Avanti® JE Centrifuge - USF Research & Innovation
Contents
1. YN 18 000 2 C JA 20 1 Fixed Angle 23 32 place Fmax 115 mm 20 1 20 000 51 500 32 x 15 mL J TB 022 outer row Imax 98 mm 17 000 43 900 inner row 2 C JA 20 Fixed Angle 34 8 place lud Tmax 108 mm 20 20 000 48 400 8 x 50 mL J TB 003 u 17 000 2 C er JA 18 Fixed Angle 23 10 place Tmax 132 mm 18 16 000 37 800 10 x 100 mL J TB 035 14 000 2 C JA 17 Fixed Angle 25 6 place Tmax 132 mm 17 17 000 39 800 14x50 mL J TB 017 15 000 2 C rj Maximum speeds are based on a solution density of 1 2 g mL in all rotors b Maximum speed for the rotor in the Avanti J E centrifuge At 35 C ambient temperature and 95 percent humidity a Maximum speed in the Avanti J E for the rotor with magnets without magnets maximum is 14 000 rpm Maximum speed at 2 C in a 50 Hz centrifuge is 14 000 rpm Maximum speed for rotor without magnets is 13 000 rpm o Temperature performance for the F14BCI 14x50cy rotor in the Avanti J E is as follows 12 C minimum at 14 000 rpm at 35 C ambient 4 C minimum at 13 000 rpm 35 C ambient Maximum speed for rotor without magnets is 6 300 rpm I Description Rotor Rotor Entry Max Max RCF Max Manual Rotor Profile and Description Code RPMa x g2. 4 4 MAINTENANCE Instrument CE e ge 5 1 EA PUN e bene d 5 2 Cleaning 2 0 gs sea La aus ER Educ eed 5 2 Decontamination 5 3 Sterilization and Disinfection 5 3 Replacing the Air Filter 5 4 Circuit Breaker and Fuses 5 5 Storage and Transportation 5 5 SIOTAge LE ensure horse hu ee eee le 5 5 Supply LISE see mme ente ep Oe ep S ame 5 6 Replacement Parts 5 6 Supplies tiro da a Oe Adal a at 5 6 Illustrations and Tables Figure 1 1 Figure 1 2 Figure 2 1 Figure 2 2 Figure 4 1 Figure 5 1 Table 4 1 Page The Avanti J E Centrifuge 225 urn 1 1 Control Parla aa Ban 1 4 Dimensions of the Avanti J E Centrifuge 2 2 Single Phase Power Connection 2 3 Manual Door Release 4 6 Air Filter Replacement 5 4 Diagnostic Message Chart 4 2 CERTIFICATION SCOPE OF MANUAL Introduction To ensure full system quality Beckman Coulter Avanti J E centri fuges have been manufactured in a registered ISO 9001 or 13485 facility They have been designed and tested to be compliant when used with Beckman Coulter rotors with the laboratory equipment requirements of
3. Height door open 152 4 cm 60 in Weight seeded tetro CREATE et ds 267 4 kg 589 Ib Ventilation clearances Ja PE EE 7 6 cm 3 0 in Rear eub Loewe eva 16 0 cm 6 25 in Finishes Control panelet ierse Hrn silicone elastomer Top surface eer ne nenne urethane paint Others rfaces an are acrylic baking enamel Electrical requirements 208 240 V instrument 187 229 216 264 VAC 24 A 60 Hz 230 V instrument 207 253 VAC 24 A 50 Hz 200 V instrument 180 220 VAC 24 A 50 Hz Electrical supply 4 2 a RR NEG Class I Maximum heat dissipation into room under steady state conditions 6 900 Btu hr 2 kW Noise level 0 91 m 3 ft in front of centrifuge at 21 000 rpm lt 64 dBa Installation overvoltage category II Pollution degree a ern nennen ER des 2 Normally only nonconductive pollution occurs occasionally however a temporary conduc tivity caused by condensation must be expected Description AVAILABLE ROTORS Refer to the applicable rotor manual for complete rotor descriptions Rotor Rotor Entry Max Max RCF Max Manual Rotor Profile and Description Code RPMa xg Capacity Number JA 25 50 Fixed Angle 34 8 place Tmax 108 mm 25 5 210005 53 300 8 x 50 mL J TB 056 18 000 2 C JA 21 Fixed Angle 40 18 place my Tmax 102 mm 21 21 000 50 400 18x10 mL J TB 002
4. 233679 STARTING A PROGRAMMED RUN Action 1 Press PROGRAM 2 Use the keypad to enter the number of the program then press ENTER Result Previously saved program numbers are displayed The message Press ENTER START to run or edit parameters 1s displayed Action 3 Press SHOW SET 4 Press ENTER and START to begin the programmed run or press PROGRAM to exit program mode and return to manual operation 5 After the rotor has stopped press DOOR 6 Remove the rotor Keep the chamber door closed between runs Operation Result The number you selected will appear in the program display Run parameters for that program will be displayed for 5 seconds To make changes see Changing a Program below The run starts the green light next to the START key flashes and the rotor begins to spin I gt NOTE To begin a run ENTER must always be the last key pressed before pressing START If you wait more than 5 seconds the START key will not activate If this happens press ENTER and START again to begin the run If an unacceptable value was entered the run will not begin when ENTER and START are pressed Check each display and make any necessary corrections or additions Press ENTER and START again to begin the run The run will end automatically when the time display counts down to Zero Press STOP to terminate a run at any time The green light ne
5. and Percoll The Avanti J E is microprocessor controlled providing interactive operation The instrument design features a brushless switched reluctance drive motor a temperature control system with automatic temperature compensation unique for each compatible rotor and selectable acceleration and deceleration rates Manual and programmed operation are available n manual operation you enter the individual run parameters before beginning each run n programmed operation you can duplicate runs quickly and accurately by selecting one of nine programs previously entered into the program memory User messages and or audible signals are provided to alert you to conditions that may need attention The instrument housing is made of steel and molded structural foam The door and structural foam cover panels are finished with polyure thane enamel The steel and structural foam door is hinged at the back providing clearance for loading and unloading of the centrifuge In the event of a power failure the door can be manually unlocked for sample recovery see Section 4 TROUBLESHOOTING Manufactured under license from Switched Reluctance Drives Limited Harrogate U K 1 2 ROTOR CHAMBER VACUUM TEMPERATURE CONTROL Description The rotor chamber is made of stainless steel to resist corrosion A rubber gasket around the chamber opening ensures sealing Instrument gaskets have not been qualified as bioseals for ae
6. hours and minutes The time display begins counting down when START Is pressed and continues counting down to 0 when deceleration begins The run automatically ends when the set time reaches zero a tone sounds when the rotor stops spinning If a malfunction shuts down the centrifuge during a run the time counting stops and the time remaining when the run stopped remains in the display By comparing this time with the original set time you can determine when the run ended Ina hold run indicates the time elapsed since START was pressed After 99 hours and 59 minutes the timer resets to 0 and continues counting elapsed time Indicates rotor temperature in degrees C at thermoequilibrium The displayed temperature is within 2 degrees of the set temperature after equilibration Displays the name of the installed rotor When the ACCEL key is pressed an acceleration rate 1 MAX 2 SLOW can be entered If no number is entered the instrument accelerates at the rate set for the previous run When the DECEL key is pressed a number representing the selected deceleration rate 1 MAX 2 SLOW 3 OFF can be entered If no number is entered the instrument decelerates at the rate set for the previous run 1 5 Description 1 6 O PROGRAM Run Parameter Keys ROTOR 0 RPM RCF TEMP C During setup when the PROGRAM key is pressed a program number one of nine possible can be entered Du
7. solutions and test samples that are pathogenic toxic or radioactive Such materials should not be used in this centrifuge however unless all necessary safety precautions are taken Observe all cautionary information printed on the original solution containers prior to their use Handle body fluids with care because they can transmit disease No known test offers complete assurance that they are free of micro organisms Some of the most virulent Hepatitis B and C and HIV I V viruses atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this centrifuge without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection e Dispose of all waste solutions according to appropriate environmental health and safety guidelines It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter field service SJ IM 8 gt August 2009 BECKMAN COULTER Avanti J E Ce
