NIBP250 User`s Guide
Contents
1. button 3 Verify that Tail Cuff Tubing Port see 2 2 Rear Panel is open to atmosphere 4 Check the Current Pressure value on the screen and verify that it displays 0 0 mmHg If not then recalibration is necessary 5 Connect a manometer and an inflation bulb to Tail Cuff tubing port on the rear panel see 2 2 Rear Panel Verify that there is no leakage in the setup Using the inflation bulb increase the system pressure to 100 mmHg Be careful not to over inflate the cuff 8 Compare the pressure value shown by the manometer and the Current Pressure value on the NIBP250 s screen 9 Ifthe two values match the calibration is validated If not recalibration is necessary 3 9 2 Recalibration 1 With the NIBP250 turned off disconnect the Tail Cuff Sensor 2 Turn on NIBP250 and display the Calibration Tab 3 Verify that the Tail Cuff Tubing Port see 2 2 Rear Panel is open to atmosphere Press Cal 1 button to record the sensor signal at 0 mmHg Oi Connect a manometer and an inflation bulb to Tail Cuff tubing port on the rear panel see 2 2 Rear Panel Verify that there is no leakage in the setup Using the inflation bulb increase the system pressure to 100 mmHg Press Cal 2 button to record the sensor signal at 100 mmHg oo N 2S Press OK button to save the new calibration parameters 3 10 Triggers The NIBP250 has trigger input and output connections The trigger input has2. a TTL compatible circuit and a pull up resistor To start a measurement using the trigger Enable the Trigger Input option checkbox in the Options Tab see 2 5 2 Options and then press the Start button NIBP250 will wait until the trigger is initiated before acquiring the measurement To trigger the measurement the trigger input signal must be set low The trigger output also has a TTL compatible circuit The output starts at 0 volts and increases to 5 volts during the measurement process info biopac com Page 13 of 18 NIBP250 User s Guide 4 Using the NIBP250 with a BIOPAC MP Acquisition System 4 1 Connections 1 Connect the CBL150 PRE cable a BNC to the pressure output on the back panel of the NIBP250 b Other end to A1 on the front of the UIM100C unit 2 Connect the CBL150 PLS cable a BNC to the pulse output on the back panel of the NIBP250 b Other end to A2 on the front of the UIM100C unit 3 Connect the IRSENSOR a Black cord to the sensor input on the back panel of the NIBP250 b Tubing in the cuff on the front panel of the NIBP250 4 Connect the power a AC300 adapter to the 12V DC input on the back panel of the NIBP250 b AC300 to Mains power 5 Switch the NIBP250 POWER on CBL150 PRE and CBL150 PLS cables are used with BIOPAC MP150 Data Acquisition Unit with Acq Knowledge software only For use with BIOPAC MP36 35 Data Acquisition Unit with BSL PRO software use the CBL35 PRE for Step 1 and
3. tacenseestecacneaedascep esate 15 D MITOSIS E EE EEA AE E EEEE 16 6 1 Tail Pulse signals are not regular cccccccseseccceseeceeseeceaeecseseeceaceeeaececeuecenagecesageesseeessageeseeeensueeeneeesens 16 6 2 Compressor FUNS CON NUOUS Y sssisiisiuscsrsninasninninniannin annannati aaa 16 6 3 There is leakage from the tubing and Tail Cuff Sensor connections cecccceeececeeeceeeeeesaeeeseeeeseneeeeas 16 Ti PCS SS E E E E EA E E EA A E N EN A A A O A A 17 8 Maintenance and Or orsin eee ne ne en eee ee 18 oM ree IS FON car sets I EA E erent wot Bessie E O A E AE E eluent crepe E AE 18 9 2 Cleaning Proca dU oS een ee en mee enn OE EEES 18 8 2 1 Monitor amp Sensor Housing Cleaning Procedures cccccseececeeeeeeeeeeeeeeeseeeeeeeeeeeeesueeeeesaaeseesaaeeeeeaaees 18 Index of Figures POO PRONG ANO nar E EE E E E E E E E EE 4 FOUG Roar FAC eee ra Er Er E E E E EEE EE E E EER 5 POUES O O a a E EEE E E A E E EE E 5 Figure 4 Main Screen ui iairecexetcencninasapncsinniamastmnienenseudemnasinn sep uawiabaintuuiddalemndiietanieonemetanhoelaniabuatsubawoudinadeusdiunbebysaediaivaniewimcecneeie 6 Figure 5 Analysis ViCW ccccccccssscccssscecesececeueccnaseeesaseeceaseensaececeuececegecesseesageeesaeeeesaeeensecenegecesaqeeesaneessassereneseesegess T Figure 6 Menu WINKOW cccccsescccseececesececeueccnsseecsaseccsaseessaeceseuececaeeesaeeesageeesaeeeeseeensuetensueseseseeesaneessaesereneesena
