Height 4 in 10 2 cm Depth 12 in 30 5 cm Width 12 in 30 5 cm allow 3 inches 7 6 cm more front and rear for cable access Inputs Three 3 pin DIN Operating Modes connectors for Antifoam additions conduction Wet ON pump turns on when probe is wetted probes Wet OFF pump turns off when probe is wetted Power 12 VDC supplied by PCU BioFlo 110 Four Pump Module Weight 14 Ib 6 4 kg Dimensions Height 12 in 30 5 cm Depth 12 in 30 5 cm Width 12 in 30 5 cm allow 3 inches 7 6 cm more front and rear for cable access Pumps Four peristaltic type pumps independently powered Power Line voltage as labeled supplied through Power Controller BioFlo 110 Gas Mix Controller Weight 5 Ib 2 3 kg Dimensions Height 4 in 10 2 cm Depth 10 in 25 4 cm Width 10 in 25 4 cm allow 3 inches 7 6 cm more front and rear for cable access Range 0 100 Valves 4 solenoid valves Inputs 4 gas inputs oxygen air carbon dioxide and nitrogen Output 1 gas stream output to sparger Power 12 VDC supplied by PCU Maximum Gas 10 PSI Pressure 11 2 Pump Flow Rates The following table lists pump flow rates according to tubing size pump frequency and rotation speed Tubing
38. M1273 9842 For 7 5L or 14 0L Vessel M1273 9843 Level Controller only M1273 3103 Gas Mix Controller NBS Part Number Kit contains gas mix controller module entry cable polyurethane tubing 2 cable guides M1273 3104 4 Pump Module NBS Part Number Kit contains the pump housing box 4 peristaltic pumps power line 2 cable guides 100 120V M1273 3106 200 240V M1273 3116 Thermal Mass Flow Controller Kit contains the thermal mass flow controller module entry cable 24VDC power line with transformer polyurethane tubing 2 cable guides NBS Part Number Fermentation application 20 SLPM M1273 3109 Cell Culture application 5 SLPM M1273 3112 New Brunswick Scientific User s Guide 124 11 APPENDIX A SPECIFICATIONS 11 1 Modules BioFlo 110 PCU Weight Overall 5 Ib 2 3 kg Dimensions Height 6 in 15 3 cm Depth 13 in 35 cm Width 12 in 30 5 cm allow 3 inches 7 6 cm more front and rear for cable access Display 3 4 x 4 5 86 4 mm x 114 3 mm electroluminescent monochrome bright white on blue screen 74 VGA resolution Control Loop Name Display Range Control Modes Agitation 0 1200 rpm Off Automatic e Per vessel fermentation up to four 0 300 rpm cell vessels total culture Temperature 0 70 0 C Off Automatic e Upto 32 pH with dO2 pH Module 2 12 0 p
39. Number RS 485 Connectors daisy chain to other modules Indicator Lights New Brunswick Scientific User s Guide 4 1 4 Level Controller Figure 4 Level Controller Rear Panel CONTROLLER BUS Serial Number RS 485 Connectors daisy chain to Indicator Module Serial other modules Lights Number Unit Address Selector Location Reference Table for Unit Address Selector 4 1 5 Gas Mix Controller Figure 5 Gas Mix Controller Rear Panel CONTROLLER BUS Serial Number RS 485 Connectors daisy chain to Indicator Module Serial other modules Lights Number Unit Address Selector Location Reference Table for Unit Address Selector BioFlo 110 Modular Fermentor M1273 0054 User s Guide 4 1 6 Pump Module A four pump module is available to stack with the other BioFlo 110 modules or to stand freely adjacent to the module stack and vessel 79 NOTE Unlike the other modules the pump module is wired for either 110V 220V electric service only Be sure that your module is correct for your electrical supply Refer to Figure 6 below to locate the following feature POWER INLET PLUGS Located on the rear panel Each pump is individually connected to the Power Controller by cables provided Figure 6 4 Pump Module Rear View PUMP1 PUMP2 PUMP3 PUMPS CAUTION PL
40. SERIAL 1 0 12V 12V 12V Ke New Brunswick Scientific User s Guide 158 Figure 56 Gas Mix Controller Schematics GND SERIAL 1 0 SERIAL 1 0 12V THESE ARE THE 9 Li 5 MICRO CONTROLLER ta SS E i L P C B 1273 7000 GND SERIAL 1 0 SERIAL 1 0 12V 12V 12V BioFlo 110 Modular Fermentor 1273 0054 MICRO CONTROLLER P C B 1273 7000 J1 SECTION A SECTION B SECTON A SECTON B 1 5 6 2 3 7 8 9 1 5 6 2 7 9 User s Guide 159 Figure 57 4 Pump Module Schematics 110V 5001 TBOO1 2 oi RED 1 RED Poo co T 39uF 2 BLK 4 N BLU 4 6 WHT WHT 002 TBOO2 mm _ BRN 2 oj RED 4 RED ael 5 BLK ul ES 77 N BLU 4 6 WHT WHT S003 TBOO3 BRN 2 oj RED 7 RED Poo MEM coos 1 77 8 BLK ae N BLU 4 6 WHT WHT 5004 004 m BRN 2 o RED 10 RED Poo MEM 1 M004 39uF 11 BLK yr N BLU 4 6 WHT WHT New Brunswick Scientific User s Guide 160 P001 P002 005 P004 BioFlo 110 Modular Fermentor M1273 0054 Figu
41. Scientific Co Inc reserves the right to change information in this document without notice Updates to information in this document reflect our commitment to continuing product development and improvement Manual Conventions Y amp NOTE Notes contain essential information that deserves special attention N Caution messages appear before procedures which if CAUTION caution is not observed could result in damage to the equipment Warning messages alert you to specific procedures or WARNING practices which if not followed correctly could result in serious personal injury Bold Text in boldface type emphasizes key words or phrases This particular Warning message whether found in the manual or on the unit means HOT SURFACE and therefore represents a potential danger to touch Crush Warning messages alert you to specific Ww procedures or practices regarding heavy objects which if not followed correctly could result in serious personal CRUSH WARNING injury New Brunswick Scientific User s Guide vi P umm gt WARRANTY lt Every Instrument manufactured by the New Brunswick Scientific Co Inc is warranted to be free from defects in material and work manship This apparatus with the exception of glassware lamps and electrodes where supplied is warranted for 1 year against faulty com ponents and assembly and our obligation under this warranty is limited to repairing or replacing t
42. Spargers NBS Part Number Sparger Ring 1 3L M1273 9259 Sparger Ring 3 0L M1273 9256 Sparger Ring 7 5L M1273 9246 Sparger Ring 14 0L M1273 9251 Porous Sparger 1 3L non jacketed vessel M1273 5007 Porous Sparger 1 3L water jacketed vessel M1273 5003 Porous Sparger 3 0L jacketed or non jacketed vessel M1273 5004 Porous Sparger 7 5L jacketed or non jacketed vessel M1273 5005 Porous Sparger 14 0L jacketed or non jacketed vessel M1273 5006 Replacement Vessels Part Description NBS Part Number 1 3L Glass Vessel non jacketed M1273 9907 1 3L Glass Vessel water jacketed M1273 9908 3 0L Glass Vessel non jacketed M1273 9909 3 0L Glass Vessel water jacketed M1273 9915 7 5L Glass Vessel non jacketed M1273 9916 7 5L Glass Vessel water jacketed M1273 9917 14 0L Glass Vessel non jacketed M1273 9918 14 0L Glass Vessel water jacketed M1273 9919 Miscellaneous Parts amp Optional Accessories BioFlo 110 Modular Fermentor 1273 0054 Description NBS Part Number Addition Harvest Bottle Kit 250 mL M1273 9989 Addition Harvest Bottle Kit 500 mL M1273 9990 Air Filter Regulator Kit single manifold for one vessel M1230 3030 Air Filter Regulator Kit four manifolds for up to four vessels M1230 5002 Bearing Housing Cap disposable for autoclaving pack of 10 M1273 9936 O Ring Kit washers amp O rings including headplate ALL vessels M1273 9
43. User s Guide 79 If after autoclaving most of the liquid has left the vessel the autoclave is exhausting too quickly Adjust the autoclave to exhaust more slowly 6 4 Reinstalling the System 1 Position the vessel back on the benchtop If this is a water jacketed vessel place the vessel on the jacket water heater carefully align the water jacket inlet and outlet with the heater plate s rear notch then place the vessel on the heater so the vessel s rubber feet fit into the holes provided for them 2 Connect the heater to the heater connector on the front of the Power Controller 3 Reconnect the water lines to the cooling coil non jacketed vessel or the water jacket inlet and to the exhaust condenser if present A WARNING Cold water and hot glass is a potentially dangerous mix Be sure to let the vessel cool for a few minutes before reconnecting the water line 4 If present remove the pH probe shorting cap and reconnect the pH cable to the probe and the dO2 pH Controller 5 If present remove the 402 probe shorting cap and reconnect dO2 cable to the probe and the dO2 pH Controller 6 Remove the bearing housing cap inspect for wear and replace for next use if necessary and reinstall the motor 7 Reconnect the temperature probe and any other probe sensor cables 8 Remove addition foam trap and or harvest bottle assemblies from the headplate and reinstall them on the base Be sure to finger t
44. V ESSBL VR REN PEE 77 6 31 Sterilization Time and Temperature eese eese neret eerte ene tren entren trennen enne 78 6 4 REINSTATLING THE e eene Ur E WEE D YER PUE 79 6 5 CONFIRMING PH CALIBRATION ccccsesscecececsesseececececsensaececececsensasececececeeseaaeaeceeecsesesuaaececcesesenseaeeeeeeeenes 80 6 6 POLARIZING amp CALIBRATING THE DO2 80 6 7 POWERING THE JACKET WATER 2021202 2000 0 0101000000000000000000000000000 82 7 84 7 1 PRIMARY CONTROL UNIT DISPLAY amp 5 0 0000000000000000000000000002000000000000 84 BioFlo 110 Modular Fermentor M1273 0054 User s Guide xi 9 10 11 7 2 SELECTING THE APPLICATION Ee d eve Neb ese a a ave unt ave 86 7 3 DEEP 87 TA SET THE TEMPERA TURE teet a eee vete tee eee eve ae sa veu vue boe ORNA A 89 7 4 1 eI DA I MAS D ah ee 89 FAD deIpolhtisexs nau Saec pee Dav dedere ee Dag uie ree caue deris m 91 743 Graphie Display sse e P er a ei pe Ie Re d ep ee potete preset ibo 92 7 5 SETTING THE AGITATION LOOP ccccccccecececececececececscecececececececececececececseecec
45. be sure that there is no interference between the probes and the baffle assembly impeller blades or cooling coil 1 Wear protective gloves to protect yourself in case of accidental breakage 2 Lightly coat the dO2 probe with glycerol CAUTION Do not install the dO2 port adaptor in the headplate before inserting the probe Follow the steps below to fit the dO2 port adapter onto the probe first then insert the probe and adapter into the headplate Figure 16 dO2 Probe with Port Adapter exploded CAP 402 PROBE ADAPTER top portion TOP FERRULE BOTTOM FERRULE CN 402 PROBE ADAPTER bottom portion yc TEFLON O RING white PORT O RING black New Brunswick Scientific User s Guide 28 With reference to Figure 16 above 3 Gently slide the top portion of the knurled port adapter part of the probe kit onto the probe 4 Slide the two white ferrules onto the probe the narrower one on top of the deeper cup shaped one 5 Gently slide the bottom portion of the port adapter onto the probe taking care to orient the longer threaded section toward the top of the probe 6 Remove the two O rings installed in the dO2 port first slide the white Teflon O ring onto the probe then follow with the black 12mm port adapter O ring 7 Do not yet close up all the elements of the port adapter 8 Gently insert the probe into the appropriate port see Figures 11 12 amp 13 for reference allowing the O r
46. be used New Brunswick Scientific User s Guide 4 7 NOTE It is good practice to lightly lubricate the underside of the headplate with silicone before installing it on the vessel 4 5 21 Install 1 3L 3 0L or 7 5L Fermentation Vessel Baffle 1 Gently place the baffle tab facing up around all of the other instruments protruding from the headplate including the cooling coil 2 Position the tab between the two uprights of the cooling coil Hold the baffle in place with two fingers when you lift the headplate assembly 4 5 22 Install Headplate 1 Orient the cooling coil uprights toward the back opposite the gradations marked on the vessel glass If this is a 1 3L 3 0L or 7 5L fermentation vessel squeeze and hold the baffle in place opening toward the back with thumb and forefinger You may find it convenient to squeeze the tab with your thumb 2 Carefully lower the headplate easing all of its attachments into the vessel without hitting the glass or the baffle inside if this is a 14 0L fermentation vessel 9 NOTE If you are using a baffle after installing the headplate insert any convenient length of wood plastic or stainless steel do not use any other kind of metal through an unused port to push the baffle down as far as it will go The baffle is stainless steel repeated installations may cause it to retain a compressed position Expand it before you squeeze it for installation so it will spring back a
47. brass body has two hose barbs identified as 1 and 2 by numbers stamped on the body just above the barb Hose barb 1 is the water inlet Hose barb 2 is the water outlet The valve body swivels to facilitate access 8 Connect silicone tubing to the top outlet port of the exhaust condenser Allow enough tubing to run to the water drain Secure the tubing with a plastic tie Figure 17 Water Solenoid Valve MOUNTING VALVE SPLITTER PLUG connect BRACKET X to rear panel of lt 2 Power Controller mA 2 see Figure 2 HOSE BARB 1 S HOSE BARB 2 connect to water connect to cooling source downstream coil or water jacket of pressure regulator inlet VALVE BODY Figure 18a Exhaust Condenser 1 3L 3 0L amp 7 5L Vessels STERILE FILTER WATER OUTLET WATER INLET SET SCREW A P in port adapter EXHAUST PORT HEADPLATE New Brunswick Scientific User s Guide 30 7 NOTE If the weight of the exhaust filter kinks the tubing fasten a short length of stiffening material to the tubing using rubber bands or tie wraps to support the filter Figure 18b Exhaust Condenser 14 0L Vessel Only STERILE FILTER a e water our TTT SET SCREW in port adapter EXHAUST PORT O rim Q gt VATER QUT TO SOLENOID VALVE WATER IN _ id j COOLING CO
48. holder 3 Open the thumb clamp 4 Pull back on the syringe plunger to fill the bottle 5 Close the thumb clamp Close the plunger 6 Observe aseptic techniques to remove the filled bottle and cap it 7 Immediately install a new sample bottle in the holder 7 16 Automatic Foam Control If you have installed a foam probe and set its control mode to Antifoam the addition of antifoam is automatic When the probe detects the presence of foam its signal prompts the Level Controller to signal the Power Controller The Power Controller activates the Antifoam pump to add chemical defoamer from the addition bottle through an addition port When the probe no longer detects foam the pump will be turned off BioFlo 110 Modular Fermentor M1273 0054 User s Guide 113 7 17 Harvesting AN WARNING NEVER PRESSURIZE A GLASS VESSEL Never intentionally block the exhaust to raise vessel pressure Never exceed the maximum pressure specified in this manual This maximum pressure is necessary only to obtain the highest gas flow rates Itis capable of breaking the vessel if the vessel is not properly vented At the end of a batch to harvest the entire culture 1 Aseptically prepare an autoclaved harvest bottle large enough to hold the entire contents of the vessel The bottle cap should be equipped with a sterile filter on one penetration There should also be sufficient silicone tubing on the other penetration to reach from t
49. input BioFlo 110 Modular Fermentor M1273 0054 User s Guide 85 Figure 44 PCU Touchpad Operating Light Numeric Selector keys keys There are also four function keys on the touchpad This key allows you to toggle the display from Main screen to Main screen if you have more than one fermentor on the system It also allows you to return to the Main screen from any other screen This key allows you to retrieve the Language screen or the Systems Detail screen This key allows you to go back to the previous screen It also allows you to cancel any numerical input e g setpoint etc that has not yet been entered This key allows you to enter any touchpad input that you wish to save New Brunswick Scientific User s Guide 86 7 2 Selecting the Application The default Application selection is Fermentation Use selection on the initial setup screen will allow you to verify and or change the application selected for each vessel identified as Unit 1 Unit 2 etc 1 Press the Use selector button English NBS BioFlo 110 Francais o Version 0001 01 X Config 1 10 Deutsch Date 2 2000 Espa ol ER http www nbsc com Use lt More 4 lt 2 The Application screen appears To change the application for any vessel press the Use button next to that unit For this example we will change Unit 2 from Fermentation to Cell Culture Press Here
50. the Enter key to save the new setpoint The Temperature Loop Details screen returns showing the new setpoint on the graphic display s X axis Temp 28 7 C Control Auto Control i Output 100 0 7 z Setpoint o Disp Hi Disp Lo 1 0 7 4 3 Graphic Display To change the range of the Y axis on the graphic display 1 Press the Disp Hi selector button A highlighted edit box appears 2 Using the touchpad enter the desired High value 3 Press the Enter key to save the new value 4 Press the Disp Lo selector button A highlighted edit box appears 5 Using the touchpad enter the desired Low value 6 Press the Enter key to save the new value Press the button to return to the Main Screen BioFlo 110 Modular Fermentor M1273 0054 User s Guide 93 7 5 Setting the Agitation Loop From the Main Screen of the appropriate fermentor press the selector button in the Agit row The Agitation Loop Details screen appears Agit 155 MEME s80 5 H Setpoint 5 Disp Hi Disp Lo O Set the Control Mode the Setpoint and the Display High and Low range in the same way as you did for the Temperature loop N CAUTION Never exceed 200 rpm unless at least one impeller is immersed in liquid 7 6 Setting the pH Loop From the Main Screen of the appropriate fermentor press the selector button in the pH row The pH Loop Details screen opens New Bruns
51. the Set Zero selector button A highlighted edit box opens Calibrate pH 6 52 Zero Std 7 00 QE Set Span Reset Raw Input 5500 Select Reset at any time in the Calibration process to return to the factory set default Zero Standard and Span settings 9 Using the touchpad key in 7 0 the pH of the buffer 10 Reconfirm that the Raw Input reading is stable then press the Enter key to save the value or press X to cancel 11 Press the Set Span selector button As above a highlighted edit box will open in the Set Span row 12 Rinse the electrode with distilled water then immerse it in a second pH buffer solution which is several pH units below pH 7 00 e g 4 00 and allow a few minutes for the system to equilibrate as indicated by a nearly stable Raw Input New Brunswick Scientific User s Guide 68 13 Using the touchpad enter the new Span value which is the buffer pH 14 Reconfirm that the Raw Input is stable then press the Enter key to save the value or press X to cancel 15 To ensure accuracy repeat the above steps using the same two buffer solutions 16 After the values have been set press the gt button to return to the Main Screen CAUTION Do not use hydrochloric acid with the BioFlo 110 for pH control or any other purpose because HCI corrodes stainless steel Over time will damage the stainless steel components Phosphoric and sulfuric 10 m
52. the dO2 Details screen press the Setup selector button The Setup screen opens J dO2 73 1 A Defaults Cascade To None Cascade Agit Hi Limit 1200 Agit Hi Agit Lo Limit 250 Agit Lo 1 Advanced Control P Gain 0 20 P Gain I Gain 1 00 Gain 7 NOTE Selecting Defaults at any time during setup or after settings have been changed will return all settings EXCEPT Cascade To to the default setting Cascades remain in force until you select otherwise 2 Press the Cascade selector button The Cascade screen opens dO2 73 1 Cascade To None Agit Agit Hi Limit 1200 None Agit Lo Limit 250 Advanced Control P Gain 0 20 1 00 Press the Agit selector button The Setup screen returns showing the new cascade parameter New Brunswick Scientific User s Guide 100 7 8 2 2 73 1 Defaults Cascade To Agit Cascade Agit Hi Limit 1200 Agit Hi Agit Lo Limit 250 Agit Lo Advanced Control P Gain 0 20 P Gain 1 00 Gain Press X to return to 402 Details screen or to return to the Main Screen Cascades for Fermentation with Gas Mix Controller When you are using the Gas Mix Controller you have four choices to cascade to for Fermentation Agitation Oxygen Agitation Oxygen or None If you have the optional TMFC module these screens are different See Section 14 for details Cascading to Agitation controls dissolved oxyg
53. the sampler tube and harvest tube are welded into the same tri port to Save space 2 Finger tighten the knurled adapter on the sampler tube then use the Allen key provided to tighten the set screw 4 5 10 Install Thermowell 1 Working from above the headplate insert the thermowell tube into the RTD port see Figures 11 12 amp 13 for reference AN CAUTION Make sure that the thermowell does not touch the cooling coil 2 Finger tighten the knurled adapter on the thermowell 4 5 11 Install Foam Probe If you are using a foam sensor with a foam trap kit 1 Working from above the headplate insert the foam sensor into the appropriate port see Figures 11 12 amp 13 for reference 2 Finger tighten the knurled adapter BioFlo 110 Modular Fermentor M1273 0054 User s Guide 25 4 5 12 Install Foam Exhaust Tube If you are using a foam trap install the foam exhaust tube 1 Working from beneath the headplate insert the foam exhaust tube into the appropriate port close to a headplate clamping nut see Figures 11 12 amp 13 for reference where you will later mount the foam trap 2 Finger tighten the knurled adapter If you need to raise or lower the tube at any time use the Allen key provided to adjust the adapter s set screw 4 5 13 Install Level Probe s If you are using a level probe as part of the antifoam system and or a level probe to detect media level one at a time 1 Working from abov
