Catalog - MicroData Instrument, Inc.
Contents
1. Basic Accessories IP 1 Input tubing connector 6 for PM6000 10 for PM8000 OP 2 output tubing connector 4 for PM6000 8 for PM8000 OV 1 5 perfusion vial 1 5ml 4 for PM6000 8 for PM8000 HOLD 4 7 4 7 barrel pipette holder PP 4 7 pulled 4 barrel pipettefor PM6000 7 barrel for PM8000 FSW foot switch Power cord User s manual Optional Accessories OP 4 5ml OP 10ml OP 30ml perfusion drain vial Tested Cell STB 1 5 magnetic stand and 1 5ml vial bracket HOLD 1 single pipette holder PP 4 PP 7 pulled multibarrel pipette MOUSE 2 special remote mouse PM KP remote key pad RK 2 rack mounted kit with handle WEG WEG Ko NEP NEL Kat ELE NEL OP 4 5ml or OP 30ml Perfusion DT 800 Digital Tope Recorder Vials for Testing Drugs or Agents Signal Conditioner or OO O Patch Clamp Amplifier Seil 0100 ooo0o002020 09oocoooogo 2L l Cl Eed L 1 Trigger Signal SPECIFICATIONS Output Channels 4 PM6000 or 8 PM8000 injection perfusion outputs one synchronized drain out and one cell hold channel2. B Products and Applications me forthe Life Science Laboratory Microinjectors Microperjusion Systems Multi Channel Microfluidic Pump Multi barrel Micropipette Pullers Single Barrel Micropipette Pullers Quartz Micropipette Puller Microforge Grinding Centers Inverted Microscope Work station A A Contents Cell Microinjectors 1 Multichannel Perfusion Injection Systems 3 Programmable Multichannel Microfluidic Pump 5 Multipipette Pullers 7 Micropipette Pullers 9 Quartz Micropipette Puller 11 Microforge Grinding Centers 13 Inverted Microscope with mounting brackets 17 Accessories for PM Series 18 en Dm MicroData Instrument Inc 1207 Hogan Drive South Plainfield NJ 07080 U S A Tel 908 222 1717 Fax 908 222 1365 e mail info microdatamdi com http www microdatamdi com CAT 11 14 Ordering Information How to Order Telephone orders must be confirmed in writing by fax or mail All purchase orders must be signed by an authorized purchasing agent or person Export Order Orders outside of the U S A and Canada should be placed directly to this office or to the local rep resentatives Payment of U S A currency must accompany all direct orders Contact our sales department for quotation of shipping costs and pay ment
3. 0 1 psi for pressure output TIMERSETTINGRANGE 0 01sec to 327 66 sec WORKING TEMPERATURE AC 43C 39F 105F 0 01sec resolution for COMPUTERINTERFACE DB9 connector with techni whole range to EX cal code reference op TEND or 0 MANU tional control SAVABLESEQUENCES 15 Programmable sequences MICROFLUIDIC SYSTEM SETUP DIAGRAM PMFP Programmable Multichannel Microfluidic Pump Digital Recorder O0 Lao ED ei aer N 666666606 EEF oogogooof Pressure Source 66660666 KE EE Transducer Signal Trigger Signal Amplifier A input Transducer CS i ODOD Oe FSH Foot Switch PM KP Foot Switc 4 HHH Remote Key Pad Optional Devices TA Optional Accessory LAYS E y gw ug z D Microfluidic Flow Cell or Ka Microfluidic Chip Latz EA UO E OE M D dD dD d E d D ce OP 4 5ml or e 30ml Perfusio Vials for Testing Drugs or Agents Optional Accessory PMP 107 Programmable Multipipette Puller em ED les Ma prima aaeeea bm PMP107 Multipipette Puller Um PERRELS FEIRES FRE LPHEE A N 2 j 3 3 d a 1 am
4. Input Gas Pressure 30 100 psi Clearing Pressure Same as input pressure Clear Hold Pressure 0 3 psi regulated Injection Pressure 0 05 60 psi regulated Balance Pressure 0 05 10 psi regulated Fill Vacuum 0 24 Hg regulated Hold Vacuum 0 30 of water regulated Drain Vacuum 0 30 of water regulated Repeatability 0 05 psi Display Accuracy 0 1 Timer Setting Range 10 msec 327 67 sec 10 msec Resolution for whole range Counter Setting Range 255 Savable Sequences 16 sequences Programmable Steps 32 steps for each sequence Remote Control Ports DB9 connector forremote key pad foot switch jack or BNC for nega tive TTL trigger a jack connector for injection foot switch Signal Output Ports 4 PM6000 or8 PM8000 BNCs for injection signals Power Consumption 100 1200r220 VAC 35 Watts MicroData Instrument Inc http www microdatamdi com e mail info microdatamdi com PMFP Programmable Multichannel Newt Microfluidic Pump i CC Y OS SZ SES P D H KEY FEATURES Multichannel pressure outputs with versatile capabilities and compact size for microfluidic solution driving e Up to eight independent pressure output channels plus one adjustable vacuum suction output channel e Programmable and savable timers and automatic multistep sequences Adjustable nine output pressures one suction vacuum and savable readings e Nine independent synchronize signal outpu
5. for one to seven barrel pipette and 0 180 degree bevelling Up to 800 x magnification in long working dis tance objective and wide field eyepiece Scale eyepiece for precision measurement Adjustable precision power supply for variable grinder speed and heating level High power background and side LED lights for better im age Foot switches controllable Syringe and special tubing for wed grinding Optional pressure air switchable for pipette tip clearing expanding and cooling MicroData Instrument Inc Tel 908 222 1717 Fax 905 222 1365 13 The MFG Microforge Grinding Center series are a creation design for micropipette multipipette tip total modification It combines a precision microforge and a microgrinder in a hori zontal microscope compact platform It can be used for mi cropipette tip polishing shaping tipping bending breaking bev elling and grinding It is an ideal micropipette multipipette tip modify center The platform of MFG 5 Microforge Grinding Center is made from a high power horizontal binocular microscope A preci sion optical focus system with 2 axial micromanipulation pi pette holder can handle a single pipette or a multipipette con veniently and precisely The pipette holders is 180 degree turn able for different angle bevelling pipette bending or polishing Another 2 axial micromanipulator controls combination of a platinum mini heater and a microgrinder They are side by side together Turn
