ArtGene TM ThermalCycler A100/A200
Contents
1. 26 1 Introduce the System Setting Screen 27 sy a AA PAA ee Sees eae Su EE 28 J INCUDANION 22 0 APA AA Waska 28 4 Gradient Calculator x essere ceeds BAET BAD BALAK DE RES DAANG RP EE 28 Ds VIEW REDON bac chad chee eed bee ke ne Pe ewe eee ee oe bed bee ee wd 29 Oe Lor C IOO Dr ius ador RESQUE be ee wuss gusa sassa be kan es ass aa eee EE 29 ARE e PP PP AA 30 OQ Congre SOLO zd aaa aka ER RIA UIT EROR AERE ER ahus oas HG AG IS 30 SOMO DEOBIS 5 a u U Uu Tl doa rod brodo a S enel we ee dit 31 9 I Access CODIFOL 2 22 u oes pa pusqauqgupia gaa aguas iha RS ass RS RR ES 31 9 2 Thermal Cycler Name IP 32 0 3 Sel Dale Teu ix casey Yn Boe E y de ESSERE PP ERE EP RIVER FEE EE 33 9 4 Run time Accuracy 1 cee RR hh 33 9 5 User Message 33 ArtGene Thermal Cycler User Manual About This Manual Please read the manual carefully before using the Thermal Cycler Purpose of This This manual provides instructions on how to set up and use A100 A200 Thermal Manual Cycler LongGene reserves the right to modify this manual at any time without prior notice iting This manual uses the following conventions iConventions Bold text indicates user operation For example Touch Ok to accept changes and return to last screen Note Provides instructions that draw your attenti2. I I lt I i last two digits of year month day hour minute l 3 From the Folder drop down list box select a folder to save the program to To create a new folder to save the program touch New Folder and enter a folder name of up to 30 characters 4 Touch Run to both save and run the program Or touch Save to save but not run the program Or touch Export to save the program to a USB flash drive ArtGene Thermal Cycler User Manual 21 D Create and Edit Program 2 Edit Program To edit a program 1 In the Home screen touch Program Library 2 In the Program Library screen select a folder and then double click it or touch View 3 In the View Program screen select a program to be edited and then and then double click it or touch View Edit 4 In the Edit Program screen you can edit the program and then save and or run it following the instructions described above 3 Program Wizard Function 22 The Program Wizard function provides a quick method to create a standard three step or two step PCR program and perform running 1 In the Home screen touch Program Wizard The Program Wizard screen opens There are two screens touch Next and Last to shift between them Program Wizard 1 Temperature Control Program Wizard 2 Denatur ation Anealing Extension ma FEN Gi i 2 In the first screen edit Temperature Control mode and Lid Temperature
3. ArtGene Thermal Cycler User Manual D Create and Edit Program Control mode You can leave these parameters as default because the default parameters can meet most PCR occasions 3 In the second screen touch and edit parameters of each step Keep parameters of a step empty to exclude the specified step You can touch Graph to view the graphical representation of the program Program Wizard 2 xX Step 2 Step 3 Step 5 Step 6 Step 7 94 0 C GoTo 72 0 C Note e Allowable temperature range 0 0 C to 99 9 C e Allowable time range 00 00 to 99 59 min sec Allowable entered cycle numbers range 0 101 The actual cycle numbers will be equal to the entered cycle numbers So if you would perform a PCR reaction with 30 cycles then you should enter a number of 30 4 Touch Save to save program or touch Run and then select whether save the program before running according the displayed message in a pop up window ArtGene Thermal Cycler User Manual 23 E Perform Run 1 Load Samples into the Instrument 1 Place PCR tubes or microplate onto the sample wells of the sample block MPORTANT Do not touch the surface of the sample block or lid surface because they may be very hot 105 C 221 F or higher Note eThe cap of tubes or film on microplate must be sealed tightly to prevent sample evaporation Tubes or microplate should have heat resistance of up to about 120 C For 0 2ml PCR tube we rec
4. This function 1s only available with A200 Thermal Cycler 1 In the Home screen touch System Setting 2 In the System Setting screen touch Gradient Calculator The Gradient Calculator screen opens 3 In the Gradient Calculator screen touch Left and Right to enter the low temperature and high temperature of the left Ist and right 12th column of the sample block The temperature range 30 0 C lt Left Rights 99 9 C The difference between the Left and Right temperature lt 30 C 4 Touch Calculate to display calculated temperature gradient from the first column to the twelfth column Gradient Calculator d Calculate E 4 5 87 7 88 0 86 9 85 8 84 5 83 2 81 8 80 5 78 1 sal B 2 3 4 5 6 7 8 9 10 11 12 K Exit J 5 After you finish touch Exit to return to the System Setting screen ArtGene Thermal Cycler User Manual 29 F System Setting 5 View Report 6 Tm Calculator 30 1 In the Home screen touch System Setting 2 In the System Setting screen touch View Report to open the screen EET E3 Run Started Run Aborted Public Admin 2011 1 14 9 53 10 2011 1 14 10 7 44 4 Public 0010 2011 1 13 16 54 2 2011 1 14 9 46 43 1 6 Public Admin 2011 1 13 16 6 38 2011 1 13 16 36 34 Public 0010 2011 1 13 15 32 30 2011 1 13 16 4 49 Public 0010 2011 1 13 15 31 23 2011 1 13 15 32 11 VW showa EN Clear J 3 Select a report and double click it o