54. your preferred language Section 5 Before First Use and Section 7 Operation explain the Application Use settings and the choices offered by the More selection They also explain how to use all other screens and available settings New Brunswick Scientific User s Guide 66 5 Press the gt button to open the Main Screen see sample screen below vede Loop alue etpoint py Tr Name Value Setpoint Control Temp 234 30 0 C Auto Agit 134 350rpm Auto Pump A 0 0 0 0 Manual Pump B 0 0 0 0 Manual Pump C 0 0 0 0 Manual pH 6 68 7 00pH Auto dO2 77 1 0 0 Auto Loop Control 10000000 The above screen is only one sample The loop names will reflect the specific modules in your system Press the selector button in the pH row The pH Loop Details screen opens PH 6 68 Control Auto Output 100 0 Control Setpoint Disp Hi Disp Lo Calibrate Setup QOO OC 6 In the pH Loop Details screen press the Calibrate selector button The Calibrate pH screen opens BioFlo 110 Modular Fermentor M1273 0054 User s Guide 67 i Calibrate pH 6 52 Zero Std 7 00 Set Zero Set Span Raw Input 5500 Reset 2 0 7 Rinse the pH electrode with distilled water then immerse it in a pH 7 00 buffer solution Allow a few minutes for the system to equilibrate as indicated by a nearly stable Raw Input reading 8 Press
55. 10 parameters are represented by Register assignments see Register Map in Section 13 3 1 below e The BioFlo 110 implements Modbus functions 03 06 and 16 decimal e All registers are transmitted high byte first then low byte standard Modbus format e All floating point values are stored in two Register pairs with the higher half in the first even Register and the lower half in the second odd Register Therefore floating point values are transmitted as four bytes starting with the highest and ending with the lowest New Brunswick Scientific User s Guide 142 e Character strings are stored in registers with one character per register The high byte of the returned Register value will contain 0 and the low byte will contain the character code e Integer values are stored in a single Register CONTROL TYPE VALUES Control Type Value OFF 0X00 Auto 0X01 Manual 0X02 ON OFF 0X03 P DB 0X04 Total 0 P DB means Proportional with Deadband 13 3 1 Register Map 7 NOTE Programmers take note that all of the following are decimal addresses Loop Number 0 1 2 32 31 Parameters Decimal Address 1 Curr_val 0 2 4 mm 62 0 N Loop 2 2 Setpoint 64 66 68 m 126 64 N Loop 2 3 Control out 128 130 132 ids 190 128 N Loop 2 4 Integral 192 194 196 254 192 N Loop 2 5 Derivative 256 258 260 318 256 N Loop 2
56. 4 RS 422 Receive Data 12 RS 422 Receive Data BioFlo 110 Modular Fermentor 1273 0054 User s Guide 71 5 3 2 Select Communications Protocol If a computer will be used for automatic data logging or process supervision select an appropriate digital communications protocol either ModBus or if you are using NBS BioCommand supervisory software AFS 1 Press the More selector button from the Application Language screen or press the OK key once from the Main Screen or twice from the opening Language Selection Screen to open the System Details screen System Details Unit Base Address Unit 1 ID Unit 2 ID BaseAdd Unit 3 ID Unit 4 ID 3 Comm Protocol AFS Recorder Status Off Comm Recorder 2 Press the selector button next to Comm The Communication Settings screen will open Comm Settings Comm Protocol AFS Mod Bus Baud Rate 9600 AFS Data Bits 8 Parity Even Stop Bits 2 Flow Control None 3 Press the selector button for your choice AFS or ModBus There is no need to press the Enter key Use the X key to return to the Systems Detail screen or the gt key to return to the Main Screen New Brunswick Scientific User s Guide 72 5 3 3 Set Base Addresses You must set base addresses or ID numbers for AFS or your other supervisory software so the software recognizes each fermentor group as a distinct unit 1 Press 1 amp
57. 80 3750 Power Controller Fuse 250V 3 15 Amps P0380 3751 PCU Fuse Slo Blo 250V 2 Amps P0380 3533 Battery Alkaline 1 5 VDC P0480 9030 Exhaust Condenser NBS Part Number Exhaust Condenser 1 3L 3 0L amp 7 5L M1273 9945 Exhaust Condenser 14 0L M1273 9957 Foam Trap Kits NBS Part Number Foam Trap Kit 500 mL bottle all vessels M1273 9943 Foam Trap Kit 250 mL bottle all vessels M1273 9942 Headplate Port Fittings NBS Part Number 6 35mm port to 4 76mm tube Adapter compression M1273 5042 12mm port to 4 76mm tube Adapter compression M1273 5055 6 35mm port to 6 35mm tube Adapter set screw M1273 5054 12mm port to 6 35mm tube Adapter set screw M1273 5056 12mm Probe Adapter compression 1273 5040 12mm to 12mm Adapter set screw M1273 5058 6 35mm Plug M1273 9405 12mm Plug M1273 9406 19mm Plug M1273 9407 Heaters amp Heat Blankets NBS Part Number Jacket Water Heater 1 3L amp 3 0L vessels M1273 3107 Jacket Water Heater 7 5L amp 14L vessels M1273 3108 Heat Blanket 1 3L vessel M1273 9931 Heat Blanket 3 0L vessel M1273 9932 Heat Blanket 7 5L vessel M1273 9930 Heat Blanket 14 0L vessel M1230 9933 Impellers NBS Part Number 6 bladed Rushton type 52 mm 1 3L amp 3 0L M1273 9291 6 bladed Rushton type 59 mm 7 5L M1273 9292 6 bladed Rushton type 74 mm 14 0L M1273 9293 Pitched Blade
58. 900 Septum Kit for 12mm port M1273 3031 Spare Parts Kit 1 3L 3 0L non jacketed vessel M1273 9991 Spare Parts Kit 7 5L 14 0L non jacketed vessel M1273 9992 Spare Parts Kit 1 3L 3 0L water jacketed vessel M1273 9998 Spare Parts Kit 7 5L 14 0L water jacketed vessel M1273 9999 Water Regulator Filter Kit single manifold for one vessel M1117 2040 Water Regulator Filter Kit four manifolds for up to four vessels M1273 5001 User s Guide 123 Module Kits Power Controller NBS Part Number Kit contains the power controller 4 cable guides power cord 100 120V 200 240V M1273 3100 M1273 3110 Primary Control Unit PCU NBS Part Number Kit contains PCU display amp keypad floppy disk drive battery back up 2 cable guides power line M1273 3101 dO2 pH Controller NBS Part Number Kit contains the dO2 pH controller 1 Ingold gel pH probe amp 1 2 headplate probe adapters 2 probe cables 2 probe shorting ngold polarographic dO2 probe caps module entry cable For 1 3L Vessel M1273 9850 For 3 0L Vessel M1273 9851 For 7 5L Vessel M1273 9852 For 14 0L Vessel M1273 9853 dO2 pH Controller only M1273 3102 Level Controller NBS Part Number Kit contains the level controller 1 level sensor 1 probe cable cable guides module entry cable 1 headplate probe adapter 2 For 1 3L Vessel M1273 9841 For 3 0L Vessel
59. 9900678 DOULEUR New Brunswick Scientific User s Guide 84 7 OPERATION 79 NOTE Before operating a water jacketed vessel make sure it is properly installed on the vessel jacket water heater and that the heater is properly connected see Section 6 4 before you set the desired working temperature 7 1 Primary Control Unit Display amp Controls The Primary Control Unit PCU serves as the operator interface A single PCU can control up to four sets of control modules but no more than sixteen total for operating four vessels simultaneously If you plan to run more than four vessels another PCU must be added for each group of four The PCU has universal AC power input that can be set to accommodate electrical services between 100 and 240 Volts It can be plugged into the outlet on the back of the Power Controller see Figure 2 or plugged directly into your electrical service The PCU is equipped with battery back up to safeguard memory in case of power loss Figure 43 PCU Front Panel Display Screen Operating Light Touchpad 2222 Lo The user interface touchpad has 12 numeric keys As you can see in the drawing below these keys are the numbers 0 through 9 a decimal point and a sign There are also 8 selector keys which allow the user to choose specific loop detail screens from the main screen and to select specific interactive screens for operational
60. Application Unit 1 Fermentation Use Unit 2 Fermentation Use Unit 3 Fermentation Use 4 Unit4 Fermentation Use lt lt 1 Back 3 Press Unit 2 s Use selector button The display changes to Application selection screen see sample screen below Note that the alignment of choices Fermentation or Cell Culture on this screen does not relate to the units that appear on the left You have already selected Unit 2 therefore your choice in this new screen will only affect Unit 2 BioFlo 110 Modular Fermentor M1273 0054 User s Guide 87 Application Unit 1 Fermentation Unit 2 Fermentation Fermentation Unit 3 Fermentation Cell Culture Unit 4 Fermentation 4 Press the Cell Culture selector button The display automatically returns to the previous screen showing Unit 2 s application as Cell Culture Application EM Unit 1 Fermentation Use lt Unit 2 Cell Culture Use lt 7 Unit 3 Fermentation Use lt Unit 4 Fermentation Use lt Back lt Follow the same steps time you wish to verify or change application for vessel Unit 7 3 Main Screen 1 Press the 1 button to open the Main Screen see sample screen below New Brunswick Scientific User s Guide 88 Current Loop Loop Loop Name Value Setpoint Control Mode Name Value Setpoint Control Temp 23 4 30 0 C Auto Agit 134 350rpm Auto lt
61. D silicone tubing NBS P0740 2505 Connect the outlet of the rotameter to a sterile filter taking care to observe the flow direction requirements if stamped on the filter Connect the filter outlet to the sparger inlet With the Gas Mix Controller Familiarize yourself with the front panel of the controller and its features GAS INLETS Four push in inlets for delivery of Oxygen Carbon Dioxide Air and Nitrogen from your source GAS STREAM One push in outlet for sequential output of various individual OUTLET gases as controlled by the Gas Mix Controller and for delivery to the rotameter or Thermal Mass Flow Controller inlet The rotameter or TMFC outlet delivers air gases to the sparger via the vessel headplate Figure 39 Gas Mix Controller Front Panel CARBON DIOXIDE INLET p CABLE GAS MIX GUIDE BIOFLO 110 9 OG AIR INLET OXYGEN INLET GAS STREAM NITROGEN INLET OUTLET V 2 8 ve Attach pressure regulated clean air and other gases to the push in inlets of the 4 Gas controller using the blue polyurethane tubing supplied NBS P0470 3111 0 125 inch ID 0 250 inch OD Connect the gas stream outlet port of the 4 Gas controller to the inlet of the rotameter Connect the outlet of the rotameter to a sterile filter taking care to observe the flow direction requirements if stamped on the filter Connect the filter outlet to the sparger inlet Install Cable Gu
62. DLE RTD THERMOWELL TRI PORT PLUG 19mm SPARE PLUG 12mm SPARE Each bolt is a possible mounting position for a bottle holder 4 5 2 Install Heat Blanket 1 Wrap the heat blanket as snugly as possible around the vessel taking care to leave one of the viewing windows facing forward You will probably want to orient the blanket so the power cord connection is out of the way 2 Secure the blanket by overlapping the Velcro strips and pressing them together A WARNING NEVER cut any portion of the heat blanket NEVER fold the heat blanket or place any weight upon it For storage always lay the heat blanket flat BioFlo 110 Modular Fermentor M1273 0054 User s Guide 21 4 5 3 Install Vessel in Vessel Stand 1 Place the clamping ring on the vessel stand align the clamping ring holes with the vessel stand pillars then slide it into place It will come solidly to rest on the shoulder of each pillar 2 Place sections of U shaped rubber bumper equidistantly around the inside of the clamping ring there are three pieces for 1 3L amp 3 0L vessels and two larger pieces for 7 5L and 14 0L vessels Press each section securely against the inner edge of the ring Figure 14 Upper Vessel Bumper Installation Vessel Clamping Section of Ring rubber BUMPER NOTE your vessel may only have two 3 Gently lower the glass vessel through the center of the clamping ring until the vessel flange rests snugly agai
63. Fermentor 1273 0054 User s Guide 55 Figure 33 Motor Installation MOTOR SHORT SLOT SHOULDER STUDS ON BEARING HOUSING MOTOR COUPLING 2S NOTE Make sure the O ring is lubricated PINS ON BEARING SHAFT BEARING HOUSING A WARNING Never remove or install the motor while it is running ZA NOTE Periodically check the condition of the plastic cushioning P0740 3111 as you remove or reinstall the motor Replace it when it begins to wear 4 8 Install the Rotameter CAUTION Verify that the source of air is regulated at 10 PSI MAXIMUM 1 Hang the rotameter on the left or right side of the Power Controller or PCU whichever is most convenient Using a Phillips head screwdriver mount the meter bracket using the screws provided 2 Use silicone tubing to connect the air source to the bottom air inlet hose barb on the rotameter see Figure 34 New Brunswick Scientific User s Guide 56 Figure 34 Rotameter Side View AIR OUTLET BARB Connect to sterile inlet filter on vessel headplate __ FRONT PANEL GAUGE HANGING BRACKET e AIRFLOW y ADJUSTMENT AIR INLET BARB ong KNOB Connect to clean air source 3 Attach another piece of silicone tubing to the top air outlet hose barb see Figure 54 above Insert the other end of this tubing into the inlet sterile filter on the top of the vessel headplate 4 Verify that the exhaust port is conne
64. Guide to Operations BioFlo 110 Modular Benchtop Fermentor MANUAL NO M1273 0054 Revision H August 8 2007 NEW BRUNSWICK SCIENTIFIC CO INC BOX 4005 44 TALMADGE ROAD EDISON NJ 08818 4005 Telephone 1 732 287 1200 e 1 800 631 5417 Fax 732 287 4222 e Telex 4753012 NBSCO Internet http www nbsc com e E mail nbsc com BioFlo 110 Modular Fermentor 1273 0054 User s Guide INTERNATIONAL OFFICES BELGIUM New Brunswick Scientific NV SA Stationsstraat 180 4 3110 Rotselaar Belgi Belgique Tel 32 0 16 56 28 31 Fax 32 0 16 57 27 53 E mail sales nbsnv sa be CHINA New Brunswick Scientific Co Inc A903 904 Yin Hai Building No 250 Cao Xi Road Shanghai 200235 P R China Tel 86 21 6484 5955 or 5966 Fax 86 21 6484 5933 E mail nbschc online sh cn FRANCE New Brunswick Scientific SARL 12 14 Rond Point des Champs Elys es 75008 Paris France Tel 33 0 1 5353 1511 Fax 33 0 1 5353 1557 E mail sales nbssarl fr New Brunswick Scientific GERMANY New Brunswick Scientific GmbH In Der Au 14 D 72622 N rtingen Deutschland Tel 49 0 7022 932490 Fax 49 0 7022 32486 E mail sales nbsgmbh de THE NETHERLANDS New Brunswick Scientific BV Kerkenbos 1101 6546 BC Nijmegen P O Box 6826 6503 GH Nijmegen Nederland Tel 31 0 24 3717 600 Fax 31 0 24 3717 640 E mail sales nbsbv nl UNITED KINGDOM New Brunswick Scientific UK Ltd 17 Alban Park St
65. H units Off Automatic loops total PID adjustable deadband liquid additions 4 gas blending dO2 with dO2 pH Module 0 100 Off Automatic PID agitation cascade sparge on demand 4 gas blending Pump 1 0 100 00 Off Manual Automatic with conduction probes Pump 2 0 100 00 Off Manual Automatic with conduction probes Pump 3 0 100 00 Off Manual Automatic with conduction probes Pump 4 0 100 00 Off Manual Automatic with conduction probes 2 with Gas Mix 0 100 Off Manual Automatic Controller Touchpad User interface 24 touch sensitive buttons Internal A drive Supports flash e prom upgrades Computer connectivity Serial port supports AFS and ModBus protocols Recorder connectivity Four 0 2 5 volt outputs Power 85 265 VAC 50 60 Hz 100 VA Power Switch Located on back Memory Back Up Two AA alkaline batteries Fusing 2 Amp 250V Slo Blo Power Indicator Light Located on front Other e Dual Rotameter hangers Cabling tubing organizers BioFlo 110 Modular Fermentor 1273 0054 User s Guide 125 BioFlo 110 Power Controller Weight 16 Ib 7 2 kg Dimensions Height 8 9 16 in 21 8 cm Depth 12 in 30 5 cm Width 12 in 30 5 cm allow inches 7 6 cm more front and rear for cable access Agitation Drive e Lift off electric motor Self
66. ID silicone only 50 Hz 12 RPM 60 Hz 14 4 RPM 1 50 inch 0 5 mm 0 25 ml min 0 30 ml min 1 32 inch 0 8 mm 0 59 ml min 0 71 ml min 1 16 inch 1 6 mm 2 62 ml min 3 14 ml min 1 8 inch 3 2 mm 9 60 ml min 11 5 ml min 3 16 inch 4 8 mm 19 6 ml min 23 5 ml min BioFlo 110 Modular Fermentor 1273 0054 User s Guide 127 11 3 Tubing The following tubing sizes are provided with your BioFlo 110 system Description ID OD Length NBS Part Number Blue polyurethane 1 8 inch inch 50 feet P0740 3111 3 2 mm 6 35 mm White silicone 3 16 inch 5 16 inch 25 feet P0740 2505 4 8 mm 7 9 mm White silicone 1 16 inch 3 16 inch 50 feet P0740 2396 1 6 mm 4 8 mm White braided PVC inch 0 438 inch 15 feet P0740 1631 6 35 mm 11 13 mm 11 4 Vessels BioFlo 110 Non Jacketed Vessels Vessel Volume Total Volume 1 3L 3 0L 7 5L 14 0L Working Max 1 0L 2 2L 5 6L 10 5L Volume Min 0 4L 0 8L 1 5L 4 01 Vessel Weight with Headplate 15 Ib 20 5 Ib 39 5 Ib 43 Ib Stand Drive 6 8 kg 9 3 kg 18 kg 19 5 kg Inserts amp Plugs Stand Non Jacketed Stainless steel four legs for stability high friction feet to Vessels eliminate walking Aeration Max Flow Rate Fermentation 2 VVM Cell Culture 0 5 VVM Sparger Stainless steel ring sparger with removable EPDM plug Maximum Sparge 10 PSIG Pressure Filters 0 2u absolute filters resterilizable Exhaust Con
67. IL 4 5 18 Install Sampler The optional BioFlo 110 sampler system is designed to aseptically remove batch samples from the vessel The entire installation is easily autoclaved in place on the vessel If you are using the sampler install the kit as follows using Figures 19a amp 19b below for reference 1 Remove a headplate clamping nut adjacent to the location of the sampler tube 2 Mount the metal sampler bottle holder arm on the clamping screw and secure it in place with the clamping nut BioFlo 110 Modular Fermentor M1273 0054 User s Guide 31 Figure 19a Sampler Harvest System 1 3L Vessel THUMB CLAMP 1 8 SILICONE TUBING REDUCING SYRINGE FILTER ELBOW to 3 16 tubing on sampler holder 12 S SAMPLER HARVEST 4 l u PORT SYRINGE SAMPLER BOTTLE ea HEADPLATE HOLDER SAMPLER BOTTLE SPARE HEADPLATE CLAMPING NUT Tpit LLLLI HEADPLATE CLAMPING SCREW SAMPLE TUBE i HARVEST TUBE Figure 19b Sampler System 3 0L 7 5L amp 14 0L Vessels 3 16 SILICONE TUBING FS DW SAMPLER PORT UE zr i 1 HEADPLATE THUMB CLAMP m I N HH 9 SYRINGE A SYRINGE FILTER SAMPLER BOTTLE HOLDER
68. IONS T amp NOTE Only a qualified technician should perform the procedures contained in this chapter A WARNING Always disconnect the power cord from the Power Controller and the PCU before undertaking any of the following procedures 12 1 Replacing Power Controller Fuses There are eight replaceable fuses inside the power controller Fuse Number Item Controlled Fuse Number Item Controlled F1 Pump A F5 Acid Pump F2 Pump B F6 Water Solenoid Valve F3 Pump C F7 Heater F4 Base Pump F8 Heater To replace one of these fuses Unplug all power cords from the system Disconnect the Power Controller from any other modules Unplug the water solenoid valve from the rear panel Unplug the motor RTD and heater cables from the front panel Loosen the two screws that secure the water solenoid valve assembly in place then remove the assembly by sliding the mounting plate up until the slotted holes allow you to lift the assembly away from the screws 6 Unscrew the ten side mounting screws that hold the Power Controller cover in place Retain them for reuse 7 Carefully pull the cover straight up until it separates from the base There will be some resistance until it comes unclipped Be careful not to pull on the ground wire that is attached to the front of the left side from the rear 8 Rest the cover on its front panel adjacent to the base New Brunswick Scientific User s Guide 132
69. N 2 SPARGER STAND COOLING COIL Familiarize yourself with the arrangement of the headplate ports as shown in the following diagrams before proceeding with the vessel assembly You may find it more practical to change the arrangement the variety of ports and adapters will easily accommodate your needs For easy reference see Appendix E for a mix and match summary of port sizes and the types of adapters and inserts available New Brunswick Scientific User s Guide 18 4 5 1 Headplate Figure 11 1 3L Headplate EXHAUST SPARGER COOLING COIL dO2 PROBE HARVEST SAMPLER SPARE ASSEMBLY pH PROBE TRI PORT RTD THERMOWELL Each bolt is a possible mounting position for a bottle holder BioFlo 110 Modular Fermentor M1273 0054 User s Guide 19 Figure 12 3 0L Headplate HARVEST TUBE COOLING COIL EXHAUST SPARGER COOLING COIL SS 4052 Mounting position for Sampler Assembly SAMPLE RTD THERMOWELL LEVEL PROBE dO2 PROBE Each bolt is a possible mounting position for a bottle holder New Brunswick Scientific User s Guide 20 Figure 13 7 5L 14 01 Headplate COOLING COIL SPARGER COOLING COIL pH PROBE HARVEST SAMPLER TUBE EXHAUST MOUNTING POSITION FOR SAMPLER ASSEMBLY LIFTING HANDLE 402 PROBE PLUG 6 35mm SPARE LIFTING HAN