4. 1 Pressure cable to MP System or third party A D hardware 1 Pickering TG Hall JE Appel LJ et al 2005 Recommendations for blood pressure measurement in humans and experimental animals Part 1 blood pressure measurement in humans a statement for professionals from the Subcommittee of Professional and Public Education of the American Heart Association Council on High Blood Pressure Research Hypertension 45 5 142 61 info biopac com Page 3 of 18 NIBP250 User s Guide 1 4 Animal Preparation 1 Turn the Animal Heating Chamber on 2 Set the temperature value press and hold P Set and then press the up or down arrow to reach the desired value A For accurate noninvasive blood pressure measurement the animal or its tail should be warmed to 32 C Press the Heater button to start heating to the selected temperature value Place the animal inside the RESTRAINER Animal Holder select the suitable size for the animal s size weight A Leave the tail outside A Adjust the length to obtain a position where the animal has limited movement Place the RESTRAINER with the animal in the heating section of the Animal Heating Chamber Wait approximately 30 minutes for the animal to reach the selected temperature Remove the RESTRAINER from the Animal Heating Chamber Connect the Tail Cuff Sensor to the animal s tail O O N o p Verify that the sensor fits the tail correctly The sensor should be attached between the mid po
5. CBL35 PLS for Step 2 NOTE BIOPAC recommends connecting the CBL150 PRE cable to the first channel A1 so that the pressure wave is automatically displayed above the pulse waveform 4 2 Software Setup in AcqKnowledge 1 Laun ch AcqKnowledge 2 Select the Create Record a new experiment option 3 Select MP150 gt Set Up Channels gt Add New Module From the new module list select UIM100C A1 or whichever channel CBL150 PRE pressure cable is connected to and click Add b From the UIM100C Transducer list select NIBP250 Small Animal Tail BP Pressure and click OK c Click Calibrate in the resulting NIBP250 Calibration dialog a AcgKnowledge NIBP250 Calibration Make sure the animal tail is notin the IRSENSOR of the NIBP250 Press Calibrate emes 4 Repeat Add New Module portion of Step 3 From the new module list select UIM100C A2 or whichever channel CBL150 PLS pulse cable is connected to and click Add b From the UIM100C Transducer list select NIBP250 Small Animal Tail BP Pulse and click OK a Page 14 of 18 www biopac com Noninvasive Blood Pressure Monitoring System 5 Technical Specifications Table 4 Technical Specifications Specification Value Cut off Pressure Range 100 300 mmHg adjustable by 50mmHg steps Pressure Accuracy 300 mmHg Full Scale 1 Pressure Sensitivity 0 1 mmHg Pressu
6. NIBP250 User s Guide Noninvasive Blood Pressure Measurement System a TAIL IRCUFFSENSOR 9 5 Ga BIOPAC Noninvasive NIBP250 Systems Inc Measurement Sys AS ae Systems Inc 42 Aero Camino Goleta CA 93117 Telephone 805 685 0066 Facsimile 805 685 0067 support biopac com www biopac com NIBP250 User s Guide Table of Contents User s Manual 1 Introduction cccccccccccecccececeeeeceeeeseeeeeeeeeeeeeeeeeeeeeeseeeeseeeeseeeesaeeseeeseeeseueesseesseseseueeseeeeaeeeeaes 3 t1 SEMEL all DESCMPUON sssri a a e A E idesanedcwctensd 3 1 2 Theory of Measurement ccccccssseccccesseecceeseeeceaeeeceeuseeccauseeessaueeecsaeeecsaseessaueeessageeeesansueeeseeeesssaueeessageees 3 1 3 What s Included in System cccccscccccssseecceeseeeceseeecseececseasceeseaueeecsaecessseeeseaaeeessageeeesansgecesseeessseseeessageees 3 1 4 Animal Preparation sss 5 ete spe eserss Bae eete neces Cae dee avd celeneeiedicannn Sete hene tenes des eae tes eena dee nn aaenennencheaeceseesiceuaensed 4 2 User Interface ANd CONNECTIONS cccccccceecceceeeeceeeeeeeececeeececaeececeeeeeseeeseeeessaeeesseeeessueeeseueessesecessueeeseneeesanees 4 2 FTON PANO orane aise onde ne ce E EE E EO EE E E 4 22 ROI PANGI oe EEE E EEEE EE aw AE E EAE EE 5 PAE ES EE EEN E AT ATT A E E E A E T T AT E E 5 24s MaN SS CVC O seia a E seen RE EA a EEEE EA a EEE e E 6 2 4 1 Measurement Analysis View ss scsiewsinvi
