User Manual - White Bear Photonics
Contents
1. quartz surface to act in a hydrophilic manner which can produce poorly formed drops see Fig 3 4 b These phenomena can be exacerbated after working with high concentration protein samples due to their adherent nature The hydrophobic properties can be restored easily if the cleaning procedure is performed Section 4 1 21 Drop Technology Version 1 2 5 4 Parts that Require Re order 5 0 Appendix Part Description Part Number Replacement Plinth Package DT0600 100205 Plinth Removal Tool DT0900 200105 Fibre optic cable from TLDAcso to fibre optic coupler DT0800 300101 Reference Standard Kit containing Drop Technology RM1 Drop Technology RM2 Blank DT0600 100206 Drop Technology RM1 DT0810 300103 Drop Technology RM2 DT0810 300104 Drop Technology Blank Solution DT0810 300102 Cleaning Kit containing 2x 50ml cleaning solutions Cleaning cloths Cleaning tool DT0600 100204 Drop Technology Plinth Cleaning Solution DT0810 300112 Drop Technology Cleaning Tool DT0900 200120 Drop Technology Cleaning Cloths DT0900 200121 8mm spanner required for SMA cables DT0700 400105 Drop Technology P10 Pipette DT0820 300107 Drop Technology P10 tips Box of 96 DT0820 300108 Plinth Removal Tool DT0900 200105 Lint free tissue DT0820 300110 Detector cable from TLDAcso to Varian Cary 50 60 DT0600 1002022. Fibre Optic Coupler instrument accessory DT0810 300111 Version 1 1 Drop Technology 22 5 0 Appendix 5 5 Pipetting Technique Accurate pipetting is of critical importance in all areas of science this is also the case when using TLDA instruments What follows are a number of tips that will improve TLDA results and generally should be adopted in experiments Without accurate pipetting experiments would be non reproducible stock solutions would be inaccurate and assays would have such large errors that comparisons would be meaningless The accuracy of pipettes depends on their operator An operator needs to practice good technique and have a thorough understanding of how their pipettes work before results obtained using such pipettes can be described as accurate e Understanding how a pipette works More than likely in the vast majority of applications in a modern laboratory an air displacement pipette is used An air displacement pipette is a bit like a syringe except that there is an air filled cushion between the piston and the sample The air cushion prevents the piston from coming into contact with the solutions which is good but it also puts some limitations on the pipette The volume of the air cushion is affected by temperature and pressure and volatile solvents can evaporate into it Each of these affects pipetting accuracy The barrel of an air displacement pipette is also vulnerable to contamination by t
3. Notices 2 0 Installation we Te 2 1 Preparing the Cary 50 60 ccccsccsscsscessesscsscsscssccsscsscescesccsecsscsscesseseessesessseases 7 22 TED Aco ASSEMDIY na cad iakend asec tense eeleoes SATU RNA es ass Ta 2 3 Connecting the TLDAcsoto the Cary 50 60 ccccccsscsscescescesccsscsscsscesseseessesssesees 8 2 4 Computer Requirements ccccccessccsssseceesececssseccesaeecseseecesseeeseseeeeeseeeeeseeeseaeees 9 2 5 SoftWare Mstalati hes enering a E A een ee A Res 9 3 0 General Operation ccccccesssccccccceccccececeeccesseseeeessessstaeees 10 3 1 TLDAcso Functionality c ccc cesccesscesscesscessecescceseeeseceseccesecesecesecessseesenseeeseceseeees 10 3 2 SaMpl e Eva poration eiea e a S EEE ARRETE AIN 10 3 3 Takifg a measurement araye nnair aTr E KE 11 AO Maintenance seirene EEE EE A E 14 4 1 Cleaning the Plinth ccc cccccsccsccssccsseceseceseceseessecesscesecesecessscssecsseeeseeeseeeseees 14 4 2 Method of Cleaning the PHinth cccccscesscesscessecesecesecesscesscssecsseceseeeseeeneees 14 A 3 Replacing the Plinth esep ieis REEERE A 15 4 4 Method of Replacing the Plinth cccceessccsscessscsecesecesecesecsseceseeeseeeseeeneees 16 SO ADHERO oirr e E EA EAE 5 1 Solvent Compatibility 5 2 TLDAcsoSpecifications 5 3 Troubleshooting 5 4 Parts that Require Re ordef cccccscccssscsscessccesecesecesecesseceseceseceseseseeeseeeseeesee
