Biochrom EZ Read 400 Microplate Reader User`s Manual
Contents
1. 15 C to 40 C operation Ambient temperature 25 C to 50 C storage Height over sea level operation up to 2000 m Installation Category II Voltage input 12 VDC Measurement unit Current input 4 A Power 50 VA Noise 45 dB PC connection USB A to B cable Pollution Degree 2 Voltage input rating 100 VAC to 240 VAC 50 or 60Hz Current max 1 5A 90 VAC amp full rated output load Voltage output 12 VDC Current output maximum 5A 20 to 80 operation 10 to 90 storage Power supply unit Relative humidity non condensing Spectral width at half maximum intensity Ventilation There are no special ventilation requirements Warming up time None required Wavelength accuracy 2 nm Weight kg Does not require any special lifting or carrying instructions 8 nm Biochrom EZ Read 400 User s Manual Version 1 0 10 3 8 3 9 Unpacking the EZ Read 400 Note The original packing has been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and the accessories box for re use in case of future shipments Warranty claims may be void if improper packing results in damage during transport Check the box for any visible damage during transportation In case of damage inform your supplier immediately and keep the damaged packing Place the device on a suitable working surface Remove the transportation lock foam part from2. 8 3 Troubleshooting and Frequently Asked Questions uuensensessssnnnennnnnnnnnnnnnnnnnnnnnnnnnn 25 Biochrom EZ Read 400 User s Manual Version 1 0 3 1 Declaration of Conformity for Biochrom Manufactured Products This is to certify that the following Biochrom manufactured products conform to the requirements of the following Directives EZ Read 400 Elisa 80 4001 40 EZ Read 400 Research 80 4001 41 and EZ Read 400 Flexi 80 4001 42 2006 95 EC 2004 108 EC 2002 96 EC amp 2003 108 EC WEEE 2002 95 EC Low voltage equipment safety directive EMC directive EC Directive on Waste Electrical and Electronic Equipment ROHS directive Standards to which conformity is declared where relevant are as follows EN61010 1 2010 EN61326 1 2006 EN 12100 1 2 2003 A1 2009 EN 14121 1 2007 Safety requirements for electrical equipment for measurement control and laboratory use General requirements Electrical equipment for measurement control and laboratory use EMC Requirements Safety of machinery Basic concepts general principles for design Safety of machinery Risk assessment For further information including unpacking positioning and installation of the products please refer to the user manual Signed Brian Clarkstone Technical Director Biochrom Ltd Biochrom EZ Read 400 User s Manual Version 1 0 4 2 Introduction and Intended Use 2 1 What to expect from the user
3. Ifthe serial number and type values below the button are filled with numbers of the instrument communication is successful i Click File gt Save All settings should now remain saved for future use 7 I mistakenly placed my plate in the plate transporter after started the measurement and Instrument EIER now I can t get the plate back How Be can I return the plate transporter to its original position Instrument unctions 2 Download U pload Functions Eee ___Feset iter Lead Plate Initialize STOP Base ADC Diode Values 5 St Go to Setup gt Instrument and select the Functions 1 tab Select initialize and wait a few minutes for the plate transporter to return to the home position Information Status _ Stausee Log File Biochrom EZ Read 400 User s Manual Version 1 0 27
4. good laboratory practices v Wear protective clothing when using the instrument including but not limited to laboratory gloves laboratory coat and safety glasses v Observe all cautionary information printed on the original solutions containers prior to their use v Dispose of all waste solutions safely and in accordance with the policies and practices of your facility v Operate the Biochrom Anthos Microplate Reader in accordance with the instructions outlined in this manual v Use appropriate precautions when using pathological toxic or radioactive materials Consult the MSDS of all materials used in the laboratory v Use an appropriately contained environment when using hazardous materials v Wash your hands thoroughly after handling test fluids If equipment has been in contact with hazardous substances it must be disinfected prior to shipment in accordance with the effective provisions See 6 4 Decontamination Instructions 3 3 Safety issues regarding the use of a PC with the instrument This equipment may be connected and controlled from a PC To preserve the integrity of the measuring equipment it is essential that the attached PC itself conforms to basic safety and EMC standards and is set up in accordance with the manufacturers instructions If in doubt consult the information that came with your PC In common with all computer operation the following safety precautions are advised v To reduce the chance of eye stra
