H101-K-Frame User Manual
Contents
1. Figure 7 1 Insertion of the ring 2 insertion of the 35 mm dish 3 insertion of the magnetic lock NOTE Spacing rings are included with sample holder www oko lab com page H101 K FRAME User Manual March 2015 2 2 BD Falcon 35x10 Willco 35x10 Figure 8 Ring selection scheme for 35 mm dish oS Grainer Petri35x10 Corning 35x10 mm C Corning 60x15 mm www oko lab com Ibidi p Dish 35 mm low MatTek P35G X14 X Ibidi p Dish 50 mm low MatTek P50G X30 X S BD Falcon 60x10 Figure 9 Ring selection scheme for 60 mm dish Willco 60x15 Grainer Petri 60x15 ch Page9 H101 K FRAME User Manual March 2015 6 Working with 1x3 and 1x2 chamber slides magnetic locks Magnetic locks prevent movement of 1x3 and 1x2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder Figure 11 Magnetic lock for 1x2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 page LO www oko lab com H101 K FRAME User Manual March 2015 7 Working With MW Plates Magnetic Locks And Chamber Riser Magnetic locks hold MW plates in place into sample holder illustrated in Figure 12 NOTE Magnetic locks must be ordered separately NOT INCLUDED Product codes MW LOCK 22 5 and MW LOCK 16 5 MW 22 5 LOCK To be ordered separately Typically us2. chamber slide For 2 1 x3 chamber slide For 1 Lab Tek Il1 x2 chambered 1 60mm petri dish cover glass and 1 60mm petri dish 1x60 M 1xLABTEK M 1xLABTEK II M 2x35 M 2xLABTEK M For 1 35 mm petri dish For 1 Lab Tek 1 x2 chambered For 1 Lab Tek II 1 x2 chambered For 2 35 mm petri dishes For 2 Lab Tek 1 x2 chambered cover glass cover glass cover glass A 90 9 2xEABTEK H M LABTEK H 35 M EABTEK 35 M H104 4xF25 M HTOt6MW For 2 Lab Tek For 1 Lab Tek II 1 x2 chambered For 1 Lab Tek 1 x2 chambered For 1 Nunc and Greiner For 1 6 well plates holder 1 x2 chambered cover glass cover glass and 2 35mm petri dishes cover glass and 2 35mm petri dishes T25 flask holder ee ve Ah ht AY di p D G H101 TZ2MW H101 Z4MW H101 96MW H101 24 NUNC MW H101 48MW For 1 12 well plates holder For 1 24 well plates holder For 1 96 well plates holder For 1 24NUNC GREINER well For 1 48 well plates holder plates holder Figure 2 Available sample holders with corresponding lid adapters www oko lab com pee H101 K FRAME User Manual March 2015 Lid Adapter Figure 3 Assembly Lid Adapter to the Lid Chamber 3 Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located
3. in the H101 K FRAME see Figure 4 Front and 3D views Temperature Sensor dedicated opening 1 Frontal view 2 3D view Figure 4 Insertion of the temperature sensor inside the chamber 4 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold er To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the chamber base as illustrated in Figure 5 www oko lab com s H101 K FRAME User Manual March 2015 Insert the adapter by superimposing the red points Figure 5 Insertion of the Sample Holder inside the Chamber Base www oko lab com ew H101 K FRAME User Manual March 2015 5 Working with 35 or 60 mm Petri Dishes Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder Figure 6 illustrates the available magnetic locks for 35 and 60 mm dishes Threaded magnetic posts allow adjusting holder s height Figure 6 Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings accounting for variability in dish diameter from one manufacturer to another are also included Figure 7 shows how to position the ring Ring selection guide diagrams rings for 35 and 60 mm dish Figure 8 and Figure 9 re spectively
4. H101 K FRAME User Manual March 2015 H101 K FRAME Compatible with the following XY stages Compatible with the following Okolab Controllers e ASI all models including z piezo H101 BASIC BL Leica all models with 160x110mm opening H101 CRYO BL Ludi all models with 160x110mm opening Marzhauser all models Olympus all models with 160x110mm opening Prior H107 Zeiss all models with 160x110mm opening www oko lab com Page 1 H101 K FRAME User Manual March 2015 Index Me Component and CUMS SIONS EE 3 2 Sampe Nolder and Od EE 4 2 1 Available Sample e 4 3 Insertion of the Sample Feedback Temperature Sensor AAA ENEE EEN EE EE EEN 6 4 insercvion of Sample Holder into EE eege a 6 5 Working with 35 or 60 mm Petri Dishes Spacing Rings and Magnetic Locke ae EEN 8 6 Working with 1x3 and 1x2 chamber slides magnetic lOCKS ccecccecccccccecccccccecccecccecccecccecces 10 7 Working With MW Plates Magnetic Locks And Chamber Riser ccccccccccccccccecccccccecccecccecccecccecees 11 Be CONMECEONS GOSCh IP CIO EE 12 WORMS With Cr OT Cl END 13 10 Connecting the Chamber with XY stage sesessesescecescoecesoecesoecesoecesoecesoecesoecesoeceseeceseeceseeceseeces 14 www oko lab com es H101 K FRAME User Manual March 2015 1 Components and dimensions H101 K FRAME includes the following components Chamber main body it is uniformly heated by me