4. the tip but they can be a good option in some cases A cheaper alternative is to use and air displacement pipette with barrier tips however these only address some of the issues The below picture shows 2 positive displacement tips plungers Practice Good Pipetting Technique Know how to pipette properly Pipette with a slow smooth action Hold the pipette vertically when drawing liquid in Only immerse the tip slightly when drawing liquid in otherwise the outside of the tip will be coated with liquid which will be transferred along with the volume inside the pipette When dispensing the liquid hold the pipette vertically but keep the sidewall of the receiving vessel at 45 degrees Pipette against the sidewall or into the liquid that s already there e End users should check the accuracy of pipetting by dispensing 100uls onto a fine balance The mass of the droplet you make should be around 0 1 g Now do the same thing 10 times and record the masses obtained If the variation is more than 0 5 the pipette may need to be looked at or more practice is required e Pre wet the tip When dispensing liquid from the pipette a coating of the sample is left on the tip making the expelled volume slightly less than it should be Pre wetting the tip before pipetting will help this Just draw up the liquid into the pipette then dispense it back into the original vessel The coating is now on the tip so when liquid is drawn up
5. were previously placed Screw into place and secure using the integral locating screw on the base platform of the coupler Fig 2 4 A STEP 5 Screw the other end of the fibre optic cable onto the fixture on the top of the fibre optic coupler accessory Once finger tight use the 8mm spanner to tighten the connection by another 30 Fig 2 4 B O STEP6 Connect the Data Cable from the back of the TLDAcso to inside the Cary 50 60 Red Arrow TLDAcso data cable connected Blue Arrow Internal Cary 50 60 detector cable disconnected If assembly is carried out correctly the apparatus should be set up as shown in Fig 2 5 Version 1 1 Drop Technology 8 2 0 Installation e The Cary 50 60 Win UV software which is supplied with the Cary 50 60 is sufficient to perform all spectral data capture and or manipulation when used in conjunction with the TLDAcso e Microsoft Excel can be used to manipulate any numerical data attained from results rer NOTE The Cary 50 60 Win UV software must be installed and in use before the TLDAcso is connected e Once the Cary 50 60 is powered on the TLDAcsg is ready for use 9 Drop Technology Version 1 2 3 0 General Operation e The top of the TLDAcso body can be rotated by 120 to reveal the sample chamber Clockwise or counter clockwise rotation will open left handed or right handed sample chambers to aid both left and right handed us
6. Drop Technology Its the light inside Transmitted Light Drop Analyser TLDAcso User Manual Version 1 2 Drop Science a technology The Decisive Step Towards the Future tiny drops yield oceans of data www droptechnology com Drop Technology It s the light inside www droptechnology com Thank you for purchasing the Drop Technology TLDAcso Your instrument has been manufactured with the utmost care and has been tested prior to dispatch For technical support please contact your local representative or go to www droptechnology com Please ensure that you know your instrument model and individual serial number before you get in contact NOTE It is strongly recommended that you read this manual fully prior to using your instrument REGISTRATION Please register your product Drop Technology periodically publishes information relating to this product We can alert you of these updates if you are on our user list All information supplied to Drop Technology is completely confidential You can register at www droptechnology com Drop Technology Ltd Tallaght Business Park Whitestown Dublin 24 Ireland Tel 353 1 4523293 Fax 353 1 4523967 Email info droptechnology com www droptechnology com CONTENTS LO JAtHOGDUCCION od r AT eecdee EAA 1 1 1 About the TEDA esane e e EA E E E E E AONE 1 1 2 Index of Parts occ cceeceseceteeeeee 3 1 3 TLDAcso Inspection and Environment 1 4 Safety
7. TON If the plinth is fitted correctly this attachment should lift off easily revealing the new plinth underneath Fig 4 13 Fig 4 14 STEP 8 Place the lid of the TLDAcso back on top of the body Fig 4 14 Fig 4 15 STEP 9 Screw the centre screw back fully Fig 4 15 Screw the fibre back into the SMA connector Fig 4 16 and tighten another 30 with the spanner provided Fig 4 16 STEP 10 The TLDAcso is now ready for use Version 1 1 Drop Technology 18 5 0 Appendix The TLDAcso is compatible with most solvents used in a laboratory Solvent Material Acetic Acid dilute Acetone Benzene Butanol Carbon Tetrachloride Chloroform DMF Dimethyl Formamide Ethanol Ether HCI dilute Hexane lsopropanol Methanol Sodium Hydroxide dilute THF Tetrahydrofuran Toluene Quartz Plinth DDDWDDDDDADADAADAADADADAD Acetal Plinth Housing R Resistant LR Limited Resistant NR Non Resistant 19 Drop Technology Version 1 2 5 0 Appendix INSTRUMENT TYPE TLDAcso SAMPLE SIZE 1p to 4ul PATHLENGTH Dependent on sample size Pathlength determined from mathematical physical model and verified experimentally LIGHT SOURCE Pulsed Xenon DETECTOR TYPE Silicon Photodiode WAVELENGTH RANGE 190 1100 nm WAVELENGTH ACCURACY 0 5nm SPECTRAL RESOLUTION 1 5 nm ABSORBANCE ACCURACY lt 2 DETECTION LIMITS 4 0 ng ul dsDNA 0 2mg