6. mechanics body and very reason able price make it becomes an ideal microbiology research working Specifications 100x 400x For digital photo video camera attachment Wide field eyepieces 10x F 25mm D 18mm Long working distance plan achromatic objectives cover glass thick 1 2mm 10x NA0 25WD8 1mm 20x NA0 4WD4 8mm 40x NA0 6WD3 3mm Long working distance plan achromatic phase con trast objective 10x NA0 25 WD8 1 include 10x annular diaphragm plate Long working distance condenser NA 0 4 working distance 28mm Size 200x 152mm Movement range 20x60mm Coaxial coarse and fine focusing mechanism with tension adjustable and focus stop minimum increment is 0 002mm 6V20W halogen lamp adjustable brightness Right hand side and left hand side three dimansions movement stages with mounting rods dia 10mm and brackets for micromanipulators or other micro tool connection 3 dimensional fine adjusting micromanipulator For digital camera attachment For Canon or Nikon digital camera connection and convert optics 2 5x 4x change over photo attachment include 10x viewing eyepiece 4x focusing photo attachment for Pentex 35mm camera body Wide field 16x 20x Long working distance plan achromatic phase contrast objectives 25x 0 4 40x 0 6 include 25x 40x annular diaphragm plate e mail info microdatamdi com 17 18 Accessories for PM Series 2 WT Ge MOUSE 1 an MOUSE 2 Accessories Descri
7. puller PMP 102Q Specifications Single barrel thin wall 0 1 Pre pull pressure 0 1 60 psi adjustable 1 5mm O D 0 7 1 1mm I D Mid pull pressure 0 1 60 psi adjustable Fused quartz fused silica End pull pressure 0 1 60 psi adjustable glass capillary tubing Heat Pull control Microcontrollor Pulled tapper length 0 5 10 mm Actions Start Pre pull Mid Sequences 99 programmable sequences pull End pull End Steps 18 steps per sequence pull Stop Heat Return Heater Micro torch flame and Stop Heater gas source UN2037 butane gas car Display 20x4 LCD tridge Power input 110 240 VAC Pulling force Pneumatic force Power consumption 30 watts Input airsource 60 80 psi pressure air Dimension 18L x 12W x 11H inch Gas pressure 0 1 10 psi adjustable Weight 30 lb www microdatamdi com info microdatamdi com Tel 908 222 1717 Fax 908 222 1365 MFG 5 Microforge Grinding Center 9 i a Microforge Grinding Center MFG 5 Microforge Grinding Center MFG 5A Glass micropipette tip polishing shaping tip ping bending bevelling and grinding all in one compact platform Combine platinum heater and precision microgrinder as convenient center tool on a horizontal binocular or monocular microscope platform Precise and convenient movement controls for heater grinder and pipette locations and opti cal focus Change forge to grinder or two step bevelling just in seconds Universal pipette holder
8. ringe with special connector tubing Power Supply 30W 120 240VAC e mail info microdatamdi com MFG Microforge Grinding Center Specifications Body Eyepiece Objective lenses Light Pipette movement Heater grinder movement Pipette holder Heater Microgrinder Air in output Accessory Power Supply MicroData Instrument Inc Horizontal Binocular microscope 10x 20xWE Optional 10x Scaled 4x 10x 0 25 Optional 20x 40x 0 6 Long Working Distance Two super bright white LEDs with adjustable back ground light Two axial manipulator and coarse fine focusing adjustment Two axial manipulator with three dimensional adjustment TurnableEasy clamp for single or multipipette holding Platinum wire heater combine of 0 1 0 5 um grinding disks with pre cise micromotor direct driving N A One foot switch Syringe with spe cial connector tubing 30W 120 240VAC vd a BZ http www microdatamdi com Horizontal Binocular microscope 10x 20xWE Optional 10x Scaled 4x 10x 0 25 Optional 20x 40x 0 6 Long Working Distance Two super bright white LEDs with ad justable back ground light Two axial manipulator and coarse fine fo cusing adjustment Precision manipulators with three dimen sional adjustment Turnable Easy clamp for single or multipipette holding Precision platinum wire heater 0 1 0 5 1 um grinding disks with precise micromotor direct driving Pre
9. the front panel one for a cell injection or suction pipette and another fora cell holding pipette The injection port can do clearing injecting sucking venting and balancing capillary action for the injection pipette The cell holding port can do gentle suction for holding acell Besides cell releasing function a smart proportional and adjustable pressure can be produced to push a cell out off the tip gently or clear the holding pipette completely There is savable and programmable injection timer in both PM2000 and PM1000 which can be pro grammed from 10 millisecond to continue or manual control time Besides injection timer there are sav able timers and counters for clear clear hold and fill functions inthe PM2000 There are smart Three Pulses then Continue clearandclear hold function for the PM1000 manual control All pressures are adjustable and their readings are savable for the PM2000 While in the PM1000 injection balance and hold pressures are adjustable Both microinjectors perform real time data acquisition for pressure monitoring For the PM2000 s pres sure mode the suction vacuum generating pres sure injection pressure balance pressure and hold vacuum will be displayed simultaneously as well as previous saved pressures on one LCD while the PM1000 can display balance injection and hold pressures in same time Both are convenience for user to adjust and compare pressure settings Monitor the input pressure
10. 0 1 60 psi regulated 0 10 psi regulated 14 24 Hg not regulated 0 25 of H2O regulated 0 01 to 327 67 sec 10 msec resolution CONTINUE or MANUAL control No No 1 for injection Remote mouse switches No connector One IP 1 6 input tubing with connector OPTIONAL ACCESSORIES Two OP 2 3 output tubing with connector One FSW foot switch MOUSE 2 mouse HOLD 1 HOLD 1 2 HOLD 1 5 1 1 2 or 1 5 mm O D pipette holder RK 1 rack mounting kits with handles Two OP 2 3 output tubing with connector One FSW foot switch MOUSE 1 mouse HOLD 1 HOLD 1 2 HOLD 1 5 1 1 2 or 1 5 mm O D pipette holder RK 1 rack mounting kits with handles PM KP remote control key pad Cell Microinjection Work Station Set U BL D oO Micromanipulators s and Microscope E A 2 MicroData Instrument Inc http www microdatamdi com Foot Switch e mail info microdatamdi com Programmable Multi Channel Pressure Injection Systems en MA is ion tm PMBO00 Programmable Multichannel Pressure Injector The PM6000 and PM 8000 Programmable 4 and 8 Channel Pres sure Injector systems are designed for single to eight channel intracellular injection and extracellular perfusion They are excel lent microinjection and perfusion engines for pharmacological drug testing molecular biological DNA RNA transferring intra cytoplasmic sperm in