5. s manual This manual will allow the user to v v Understand who should operate the instrument and why would be used Understand all the important safety information when operating the instrument How to safely unpack and install the instrument Scope of supply Technical specifications A diagram of the outside of the instrument A technical explanation of the workings of the instrument How to use the instrument and the reader control software to perform basic measurements Optional software and accessories that may be used with the instrument Helpful hints for obtaining the best measurements using the instrument 2 2 Instrument Use The Biochrom EZ Read 400 Microplate Reader is used for microplate based applications requiring endpoint or kinetic absorbance measurements from 400 750 nm in optically clear flat bottomed 96 well plates Only trained laboratory personnel should operate the Biochrom EZ Read 400 Microplate Reader The Biochrom EZ Read 400 Microplate Reader is intended for general laboratory and research use only Biochrom EZ Read 400 User s Manual Version 1 0 5 3 Safety Information This symbol indicates that a potential for safety risk Please carefully review the safety information provided below If the instrument is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired 3 1 Electrical Safety To prevent electrically related injuries and prop
6. the plate holder Powering up the EZ Read 400 Microplate Reader Connect the USB A to B cable from the PC to the instrument Connect mains lead to the instrument mains inlet Please Note Keep the area around the instrument free from clutter to allow for easy access to the mains lead Connect the mains lead to the power supply Ensure the mains lead is completely connected to the power supply Connect the power supply to the mains socket Switch on the mains socket and then switch on the power to the instrument at the back of the instrument The power indicator is located on the front left side of the unit Biochrom EZ Read 400 User s Manual Version 1 0 11 3 10 Cleaning and Disinfecting the Instrument For disinfection please follow the procedure below Only authorized trained personnel using personal protective equipment should perform the cleaning and disinfection procedures Ensure cleaning and disinfection occurs in a well ventilated room Please Note Before the reader can be returned to base for service it must be disinfected and a disinfection certificate must be completed see 7 3 Returns procedure for more details 1 Switch off the reader and disconnect it from the mains power supply and the PC 2 Carefully wipe off the entire reader with lint free tissues that have been moistened in a protein degrading mild detergent or a saline solution 3 Carefully wipe off the entire reader with non lint tissues tha
7. 2 Read Configuration Aa A re Under the Instrument tab Serial Number 3040 Type 42 r e In Baudrate select 9600 Temperature Firmware Version e v20 GEE e In COM Port select port e Type zl OK In Instrument Type select 2010 Biochrom EZ Read 400 User s Manual Version 1 0 23 7 To confirm that the instrument is connected with the computer select the Read Configuration button The serial number of the instrument should now appear in the Setup Instrument dialogue box along with compatible plate types Select File Save to save these settings i Quick Read 8 To measure a plate Go to Reading Quick or the R button in the menu bar Format Microplate v Plate Type 8x12 v Measurement Position v Plate Layout In the Quick Read dialogue box Confirm that een ul Endpoint Photometric z the correct format and plate type are selected Meas remeni niter Select All in Measurement Position to read Reference Filter the entire plate Gacel Select Endpoint Photometric for basic readings Select the measurement filter and the reference filter from the drop down menu Please Note It is important to use a reference filter to account for optical inference from the plate 9 Place plate in the plate transporter Select Start Absorbance measurements will appear in the open matrix in ADAP When prompted enter a plate ID Data can be exported to data analysis software using the Copy icon Data will paste as a m
8. DAP Basic Software and ADAP operating manual v USBAto B cable Dustcover v Power supply v Mains power cord Please Note Only use the power supply and mains power cord supplied with the instrument Liability In its original condition the instrument meets all safety regulations for safe operation If the instrument is used in a manner not specified in this user s manual safety and performance may be affected Biochrom is not liable for damages or costs caused by unauthorized alterations repairs or modifications of the equipment Biochrom EZ Read 400 User s Manual Version 1 0 19 6 Ordering Information and Accessories Table 2 Ordering Information Table 2 ORDERING INFORMATION 80 4001 40 Biochrom EZ Read 400 ELISA Microplate Reader 80 4001 41 Biochrom EZ Read 400 Research Microplate Reader 80 4001 42 Biochrom EZ Read 400 Flexi Microplate Reader B032082 ADAP Plus Software B032083 ADAP Expert Software 501087 Anthos 2010 Reader Check Plate See www biochrom co uk for a list of filters that are available for purchase Biochrom EZ Read 400 User s Manual Version 1 0 20 7 Contact Information 7 1 Table 3 Contact Information Table 3 Contact Information Department Email contact Telephone contact Sales enquiries biochrom co uk 44 0 1223 423723 Technical Support support biochrom co uk 44 0 1223 427890 Orders orders biochrom co uk 44 0 1223 427861 44 0 1223 420 164 Fax Re