5. f you touch Forever the program will hold forever after reaching this step until you stop it manually and you can not add any step after a forever step 4 You can also set options for a step e Increment Temp Let the instrument increment the temperature in this step with each cycle To set the Increment Temp function Touch for each cycle and then enter the number of degrees of temperature in the vr to shift between and to increase or decrease temperature textbox The allowable temperature range is 0 1 C 10 0 C Note You could not set parameters to let the temperature below 0 C or above 99 9 C after some cycles e Increment Time Let the instrument increment the holding time in this step with each cycle To set the Increment Time function Touch 7 to shift between and to increase or decrease time for each cycle and then enter the seconds in the textbox The allowable time range is 1 sec 120 sec Note You could not set parameters to let the time below zero second after a certain cycle number e Slow Ramp Let the instrument heat or cool from previous step temperature to the desired temperature for current step at a specified rate To set the Slow Ramp function Enter the value of ramp rate in the textbox Input range is 0 1 6 0 C sec Note The actual maximum ramp rate of heating and cooling varies with different instrument type and block module typ
6. Enable Guest Account c To allow login without password touch Allow Login without Password 5 Touch Ok and restart the instrument by powering off the power switch After enabling the Access Control you must login as a user on the Login screen see below before you can use the instrument O A m3 eb e Create User Guest User 2011 4 29 Fri 9 45 10 6 After you finished experiment touch p in the Home screen to logout 9 2 Thermal To enable network control function you need to set the IP address and a unique Cycler Name IP Thermal Cycler Name for each instrument When login is enabled log in as admin 1 In the Home screen touch System Setting 2 In the System Setting screen touch Admin Options 3 In the Admin Options screen touch Thermal Cycler Name IP 4 In the Thermal Cycler Name IP screen set a unique Thermal Cycler Name and IP address The name can be up to 30 characters 5 When you finish touch Ok to return to the Admin Options screen ArtGene Thermal Cycler User Manual 33 F System Setting 9 3 Set Date Time 9 4 Run time Accuracy 9 5 User Message 34 The date and time appear on the Home screen and the Run screen To set Date Time When login is enabled log in as admin 1 In the Home screen touch System Setting 2 In the System Setting screen touch Admin Options 3 In the Admin Options screen touch Date Time 4 In the Date Time screen enter today s date and current
7. Setting then Configure Shortcut The Configure Shortcut screen opens Configure Shortcut Folder Preset Touchdown1 Ps Select Program Delete Program J 2 In the Configure Shortcut screen touch a check box next to a function on the right of the screen to select a function as a shortcut 3 If you want to select a program as a shortcut touch Select Program Select Programs for Shortcut Folder Public VJ RTPCR400 2011 01 13 2011 01 13 0010 2011 01 13 2011 01 13 1 2 Admin 2011 01 12 2011 01 14 16CW 2011 01 10 2011 01 10 xc RTPCR 2011 01 09 2011 01 09 LENT 4 In the Select Programs for Shortcut screen select a folder from the Folder drop down list box and programs of the selected folder will appear Touch the header of the first or second column of the table to sort all programs Touch a program to select it and then touch OK to return to the Configure Shortcut screen The program will appear in the left side list box 5 When you finish touch Ok to return to the Home screen The selected function or program will appear in the Home screen ArtGene Thermal Cycler User Manual 31 F System Setting 9 Admin Options 9 1 Access Control 32 Note You can select no more than six functions and or programs as Shortcuts To deselect a shortcut on the Home screen you need to touch System Setting again and then touch Configure Shortcut In the Configure Shortcut screen unselect the ch
8. W USB Port Figure 1 Front View of A100 A200 Thermal Cycler Air Vent Ethernet Port RS232 Port Figure 2 Rear View of A100 A200 Thermal Cycler Table 1 Functions of A100 A200 Thermal Cycler Components Component Lid Lid Knob Lid Handle USB Port Touchscreen Air Vents Power Switch Power Inlet Ethernet Port RS232 Port Powers on 1 and powers off 0 the instrument Connects the instrument to a socket via supplied power cable Connects up to 255 Thermal Cyclers to a computer Connects the instrument to a computer rtGene Thermal Cycler User Manual A Instrument Overview 2 Menu Overview Table 2 and Figure 3 describe buttons and virtual keyboard common to many of the screens IMPORTANT Do not use any sharp object e g a pencil to touch the touchscreen in case of scratching Table 2 Buttons in the screens of A100 A200 Thermal Cycler Returns to the Home screen Closes current screen and returns to previous screen Moves between pages i e Page Up and Page Down Scrolls left or right to view graph or curve Accepts present parameters setting and closes the screen Close the screen without changing any parameters Runs a selected program or incubation mode Figure 3 Virtual keyboard in the screens of A100 A200 Thermal Cycler Texts are entered with the virtual keyboard Enter a letter A letter button Touch abc ABC to change between
