MH355 Semiautomatic Microtome Standard Setting List
Contents
1. e The knife centre gripping is fasten knife again 1 The section is uneven improper Move the knife holder lateral or The thin and thick section Knife is dull stick in new knife is alternate even if Pressure plate is broken or Change new holder plate or use sometimes it doesn t adjustment isn t right new knife holder cutaway e The cutting angle of the knife is Readjust holder plate too less Increase the cutting angle 12 gradually until find the optimum 2 The section is congestion and compressive The sections congestion There is the phenomenon of crease and nip Knife dull Specimen temperature is high Cutting speed so fast Use the other part of the knife or a new one Make the specimen cool before cutting Reduce the cutting speed 3 There is fringe in the section On the pressure plate is filling up paraffin on the back of the knife holder Clear up this regional paraffin 4 There is noise when cutting The knife will shake and sound when cutting some hard specimen There is pull or slightly frictional make on the knife 7 2 Instrument failure Cutting speed too fast The cutting angle is too big Clip of specimen or knife not fixed Turn hand wheel with a rather slow speed Decrease the cutting angle gradually until find the optimum one Check all the screwed and jaw connection in the specimen rest2. Setting values From 1um 10 um in 1pm increments From 10um 20um in 2 um increments From 20um 50um in 5 um increments From 50um 100um in 10 um increments From 100um 600um in 50 um increments 6 3 3 Retraction value 0 95um 5um increments 3 4 Specimen horizontal travel distance 25mm 3 5 Specimen vertical travel distance 60 mm 3 6 Power supply AC 220V 22V 50Hz 1Hz 3 7 Input power 50VA 3 8 Dimension L x W x H 620 x 410x310 mm 3 9 Total weight about 35kg 3 10 Working noise less than 65dB A 4 Working Conditions 4 1 This instrument is a movable desk top type which can be placed on the experiment stable working table to prevent it receiving quakes from the ground and don t install other vibrating equipments round it Adjust rubber knobs under instrument and stabilize it 4 2 Use USB cable to connect hand panel with communication port on the back of main machine 4 3 After confirming power with grounding wire three holes socket connect the instrument and power Socket by attaching wires Turn on the switch on the back of instrument and it is under state of working 4 4 This instrument uses single phase voltage 220V 10 and 50Hz AC power supply If does not meet requirement it needs to externally connect AC voltage stabilizer 4 5 The instrument should be used at ambient temperature 10 C 40 C 4 6 Environmental relative humidity shall not be more than 80 5 Operation 5 1 Turning on The instrument may on
3. if it had been dismounted Downwardly turn over the cutting edge cover sheet Turn the eccentric rod handle in the lateral of the body of revolution and draw it out from sideward Push the knife clamp back which have knife clip and shift it out from the rotary unit Turn the eccentric rod handle in the lateral of the knife clap and draw it out from sideward Dismount the knife clamp Clean up all parts of the knife holder Don t use xylene or alcoholic liquid e g glass cleaner when clean up paraffin Make the knife holder dry and assemble it together Apply to thin layer of lubrication after clean up the parts which had been taken off When fix the knife clip make sure that its upper part is parallel with the back edge of the knife clamp seat 8 1 4 Box shaped specimen grip Dismount the box shaped specimen grip to clear away the residual paraffin Don t use xylene or alcoholic liquid to clean up Use the substitute of xylene or paraffin scavenger You can put the box shaped specimen grip into oven to heat it to 65 C until lipid paraffin bleeding off Wipe off paraffin with dry cloth Apply oil to the axis grasping joystick after using oven heating method 8 2 Lubricate instruments Do oil lubrication for the following parts monthly 1 2 drop is well enough Instruments and specimen holder e Grip draw in the clamp Lock in iron at the T knife clap back of the microtome bedplate e The knife holder slide way
4. into the inside of machine during the cleaning Please follow the safety warnings of the manufacture when using cleaning solvent Put the hand wheel on the locking position when turn down the instrument Electronic parts be repaired by professionals and other people do not touch them 15