8. through S14 System error call service There is a problem with the system control soft ware EPROM or RAM System shuts down Call Beckman Coulter service H1 H5 H7 and H8 Speed call service Speed control problem Run shuts down with maximum brake door may not unlock for up to an hour Call Beckman Coulter service H2 H3 and H11 Speed call service Speed control problem Run stops usually with no brake Call Beckman Coulter service H4 H6 and H9 Speed error Accel or decel speed problem Run continues Press CE to clear message 11 Rotor imbalance Rotor load is severely out of balance Run shuts down with maximum brake Make sure that tubes or bottles are loaded symmetrically in the rotor With swinging bucket rotors remove the buckets and lubricate the pivot pins where the buckets contact as described in the applicable rotor manual Unlubricated pivot pins can prevent the buckets from reaching horizontal position which can cause imbalance During low tempera ture runs near 10 C ice forms around the door opening Door will not open at the end of a run To minimize icing wipe moisture from the chamber the chamber gasket and the inner door surface before each run Keep the door closed as much as possible RETRIEVING YOUR SAMPLE IN CASE OF POWER FAILURE If facility power fails only momentarily the instrument will resum
9. time display counts down to zero Press STOP to terminate a run for any reason The green light next to the STOP key will flash while the rotor decelerates A tone sounds when the rotor comes to a complete stop The door is unlocked PROGRAMMED OPERATION The instrument internal memory can store up to nine programs which can be recalled by keypad numbers 1 through 9 Saved programs are retained in memory even if the power is turned off Procedures for entering run parameters are the same for programmed operation as those described above for manual operation 3 9 Operation CREATING A NEW PROGRAM Action 1 Press PROGRAM 2 Use the keypad to select a program number that is not in use or one that you want to overwrite and press ENTER 3 Enter run parameters speed time temperature accelera tion deceleration rate settings 4 When all parameters are entered press SAVE 5 Press ENTER START to run the programmed run or press PROGRAM to exit program mode and return to manual operation Result Previously saved program numbers are displayed The message Press ENTER START to run or edit parameters 1s displayed If you entered an unacceptable parameter an error message will be displayed Press CE and enter an acceptable value then press the next run parameter key The program is saved into memory gt NOTE Record the program parameters on the Program Library record
10. wire extension cord or a two wire non grounding type of multiple outlet receptacle strip Do not place containers holding liquid on or near the chamber door If they spill liquid may get into the centrifuge and damage electrical or mechanical components Safety Against Risk of Fire Fuses protect certain electrical circuits within this instrument against overcurrent conditions For continued protection against the risk of fire replace only with the same type and rating specified This centrifuge is not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials such as chloroform or ethyl alcohol in this centrifuge nor handle or store them within the required 30 cm 1 ft area surrounding the centrifuge Mechanical Safety For safe operation of the equipment observe the following Use only the rotors and accessories designed for use in this centrifuge Do not exceed the maximum rated speed of the rotor in use Do not lift or move the centrifuge while the rotor is spinning NEVER attempt to slow or stop the rotor by hand NEVER attempt to override the door interlock system while the rotor is spinning Inthe event of a power failure do not attempt to retrieve the sample from the centrifuge for at least 1 hour Then follow the instructions for sample recovery in Section 4 TROUBLESHOOTING Chemical and Biological Safety Normal operation may involve the use of
11. 0 Available Rotors oia et beds bos 1 11 SECTION 2 PREINSTALLATION REQUIREMENTS Space Requirements 2 1 Electrical Requirements 2 3 Securing the Centrifuge to the Floor 2 4 Bio Safety Level 3 Installation 2 5 111 Contents iv SECTION 3 SECTION 4 SECTION 5 Warranty Page OPERATION Summary of Run Procedures 3 2 Programmed Run 3 2 Manual RUN nt mann 3 3 Pr paration test e etse ette tede ec edt idos 3 3 Installing the Rotor 3 4 Manual Operation 3 5 Selecting a Rotor 3 5 Entering Run Speed 3 6 Entering Run Time 3 6 Entering Run Temperature 3 7 Entering Acceleration and Deceleration Rates 3 8 Starting a Run 2a ee RR HERR ath eka nahh di 3 9 Programmed Operation 3 9 Creating a New Program 3 10 Starting a Programmed Run 3 10 Recalling and Changing a Program 3 11 TROUBLESHOOTING UserMessdges a v Sw en eda RN ed TE 4 1 Retrieving Your Sample in Case of Power Failure
12. 5 and 2 the device is not to be disposed via the municipal waste collection system of any member state of the European Union Itis very important that customers understand and follow all laws regarding the proper decontamination and safe disposal of electrical equipment For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection treatment recovery recycling and safe disposal of the device DESCRIPTION CENTRIFUGE FUNCTION 1 Description The Avanti J E high performance centrifuge see Figure 1 1 is a refrigerated centrifuge that generates centrifugal forces required for a wide variety of applications Together with the compatible Beckman Coulter rotors applications include Routine processing such as sample preparations pelleting extrac tions purifications concentrations phase separations and spin column and spin filter centrifugations Figure 1 1 The Avanti J E Centrifuge 1 1 Description HOUSING AND DOOR Registered trademarks of Pharmacia AB Rapid sedimentation of protein precipitates large particles and cell debris Preparation of subcellular organelles such as mitochondria nuclei chloroplasts and crude microsomes Separation of blood cells and cellular components Gradient separation for example Ficoll Hypaque
13. Capacity Number JLA 16 250 Fixed Angle 25 6 place Tmax 134 mm 16 25 16 0004 38 400 6x250mL J TB 072 E 14000 2 C JA 14 Fixed Angle 25 6 place Tmax 137 mm 14 14 000 30 100 6x250mL J TB 004 F14BCI 14x50cy Fixed Angle 34 f Ma plete rmax 153mm 50 14000 33500 14x50mL F14BCI 6x250y Fixed Angle 23 6 place Sea Tmax 137 mm 250 14 000 30 000 6 x 250 mL max JA 12 Fixed Angle 35 12 place Tmax 144 mm 12 12 000 23 200 12x50 mL J TB 051 JA 10 Fixed Angle 25 6 place Tmax 158 mm 10 10 0008 17 700 6x500 mL J TB 006 m o d o e g a 50 Hz centrifuge is 14 000 rpm 4 C minimum at 13 000 rpm 35 C ambient Maximum speeds are based on a solution density of 1 2 g mL in all rotors Maximum speed for the rotor in the Avanti J E centrifuge At 35 C ambient temperature and 95 percent humidity Maximum speed for rotor without magnets is 13 000 rpm Maximum speed for rotor without magnets is 6 300 rpm Maximum speed in the Avanti J E for the rotor with magnets without magnets maximum is 14 000 rpm Maximum speed at 2 C in Temperature performance for the F14BCI 14x50cy rotor in the Avanti J E is as follows 12 C minimum at 14 000 rpm at 35 C ambient Description Rotor Max Rotor Entry Max RCF Max Manual Rotor Profile and Description Code RPMa xg Capacity Number
14. Chemical Resistances for chemical compatibilities of centrifuge and accessory materials Flammability hazard Do not use in or near operating centrifuges 5 3 Maintenance While Beckman Coulter has tested ethanol 70 and found that it does not damage the centrifuge no guarantee of sterility or disinfec tion is expressed or implied When sterilization or disinfection is a concern consult your laboratory safety officer regarding proper methods to use REPLACING THE AIR FILTER Check the air filter Figure 5 1 regularly and replace it about once a year or more often if it looks dirty The air filter is not fastened to the centrifuge so no tools are required for removal or installation 1 To remove the air filter grasp the top edge and lift the filter stralght up 2 Install a new filter by holding the top edge and inserting the bottom edge into the slot in the air deflector strip Lower the filter until the bottom edge rests on the brackets Se gt SISIIISISIIS SES gt SSSSSS cS SS SSS SSoSSS 5 233S3 S lt SSO eS gt SSSS SS SSS X X gt SSIS Ss SSS X3 SSS ISISI X ESS SII Is sS SG SSSSES S BW SES Air Deflector Filt