70. NOTE Figure 7 below is an illustration of a sample installation of two vessels with their control modules For easy access to the touchpad and display screen and for your working comfort we suggest that you place the PCU at the top of the stack closest to the operator The illustration below is more schematic than realistic 7 NOTE No more than 16 modules any combination can be supported by one PCU BioFlo 110 Modular Fermentor M1273 0054 User s Guide 11 Figure 7 Sample BioFlo 110 Installation For clarity cables and tubing are not shown in this drawing MOTOR ete b UNIT 1 Ls VESSEL in Vessel amp Stand dua Mul mim sl dm jj re F m B MES UNIT 2 ADDITION CONTROL BOTTLE UNIT 1 MODULES mounted on CONTROL Vessel Base MODULES UNIT 2 VESSEL in Vessel Stand A closer look PCU with display screen and touchpad Gas Mix Module ya ul 000 D Level Control Module dO2 pH Control Module Power Controller with Rotameter 110 Fermentor Bioreactor New Brunswick Scientific User s Guide 12 4 3 Interconnecting the Modules All BioFlo 110 control modules are interconnected to share power and communications A WARNING Make sure the Power Controller and Primary Control Unit PCU are switched off and unplugged from the power source before connecting or disconnecting any equipment XA NOTE
71. P ERREUR 62 4 11 INSTALL CABLE GUIDES MTS 63 4 12 VOLTAGE SELECTION AND FEUSING eere poe E REEF FRE ERR e EHE Ee Fb ve Pete rev reus ded 64 4 13 CALIBRATING THE PH PROBE uiro e he EE PEN NER REESE E NEAR NE Re rev Tee beue 65 5 2200006 vorn se even eo ao Fe eV o co ee pw ae ob Po eV o ule voe eee sone eU vn oV sdevsess ssutessvesveds iseteessdsbeesvecsbestvesecees 69 5 1 SELECT DISPLAY LANGUAGE cccccccccssssssscecececsessnascecececsesseaececececeessnaeaeceesesensaaeaecececeeseaaeaeceeeceensaaees 69 5 2 ASSIGN VESSELE ADDRESS nba WR Mom oma ones 69 53 FOR PC SUPERVISION 70 551 Connect to Supervisory System iere eere tiep re o e Reo aate 70 5 3 2 Select Communications 71 5 3 3 Set Base Addresses esc rex 72 5 3 4 Set Chart Recorder for Data 72 6 STERILIZATION amp 2 75 61 INITIAL PREPARATION FOR AUTOCLAVING 1 4 200000 00000000000000000000 76 GLI Filling the Water Jacket eoe Rae etae de qi aem 76 6 2 ADDITIONAL PREPARATION FOR 4000 2 4410000 0 000000 02080200000 76 6 3 AUTOGLAVING THE
72. UG IN PUMP CABLES ONLY INTO POWER CONTROLLER INLETS oO DO NOT USE INLET MARKED PCU DES POMPES UNIQUEMENT AUX PRISES DESIGNEES PUMP SUR MODULE DE CONTR LE DE PUISSANCE NE JAMAIS UTILISER LA PRISE PCU WARNING VOLTAGE DISCONNECT INCOMING POWER BEFORE SERVICING ATTENTION HAUTE TENSION DEBRANCHER L ALIMENTATION LECTRIQUE NT TOUTE INTERVENTION Number POWER INLETS each pump individually connected to Power Controller New Brunswick Scientific User s Guide 10 4 1 7 Other Optional Modules Currently an optional Thermal Mass Flow Controller is available See Section 14 for all pertinent details installation operation maintenance troubleshooting and specifications 4 2 Stacking Modules The Power Controller the largest and heaviest of the BioFlo 110 modules is intended to be the base unit of any stack You may stack any of the other modules on top of it in any order Every module has indentations on top to accommodate the feet of any module stacked on it Each Power Controller and any of the control modules dO2 pH level gas mix and or 4 pump associated with it should be stacked beside the vessel they support The modules should be within easy reach of the sensor cables and other leads that will plug into the stack If you are running more than one vessel from the PCU you may wish to place the PCU at the top of the stack most convenient to the operator 79
73. acter The Slave shall respond to a Change command from the Master with an ACK 06H character If the Slave detects an error in the transmission from the Master it shall make no response If the Slave successfully receives a transmission from the Master it must begin the appropriate response in less than 200 mSec New Brunswick Scientific User s Guide 140 13 2 2 13 2 3 Protocol All Slaves must respond to the Requests for data shown in Section 13 2 3 below The Response Header for each Request must be as shown in Section 13 2 3 below All Headers must contain 9 characters where necessary spaces are used to complete the 9 characters In Section 13 2 3 a space is represented by Sp The format of the Response must be as shown in Section 13 2 5 below All Messages consist of a Header plus 10 Fields All Fields must contain 6 characters where necessary spaces are used to complete the 6 characters If there is no data for a particular Field the Slave must fill that Field with space 20H characters All Slaves must respond to Change commands as shown in Section 13 2 4 below The Headers for each Change command are shown in Section 13 2 4 below The Slave response to a Change command shall be an ACK 06H character Even if a Change command is not applicable or not implemented by the Slave the Slave must still acknowledge the command When a Field in a Change command is blank the Slave shall not change the se
74. alf Bring the two halves of the headplate clamping ring together under the vessel flange with the mounting plates on the bottom for easy access from below Align the mounting plates with their corresponding holes on the other ring half and drop in the remaining Phillips head screws Tighten the screws to fasten the ring in place Install Vessel on Base Plate Place the base plate on a level surface Lightly lubricate the base plate O ring and seat it securely in its groove Fit the one piece water jacket guard rubber gasket around the outside of the bottom vessel flange against the water jacket see Figure 26 on the following page With the clamping screws in place on the ring fit the bottom clamping ring onto the base plate BioFlo 110 Modular Fermentor M1273 0054 User s Guide 41 Figure 26 Water Jacket Guard Installation top view Bottom Vessel Rubber Gasket Flange M1155 9902 1 3L amp 3 0L M1227 9903 7 5L amp 14 0L 5 With the gradations marked on the glass facing front toward the user slide the vessel into the bottom clamping ring until it rests securely against the base plate Make sure the water inlet tube stands free not kinked inside the water jacket 6 Finger tighten the six knurled thumb screws to securely attach the clamping ring to the base plate This seals the water jacket 4 6 4 Install Baffle 14 0L Fermentation Vessels ONLY For installation of th
75. aligning motor and vessel shafts e Direct drive with surface seal for fermentation and cell culture e Magnetic drive for cell culture Speed Range e Fermentation 50 1200 rpm Cell culture 30 300 rpm Impellers Rushton optional pitched blade Temperature Range 5 C above ambient temperature to 70 C System 20 40 0 1 C 0 5 at other temperatures Accuracy Control Stability 0 1 C Sensor One 100 platinum RTD input located on front of console Control control algorithm SSR PWM For heat blanket and for cooling solenoid valve Outputs Electrical 115 230 VAC 50 60 Hz 1500 VA Output Connectors e Five controlled PWM IEC outlets for pumps e Vessel Heater e Vessel Cooling Solenoid Valve e One switched mains outlet for PCU e Agitation motor Inputs e Tachometer joint with agitation output e RTD temperature probe Power Switch Located on front Pilot Light Located on front Illuminates when power is on Other e Hangers for 2 Rotameters e Hanger for cooling water valve e Cable tubing organizers BioFlo 110 dO2 pH Controller Weight 5 Ib 2 3 kg Dimensions Height 2 5 8 in 6 7 cm Depth 12 in 30 5 cm Width 12 in 30 5 cm allow inches 7 6 cm more front and rear for cable access Inputs pH Probe NBS supplied cable Dissolved Polarographic electrode with NBS supplied cable Oxygen Probe Control Options See PCU Specification Power 12 VDC supplied by PCU New Brunswick Scientific User s
76. ater Solenoid Valve 29 18a Exhaust Condenser 1 3L 3 0L amp 7 5L Vessels 29 18b Exhaust Condenser 14 0L Vessel only 30 19a Sampler Harvest System 1 3L Vessel 31 19b Sampler System 3 0L 7 5L amp 14 0L Vessels 31 20 Foam Trap 33 21 Water Jacketed Vessel Assembly 36 22 1 3L jacketed Headplate 37 23 3 0L jacketed Headplate 38 24 7 5L amp 14 0L jacketed Headplate 39 25 Installing the Headplate Clamping Ring 40 26 Water Jacket Guard Installation top view 41 27 pH Probe with Port Adapter exploded 45 28 dO2 Probe with Port Adapter exploded 46 29 Water Solenoid Valve 48 30a Exhaust Condenser 1 3L 3 0L amp 7 5L Vessels 49 30b Exhaust Condenser 14 0L Vessel only 49 31a Sampler Harvest System 1 3L Vessel 50 31b Sampler System 3 0L 7 5L amp 14 0L Vessels 51 32 Foam Trap 52 33 Motor Installation 55 34 Rotameter Side View 56 35 Power Controller Front Panel 59 36 dO2 pH Controller Front Panel 60 37 Level Controller Front Panel 61 38 4 Pump Module Front View 62 BioFlo 110 Modular Fermentor 1273 0054 User s Guide 163 Figure Caption Page Number 39 Gas Mix Controller Front Panel 63 40 Cable Guide 64 41 Angled Autoclave Rack Option for 7 5L amp 14 0L Vessels 78 42 Jacket Water Heater Bottom View 83 43 PCU Front Panel 84 44 PCU Touchpad 85 45 Fuse Holder Power Inlet PCU 117 46 Voltage Selection 133 47 Fuse Holder Power Inlet
77. ature 78 Sterilization Time 78 T Table of Contents ix Temperature Probe Connection 60 Terminators for Unused Connectors 138 Thermostat Reset Button 82 Thermowell Installation of 24 43 TMFC Cascades for Cell Culture 151 Cascades for Fermentation 149 Cleaning 152 Loading Software for 145 Operating the 146 Setup for Cell Culture 150 Setup for Fermentation 147 Specifications 153 Troubleshooting 153 Troubleshooting 118 153 Tubing Part Numbers 121 Tubing Specifications 127 BioFlo 110 Modular Fermentor M1273 0054 U Unpacking boxes 3 Unused Ports 33 53 Utilities Requirements for 4 V Verification against Packing List 3 Vessel Assemblies Replacement Part Numbers 119 Vessel Assembly Non Jacketed 16 Water Jacketed 35 40 Vessel Bumpers 21 40 Vessel Stand 16 21 Vessels Part Numbers 122 Voltage Selection 133 WARNING Symbol for v Warranty vi Water Filter Regulator Kit Installation of 58 Part Numbers 122 Water Jacket Filling the 76 Wet Off 61 110 111 Wet On 61 110 111 112 User s Guide
78. avoid touching the glass with any metal object Use non abrasive cleaners only and clean with soft brushes no sharp ends or bristles Any surface that comes into contact with any portion of the vessel must be clean and non abrasive Only finger tighten the knurled headplate bolts and port adapters Over tightening puts undesirable pressure on the glass Keep the glass free from contact with any diamond material diamong jewelry industrial diamonds or diamond dust from grinding wheels BioFlo 110 Modular Fermentor 1273 0054 User s Guide 15 Whenever you assemble or disassemble the vessel components if you need to lay the drive assembly aside while it is still attached to the headplate and the agitation impeller shaft note that thereis a correct and an incorrect way to position the assembly on a flat surface The wrong way which is resting the headplate and impeller shaft on a surface see Figure 9a below puts the impeller shaft at risk for damage Figure 9a WRONG Handling of Drive Assembly HEADPLATE DRIVE ASSEMBLY IMPELLER SHAFT The correct way which is resting the drive assembly and headplate on the surface see Figure 9b below protects the impeller shaft from bearing weight Naturally you will have to take care not to hit the shaft as you work around it Figure 9b CORRECT Handling of Drive Assembly HEADPLATE DRIVE ASSEMBLY IMPELLER SHAFT New Brunswick Scientific User s Guide
79. aximum concentration acids are acceptable and are commonly used for pH control BioFlo 110 Modular Fermentor M1273 0054 User s Guide 69 5 BEFORE FIRST USE This section provides critical pre operation instructions The BioFlo PCU display screens are available in English French German and Spanish Before you operate your BioFlo 110 for the first time be sure to select the preferred language for your PCU display screens Be sure also to configure the PCU and all other control modules so they can communicate with each other and if desired with an outside computer 5 1 Select PCU Display Language When the PCU is powered on the display shows the following screen English NBS BioFlo 110 Francais Version 0001 01 Config 1 10 Deutsch Date 2 2000 Espanol http www nbse com NOTE This is a sample Use screen Your specific Version Config amp Date More zx may differ SELECTOR BUTTONS The default language setting is English If you prefer to use another screen language this is your opportunity to change the setting if you have not already done so 1 Press the selector button next to your preferred language 5 2 Assign Vessel Address Assign one address to all control modules associated with a single vessel As the PCU can control as many as four vessels it is essential that each vessel s control module stack have one unique address New Brunswick Scientific User
80. bes must be polarized for several hours after autoclaving and before calibration according to the manufacturer s instructions Polarization can be accomplished by plugging dO2 probe cable into the dO2 pH Controller and leaving it on for several hours Alternatively battery powered polarizers are available for this purpose From the Main Screen of the appropriate fermentor press the selector button in the 402 row The dO2 Loop Details screen opens 602 o Control Auto Control o v ko F Output 55 Setpoint o Disp Hi l Disp Lo 0 Calibrate Setup BioFlo 110 Modular Fermentor M1273 0054 User s Guide 81 The procedures to set the Control Mode the Setpoint and the Graphic Display High and Low parameters are the same as for the Temperature and Agitation Loops 1 After autoclaving wait until the media reaches operating temperature You can speed the cooling process by entering the Temperature setpoint agitating and sparging 2 In the 402 Loop Details screen press the Calibrate selector button The dO2 Loop Calibration screen opens Calibrate 02 0 o 2 0 00 Set Zero Set Span 1 Raw Input 1022 Reset 1 0 3 Press Set Zero selector button A highlighted edit box opens Calibrate 402 0 Zero Std 0 00 Set Zero Set Span 1 Raw Input 1022 Reset 1 0 4 Using the touchpad key 0 into the highligh
81. ces indicated above provide a recommenced starting point As working volumes and agitation rates change you may wish to adjust the impeller location s 79 NOTE It is good practice to lightly lubricate all O rings port threads and adapter threads with silicone grease before you install equipment in the headplate Also inspect the headplate O ring to be sure it is securely seated in its groove 4 6 6 Install Sparger 1 From beneath the headplate insert the sparger tube into the sparger port see Figures 22 23 amp 24 for reference 2 Finger tighten the knurled adapter on the sparger then use the Allen key provided to tighten the set screw Do not overtighten AN CAUTION Finger tighten only any adapter that has a white Teflon ferrule tapered cone shaped insert under the Teflon washer The ferrule can deform under too much pressure BioFlo 110 Modular Fermentor M1273 0054 User s Guide 43 4 6 7 Install Harvest Tube 1 Working from beneath the headplate install the harvest tube in the harvest port see Figures 22 23 amp 24 for reference If you are using the 1 3L vessel the harvest tube and sampler tube are welded into the same tri port to save space When the headplate is in place on the vessel the bottom of the harvest tube should rest at the bottom of the vessel 2 Finger tighten the knurled adapter on the harvest tube then use the Allen key provided to tighten the set screw Do not overtighten
82. cone tubing between the lower cooling water inlet port of the exhaust condenser and the T connector upstream of the water solenoid valve Secure at both ends with a plastic tie 7 Connect the T connector inlet see Figure 30b to hose barb 2 see NOTE and Figure 29 below on the water solenoid valve The valve is on the side of the Power Controller Secure with a plastic tie 79 NOTE The water solenoid valve s brass body has two hose barbs identified as 1 and 2 by numbers stamped on the body just above the barb Hose barb 1 is the water inlet Hose barb 2 is the water outlet The valve body swivels to facilitate access 8 Connect silicone tubing to the top cooling water outlet port of the exhaust condenser Allow enough tubing to run to the water drain Secure the tubing with a plastic tie Figure 29 Water Solenoid Valve MOUNTING VALVE SPLITTER PLUG connect BRACKET to rear panel of HOSE BARB 1 connect to water source downstream of pressure regulator VALVE BODY BioFlo 110 Modular Fermentor M1273 0054 Power Controller see Figure 2 HOSE BARB 2 connect to cooling coil or water jacket inlet User s Guide 49 Figure 30a Exhaust Condenser 1 3L 3 0L amp 7 5L Vessels STERILE FILTER WATER OUTLET SET SCREW WATER INLET ES in port adapter EXHAUST PORT 79 NOTE If the weight of the exhaust filter kinks the tubing fasten a short len
83. cted to a filter If you are using the optional exhaust condenser the filter should be attached to its outlet 5 Read the airflow at the top flat edge of the float To increase airflow turn the adjusting knob counterclockwise To decrease airflow turn it clockwise Rotameter Maximum Flow Rate Application Vessel Size 1L 3 0L 7 5L 14 0L Fermentation 2 5 Lom 5 Lpm 11 4 Lom 20 Lpm Cell Culture 0 5 Lpm 1 2 Lom 2 5 Lom 5 Lpm 7 NOTE Adjust the airlow according to your specific application A commonly suggested airflow for a typical culture is 1 VVM vessel volume per minute or less for fermentation and 0 05 VVM or less for cell culture unnecessarily high airflow can induce foaming 4 9 Install Liquid Addition Systems Addition ports are used to feed base acid nutrients and media into the vessel To install an addition system BioFlo 110 Modular Fermentor 1273 0054 User s Guide 57 4 10 4 10 1 4 10 2 Install a sterile 0 2 u filter on one of the two penetrations on the addition bottle cap Be sure to respect the proper flow direction if stamped on the filter Connect a length of silicone tubing securing it with a plastic tie to the addition tube in the other penetration of the bottle cap Clamp it off on the top After autoclaving thread the tubing through the desired peristaltic feed pump Aseptically connect the tubing securing it with a plastic tie to t
84. de screen opens dO2 73 1 Cascade To None Agit Agit Hi Limit 1200 O2 Agit Lo Limit 250 Agit O2 None Advanced Control P Gain 0 20 l Gain 1 00 3 Press the Agit selector button The Setup screen returns showing the new cascade parameter New Brunswick Scientific User s Guide 102 7 8 3 7 8 4 J dO2 73 1 Defaults Cascade To Agit Cascade Agit Hi Limit 1200 Agit Hi Agit Lo Limit 250 Agit Lo Advanced Control P Gain 0 20 P Gain 1 00 Gain 4 If you wish to change the high and low limits for Agitation speed use the Agit Hi and Agit Lo selector buttons to open an edit box for each use the touchpad to input a value and press the Enter button to save the new value to memory The Hi Limit may not exceed 1200 and the Lo Limit may not go below 250 5 Press X to return to the 402 Details screen 0 to return to the Main Screen To cascade to Oxygen follow steps 1 4 above but select O2 instead of Agitation To cascade to Agitation O2 follow steps 1 4 above but select Agit O2 instead of Agitation Cascades for Cell Culture without Gas Mix Controller For Cell Culture without the Gas Mix Controller you have the same two choices explained for Fermentation without the Gas Mix Controller None or Agitation See Section 7 6 1 above If you have the optional TMFC module see Section 14 for details Cascades for Cell Culture with Gas Mix Controller Wh
85. denser Optional mounted in headplate Filter 0 2u absolute filter resterilizable 11 4 1 Non Jacketed Vessel Dimensions for Autoclaving Vertical Non Jacketed Vessel Assembly with supplied vertical stand amp as prepared for autoclaving Total Vessel Volume 1 3L 3 0L 7 5L 14 0L Height without Exhaust Condenser 16 5 in 16 5 in 19 5 in 24 in to top of bearing housing 42 cm 42 cm 49 5 cm 61 cm Diameter without Exhaust Condenser 8 5 in 8 5 in 11 5 in 11 5 in 22 cm 22 cm 29 cm 29 cm continued New Brunswick Scientific User s Guide 128 Non Jacketed Vessel Assembly with supplied vertical stand amp as prepared for autoclaving Total Vessel Volume 1 3L 3 01 7 5L 14 0L Height with Exhaust Condenser 22 in 22 in 25 5 in 29 in to top of bent over exhaust filter 56 cm 56 cm 65 cm 74 cm Width with Exhaust Condenser 8 5 in 8 5 in 11 5 in 11 5 in 22 cm 22 cm 29 cm 29 cm Length with Exhaust Condenser 9 5 in 9 5 in 14 5 in 11 5 in 24 1 cm 24 1 cm 37 cm 29 cm The above dimensions do not include optional addition bottles or optional sampler which may be mounted to the vessel assembly for autoclaving they can also be autoclaved unattached If addition bottles are attached add the following approximate dimensions to vessels without exhaust condenser gt 4cm to diameter gt 8 cm to height of 1L