9. atrix with filter wavelength with time and date Biochrom EZ Read 400 User s Manual Version 1 0 24 8 3 Troubleshooting and Frequently Asked Questions Please contact your distributor with any questions that you may have regarding the performance of your instrument Below is a list of frequently asked questions and answers 1 What is the indication range of the EZ Read 400 and how does this specification differ from the linear range The Biochrom EZ Read 400 has an indication range of 0 000 3 300 OD and a linear range of 0 100 2 500 OD We recommend that the broader indication range should be used only for relative determinations but not for determining the quantity of the absorbing material Only measurements made within the linear range should be used for quantitative calculations 2 Ifa measurement is made by the Biochrom EZ Read 400 that exceeds the maximum indication range how is that measurement represented If a well measurement at a particular wavelength is greater than 3 300 OD then the well will be represented as 9 999 in ADAP 3 When using ADAP software to control the Biochrom EZ Read 400 can specific wells be designated as blanks and can those values be automatically subtracted from the remaining wells The Biochrom EZ Read 400 comes with standard software ADAP Basic which does not allow the user to define specific wells as it is only capable of basic reader control for endpoint and kinetic measurements with no a
10. d to control the reader to perform endpoint kinetic and multiwavelength assays Data analysis options are available with a software upgrade to ADAP Plus or ADAP Expert See www biochrom co uk for more information regarding ADAP Microplate Software 1 Toturn on the instrument Connect instrument to a power source using the appropriate main lead to the power supply Turn on the instrument using the switch at the back of the instrument To connect the instrument to a PC Connect to the PC via serial port to serial port ora serial to USB port adaptor Determine the communication port com used by the instrument In the Start menu of the PC go to Control Panel System Hardware Device Manager Ports To connect instrument to ADAP software Insert the CD supplied with the instrument into PC install ADAP Open ADAP ADAP will prompt for a user ID and password Login using the pre set ID and password sadmin sadmin Once logged in specific user IDs passwords and administrative rights may be set 4 Select Setup gt Instrument in the menu bar A dialogue box will open Under the Instrument tab select Fie Inatument Functions 1 Functions 2 Dowrload Upload F Anttrumanit Postion T Baaise oo ee COM Pot come Baudrate 9600 COM Port select port as determined in step 2 or Auto Sense Instrument Type EZRead400 To confirm that the instrument is connected with the computer select the Read Configuration bu
11. eee mM Biochrom EZ Read 400 Microplate Reader User s Manual KE s SY anthos Cultek biog hrom Biochrom Ltd Cultek SLU 22 Cambridge Science Park Avda Cardenal Herrera Oria 63 Milton Rd Cambridge CB4 OFJ UK 28034 Madrid Tel 44 0 1223 423723 Tel 91 729 03 33 Fax 44 0 1223 420164 Fax 91 358 17 61 Email enquiries biochrom co uk Email cultek cultek com Biochrom EZ Read 400 User s Manual Version 1 0 1 ee Table of Contents PELE E A E T E E 2 1 Declaration of Conformity for Biochrom Manufactured Products 22222000222000000020 4 2 Introduction and Intended Use 5 2 1 What to expect from the user s manual usussesensensensnnsnenensnnnnnnnnennnnnnnnnennennesn nenn 5 2 2 AstrumentUsen a ses e iaaea E Esi aSa aaa EE 5 3 Safety informati n u a anne 6 3 4 Electrical Salate eu u en 6 3 2 Chemical and Biological Safety cccsssccecsssncececssnaececssseeeeceesseeeceessueeeeesseneeeesenes 6 3 3 Safety issues regarding the use of a PC with the instrument uuneeenneeennennneennnen 7 3 4 Safety concerns regarding moving parts cesscecesssececeesseececssaaecessseeeeseseeeeeesenaes 7 35 Instrument Inputs and Outputs na 8 3 6 Instrument Vall ARON senescence aaa EKE Eaa 10 3 7 Table 1 Rated Operating Conditions ecsscccsssseeeceessceceessaeeecsseeecesseeeeeeesaes 10 3 8 Unpacking the EZ Read 408 ae 11 3 9 Powering up the EZ Read 400 Micropla