15. Coulter service maximum brake C3 Temp call service Cannot maintain temperature Run shuts down with maximum brake Check the air filter and replace if dirty see Section 5 MAINTENANCE Call Beckman Coulter service Continued Troubleshooting Table 4 1 Diagnostic Message Chart continued Diagnostic Number Message Problem Result Recommended Action C5 Temp call service Refrigeration system error Run shuts down with maximum brake Call Beckman Coulter service T1 through T4 Temp call service System temperature problem Run shuts down with maximum brake Call Beckman Coulter service D1 through D12 D14 and D15 Drive call service Drive system problem Run stops usually with no brake Door may not unlock for up to an hour Call Beckman Coulter service Before trying to open the door listen carefully and make sure that no sound is coming from the chamber indicating a spinning rotor Follow the directions under ACCESSING THE ROTOR IN CASE OF POWER FAILURE below D13 No rotor in chamber or drive problem There is no rotor installed or the drive belt is loose or broken Run shuts down with maximum brake Install rotor per the applicable rotor manual If the rotor is installed when the message appears call Beckman Coulter service Fl and F2 FRS call service Required vacuum level not reached in
16. F ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT ABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CON SEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT BECKMAN COULTER Beckman Coulter Inc 250 S Kraemer Blvd Brea California 92821 Sales and Service 1 800 742 2345 Internet www beckmancoulter com 02009 Beckman Coulter Inc All rights reserved
17. JLA 10 500 Fixed Angle 20 6 place e Imax 166 mm 10 5 100003 18 600 6 x 500 mL J TB 048 F10BCI 6x500y Fixed Angle 23 6 place e piae fmax 158 mm 500 10000 17 696 6 x 500 mL JLA 9 1000 Fixed Angle 20 4 place Imax 185 mm 9 1 6 300 gt 8230 4 x 1000 mL J TB 073 JS 13 1 Swinging Bucket 6 place max 140 mm 13 13 000 26 500 6 x 50 mL J TB 036 al 12000 u 2 C JS 5 3 Swinging Bucket 4 place Tmax 194 8 mm 5 3 5 300 6 130 4 x 500 mL J TB 089 o 24 microplates 8 deep well plates 4 square well plates 2 b c d o ag a 50 Hz centrifuge is 14 000 rpm 4 C minimum at 13 000 rpm 35 C ambient Maximum speeds are based on a solution density of 1 2 g mL in all rotors Maximum speed for the rotor in the Avanti J E centrifuge At 35 C ambient temperature and 95 percent humidity Maximum speed for rotor without magnets is 13 000 rpm Maximum speed for rotor without magnets is 6 300 rpm Maximum speed in the Avanti J E for the rotor with magnets without magnets maximum is 14 000 rpm Maximum speed at 2 C in Temperature performance for the F14BCI 14x50cy rotor in the Avanti J E is as follows 12 C minimum at 14 000 rpm at 35 C ambient 2 Preinstallation Requirements Preinstallation requirements have been provided for your Avanti J E centrifuge The following information is included in case the instrument must be relocated After t
18. L 8 A28219 AA Note On the instrument the triangle background is yellow rather than gray This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environ ment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with this instruction manual may cause interference to radio communica tions Operation of this equipment in a residential area is likely to cause interference in which case the user will be required to correct the interference at his own expense This equipment does not exceed the Class A limits for radio noise emissions from digital apparatus as set out in the radio interference regulations of the Canadian Department of Communications Le pr sent appareil num rique n met pas de bruits radio lectriques d passant les limites applicables aux appareils num riques de Classe A prescrites dans le reglement sur le brouillage radioelectrique dict par le Minist re des Communications du Canada This symbol is required in accordance with the Waste Electrical and Electronic Equipment WEEE Directive of the European Union The presence of this marking on the product indicates 1 the device was put on the European market after August 13 200
19. OOOO 6000 2m Zzm Description The centrifuge has two acceleration profiles MAX maximum acceleration from 0 rpm to set speed or SLOW slow acceleration from 0 to 500 rpm followed by maximum acceleration to set speed The AccEL key is used to select the maximum acceleration or slow acceleration provided to maintain optimum separation The centrifuge has three deceleration profiles MAX full dynamic braking to 0 rpm SLOW brake to 500 rpm followed by reduced braking to a gentle stop OFF coast from set speed to stop The DECEL key is used to select the deceleration rate provided to main tain optimum separation A toggle switch used to enter into and exit out of the program mode to create or recall programs When the key is pressed the program number can be selected using the keypad Saves the program in memory after program run parameters have been entered The keypad is used to enter numerical run parameters to enter or recall a program number and to select acceleration and or decelera tion rates In addition to keys 0 through 9 the keypad includes plus or minus decimal key and a CE clear entry key The key is used to enter a temperature setting below 0 C for example 2 C or to enter a decimal point in a rotor entry code depending on the parameter key selected The cE clear entry key clears any parameter on the display Pressing CE will also clear some diagnostic messages If a diagno
20. Qao B COULTER Avanti J E Centrifuge Instruction Manual Symbol Symbol Symbole S mbolo Simbolo Title Titel Tire Titulo Titolo Qi ZF Dangerous voltage Gefahrliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo alta tensione EREE fi E HEL As Attention consult accompanying documents Achtung Begleitpapiere beachten Attention consulter les documents joints Atenci n consulte los documentos adjuntos Attenzione consultare le informazioni allegate GE ANA BROZ E ER WER MT AICHE On power Ein Netzverbindung Marche mise sous tension Encendido Acceso sotto tensione SiR FF EW Off power Aus Netzverbindung Arr t mise hors tension Apagado Spento fuori tensione V EM K EM Protective earth ground Schutzleiteranschlu Liaison la terre Puesta a tierra de protecci n Collegamento di protezione a terra RETA GEM PRIE Earth ground Erde Terre Tierra Scarica a terra PA EH FH SAFETY NOTICE This safety notice summarizes information basic to the safe operation of the equipment described in this manual The international symbol displayed above is a reminder that all safety instructions should be read and understood before installation operation maintenance or repair of this centrifuge When you see the symbol on other pages pay special attention to the safety information presented Observanc
21. allowed time Run shuts down with maximum brake Check and clean door sealing area and door gasket Wipe any ice and excess moisture from chamber Call Beckman Coulter service R1 and R2 Rotor ID problem No magnets identified or magnets incorrectly identified Run continues speed may be derated Press CE to clear message If problem repeats check rotor magnets or call Beckman Coulter service R3 R4 and R8 Rotor speed derated The entered rotor number is not the same as the rotor identified If identified rotor speed maximum is lower than entered maximum the speed will be reduced to the rated maximum of the installed rotor Press CE to clear message Enter the correct rotor entry code R5 and R6 No rotor match The system cannot identify the rotor Run shuts down with maximum brake Make sure the rotor in use is a compatible Beckman Coulter rotor see AVAILABLE ROTORS in Section 1 Call Beckman Coulter service R9 Calibration error Rotor calibration error Run shuts down with maximum brake Make sure the rotor in use is a compatible Beckman Coulter rotor see AVAILABLE ROTORS in Section 1 Call Beckman Coulter service Friction Reduction System Continued 4 3 Troubleshooting 4 4 Table 4 1 Diagnostic Message Chart continued Diagnostic Number Message Problem Result Recommended Action S1