8. again and dispensed into the receiving vessel none of it will be lost to wetting This is only recommended for volumes greater than 10uls Maintain inspect and store pipettes properly e Have pipettes serviced every 6 12 months or more frequently depending on the accuracy required The service should include re calibration greasing of the moving parts and replacement of any worn out seals or other parts It s best to have this done by an experienced pipette engineer Pipettes should be wiped down with 70 ethanol before they are used every day Pipettes should be disassembled and cleaned internally and externally with 70 ethanol on a weekly basis Version 1 1 Drop Technology 24 5 0 Appendix e Inspect pipettes daily for damage to the nose of the barrel where the tip is fitted or any other obvious damage If there is an issue have it serviced because it is unlikely to be fit for purpose e Store pipettes vertically using a pipette holder This prevents any liquids that have sneaked into the barrel of the pipettes from getting any further inside and corroding them Use the correct technique for the sample type being pipetted See forward and reverse techniques outlined below Forward Pipetting Press the operating button to the first stop Dip the tip into the solution just under the liquid surface to a depth of 2 3mm and slowly release the operating button Wait 1 2 seconds and withdraw the tip from the liquid touching
9. against the edge of the reservoir to remove excess liquid Dispense the liquid onto the plinth by gently pressing the operating button to the first stop and then after a short delay press the operating button to the second stop This action blow out will empty the tip Release the operating button to the ready position Ready Position Suitable for Standard liquids aqueous and nucleotide solutions Genomic DNA amp PCR products Reverse Pipetting Press the operating button to the Second stop Dip the tip into the solution just under the liquid surface 2 3mm and slowly release the operating button Wait 1 2 seconds and withdraw the tip from the liquid touching against the edge of the reservoir to remove excess liquid Dispense the liquid onto the plinth by gently pressing the operating button to the first stop This volume is equal to the set volume Hold the button in this position The liquid that remains in the tip should not be included in the delivery The remaining liquid should now be discarded with the tip or delivered back into the reservoir Release the operating button to the ready position Suitable for High viscosity Ready Position 1 2 3 4 or foaming liquids protein and highly First Stop concentrated nucleic acid solutions Second Stop Pipette slowly to avoid bubble formation 25 Drop Technology Version 1 2 5 0 Appendix Never e Put pipettes on t
10. ens 22 5 5 Pipetting Technique ccccccsccsscessccssccssecsseceseceseeesecessecesecesecesseeeeeeseeeseceseeens 23 5 6 Warranty cies aaa reia E TA AIEEE asad oec ds ocd duet Nal Savbcsevec desea bees 26 1 0 Introduction The TLDAcsoe is a simple to use UV Visible instrument based on the physics of drops which leads naturally to more reliable instruments e The abbreviation TLDA stands for Transmitted Light Drop Analyser The TLDA instrument can be purchased as either an accessory as is the case with the TLDAcso and the TLDAocean Or as a complete integral laboratory instrument which is the case with the TLDA ve e In traditional UV Visible spectrophotometers a sample is placed within a cuvette whereas within the TLDA instrument the cuvette is replaced with a microvolume drop sample 1 4ul e It relies upon the TLDA patented technology This is based onthe physics of surface tension which holds the microvolume drop sample in place The sample is in the form of a sessile drop that in the TLDAcso adheres very strongly to the edge of the hydrophilic plinth Importantly the drop shape is virtually constant regardless of the liquid under test because of the dominance of this surface tension force over the gravitational force The gravitational force is symmetric and therefore so is the micro drop about its vertical axis e Upon closing the lid of the TLDAcso the light source and spectrometer are ali