9. a program are carried out via the buttons Program Screen Sets temperature control mode and lid temperature Adds a constant temperature step z Adds a gradient temperature step il Gradient Q Returns a former step and repeats additional Goto numbers SB Edits a step Edit Y Deletes a step Delete Saves the created program Ll Save Saves another copy of the opened program Runs the program 1 2 Set Temperature Control Mode and Lid Temperature 1 In the Edit Program screen touch Head The Head screen opens 2 Select temperature control mode and lid temperature control mode and then touch Ok to accept changes ArtGene Thermal Cycler User Manual 17 D Create and Edit Program 1 3 Add Step Constant Temperature Step 18 Note Because sample volume can affect heat exchange rate the actual holding time of sample kept under the setting temperature will considerable less than the setting holding time especially when the sample volume is large The Sim tube mode controls the process of heating the sample block to reach the setting temperature by considering sample volume factor while the Block mode does not consider sample volume factor When you perform PCR reaction that desires step time no more than 30 seconds for extension step we recommend you select the Sim tube mode For lid temperature control we recommend you select a temperature five to ten Celsius above
10. lowercase letters and uppercase letters Delete the last character in an Cam input field Erase all characters ArtGene Thermal Cycler User Manual 7 B Getting Started 1 Install A100 A200 Thermal Cycler 1 1 Site Requirements A100 A200 Thermal Cycler is intended for indoor use Ensure that the installation site meets conditions below Power supply Power supply for the instrument must use the supplied power cable The 3 prong plug with the power cable should be matched with a reliable grounded socket The socket should be rated at 10A 220425 VAC 50 60Hz Make sure the rated load of the socket meets the instrument specifications Universal AC 85 VAC to 265VAC which can be used in any existing power system in the world is provided by request Environmental Table 1 Temperature and humidity conditions Conditions capta rng Ambient temperature 10 to 30 C 50 to 86 F Relative humidity 20 to 8096 noncondensing Place A100 A200 Thermal Cycler on a flat table Avoid placing the instrument adjacent to heaters cooling instruments water sink or in direct sunlight Keep away from any equipment that vibrates such as a refrigerator or centrifuge The recommended distance between the instrument and the back wall is 20 cm 7 9 in Two thermal cyclers can be placed side by side 1 2 Unpack A100 A200 System 1 Remove the packaging materials and store them in case you need to ship the instrument to LongGene for servi
11. time 5 Touch Ok to accept changes and return to the Admin Options screen The Run time Accuracy function is used to adjust coefficient of estimated remaining run time when the original estimated time is not accurate The User Message function is used to manage user accounts To manage user accounts When login is enabled log in as admin 1 In the Home screen touch System Setting 2 In the System Setting screen touch Admin Options 3 In the Admin Options screen touch User Message 4 The User Message screen lists all accounts currently available Touch the table column header to sort all users ae 63 2011 04 06 2011 04 01 2011 04 01 2011 04 01 m T Create j Edit J Delete J admin control all functions in the Admin Options screen The default password for admin is admin e Guest can create rename copy modify save delete and run programs that stored 1n Public Folder Guest Folder and USB Folder All other users can create rename and delete non system folders can create rename copy modify save delete and run programs can set his or her shortcuts can modify his or her login password in the System Setting screen after login In the User Message screen a To create a new user account touch Create to open the Create User screen Enter user name and password case sensitive of no more than 30 characters b To edit user password select a user and then double click it or
12. touch Edit to open the Edit Password screen Enter new password and touch Ok c To delete a user account select a user and then touch Delete Touch Yes in the displayed pop up window to delete the user ArtGene Thermal Cycler User Manual LongGene Hangzhou LongGene Scientific Instruments Co Ltd Add C512 513 Xihu Int l Plaza No 391 Wen Er Road Hangzhou 310012 P R China Tel 86 571 8886 2165 86 571 8886 2284 86 571 8808 7653 86 571 8808 7267 Fax 86 571 8739 7572 86 571 8886 2284 818 E mail info longgene com longgenehz yahoo com cn Website www longgene com
13. with the arrow on block module pointing to another arrow on the Thermal Cycler chassis and place the block module into position in the Thermal Cycler ArtGene Thermal Cycler User Manual B Getting Started 2 Introduce the Home Screen When the instrument powers on the booting screen is displayed for several seconds After that the Home screen is displayed see below Remaining 0 9 e LastRun Program Program System Program Library Wizard Setting Ci AY Shortcut Shortcut Shortcut Shortcut About Touchd ownl 2011 6 2 Thu O a Information Bar lid temperature block temperature when the instrument is Items in the Home screen idle or when running program which set temperature control mode as Block or sample temperature when running program which set temperature control mode as Sim tube and estimated remaining run time b Shows date week and time Buttons of Home Screen When login is enabled touch this button to logout the current user Opens Run screen Go to list of the last run 10 programs LastRun Programs Go to list of folders which contain all saved programs Creates new program quickly Sets instrument parameters and shows other e PN J System Setting tools Configures shortcut for functions or programs Shows a selected program shortcut Program Shortcut Shows thermal cycler model and company information ArtGene Thermal