22. an be unlocked only by pressing DOOR and opened only when the power is on and the rotor is at rest If there is a power failure the door lock can be manually tripped for sample recovery see Section 4 TROUBLESHOOTING PROTECTIVE RING IMBALANCE DETECTOR Description A structural steel ring surrounding the rotor chamber provides full operator protection An imbalance detector monitors the rotor during the run causing automatic shutdown if rotor loads are severely out of balance At low speeds an incorrectly loaded rotor can cause an imbalance Rotor instability can also occur if the instrument is moved or if it is not resting level on the floor see Section 4 TROUBLESHOOTING OVERSPEED AND ROTOR IDENTIFICATION SYSTEM NAME RATING PLATE A rotor identification system prevents the installed rotor from running above its maximum rated speed During acceleration the micropro cessor checks the magnetic rotor identification If the system identifies a rotor different than the one entered by the user and the set speed is above the maximum for the identified rotor the system will reduce the set speed to the maximum for the installed rotor A second check based on rotor physical properties is performed during acceleration Speed is limited to the maximum safe speed of the identified rotor group IB NOTE Some rotors manufactured before January 2002 may not have magnets installed Based on the rotor property grouping these
23. applicable regulatory agencies Declarations of conformity and certificates of compliance are available at www beckmancoulter com This manual is designed to familiarize you with the Avanti J E centrifuge its functions specifications operation and routine operator care and maintenance We recommend that you read this entire manual especially the SAFETY NOTICE and all safety related information before operating the centrifuge or performing instrument maintenance Section 1 contains system specifications and a brief physical and functional description of the centrifuge including the operating controls and indicators and available rotors Section 2 provides information about space and power require ments for installing and connecting the centrifuge Section 3 contains centrifuge operating procedures Section 4 lists possible malfunctions together with probable causes and suggested corrective actions Section 5 contains procedures for routine operator care and main tenance as well as a brief list of supplies and replacement parts vil Introduction CONVENTIONS NOTES CAUTIONS AND WARNINGS viii I gt NOTE If the centrifuge is used in a manner other than specified in this manual the safety and performance of this equipment could be impaired Further the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety Use of any equipment not specifically recomm
24. centrifuge must have adequate air ventilation to ensure compliance to local requirements for vapors produced during operation Figure 2 1 Dimensions of the Avanti J E Centrifuge Preinstallation Requirements Position the centrifuge so that the air diverter nearly touches the wall behind the centrifuge Place the power cord to one side of the air diverter To avoid damaging the power cord when installing or moving the centrifuge be sure to move the cord out of the way before pushing the centrifuge toward the wall Relative humidity should not exceed 95 noncondensing ELECTRICAL REQUIREMENTS 200 V centrifuge 180 220 VAC 24 A 50 60 Hz 230 V centrifuge 207 253 VAC 24A 50 60 Hz 208 240 V centrifuge 187 229 216 264 VAC 24A 50 Hz To reduce the risk of electrical shock this centrifuge uses a perma nently attached 1 8 m 6 ft three wire UL CSA approved electrical cord and plug to connect the centrifuge to earth ground Contact your local Beckman Coulter office for specific information regarding local plug requirements To preserve this safety feature Power to the centrifuge should originate directly from a main power line transformer at a power source known to be clear of erratic loads spikes and electromagnetic interference Make sure there are properly rated thermal circuit breakers at the wall service panel to protect the centrifuge circui
25. ch located on the right panel of the centrifuge controls electrical power to the centrifuge It is also a circuit breaker that will trip to cut off power in the event of a power overload The power switch must be turned on before the chamber door can be opened The control panel see Figure 1 2 is mounted at an angle on the centrifuge front for easy visibility and access It is used to enter run parameters via function keys and a keypad and to display run param eters program information and user messages 16 O00RPM 5 53 Es Es DE ED en C8 robe O 16250 MAX MAX YU C G3O G9 9 OOO Ooo 9 E Cosa Figure 1 2 Control Panel Digital Displays o SPEED O TIME j TEMPERATURE Aa ROTOR ACCEL DECEL Description The displays provide run information user messages and diagnostics During a run they show the actual real time operating conditions When the run parameters are being entered they show the set values selected Set values can be recalled during operation by pressing SHOW SET After 5 seconds the displays return to actual conditions Indicates rotor speed in revolutions per minute rpm or in relative centrifugal field a term describing the ratio of the centrifugal acceler ation at a specified radius and speed to the standard acceleration of gravity RCF x g na timed run indicates the remaining run time in
26. ct a An alternate method of selecting a rotor is to rotor by entry code number press ROTOR repeatedly the rotor number changes each time the key is pressed When the rotor you are using is displayed press ENTER 3 Press the next run parameter key or press ENTER 3 5 Operation ENTERING RUN SPEED Action 1 Press RPM RCF once to enter speed in RPM or twice to enter speed in RCF 2 Use the keypad to enter required speed 3 Press the next run parameter key or ENTER ENTERING RUN TIME 3 6 Enter a run speed up to the maximum speed of the rotor in use 21 000 rpm maximum Or enter a relative centrifugal field RCF value up to the maximum achievable RCF of the rotor Result Allowed speed for the selected rotor is displayed Entered speed appears on the speed display If the entered speed is higher than the rated speed of the installed rotor a message showing the valid speed range will be displayed Press CE and enter an acceptable value You can change the set speed at any time by repeating steps 1 through 3 The rotor will accelerate or decelerate to the new speed I gt NOTE During acceleration the instrument identifies the rotor and checks its maximum allowable speed If set speed is greater than the rotor group s maximum allowable speed the rotor will decelerate to its rated speed and an error message will be displayed Refer to Section 4 TROUBLESHOOTING Run time can be set fo
27. d dry by frequent wiping with a cloth or paper towel For thorough cleaning wash the chamber using a mild detergent such as Beckman Solution 555TM diluted 10 to 1 with water Rinse thoroughly and dry completely Clean the centrifuge exterior surfaces by wiping with a cloth dampened with Solution 555 diluted 10 to 1 with water Do not use acetone Maintenance Clean the drive hub regularly using Solution 555 diluted 10 to 1 with water and a soft brush Rinse thoroughly and dry completely Tube Breakage Ifa glass tube breaks and all the glass is not contained in the bucket or rotor be sure to thoroughly clean the chamber N WARNING Be careful when examining or cleaning the chamber and chamber gasket as sharp glass fragments may be embedded in their surfaces Examine the chamber gasket to make that no glass particles are retained in it Carefully remove any glass particles that may remain Carefully wipe away any glass particles that remain in the chamber DECONTAMINATION Ifthe centrifuge and or accessories are contaminated with radioactive a A 4 or pathogenic solutions perform all appropriate safety and decontam A e ination procedures Refer to Chemical Resistances publication IE amp IN 175 to be sure the decontamination method will not damage any part of the instrument STERILIZATION AND DISINFECTION The centrifuge is finished with urethane paint Ethanol 70 may be used on this surface See