11. ere is stable environmental conditions The ambient temperature should be between 10 C and 35 C and the humidity should be between 8 and 80 non condensing 5 Drop Technology Version 1 2 1 0 Introduction e If the Drop Technology TLDAcso accessory has just been unpacked or has been stored in a cold environment it should be allowed to come to thermal equilibrium for 2 3 hours in the laboratory before use This will prevent the possibility of failure as a result of internal condensation e Contact your supplier immediately if you experience any unexpected difficulties with the Drop Technology TLDAcso CAUTION NEVER LOOK DIRECTLY INTO THE BEAM OF UV VIS LIGHT FROM THE LIGHT SOURCE It could cause permanent or temporary blindness ENSURE THAT THE LAMP IS SWITCHED OFF PRIOR TO CONNECTING AND DISCONNECTING THE FIBRE OPTIC CABLE This will avoid eye damage caused from exposure to the light source ta NOTE BEFORE MEASURING SAMPLES ALLOW THE LAMP IN THE CARY 50 60 SPECTROPHOTOMETER TO WARM UP This takes 30 minutes as recommended by the manufacturer NOTE If the solutions to be tested with the TLDAcso instrument are flammable corrosive toxic or otherwise harmful all due care and attention must be practiced while working with such substances to minimise risk to the operator and the instrument i e good laboratory practice and consultation with the Solvent Compatibility Table i
12. ers e The size of the drop is defined by the user The Drop Technology Pipette must be set to the required sample volume 1 4 uL Drop Technology recommends an optimum drop size of between 2uL and 3uL e Pathlength is dependant upon drop size The Pathlength volume relationship is determined by a mathematical physical model of the TLDAcso system e The Drop Technology 10 uL pipette is recommended for use with all TLDA instruments NOTE Evaporation of the sample may become an issue with the use of certain samples If the samples have a high vapour pressure or a long timeframe is used measures must be taken to ensure that they do not evaporate during the reading as this would have a detrimental effect on the result e The TLDAcso design incorporates a circular channel highlighted in red below in the plinth enclosure that can be filled with liquid to create a saturated atmosphere within the closed testing chamber This evaporation well can be filled with the sample liquid or deionised water depending on the requirements Fig 3 1 Saturation channel highlighted on plinth e The desired liquid once pipetted into the well and left to equilibrate will act to slow down the evaporation rate within the chamber and lead to better results Version 1 1 Drop Technology 10 3 0 General Operation 3 3 Taking a measurement NOTE Before taking any measurements make sure that you have your blanking solution sample solu
13. gned so that the light path between them is vertical The light is focussed as it passes through the sample drop on the plinth The pathlength of the drop is directly related to the volume of the drop selected e The design of the TLDAcso eliminates the need for cuvettes both standard size and low volume cuvettes as in conventional spectrophotometers The cleaning of cuvettes is time consuming and can introduce errors The TLDAcso allows for rapid clean up with no measurable sample carryover 1 Drop Technology Version 1 2 1 0 Introduction e Due to the small sample size it has the capability to measure highly concentrated or highly absorbing samples without dilution e The TLDAcso system is designed for a wide range of applications from nucleic acids and protein quantification to any general UV Visible measurements e The Drop Technology TLDAcso unit is an accessory instrument designed to be used in conjunction with an Agilent Varian Cary 50 60 spectrophotometer enabling a full UV Vis spectrum 190 1100nm e The Drop Technology accessory enables the user to measure microlitre drop samples lt 4ul with a high degree of accuracy and precision e The Cary 50 60 pulsed Xenon lamp provides the light source and a remote detector is used to analyse the light after it has passed through the sample e Spectral information is generated using the Cary 50 60 WinUV software e The TLDAcso is ideally suited to applications where sam