14. 1 Pause 7X 5 0 C Cyc 9 S Cyc 1 0 C S l Paus gt Skip J Q Stop iul Gradient AY Curve D kis GC vagin S Edit Run Started 2011 1 14 11 41 47 O v Report The items displayed on the Run screen a The colored status light the colored status light keeps green during running changes to orange after touching Pause button changes to red when error arises and changes to white after stopping running and the run status Lid lid temperature Block or Sample block temperature when the instrument is idle or when temperature control mode is Block or sample temperature when temperature control mode is Sim tube Cycle current cycle numbers total cycle numbers 11 41 53 Current Time b Program Folder name and Creator of program when login is enabled Note If you create a new program to run but did not save it the program will be given a default name Admin and will be saved in a temporary folder Temp After run the temporary program will not be recorded in the list of the Last Run Programs screen c Options of some step Pause preset pause X C Cyc Increment or decrement temperature X S Cyc Increment or decrement time X C S Ramp rate d The recent run message ArtGene Thermal Cycler User Manual 25 E Perform Run Buttons on the Run screen Pauses and Resumes run Skips run step Stops run Views bar chart of sample block te
15. ArtGene Thermal Cycler A100 A200 User Manual LongGene Contents Getting Started 1 Install A100 A200 Thermal Cycler 7 1 1 Site Requirements 7 1 2 Unpack A100 A200 System 7 1 3 Power On and Power Off 7 1 4 Open and Close Lid 8 1 5 Block Module Exchange 9 2 Introduce the Home Screen 10 Manage Folder and Program Te Manage FOEI se usuy dcbct cie lu dede KA cde Reo de ca abo Pee eke hee 11 1 1 Introduce the Program Library Screen 11 1 2 Add Folder 12 1 3 Rename Folder 12 1 4 Delete Folder ss aw vcd eas eke aw eee ee ee eK desea we dae 12 2 Manage POOL ss pagana ee ku PE EErEE Oe cansa PESE eos NG ERIS 13 2 1 Introduce the View Program Screen 13 2 2 Rename Program xam FA NG AA BAGA KG HD ee he eee ee ee ee kee ie 13 2 3 Copy Program we eee soe 14 2 4 Delete Progralfi eo oed dea RE tienoon trar ERRAT E x CRE tes dud 14 2 5 View Last Run Programs 14 Create and Edit Program 1 Create Ne
16. Cycler User Manual 11 C Manage Folder and Program 1 Manage Folder 1 1 Introduce the Program Library Screen In the Home screen touch Program Library The Program Library screen opens a Program Library xX Folder Created Date Y System Folder 2011 01 12 2011 01 12 2011 01 10 2011 01 05 2011 01 05 iQ Vis ES New j Ca Renamej ii Delete The table lists all available folders Folder names include the number of program in parentheses Touch the header of the first or second column of the table to sort all folders Touch 4 Page Up and Page Down to move between pages The Preset folder contains eleven preinstalled programs e 3Step 2000 3Step 1000 3Step 500 for amplifying less than 2kb 1kb 500bp nucleic acid templates using three step PCR e 2Step PCR two step PCR Gradient for optimizing annealing temperature of PCR reaction This program is only available with A200 Thermal Cycler Touchdownl and Touchdown2 Touchdown PCR Long PCR for amplifying of extra long nucleic acid chain RT PCR for synthesizing DNA from RNA by reverse transcriptase igation 16C for connecting segmental DNA with vector DNA at 16 C Enzyme 37C for ordinary enzyme reaction at 37 C Note Programs in the Preset folder are not allowed renamed or deleted If you want to modify a program of Preset folder you can edit the program and then save it to another folder see chapter D for details Or you can firstly c
17. ce 2 Inspect the instrument for shipping damage 3 Verify items according the packing list for example e ArtGene Thermal Cycler A100 A200 chassis and module Power cable e ArtGene Thermal Cycler A100 A200 User Manual this manual Certificate of quality MPORTANT If the A100 A200 Thermal Cycler is damaged or some items are missing or damaged contact LongGene or local distributor 4 Move A100 A200 System to the desired installation site 1 3 Power On and Power Off 1 Connect the power cable to the power inlet in the rear of the instrument 2 Connect the 3 prong plug of the power cable to the socket Make sure the power cable has connected to both power inlet and socket firmly To power on Turn the power switch to position 1 To power off Turn the power switch to position 0 Note When the Instrument is set aside for a long time disconnect the power cable from the socket SrtGene Thermal Cycler User Manual B Getting Started 1 4 Open and Close Lid The lid of A100 A200 Thermal Cycler can move down to press to reaction vessels firmly and keep the top of reaction vessels at high temperature e g 105 C to protect reaction sample from condensation I MPORTANT Temperature of the lid can be very high 105 C 221 F or higher Never touch the inner surface of the lid when the temperature is above 43 C To open the lid 1 Turn the lid knob anticlockwise see the Loosen arrow symbol o