28. d to enter the required program number then press ENTER Press SHOW SET to display program parameters 5 ENTER Check that all parameters are correct and that the door is closed START Press ENTER then press START within 5 seconds 6 STOP Wait for the run to end or end the run by pressing STOP 7 DOOR When the rotor stops a tone sounds press DOOR to unlock the chamber door lift the door open to remove the rotor Operation MANUAL RUN 1 POWER Turn the power switch on 2 DOOR Press DOOR to unlock the chamber door lift the door open 3 Install the rotor according to the applicable rotor manual then close the chamber door and press DOOR 4 ROTOR Press ROTOR then use the keypad to enter the rotor entry code 5 RPM RCF Press RPM RCF then use the keypad to enter the run speed 300 to 21 000 rpm Press RPM RCF a second time to enter speed in RCF 6 TIME Press TIME then use the keypad to enter the run time to 99 hrs 59 minutes or press TIME twice for a hold continuous run 7 TEMP C Press TEMP C then use the keypad to enter the required run temperature 10 to 40 C 8 ACCEL Press ACCEL then use the keypad to enter the selected accelera tion rate number 1 or 2 9 DECEL Press DECEL then use the keypad to enter the selected decelera tion rate number 1 2 or 3 10 ENTER Check that all param
29. e operation when power is restored and the rotor will return to set speed However if the rotor came to a complete stop you will have to restart the run when the power is restored In either case a power outage message will be displayed on the control panel to indicate that a power outage has occurred Troubleshooting A WARNING Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and or mechanical injury Therefore turn the power off and disconnect the instrument from the main power source and refer such mainte nance to service personnel In the event of an extended power failure it may be necessary to override the door locking mechanism manually to remove the rotor and retrieve your sample A WARNING The following procedure should be imple mented only when absolutely necessary and only by qualified service personnel Action Result 1 Turn the power off and No lights on the control panel are lit disconnect the power cord from the main power source A CAUTION 2 Insert the hex wrench LISTEN CAREFULLY Do not proceed if any 729317 or a similar tool sound or vibration is coming from the drive into the openings on each side of the centrifuge see Figure 4 1 to release the top front panel 4 5 Troubleshooting Hex Wrench Action 3 Lower the panel as far as the tether cords allow Use the latch override key 368247 to turn the
30. e of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the centrifuge Safety During Installation and or Maintenance This centrifuge is designed to be installed by a Beckman Coulter Field Service representative Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument Any servicing of this equipment that requires removal of any covers can expose parts which involve the risk of electric shock or personal injury Make sure that the power switch is off and the centrifuge is disconnected from the main power source and refer such servicing to qualified personnel Use the anchoring system to secure the centrifuge in place The anchoring system is designed to reduce the possibility of injury or damage that could result from instrument movement in the event of a major rotor mishap Do not replace any centrifuge components with parts not specified for use on this instrument Electrical Safety To reduce the risk of electrical shock this equipment uses a three wire electrical cord and plug to connect the centrifuge to earth ground To preserve this safety feature Make sure that the matching wall outlet receptacle is properly wired and earth grounded Check that the line voltage agrees with the voltage listed on the name rating plate affixed to the centrifuge Never use a three to two wire plug adapter Never use a two
31. e requirements the centrifuge must be secured to the floor using the anchoring hardware shipped with the instrument This will prevent the centrifuge from moving in the unlikely event of a rotor mishap Complete instructions for installing the anchoring kit are packaged with the hardware which is shipped with the centrifuge The instruc tions publication SJ TB 003 include a full size template to be used as a guide for drilling holes in the floor Refer to this document for additional installation instructions gt NOTE Beckman Coulter representatives are not equipped to drill holes in your floor The holes must be drilled before your scheduled installation Preinstallation Requirements BIO SAFETY LEVEL 3 INSTALLATION For laboratories with epoxy aggregate floors such as BSL 3 labs a non invasive installation kit PN 393830 is available The kit which consists of an adhesive backed mounting plate is CSA certified for use on epoxy aggregate floors only 2 5 3 Operation This section contains manual and programmed operating procedures A summary is provided at the start of this section If you are an expe rienced user of this centrifuge you can turn to the summary for a quick review of operating steps A WARNING Normal operation may involve the use of solu tions and test samples that are pathogenic toxic or radioactive Handle body fluids with care because they can transmit disease No known tes
32. ended in this manual and or the applicable rotor manual is the sole responsibility of the user Certain symbols are used in this manual to call out safety related and other important information These international symbols may also be displayed on the centrifuge and are reproduced and described below and on the inside of the front cover I gt NOTE Used to call attention to important information that should be followed during installation use or servicing of this equipment A CAUTION Used to indicate a potentially hazardous situa tion which ifnot avoided may result in minor or moderate injury and or mechanical damage lt is also used to alert against unsafe practices Introduction A WARNING Used whenever an action or condition may potentially cause personal injury or loss of life Mechanical damage may also result LN WARNING Indicates high voltage or risk of electric shock Refer servicing of all areas displaying either symbol to service personnel TYPOGRAPHIC CONVENTIONS Certain typographic conventions are used throughout this manual to distinguish names of user interface components such as keys Keynames for example START or PROGRAM appear in capital letters within brackets CFC FREE CENTRIFUGATION H To ensure minimal environmental impact no CFCs are used in the manufacture or operation of Avanti J E centrifuges Introduction RADIO INTERFERENCE CANADIAN REGULATIONS RECYCLING LABE
33. er Brackets Figure 5 1 Air Filter Replacement 5 4 Maintenance CIRCUIT BREAKER AND FUSES There are no user replaceable fuses in the centrifuge If the centrifuge circuit breaker trips for any reason the power switch will move to the OFF O position Reset the circuit breaker by turning the power switch back to the ON I position If it trips again immediately do not reset it Call Beckman Coulter Field Service A CAUTION Repeated attempts to reset the centrifuge circuit breaker can cause substantial damage to electrical and electronic components STORAGE AND TRANSPORT STORAGE To ensure that the centrifuge does not get damaged contact Beckman Coulter Field Service for specific instructions and or assistance in preparing the equipment for transport or long term storage Temperature and humidity conditions for storage should meet the environmental requirements described under SPECIFICATIONS 5 5 Maintenance 5 6 SUPPLY LIST REPLACEMENT PARTS SUPPLIES I gt NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1 800 742 2345 in the United States or by contacting your local Beckman Coulter office Contact Beckman Coulter Sales 1 800 742 2345 in the United States worldwide offices are listed on the back cover of this manual for information about ordering parts and supplies A partial list of supplies is given below for your convenience See the Beckman Coulter Hi