86. dplate Figure 22 1 3L Headplate EXHAUST HARVEST SAMPLER SPARE ASSEMBLY RTD THERMOWELL SEPTUM Each bolt is a possible mounting position for a bottle holder New Brunswick Scientific User s Guide 38 Figure 23 3 0L Headplate HARVEST TUBE EXHAUST Mounting position for Sampler Assembly SAMPLE RTD THERMOWELL LEVEL PROBE dO2 PROBE TRI PORT Each bolt is a possible mounting position for a bottle holder BioFlo 110 Modular Fermentor 1273 0054 User s Guide 39 Figure 24 7 5L amp 14 0L Headplate MOUNTING POSITION FOR SAMPLER ASSEMBLY LIFTING HANDLE 402 PROBE PLUG 6 35mm SPARE LIFTING HANDLE RTD THERMOWELL TRI PORT REPE PLUG 19mm SPARE PLUG 12mm SPARE Each bolt is a possible mounting position for a bottle holder New Brunswick Scientific User s Guide 40 4 6 2 4 6 3 Install Headplate Clamping Ring The clamping ring that secures the headplate to the vessel is split in half to facilitate installation under the vessel flange They are joined with two rectangular mounting plates As shown in Figure 25 below install one mounting plate with two Phillips head screws provided on the end of one ring half so that the plate extends beyond the ring Figure 25 Installing Headplate Clamping Ring 69 Q O O In the same manner install the second mounting plate on the other end of the ring h
87. e Ingold polarographic 1 3L P0720 6281 dO2 Probe Broadley James polarographic 1 3L P0720 6370 dO2 Probe Ingold polarographic 3 0L P0720 6282 dO2 Probe Broadley James polarographic 3 0L P0720 6371 dO2 Probe Ingold polarographic 7 5L P0720 6283 dO2 Probe Broadley James polarographic 7 5L amp 14 0L P0720 6372 dO2 Probe Ingold polarographic 14 0L P0720 6284 Foam Level Probe Kit 1 3L M1273 9947 Foam Level Probe Kit 3 0L M1273 9951 Foam Level Probe Kit 7 5L amp 14 0L M1273 9960 pH Probe Kit Ingold gel filled 1 3L M1273 9970 pH Probe Kit Ingold gel filled 3 0L M1273 9977 pH Probe Kit Ingold gel filled 7 5L M1273 9982 pH Probe Kit Ingold gel filled 14 0L M1273 9985 pH Probe Ingold gel filled 1 3L P0720 5582 pH Probe Broadley James gel filled 1 3L 0720 5741 pH Probe Ingold gel filled 3 0L 0720 5584 pH Probe Broadley James gel filled 3 0L 0720 5747 pH Probe Ingold gel filled 7 5L 0720 5580 pH Probe Broadley James gel filled 7 5L 0720 5742 pH Probe Ingold gel filled 14 01 P0720 5583 pH Probe Broadley James gel filled 14 0L P0720 5743 RTD Assembly all vessels M1273 8019 New Brunswick Scientific User s Guide 122 10 2 10 3 Sampler System NBS Part Number Sampler Kit 1 3L M1273 9946 Sampler Kit 3 0L M1273 9949 Sampler Kit 7 5L M1273 9953 Sampler Kit 14 0L M1273 9956 Syringe 60 mL P0440 0054 Disposable Filter P0200 0970
88. e 1 3L 3 0L amp 7 5L vessel baffle see Section 4 6 21 If you are using a 14 0L vessel install the baffle assembly inside the glass vessel 1 Gently compress the baffle ring at its ends to avoid scratching the vessel walls You may find it convenient to squeeze the tab with your thumb 2 Slide the assembly inside with the tab facing up until it comes to rest at the bottom of the vessel 3 Orient the baffle so the opening is opposite the gradations on the vessel 4 6 5 Install Impeller s Install the impeller s as follows New Brunswick Scientific User s Guide 42 79 NOTE A For Cell Culture Slide the impeller onto the agitation drive shaft from the bearing housing Position the impeller at least 10 mm above the sparger Clamp it down in place It is normal for the agitation impeller shaft to be very resistant to turning by hand This resistance ensures sterile operation B For Fermentation Slide one impeller onto the agitation drive shaft from the bearing housing Position this lower impeller according to the table below Clamp it down in place Then install the second upper impeller in the same manner Distance from Bottom of Headplate to Top of Impeller Blade 1 3L 3 0L 7 5L 14 0L Lower Impeller 4 1 8 in 6 11 16 in 8 7 8 in 12 in 105 mm 170 mm 225 mm 305 mm Upper Impeller 2 5 8 in 4 in 675 in 974 in 67 mm 102 mm 165 mm 235 mm 79 NOTE The distan
89. e 152 14 5 SPEGIEICATIONS EN E i dass A t E N E E 153 14 6 TROUBLESHOOTING n op r E Lei cde tree oi E eie EP 153 15 APPENDIX E DRAWINGS eessececssecesssecsscceesseceessecsscoecssccecsseceessecessoecsscoceesseceessecsssoeessoceesseceessecsssseessoceess 154 15 1 5 e a te ee tuted a recette ied e EE eec eo 154 15 2 DRAWINGS EE EE E EAEE eme ot EEE E iere ee reed 162 16 APPENDIX F PORTS amp ADAPTERS eeeseseesseceessecsscoccssocecsseceessecescoeessocecsseceesoecssccecsseceeseceessecsssseesseceese 164 17 INDEX 165 BioFlo 110 Modular Fermentor M1273 0054 User s Guide 1 OVERVIEW 1 1 Design Concept The BioFlo 110 is a modular fermentation system for microbial and cell culture applications It features a modern easy to use control interface plus an available family of vessels controller modules and other accessories that address the diverse needs of the bioprocessing community A complete range of water jacketed and non jacketed fermentation and cell culture vessels is available to cover 0 4 through 14 0 liters For temperature control non jacketed vessels use an external heating blanket and an internal cooling coil Jacketed vessels use an external jacket water temperature controller Modularity means you can position components to suit you
90. e the headplate insert the level probe into the appropriate port see Figures 11 12 amp 13 for reference 2 Finger tighten the knurled adapter 4 5 14 Install Addition Tube s Insert addition tubes and or tri ports in the appropriate ports for any or all of the following additions media nutrients acid base antifoam For each insertion 1 Finger tighten the knurled addition or tri port adapter 2 Working from above the headplate insert the addition tube or tri port into the appropriate port see Figures 11 12 amp 13 for reference 4 5 15 Install pH Probe 79 NOTE Prior to installation any pH probe you are using should be inspected for damage and replaced if necessary Y amp NOTE To avoid damage to the probes during operation be sure that there is no interference between the probes and the baffle assembly impeller blades or cooling coil New Brunswick Scientific User s Guide 26 1 Wear protective gloves to protect yourself in case of accidental breakage 2 Lightly coat the pH probe with glycerol CAUTION Do not install the pH port adaptor in the headplate before inserting the probe Follow the steps below to fit the pH port adapter onto the probe first then insert the probe and adapter into the headplate Figure 15 pH Probe with Port Adapter exploded pH PROBE ADAPTER y top portion TOP FERRULE BOTTOM FERRULE TN lz 7 PROBE ADAPTER bottom portion
91. ececeescececececececececececececececececececs 93 7 6 SETTING THE PH LOOP 35 setti Fees a GR Ea BS 93 7 6 1 Setting a Deadband for 1 94 7 6 2 Setting the Output Multiplier for pH Control eese enne eene nennen nenne 95 7 6 3 Setting P amp I Gains for pH Control sees enne enne s trennen 97 7 1 SETTING THE DO2 ds 98 7 8 SETTING DO2 CONTROL CASCADES ccccccecesececesececececececeeecececececececececececececececececececececececececececececececececececs 98 7 8 1 Cascades for Fermentation without Gas Mix Controller eee 98 7 8 2 Cascades for Fermentation with Gas Mix Controller 100 7 8 35 Cascades for Cell Culture without Gas Mix Controller seen 102 7 8 4 Cascades for Cell Culture with Gas Mix Controller esee 102 7 9 SETTING P amp I GAINS FOR DO2 1 00 000 105 7 10 SETTING THE GAS MIX FOR FERMENTATION cccccscececececececececececececececececececececececececececececececececececececececs 106 7 11 SETTING THE GAS MIX FOR CELL 444 444444 106 7 11 1 2 107 7 11 2 De
92. eller shaft see Section 4 4 8 4 1 14 0L Vessel Disassembly Special care must be taken when you remove the headplate assembly from the 14 0L vessel because the vessel narrows at the neck 1 After you remove the headplate nuts and washers lift the headplate assembly a few inches and hold it up with one hand 2 With the other hand reach into the vessel squeeze the baffle between thumb and forefinger tightly enough to clear the vessel neck 3 Lift the assembly straight up until the entire assembly is clear 8 4 2 Vessel Cleaning Methods There are three ways to clean your vessel New Brunswick Scientific User s Guide 116 Method 1 1 Drain all the liquid from the vessel 2 Disassemble and wash the headplate glass vessel impellers baffles and other components in hot water and an appropriate laboratory detergent 3 If necessary use a very small test tube brush to clean the ports and orifices A soft brush may also be used to remove dried on material from the glass 4 Rinse all components in tap water 5 Rinse twice in purified deionized RO distilled water Allow everything to dry thoroughly before reassembling the vessel Method 2 1 Drain the liquid from the vessel 2 Fill the vessel to the maximum working volume with water 3 Reassemble the vessel and components and reconnect the agitation shaft to the motor 4 Press the Agitation key On 5 After approximately ten minute
93. en through automatically via P amp I Gains see Section 7 9 below controlled agitation speed and oxygen output This is how it functions when the actual dO2 value rises above the dO2 setpoint the agitation speed will automatically decrease until the dO2 setpoint is reached Conversely when the actual dO2 value drops below the setpoint the cascade system acts to bring it back up Oxygen controls dissolved oxygen by automatically adjusting the mix of air and oxygen This is not available without the Gas Mix Controller Agitation Oxygen controls dissolved oxygen by automatically running Agitation to the maximum speed then if dissolved oxygen still has not reached the setpoint increasing the oxygen flow through the sparger This cascade is the most frequently used in fermentation This is not available without the Gas Mix Controller The default setting is Cascade To None which means that dissolved oxygen will be controlled only by the addition of oxygen when the actual dO2 level drops below setpoint BioFlo 110 Modular Fermentor M1273 0054 User s Guide 101 To cascade dissolved oxygen to Agitation 1 In the dO2 Details screen press the Setup selector button The Setup screen opens J dO2 73 1 A Defaults Cascade To None Cascade Agit Hi Limit 1200 Agit Hi Agit Lo Limit 250 Agit Lo 1 Advanced Control P Gain 0 20 P Gain l Gain 1 00 Gain 2 Press the Cascade selector button The Casca
94. en you are using the Gas Mix Controller you have four choices to cascade to for Cell Culture Agitation 4 Gas Demand or None If you have the optional TMFC module see Section 14 for details Cascading to e Agitation controls dissolved oxygen through automatically via P amp I Gains see Section 7 9 below controlled agitation speed and oxygen output This is how it functions when the actual dO2 value rises above the dO2 setpoint the agitation speed will automatically decrease until the dO2 setpoint is reached BioFlo 110 Modular Fermentor M1273 0054 User s Guide 103 Conversely when the actual dO2 value drops below the setpoint the cascade system acts to bring it back up e 4Gas controls dissolved oxygen by automatically adjusting the feed of all gases introduced to the culture This is not available without the Gas Mix Controller e Demand controls dissolved oxygen by automatically adjusting the feed of carbon dioxide nitrogen and oxygen This is not available without the Gas Mix Controller e The default setting is Cascade To None which means that dissolved oxygen will be controlled only by the addition of oxygen when the actual dO2 level drops below setpoint To cascade dissolved oxygen to Agitation 6 In the dO2 Details screen press the Setup selector button The Setup screen opens dO2 73 1 96 Defaults Cascade To None Cascade Agit Hi Limit 300 Agit Hi Agit Lo Limit 25 Agit Lo Advanced
95. er connections have been made the jacket is filled with water the vessel is filled with media and the RTD has been installed in the thermowell and connected to the Power Controller Never plug the jacket water heater into the controller before the vessel is filled When the heater is connected properly and the PCU is turned on the jacket water stirrer should spin freely WARNING When the jacket water heater is plugged in do not touch the top surface It is very hot BioFlo 110 Modular Fermentor M1273 0054 User s Guide 83 XA NOTE Always unplug the jacket water heater when not in use Whenever you remove the vessel be sure to turn the temperature control Off refer to Section 7 4 1 and unplug the jacket water heater If you do not the jacket water heater could reach excessive temperatures posing a burn danger to personnel and causing the heater s internal safety cutoff switch to interrupt power to the heater If the safety cutoff is activated power will not be restored until you do the following 1 Wait for all surfaces to cool 2 Turn the jacket water heater over and press the Thermostat Reset button located on the bottom see Figure 42 below 3 Reinstall the vessel jacket filled with water and vessel with media temperature probe in the thermowell and plugged into the Power Controller Figure 42 Jacket Water Heater Bottom View RESET BUTTON ELECTRICAL SPECIFICATION ET LABEL 2
96. er s Guide 61 Plug the following into the front panel of the dO2 pH Controller e The dissolved oxygen probe cable 102 The pH probe cable right connector marked pH and the pH probe ground left connector marked pH Attach the ground clip to the top of a headplate screw Level Controller If you are using the BioFlo 110 Level Controller familiarize yourself with the connections and controls on the front panel as illustrated in Figure 37 below Figure 37 Level Controller Front Panel LEVEL f aono no BIOFLO 110 Foam Probe Wet On level sensor Wet Off level sensor Plug the following into the front panel of the Level Controller e Foam probe left connector marked Foam When this probe gets wet its associated pump turns on e Wet On level sensor middle connector marked Wet On When this probe gets wet its associated pump turns on e Wet Off level sensor right connector marked Wet Off When this probe gets wet its associated pump turns off 4 10 4 Pumps If you are using the BioFlo 110 4 Pump Module familiarize yourself with these features see Figure 38 below e ON OFF SWITCH An individual power switch for each pump is located on the front panel e PUMP DOOR Behind each hinged door is access to a peristaltic pump New Brunswick Scientific User s Guide 62 Figure 38 4 Pump Module Front View OFF OFF 9
97. erial number Drive Power Switch 25 pin l On 9 pin Recorder O Off Connector Connector Power Inlet 5 165 Connectors daisy chain to Fuse next module Holder Refer to Figure above to visually locate the following features on the rear panel e FLOPPY DRIVE An internal A drive for field upgrades to the interface software e POWER SWITCH On Off rocker switch e AC CONNECTION Inlet for power cord supplied either for connection to Power Controller outlet or for electrical service outlet e FUSE HOLDER Easy access to replace 250V 2 amp Slo Blo fuse New Brunswick Scientific User s Guide AFS MODBUS 25 pin D connector for any supervisory software you CONNECTOR may add e CONTROLLER BUS Two RS 485 9 pin D connectors one above the other CONNECTORS Use one to connect the Power Controller to the PCU with the cable supplied e RECORDER One 9 pin D connector reserved for use with any analog CONNECTOR recorder you may add 2 5V output supplies data records 4 1 2 Power Controller Figure 2 Power Controller Rear Panel PERISTALTIC PUMP CONNECTIONS 3 assignable by operator CONNECTOR for WATER SOLENOID VALVE CABLE x INDICATOR uS LIGHTS UNIT ADDRESS SELECTION REFERENCE TABLE POWER OUTLET Connect to PCU FUSE 4 PIN UNIT ADDRESSS with supplied DRAWER SELECTOR power cord LOCATION POWER INLET Connect to Mains with supplied power cord RS 485 CONNECTORS da
98. ew 4 6 12 Install Level Probe s If you are using a level probe as part of the antifoam system and or a level probe to detect media level one at a time 1 Working from above the headplate insert the level probe into the appropriate port see Figures 22 23 amp 24 for reference 2 Finger tighten the knurled adapter 4 6 13 Install Addition Tube s Insert addition tubes and or tri ports in the appropriate ports for any or all of the following additions media nutrients acid base antifoam For each insertion 1 Working from above the headplate insert the addition tube or tri port into the appropriate port see Figures 20 21 amp 22 for reference 2 Finger tighten the knurled addition or tri port adapter 4 6 14 Install pH Probe 79 NOTE Prior to installation any pH probe you are using should be inspected for damage and replaced if necessary Y amp NOTE To avoid damage to the probes during operation be sure that there is no interference between the probes and the baffle assembly or impeller blades 1 Wear protective gloves to protect yourself in case of accidental breakage 2 Lightly coat the pH probe with glycerol BioFlo 110 Modular Fermentor M1273 0054 User s Guide 45 CAUTION Do not install the pH port adaptor in the headplate before inserting the probe Follow the steps below to fit the pH port adapter onto the probe first then insert the probe and adapter into the headplate Fi
99. ew Brunswick Scientific R Recorder Ouput Pin Designations for 72 Register Addresses 142 Register Map 142 Replacement Part Numbers Bearing Housing Cap 122 Miscellaneous 122 O Ring Kit 122 Replacement Parts Addition Harvest Kit 122 Angled Autoclave Rack 120 Batteries 120 Exhaust Condenser 120 Foam Trap Kit 120 Fuses 120 Headplate Port Fittings 120 Impellers 120 Motors 120 Part Numbers 119 Probes amp Sensors 121 Sparger 122 Tubing 121 Vessel Assemblies 119 Vessels 122 Replacing Batteries 132 Replacing Bearing Housing Cap 117 Replacing Fuses 117 Replacing Headplate O Ring 117 Replacing Port amp Adapter O Rings 116 Request for Data 140 Request Format 141 RTD 24 43 RTD Connection 60 Sampler Installation of 30 Installing the 50 Sampler Tube Installation of 24 43 Sampling 112 Schematics 4 Pump Module 159 dO2 pH Controller 156 Gas Mix Controller 158 Level Controller 157 Thermal Mass Flow Controller 161 Service 118 Setting Setpoints 98 105 Setting the Pumps 109 Sparger Installation of 23 42 Part Numbers 122 Specifications 4 Pump Module 126 Antifoam Level Controller 126 Dimensions for Autoclaving 127 128 129 dO2 pH Controller 125 Gas Mix Controller 126 User s Guide Non Jacketed Vessels 127 PCU 124 Power Controller 125 153 Spin Filter Option Part Numbers 122 Stacking Modules 10 Sterilization Preparing for 76 Sterilization Temper
100. gainst the vessel walls 3 Align the headplate holes with the vessel stand pillars then slide it down until it rests securely against the vessel flange 4 Finger tighten each clamping nut a little at a time to secure the headplate on the vessel stand working diagonally from one to another rather than working around the circle to apply equal pressure See important NOTE on following page BioFlo 110 Modular Fermentor M1273 0054 User s Guide 35 N CAUTION To avoid vessel stress cracks especially during autoclaving never overtighten vessel clamping nuts 4 6 Vessel Assembly Water Jacketed One of the most versatile features of the BioFlo 110 is the wide variety of glass vessels available There are two types of vessels non jacketed which are provided with the basic models and water jacketed Each type of vessel is available in four sizes 1 3 liters 3 0 liters 7 5 liters and 14 0 liters maximum working volume for more detail see Specifications in Appendix A If you have a non jacketed vessel and have already completed Section 4 5 above skip down to Section 4 7 Water jacketed vessels need no stand the water jacket which is part of the vessel is flared and flat at the bottom to provide secure stable support At the bottom is a metal base plate to provide additional security against breakage In operation the jacked vessel sits on the Jacket Water Heater The jacket water heater is designed so that t
101. gth of stiffening material to the tubing using rubber bands or tie wraps to support the filter Figure 30b Exhaust Condenser 14 0L Vessel Only STERILE FILTER TIT Lil W Lo SET SCREW _ in port adapter a EXHAUST PORT New Brunswick Scientific User s Guide 50 4 6 17 Install Sampler The optional BioFlo 110 sampler system is designed to aseptically remove batch samples from the vessel The entire installation is easily autoclaved in place on the vessel If you are using the sampler install the kit as follows using Figures 31 and 31b for reference 1 Remove a headplate clamping nut adjacent to the location of the sampler tube 2 Mount the metal sampler bottle holder on the clamping screw and secure it in place with the clamping nut Figure 31a Sampler Harvest System 1 3L Vessel THUMB CLAMP SYRINGE FILTER ES Mi ma SYRINGE 1 8 SILICONE TUBING REDUCING ELBOW to 3 16 tubing on sampler holder SAMPLER xU HARVEST PORT J SAMPLER BOTTLE V HOLDER SAMPLER BOTTLE HEADPLATE CLAMPING NUT HEADPLATE CLAMPING SCREW SAMPLE TUBE HARVEST TUBE BioFlo 110 Modular Fermentor M1273 0054 User s Guide 51 Figure 31b Sampler System 3 0L 7 5L amp 14