5. on the microtome bedplate Knife holder Lock in iron at the T body of the rotary units on the knife clamp seat The knife control grip is shift to the eccentric rod handle 14 The iron locking head on the knife clamp of the T body of rotary unit and the knife holder with slide way The grasping joystick of the knife Box shaped specimen grip The bearing of the grasping joystick 9 Notices Must grasp the specimen before positioning the knife During the operation locking hand wheel and cover the knife edge with its cover sheet Be most careful when you take the section knife It is possible to lead to bad hurt because of the sharp cutting edge You should turn the hand wheel with the same speed during the cutting process The hand wheel turning speed must suit with the hardness of the specimen Harder specimen use slow speed Locking hand wheel and covering the knife edge with its cover sheet when changing specimen piece The instrument should be positioned on the experiment work table level and stable Preventing it from quake from the ground and don t put other equipments which may produce vibration near the instrument Holding the fore and end trough of the back when moving it and don t hold the other parts like hand wheel handle Periodically cleaning the instrument Locking the hand wheel before cleaning Don t use acetone or xylene liquid to clean up the instrument Make sure that no paraffin comes
6. MH355 Semiautomatic Microtome Standard Setting List No Item Quantity 1 Main Machine 1 Set 2 Hand Wheel 1 Piece 3 Knife Support 1 Piece 4 Cassette Clamp 1 Piece 5 Scrap Trough 1 Piece 6 Hexagonal Wrench 1 Set 7 Power Line 1 Piece 8 Fuse Tube 5A 4 Pieces MOSS Instruments Co Ltd Date Registration No LS Shi YJX Zhun Zi 2009 No 1410018 Executive Standard YZB LS 0010 2009 MH355 Semiautomatic Microtome User Manual MOSS Instruments Co Ltd No 5 Hepanxiang Jinhua Zhejiang China 321001 TEL 86 579 8237 3070 FAX 86 579 8353 3009 www mossinstruments com Contents Profitable SUGGESTIONS ia e e RA PEOR ce NILUM SL elt UL T UR 1 1 Introduction eerta needed vei dde ee iate ye Ue eds 4 2 Scope oL Application 1 2 o Sentus oe intel IIR HR ERU I EE M B ee 6 3 Technical Parameter erm eme obe ss d a d n e nannies 6 4 Working Conditions icecream nerian ania thiet piede d een dept e a deci dd p ded end 7 5 Operatioh ey eee eet du rede iri der oh re erre e ee de os det eese dere da dais ginta 7 5 1 LUMINI ON disset ie etate ette tov mu Du tne a uestem NG 7 52 Hand panel operation RR td tei te Fe b e Fe tendra 7 bi3ASectioning arm ptompL s ucc eir ier drea rt re e eee ve eer ia a 8 5 4 Normal section or trim operation prompt sse 8 b 5 Error code prompt e iet de t e re e bea ert Fee da ere Fe e eee eee e eee Fees 8 5 6 Main machine panel o
7. and knife holder system If necessary fixture the control rod and screw 1 No section is cut when turn the hand wheel e The specimen has reached to the extreme position Press back key to make the specimen backward and so the knife holder 2 Knife service time is short The power for section is too strong Adjustment the cutting speed or the section thickness in the process of cutting Choose smaller section thickness or slow down speed of turning hand wheel 8 Cleaning and Maintenance 8 1 Cleaning up the instruments 8 1 1 Conduct the following steps before cleaning each time Turn up the specimen grip to the top and lock the hand wheel Release the specimen grip and pull it out Pick off the knife from the knife holder and put it back to the knife box Dismount the knife holder and its seat to clean up Take down the specimen grip to clear up separately 13 Take down the specimen from the specimen nip Clear away the section waste with dry brush 8 1 2 Instruments and external surface If necessary the external painted surface can be cleaned with light duty commercial housework cleaner or suds And then use wet cloth rub it until dry You may use the substitute of xylene paraffin oil paraffin scavenger to erase residual The instruments must be dry when use again 8 1 3 Knife holder Please according to following steps to clean up the knife holder