12. erty damage properly inspect all electrical equipment prior to use and immediately report any electrical deficiencies Contact a Biochrom service representative for instrument service requiring the removal of covers or panels 3 1 1 Please note these particular electrical safety concerns v Voltages dangerous to human life are present in this device Before removing any covers or panels disconnect the power supply from the power source v Ensure that only the power supply supplied with the unit is used v The power supply may only be inserted in a socket outlet provided with a protective ground earth contact Use only the supplied mains lead cords with the instrument v The mains cord should be positioned in a way that enables disconnection in the event of an emergency v Extensions cords should not be used with the instruments v Only a qualified service engineer should adjust or repair the instrument v Use the equipment only in the intended manner 3 2 Chemical and Biological Safety Normal operation of the Biochrom EZ Read 400 Microplate Reader may involve the use of materials that are toxic flammable infectious or otherwise biologically harmful Biochrom EZ Read 400 User s Manual Version 1 0 6 This symbol denotes the potential for bio hazardous material on or around the instrument When using such materials observe the following precautions v Handle infectious samples or dangerous materials in accordance with
13. in set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings v To reduce the chance of cross contamination from biological samples use appropriate personnel protection measures and disinfectant wipes on keyboard and mouse 3 4 Safety concerns regarding moving parts Biochrom EZ Read 400 User s Manual Version 1 0 7 v Do not touch the plate or the instrument during movement of the plate transport risk of injury to the user or damage to the instrument v Keep the area around the microplate reader clear of clutter 3 5 Instrument Inputs and Outputs Note the presence of Biohazardous and General safety signs present on the instrument Review the safety information available in sections 2 1 and 2 2 i Place plate here prior to measurement Safety Symb Power Supply Input O E Figure 1 Front of the EZRead 400 Microplate Reader On Off Switch USB A to B cable Inkut Figure 2 Back of the EZREAD 400 Microplate Reader Version 1 0 8 Biochrom EZ Read 400 User s Manual Version 1 0 3 6 Instrument Validation An optional check plate see 6 Ordering Information for details can be purchased to ensure that the instrument is operating correctly Validation with a check plate does not preclude the use of controls on the plate 3 7 Table 1 Rated Operating Conditions Table 1 Rated Operating Conditions Ambient light Precaution avoid direct sunlight
14. ion All Iv Plate Layout Measurement Mode Kinetic Photometric Measurement Filter bd Kinetic Measurement Interval 50 seconds Cycles Data Reduction Biochrom EZ Read 400 User s Manual access these additional modes select Quick Read Select Kinetic Photometric from the Measurement Mode drop down menu Select Interval time seconds between successive measurements the minimum interval time is 45 seconds Select Cycles for the number of measurements that will be made in total Select Data Reduction method for analysis and Smoothing points Upper and Lower Limit Version 1 0 15 Explanation of kinetics measurement data reduction methods including smoothing points and limits are discussed in detail in the ADAP Operator s Manual Configuring the Data Reduction Parameter 4 4 Multiwavelength Measurements The Quick Read menu can also be used to perform multiwavelength measurements Up to 8 wavelengths can be selected if all 8 positions are Format Microplate ve a g installed with interference filters late Type 8x12 v Measurement Position All Vv Plate Layout Measurement Mode Multiwavelength bd Number of Wavelengths 3 v Measurement Filter 450 Select as many Measurement Filters as desired Cancel 4 5 Additional Features The Quick Read menu can also be used to measure only selected wells on the Set all wells nie plate Use the Edit menu to se
15. lect Set actual row specific rows columns for De select actual row pa measurement Specific wells can also be et actual column pea selected by highlighting the desired lected well j Desiaseeedwes MEN wells and then selecting Set Selected MEAS mens Wells If the incorrect wells were selected simply select De select all wells to start again Biochrom EZ Read 400 User s Manual Version 1 0 16 Biochrom EZ Read 400 User s Manual Version 1 0 17 4 6 Calibrating the Instrument The EZ Read 400 performs an automatic calibration of the lamp energy before it measures each well However if the user would like additional confirmation of the performance of the instrument a calibration plate containing a series of neutral density filters with known optical density can be purchased to verify reader performance See Section 6 Ordering for order details Biochrom EZ Read 400 User s Manual Version 1 0 18 5 General features 5 1 5 2 5 3 5 4 Measurement modes Single dual and multi wavelength and kinetics measurements with absorbance data output in OD on display Integrated quality control v Post sample filtering to eliminate ambient light v Automatic calibration prior to each measurement v Plate centering system positions all flat bottomed 96 well microplate conforming to ANSI SBS 1_ 2004 accurately under the optical path Scope of supply v Instrument v CD containing user s manual A