34. eters are correct and that the door is closed START Press ENTER then press START within 5 seconds 11 STOP Wait for the set time to count down to zero or end the run by pressing STOP 12 DOOR When the rotor stops a tone sounds press DOOR to unlock the chamber door lift the door open to remove the rotor or sample PREPARATION Prepare the rotor for centrifugation as described in the applicable rotor manual For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration 3 3 Operation 3 4 INSTALLING THE ROTOR Action 1 Turn the power switch on 2 Press DOOR to unlock the door 3 Lift the door up to open 4 Install the rotor according to directions in the rotor manual Ensure that the rotor is seated on the drive hub Avoid bumping the control panel keys during rotor installation or removal If you bump the control panel and an L1 diagnostic appears press CE to clear the message The power must be turned on before you can unlock and open the chamber door To end a run for any reason do not turn the power switch off press STOP instead Result Indicator lights on the control panel light up The instrument will accept this command only when the rotor is at rest A CAUTION Rotors used in Avanti J series centrifuges must have drive pins in the rotor drive hole These drive pins engage with the centrifuge drive hub to ensure that t
35. gh Performance High Speed High Capacity Rotors Tubes amp Accessories catalog BR 8102 available at www beckman coulter com for detailed information on ordering rotors tubes and accessories Alis AAA AD RAS 978347 Latch override key comico id 368247 Memory flash card kit 369173 Spinkote lubricant 2 0Z 306812 Silicone vacuum grease 1 OZ 335148 Beckman Solution 555 1 qt 339555 Hex key panel removal 729317 Program library record 233679 AVANTI J E CENTRIFUGE WARRANTY Subject to the exceptions and upon the conditions specified below Beckman Coulter agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within one 1 year 2 years for the drive motor after delivery of the Avanti J E Centrifuge centrifuge the product to the original buyer by Beckman Coulter or by an authorized representative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use Some components and accessories by their nature are not intended to and will not function for as long as one 1 year If any such component or accessory fails to give reasonable ser vice for a reasonable period of time Bec
36. he centrifuge is moved it must be leveled by adjusting the two front feet I gt NOTE This centrifuge is designed to be installed by a Beckman Coulter Field Service representative Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument SPACE REQUIREMENTS A WARNING Do not place the centrifuge near areas containing flammable reagents or combus tible fluids Vapors from these materials could enter the centrifuge air system and be ignited by the motor Maintain a 30 cm 1 ft clearance area around the centrifuge while itis running No persons or any hazardous materials should be within this area while the centrifuge is operating except to change operating controls if required 2 1 Preinstallation Requirements 2 2 If it is necessary to move the centrifuge maintain the following conditions Select a location away from heat producing laboratory equipment with sufficient ventilation to allow for heat dissipation Thecentrifuge must have adequate air ventilation to ensure compliance to local requirements for vapors produced during centrifuge operation Position the centrifuge on a level floor that can support the weight of the centrifuge and resist vibration naddition to space for the instrument itself allow a 7 7 cm 3 in clearance on each side of the instrument and a 16 cm 6 25 in clearance behind the instrument for air circulation The
37. he rotor does not slip during acceleration Some Beckman Coulter rotors were originally manufactured without drive pins because these rotors did not need pins to run in older model centrifuges To check for drive pins hold the rotor up or turn it on its side and look into the drive hole If you do not see at least two metal pins near the top of the hole do not use the rotor in the Avanti J E ECKMA yy Drive Pins Angled pins shown Pins can also be vertical or horizontal Drive Spindle Assembly Operation Action Result 5 Close the chamber door and The door latches When the latches engage the LED next to the press DOOR To keep the DOOR key lights chamber clean and dry leave the door closed whenever possible MANUAL OPERATION When a run parameter key ROTOR RPM RCF TIME TEMP ACCEL or DECEL is pressed a parameter can be entered or changed Entry is completed when ENTER or another function key is pressed To change an entry before you ve pressed ENTER or another parameter key press CE and enter a different value To change an entry after you ve pressed ENTER press the run parameter key again e Ifan unacceptable value is entered the valid range for that param eter is displayed in the message line Enter the correct value SELECTING A ROTOR Action Result 1 Press ROTOR A list of rotors with rotor entry codes scrolls across the display gt NOTE 2 Use the keypad to sele
38. kman Coulter will repair or at its election replace such component or accessory What constitutes either reasonable service and a reasonable period of time shall be determined solely by Beckman Coulter REPLACEMENT Any product claimed to be defective must 1f requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer with the trans portation charges collect unless the product is found to be defective in which case Beckman Coulter will pay all trans portation charges Beckman Coulter makes no warranty concerning products or accessories not manufactured by it In the event of failure of any such product or accessory Beckman Coulter will give reasonable assistance to the Buyer in obtaining from the respective manufacturer whatever adjustment is reasonable in light of the manufacturer s own warranty CONDITIONS Beckman Coulter shall be released from all obligations under all warranties either expressed or implied if the product covered hereby is repaired or modified by persons other than its own authorized service personnel unless such repair by others is made with the written consent of Beckman Coulter or unless such repair in the sole opinion of Beckman Coulter is minor or unless such modifications is merely the installa tion of a new Beckman Coulter plug in component for such product DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR RANTY SHALL BE IN LIEU O
39. nd or mechanical injury Therefore turn the power off and disconnect the instrument from the main power source and refer such mainte nance to service personnel 5 1 Maintenance MAINTENANCE CLEANING Rotor Cleaning Kit 339558 Perform the following procedures regularly to ensure continued performance and long service life of the centrifuge Inspect the centrifuge chamber for accumulations of sample dust or glass particles from broken sample tubes Clean as required see CLEANING below Check the air filter on the back panel for obstructions Keep vents clear and clean Wipe condensation out of the rotor chamber between runs with a sponge or clean cloth to prevent chamber icing f chamber icing occurs defrost the system and wipe moisture out of the chamber before use To defrost the system set the tempera ture to 30 C for 20 minutes These are suggested settings which may be adjusted as appropriate for your laboratory conditions gt NOTE Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment Clean the centrifuge frequently Always clean up spills when they occur to prevent corrosives or contaminants from drying on compo nent surfaces To prevent accumulations of sample dust and or glass particles from broken sample tubes keep the chamber clean an