11. 1365 SUVA y IMS 02 INVERTED MICROSCOPE WORK STATION station Magnification Trinocular Eyepieces Objectives IMS 02 WITH MICROMANIPULATORS AND PIPETTE HOLDER Condenser Object Stage Focusing Adjust Illumination Side Tool Stages OPTIONAL ACCESSORIES Manipulator C mount Adaptor D Camera Adaptor Photo Adaptor Eyepieces Objective Condenser IMS 02 WITH MICROMANIPULATORS AND PIPETTE HOLDER AND DIGITAL CAMERA MicroData Instrument Inc http www microdatamdi com Inverted Microscope with Two Side Three Dimensions Moving Stages The IMS 02 Inverted Microscope Work Station is furnished with two side three dimensions moving stages and bracket adaptors for micro manipulators in a precision inverted microscope platform The IMS 02 comes with long working distance plan achromatic objective long or ultra long working distance condenser wide field eyepiece and phase contrast attachment and C mounted adaptor for a video camera or a digital camera connection Optional digital camera connecting adap tors can be selected to fit different brand digital camera The IMS 02 is Suitable for cell operating manipulating injecting patch clamp ICSI or research of cell culture living specimen structure microbe liquid deposits microperfusion and so on It also can be used in medical treat ment research agriculture and animal husbandry research etc Its high quality optics simple but convenient
12. 2000B and PM8000 STB 1 5 STB 4 5 STB 10 STB 30 PM KP Fullfunction remote key pad for PM2000 PM2000B or PM8000 RK 1 Rack mounting kits with handles for PM 1000 or PM2000 RK 2 Rack mounting kits with handles for PM2000B or PM8000 Optional pressure air source for PM or PMP series Rolair JC10 Compressor Noise level 60 db very quiet Horsepower 1 HP 115VAC CFM Displaced 90 PSI 5 3 CFM CFM Delivered 90 PST 2 3 CFM Pump Motor RPM 1 725 RPM s Overload Protection Yes Automatic Lubrication Type Oil Less Reed Valve System Yes Standard Number of Cylinders 2 Tank Capacity 2 5 Gallons Length 17 Inches Width 16 Inches Height 15 Inches Shipping Weight 39 Pounds MicroData Instrument Inc http www microdatamdi com e mail info microdatamdi com PMP 102 Programmable Micropipette Puller UO KE Q zo 0 0 g NoU b O e D E D He e DU _ ao OH D oc m P GER da OD o 0 CL oDeg S9lJOSSO22V OV L5 CONNECTION Lu o 1207 Hogan Drive South Plainfield NJ07080 U S A Tel 908 222 1717 Fax 908 222 1365 info microdatamdi com www microdatamdi com PP 47 CONNECTION 8 e OP 4 5 CONNECTION
13. P107L e PMP102L Super Length Programmable Micropipette Puller The PMP102 is a state of the art microprocessor controlled pipette puller The PMP102 is designed to pull a pipette horizontally to produce two identical pulled micropipettes Different kinds of pipettes can be pulled repeatedly by the preset program sequences There are varied micropi pettes can be produced such as patch clamp electrodes intracellular electrodes injection micropipette and microneedles by manufactory pre set sequences To achieve these versatility and the higher degree of reproducibility many advanced technologies are applied on the PMP102 Exclusive Optical Digital Taper Measurement Instead of mechanical tip length setting from other pipette puller there is an exclusive optical digital ruler in the PMP102 to perform precise taper length setting real time measurement and controlling With this feature a user can handle taper pulling very precisely and easily Equipped with a powerful computerize tip sensing function the puller can automati cally finish the tip pulling It gives the user a quick reference to pull an ideal tip Computerize Real Time Feedback Heater Control Inside the PMP 102 there is an advanced microcontroller to perform real time heater monitoring and controlling If a heating level is selected and preset the microcontroller will measure the actual heating power during heating power on The measurement is real time displayed and feedback
14. PRESSURE INJECTION PRESSURE BALANCEPRESSURE FILLVACUUM HOLD VACUUM TIMERSETTING RANGE TINUE or MANUAL control COUNTERSETTINGRANGE 1 255 SAVABLESEQUENCE STEP FOOT SWITCH PORT REMOTE CONTROL PORT OUTPUT SIGNAL PORTS ACCESSORIES One IP 1 6 input tubing with 0 01 to 327 67 sec 10 msec resolution CON 63 sequences 12 steps for each sequence 2 for injection and sequence trigger Remote mouse DB9 interface input trigger BNC 2 BNCs for inject and fill signal output which can be used to control injection in both PM2000 and PM1000 There is another foot switch connector for triggering sequence steps on the PM2000 front panel The remote port of a DB9 connector can be connected to a remote mouse switches an optional remote keypad PM2000 nearby the micromanipu lators to forma very convenient microinjection work station The remote control port can be connected as acomputer interface port for cell electrophysiology applications PM2000 Furthermore there are BNC connectors in the PM2000 for the timing injection suction and sequence step signal in and out to synchronize other electrophysiological instruments While the PM1000 is very simple to use with adequate precision functions for cell surgery the PM2000 will give users wider applications and more sophisticated programming functions PM1000 Cell Microinjector 100 120 or 240 VAC 35 Watts 60 100 psi Same as input pressure 0 3 psi regulated