7. The NIBP250 Tail Cuff uses an infrared sensor to detect the systolic and diastolic pressures These pressure values are computed differently than a standard sohygmomanometer cuff In a standard cuff using the auscultatory method the diastolic pressure is determined by the last pulse detected as the signal falls back to baseline In the NIBP250 which uses the oscillometric method the diastolic pressure is aligned with the peak pulse detected Figure 12 on the following page illustrates the location of the systolic diastolic pressure values on the NIBP250 Main Screen Page 10 of 18 www biopac com Noninvasive Blood Pressure Monitoring System Peak pulse detected Diastolic BP a E 0 462753 W Paak trpi A TIPIT TOE Ti Phen Ween p a j j HE f PAT nii i i Hil F Ty l i hit p CETA i a i Figure 12 Location of Systolic and Diastolic Pressures The NIBP250 s automated peak detection system marks the peak of each pulse with a white cross and is enabled by selecting the Peak by peak option on the Main Screen This feature makes it easier to identify the individual pulses To determine the systolic and diastolic values 1 Select the Peak by peak box on the main screen 2 Use the right or left cursor button to locate the first pulse s white cross and press the Systolic button You may also place the cursor using the touch screen The system will record and display the systolic blood pressure value 3 U
8. arked for analysis The NIBP250 returns marked values for systolic and diastolic blood pressures and calculated values for mean blood pressure and heart rate The NIBP250 does not require a data acquisition system or computer to analyze the data but can interface with existing BIOPAC data acquisition platforms and third party systems when combining other physiological signals 1 2 Theory of Measurement The NIBP250 noninvasive blood pressure system uses an adapted version of the well known Oscillometric Technique Oscillometric Technique was first demonstrated in 1876 and involves the observation of oscillations in the sohygmomanometer cuff pressure which are in turn caused by the oscillations of blood flow i e the pulse An electronic version of this method is sometimes used in long term measurements and general practice This method relies on a sohygmomanometer cuff with an electronic pressure sensor transducer to observe cuff pressure oscillations By use of this type of sensor the NIBP250 automatically acquires BP data and controls the automatic inflation and deflation of the cuff The cuff is initially inflated to a pressure in excess of the systolic arterial pressure and then reduced over a period of about 30 seconds to just below diastolic pressure When blood flow is nil cuff pressure exceeding systolic pressure or unimpeded cuff pressure below diastolic pressure cuff pressure will be essentially constant When blood flow is p
9. ed Measurements that do not return any results are identified by a value of 1 0 info biopac com Page 9 of 18 NIBP250 User s Guide To load a measurement touch the desired row and press the OK button Result Number Sys Da ean HR Description Date O ROOOOOO nbp 22 100 14 713 Description 1 Li22fe0l2 4 25 56 PM ROOOOOOS nbp 0 000 0 000 Description 1 Lz22f2012 4 39 34 PM 30 325 17 094 Description 1 1 22 2012 5 19 26 PM 155 800 109 300 Description 1 Lj24f2012 4 51 06 PM 145 700 101 600 Description 1 1 25 2012 4 40 58 PM ROOOOO21 nbp 179 900 124 100 Description 1 125 2012 4 42 08 PM Cancel Figure 11 Results List 3 Usage 3 1 Start up and preparation With unit turned off attach the sensor and cuff connectors Turn on unit and wait for the Main Screen to appear Prepare the animal and attach sensor cuff to tail 3 2 Acquisition When preparation is complete Press the Start button on the Main Screen The button label changes to Stop and you can halt the acquisition at any time When the acquisition starts the unit automatically closes the leakage valve and begins inflating the cuff After pressure reaches the maximum level the pump stops and opens the leakage valve to release the pressure After the pressure is fully released the acquisition stops 3 3 Analysis Following the acquisition the unit automatically detects the pulse peaks after which a cursor will appear on the main screen NOTE