14. he pipetted solution This can be an issue if you are working with corrosive or bio hazardous materials The drawings below show how an air displacement pipette works a b c d a The piston moves to the appropriate position when the required volume is set b The button is pressed to the first stop prior to sample aspiration The piston descends and expels a volume of air equal to that indicated on the volume setting c After immersing the tip into the liquid the button is released This creates a partial vacuum inside the tip and the pressure forces the volume of liquid into the tip d To dispense the sample the button is pressed to the first stop again The air pressure increases inside the shaft and the tip The compressed air pushes the liquid out of the tip To empty the tip completely the button is pressed to the second stop blow out e Consider another pipette type depending on your application The information in this section relates to air displacement pipettes but in certain situations a positive displacement pipette may be a better option Positive displacement pipettes also 23 Drop Technology Version 1 2 5 0 Appendix work like a syringe but unlike air displacement pipettes they don t have an air cushion This makes them more accurate for pipetting volatile solvents and more suitable for pipetting corrosives and bio hazardous material They are expensive because the barrel is replaced as part of
15. he plinth Fig 3 5 NOTE Do not rotate the lid more that 120 at one time Always allow for the lid to click into place before moving it again STEP 5 Use the Cary 50 60 Win UV software to take a measurement of the blank sample Version 1 1 Drop Technology 12 3 0 General Operation Fig 3 6 Fig 3 7 STEP 6 Once the blank is completed open the sample chamber If desired the sample can be retrieved using the Drop Technology Pipette Alternatively the sample area can be cleaned using lint free absorbent wipes Fig 3 6 These or similar wipes are recommended by Drop Technology for their highly absorbent and their lint free properties This simple wiping with a lint free tissue should be all that is required to sufficiently minimise sample carryover between successive measurements STEP 7 Pipette the drop of sample solution onto the raised centre of the quartz plinth Fig 3 7 Refer to Fig 3 4a and Fig 3 4b to ensure that the drop pipetted is suitable STEP 8 The procedure is then repeated for the subsequent sample drops The absorbance values will be displayed at the selected wavelengths 13 Drop Technology Version 1 2 4 0 Maintenance e Though an absorbent lint free tissue used between samples removes sample carry over decontamination of the plinth surface should be carried out regularly e If a sample is i high in concentration ii biological iii a protein or i
16. heir side with liquid inside the tip The liquid might get into the pipette barrel and cause some serious corrosion damage e Set the dial past the stated upper limit of the pipette on variable volume pipettes as this could offset the calibration e Use more pressure than is needed on the plunger as this could damage the piston over time Always e Take the ambient temperature into account Pipettes are calibrated at room temperature When working at a different temperature e g in a cold room pipettes will not dispense the displayed volumes e Take the sample temperature into account In a recent Nature Methods publication Millet and Barthlen observed a strange phenomenon where when repeatedly pipetting cold samples the first dispensed volume is always larger than expected but subsequent pipetting with the same tip gave the correct volume The reverse was true for hot samples the first dispensed volume was smaller than expected Their solution was simple dispense the first volume back into the original vessel then start pipetting e Use a sensible pipette for the volume to be dispensed The accuracy of a pipette decreases as the dispensed volume approaches the minimum volume of the pipette So for dispensing 15uls for example a 1mL pipette would be terrible a 200ul pipette not so good and 20ul pipette ideal We recommend the Drop Technology P10 pipette for use with TLDA instruments as the instrument has been optimised for use w
17. ith this pipette e Use well fitting tips Poorly fitting tips allow air to escape when drawing up and dispensing leading to inaccurate results 5 6 Warranty All TLDAocean Spectrophotometers and accessories manufactured by Drop Technology are warranted against manufacturing defects in parts and labour for a period of one year This is a return to base Warranty and a proof of date of purchase should be provided with returned goods Please contact Drop Technology or the distributor before you return an item Version 1 1 Drop Technology 26 Drop Technology It s the light inside Drop Technology Ltd Tallaght Business Park Whitestown Dublin 24 Ireland Ph 353 0 1 452 3297 Fax 353 0 1 452 3967 info droptechnology com www droptechnology com