8. de button This button may repeatedly use First press it Yn indicator lights up and displaying screen displays number of total previously completed section The instrument automatically adds on number Second time press it gt um indicator lights up and displaying screen displays number of total thickness in um Number automatically changes according to thickness of per piece and total pieces Third time to press it system goes to retraction retract indicator lights up and screen displays retraction value To change the display mode press DISPLAY MODE until the LED of desired mode is illuminated When the instrument is switched off using the main power switch both values section thickness sum and section number are erased from memory 9 6 2 CLEAR Press Clear button to reset section thickness sum or section number back to 0 5 6 3 Retraction Press DISPLAY MODE and CLEAR buttons simultaneously to call up the retraction function The retraction value can be adjusted by using and button on the main panel in increments of 5pm to maximum of 95um After reset press CLEAR button to confirm desired retraction value While the specimen is in retraction the red LED on the RETRACT lights up 6 Parts adjustment 6 1 Specimen holder and orientation system Orientation system Specimen holder clamp Knife holder Scale for positioning Fig 6 Lock and loose Orientation specimen holder Pull
9. eccentric rod to lock or release specimen box Locking lever Turning the locking lever Fig 7 to set the specimen holder to the adjustable release condition and section lock status 10 Turning the two orientation knobs under release condition make specimen holder clamp plane defluxion by pass horizontal axis and vertical axis for ensuring required tangent plane location to decide the required plane cutting location Turning the adjustable lever to lock and loose specimen holder clamp 6 2 Box shaped specimen clamp e Turning the spanner Fig 8 on the specimen clamp can make the jaw in the state of braced and locked The specimen can be put in or take off in the state of braced Specimen box may be placed horizontally or vertically 6 3 Knife holder Protective guard Release adjustable lever to move knife RA Y SG holder left and Sl Y SU AIR Pull knob to lock right and loose blade qp Use 4 hexagon spanner manually rotate the knife holder to adjust slice angle scale is for reference Fig 9 To turn the adjustable lever under knife holder to release and lock knife holder base In release conditions you can make the knife holder base do the back and forth movement by hand to choose latched position needed There is a scale under knife holder for reference by location Use hexagon spanner to turn the eccentric rod in the hole on the right side of the knife holder to release and lock the rota
10. initializing 5 2 2 Press button to increase sectioning or trimming value in the range of setting 5 2 3 Press button to decrease sectioning or trimming value in the range of setting 5 2 4 When pressing a sectioning arm goes backwards 5 2 5 When pressing sectioning arm goes forwards ot When the instrument is carrying on 1 or 4 operation if feeding arm arrives at limitation there is 5 3 Feeding arm pro long prompt beep with hand box 5 4 Normal section or trim operation prompt When rotates hand wheel to do section or trim if feeding arm arrives to forward limitation there is long prompt beep with hand box If continue to rotate hand wheel alarm will not stop but feeding arm will not go forwards any more 5 5 Error code prompt If after switch on instrument LED screen on the main machine displays EO or LED screen on the hand box displays EO there is communication problem between main machine and hand box Please switch off power first then replug cable Switch on power again 5 6 Main machine panel operation SN Total pieces indicator Ga um Total thickness indicator nee Retraction indicator PES i Setting indicator Display Mode Clear Button Switches between section DISPLAY To reset section counter thickness sum and ODE CLEAR and section thickness section counter sum back to 0 Fig 5 main machine panel layout DISPLAY 5 6 1 MODE Mo
11. is the national regulated first class medical equipment It is a device used for human and propagation tissues pathological section analysis It is used to cut paraffin embedding specimens thin section mounted specimens It can be widely used for pathological diagnosis analysis and research in hospitals medical colleges legal medical experts and propagation institutes The instrument mainly consists of the following Box type specimen clamp Direction movable support for the specimen clamp Fore and aft movable knife holder and locking device Removable waste tray Microtome hand wheel and locking device Seat and driven mechanism on it Cover Electronic control system Operating and displaying board on the cover Separate operating and displaying control panel General overview Cover Orientation system Cassette Clamp Main machine Panel Knife holder Base plate Control Panel Fig 1 Handwheel Locking lever Lever for knifer holder moving Waste tray Fig 2 Fuse Switch Plug Control panel plug Fig 3 2 Scope of Application Used to cut paraffin embedded specimen and make thin section mounted specimens 3 Technical Parameter 3 1 Section thickness 1um 100um adjustable 3 5um is the best result Setting values From1 um 20um in 1 um increments From 20um 60um in 5 um increments From 60um 100um in 10 um increments 3 2 Trimming thickness 1um 600um adjustable