16. n many absorbance applications in the visible range Check the product data sheet for recommended reference wavelength for your specific assay 6 How can I connect the instrument to ADAP The COM port number must be 1 9 in order to work correctly If the COM port number is not 1 9 a Follow steps 1 amp 2 above b Right click on the port which the instrument is connected to and select Properties c Click on the Port Settings tab d Click on the Advanced button e Change the COM port number to anything between 1 and 9 do not use number which say in use f Click OK to save and exit back to the Device Manager window The change to COM port number may not show until you close and re open Device Manager step 1 Now that you know the COM port number and have successfully set it between 1 and 9 you need to instruct the measurement software to communicate on that COM port number To set COM port number from the software a Open ADAP default username password is sadmin sadmin b Select Setup gt Instrument wait for the program as it may try communicating at this point ignore any possible error c A window appears titled Instrument d On the right hand side set the Baud Rate to 9600 e Set the COM port number to that set in the above steps f Set the instrument type to that of your instrument Biochrom EZ Read 400 User s Manual Version 1 0 26 g Click on Read Configuration button h
17. nalytic capabilities The user can upgrade to ADAP Plus or ADAP Expert which allows many options for plate definition including designating the position of blanks controls standards CAG strips and samples as well as the definition of a method to instantly analyze the data using the defined blanks standards or controls 4 Which Microsoft Windows operating systems are ADAP compatible with All Microsoft Windows operating systems are compatible with all ADAP modules Basic Plus and Expert 5 Why dol get different readings from the same sample in different wells in the microplate Biochrom EZ Read 400 User s Manual Version 1 0 25 There are many possible sources of assay imprecision Among these the quality of the microplate is paramount to the quality of the measurements because a microplate reader sends light through a sample vertically either from top to bottom or bottom to top Use of a high quality optically clear microplate is important for reproducibility of sample measurements In addition it is important to use a reference wavelength when measuring a microplate Microplates may have smudges scratches lids and other forms of optical interference which may obstruct the light beam A reference wavelength allows for a well by well control of imperfections in the plate When choosing a reference wavelength use a wavelength at which the sample of interest does not absorb 620nm is a frequently used reference wavelength i
18. t have been moistened in ethanol or a chlorine solution 4 Soak non lint tissues that have been moistened in ethanol or a chlorine solution onto the plate transport mechanism and let it soak for 30 minutes 5 If a chlorine solution has been used carefully wipe off the entire reader with non lint tissues that have been soaked in water 6 Dry the reader by wiping it off with non lint tissues 3 11 Emergency Instructions In the event of an emergency the instrument should be disconnected from the power supply power source and PC 3 12 Safe Disposal of the Instrument This symbol can be found on the outside of the instrument It means that the Biochrom EZ Read 400 microplate reader cannot be disposed of in municipal waste The instrument must be decontaminated as detailed in part 2 8 of this section Biochrom EZ Read 400 User s Manual Version 1 0 12 3 13 Regular Maintenance The EZ Read 400 microplate reader does not require regular maintenance for successful use unless it is used in a manner that is outside of the recommendations laid out in this manual or stored in an environment that is excessively dusty or humid Please contact your distributor for any questions or concerns regarding the performance of the instrument Biochrom EZ Read 400 User s Manual Version 1 0 13 4 Software Installation and Use 4 1 Software Installation ADAP Basic is supplied with all EZ Read 400 instruments The software can be use