18. e Thermal Cycler User Manual C Manage Folder and Program 2 3 Copy Program To copy a program 1 In the View Program screen select a program and then touch Copy to open the Copy Program screen 2 In the Copy Program screen select a Target Folder in the drop down list box and then touch Copy to copy the program If you want to a Rename the program before copy Touch Clear to clear the old Program Name and then enter a new name b Create a new target folder to copy the program to Touch New Folder to open the New Folder screen enter a folder name and then touch Ok to return the Copy Program screen c Touch Export to copy the program to a USB flash drive 2 4 Delete Program To delete a program 1 In the View Program screen select a program and then touch Delete A pop up window appears 2 Touch Yes in the displayed pop up window to delete the program Note Programs in the Preset folder are not allowed deleted 2 5 View Last Run Program In the Home screen touch LastRun Programs The Last Run Programs screen opens Last Run Programs Last Run Date Public 2011 1 14 10 7 53 4 Public 2011 1 14 9 53 9 1 2 FF 2011 1 13 15 31 4 Public 2011 1 13 14 16 11 Preset 2011 1 13 11 56 10 NA Rename EB con EN Clear b guy The Last Run Programs screen has the same appearance as the View Program screen but shows the recently run ten programs for quickly viewed and run again You can touc
19. e Refer to instrument specifications for the maximum value If a value larger than the block s maximum rate is entered the block s maximum rate will be used If you do not want to slow the running leave this option empty 5 Touch Ok to accept changes and close the screen ArtGene Thermal Cycler User Manual 19 D Create and Edit Program Gradient Temperature Step 1 4 Set Cycle 20 Gradient Temperature Step is only available with A200 Thermal Cycler 1 In the Edit Program screen select a step after which you want to add the gradient temperature step 2 Touch Gradient and the Gradient Step screen opens Gradient Step 3 65 5 66 0 1 Left i 0 C 63 8 61 6 59 0 56 3 3 53 7 51 0 Rightl660 ec 5 48 4 46 2 44 0 44 5 AG L Calculate i 2 3 4 5 6 7 8 9 1 1t 12 Time Hrs Min 30 Sec m Increment Time Sec m 3 Touch Left and Right to enter temperature of the left 1st and right 12th column of sample block The allowable temperature range 30 0 C lt Left lt Right lt 99 9 C Difference between the Left and Right lt 30 C 4 Touch Calculate to display calculated temperature gradient from the first column to the twelfth column 5 Touch Time to edit the holding time You can also set Pause or Increment Time as you need The allowable increment time range is 1 sec 120 sec m In the Edit Program screen select a step that you want
20. eck box for deleting a function shortcut or select the program from the program list and then touch Delete Program for deleting a program shortcut The Admin Options is used to control access mode set thermal cycler name IP address set date time set run time accuracy and manage user accounts When login is enabled only admin can see the Admin Options button amp To use Admin Options functions In the Home screen touch System Setting then Admin Options The Admin Options screen opens AN Admin Options xX e Ie IN Ka ka ka Ca Access Thermal Cycler Set Run time Control Name IP Date Time Accuracy A User Message The Access Control is used to protect personal programs and folders and limit ordinary users to modify instrument parameters To enable the Access Control When login is enabled log in as admin 1 In the Home screen touch System Setting 2 In the System Setting screen touch Admin Options 3 In the Admin Options screen touch Access Control The Access Control screen opens ArtGene Thermal Cycler User Manual F System Setting h V Require Login Allow Create New Account by Anyone Enable Guest Account Allow Login without Password WV OK __J 4 To enable the Access Control function touch Require Login a To allow anyone to create a new account in the Login screen touch Allow Create New Account by Anyone b To allow an anonymous user to login touch
21. erature incubation experiment Calculates gradient temperature of each column of the sample block Views stored run reports Calculates Tm value of a primer pair This function is reserved for service Configures functions or programs shortcuts Sets instrument parameters When a user other than admin login the Admin Options icon will change to Login Password s icon The user can edit his her login password using this function 2 About 3 Incubation 4 Gradient Calculator F System Setting To view product information about A100 A200 Thermal Cycler 1 In the Home screen touch System Setting 2 In the System Setting screen touch About and the About screen appears 3 When you finish touch Ok to return to the System Setting screen The Incubation function is used to perform a constant temperature incubation experiment such as DNA ligation or RNA digestion 1 In the Home screen touch System Setting 2 In the System Setting screen touch Incubation 3 In the Incubation screen enter the required incubation temperature If you select Lid On the lid will heat to reach 10 C above the incubation temperature by default 4 Touch StartRun to run the Incubation program and the Run screen opens 5 In the Run screen you can view block temperature by touching Gradient view temperature record curve by touching Curve and run time by touching View Timer You can stop running by touching Stop