40. ntrifuge Instruction Manual O 2009 Beckman Coulter Inc Contents Page INTRODUCTION Certifications ii a di aha vil Scope of Manual ai eel I vil CONVENTIONS cd Bea ea es e E ER UN viii Notes Cautions and Warnings vili Typographic Conventions ix CFC Free Centrifugation ix Radio Interference 2 x Canadian Regulations x Recycling Label vs u SE en ea REO x SECTION 1 DESCRIPTION Descriptions eoero a a Were iR AD E 1 1 Centrifuge Function 1 1 Housing and Door 1 2 Rotor Chamber 1 3 VaCuuin Sr TU C EDSITUCPN ata UU ES 1 3 Temperature Control 1 3 DIN tas kat IR dad dea 1 4 Control and Indicators 1 4 Power Switch s 2 4 nat at eS ES 1 4 Control Panel ic oe e pte RA IRURE Se RES 1 4 Safety Features io osi di ne sd ete e Sect na 1 8 DOOL ete AA AA 1 8 Protective RIng 5 ccu vec e o een eus 1 8 Imbalance Detector 1 9 Overspeed and Rotor Identification System 1 9 Name Rating Plate vita 022 8 a 2 re 1 9 Specifications ud ver RE eus dee ei EL Pose WES Set 1 1
41. operating situation when the diagnostic condition occurred such as rotor in use speed or load type and any unusual environmental and or operating conditions such as ambient temperature or voltage fluctuations Table 4 1 Diagnostic Message Chart Diagnostic Number Message Problem Result Recommended Action P1 Power failure occurred see manual Momentary power failure rotor does not come to a complete stop Run continues when power resumes Press CE to clear message P2 Power failure see manual Power failure rotor speed drops to lt 500 rpm Run restarts automat ically when power resumes Press CE to clear message L1 L2 L5 L6 L11 and L12 Reclose door Latches are not operating properly Press down on the door and press DOOR If you close the door repeatedly and the problem continues gently clean the latch area with a lintless swab Be careful not to damage sensitive electronics in the area Error message appears run shuts down with maximum brake WARNING Do not put your fingers into the latch openings Press CE to clear message C1 Rotor temp exceeds 4C above set Rotor temperature exceeds tempera ture setting by more than 4 C but less than 8 C Run continues Press CE to clear message C2 Rotor temp exceeds 8C above set Rotor temperature exceeds tempera ture setting by more than 8 C Run shuts down with Call Beckman
42. r up to 99 hours and 59 minutes or for a hold continuous run The time display begins counting down when the rotor starts to spin For a timed run the run automatically terminates when the set time reaches zero a tone sounds when the rotor has stopped For a hold run the elapsed time is displayed and the run continues until STOP is pressed Action 1 Press TIME 2 Use the keypad to enter required time or Press TIME again for a hold run 3 Press the next run parameter key or ENTER Operation Result The cursor appears Entered time appears on the display If you entered a number higher than 59 minutes the centrifuge automatically recalculates the time in hours and minutes HOLD appears on the display ENTERING RUN TEMPERATURE Action 1 Press TEMP 2 Use the keypad to enter required temperature 3 Press the next run parameter key or ENTER Run temperature can be set from 10 to 40 C If no value is entered the centrifuge selects the last entered temperature Result The cursor and the temperature range for the selected rotor appear Entered temperature appears on the display If the entered tempera ture is lower than can be achieved for the installed rotor a message showing Input error temp will be displayed 3 7 Operation 3 8 ENTERING ACCELERATION AND DECELERATION RATES Action 1 Press ACCEL 2 Press the keypad number for the required accelera
43. record 233679 The run starts NN USER MESSAGES 4 Troubleshooting This section lists possible malfunctions together with probable causes and corrective actions Maintenance procedures are given in Section 5 For any problems not covered here contact Beckman Coulter Field Service 1 800 742 2345 in the United States worldwide offices are listed on the back cover of this manual for assistance I gt NOTE It is your responsibility to decontaminate the instrument as well as any rotors and or accessories before requesting service by Beckman Coulter Field Service User messages appear on the display to communicate information about the instrument or to alert you to abnormal conditions that need attention Help and informational messages caused by incorrect input or certain operating conditions can be cleared by pressing CE and following the instructions in the message Diagnostic messages that result from abnormal operating condi tions or equipment malfunction require troubleshooting Refer to Table 4 1 to determine the nature of the condition and any recom mended actions If a problem persists after you have performed the recommended action call Beckman Coulter Field Service To help 4 1 Troubleshooting 4 2 the field service representative diagnose and correct the problem try to gather as much inform about the situation as you can including the diagnostic number and message the
44. right hand latch bolt to the right clockwise and the left hand latch bolt to the left counter clockwise until the latches release After removing the rotor replace the top front panel by pushing it into place Hex Wrench ES Latch Override Figure 4 1 Manual Door Release Result A CAUTION Do not attempt to remove tether cords They are required to assure safe operation of the centrifuge If the rotor is still spinning close the door and turn the latches to secure 1t Wait until the rotor stops spinning to repeat this step A WARNING NEVER try to slow or stop the rotor by hand INSTRUMENT CARE 5 Maintenance This section contains care and maintenance procedures that should be performed regularly For maintenance not covered in this manual contact Beckman Coulter Field Service 1 800 742 2345 in the United States for assistance User messages and recommended actions are discussed in Section 4 TROUBLESHOOTING Refer to the applicable rotor manual and Rotors and Tubes for J Series Centri fuges publication JR IM for instructions on the care of rotors and their accessories gt NOTE It is your responsibility to decontaminate the instrument as well as any rotors and or accessories before requesting service by Beckman Coulter Field Service A WARNING Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock a
45. ring centrifugation the number of the program being run is displayed Run parameter keys are used to enter specific run information as follows Pressed to display scrolling list of compatible rotors and rotor entry codes Can be pressed repeatedly to scroll more quickly through the rotor list Ifthe display shows speed in rpm the key is pressed once to enter run speed using the keypad in rpm revolutions per minute pressed twice to enter run speed in RCF relative centrifugal field If the display shows speed in RCF the key is pressed once to enter speed in RCF or twice to enter speed in rpm Pressed to enter run time using the keypad When TIME is pressed twice HOLD mode is selected Pressing it again reverts to time entry mode Timed run Run time up to 99 hours and 59 minutes can be set If more than 59 minutes are entered in the minutes field the system automatically converts the entry to hours and minutes Decelera tion begins when the set time counts down to zero e Continuous run hold For runs of unspecified lengths hold mode is used When 99 hours 59 minutes is reached the system resets to 0 and continues counting A hold run continues until the STOP key is pressed Pressed to enter run temperature using the keypad from 10 to 40 C If a new temperature is not entered the centrifuge uses the temperature set for the previous run is ACCEL DECEL Y PROGRAM E fo m OOOO
46. rosol containment The friction reduction system FRS uses a mechanical rotary vane vacuum pump to reduce chamber pressure to approximately one half atmosphere The pump turns on when the run is started before rotor friction reaches a high level Vacuum in the chamber is vented auto matically during rotor deceleration The temperature control system cools by circulation of a non CFC based refrigerant The system is activated when the centrifuge power is on and when the door is closed and latched A thermistor in the rotor chamber continuously monitors the chamber temperature The system calculates the chamber temperature required to maintain the set rotor temperature 2 C Although the chamber temperature fluctuates during operation the rotor s large mass keeps the sample temperature substantially constant At the end of a run the system continues controlling the temperature for 30 minutes to prevent freezing or overheating of the sample gt NOTE The system will always shut down using maximum brake if the temperature goes above 50 C 1 3 Description 1 4 DRIVE The drive shaft is belt driven by a brushless high torque switched reluctance motor The instrument s resilient suspension minimizes disturbance of the sample during acceleration and deceleration and reduces damage to the drive shaft if an imbalance occurs during centrifugation CONTROLS AND INDICATORS POWER SWITCH CONTROL PANEL The power swit