102. gure 27 pH Probe with Port Adapter exploded 7 CAP pH PROBE ADAPTER top portion TOP FERRULE Dp BOTTOM FERRULE TN M33 pH PROBE ADAPTER bottom portion TEFLON O RING white a PORT O RING black With reference to Figure 27 above 3 Gently slide the top portion of the knurled port adapter part of the probe kit onto the probe 4 Slide the two white ferrules onto the probe the narrower one on top of the deeper cup shaped one 5 Gently slide the bottom portion of the port adapter onto the probe taking care to orient the longer threaded section toward the top of the probe 6 Remove the two O rings installed in the pH port first slide the white Teflon O ring onto the probe then follow with the black 12mm port adapter O ring 7 Do not yet close up all the elements of the port adapter 8 Gently insert the probe into the appropriate port see Figures 22 23 amp 24 for reference allowing the O rings to seat fully into the port YT amp NOTE The fit may be snug Gently rotate the probe as you press it into the port to avoid breakage 9 Finger tighten the bottom portion of the port adapter into the port New Brunswick Scientific User s Guide 46 10 Adjust the probe to the desired height then nesting the ferrules close the top portion of the adapter onto the bottom portion 11 Finger tighten the knurled adapter assembly 4 6 15 Install Dissolved Oxygen Probe 2 NOTE Pr
103. he appropriate addition port on the headplate Remove the clamp Making Connections Power Confirm that the voltage selection of your BioFlo 110 matches the voltage rating of your electrical service by reading the voltage selection in the window of the fuse drawer see Figure 45 Also confirm that the power requirements of all control modules marked on each module s serial number sticker are met by your electrical service Plug in the Power Controller connect power cord P0720 2053 to the Power Controller s power inlet Do not plug into your electrical service until you are ready to run the unit Plug in the Primary Control Unit PCU 1 support a single vessel assembly connect power cable 0720 2054 to the Power Controller s rear panel power outlet labeled PCU and plug the other end into the PCU s rear panel power inlet 2 Tosupport multiple vessel assemblies with multiple Power Controllers either a follow step 1 above choosing the most convenient Power Controller or b use the supplied power cord to plug the PCU directly into your electrical supply thus making it independent of the Power Controllers Water The BioFlo 110 water solenoid valve is located on the side of the Power Controller Water pressure should be 10 PSIG maximum with 50 um filtration New Brunswick Scientific User s Guide 58 Be sure to secure all tubing connections for both the water and the drain lines with the fibergla
104. he bottle placed on the floor below the benchtop to the harvest tube outlet Secure both the tubing and the filter with plastic ties 2 Aseptically install the harvest tubing on the harvest tube outlet with a thumb clamp on the tubing Close the clamp Secure the tubing on the harvest tube with a plastic tie 3 Set the harvest bottle on the floor below the vessel 4 There are two ways to prime the harvest process a with a pump or b without a pump a Thread the tubing through one of the peristaltic pumps unclamp the tubing and manually turn the pump on Harvesting will begin b Install a rubber sampler bulb on the other end of the harvest bottle filter Unclamp the harvest tubing then squeeze and release the bulb Harvesting will begin 5 Once harvesting has been primed there are two ways to complete the process a Allow the pump to operate until the vessel is exhausted b Allow gravity to do the work Note that a small amount of residue will probably remain in the vessel no matter how you harvest New Brunswick Scientific User s Guide 114 8 MAINTENANCE Preventive maintenance keeps your equipment in proper working condition When performed routinely maintenance results in longer life for your equipment It also reduces time lost due to equipment failure A WARNING Always disconnect the power cord from the Power Controller and the PCU before performing maintenance Never immerse any BioFlo 110 mod
105. he instrument or part thereof which shall with in 1 year after date of shipment prove to be defective after our examination This warranty does not extend to any NBS products which have been subjected to misuse neglect accident or improper installation or application nor shall it extend to products which have been repaired or altered outside the NBS factory without prior authorization from the New Brunswick Scientific Co Inc BioFlo 110 Modular Fermentor M1273 0054 User s Guide vii New Brunswick Scientific DECLARATION OF CONFORMITY New Brunswick Scientific Hereby declares that the product s listed below conform to the European Union directive and standards identified in this declaration Product s BF110 Thermal Mass Flow Controller module M1273 3109 EU Directive s Low Voltage 73 23 EEC 93 68 EEC Electromagnetic Compatibility 89 336 EEC 93 68 EEC Standard s IEC61010 1 1990 1 1992 2 1995 EN61010 1 1993 2 1995 EN61326 1997 A1 1998 The conformity assessment procedures were performed at the following Testing Lab Intertek Testing Services 40 Commerce Way Totowa NJ 07512 The technical documentation relevant to the above equipment will be held at New Brunswick Scientific Company PO Box 4005 44 Talmadge Road Edison New Jersey 08818 4005 U S A Tel 732 287 1200 Fax 732 287 4222 New Brunswick Scientific User s Guide BioFlo 110 Modular Ferme
106. he vessel water inlet and outlet fit in a notch at the rear and the vessel feet fit into the four holes at the perimeter of the heater plate Figure 21 on the following page shows a typical installation of the double walled water jacketed vessel with the most commonly used accessory equipment New Brunswick Scientific User s Guide 36 Figure 21 Water Jacketed Vessel Assembly A on A Y E a m e m A D ji is am T 1 3L 3 0L 14 0L AGITATION MOTOR NOTE This vessel is shown ud installed on the Jacket Water Heater gt BEARING HOUSING p x a LIFTING HANDLE ue HEADPLATE TOP CLAMPING RING PARRE COOLING WATER aon OUTLET TUBE WATER JACKET TX SPARGER _ BOTTOM CLAMPING THERMOWELL mum a qnia an p RING BASE PLATE CY COOLING WATER OUTLET connect to drain COOLING WATER INLET connect to water solenoid valve BioFlo 110 Modular Fermentor M1273 0054 User s Guide 37 Familiarize yourself with the arrangement of the headplate ports as shown in the following diagrams before proceeding with the vessel assembly You may find it more practical to change the arrangement the variety of ports and adapters will easily accommodate your needs For easy reference see Appendix E for a mix and match summary of port sizes and the types of adapters and inserts available 4 6 1 Hea
107. ides New Brunswick Scientific User s Guide 64 Bolt on cable guide clamps are provided to dress probe and sensor cables from the front of their control modules toward the back of the stack where they naturally hang from the top of the vessel Their use is optional You may prefer to tuck the cables under their module directing them toward the back of the stack or to simply allow them to hang The cable guides can be mounted on either side of each module wherever they will best suit your needs They can also be mounted with the top jaw facing up or down The two jaws of the guide snap together so the tip of the top jaw zips along the tracks on the bottom jaw to expand or contract the cable enclosure The guide functions rather like a plastic tie with the difference that it can be opened and closed repeatedly As shown in the drawing below to open a clamp 1 Place your thumb and forefinger on either side of the top jaw at the boss 2 Grip tightly and pull up 7 Because the guide clamps down tightly you to encourage unclamping with the tip of a screwdriver under the leading edge Do not use undue force however to avoid damage to the plastic clamp or if already present to the cable inside Figure 40 Cable Guide ran TOP JAW Location of Bolt A 4 BOTTOM JAW 4 12 Voltage Selection and Fusing 79 NOTE If you move your BioFlo 110 to a new locati
108. ighten all nuts 9 Reinstall the addition foam trap and or harvest tubing in the appropriate pump s and release the clamp s 10 Reconnect the air and or gas lines 11 Add 1 2 ml of glycerin to the thermowell and reinstall the RTD temperature probe Connect the cable to the Power Controller WARNING If the temperature probe is plugged into its controller but not inserted into the thermowell the vessel will overheat resulting in risk to the operator and to the culture New Brunswick Scientific User s Guide 80 6 5 Confirming pH Calibration Autoclaving can alter the zero characteristics of pH probes typically by 0 1 to 0 3 pH To check and to compensate for any discrepancy you will need an accurate external pH meter If one is not available skip this section 1 Following sterilization with the media at room temperature note the pH value on the BioFlo 110 Main screen 2 Take a sample of media and measure the pH on the external meter 3 If the two values disagree return to the BioFlo 110 Calibrate pH screen and set Zero to the value reported by the external meter Do not change the Span or you will invalidate the entire calibration The pH value will now agree with the external meter s reading 6 6 Polarizing amp Calibrating the dO2 Probe 7 If you using polargraphic dO2 probe it must be polarized after autoclaving and before calibration Polargraphic dissolved oxygen pro
109. iliarize yourself with the connections and controls on the front panel of the Power Controller as illustrated in Figure 35 below POWER SWITCH On Off rocker switch OPERATING LIGHT Lights when the Power Controller is on AGITATION Connection for agitation motor CONNECTOR BioFlo 110 Modular Fermentor M1273 0054 User s Guide 59 e TEMPERATURE e HEATER CONNECTOR Connection for RTD temperature probe to sense temperature inside the vessel Connection for the heat blanket non jacketed vessels or for the jacket water heater water jacketed vessels Figure 35 Power Controller Front Panel RTD HEATER WATER JACKET SONETON TEMPERATURE SENSOR TEMPER TURE 2 MOTOR HEATER AGITATION DRIVE 5 ane 9998 A 4 snurswicx NE POWER SWITCH ROTAMETER Keon Os ott CABLE mount on OPERATING GUIDE either side LIGHT mount on either side Note the following features and connections on the side of the Power Controller e ROTAMETER e WATER INLET amp OUTLET New Brunswick Scientific Can be mounted on either side Manually adjusted rotameter regulates the flow of air gases into the vessel Via the water solenoid valve located on the side panel which provides the connection between the water supply and the vessel water inlet either cooling coil
110. ings to seat fully into the port 7 NOTE The fit may be snug Gently rotate the probe as you press it into the port to avoid breakage 9 Finger tighten the bottom portion of the port adapter into the port 10 Adjust the probe to the desired height then nesting the ferrules close the top portion of the adapter onto the bottom portion 11 Finger tighten the knurled adapter assembly 4 5 17 Install Exhaust Condenser A WARNING Never intentionally block the exhaust to raise vessel pressure If you are using the optional exhaust condenser 1 Unscrew the spare exhaust port plug from the headplate saving it for reuse 2 Place the 12mm exhaust condenser adapter into the port 3 Place the exhaust condenser inlet see Figures 18a amp 18b below into the port and finger tighten the knurled adapter 4 Tighten it with the Allen key provided until it is secure 5 Attach the exhaust filter respecting the direction of flow if stamped on the filter to the condenser outlet Secure the filter with a plastic tie 6 Connect silicone tubing to the inlet port of the exhaust condenser Secure with a plastic tie 7 Connect the other end of the tubing to the water inlet see NOTE below and Figure 17 on the following page split from the water solenoid valve on the side of the Power Controller Secure with a plastic tie BioFlo 110 Modular Fermentor M1273 0054 User s Guide 29 79 NOTE The water solenoid valve s
111. ior to installation any dissolved oxygen 402 probe you using should be inspected for damage and replaced if necessary 7 NOTE To avoid damage to the probes during operation be sure that there is no interference between the probes and the baffle assembly or impeller blades 1 Wear protective gloves to protect yourself in case of accidental breakage 2 Lightly coat the dO2 probe with glycerol CAUTION Do not install the dO2 port adaptor in the headplate before inserting the probe Follow the steps below to fit the dO2 port adapter onto the probe first then insert the probe and adapter into the headplate Figure 28 dO2 Probe with Port Adapter exploded CAP AN dO2 PROBE ADAPTER top portion TOP FERRULE BOTTOM FERRULE Y L j dO2 PROBE ADAPTER bottom portion p TEFLON O RING white PORT O RING black BioFlo 110 Modular Fermentor M1273 0054 User s Guide 47 With reference to Figure 28 above 3 Gently slide the top portion of the knurled port adapter part of the probe kit onto the probe 4 Slide the two white ferrules onto the probe the narrower one on top of the deeper cup shaped one 5 Gently slide the bottom portion of the port adapter onto the probe taking care to orient the longer threaded section toward the top of the probe 6 Remove the two O rings installed in the dO2 port first slide the white Teflon O ring onto the probe then follow with the black 12mm port adapter O
112. ip of the plastic lock from left to right then gently lift up toward you The drawer will pop out 3 Pull the entire drawer assembly out and set it aside 4 Use the screwdriver blade to pull the selector cube out from the left side insert the blade into the hollow of the cube 5 Turn it 180 to display 115 then snap it back into place Be sure to press it firmly until it snaps 6 Reinsert the fuse drawer and snap it into place New Brunswick Scientific User s Guide 134 79 NOTE If you are using pumps with or without the Pump Module you will need to replace them with pumps configured for the new voltage 79 NOTE Although the voltage selector is located in housing termed fuse drawer it contains no fuses The fuse drawer contains a spring and a blank Be sure to keep these intact and in place when you reinstall the fuse drawer 12 4 Changing PCU Fusing If you are changing the voltage on your Power Controller it may also be necessary to modify the fusing arrangement on the PCU 79 NOTE Be sure to discuss this with your NBS representative before a qualified technician makes any modifications to the unit 12 4 1 Converting from Single Fuse to Double Fuse Your BioFlo 110 PCU was initially set up with a single fuse arrangement To change to a double fuse 1 Open the cover of the power module using a small blade screwdriver and remove the cover fuse block assembly see Figure 47 below 2 Loose
113. isy chain to other modules RS 485 CABLE cap unused connector provided with Terminator plug BioFlo 110 Modular Fermentor M1273 0054 User s Guide Refer to Figure 2 above to visually locate the following features WATER SOLENOID VALVE WATER SOLENOID CONNECTOR VOLTAGE SELECTOR AC CONNECTION PCU CONNECTION CONTROLLER BUS CONNECTORS UNIT ADDRESS SELECTOR Located on the side panel not visible in drawing Provides water from outside source for cool temperature control within the vessel and to the optional exhaust condenser Located on the rear panel Control connection for the water solenoid valve Located in the fuse drawer in the rear panel next to the power cord connection Located on the rear panel Input connection using the power cord supplied to the electrical service Located on the rear panel Output connection using the power cord supplied to power the PCU Located on the rear panel Two RS 485 9 pin D connectors one above the other Use one to connect the Power Controller to the PCU with the cable supplied Located on the rear panel Four two position on off switches used to identify the vessel address uniquely associating this module to one particular vessel dO2 pH Controller Figure 3 dO2 pH Controller Rear Panel Serial Number Reference Table for Unit Address Selector CONTROLLER BUS Q Unit Address Selector Location Module Serial
114. itional Preparation for Autoclaving To continue preparing the vessel for sterilization gt AR pes Remove the motor from the top of the vessel and carefully put it aside Lubricate the vinyl bearing housing cap with silicone grease to facilitate sliding the cap securely onto the housing Place the bearing housing cap on the top of the bearing housing Disconnect the air and or gas lines from the inlet filter on the sparger Disconnect the water lines Remove all PVC tubing Clamp off the harvest tube the sample tube and all other penetrations that are immersed in the media Remove the RTD from the thermowell Disconnect all probes and sensors and remove their cables If you are using pH and dO2 probes install each probe s shorting cap provided in the probe kit BioFlo 110 Modular Fermentor M1273 0054 User s Guide 77 10 11 12 Before placing the vessel into the autoclave loosen the glass sample bottle by gt turn Wrap all filters with aluminum foil to protect them from steam Attach a piece of tubing wrapped with some non absorbent material such as glass wool or non absorbent cotton to one of the addition ports Wrap foil around the end of the tubing shaped like a funnel to allow the vessel to vent more easily during autoclaving Place a clamp on the tubing 79 NOTE Be sure to leave one clamp open during autoclaving to equalize pressure If this is a water jacketed vessel als
115. jacent to the base The two AA alkaline batteries are housed in the battery holder at the front of the base Cut the plastic tie wrap Remove then replace the batteries taking care to align them properly the polarity is marked in the bottom of the battery holder Replace the tie wrap this is optional Ease the cover back in place lowering it straight down until it fits tightly Take care to tuck the ground wire and the ribbon cables inside clear of both the cover and the lip of the base Reinstall the side mounting screws Tighten securely AN WARNING Always disconnect the power cord from the Power Controller and PCU before undertaking any of the following procedures BioFlo 110 Modular Fermentor 1273 0054 User s Guide 133 12 3 Voltage Selection 79 NOTE If you move your BioFlo 110 to a new location that requires a change in voltage and or fusing be sure to consult your NBS representative Locate the fuse drawer on the back panel of the Power Controller next to the power plug The voltage selection is displayed in a small window in the middle of the fuse drawer The Power Controller is preset at 115 Volts If the voltage needs to be changed to 230V 1 Using a narrow tipped flat bladed screwdriver introduce the blade into the small slot to the left of the fuse drawer Figure 46 Voltage Selection Insert screwdriver tip here Fuse Voltage Fuse Drawer Lock 2 Use the blade to pry the l
116. l pH 6 68 7 00pH Auto dO2 77 1 0 0 Auto The Temperature Loop Details screen appears see sample below s Temp 23 4 3 Control Off Control Output 100 0 Setpoint Disp Hi Disp Lo New Brunswick Scientific User s Guide 90 If the Control mode is Off the PCU is not controlling temperature To turn the control mode on e Press the selector button next to Control Current Control Press here to change Mode Control Mode Temp 23 4 Control Off m Control Setpoint Disp Hi Disp Lo Output 100 0 Control Off Off Output 100 0 Auto e Press the selector button next to Auto The control mode is automatically updated as the Temperature Loop Details screen returns see sample screen below BioFlo 110 Modular Fermentor M1273 0054 User s Guide 91 j Temp 23 4 H Setpoint Disp Hi Disp Lo 4 0 7 4 2 Setpoint To set the temperature setpoint AWOL 1 Press the selector button next to Setpoint A highlighted edit box appears Temp 28 7 C Control Auto Control Output 100 0 Disp Hi Disp Lo 2 Using the numeric touchpad enter the new setpoint New Brunswick Scientific User s Guide 92 Temp 28 7 2 Control Auto Control F Output 100 0 32 0 o Disp Hi Disp Lo 1 0 3 Press
117. l Antifoam System If you are using a level probe to activate the addition of chemical defoamer install the antifoam addition bottle following the procedure for installing a foam trap with the following differences e Install the bottle holder close to the antifoam addition tube in the headplate e Connect the longer tube that penetrates the bottle cap to the antifoam addition tube in the headplate 4 6 20 Plug Unused Ports Close off all unused ports 1 Install a blind plug without a hole in any headplate port that will not be used 2 Install silicone tubing secured with a plastic tie and clamped shut on any access tube 1 harvest tube that will not immediately be used 79 NOTE It is good practice to lightly lubricate the headplate O ring with silicone before installing it on the vessel 4 6 21 Install 1 3L 3 0L or 7 5L Fermentation Vessel Baffle 1 Gently place the baffle tab facing up around all of the other instruments protruding from the headplate 2 Position the tab and baffle opening toward the back of the headplate A CAUTION Hold the baffle in place with two fingers when you lift the headplate assembly New Brunswick Scientific User s Guide 54 4 6 22 Install Headplate 1 If this is a 1 3L 3 0L or 7 5L fermentation vessel be sure to squeeze and hold the baffle in place opening toward the back with thumb and forefinger You may find it convenient to squeeze the tab with your thumb