15. a glass pi pette tip is the critical point of your application you need pulled quartz pipettes or quartz glass pipette puller The PMP 102Q is just developed and designed to pull quartz glass pipette With very successful technique and features designed for PMP 102 Programmable Micropipette Puller the PMP 102Q is continually adopted the advanced microcomputer control optical ruler measurement heat control and precision pneu matic pulling force control for the quartz glass pipette pulling Another bright point of the PMP 102Q is using regular cooking butane gas cartridge as heating gas source instead of using expensive and compli cate laser heating source The butane flame is optimal controlled by the PMP 102Q and well fit in a conve nient and compact platform With total savable 99 programmable sequences the flame pull force pulling distance timing can be set and programmed in up to 18 different steps in each sequence There are very useful standard sequences which are preset by MDI experts with numerous practices and experience A regular cooking butane cartridge can be used for long time and easy to be handled as operating a butane cooking stove in a PMP 102Q Without the focusing problem in a laser heater the PMP 102Q can pull pipette with 0 1 mm OD to 1 5 mm OD thin wall quartz glass tubing It can handle more pulling steps without a focusing problem The PMP 102Q is a practical compact precise control and reliable new quartz glass pipette
16. ces with 20 programmable steps in each se quence They can be used to perform and repeat different sequential pressure outputs precisely and automatically Also the sequential step cycles can be automatically performed or triggered each step manually All presettimers pressure reading memory and sequential steps are saved even turn off the power The PMFP is capable to perform repeatable experiments at anytime An attached foot switch can trigger preset sequence steps or be used as an interrupt trigger during output actions An optional remote keyboard give the user another convenient tool to control the whole opera tion The remote key board port can be interfaced with a computer control The exceptional versatility and precise multi output pressure control are the hallmark of the PMFP SPECIFICATIONS OUTPUTCHANNEL Eight adjustable pressure STEPSPERSEQUENCE 20 Programmable steps output channels One ad REMOTECONTROL Remote DB9 connector justable vacuum suction PORTS foot switch jack and Trigger channel BNC for negative TTL input LINE VOLTAGE 100 120 or 240 VAC OUTPUTSYNCHRO Eight BNCs for pressure POWERCONSUMPTION 40 Watts output port P1 P8 with INPUTAIRPRESSURE 30 93 psi positive TTL 0 5V signals PRESSUREOUTPUT 0 2 90 psi regulated output VACUUMOUTPUT 0 13 psi 0 25 Hg SIGNALPORTS One BNC for vacuum regulated suction output port P9 with REPEATABILITY 0 1 psi negative TTL signal 5V 0 DISPLAYRESOLUTION
17. ctrode Pulling In the PMP 107 series there are optional models of PMP107L r and PMP107L e The PMP107L r not only has the same function as the PMP 107 but also can pull extra length taper and tip up to 120 mm The application includes deep microperfusion and deep multichannel testing The PMP107L r features same rotation clamp on one side and flexible clamp head on the other side as the PMP 107 has While the PMP107L e is specially designed to pull extra long taper and tip for single to multipipette without twisting no rotation which can be inserted a long carbon or metal fiber inside the pipette for making a long electrode Since both pipette clamps on the PMP107L e are open ends an extra long fiber can be left out of both ends of pipette for pulling an extra long electrode Therefore the PMP107L e is straight pulling super length puller and excellent for long electrode fiber inside pipette pulling The PMP107 r can do rotating and straight pulling and is excellent for making long tip microperfusion and drug deep testing multipittes Specifications Multi barrel pipette Single to 7 barrel Each barrel pipette O D 1 mm 2 mm for single barrel Pullingforce Pneumatic Heater Nichrome coil or foil Heater control Microcontrollor Heating 74 general heat levels 24 99 64 automatic heat levels 45 98 Number of sequences 99 Steps of each sequence 18 Taper length setting Optical ruler 0 5 30 mm 0 5 120 mm for PMP 107L P
18. d fine moving adjustments in the model MFG 5P compare with an adaptable tool moving adjust ment in the MFG 5 The M2G 5P is a precision two grinding motors coarse fine microgrinder It can switche between two precision grinding motors coarse fine instead of between a forge heater and a grinder The model with microforge only is MF 5 The model with adding pressure air valve inside and foot switch outside is MFG 5A As a convenient and precision micropipette tip microforge microgrinder the MFG S5 can be combined with a PMP 102 or a PMP 107 micropipette multipipette puller to com pose of a complete and ideal micropipette production work sta tion To select the correct model please see the following tables Specifications Body Horizontal bino monocular microscope Eyepiece 10x 20xWE Optional 10x Scaled Objective lenses 4x 10x 0 25 Optional 20x 40x 0 6 Long Working Distance Light Two super bright white LEDs with ad justable back ground light Pipette Two axial manipulator and coarse fine movement focusing adjustment Heater grinder Two axial manipulator with three dimen movement sional adjustment Pipette holder TurnableEasy clamp for single or multipipette holding Heater Platinum wire heater combine of Microgrinder 0 1 1 0 um grinding disks with precise micromotor direct driving Air in output Optional air jet foot switch in output tub ing and connector Accessory One foot switch Optional one more Sy