22. h Clear to clear program list Note The Rename and Copy buttons are not available in the Last Run Programs screen ArtGene Thermal Cycler User Manual 15 D Create and Edit Program 1 Create New Program 1 1 Introduce the Edit Program Screen 1 In the Program Library select a folder and then double click it or touch View to open the View Program screen 2 In the View Program screen touch New to open the Edit Program screen Edit Program Admin Folder Public Head P Temp J II GradientJ C Goto Edik J Delete Save J Save As Pasa Note To open the Edit Program screen more quickly you can put the New Program shortcut in the Home screen see page 29 Configure Shortcut for details The Edit Program screen 1s used to create view and edit program After you add several steps the screen looks like below Edit Program 35TEP1000 Folder Preset Step 1 Step 2 Step 3 Step 5 Step 6 Step 7 94 0 C 94 0 C 0 5 0 O GoTo 72 0 C Head J A Temp J II Gradient Qe Goto P A Edit Gy Delete J 3 E Save As gt Run Items in the Edit Program screen a Step number b Temperature for a constant temperature step or highest and lowest temperature for a gradient temperature step c Step holding time hour min sec d The start step number and amounts of additional repeats for cycles MWtGenel Thermal Cycler User Manual D Create and Edit Program Buttons of Edit Setting steps of
23. mperature Views real time temperature record curve Curve D Views run time View Timer Runs program again Opens Edit Program screen to edit the program A Views run report Report View Run Timer 1 In the Run screen touch View Timer to view run time Lid 104 9 Sample 76 7 Cycle 1 8 11 41 53 Program 0010 Folder Public Time Elapsed OO 1 4 Time Remaining Ol 06 Run Started 2011 1 14 11 41 47 e Time Elapsed shows the time hour min since the run has started e Time Remaining shows the countdown remaining time hour min for the running Note The actual remaining time may slightly differ from the shown remaining time 2 Touch E3 to return to the Run screen 26 ArtGene Thermal Cycler User Manual View Bar Chart of Sample Block Temperature View Temperature Record Curve View Run Report 3 2 Control Run Pause Run Skip Run Step Stop Run Run Program Again E Perform Run 1 In the Run screen touch Gradient to view the temperature of each column of sample block in real time Lid 104 9 Sample 76 9 Cycle 1 8 Program 0010 Folder Public Step Time 0 3 36 76 8 76 8 76 8 76 8 76 9 76 9 76 9 76 9 77 0 77 0 77 0 77 0 1 2 3 4 5 6 7 8 9 10 11 12 Run pause 2011 1 14 12 5 51 Step Time the countdown holding time of the current step after the step temperature reached the setting value 2 Touch Ea to return to the Run screen 1 In the Run screen to
24. n the top surface of the lid knob to loose the lid 2 Hold lid handle to let the handle clamp close to the lid handle Lid Handle Lid Knob k lt Handle Cramp 3 Lift up the lid handle until the lid keeps opening vertically You can then place reaction vessels on the sample block or take out the reaction vessels To close the lid 1 Put reaction vessels into sample wells of the sample block 2 Hold the lid handle and handle clamp and then let the lid touch the chassis until you hear click sound The lid will be locked in place 3 Turn the lid knob clockwise see the Tighten arrow symbol on the top surface of the lid knob to reduce the lid height until you feel resistance Then continue to turn the lid knob clockwise until you hear click sound Stop turning the lid knob now and the lid will give proper force to reaction vessels ArtGene Thermal Cycler User Manual 9 B Getting Started 1 5 Block Module Exchange 10 Block module exchange can be performed within seconds without needing any tool IMPORTANT Please power off and disconnect the power cable from the socket before changing the block module To remove the block module 1 Open the lid 2 Put your hand on the left and right side of the block module and lift it up 3 Store the block module in a clean secure box To insert the block module Put your hand on the left and right side of the block module
25. ommend the AXYGEN serial For 0 5ml PCR tube we recommend the EEPENDORE thin walled 0 5ml PCR tube EPPENDORF order number 0030 124 0502 The sample wells of the sample block should keep clean The height of tubes should be the same so that the lid can keep stable and gives even pressure on each tube 2 Close the lid and turn the lid knob clockwise see the Tighten arrow symbol on the top surface of the lid knob until you hear click sound 2 Select Program to Run From the Home screen 1 To select a last run program Touch LastRun Programs select a program and then touch Run To select a program saved in a folder Touch Program Library select the desired folder and then touch View to open the View Program screen Select a program from the list and touch Run After you touch Run the Set Run Options screen opens Set Run Options Temperature control Sample volume u 10 100 2 In the Set Run Options screen edit Temperature Control and Lid Temperature Control 3 Touch StartRun to begin the run The Run screen opens 2KtGene Thermal Cycler User Manual E Perform Run 3 View Run State and Control Run 3 1 View Run State Introduce the You can view the status of run using the Run screen below Run Screen Lid 104 9 Sample 76 7 Cycle 1 8 11 41 53 Program 0010 Folder Public 4 O Step 2 Step 3 Step4 Step 5 88 0 C 0 1 55 66 0 C GoTo 66 0 C aoc