47. rotors may be limited to a lower speed than the rated speed A name rating plate is affixed to the rear of the instrument Always mention the serial number and model number when contacting Beckman Coulter regarding your Avanti J E centrifuge 1 9 Description SPECIFICATIONS Only values with tolerances or limits are guaranteed data Values without tolerances are informative data without guarantee Speed Set speed 300 to 21 000 rpm in 10 rpm increments Speed control actual rotor speed 50 rpm of set speed Speed display actual rotor speed in 10 rpm increments or actual ref x g Time Sette rien nn een to 99 hours 59 minutes or continuous hold Time display Timed run indicates run time remaining Hold r um essen ass EUER displays elapsed time Temperature Set temperature 10 to 40 C in 1 increments Temperature control after equilibration 2 C of set temperature Ambient temperature range 15 to 35 C Humidity restrictions lt 95 noncondensing Vaci anregen ee 1 2 atmosphere Acceleration two profiles MAX full acceleration to set speed and SLOW Deceleration three profiles MAX SLOW and OFF coast Dimensions WI e der VS GERE ge en 63 5 cm 25 in Depths tenn a a do d s eS Met 80 0 cm 31 5 in Height door closed 91 4 cm 36 in
48. stic message does not clear when CE is pressed see Section 4 TROUBLESHOOTING The ENTER key can be pressed to save parameter inputs to system memory during manual run setup and to start the centrifuge ENTER then START must be pressed to start the centrifuge 1 7 Description 1 8 SHOW SET a a START STOP in SAFETY FEATURES DOOR Pressed during a run to display set values for 5 seconds Pressing ENTER then START begins the run START must be pressed within 5 seconds of pressing ENTER This key combination can also be used to abort a deceleration process and restart the centrifuge Can be pressed at any time while the rotor is spinning to terminate arun The centrifuge decelerates to a complete stop according to the deceleration setting The green light next to the key flashes while the rotor decelerates An audio indicator sounds when the rotor comes to a complete stop Locks and unlocks the door The instrument will not accept the open command if the rotor is spinning The Avanti J E centrifuge has been designed and tested to operate safely indoors at altitudes up to 2 000 m 6 562 ft Safety features include the following The steel and structural foam door has an electromechanical door locking mechanism with dual latches to prevent operator contact with a spinning rotor When the door is closed and DOOR is pressed the latches automatically engage Door LED will come on It c
49. t If fuses must be used instead of the specified circuit breakers the fuses may require a rating of greater than 30 amperes Figure 2 2 shows the power connection 30 ampere Circuit Breaker Wall Outlet Hubbell 9330 Bryant 96 30 FR or Equivalent NEMA 6 30 R Sa Measured Line Earth Voltage Ground 30 ampere Circuit Breaker Y Figure 2 2 Single Phase Power Connection 2 3 Preinstallation Requirements A WARNING To reduce the risk of electrical shock this equipment uses a three wire or five wire elec trical cord and plug to connect the centrifuge to earth ground To preserve this safety feature make sure that the matching wall outlet receptacle is properly wired and earth grounded Check that the line voltage agrees with the voltage listed on the name rating rating plate affixed to the centrifuge Never use a three to two wire plug adapter Never use a two wire extension cord or a two wire non grounding type of multiple outlet receptacle strip e fthere is any question about voltage have a qualified service person measure it under load while the drive is operating To ensure safety the centrifuge should be wired to a remote emer gency switch in order to disconnect the centrifuge from the main power source in case of a malfunction SECURING THE CENTRIFUGE TO THE FLOOR 2 4 Avanti J series centrifuges are certified to meet the requirements of the European CE mark To meet thes
50. t offers complete assurance that they are free of micro organisms Some of the most virulent Hepatitis B and C and HIV I V viruses atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other infectious samples according to good labora tory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this centrifuge without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection 3 1 Operation A WARNING Do not use the centrifuge in the vicinity of flammable liquids or vapors and do not run such materials in the instrument Do not lean on the instrument or place items on it while it is operating SUMMARY OF RUN PROCEDURES For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration PROGRAMMED RUN 1 POWER Turn the power switch on 2 DOOR Press DOOR to unlock the chamber door lift the door open 3 Install the rotor according to the applicable rotor manual then close the chamber door and press DOOR 4 PROGRAM Press PROGRAM use the keypa
51. tion rate 1 MAX 2 SLOW 3 Press the next run parameter key or ENTER 4 Press DECEL 5 Press the keypad number for the required deceleration rate MAX 2 SLOW 3 OFF The instrument provides a choice of two acceleration rates and three deceleration rates to protect the gradient and sample to gradient inter face The acceleration time is the time it takes a rotor to reach set speed from rest The deceleration time is the time it takes a rotor to decelerate from set speed to rest Result Acceleration options are displayed Entered acceleration rate appears on the display Deceleration options are displayed Entered deceleration rate appears on the display STARTING A RUN Action 1 Press ENTER and START 2 After the rotor has stopped press DOOR 3 Remove the rotor Keep the chamber door closed between runs Operation Result The green light next to the START key flashes and the rotor begins to spin I gt NOTE To begin a run ENTER must always be the last key pressed before pressing START If you wait more than 5 seconds the START key will not activate If this happens press ENTER and START again to begin the run If an unacceptable value was entered the run will not begin when ENTER and START are pressed Check the display and make any necessary corrections or additions Press ENTER and START again to begin the run The run will end when the
52. xt to the sroP key will flash while the rotor decelerates A tone will sound when the rotor has come to a complete stop The door is unlocked RECALLING AND CHANGING A PROGRAM Recalling a Program A program that has been saved in memory can be recalled at any time Operation Action 1 Press PROGRAM 2 Selectthe appropriate keypad number and press ENTER 3 Press SHOW SET to display program parameters 4 To begin the run press ENTER and START Changing a Program Action 1 Press PROGRAM 2 Selectthe appropriate keypad number and press ENTER 3 Press SHOW SET to display program parameters 4 Press the run parameter key to be changed 5 Use the keypad to enter the new value 6 Repeat steps 4 and 5 to change other run parameters When all changes have been entered press SAVE 7 Press ENTER and START to begin the programmed run or press PROGRAM to exit program mode and return to manual operation Result Previously saved program numbers in use are displayed The message Press ENTER START to run or edit parameters 1s displayed The run starts Result Previously saved program numbers are displayed The message Press ENTER START to run or edit parameters 1s displayed Parameter prompts are displayed The revised program will remain in memory until further changes are made and saved Record the changes on your Program Library
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