19. ing the manipulator horizontally will select the heater or grinder in seconds also switching their power sup ply automatically There is a foot switch and heat speed level adjusting dial knob which control the heater or grinder operating There are two special super bright LEDs One is an adjustable powerful back light another is adjustable side light for a clear contour image The MFG Microforge Grinding Center is designed with com pact structure and precision control circuitry Microforging in the MFG 5 Microgrinding in the MFG 5 T MicroData Instrument Inc Precision Two Grade Microgrinder Coarse and fine erinding motors http www microdatamdi com The Microforge Grinding Center use high quality optical compo nents which include 10x or 20x wide field eyepieces and long working distance plan achromatic 4x 10x 0 25 40x 0 6 objec tives The 10x wide field scale eyepiece gives user another accu rate measurement tool Besides heater grinder control another optional foot switch connector can be added for pressure air con trol Outside pressure air can be input to the Microforge Grinding Center and can be switched output to a tubing by stepping on the foot switch The pressure air output can be used for clearing a pipette dust after grinding or expanding or cooling a pipette dur ing pipette forging A syringe and special connector tubing will come with the microgrinder for wed grinding There are precision coarse an
20. ion sequences with 32 pro counters and step sequences grammable steps in each sequence Each sequence can be repeated continually or triggered manually The interval time between steps can be programmed for automatic sequential cycle Manual control or automatic or manual trigger sequential step cycle operation There are two foot switch connectors for sequential step trigger and injection trigger A digital remote port can be connected to an optional remote mouse key pad or be controlled by a com puter With exceptional versatility and extremely precise control Programming information and pressure real time display both PM6000 and PM8000 are ideal multichannel injection perfusion engines MicroData Instrument Inc Tel 908 222 1717 Fax 905 222 1365 3 pinbr uj uloiq Connection for Intracellular Injection System M8000 or PM2000B Programmable Pressu e MDI MieroData Instrument Inc PM8000 Programmable Multichannel Pressure Injector o 20 90 psi Pressure Source re Injector o POWER DN FSW Foot Switch Connection for Extracellular Microperfusion System 50 90 psi Pressure Source PM8000 or PM2000B Programmable Pressure Injector tichannel Pressure bech MDI MicroData Instrument Inc PM8000 mmable Mul ec MD Mi
21. is available in the PM2000 The PM2000 allows the user to program custom designed action sequences Within each sequence auser can save4 pressure readings 4 preset timers and 4 counters for corresponding actions There are 12 programmable action steps in each sequence and total 63 programmable sequences MicroData Instrument Inc Tel 908 222 1717 Fax 908 222 1365 1 All 63 sequences will be saved even turn off the power The last using sequence will be recovered automatically when turn on the power again Therefore a user can program all different pres sures timers counters and action steps for different experiments up to 63 The user can just turn on the power to continue the last experiment or simply select a sequence number any one of 63 different experiments can be precisely repeated on any time A simple and straightforward keyboard allows the user to program and perform actions for the PM2000 or PM 1000 An LCD display window provides full information such as pressure vacuum PM2000 time count sequence steps PM2000 and actions etc Besides key pad there is a foot switch connector Specifications PM2000 Cell Microinjector 100 120 or 240 VAC 35 Watts 60 100 psi Same as input pressure 0 3 psi regulated 0 1 60 psi regulated 0 10 psi regulated 0 24 Hg regulated 0 25 of H20 regulated LINEVOLTAGE POWER CONSUMPTION INPUT GAS PRESSURE CLEARINGPRESSURE CLEAR HOLD
22. jection and cell electrophysiological appli cations Combining an advanced microcontroller and precise pneumatic components these systems can simultaneously con trol uptoeightinjection micropipettes oreight perfusion vials and one cell holding pipette and one drain pipette The four PM6000 or eight PM8000 injection perfusion output ports can be controlled separately or combined together to 24 perform actions such as injection capillary action balancing PM6000 4 Channel Pressure Injection System suction or clear up The cell holding output port can produce an adjusted gentle suction to hold a cell or eject a pressure to push a cell or completely clear the holding pipette Another drain KEY FEATURES output port can simultaneously produce an adjusted suction to Multiple function system for drain and clear out liquid before next channel microperfusion e NT d fas Both PM6000 and PM8000 can deliver different quantity agents microinjecuon ana pertrusion and drugs from picolitters to continued perfusion e ene o All output pressures and vacuums are regulatable and can be real ight in rf Up to e S t ject pe MHIL outputs time displayed on the front panel display Previous pressure one hold cell o utp ut and one readings can also be recovered on the display forsetting compari synchronized drain out channel son Besides programmable timers and counters there are 16 program Programmable and savable timers mable and savable injection perfus
23. ll surgery RNA DNA transfer chromosome dissection e Cytoplasm or nucleus extraction Cell selection pick up and movement Features Precision and versatile pneumatic injection engine for cell surgery and cell physiological research e Accurate microinjection suction clearing balancing and venting can be delivered from the cell injection port e Gentle sucking cleaning holding releasing and pushing a cell can be delivered from another cell holding port e Highest repeatability with action sequences for PM2000 pressure readings savable timers and counters All programming and actions are controlled by an advanced microprocessor User friendly keyboard foot switch remote mouse full in formation LCD display and remote control port for PM2000 Utilizing an advanced microcontroller and preci sion pneumatic components the PM2000 and PM1000 are just designed as two very precise and smart pneumatic engine for microinjection The PM2000 and PM1000 can deliver from picolitter to unlimited wide range of liquid to a target and can be controlled precisely to pick up and transfer sperm With a smooth and very fine adjustable suction ejection and a computerize timing valve every single sperm can be handled conveniently and precisely A 60 100 psi pressure input gas is the only gas source required for the PM2000 or PM1000 to produce a wide range of pressure and vacuum outputs There are two output ports on