26. on or may be helpful For example Note When the instrument is set aside for a long time disconnect the power cable from the socket e IMPORTANT Provides information that must be read carefully Failure to follow the information will possibly result in damage to the instrument or the user For example I MPORTANT Turn the lid knob anticlockwise to loose the lid before opening the lid to prevent damage to the lid IMPORTANT To prevent electric shock please turn off the power and disconnect the power supply prior to cleaning SCP Technology Note This machine might have this function SCP Sample Cooling Protection is used to maintain block at 20 C while the lid is heating to the specified temperature before the actual program starting running Because the sample temperature does not increase so the activity of DNA polymerase is inhibited the likelihood of nonspecific primer binding and the yield of unwanted products such as primer dimers are decreased Suggested Operation Best Operating Angle Gesture Please refer to the best operation angle as per left figure Support For the latest services and support information go to http www longgene com 4 ArtGene Thermal Cycler User Manual A Instrument Overview 1 Instrument Component Touchscreen Air Vent Power Switch Power Inlet E gt Lid Knob Lid Lid Handle me A
27. opy the program to another folder see page 13 2 3 Copy Program for details and then modify it as an ordinary program The Public folder is another system folder and used as the default folder for saving programs Note When login is enabled all users can use programs saved in the Public folder MtGene Thermal Cycler User Manual C Manage Folder and Program When you plug a USB flash drive into the USB port a USB icon viiia appears Touch this icon to open the USB folder The USB folder contains programs that stored in the USB flash drive You can copy programs to and from the USB flash drive Buttons of Program Library Shows programs of selected folder Screen ca View Creates a new folder New Renames a selected folder S Deletes a selected folder Delete To add a folder 1 In the Program Library screen touch New 1 2 Add Folder 2 In the New Folder screen enter a folder name 3 Touch Ok to accept changes and close the screen Note The folder name is case insensitive 1 3 Rename Folder To rename a folder 1 In the Program Library screen select a folder and then touch Rename 2 In the Rename Folder screen Clear the old folder name enter a new name and then touch Ok to accept changes Note The Preset folder Public folder and USB folder are not allowed renamed 1 4 Delete Folder To delete a folder 1 In the Program Library screen select a folder and then
28. r touch Show to open the Run Report screen 4 After you finished touch E to return to the View Report screen 5 In the View Report screen you can touch Clear to clear all reports 6 After you finish touch E3 to return to the System Setting screen The Tm Calculator is used to calculate the Tm melting temperature and Ta annealing temperature for a primer pair 1 In the Home screen touch System Setting 2 In the System Setting screen touch Tm Calculator to open the screen a Tm Calculator X 50 fso nM Primer mM Salt Na Primer 1 5 Primer 2 5 t Calculate J BE epe eb la 3 Enter the salt concentration and the primer concentration 4 Enter each primer sequence and then touch Calculate to view the Tm value of each primer the average Tm value and the Ta value Note In order to develop a successful PCR reaction you may need to select a different Ta value from the value appeared on the screen 5 After you finish touch E3 to return to the System Setting screen ArtGene Thermal Cycler User Manual F System Setting 7 Check This function is reserved for service 8 Configure Shortcuts appear on the Home screen and can access various functions or Shortcut programs When login is enabled each user can configure different shortcuts To configure shortcuts 1 In the Home screen touch e Any button labeled with Shortcut Or System
29. the highest temperature of the program steps e g the pre denaturation step to protect sample from evaporation and condensation Note The lid heating will automatically turn off when block temperature below 30 C The lid will heat to the specified temperature before the actual program starting running You should not set a lid temperature below room temperature otherwise your program will never get running In A100 A200 system a PCR program comprises several steps Each new step will be inserted after the selected step while the initial step will be inserted without selecting a step The maximum amount of steps for a program is 30 1 In the Edit Program screen touch anywhere in the column for a step to select it When the step is selected the background becomes blue 2 Touch Temp and the Temp Step screen opens Temp Step 2 Options Bada mel ArtGene Thermal Cycler User Manual D Create and Edit Program 3 Edit Temperature and Time Note The allowable temperature range is from 0 0 C to 99 9 C with 0 1 C interval The allowable time range 1s from 00 00 00 to 09 06 06 hour min sec If you touch Pause the program will automatic paused as soon as the desired step temperature reach the setting value and the instrument will beep for one time Then you must touch Resume to resume the running Pause can be used when you need to remove tubes or add reagents at a step