19. te Reader zuussensesnnnennnsnnnnnnnnnnnnnnnnennn 11 3 10 Cleaning and Disinfecting the Instrument uussessssnneessennnennnnnnnnnnnnnnnnnnnnnnnnnennnn 12 3 11 Emergency Instructions ueesseeseenssnennenenennnnennnnnnn nen iError Marcador no definido 3 12 Safe Disposal Of the Instrument i iaiia 12 3 13 BeeillarMantenanen a are enden 13 4 Software Installation and Use 14 4 1 Software Ins alone nern 14 Biochrom EZ Read 400 User s Manual Version 1 0 2 ee 4 2 Endpoint Measurementts cccccccssscccesssscccessssesctsesssesecsessesccesssoesessessseceesseseeeeses 15 4 3 Kinetic Measurements nussesseseesesnnnnnnnnennnnennnnnnnennnnnnnnnnnnnsnnennenensnnnsnsnennnnn nenn 15 4 4 Multiwavelength Measurements uussessssnsnnsesnnnnennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnsnnnnesnnn nenn 16 4 5 Additional Features nee 16 4 6 Calibratingthelnstrument an boiacisch daesaupreiubeuanecerusptemaubniesss 18 5 Genel al Fear ul GS anne 19 5 1 Measurement ride 19 5 2 Integrated quality control een 19 5 3 Sc pe Of SUDPIY anne een 19 54 EalliNV ea E AET 19 6 Ordering Information and Accessories uuuusssnnnnnssennnnnnnnnnnnnnnennnnnnnnnnnnnnnnnsnnnnnnnsssnnnn 20 7 SOWMLACE INIOTMaLION een 21 7 1 Table 3 Contact Noah 21 8 Appendiks e EE a a E a EE 22 8 1 Helpful Hints for Successful Microplate Assays ueeesenseneessesnnnennsnnnnnnnnnnnnnnnnnnnnnnnnnennn 22 8 2 Quick Start Bilde ann een een ee 23
20. tton The serial number of the instrument should now appear in the Setup gt Instrument dialogue box along with compatible plate types Select File gt Save to save settings Biochrom EZ Read 400 User s Manual Version 1 0 14 6 The instrument is now ready for use 4 2 Endpoint Measurements Quick Read 1 To measure a plate Go to Reading gt Quick or select the R button in the menu bar Format Microplate z Plate Type 8x1 2 Measurement Position All Iv Plate Layout Measurement Mode Endpoint Photometric Measurement Filter Reference Filter Cancel i In the Quick Read dialogue box Confirm that the correct format and plate type are selected Select All in Measurement Position to measure the entire plate Select Endpoint Photometric for basic measurements using a measurement and reference filter Please Note It is important to use a reference filter to account for optical inference resulting from the plate surface 2 Place plate in the plate transporter Select Start Absorbance measurements will appear in the open matrix in ADAP When prompted enter a plate ID Data can be exported to data analysis software using the Copy icon Data will paste as a matrix with filter wavelength with time and date 4 3 Kinetic Measurements The Quick Read menu is also used for other measurement modes including kinetic reads To i Quick Read Format Microplate v Plate Type 8x12 v Measurement Posit
21. turns returns biochrom co uk 44 0 1223 427861 Human Resources hr biochrom co uk Accounts accounts biochrom co uk 44 0 1223 427816 Biochrom EZ Read 400 User s Manual Version 1 0 21 8 Appendix 8 1 Helpful Hints for Successful Microplate Assays v Always insert the plate with well A1 on the upper left position v Keep plates clean measurements in microplates are made through the surface of the plate and thus the surface should be as free as possible of debris scratches and smudges Wipe the plate clean with a lint free tissue if necessary v Measurements may be affected by uneven or heterogeneous well contents Visually inspect the plate for foaming uneven meniscuses bubbles or particles in the well all of which can interfere with absorption Centrifuge the plate if necessary to obtain a homogeneous solution in the wells v Usea reference measurement for reducing measurement error caused by interference from the plate material or contamination Use a reference wavelength at which your molecule of interest does not absorb Many assays recommend a reference measurement at 620 nm v When using plate frames for column row strips or single wells breakable strips check that all strips and wells are pushed down completely and are level with the frame Keep the bottom of the plate dry If liquid has contaminated the well bottoms or if condensation is present dry the surface with a lint free tissue v Use opticall
22. y clear microplates with flat wells for the best results Other microplates can be used as well consult the Biochrom website for a list of microplates that are approved for use with the Biochrom EZ Read 400 Microplate Reader Biochrom EZ Read 400 User s Manual Version 1 0 22 8 2 Quick Start Guide Print this guide and keep in the lab with the Biochrom EZ Read 400 Microplate Reader for easy reference To turn on the instrument 1 Connect instrument to a power source using the appropriate power cord Turn on the instrument using the switch at the back of the instrument 2 Check user s manual for important safety information 3 To connect the instrument to a PC 4 Connect to the PC via USB port Determine the communication port com used by the instrument In the Start menu of the PC go to Control Panel System Hardware Device Manager Ports 5 To connect instrument to ADAP software Insert the CD supplied with the instrument into PC install ADAP Open ADAP ADAP will prompt for a user ID and password Login using the pre set ID and password sadmin sadmin Once logged as sadmin specific user IDs passwords and administrative rights may be set Ei Instrument DEX File Ea se eec ae 6 Select Setup Instrument in the task bar iter Instrument am A dialogue box will open Wavelength 620 COM Port TOM4 S Send to Instrument Instrument Type Installed Plates na a 96 8x1
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