118. llowed to wet and block the filter surface D To protect susceptible vessel assembly components from steam damage New Brunswick Scientific User s Guide 76 6 1 6 2 The first objective is met by leaving at least one vessel port open the second by clamping shut flexible tubing attached to immersed penetrations and the third by wrapping filters with a protective cap of aluminum foil Use protective caps on probes and bearings to meet the fourth objective Initial Preparation for Autoclaving If this is a water jacketed vessel the jacket must be half filled with water prior to autoclaving Follow the steps in Section 6 1 1 below If this is a non jacketed vessel proceed to Section 6 2 Filling the Water Jacket To fill the water jacket 1 After the tubing and water supply are connected make sure the solenoid valve cable and the RTD cable are plugged into the Power Controller Set the temperature control mode to Off for instructions see Section 7 4 1 Check that the temperature reading is higher than 5 C Allow water to enter the piping system it will stop at the solenoid valve Set the temperature loop control mode to Auto Enter a temperature setpoint SP that is at least 12 C below the current value CV The controller will respond to the call for cooling by opening the solenoid valve filling the jacket with water 7 When the jacket is halfway filled set the temperature control mode to Off Sv Uto Add
119. mand Mode seu rer RETE E C ANM LEE E ERG SEEN Ee EX REE 107 7 11 3 100 ee 107 7 12 SETTING THE PUMPS 108 7 12 1 Pump 108 7 12 2 Pump Setpoint and 108 2 12 3 CONE OL IARE 109 7 12 4 Setting Pumps RUE per UR EPI bee euer resp ERN US 109 7 13 SETTING THE PUMP CONTROL MODE ANTIFOAM WET ON amp WET 110 7 14 INOCUEXATIONES ee tee ee Pee Pee Ear IE PEE EHE VER Pee iN 112 7 15 yu MINA QE GARRETT TI OER ERE RAS 112 7 16 AXUTOMATIC FOAM CONTROL ug 112 7 17 eter NEED UE ER ERE C EO ates eR TAOTE PUENTE ERREUR CENE 113 256825006500 rE 114 8 1 CLEANING THE MODULES cccccccocccccecescccsecoscccescoscscececscscsecesesssesescscecevscecssevsvevsvevevasavevsveveveveveseveveveveves 114 8 2 PH PROBE MAINTENANCE AND 5 tetr reren rtr e r ese e ese ese ese s ese e ese esas erennere 114 8 3 DO2 PROBE MAINTENANCE AND 5 114 8 4 88 6 255 p Or DIOE 115 8 432 Vessel
120. mounting arm Secure the holder in place with the clamping nut Leave the nut loose enough to swivel the holder Firmly place the foam trap bottle 250 ml or 500 ml in the holder BioFlo 110 Modular Fermentor M1273 0054 User s Guide 33 5 With the bottle cap in place aseptically install a sterile 0 2 u filter on the shorter tube that penetrates the cap Be sure to respect the proper flow direction if stamped on the filter 6 Connect a length of silicone tubing to the longer tube in the other bottle cap penetration Secure the tubing with a plastic tie and clamp it off on the top 7 Connect the tubing securing it with a plastic tie to the foam exhaust tube in the headplate 8 After autoclaving you will position the bottle holder where you want it then finger tighten the clamping nut Figure 20 Foam Trap 2 SILICONE TUBING STERILE FILTER 3 UM FOAM TRAP BOTTLE 250 or 500 ml FOAM EXHAUST TUBE HEADPLATE NI WA 7 7 vESSEL BOTTLE HOLDER BOTTLE HOLDER MOUNTING ARM HEADPLATE CLAMPING NUT 4 5 20 Plug Unused Ports Close off unused ports 1 Install a blind plug without a hole in any headplate port that will not be used 2 Install silicone tubing secured with a plastic tie and clamped shut on any access tube 1 harvest tube that will not immediately
121. mpellers Installation of 21 41 Part Numbers 120 Important Warnings 14 Inoculation 112 Inspection of Boxes 3 of Equipment 3 Interconnecting Modules 12 International Offices List of iii Introduction 1 Jacket Temp Control 60 Jacket Water Heater 35 82 Reset Button 82 Safety Cutoff 82 Jacket Water Heater Connection 60 L Language Choosing the 69 Level Probe s Installing the 25 44 Location Environment for 4 Physical 4 User s Guide Preparing the 4 Maintenance 114 Manual Conventions v Message Formats 141 ModBus Settings for 138 Module Kits Replacement Part Numbers 123 Motors Part Numbers 120 Multidrop Number 140 NOTE Symbol for v O Option Part Numbers Air Filter Regulator Kit 122 Spin Filter 122 Water Filter Regulator Kit 122 Optional Accessories Part Numbers 122 O Ring Kit Replacement Part Numbers 122 O Ring Replacement 116 Overview 1 P amp I Gains Control Equation for pH 136 Part Numbers Accessories 122 Module Kits 123 Port Adapters 164 pH Control Setting a Deadband 94 Setting an Output Multiplier 95 Setting P amp I Gains 97 pH Probe Calibrating the 65 94 Installation of 25 44 Port amp Adapter O Ring Replacement 116 Power Back Up 84 Probes amp Sensors Part Numbers 121 Pt 100 60 Pump Control Mode 109 Pump Flow Rates 126 Pump Module 9 Pump Multiplier 108 Pump Operation 108 Pump Setpoint 108 N
122. n 7 9 Setting P amp Gains for dO2 Control 79 NOTE We recommend that you do not change the factory P amp I settings unless you are an advanced user of P amp algorithms P and I values are numbers that determine how the fermentor responds to changing growth conditions and new setpoints Because growth characteristics of bacterial and cell cultures are very different they require some different P and I values for proper control For example for cell culture the P value should be 2 00 and the I value 0 67 while the factory set values for fermentation are P 0 20 and I 1 00 Incorrect P and I values can cause poor control which may actually mimic component failure For this reason we recommend that you use the default factory set P and I gains If you are very experienced with P and I algorithms and wish to change the default values use this procedure 1 In the dO2 Loop Setup screen press the P Gain selector button 2 Using the touchpad enter the new P Gain value in the highlighted edit box 3 Press the Enter key to save the new value or press X to cancel The new value appears onscreen 4 Press the Gain selector button A highlighted edit box will open 5 Using the touchpad enter the new Gain value 6 Press the Enter key to save the new value or press X to cancel The new value appears onscreen 7 After the values have been set press the gt button to return to the Main Screen Anytime you
123. n the Phillips screw behind the fuse block 2 full turns see Figure 48 below 3 Remove the fuse block by sliding it up and away from the screw shaft and lifting it off the pedestal see Figure 48 below 4 Invert the fuse block and slide it back onto the Phillips screw and pedestal Tighten the Phillips screw BioFlo 110 Modular Fermentor 1273 0054 User s Guide 135 Figure 47 Fuse Holder Power Inlet USE ONLY WITH 250V FUSE DISCONNECT POWER BEFORE FEPLACIRG FUSE Figure 48 Fuse Block Cover Assembly FUSE FUSE BLOCK COVER 5 Verify the correct fusing arrangement see Figures 49a and 49b below 6 Insert fuses 7 Replace the cover unit into the fuse holder New Brunswick Scientific User s Guide 136 Figure 49a Double Fusing Arrangement Figure 49b Single Fusing Arrangement COVER 12 5 P amp I Control Equations 79 NOTE The following information is provided for the use of advanced P amp I algorithm users only 12 5 1 pH As mentioned in Section 7 6 3 following is the P amp I control equation for pH 100 SP CV 100 Out P 14 14 f SP CV dt i s Se vos Where P is dimensionless and I is in min BioFlo 110 Modular Fermentor M1273 0054 User s Guide 137 12 5 2 dO2 As mentioned in Section 7 9 following is the P amp I control equation for dO2 10096 SP CV 1 10096 100402 100402 Out SP CV dt
124. n the pH Control screen A multiplier of 50 for example has the base pump run for 5 seconds every 10 seconds if the pH controller is calling for base addition BioFlo 110 Modular Fermentor M1273 0054 User s Guide 109 7 12 3 7 12 4 Control Mode Pumps A B and C accept a user assigned Control Mode The available control modes are Manual Wet On Wet Off and Antifoam Wet On Wet Off and Antifoam are the designations given for convenience to the probes that are plugged into the socket of the same name on the Level Module Control modes affect pump operation in the following ways e Manual pump operates at the setpoint e Wet On when the probe is immersed in media or foam the pump turns on at its assigned setpoint This is useful to remove media when additions accumulate to an undesired level for example e Wet Off when the probe is not immersed media or foam the pump turns on at its assigned setpoint This is useful for media addition Antifoam when the probe is immersed in media or foam the pump turns on at its assigned setpoint This is useful to add chemical defoamer Setting Pumps To set the Multiplier for pH pumps Acid and Base see Section 7 6 2 Be sure also to set the Control Mode Setpoint and Graphic Display Hi and Lo limits for those pumps Following the instructions below 1 Set the Control Mode 2 Enter a Setpoint 3 Select the Hi and Lo limits of the Graphic Display presented in the Lo
125. n the same manner Distance from Bottom of Headplate to Top of Impeller Blade 1 3L 3 0L 7 5L 14 01 Lower Impeller 4 1 8 in 6 11 16 in 8 7 8 in 12 in 105 mm 170 mm 225 mm 305 mm Upper Impeller 2 5 8 in 4 in 675 in 974 in 67 mm 102 mm 165 mm 235 mm 79 NOTE The distances indicated above provide a recommenced starting point As working volumes and agitation rates change you may wish to adjust the impeller location s 79 NOTE It is good practice to lightly lubricate all O rings port threads and adapter threads with silicone grease before you install equipment in the headplate Also inspect the headplate O ring to be sure it is securely seated in its groove BioFlo 110 Modular Fermentor M1273 0054 User s Guide 23 4 5 6 Install Cooling Coil 1 3L Vessel Cooling Coil Sparger Assembly The cooling coil and sparger connections are welded into one special 12mm tri port assembly 1 From beneath the headplate insert the assembly into the appropriate ports see Figure 11 2 From above the headplate lock the assembly in place with a knurled 12mm to 12mm adapter Finger tighten 3 There are three set screws in the adapter If you need to raise or lower the adapter tri port assembly use the Allen key provided to adjust the set screw that is easiest to access You only need to adjust one 3 0L 7 5L amp 14 0L Vessel Cooling Coil 1 From beneath the headplate in
126. nd calibrate the dO2 probe see Section 6 6 7 8 Setting dO2 Control Cascades Cascading brings several systems together to work jointly to achieve your goal There are four possibilities for Fermentation and four other choices for Cell Culture if you are using the Gas Mix Controller Without the Gas Mix Controller there are two cascades available to either Fermentation or Cell Culture In all cases when you first set up the system the default choice is set to None no cascade 7 8 1 Cascades for Fermentation without Gas Mix Controller For Fermentation you have two choices when you are not using the Gas Mix Controller None or Agitation If you have the optional TMFC module see Section 14 for details Cascading to Agitation is designed to control dissolved oxygen through automatically controlled agitation speed and oxygen output via P amp I Gains see Section 7 9 below This is how it functions when the actual dO2 value rises above the dO2 setpoint the agitation speed will automatically decrease until the dO2 setpoint is reached Conversely when the actual dO2 value drops below the setpoint the cascade system acts to bring it back up The default setting is Cascade To None which means that dissolved oxygen will be controlled only by the addition of oxygen when the actual dO2 level drops below setpoint BioFlo 110 Modular Fermentor M1273 0054 User s Guide 99 To cascade dissolved oxygen to Agitation 1 In
127. ns set the O2 Mix in Auto Mode as reflected in the resulting screen Gas 0 0 SLPM Control Control Off Setpoint Setpoint 0 0 Output 0 0 O2 Mix Auto O2 Mix Output 0 0 Set 02 3 The default gas flow setpoint is 20 If you wish to change it select Setpoint then type in the desired value from 0 to 20 SLPM the system will automatically limit the gas setpoint to 20 4 Select Set 2 to set the percentage of oxygen 74 NOTE After you set the 2 the PCU will provide the complementary percentage of air For example if you set 2 at 25 the PCU will provide 75 air BioFlo 110 Modular Fermentor M1273 0054 User s Guide 149 4 Gas 0 0 SLPM Control F Control Off Setpoint Setpoint 18 0 Output 0 0 1 1 0 O2 Mix Auto O2 Mix Output 100 0 Set 2 5 Finally press the Control selector button then set the Gas loop control mode to Auto You will hear the solenoid click and gas will come through the sparger YT amp NOTE It is important to set the O2 Mix and 2 before turning the Gas loop control on to avoid a sudden burst of large bubbles into the vessel 14 3 2 Fermentation Mode Cascades Before you set cascades first review the table at the top of Section 14 3 to identify the cascades available according to the combination of modules in your system If you have the TMFC without the Gas Mix Module the 402 Cascade screen will look like this ul dO2 73 1
128. nsert it making sure it is well seated in the groove 8 7 Bearing Housing Cap Replacement Check the bearing housing cap after each use for distortion tears or any other damage to its integrity Replace the cap as needed 8 8 Replacing PCU Fuses To replace a fuse in the PCU A CAUTION Always disconnect the unit from the power source first Remove the cover fuse block located on the rear of the unit with a small screwdriver Remove the old fuse Insert a new fuse of the same type Replace the cover fuse block into the Fuse Holder Power Inlet see Figure 45 below TR Figure 45 Fuse Holder Power Inlet FUSE BLOCK gt PAY USE ONLY WITH COVER 250V FUSE DISCONNECT POWER BEFORE REPLACIAG FUSE New Brunswick Scientific User s Guide 118 9 SERVICE If any problems occur with your BioFlo 110 system or its individual components do not attempt to perform any service on it Unauthorized servicing may void the warranty Please contact your local NBS Service Department or your local NBS distributor In any correspondence with NBS please refer to the Model Number BioFlo 110 Manufacturing Part Number s and Serial Number s of the problem module This information is located on the bottom of each unit 9 1 Troubleshooting If no information appears on the display check the following before you call for service 1 Verify that the power cord is correctly connected to the Power Contr
129. nst the rubber bumpers 4 Orient the vessel so the gradations on the glass are clearly visible at the front facing the user and situated between two vessel stand pillars 4 5 4 Install Baffle 14 0L Fermentation Vessels ONLY For installation of the 1 3L 3 0L and 7 5L vessel baffle see Section 4 5 21 If you are using 14 01 vessel install the baffle assembly inside the glass vessel 1 Gently compress the baffle ring at its ends to avoid scratching the vessel walls You may find it convenient to squeeze the tab with your thumb 2 Slide the assembly inside with the tab facing up until it comes to rest at the bottom of the vessel New Brunswick Scientific User s Guide 22 3 Orient the baffle so the opening is opposite the gradations on the vessel and the tab is aligned with the back vessel stand pillar 4 5 5 Install Impeller s Install the impeller s as follows A For Cell Culture Slide the impeller onto the agitation drive shaft from the bearing housing Position the impeller at least 10 mm above the sparger Clamp it down in place 79 NOTE It is normal for the agitation impeller shaft to be very resistant to turning by hand The shaft seal resistance ensures sterile operation B For Fermentation Slide one impeller onto the agitation drive shaft from the bearing housing Position this lower impeller according to the table below Clamp it down in place Then install the second upper impeller i
130. nstall Dissolved Oxygen Probe tre ee erii pee ie eee tto 46 4 6 16 Install Exhaust Condenser 8 47 4 6 17 Install ram EE 50 4 6 18 T stall Fo m Trap eus c ree ERR rS ee EDT ERE ios e REDE bre 52 4 6 19 Install Antifoam System era be Ra Dre ee RES ter Peer o toe RE RES eR Re EL stay 53 4 6 20 Plus Unused 4 eiie PUERO e RETE SEI Re Pee pe a ANEA 25 4 6 21 Install 1 3L 3 0L or 7 5L Fermentation Vessel Baffle eese enne 53 4 6 22 Install Headplate is scd ee ERR e EIE EUM Yo Yee EGENT HERR 54 4 7 INSTALE THE AGITATION eee ves senses eve vea nep eue v a eva ye eee eee avos ve VERE 54 4 8 INSTALL THE ROTAMETRBR erret eene ees eee be POR ee en vue eda eve ee vacesgeaveeseervereus 55 4 9 INSTALL LIQUID ADDITION SYSTEMS ener 56 4 10 MAKING A yen Pee eee vea e PARE V 57 4 10 1 A E EEE 57 4 10 2 WLC 37 4 10 3 Probes Gd Sensors isset ee REGERE ENTER 58 4 10 4 M H E 61 4 10 5 Air Gnd Optional Gases n Et E bec HO o Ey HER lve De HE
131. ntor M1273 0054 User s Guide TABLE OF CONTENTS 1 OV ERV DEW 6 oseitiscssessacssssscccscscecssesseccssuavscoassseekcastesessusteceseaueeaseessescustesoespedecdssouceveusacesssbncsveetavesos stacecsssuavessnsessoecs 1 1 1 DESIGN GCONGEPT 1 1 2 PROCESS CONTROL e is v e e ere eet i eee 1 1 3 COMPUTER READY 5 icri 2 14 ENHANCING Y OUR FERMENTOR cssssssssccececessssscecececeessnsenecececscnsnnaececeesceessaaeaecececsensnasaeeececsessnaaaeseesceense 2 2 INSPECTION amp UNPACKING ivcssissssisccscessecdssssssscsessceossestcassdectsosdncicoscbeceisesoasscossbecdentbcsssuessossdsbbansscbees setees es 3 2 1 INSPECTION OF BOXES paseo She cance cages eh idee erecto eee PUT EINER WU e ae o PR E 3 22 PACKING LIST VERIFICATION 5 3 3 225 02052 2 5545 o era ono ero rea eese eaae ek reae eU eo Fees Ua 4 3 1 PHYSICAE I OCASION E caves tat utat teet Eae sies 4 3 2 ENVIRONMENT 575 tma ohh ee ate EE 4 3 3 baits Ss EE 4 4 INSTALLATION ASSEMBLY amp SET UP cssssscsssssccsssscccsssscccssssccccs
132. o 6 35mm tube To make 12mm port universal for M1273 5056 COMPRESSION ADAPTERS Compression adapters allow you to easily raise or lower the probe or tube while maintaining a safe seal 6 35mm port to 4 76mm For Sample Tube Foam Probe Level M1273 5042 tube Probe 12mm port to 4 76mm tube To make 12mm port universal for M1273 5055 12mm 6 35mm amp 4 76mm diameters 12mm port to 12mm tube For dO2 amp pH probes M1273 5040 BioFlo 110 Modular Fermentor 1273 0054 User s Guide 165 1 100 Gas Feed Setting up 107 4 4 Gas Mode 107 A Accessories Part Numbers 122 Addition Harvest Kit Part Numbers 122 AFS Communications Protocol 139 AFS Supervisory Software Settings for 138 AFS ModBus Pin Connections 139 Agitation Motor Connection 60 Air Filter Regulator Kit Installation of 63 Part Numbers 122 Angled Autoclave Rack Part Numbers 120 Antifoam 110 Application Choosing the 86 Autoclave Caps Replacement Part Numbers 122 Autoclaving Preparing for 76 Autoclaving Cap Replacement 117 Autoclaving Dimensions 127 Autoclaving the Vessel 77 Automatic Gas Mix Overriding the 107 Automatic Gas Sequencing Setting the 107 B Baffle Installation of 21 34 41 53 Base Plate Installing Vessel on 40 Batteries Part Numbers 120 Battery Back Up 84 Battery Replacement 132 Bearing Housing Cap Replacement Part Numbers 122 Bearing Housing Cap Replacement 117 BioC
133. o leave the jacket water inlet clamp open 6 3 If you have addition foam trap or harvest bottles mounted at the base of the vessel you can autoclave them with the vessel Without detaching their tubing from the headplate 13 14 15 16 Remove the bottle holder s and reinstall each one of the headplate clamping screws Reinsert the bottle and turn the holder until the bottle and holder are positioned over the headplate rather than extended over the edge Finger tighten the knurled nut Clamp off the tubing and where appropriate remove it from the pump Probe tips must be moist during sterilization If you will be doing batch fermentation be sure the vessel is filled with media so the media will also be sterilized If you will be doing cell culture use at least 100 ml of a balanced salt solution such as phosphate balanced saline solution Sterilize the media separately after autoclaving the vessel Autoclaving the Vessel 1 Ifyou have a 7 5L or 14 0L vessel assembly that is too tall for your autoclave carefully lay the vessel still mounted in its stand if present in the optional angled autoclave rack part number M1273 9266 see Figure 41 below Secure it in place with the strap 24 Insert the entire vessel assembly glass jar vessel stand if present headplate and all headplate components into an autoclave and sterilize Do not sterilize the jacket water heater When you remove the vessel fr