18. ml BSA MAXIMUM CONCENTRATION 600 ng l dsDNA 20mg ml BSA TYPICAL REPRODUCIBILITY 8 mg ml BSA SD 0 4mg ml CV 4 9 96 REPLICATES 400 ng ul DNA SD 10ng ul CV 3 9 MEASUREMENT SAMPLE LOADING AND CLEANING TIME lt 10 seconds DIMENSIONS 150 X 185mm D X H WEIGHT 2kg SAMPLE PEDESTAL MATERIAL Quartz OPERATING VOLTAGE UNIVERSAL INPUT 100 TO 220 VAC 50 60 HZ OUTPUT 12VDC 19VDC 3AMP 3 5AMP MAX FIBRE OPTIC CONNECTION TYPE SMA 905 Version 1 1 Drop Technology 20 5 0 Appendix Air bubble interference If air bubbles are contained within the sample or blank drop during measurement this will cause the light to scatter and will adversely affect the result Particular attention should be paid to samples containing high protein concentrations these samples are prone to the formation of micro bubbles Detergents are also noted to cause bubble foam formation If high concentration protein measurements or samples containing detergent are to be analysed replicate readings are recommended to offset and identify anomalous results due to these phenomena Hydrophobic sample surface The quartz sample loading surface on the plinth is designed to be hydrophobic so that the sample drop sits up proud from the quartz surface and forms a sessile drop prior to measurement Due to carryover adherence or drying of the sample on the quartz surface this property of the loading surface can be temporarily lost This leads the
19. n Section 5 1 If the MSDS for dangerous samples can be attained it is recommended that it is read and fully understood prior to using the sample e Ifthe accessory is used in a manner not specified or in environmental conditions not appropriate for its safe operation the protection provided may be impaired and accessory warranty may be withdrawn Version 1 2 Drop Technology 6 2 0 Installation NOTE The following two actions must be completed before the Cary 50 60 can be synchronized with the TLDAcso The Cary 50 60 has an internal detector that needs to be unplugged before the TLDAcso is connected This connection is on the side wall at the rear of the sample compartment NOTE Remove any sample cuvette holders or accessories that are inside the Cary 50 60 STEP 1 Screw one end of the fibre optic cable onto the SMA905 connector on the lid of the TLDAcso Once finger tight use the 8mm spanner to tighten another 30 Fig 2 1 NOTE The fibre must not be Te loose Fig 2 2 STEP 2 Using a screwdriver unscrew the clamp at the rear of the TLDAcso Fig 2 2 7 Drop Technology Version 1 2 2 0 Installation Fig 2 3 STEP 3 Fit the clamp around the fibre optic cable and screw it back into place Fig 2 3 This retention clamp is designed to minimise any risk of damage to the fibre Fig 2 4 STEP 4 Place the fibre optic coupler inside the Cary 50 60 where the sample holders
20. nged once a year e t may be the case that there is an evident scratch on the surface of the plinth or that an extremely corrosive sample has damaged the quartz surface finish In this instance it is up to the user s discretion to replace the plinth as required 15 Drop Technology Version 1 2 4 0 Maintenance STEP 1 NOTE Laboratory gloves must be worn at this point Unscrew the SMA connector that connects via fibre optic cable from the light source to the lid of the TLDAcso Fig 4 5 Fig 4 6 STEP 2 Unscrew the centre screw fully and remove Fig 4 6 Fig 4 7 STEP 3 Lift off the lid of the TLDAcso to reveal the plinth underneath Fig 4 7 STEP 4 Using the tool provided Fig 4 8 Version 1 1 Drop Technology 16 4 0 Maintenance Fig 4 9 Place the tip of the tool under the lip of the plinth and gently lift out the old plinth Fig 4 9 Faw wW Fig 4 10 STEP 5 Open the box that contains the new plinth Fig 4 10 Try to avoid touching the quartz plinth directly Fig 4 11 Invert the new plinth and place over circular indentation on the top of the TLDAcso body Fig 4 11 Ee BROP TecRNSLosY TLDA Fig 4 12 STEP 6 Press the new plinth firmly into place and ensure that it is fitted securely Fig 4 12 17 Drop Technology Version 1 2 4 0 Maintenance Fig 4 13 STEP 7 y Lift off the attachment that came with the new plinth
21. ple is limited samples are highly concentrated or have a high absorbance and speed and analysis of the sample is important e The principal of operation of the Drop Technology TLDAcso is patent protected Version 1 2 Drop Technology 2 1 0 Introduction Fig 1 1 Fig 1 2 Fig 1 3 Fig 1 6 Fig 1 1 TLDAcsoAccessory Part Number DTO600 100207 Fig 1 2 Detector Cable that carries data from TLDAcso to Cary 50 60 spectrophotometer Part Number DTO600 100202 Fig 1 3 Fibre Optic Cable to carry light from the light source to the TLDAcso Part Number DTO800 300101 Fig 1 4 Drop Technology 10uL Pipette 0 5uL 10uL range Part Number DT0820 300107 Fig 1 5 Box of 96 Pipette Tips Part Number DT0820 300108 Fig 1 6 Fibre Optic Coupler Instrument Accessory Using fibreoptics it extends the light path of the Cary 50 60 to facilitate third party integrations It may be the case that the Cary 50 60 being used with the TLDAcso already has a fibre optic coupler in situ so this is an optional accessory Drop Technology Version 1 2 1 0 Introduction Fig 1 7 a Fig 1 7 b rok kita aital RM2 Blank Remi Fig 1 7 c Fig 1 7 Drop Technology Accessory Kit Part number DT0600 100204 Cleaning and Reference Standard kit combined shipped initially with the instrument Fig 1 7 a Drop Technology Plinth Cleaning Solu