24. me and length count heat level heat control and action parameters can be individually set in each step After a special sequence setting up a double identical micropipettes can be automatically pro duced by just pressing the START button on the PMP102 Pneumatic Pulling Force and Very Compact Size Comparing with other pipette puller using gravity or mag netic field as pulling force the PMP102 apply precise controlled pneumatic pressure as the pulling force which gives more controllable even and consistent dragging char acteristics With a double horizontal pulling the PMP102 can pull two identical ideal injection tips or microelec trode tips at the same time Within a very compact size the PMP102 can precisely and automatically perform multistep pulling without manual interrupt A precision micro linear ball bearing rail and advanced pneumatic com ponents are used to provide no fault pulling movement A simple 4x4 keypad and full information display LCD let users control easily and read all pulling parameters di rectly which include sequences steps time timing heat Tel 908 222 1717 Fax 908 222 1365 10 Pulling Samples from Preset Se quences level heater control tip length and actions The heater power control and acting time count up or count down are real time displayed With versatility EST SE of the intelligent PMP102 pulling an ideal micropipette is no longer an un Me cine certainty of hand skill but a repr
25. measure MicroData Instrument Inc Automatic Single to 7 Barrel Pipette Puller e Exclusive Optical Digital Ruler Measurement Computerize Real Time Feedback Heater Control Programmable and Savable Sequences for Creation and Reproduction Manufacture Preset Programs for Single 3 4 and 7 Barrel Micropipette Pulling Pneumatic Pulling Force and Compact Size Super Length Multipipette and Electrode Pulling Model the actual heating power during heating power on The mea surement will be real time displayed and feedback to the control unit to match the set point dynamically As a result the PMP 107 always provides precise heating power despite of many times of pipette pulling or thermal electrical characteristic chang ing Under a microcomputer controlling the heater is smart and reliable Programmable and Savable Sequences for Creation and Re production There are 99 user programmable and savable pulling sequences with maximum 18 steps in each sequence Users can easily pro gram different taper length tip shape for single to 7 barrel pi pettes Time number heat level heat control and action param eters can be individually set in each step After a special se quence setting up a multibarrel micropipette will be automati cally produced by just pressing the Start button Manufacture Preset Programs for Single 3 4 and 7 Barrel Micropipette Pulling Every PMP 107 will be well tested and installed sam
26. oducible automatic processing SEQ2 H P SEQ12 RH P R Manufacture Preset Programs for Major Different pipette pulls The PMP102 pipette puller is well tested and pre installed with a number of SEQ13 RH P different pipette pulling programs A user can easily find out a right sample 71 picture or the closest characteristic from the sequence list on the User s Manual Select the corresponding sequence and just pressing the START pe visi d key a desired pipette will be pulled automatically The preset programs are convenient and important not only because they can pull many different pipettes but also they can be used as templets to produce new programs With the copy function and just a few modifications from an exist sequence a new customize sequence program can be configured to fit a special re SEQ6 PH P SEQ16 RH P quirement As many as 22 sequences are developed and preset to the PMP102 c by spending a lot of test time and expertise These preset sequences are so important that users will save their time and pipettes without numerous pull SEGS PH P Se Optional Super Long Taper Pulling and Microforge Grinding Center Combination B E The regular PMP102 can pull up to about 20 mm setting length taper If a mm longer tap or tip is needed or pulling with a metal or carbon fiber inside a SEQ9 PHP SEQ19 RH P pipette is needed an optional model PMP107L e PMP102L can be se lected The PMP107L e will give yo
27. onal Model MFG 5 Microforge Grinding Center for single pipette to multipipette tip modification PMP 102L PMP 107Le Extra length pull up to 120 mm for the taper and tip It can straightly pull single or multibarrel pipette from 1 mm O D to 5 mm O D PMP 102m The PMP 102 model added with heater power and light power output connector air output connector two foot switch connectors for optional microforge M 1 MicroData Instrument Inc http www microdatamdi com e mail info microdatamdi com PMP 102Q Programmable Quartz Glass m Micropipette Puller SN Pull fused quartz fused silica glass micropipette with regu lar cooking butane gas High efficient micro torch with precision flame control without focusing problem Manufacture preset sequences within up to 99 Programmable Sequences Precise tip length setting with horizontal symmetric double pull g EF Z we iy be d 4 e 1 7 KL I dE e K E g je _ A EE 11 Hee BS bi b 03 E LI Quartz glass fused quartz fused silica pipette has much lower dissipation factor and dielectric constant than that of other glass Quartz pipette tip is much stronger than other glass too In another word the quartz pipette tip can be less noise in the patch clamp recording and not easy to break the tip when pen etrating tough tissue If these better electrical characteristics and better physical features of