134. oller and to an outlet of appropriate output If the PCU is powered via the Power Controller make sure the power cord is correctly connected between both modules If not verify that the power cord is correctly connected to the PCU and to an outlet of appropriate output Verify that the Power Controller s power switch is turned On Confirm that the PCU power switch is turned On Check that all control modules are correctly connected to each other Make sure that all cables are attached properly Verify that each module s unit address selector switches are correctly assigned per Section 5 2 BioFlo 110 Modular Fermentor M1273 0054 User s Guide 119 10 REPLACEMENT PARTS amp ACCESSORIES When you order replacement parts accessory parts or when you request service information please provide the Model Number BioFlo 110 Manufacturing Part Number s and Serial Number s of your unit This information is located on the bottom of each module 10 1 Replacement Parts Vessel Assembly NBS Part Number Non Jacketed FERMENTATION VESSEL ASSEMBLY glass vessel headplate clamping nuts baffle thermowell RTD probe O rings port plugs amp adapters exhaust tube sparge tube harvest tube direct drive motor amp agitation shaft bearing housing set of bearing housing caps rotameter 2 six bladed Rushton type impellers include cooling coil heater blanket vessel stand and water valve Jacke
135. om the autoclave immediately crimp the foil funnel on the addition port and close off the vent tubing to maintain sterility New Brunswick Scientific User s Guide 78 Figure 41 Angled Autoclave Rack Option for 7 5L amp 14 01 Vessels EXHAUST CONDENSER Non jacketed VESSEL ASSEMBLY BEARING HOUSING CAP VESSEL STAND FOAM TRAP and or ADDITION BOTTLES AUTOCLAVE RETENTION RACK STRAP 6 3 1 Sterilization Time and Temperature Sterilization time varies with autoclave characteristics temperature settings vessel size and contents i e media properties As a starting point autoclave for 25 minutes after the autoclave reaches 121 C CAUTION During autoclaving the vessel and the water jacket if present must be vented at all times Release autoclave pressure only when the temperature has dropped below 90 C Use slow exhaust 30 60 minutes Autoclave should be on liquid cycle pressure release if it has such a feature 79 NOTE Filter manufacturers generally advise limiting filter sterilization to 30 minutes but the longer time required for slow exhaust is essential to protecting the vessel integrity NBS long experience has shown no adverse effects at all on filters exposed to longer autoclaving times Adjust the time and temperature as needed If you have a water jacketed vessel and the jacket is not half filled the vessel may not reach sterile temperature BioFlo 110 Modular Fermentor 1273 0054
136. ommand Communications Protocol 139 New Brunswick Scientific 17 INDEX Bottom Clamping Ring Installing the 40 C Cascade To 4 Gas 102 104 Agitation 98 100 101 102 103 Agitation O2 100 102 Demand 102 104 None 98 100 102 2 100 102 Cascades for Cell Culture 102 for Fermentation 98 100 CAUTION Symbol for v CE Declaration of Conformity vii Change Command 139 141 Change Command Acknowledgement 141 Checklist for Basic Microbial Kit 3 Choosing the Application Cell Culture 86 Fermentation 86 Choosing the Language 69 Cleaning the Modules 114 the Vessel 115 Comml 138 Comm 1 Settings 138 Comm2 138 Comm2 Settings 138 Communications Protocol for AFS BioCommand 139 for ModBus 141 Communications Settings 138 Connections 12 Control Equation for pH P amp I Gains 136 Control Type Values 142 Cooling Coil Installation of 23 Copyright Notice v D Data Logging 72 Demand Mode 107 Dimensions for Autoclaving 127 Disclaimer Notice v Dissolved Oxygen See dO2 dO2 Control Setting Cascades for 98 100 102 Setting P amp I Gains 105 dO2 Probe User s Guide Calibrating the 81 Installation of 27 46 Polarizing the 80 dO2 Setpoint Setting the 98 105 Drawings 1 3L Headplate 18 37 3 0L Headplate 38 7 5L amp 14 0L Headplate 20 39 Cable Guide 64 dO2 pH Controller Front 60 dO2 pH Controller Rear 7 Exhaust Condenser 48 49 Foam Trap 52 Fuse Block Co
137. on that requires a change in voltage and or fusing consult your NBS representative BioFlo 110 Modular Fermentor M1273 0054 User s Guide 65 It is possible to change the voltage on your BioFlo 110 It is also possible to change the fusing arrangement from a single fuse to a double fuse These procedures however should only be performed by a qualified technician referring to Appendix B in this manual 4 13 Calibrating the pH Probe If you are using a pH probe be sure to calibrate it before autoclaving the vessel assembly 1 Check that the Power Controller and PCU are plugged in and powered on 2 Make sure that the pH electrode is plugged into the pH connector on the dO2 pH Controller front panel using the appropriate cable Also check that the pH ground wire is plugged into the adjacent ground connector and the ground clip is grounded 3 When the PCU is powered on the display says New Brunswick Scientific BioFlo 110 Scanning Hardware 4 When the system detects the hardware the display shows the following screen English NBS BioFlo 110 Francais Version 0001 01 Config 1 10 Deutsch Date 2 2000 Espanol http www nbscicom NOTE This is a sample Use screen Your specific Version Config amp Date More may differ SELECTOR BUTTONS The default language setting is English If you prefer to use another screen language press the selector button next to
138. ondenser 11 5 in 11 5 in 11 5 in 11 5 in 29 cm 29 cm 29 cm 29 cm A CAUTION Never autoclave the jacket water heater Unplug and remove it The above dimensions do not include optional addition bottles or optional sampler which may be mounted to the vessel assembly for autoclaving they can also be autoclaved unattached If addition bottles are attached add the following approximate dimensions to vessels without exhaust condenser gt 4 to diameter gt 8 cm to height of 1L 3L vessels gt 10 cm to height of 7 5L 14L vessels e Addition bottles add no height to vessels with condenser e Sampler adds approximately 4 cm to diameter 11 4 4 79 NOTE Non Jacketed Vessel Dimensions for Autoclaving Inclined For use with an Angled Autoclave Rack the attachment of addition bottles is not recommended New Brunswick Scientific User s Guide 130 Water Jacketed Vessel Assembly with optional Angled Autoclave Rack M1273 9266 amp as prepared for autoclaving Total Vessel Volume 7 5L 14L Height with Exhaust Condenser 18 5 in 47 cm 20 5 in 52 cm Length with Exhaust Condenser 26 in 66 cm 28 5 in 72 cm Width 11 5 in 29 cm 11 5 in 29 cm A CAUTION Never autoclave the jacket water heater Unplug and remove it BioFlo 110 Modular Fermentor 1273 0054 User s Guide 131 12 APPENDIX B TECHNICAL INTERVENT
139. op Details screen for each assignable Pump A B and C To set up the first peristaltic pump Pump A under the PCU s supervision 1 In the Main Screen press the selector button in the Pump A row to open this screen New Brunswick Scientific User s Guide 110 0 0 Control Off Control 0 i 0 Setpoint o Disp Hi Disp Lo 0 The procedures are generally the same as for the Temperature and Agitation Loops to set the Graphic Display Hi and Lo parameters the Setpoint and the Control Mode if you wish to choose Manual which allows the operator to manually set pump output or Off For the three automatic Control Modes unique to the pump loops Antifoam Wet On and Wet Off see Section 7 13 below for details 2 Repeat the setup procedure for Pump B and Pump C 7 13 Setting the Pump Control Mode Antifoam Wet On amp Wet Off If your system includes the Level Controller the PCU can supervise the three level loops identified as Antifoam Wet On and Wet Off each of which is actually a control mode for the assigned pump as mentioned in the section above 1 To set up the defoamer pump from the pump s Details screen press the Control selector button for its designated pump The Control screen opens BioFlo 110 Modular Fermentor M1273 0054 User s Guide 111 ul Pump 0 0 i Control Off Off F Output 0 Manual Antifoam Wet On Wet Off 0
140. or water jacket Inlet is marked 1 and outlet 2 User s Guide 60 SPARGE AIR INLET Via the rotameter mounted on the side panel or the Thermal Mass Flow Controller either of which provides the connection between the air gas supply and the sparger Attach all cables to their appropriate probes and sensors then attach the cables to the appropriate module not necessarily in this order Into the front panel of the Power Controller e Plug the RTD temperature probe cable where it is marked Temperature e Plug the agitation motor cable where it is marked Agitation A WARNING Agitation and Heater connection sites are high voltage Never insert anything but the appropriate cable plug You will also plug into the front panel when you are ready to begin operation e For non jacketed vessels the heat blanket cable where it is marked Heater e For water jacketed vessels the jacket water heater cable where it is marked Heater but only after the jacket is filled with water the vessel is filled with media and the vessel is properly installed on the heater dO2 pH Controller If you are using the BioFlo 110 dO2 pH Controller familiarize yourself with the connections and controls on the front panel as illustrated in Figure 36 below Figure 36 dO2 pH Controller Front Panel pH Probe Connector dO2 Probe Connector pH Probe Ground BioFlo 110 Modular Fermentor M1273 0054 Us
141. r workspace and working style The controller modules can be vertically stacked to conserve bench space Modularity also makes it easy and economical to adapt your system for different tasks at any time As an autoclavable fermentor the BioFlo 110 requires an external sterilizing apparatus that is large enough to accept the complete vessel assembly 1 2 Process Control The heart of the BioFlo 110 is the Primary Control Unit PCU The PCU serves as the operator interface for one to four vessels Its bright graphic display and clearly marked keypad serve as the control center for all attached vessels and their associated loop controllers Four channel output to a strip chart recorder is available from the PCU YT amp NOTE No more than 16 modules any combination can be supported by one PCU The BioFlo 110 Power Controller is required for temperature and agitation control It also provides power for liquid pumps and other control modules The rotameter and cooling water valve are mounted on the outside of the Power Controller Additional control modules and sensors enable expanding a basic temperature agitation airflow fermentor to include control of pH dissolved oxygen dO2 antifoam addition removal and addition of nutrients and other liquids and blending inlet air with up to three other gases New Brunswick Scientific User s Guide 1 3 Computer Ready The BioFlo 110 is compatible with both ModBus and New Brunswick Scientific
142. re 58 4 Pump Module Schematics 220V S001 TB001 BRN 2 3 RED 1 RED t C001 M001 TD 2 BLK BLU 4 6 4 YEL YEL 5002 TBOO2 BRN 2 3 RED 4 RED d T C002 M002 23 D 5 BLK E BLU 4 lt 6 YEL YEL 5005 TBOO3 BRN 2 3 RED 7 RED ps ma E C003 M003 uF 8 BLK mm L BLU 4 6 n YEL YEL 5004 004 BRN 2 3 RED 10 RED EB z C004 M004 uF 11 BLK mm BLU 4 6 2 YEL YEL User s Guide 161 Figure 59 Thermal Mass Flow Controller Schematics New Brunswick Scientific User s Guide List of Drawings Figure Caption Page Number 1 PCU Rear Panel 5 2 Power Controller Rear Panel 6 3 dO2 pH Controller Rear Panel 7 4 Level Controller Rear Panel 8 5 Gas Mix Controller Rear Panel 8 6 4 Pump Module Rear View 9 7 Sample BioFlo 110 Installation 11 8 Interconnecting the Modules 13 144 Ya WRONG Handling of Drive Assembly 15 9b CORRECT Handling of Drive Assembly 15 10 Non Jacketed Vessel Assembly 17 11 1 3L non jacketed Headplate 18 12 3 0L non jacketed Headplate 19 13 7 5L amp 14 0L non jacketed Headplate 20 14 Upper Vessel Bumper Installation 21 15 pH Probe with Port Adapter exploded 26 16 dO2 Probe with Port Adapter exploded 27 17 W
143. ring 7 Do not yet close up all the elements of the port adapter 8 Gently insert the probe into the appropriate port see Figures Figures 22 23 amp 24 for reference allowing the O rings to seat fully into the port If you are using an Ingold probe in a 1 3L 3 0L or 7 5L vessel be sure to use the seal washer provided YT amp NOTE The fit may be snug Gently rotate the probe as you press it into the port to avoid breakage 9 Finger tighten the bottom portion of the port adapter into the port 10 Adjust the probe to the desired height then nesting the ferrules close the top portion of the adapter onto the bottom portion 11 Finger tighten the knurled adapter assembly 4 6 16 Install Exhaust Condenser A WARNING Never intentionally block the exhaust to raise vessel pressure If you are using the optional exhaust condenser 1 Unscrew the spare exhaust port plug from the headplate saving it for reuse 2 Place the 12mm exhaust condenser adapter into the port 3 Place the exhaust condenser inlet see Figures 30a amp 30b below into the port and finger tighten the knurled adapter 4 Tighten it with the Allen key provided until it is secure New Brunswick Scientific User s Guide 48 5 Attach the exhaust filter respecting the direction of flow if stamped on the filter to the condenser outlet Secure the filter with a plastic tie 6 With reference to Figures 30a and 30b connect a length of sili
144. s press the Agitation key Off 6 Remove the vessel and disassemble its components 7 Wash all elements in detergent and hot water Method 3 1 Drain the liquid from the vessel 2 Fill the vessel to just above the maximum working volume with hot water and the recommended amount of a suitable detergent 3 Allow the vessel with its component parts to soak for a short time or overnight 4 Drain the vessel 5 Disassemble and wash the headplate glass vessel impellers baffles and other components in hot water and an appropriate laboratory detergent 6 If necessary use a very small test tube brush to clean the ports and orifices A soft brush may also be used to remove dried on material from the glass 7 Rinse all components in tap water 8 Rinse twice in purified de ionized RO distilled water Allow everything to dry thoroughly before reassembling the vessel 7 For easy cleaning the ring sparger has removable plug at the tip 8 5 Port amp Adapter O Ring Replacement Each time you clean the vessel assembly check all port and adapter O rings for wear distortion rips or any other damage to their integrity Replace them as needed BioFlo 110 Modular Fermentor M1273 0054 User s Guide 117 8 6 Headplate O Ring Replacement When it needs to be replaced pull the old O ring out with a knife or a flat bladed screwdriver Lubricate the new O ring with a light coat of silicone grease then i
145. s are possible 4 Gas Demand 100 Feed and Off Selecting Off will turn the Gas Mix Controller off BioFlo 110 Modular Fermentor M1273 0054 User s Guide 107 7 11 1 4 Gas Mode Because your system has the Gas Mix Controller when you select 4 Gas as the control mode the PCU will control the levels of all gases carbon dioxide nitrogen oxygen and air being introduced to the culture to maintain dO2 and pH at their respective setpoints Ji Gas Mix 4 Gas Control 4 Gas Demand Off Gas 2 20 0 100 2 2 49 7 100 2 2 0 0 100 2 Air 30 3 100 Air e cs cus Select this control mode by pressing the 4 Gas selector button There are no further choices to program in this mode the 4 Gas controller will automatically control the sequential introduction of gases according to need 7 11 2 Demand Mode Selecting Demand as the control mode will provide automatic demand sparging of carbon dioxide nitrogen and or oxygen to maintain dO2 and pH at their respective setpoints Press the Demand selector button There are no further choices to program 7 11 3 10096 Feed Mode If you need to feed 100 of any one of the four gases for a period of time you can manually override the 4 Gas controller using this feature To override the Gas Mix Controller s control of gas sequencing in order to feed 100 of one gas in the Gas Mix screen press the selector button next to the gas of choice
146. s missing or fails to operate please fill out Customer Satisfaction Form 6300 packed in the envelope with your warranty card and return it by fax or mail You can also call the New Brunswick Scientific Service Department New Brunswick Scientific User s Guide 3 PREPARING THE LOCATION 3 1 Physical Location The surface where you place the BioFlo 110 should be smooth level and sturdy Ensure that there is enough space around the back and the front of the combined system for proper operation and access 3 2 Environment The BioFlo 110 operates best under the following conditions e Ambient temperature range of 10 C to 35 C e Relative humidity up to 80 non condensing 3 3 Utilities Utility Requirements minimum maximum Water 50 um filtration 5 10 PSIG 0 34 0 68 kg cm Air 3 10 PSIG 0 20 0 68 kg cm Gases optional O2 3 10 PSIG 0 20 0 68 kg cm CO2 3 10 PSIG 0 20 0 68 kg cm N2 3 10 PSIG 0 20 0 68 kg cm Electricity 100V 90 100 VAC 50 60 Hz 10 Amp 120V 108 132 VAC 50 60 Hz 10 Amp 220V 198 242 VAC 50 60 Hz 6 Amp 240V 216 264 VAC 50 60 Hz 6 Amp 79 NOTE gases supplied should be medical grade BioFlo 110 Modular Fermentor M1273 0054 User s Guide 4 INSTALLATION ASSEMBLY amp SET UP 4 1 Introduction to Control Module Rear Panels 4 1 1 Primary Control Unit PCU Figure 1 Primary Control Unit PCU Rear Panel Floppy PCU s
147. s the PCU to a maximum of three vessels Section 2 Inspection amp Unpacking Section 3 Preparing the Location in this manual provide essential information be sure to read them prior to installing this optional module AN WARNING Be sure you are familiar with all warnings and precautions in the body of this manual which are provided for your safety and for the protection of your BioFlo 110 system 14 1 Installation The Thermal Mass Flow Controller TMFC has the same footprint as the Level Controller You can place it on your module stack or beside other modules With reference to Figure 8 Inconnecting the Modules repeated on the following page for reference and Figure 50 TMFC Module Rear Panel daisy chain the TMFC module to the others in your stack Remember that it is powered by the Power Controller and controlled by the PCU so it must be daisy chained New Brunswick Scientific User s Guide 144 Figure 8 Interconnecting the Modules
148. seccesssccccssacccessscccessecsessnscsscsssecesses 5 4 1 INTRODUCTION TO CONTROL MODULE REAR PANELS sssssscsccecsessnececececeessnecesececseseneceeeeeceesenssaeseeeeeenes 5 4 1 1 Primary Contro Umt POU e Sy RH EEE cose aia RE 5 4 1 2 ae nde tet nen Ee 6 Z 1 3 dOZ pHGonitroller ni cate eee ae B petet E I PER eo E ESS 7 4 1 4 Leyel Controllerz sue oc I eei IE 8 4 1 5 Gas MBC Controller 8 41 07 ete bee ite ee tbe 9 427 Other Optional Modules de e m een eee e e rt de eb pereo 10 4 2 STACKING MODULES ER 10 4 3 INTERCONNECTING THE 12 44 TMPORTANT WARNINGS 14 4 5 VESSEL ASSEMBLY NON JACKETED cccccccccesssssscecececcesssececccecseneasesecececeesesaesesececeeseaaeseeeceesenseaeeeeceeenes 16 ADD Headpl te siete eet eet on eta d te tet duties 18 4 5 2 Tnstall Heat Bl nket a dente doct 20 4 5 3 Install Vesselin Vessel Stana 21 4 5 4 Install Baffle 14 01 Fermentation Vessels ONLY
149. sert both ends of the coil into the Cooling Coil In port and the Cooling Coil Out port 2 From above the headplate finger tighten the knurled adapter on each side of the cooling coil 4 5 7 Install Sparger 3 0L 7 5L amp 14 0L Vessels 1 From beneath the headplate insert the sparger tube into the sparger port see Figures 12 amp 13 for reference 2 Finger tighten the knurled adapter on the sparger then use the Allen key provided to tighten the set screw Do not overtighten AN CAUTION Finger tighten only any adapter that has a white Teflon ferrule tapered cone shaped insert under the Teflon washer The ferrule can deform under too much pressure New Brunswick Scientific User s Guide 24 4 5 8 Install Harvest Tube 1 Working from beneath the headplate install the harvest tube in the harvest port see Figures 11 12 amp 13 for reference If you are using the 1 3L vessel the harvest tube and sampler tube are welded into the same tri port to save space When the headplate is in place on the vessel the bottom of the harvest tube should rest at the bottom of the vessel 2 Finger tighten the knurled adapter on the harvest tube then use the Allen key provided to tighten the set screw Do not overtighten 4 5 9 Install Sampler Tube 1 Working from beneath the headplate install the optional sampler tube in the sample port see Figures 11 12 amp 13 for reference If you are using the 1 3L vessel