30. to set as the last step of a cycle 2 Touch Goto and the Cycle Step screen opens 3 From the Step drop down list box select the start step number of the cycle The steps between the last step and the start step will run repeatedly for the specified cycle numbers 4 Touch Cycles to add additional cycle numbers ArtGene Thermal Cycler User Manual D Create and Edit Program Note Allowable entered cycle numbers range 1 100 The actual cycle numbers will be N 1 where N stands for the entered cycle numbers So if you would perform a PCR reaction with 30 cycles then you should enter a number of 29 5 Touch Ok to accept changes and close the screen 1 5 Edit Step 1 In the Edit Program screen select a desired step 2 Touch Edit to edit the step parameters 3 Touch Ok to accept changes and close the screen 1 6 Delete Step 1 In the Edit Program screen touch a step to select it 2 Touch Delete A pop up window appears 3 Touch Yes in the displayed pop up window to delete the step 1 7 Save Program 1 In the Edit Program screen touch Save or Save As to display the Save As Program screen Save As Program Program Name 1100418 1349 asa Clear Folder Public V NewFolder j 2 Program uses the current date and time as a default name You can enter a new name The name is case insensitive and can be up to 30 characters Note Default program name 100418 1349 AH LN
31. touch Delete A pop up window appears 2 Touch Yes in the displayed pop up window to delete the folder Note The Preset folder Public folder and USB folder are not allowed deleted IMPORTANT When you delete a folder all programs saved in the folder will be deleted at the same time ArtGene Thermal Cycler User Manual 13 C Manage Folder and Program 2 Manage Program 2 1 Introduce the View Program Screen 1 In the Home screen touch Program Library 2 In the Program Library screen select a folder and then double click it or touch View to open the View Program screen opens Touch the header of the first or second column of the table to sort all programs C Programs of Preset Created Date w Last Saved Date 2011 10 12 2011 10 12 2011 10 12 2011 10 12 2011 10 12 Buttons of View Program Screen Views and or edits program steps see chapter D for details Creates a new program see chapter D for details Renames a selected program Copies program between folders Deletes a selected program Runs a selected program see chapter E for details 2 2 Rename Program To rename a program 1 In the View Program screen select a program and then touch Rename 2 In the Rename Program screen Clear the old program name enter a new name and then touch Ok to accept changes Note The program name is case insensitive Programs in the Preset folder are not allowed renamed 14 ArtGen
32. uch Curve to view the temperature record curve 2 Touch ES to return to the Run screen 1 In the Run screen touch Report to open the Run Report screen The report contains records of run start time run stop time and manual operations during running Touch Export to export the report in BMP format to a USB flash drive 2 Touch E3 to return to the Run screen In the Run screen touch Pause and the system will beep for two times Note The temperature will continue increase or decrease until it reaches the setting temperature of a step After paused the Pause button changes to Resume button and you must touch Resume to continue the program In the Run screen touch Skip to skip the current running step and run the next step In the Run screen touch Stop and then touch Yes in the displayed pop up window In the Run screen if the running program stopped touch Run Again to run it again ArtGene Thermal Cycler User Manual 27 Introduce the System Setting Screen About Tm Calculator Buttons of System Setting Screen 2 amp tGene Thermal Cycler User Manual The System Setting screen opens AI EP NN 6 2 x 4b 9 Incubation F System Setting In the Home screen touch System Setting il E Gradient View Calculator Report C 3 u Configure Admin Shortcut Options Views product information about the A100 A200 Thermal Cycler Performs a constant temp
33. w Program cece eee eens 15 1 1Introduce the Edit Program Screen 15 1 2 Set Temperature Control Mode and Lid Temperature 16 J AUd Sle Get paa shoes iue Ohne oes 4354 BA E EE E 17 Constant Temperature Step eee ee eee 17 2 ArtGene Thermal Cycler User Manual Gradient Temperature Step 19 14 Sel Cycle TP 19 TO EOI ClO naa NG na kh EE E E E EE E T T 20 1 6 Delete Step AA AA APA 20 eee OE E E E E EE E ee eee ee 20 S cold ates APP AA AA 21 3 Program Wizard FUNCION s ca se Ka PG LAB E NAB NE NADERA qu RA ee de Qiu 2 21 1 Load Samples into the Instrument 23 2 Select Program to RUn 23 3 View Run State and Control RUn 24 3 1 View Run State succi x mra dentro re Eee ERE EX GU RE ELE RR EUR Y KEAN 24 Introduce the Run Screen ccc eee eee 24 VIEW RUN TIMO 2 ss sols TG ob Reno Ros thon Sas Sa EUER TG AERE bd 25 View Bar Chart of Sample Block Temperature 26 View Temperature Record Curve 26 View RUN RODO usauuanas te Peur pae PUE PE ENG use eS hs HA OQ NG 26 3 2 Control RUN sea ereite kana GRAE OUO TT 26 gala vPPPP P mana 26 Skip Run Step AA APA APA 26 sss oE E E PEE E EEE E E EET EE EE SZ s 26 Run Program Again
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