28. options Prices and Specification All prices are FOB factory Prices and specifica tion are subjects to change without notice Ship ping and insurance are additional Minimum Billing Orders totalling less than 300 00 are charged an additional 15 00 for handling Warranty All products are warranted to be free of defects in materials and workmanship for one year from date of shipment Warranty becomes null and void because of abuse disassembly or unauthorized re pair of product Service Technical supports are available by directly call ing the office Repairs and modifications are also available for all our products Return Standard catalog items may be returned within 15 days from receipt but customer must pay all ship ments Returns after 15 days and before 30 days or will be subject to a 15 restocking charge No return after 30 days All return items and package should be maintained original condition and with out damage Used or contaminated pipettes tub ing and vials are not returnable Modified or spe cial order items are not returnable Contact MicroData Instrument Inc for return authoriza tion Unauthorized return will be refused Programmable Cell Microinjectors EE y C LP MM Eb EE PL ae ee zz PM 1000 Cell Microinjector Applications e Microinjection into adherent cells or into free floating cells with cell holding output e Intracytoplasmic Sperm Injection ICSI e Ce
29. p es 17 PN no 4 PMP 107Lr Programmable Super Length Multipipette Puller Automatic Single to 7 Barrel Pipette Puller With just pressing one key a multi barrel pipette can be successfully pulled by the PMP 107 Programmable Multipipette puller Equipped with a microcomputer pneumatic pulling arm precise automatic rota tor optical digital ruler and a new designed clamp the PMP 107 can automatically heat twist and pull one to seven barrel pipette There is no need for any manual rotation or any inconsistent timing interrupt control The whole pulling processing is programmable and under control of a preset sequence The PMP 107 is a new upgrade model from the PMP 100 multipipette puller Many new features have been added to the PMP 107 The rotation twist angle is adjustable Se quences are easy to copy and modify Rotation and pulling can act simultaneously while pulling distance is adjustable which can twist a multipipette with adjustable spirality Exclusive Optical Digital Ruler Measurement There is an exclusive optical digital ruler in the PMP 107 to perform precise taper length setting real time measurement and tip sensing With this feature a user can easily handle taper and tip pulling Computerize Real Time Feedback Heater Control In the PMP 107 there is an advanced microcontroller to perform real time heater monitoring and controlling If a heating level is selected and preset the microcontroller will
30. ple pulling programs for single 3 4 and 7 barrel multipipettes A new user will easily select the right program or just change a few step parameters to fit their special need The preset programs are convenient and important not only because they can pull dif ferent multibarrel pipettes but also as convenient copy tem plets to make other programs with only minor parameter change for other applications The PMP 107 has an easy copy program function Pneumatic Pulling Force and Compact Size Comparing with other pipette puller using gravity or magnetic field as pulling force the PMP 107 applies precise controlled pneumatic pressure as the pulling force which gives more con trollable even and consistent dragging characteristics Within a very compact size the PMP 107 can precisely and automati cally perform twisting and multiparameter multi pulling without inconsistent manual interrupt A precision micro linear ball bear ing rail and advanced pneumatic components are used to Tel 908 222 I717 Fax 908 222 1365 7 provide no fault pulling movement A simple 4x4 keypad and full information display LCD let users control easily and read all pull ing parameters directly which include sequences steps time timing heat level heater control tip length and actions With an intelligent PMP 107 pulling a multipipette is no longer an uncer tainty of hand skill but a reproducible automatic processing Super Length Multipipette and Ele
31. ption IP 1 Standard 6feet 1 4 O D inputtubing with con nectorto PM and LE swift fitconnector for source air Longer tubing can be selected OP 2 Teflon output tubing with connector to PM and male luerconnector for pipette holders or vials There are three sizes 1 8 2 0 2 2mm OD Teflontubingcan beselected OP 4 5 OP 10 OP 30 45m 10ml or30ml vial with In output tubing from topof vial and connectors to fit PM series OV 1 5 OV 5 OV 10 OV 30 1 5ml 5ml IOmlor30ml vial withinput female luer on top and output male luerin bottom STB 1 5 STB 4 5 STB 10 STB 30 OV orOP 1 5 4 5 5 I0or30 ml vial holder 5x or 8x vial holders can be selected STT 4 5 STT 30 OP 4 5 orOP 30 standing tray whichcanhold9 of OP 4 5 or20 of OP 30 f 2o Aa OP 4 5 OP 10 OV 30 PP 7 enlarged PP 4 enlarged Rolair JC10 Compressor PP 4 Pulled 4 barrel pipette PP 7 Pulled 7 barrel pipette HOLD 1 Pipette holder for single pipette 1 1 2mmO D HOLD 4 Pipette holder for 4 barrel pulled pipette 1 mm O D each barrel with 4x 1 32 LD Tygon tubing amp female luerconnector HOLD 7 Pipette holder for 7 barrel pulled pipette 1 mm O D each barrel with 4x 1 32 LD Tygon tubing amp female luerconnector FSW FootSwitch for PM senes and Microforges MOUSE 1 Very convenientremote control of Injection Hold and HillforPM1000 MOUSE 2 Very convenientremotecontrol of Injection Auto and Hill for PM2000 PM
32. ressurel Adjustable 0 1 10 psi Pressure2 Adjustable 0 1 60 psi Cooling Pressure Adjustable 1 30 psi Rotation Control Turning angle 90 135 180 and 225 degree adjustable Gas input pressure 30 60 psi Actions Pull 1 Pull 2 Pull 2 Cool Rotation Rotation pull Cool Air and Return Display 20x4 LCD Powerinput 110 240 VAC Power consumption Maximum 150 watts Dimension 14Lx 11W x 7H inch Weight About 18 Ibs MicroData Instrument Inc http www microdatamdi com Unpulled 7 4 barrel Pipettes Pulled 7 4 barrel Pipettes Pulled 7 barrel Pipette Tip 100 um _ _ Pulled 7 barrel Pipette With 100 mm Long Tip Ee Basic Accessories Spare heat coil Tweezers 12 preset sequences and samples Input tubing and connector Power cord User s manual Optional Accessories MFG 5 Microforge Grinding Center for single pi pette to multipipette tip modification Air Compressor Pump for PMP 107 air source Optional Model PMP107L r Extra length pull up to 120 mm for the taper and tip Selected fix pipette clamp for both sides or one side clamp rotatable another side flexible PMP107L e Extra length pull up to 120 mm for the taper and tip Fix and open end pipette clamp for both sides e mail info microdatamdi com PMP 102 Programmable Micropipette Puller ek PMP107L Multipipette Puller C52 PUREE PAESIURE mun ee r gee mm 989 Te a PM