5. ans water circulation in water tight channels Cover glass lid It is uniformly heated by means water circulation in water tight channels Chamber riser It is a removable frame increasing the height of the chamber from 26 to 32 mm often re quired when using multi well MW plates Lid Adapter a Distance between focal plane and upper side of the glass lid with the chamber riser Lid 26 Distance between focal plane and upper side of the glass lid without the chamber riser o Sample Holder Order Separately 190 20 Chamber Riser Chamber top view gt Chamber Base Figure 1 H101 K FRAME Components and Dimensions www oko lab com 144 20 pape H101 K FRAME User Manual March 2015 The following sample holders are available H101 K FRAME features a lid adapter for each sample holder included in the sample holder code Lid adapter fit into the chamber lid and is held in place by magnets embedded within both chamber lid and lid adapter as illustrated in Figure 3 Point A Figure 2 shows the available sample holders and the corresponding lid adapters NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1 35mm Petri dish 1 1x3in chamber slide 1 Lab Tek 1x2 chambered cover glass 1 Lab Tek II 1x2 chambered cover glass 1 60mm Petri dish 1 Nunc and Greiner T25 flask 2 35mm Pet
6. com el 2 H101 K FRAME User Manual March 2015 9 Working with Perfusion The Chamber Riser included with H101 K FRAME features 12 perfusion holes for the insertion of perfusion tubing up to 2 5 mm in outer diameter Small screws plug the perfusion holes when not in use Grub screws M3x6 Remove small screws as needed before introducing perfusion tubing Figure 16 shows location of perfusion holes 1 Sensor Perfusion dedicated opening 1 Frontal view 2 3D view Figure 16 Perfusion www oko lab com bape 3 H101 K FRAME User Manual March 2015 10 Connecting the Chamber with XY stage H101 K FRAME fits on the XY Stage without any screws To fix the chamber to the stage follow the steps shown in Figure 17 1 Place the chamber on the stage See Image 1 of Figure 17 2 Tilt the chamber and slide it towards the spring until it snaps in place See Image 2 of Figure 17 3 Image 3 of Figure 17 shows the chamber installed on the XY Stage Figure 17 Connecting the Chamber with XY Stage www oko lab com page 14
7. ed for 6 12 24 48 MW MW 16 5 LOCK To be ordered separately Typically used for 96 MW Figure 12 Magnetic locks for MW plates To be ordered separately Chamber Riser increases chamber s height and is REQUIRED with 6 12 24 48 MW plates regardless of the Lid em ployed Figure 13 shows how to install and fasten the chamber riser Screws location is indicated by letter A Chamber Base Figure 13 Riser Assembly www oko lab com bape 1 H101 K FRAME User Manual March 2015 8 Connectors description 1 Gas Input A single silicon tubing carries output gas from the Okolab Bubbler Column to the H101 K FRAME Silicon tubing connects to a gas input brass opening located on a corner of the H101 K FRAME See Figure 14 Point 1 Connect by gently pushing silicon tubing onto brass opening 2 Water Input and Output Silicon tubing carry output and input water from the thermostatic bath to the H101 K FRAME See Figure 14 Points 2 Connect by gently pushing silicon tubing onto steel opening Match colors on chamber steel openings with ones on the thermostatic bath 3 Water Input and Output A single silicon tubing carries output water from the lid water jacket to the base one See Figure 14 Points 3 The silicon tubing connects the base and lid as shown in Figure 15 Figure 14 Connectors Figure 15 Connecting tubing between base and lid water jacket www oko lab
8. ri dish 2 1x3 chamber slides 2 Lab Tek 1x2 chambered cover glass 2 Lab Tek Il 1x2 chambered cover glass 2 60mm Petri dish 4 35mm Petri dish 1 1x2 chamber slide and 2 35mm Petri dish 1 Lab Tek 1x2 chambered cover glass and 2 35mm Petri dish 1 Lab Tek II 1x2 chambered cover glass and 2 35mm Petri dish 1 Lab Tek 1x2 chambered cover glass and 1 60mm Petri dish 1 Lab Tek II 1x2 chambered cover glass and 1 60mm Petri dish 1 1x3 chamber slide and 1 60mm Petri dish 1 60mm Petri dish and 1 35mm Petri dish 6 well plates one MW LOCK required 12 well plates one MW LOCK required 24 well plates one MW LOCK required 24 well NUNC GREINER plates one MW LOCK required 48 well plates one MW LOCK required 96 well plates one MW LOCK required It includes the insert for oil immer sion imaging Magnetic lock to hold the standard MW plates in the chamber Magnetic lock to hold the low profile MW plates in the chamber www oko lab com Page H101 K FRAME User Manual March 2015 99 E 99 G G Ae 2ECM GS35 M 2x60 M GS60 M LABTEK 60 M For 4 35 mm petri dishes For 1 1 x3 chamber slide and For 2 60 mm petri dishes For 1 60mm petri dish and For 1 Lab Tek 1 x2 chambered 2 35mm petri dishes 1 1 x3 chamber slide cover glass and 1 60mm petri dish 1x35 M 6035 M 1xGS M 2xGS M LABTEK II 60 M For 1 35 mm petri dish For 2 35 mm petri dishes and For 1 1 x3
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