12. ly be connected to a grounded mains power outlet socket To connect the instrument only use one of the mains cables that are supplied together with A the instrument Turn on switch at the rear of instrument refer to Fig 3 after instrument buzzing and instrument initializes The feed arm return to original position Hand panel displays and main machine panel displays After initialize follow by short beep to remind that instrument finishes initialize Instrument is ready for work LED on hand panel displays with xx x sectioning value and is in unit of micron Main machine panel displays total pieces as 0 xx x is last adjusted sectioning value Factory set value is 5 0um 5 2 Hand panel operation Layout of this instrument is as Fig 4 Above LED displays working mode and setting value separately Below is operational buttons LED displ f LED TRIM red EN oos due La NOT PT Lights up when trimming mode is activated sectioning and trimming TRIM Mee SERT LED TRIM red pl Lights up when sectioning mode is activated lt t 2 Button for feeding backward P4 SR Button to increase SECTA Button to decrease thickness TE ai TRIM J thickness gt Button for feeding forward Fig 4 Hand panel layout 5 2 1 Sectioning and trimming mode switch Press button in the middle of hand panel to switch sectioning mode to trimming mode en He Instrum ault mode is sectioning model after
13. peration cccesssssesscessecscessceecesssnsoescecsssssseoseseecenssseesesessssesseeeeseaeetesaeseeeeneenes 8 6 Parts ad j stmeht cedendo CHE Ee D die ter ae ettet e ee dee ei deals 10 6 1 Specimen holder and orientation system nennen 10 62 Boxshaped specimen clamp ie te e te e SEE 11 6 3 Knife holder s Red e ata 11 o4 Hand wheel octets rete ree A lee etes Saeed re oh terior 12 7 Normal Troubles Solving Methods essere eene 12 8 Cleaning and Maintenance sse nre nennen nenne nennen 13 8 1 Cleaning up the instruments sss nennen ennt netten etre a 13 8 2 Lubricate instruments nter tete e teret pe ee ed SEES Fre ERNE eee rege ee TOT 14 9 NOlICGS NANA 15 Profitable Suggestions The user should read this user manual carefully before installation and operating the machine Check your power socket is well grounded Blade is sharp beware when you change it Do not place blade anywhere with the cutting edge facing upwards Before changing specimens always lock the hand wheel and cover the knife edge with the knife guard Do not put instrument under extreme temperature and high air humidity environment Failure to follow this will cause instrument severe damage Please keep instrument far away from fire Incase of malfunction contact our company Don t try to solve it by your own risk 1 Introduction Model MH355 paraffin microtome
14. tor of the knife holder In release condition you can move the rotator by hand to choose locked cutting angle needed There is a scale on the right side for reference by location 11 Turn adjustable lever on the rotator to release and lock knife clamp In release condition you can make the knife clamp do the left and right movement by hand to choose latched position needed Turn adjustable lever on the knife clamp to release and lock knife flat You can put in or take off the knife in release conditions Take off the knife after finish the work The protecting plate shall be on the installed position when the knife is on the knife clamp 6 4 Hand wheel Hand wheel can be released or Hand wheel locker Spanner locked status may lock at top position Hand wheel can be locked at a specific position when the spanner on the hand wheel is turned Rotating signal Turn the eccentric rod handle i under hand wheel to lock that at any Adjustable lever is to lock p BEN the wheel at any position position Where there is a solid circle is locked where there is hollow circle Positioning signal is rotatable 7 Normal Troubles Solving Methods There are normal problems in the following table which are likely to happen when the instrument is used Besides there are some possible causes that lead to these problems happened and solving methods Trouble Reason Solving Methods 7 1 Possible phenomena
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