33. ssure valve air jet foot switch and mi output connectors Two foot switches Syringe with special connector tubing In output air tubing 30W 120 240VAC Ls MFG 5AP Horizontal Binocular microscope 10x 20xWE Optional 10x Scaled 4x 10x 0 25 Optional 20x 40x 0 6 Long Working Distance Two super bright white LEDs with adjust able back ground light Two axial manipulator and coarse fine fo cusing adjustment Precision manipulators with three dimen sional coarse fine setting and adjustment Turnable Easy clamp for single or multipipette holding Precision platinum wire heater combine with 0 1 0 5 1 0 um grinding disks with precise micromotor direct driving Pressure valve air jet foot switch and in output connectors Two foot switches Syringe with special connector tubing In output air tubing 30W 120 240VAC MFG 5APT w Digital Camera e mail info microdatamdi com Micropipette Work Station PMP102 and MFG 5 combination PMP102 Programmable Micropipette Puller MFG 5A Microforge Grinding Center MFG 5AP Precision with Pressure Port Microforge Grinding Center A with Pressure Port Model Selection MF 5S MF 5 MF 5A MF SAP MG 5 M26 3AP Micro Heater Microgrinder V 1 V 2 Heater Grinder Combination Pressure In output Binocular B Monocular S Two axial manipulators Coarse Fine manipulator T MicroData Instrument Inc Tel 908 222 1717 Fax 908 222
34. to the control unit to match the set point dynamically As a result the puller always provides precise heating power despite of many times of pipette pulling or thermal electrical characteristic changing MicroData Instrument Inc e Press only one key to produce two identical single pipettes with different preset and pro erammable sequences e Pull different patch clamping intracellular elec trodes injection micropipettes and microneedles with 99 programmable sequences Microprocessor real time controlled system performs programmable multipulling steps op tical digital measurement and precise digital feedback heating control to achieve exceptional flexibility and repeatability e Consistent pulling force by utilizing pneumatic pressure instead of gravity or magnetic field e22 manufacture preset sequences and samples for different application pipettes Optional microforge heater power supply air jet output and foot switch connectors If the heating level is set to AUTO the heater will automatically search the melting point for different glass pipettes Under a microcomputer controlling the heater is smart and reliable Programmable and Savable Sequences for Creation and Reproduction There are 99 manufacture user programmable and savable pull ing sequences with 18 steps in each sequence Users can easily program different pipette tip size tip length and tip shape in different sequence for different application Ti
35. ts trigger and interrupt signal input and trigger interrupt foot switch Convenient full function control key pad and remote port for remote key pad and computer interface The PMFP Programmable Multichannel Microfluidic Pump is designed to drive solution for variers microfluidic devices microfluidic logics flow cells and microfluidic chips applications Upto eight pressure outputs and one suction vacuum output can be controlled separately or with different channels combination together to perform actions such as output different pressures and suction different timing and different automatic sequences MicroData Instrument Inc es P Tel 9O8 222 1717 Fax 9O8 222 1365 es There are up to nine individual regulators for adjust ments of eight channel output pressure and one suction vacuum An LCD provides full information display such as pressure vacuum time sequence steps and actions During pressure mode all pres sure readings are savable for next time repeat operation The previous pressure reading can be recalled on the LCD for new pressure setup com parison The PMFP provides programmable timers for every independent output control The PMFP also provide different sequence step time to deliver accurate timing and duration for each step channels action The PMFP allows the user to operate manually or automatically multistep sequences running which are designed by user There are 15 savable se quen
36. u up to 120 mm long taper or tip pulling Some special modifications such as angle grinding polishing and bending SEDE Io DE for a pulled tip are required by using a microforge and grinder There is perfect combination of a PMP102 Programmable Micropipette Puller with SEQ22 RH P SEQDTRHP a MFG 3A Microforge Grinding Center to form a micropipette production 8 mmm Work station The two units combination will give you total capabilities and seamless ideal continuity for capillary glass micropipette making Specifications Pipette Single barrel Imm 2 5mm O D Sequences Total 99 Display 20x4 LCD borosilicate or aluminosilicate glass Steps 18 per sequence Heater power output Optional capillary tubing Taper length setting 0 5 20mm Light power output Optional Pulling force Pneumatic Pressure 1 regulator 0 1 10psi adjustable Pressure air output Optional Heater Nichrome coil Pressure 2 regulator 0 1 60psi adjustable Foot switch connector Optionaltwo Heat Stop Air Out Heater control Microcontrollor Cool air pressure 0 5 30psi adjustable Power input 110 240 VAC Heating Level 76 generalheat levels 24 99 54au Pressure gas input 30 60psi Power consumption Maximum 150 watts tomatic heat levels 45 98 Actions Pull 1 Pull 2 Pull 2 Cool Dimension 18 Lx8 Wx 11H Cool Air and Return Weight 23lbs Accessories Spare heat coil Tweezers 22 preset sequences and samples Input tubing and connector Power cord User s manual Opti
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