150. ss reinforced hose clamps provided Attach the inch ID braided PVC tubing provided see NOTE below to the water solenoid valve outlet look for a 2 stamped in the brass valve body just above the hose barb see Figure 17 or 29 and to the vessel cooling coil inlet for non jacketed vessels or the vessel water jacket inlet 7 NOTE There is a distinct visible difference between this PVC tubing and the silicone tubing used elsewhere be careful not to confuse them The PVC tubing is clear with white cross hatched reinforcement braiding Silicone tubing is milky PVC tubing is NOT autoclavable and is not for use with hot liquids it will melt 4 10 3 1 Attach sufficient PVC tubing to the cooling coil outlet for non jacketed vessels or the vessel water jacket outlet to reach the drain 2 Connect to the water supply If you are using the optional exhaust condenser connect tubing from the valve splitter outlet marked 2 on the brass valve body to the exhaust condenser water inlet Connect pressure regulated clean water to the water splitter inlet marked 1 on the brass valve body If you are not using the exhaust condenser connect pressure regulated clean water directly to the inlet of the water solenoid valve marked 1 on the brass valve body 3 If you are using the optional Water Filter Regulator kit see Section 10 3 install it according to the kit instructions Probes and Sensors Power Controller Fam
151. t 4 7 Press the gt key to return to the Main Screen In future should you wish to change an output assignment or to return it to None follow steps 3 5 above to make new selections BioFlo 110 Modular Fermentor M1273 0054 User s Guide 75 6 STERILIZATION amp REINSTALLATION YT amp NOTE Before proceeding consult the dimensions outlined in Sections 11 4 1 and 11 4 2 to be sure your autoclave is large enough to accommodate the vessel with its various components s WARNING During autoclaving the vessel exhaust filter and if present the water jacket drain must be vented to avoid explosion WARNING Use protective gloves when handling hot components N CAUTION During sterilization The bearing housing cap must be installed on the motor bearing housing to keep steam from damaging the internal bearings On water jacketed vessels the jacket must be half filled with water A CAUTION Never autoclave PVC tubing clear with white braiding There are four objectives to preparing a vessel for sterilization A To minimize pressure differences throughout the sterilization process by ensuring that the air can transfer freely between the inside and the outside of the vessel B To ensure that minor pressure differences do not expel liquid from the vessel by clamping off all penetrations that go below liquid level C To protect hydrophobic filters from blockage which would occur if condensation were a
152. tation Gas Flow Oxygen Enrichment Ag Ga O2 or None YES YES Gas In Cell Culture mode the system is configured for dO2 pH control using the NBS gas mixing algorithm see Section 7 9 and separate gas flow control Press the Gas loop selector key to open the Gas Mix screen This screen works as explained in Section 7 11 1 but there is also one additional element Gas Flow control Pressing the Gas Flow selector key allows you to select the gas flow Control mode and Setpoint 0 5 SLPM The dO2 Setup screen shows Gas Flow cascade Hi and Lo limits in addition to the above information Press the appropriate selector keys to change these limits Pressing the Cascade key in the dO2 Setup screen reveals the same dO2 cascade possibilities as with the Gas Mix module alone 4 Gas 4 Gas On Demand Demand Agitation Agit or None see Section 7 8 4 14 3 1 New Brunswick Scientific l Fermentation Mode Setup In the main screen see sample screen on the following page select the Gas loop which represents oxygen and air User s Guide 148 Value Setpoint Control 3 Temp 23 4 300 C l ll Agit 134 350rpm Auto e Pump A 0 0 0 0 96 Manual lt Pump B 00 00 Manual lt iB Pump 0 0 0 0 96 Manual lt M 6 68 7 00pH Auto dO2 77 1 0 0 Gas 0 3 20 0 SLPM Auto Press here 2 In the Gas loop screen that ope
153. ted edit box Do not press Enter yet 5 Establish a reference zero signal from the dissolved oxygen probe using one of the following choices a More accurate Sparge with nitrogen until the dO2 Raw Input stops decreasing b Widely used alternate Momentarily disconnect the dO2 cable from the probe New Brunswick Scientific User s Guide 82 6 When the Raw Input stops decreasing press the Enter key to save the value or press X to cancel 7 Press the Set Span selector button A highlighted edit box opens Calibrate 02 0 Zero Std 0 00 Set Zer 1022 e cs es 8 Using the touchpad key 100 for the new Span value into the highlighted edit box Do not press Enter yet 9 Establish a reference 10046 level of dissolved oxygen in the media sparge with air at the appropriate flow rate for your application 10 When the Raw Input stops increasing press the Enter key to save the 10096 dO2 reference value 12 After the values have been set press the gt button to return to the Main Screen 6 7 Powering the Jacket Water Heater If you have a non jacketed vessel skip this section After sterilization you should have installed the vessel on the jacket water heater and connected the heater to the Power Controller see Section 6 4 Never set the temperature control loop to the desired working temperature refer to Section 7 4 1 until all wat
154. ted vessel assemblies also include jacket water heater 1 3L 3 0L 7 5L 14 01 1 3L 3 0L 7 5L 14 01 CELL CULTURE VESSEL ASSEMBLY glass vessel headplate clamping nuts baffle thermowell RTD probe O rings port plugs amp adapters exhaust tube sparge tube harvest tube motor amp agitation shaft bearing housing set of bearing housing caps rotameter 1 pitched blade impeller Non jacketed vessel assemblies also include cooling coil heater blanket vessel stand and water valve Jacketed vessel assemblies also include jacket water Non jacketed vessel assemblies also M1273 1001 M1273 1002 M1273 1005 M1273 1010 Water Jacketed M1273 1011 M1273 1012 M1273 1015 M1273 1020 heater Non Jacketed with direct drive motor 1 3L M1273 1041 3 0L M1273 1042 7 51 1273 1045 14 01 1273 1050 Non Jacketed with magnetic drive motor 1 3L M1273 1051 3 0L M1273 1052 7 51 M1273 1055 14 0L M1273 1060 Water Jacketed with direct drive motor 1 3L M1273 1021 3 0L M1273 1022 7 5L M1273 1025 14 0L M1273 1030 Water Jacketed with magnetic drive motor 1 3L M1273 1031 3 0L M1273 1032 7 5L M1273 1035 14 0L M1273 1040 New Brunswick Scientific User s Guide 120 Angled Autoclave Rack NBS Part Number For 7 5L amp 14 0L vessels M1273 9266 Batteries amp Fuses NBS Part Number Power Controller Fuse 250V 1 25 Amps P03
155. this reason we recommend that you use the default factory set P and I gains If you are very experienced with P and I algorithms and wish to change the default values use this procedure 1 In the pH Loop Setup screen press the P Gain selector button 2 Using the touchpad enter the new P Gain value in the highlighted edit box 3 Press the Enter key to save the new value or press X to cancel The new value appears onscreen 4 Press the Gain selector button A highlighted edit box will open 5 Using the touchpad enter the new Gain value 6 Press the Enter key to save the new value or press X to cancel The new value appears onscreen New Brunswick Scientific User s Guide 98 7 After the values have been set press the gt button to return to the Main Screen Anytime you wish to return to the default P amp I Gain settings open the pH Loop Setup screen and press the Defaults selector button See Section 12 5 1 for the pH P amp I control equation 7 7 Setting the dO2 Loop When your system includes the dO2 pH Controller the PCU can supervise dissolved oxygen dO2 in the media If you are using a dO2 probe you will need to set the dO2 loop You will calibrate the probe after autoclaving 7 NOTE After autoclaving be sure to calibrate the dO2 probe before setting the dO2 loop If you are using a polarographic dO2 probe it must be polarized before calibration but after autoclaving To polarize a
156. tion 7 8 4 Cleaning See Section 8 1 for instructions on cleaning the module BioFlo 110 Modular Fermentor M1273 0054 User s Guide 153 14 5 Specifications BioFlo 110 Thermal Mass Flow Controller Weight 4 Ib 1 8 kg Dimensions Height 4 in 10 2 cm Depth 12 in 30 5 cm Width 12 in 30 5 cm allow 3 inches 7 6 cm more front and rear for cable access Range 0 100 Input 1 gas input Output 1 gas output Power 24 VDC power transformer supplied Maximum Gas 10 PSI Pressure 14 6 Troubleshooting If no information from the Thermal Mass Flow Controller appears on the display check the following before you call for service 1 Verify that the 24VDC power transformer cable is properly connected to the TMFC and to a grounded electrical outlet 2 Confirm that the TMFC is properly connected daisy chained with RS 485 cables to the Power Controller dO2 pH Controller and the PCU There must be a dO2 pH Controller or the TMFC will not be recognized by the PCU 3 Check that the Power Controller s power switch is turned On 4 Verify that the PCU power switch is turned On 5 Verify that the TMFC s unit address selector switches are correctly assigned per Section 5 2 YT amp NOTE If gas Output which is now controlled by the TMFC does not stabilize check the levels in your supply tanks New Brunswick Scientific User s Guide 154 15 APPENDIX E
157. to open the Systems Details screen see sample screen above 2 Press the selector button next to BaseAdd The Base Address screen will open System Details 0 Unit Base Address 0 4 Unit 1 ID 0 8 Unit 2 ID 1 12 Unit 3 ID 2 16 Unit 4 ID 3 20 Comm Protocol AFS 24 Recorder Status Off 28 3 Press the selector button for the number you wish to assign to the first unit The system will automatically assign the next consecutive numbers to the other units you may be running from this PCU In this way if you choose to give Unit 1 the address of 8 for example Unit 2 will be automatically assigned 9 Unit 3 will be 10 and Unit 4 will be 11 79 NOTE Regardless of the actual number of fermentors being used all four addresses are occupied by the PCU Do not assign any other fermentor in your system a multidrop address within the range used by the PCU 4 Press X key to return to the Systems Detail screen or press the gt key to return to the Main Screen 5 3 4 Set Chart Recorder for Data Logging You may choose to use an optional analog data recorder which you connect via user supplied cable to the PCU s 9 pin male D recorder connector Four outputs per PCU are available to send data to the supervisory system for logging Following are the pin designations for those four outputs BioFlo 110 Modular Fermentor M1273 0054 User s Guide Data Logging Pin Outputs
158. ts presence skip to Section 14 3 Operation If you are adding the TMFC module to a pre established BioFlo 110 system 1 In the PCU s floppy drive see Figure load the update disk shipped with the TMFC 2 After the system boots then follow any instructions that appear on the display screen 3 When the initial setup screen appears see sample on the following page you are ready for operation New Brunswick Scientific User s Guide 146 English amp NBS BioFlo 110 Fran ais lt Version 0001 21 h Config 1 13 Deutsch lt Date 1 2004 Espa ol lt nbsc com NOTE This is a sample lt screen Your specific zi Version Config amp Date may his lt differ The Version should T be 0001 20 or higher aad 14 3 Operation Before proceeding through the following instructions be sure to familiarize yourself with the PCU display selector buttons and the various interface screens described in Section 7 Operation of this manual With a dO2 pH Controller is present in the system there are four possible controller combinations that determine the operation of the eighth loop on the fermentor as recapped in the following table Module s Present 8th Loop Operation Gas Mix TMFC NO NO N A Only option for dO2 control is a cascade to the Agitation loop YES NO O2 As explained in the Operation section of this manual NO YES Gas Pressing the selector ke
159. ttings for the loop or parameter associated with that Field Numeric values in Fields must be ASCII decimal format Requests amp Response Headers Request T Request Format Response Headert Get Controller Outputs MD RE CR Output Sp Sp Get Control Modes MD RJ CR Control Sp Sp MD RL CR X Main Sp Unit Allcontrollers must respond to these four requests or they will not be recognized by BioCommand Headers must contain a total of 9 characters Multidrop ID Number CR Carriage Return Sp 2 Space BioFlo 110 Modular Fermentor M1273 0054 User s Guide 141 13 2 4 Change Commands Change Command Command Headert MS Sp Sp Sp Sp Sp Sp Sp MO Sp Sp Sp Sp Sp Sp Sp All Headers must contain a total of 9 characters Sp Space 13 2 5 Message Formats Response and Change command message format MD Header S Field 1 S Fiela2 S Field 3 Field 9 S Field 10 9 chars p 6 chars p 6chars p 6 chars 6 chars Headers must contain a total of 9 characters Fields must contain a total of 6 characters MD Multidrop ID Number Chars Characters Carriage Return Request format MD Request CR 2 chars MD Multidrop ID Number Chars Characters Carriage Return Change command acknowledge format MD Multidrop ID Number ACK Acknowledgement character 13 3 ModBus Communications Protocol e BioFlo1
160. ule in liquid of any kind 8 1 Cleaning the Modules Use a sponge dampened with mild detergent and water to wipe the exterior of each BioFlo 110 module Rinse the sponge in clean water then wipe the exterior again 8 2 pH Probe Maintenance and Storage The probe should be stored standing upright with the electrode tip immersed in a solution of 3 molar KCl or a buffer solution between 4 00 and pH 7 00 IN CAUTION Never let a pH probe rest on its tip Never leave a pH probe in DI water 8 3 dO2 Probe Maintenance and Storage Use soft facial tissue to clean the dO2 probe Check the probe s Teflon membrane to be sure there are no punctures puckers or wrinkles If there are the probe should be replaced BioFlo 110 Modular Fermentor M1273 0054 User s Guide 115 When it is not in use in the vessel 4 2 probe should be stored standing upright with the shorting cap in place and the membrane isolated from the air environment At no time should the probe be allowed to rest on its membrane IN CAUTION Never let a dissolved oxygen probe rest on its tip 8 4 Cleaning the Vessels After each use the vessel and the vessel components should be thoroughly cleaned If necessary decontaminate the vessel by autoclaving If you are autoclaving your vessel fill it with water to the maximum working volume This will allow for easier clean up later IN CAUTION Never allow the drive assembly to rest on the imp
161. ust the display s Y axis use the Disp Hi and Disp Lo selections as explained in Section 7 4 3 6 Finally set Control to Auto to turn on the gas flow You will hear the solenoid valve click and gas will come through the sparger 7 It is important to set the O2 Mix and 02 before turning the Gas loop control on to avoid a sudden burst of large bubbles into the vessel 7 Press the X key to return to the Gas loop screen The Demand and 4 Gas options work as explained in Sections 7 11 1 and 7 11 2 The presence of the does not affect these controls 14 3 4 Cell Culture Mode Cascades Before you set cascades first review the table at the top of Section 14 3 to identify the cascades available according to the combination of modules in your system If you have the TMFC without the Gas Mix Module the dO2 Cascade screen will look like this see sample screen on following page New Brunswick Scientific User s Guide 152 14 4 J 402 73 1 E Cascade To None Agit 5 Agit Hi Limit 150 Gas Agit Lo Limit 25 1 Gas Hi Limit 5 0 Agit Gas 1 Gas Lo Limit 0 0 None 0 P Gain 0 05 0 25 If you have with the Gas Mix Module dO2 Cascade screen will look like this d dO2 73 1 Cascade To None 4 Gas Agit Hi Limit 150 Demand Agit Lo Limit 25 Agit None Advanced Control P Gain 0 05 l Gain 0 25 Make your choices as explained in Sec
162. ver Assembly 135 Fuse Holder Power Inlet 135 Fusing Arrangement 136 Gas Mix Controller Rear 8 Harvest System 31 50 Index of 162 Level Controller Front 61 Level Controller Rear 8 Motor Installation 55 Non Jacketed Vessel 17 PCU Touchpad 85 PCU Front 84 PCU Rear 5 Power Controller Front Panel 59 Power Controller Rear Panel 6 Pump Module Rear 9 Sample BioFlo 110 Installation 11 Sampler System 31 50 51 Stacked Modules amp Vessel 11 TMEC Front 145 Vessel Autoclave Rack 78 Vessel Bumper Installation 21 Voltage Selection 133 Water Solenoid Valve 48 Water Jacketed Vessel Assembly 36 Drive Assembly Handling 15 E Essential Warnings 14 Exhaust Condenser Part Numbers 120 F Filling the Water Jacket 76 Foam Control 112 Foam Exhaust Tube Installing the 25 44 Foam Probe Installing the 24 43 Foam Trap Kit Part Numbers 120 Fuse Replacement 117 Fuses Part Numbers 120 BioFlo 110 Modular Fermentor M1273 0054 G Gas Mix Control Modes for 106 Setting the 106 H Handling Tips 15 Harvest Tube Installation of 24 43 Headplate Arrangement of 164 Installation of 34 54 Headplate Clamping Ring Installation of 40 Headplate O Ring Replacement 117 Headplate Port Fittings Part Numbers 120 Headplates 1 3L 18 37 3 0L 19 38 7 5L amp 14 0L 39 Heat Blanket 16 Installation of 20 Heat Blanket Connection 60 Heater Connection 60 How to set the pumps 109 I
163. wick Scientific User s Guide 94 B a pH o Output 100 0 Control 4 Setpoint lt Disp Hi 1 1 0 Z Calibrate lt Setup lt The procedures to set the Control Mode the Setpoint and the Graphic Display High and Low parameters are the same as for the Temperature and Agitation Loops 79 NOTE Be sure to calibrate the pH probe following the procedure outlined Section 4 13 before autoclaving it with the vessel 7 6 1 Setting a Deadband for pH control deadband is a tight plus and minus tolerance around the setpoint This is a useful feature for example to trigger an alarm via supervisory software 1 In the pH Loop Details screen press the Setup selector button The pH Setup screen opens pH 6 53 pH Defaul Deadband 0 00 Deadbar d Output Multiplier 100 00 Multip ier Select Defaults at any time during a Setup process to return to the factory set default settings Advanced Control P Gain 140 00 P Gain l Gain 14 00 ion Gain QOO 2 Press the Deadband selector button A highlighted text box opens BioFlo 110 Modular Fermentor M1273 0054 User s Guide 95 6 53 Defaults Output Multiplier 100 00 Multiplier Advanced Control P Gain 140 00 P Gain 14 00 Gain 3 Using the touchpad enter the desired deadband value
164. wish to return to the default P amp I Gain settings open the dO2 Loop setup screen and press the Defaults selector button See Section 12 5 2 for the dO2 P amp I control equation New Brunswick Scientific User s Guide 106 7 10 Setting the Gas Mix for Fermentation If you are using Gas Mix Controller your system s Main Screen will include an oxygen O2 loop From the Main Screen of the appropriate fermentor press the selector button in the O2 row to open the O2 loop screen 0 0 Control Off Control ontrol ontro E Output 0 0 Se Setpoint lt ap Disp Lo aa 1 1 Press the Control selector button 2 In the next screen press the Manual selector button 3 To cascade O2 to your dO2 loop see Section 7 8 2 4 Toset a manual O2 percentage without cascading press the Setpoint selector button in the O2 loop screen see sample screen above and enter the percentage of oxygen needed The controller will automatically feed oxygen as needed to maintain the setpoint you have entered 7 11 Setting the Gas Mix for Cell Culture If you are using Gas Mix Controller your system s Main Screen will include a Gas loop From the Main Screen of the appropriate fermentor press the selector button in the Gas row to open the Gas Mix Screen Gas Mix for this controller is not actually a mixing process it is an automatically sequenced feeding of various gases Four control mode
165. y for the 8th loop opens the Gas Loop Details screen where you can set the Control mode to Auto or Off enter Setpoint 0 20 SLPM in Fermentation mode or 0 5 SLPM in Cell Culture mode amp set Hi and Lo display limits The dO2 Setup screen shows Gas Flow cascade Hi and Lo limits in addition to the above information Press the appropriate selector keys to change these limits Pressing the Cascade key in the dO2 Setup screen reveals dO2 cascade selections of Agitation Agit Gas Flow Gas Agitation Gas Flow Agit Gas or None BioFlo 110 Modular Fermentor M1273 0054 continued User s Guide 147 Module s Present Gas Mix TMFC 8th Loop Operation YES YES Gas In Fermentation mode the system is configured for gas flow control with oxygen supplementation Press the Gas loop selector key to open the Gas Control screen where you can use the selector keys to set these parameters for gas flow Control mode Auto or Off Setpoint 0 20 SLPM oxygen enrichment O2 Mix Control mode and output percentage 2 The dO2 Setup screen shows Gas Flow cascade Hi and Lo limits in addition to the above information Press the appropriate selector keys to change these limits Pressing the Cascade key in the dO2 Setup screen reveals dO2 cascade selections of Agitation Agit Gas Flow Gas Gas Flow Oxygen Enrichment Gas O2 Agitation Gas Flow Agit Gas Agi

Hot Link to New Brunswick BioFlo 110 Operations Manual

Contents

Download Pdf Manuals

Related Search

Related Contents