22. tion Part Number DT0810 300112 Fig 1 7 b Drop Technology Reference standard kit including the standards Drop Technology RM1 Drop Technology RM2 Drop Technology Blank Solution Part Number DT0810 300105 can also be shipped individually See Section 5 4 Fig 1 7 c Cleaning Tool Part Number DT0900 200120 Fig 1 7 d Fig 1 7 d 8mm Spanner used to tighten the fibre connection Part Number DTO700 400105 Version 1 2 Drop Technology 4 1 0 Introduction Fig 1 7 e Fig 1 7 e Specially designed tool for Removing and replacing the Plinth Part Number DTO900 200105 Fig 1 7 f Fig 1 7 f Cleaning Cloths in an assortment of colours Part Number DTO900 200121 Fig 1 8 Fig 1 8 Lint free tissue Part Number DT0820 300110 e Before beginning any installation or operation of the TLDAcso accessory the user must ensure that all the parts are suitable for use Nothing should be visibly cracked broken or scratched Packaging and seals should be intact and unopened e Inspect the TLDAcso itself for any visible faults i e the unit should not be marked or dented If there are any manufacturer faults evident or any damage has arisen from transportation contact your supplier immediately e Remove the TLDAcso from its packaging and stand it on a rigid flat surface and check that it is fully stable in its place e The Drop Technology TLDAcso is developed for indoor use only in laboratories in which th
23. tion pipette pipette tips and lint free tissues ready Delays during the measurement process could affect results if samples have a high vapour pressure NOTE Laboratory gloves must be worn at this point Fig 3 2 STEP 1 On the TLDAcso reveal the preferred sample chamber according to your preference for left or right handed operation Fig 3 2 Fig 3 3 STEP 2 Pipette the drop of the relevant blanking solution onto the raised centre of the quartz plinth Fig 3 3 NOTE Ensure that there are no bubbles in the sample NOTE The Drop Technology pipette should be used in accordance with good pipetting technique see section 6 5 11 Drop Technology Version 1 2 3 0 General Operation Fig 3 4 a Fig 3 4 b Fig 3 5 STEP 3 The drop must be a stable sessile drop for accurate results to be obtained The drop should stand upright on the plinth be proud of the quartz surface and be of uniform shape The drop must be similar to the drop shown in Fig 3 4 a If the drop is similar to the drop shown in Fig 3 4 b It must be removed and a new sample must be pipetted NOTE If the drops are consistently like those shown in Fig 3 4 b it may be due to contamination of the plinth In this case cleaning or changing of the plinth may be necessary See section 4 0 Maintenance STEP 6 Rotate the lid back so that it clicks into place with the light source directly over t
24. v a large amount of samples are tested the plinth should be cleaned between each set of tests with the Drop Technology Cleaning Fluid The cleaning interval is best determined according to the conditions of use e It is good practice to periodically clean the sample plinth to prevent the possibility of contaminant build up The cleaning interval will depend again upon the nature of the samples being measured and the amount of use the instrument receives Fig 4 1 STEP 1 NOTE Laboratory gloves should be worn at this point Place one piece of cleaning cloth over the head of the tool Fig 4 1 STEP 2 Fix the circular ring securely over the cleaning cloth and the tool head Fig 4 2 Fig 4 3 STEP 3 Place one drop of cleaning fluid onto the plinth Fig 4 3 Version 1 1 Drop Technology 14 4 0 Maintenance Fig 4 4 STEP 4 Rub the surface gently in alternating circular movements Stop when the surface appears dry and all the fluid has been absorbed Fig 4 4 STEP 5 If a small number of samples are tested or the samples are of a low concentration deionised water provides sufficient cleaning However after cleaning with the cleaning fluid deionised water must then be used to rinse the surface When using deionised water repeat STEPS 1 4 but replace the cleaning fluid with deionised water e f the recommended cleaning procedures are followed the plinth should only need to be cha
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