10. ed the NIBP250 automatically detects the peaks of the pulse signal and marks them with white crosses as seen in Figure 5 Touching anywhere on the plotting will bring up a cursor Wherever the cursor is positioned the pressure and pulse amplitude will be displayed If the Peak by peak option is selected the value of the peak is displayed If unchecked the value of the measurement sample is displayed To record systolic and diastolic points move the cursor to the desired axis position and press the Systolic or Diastolic buttons The pressure values at those points will be listed in the Results section along with the Mean pressure and Heart Rate values On the analysis window the current measurement may be saved by pressing the Save button If it s saved after marking the systolic and diastolic points the saved data will also include these values in the analysis results info biopac com Page 7 of 18 NIBP250 User s Guide 2 5 Menu Window The NIBP250 Menu window see Figure 6 can be displayed by pressing the Menu button on the Main Screen while no measurement process is running The General Options and Calibration tabs are used for accessing and adjusting the NIBP250 global settings Pressing OK in the Menu window saves the settings to the device memory Pressing Cancel discards any changes General Options Calibration System date amp time 16 36 19 25 01 2012 Set Pulse
11. een NIBP250 User s Guide The touch sensitive Main Screen see Figure 4 controls all operational and setup functions All buttons on the right of the screen are also touch sensitive For a full explanation of onscreen buttons see Table 2 on page 6 10 Time s5 16 33 50 25 01 2012 Cursor Peak by peak Set as Systolic lean Heart Rate m se Figure 4 Main Screen Table 2 describes the user interface buttons and their functions Button aa m Peak by peak Systolic Diastolic Load Page 6 of 18 Table 2 Main Screen buttons Functionalit Moves the cursor to the left lt lt or right gt gt by one step If Peak by peak is enabled the cursor moves to the previous or next peak Sets the current position of the cursor as the systolic pressure point or the diastolic pressure point Displays the saved measurements in the Results screen Button Functionality Saves the last measurement Save Starts the BP measurement process Start If the Trigger Input active option is selected it waits for activated trigger input See 2 5 2 Options Displays the Menu Window Menu See 2 5 Menu Window www biopac com Noninvasive Blood Pressure Monitoring System 2 4 1 Measurement Analysis View Peak by peak Set as Systolic Results ROOOO022 Mean 111 6 Time s Figure 5 Analysis View If the Peak by peak option is select
12. erted or removed while the NIBP250 is actively loading or saving a measurement 3 8 Firmware Update The NIBP250 device firmware should be periodically updated For firmware updates check the NIBP product web page at www biopac com and download the NIBP250Update cab file Then copy the file to a FAT formatted SD Card or USB flash drive To update the firmware 1 With the NIBP250 turned off insert the SD card or flash drive containing the firmware update file into the slot or USB port on the NIBP250 rear panel see 2 2 Rear Panel 2 Turn on the NIBP250 The unit automatically detects and updates the firmware after which it will initiate its regular startup cycle Page 12 of 18 www biopac com Noninvasive Blood Pressure Monitoring System 3 9 Calibration The NIBP250 is factory calibrated User calibration of the pressure sensor prior to first use is not required However if desired the sensor can be checked for calibration accuracy and recalibrated if necessary The Calibration Tab is displayed by pressing the main screen Menu button see 2 5 3 Calibration When the Calibration Tab is displayed NIBP250 automatically closes the leakage valve to keep the pressure constant during the calibration check or recalibration process 3 9 1 Calibration Check To check the calibration 1 With NIBP250 is turned off disconnect Tail Cuff Sensor 2 Turn on NIBP250 and display the Calibration Tab using the main screen Menu
13. gain 1 Gee Cut Off Pressure 200 10 Time s Figure 6 Menu window 2 5 1 General Tab The General Tab see Figure 7 is where the NIBP200 date and time is set or adjusted Press the Set button to display the Date Time Properties see Figure 8 General Options Calibration System date amp time 16 36 19 25 01 2012 Set Pulse gain 1 Time zone Cut Off Pressure 200 h GMT 02 00 Athens Bucharest I O OK O Cancel Automatically adjust Ook canei u gT clock for daylight saving ikea 3 41 09 PM Figure 7 General Tab Settings Figure 8 Date Time Properties window Set Date Select the desired calendar day and use the right left arrow buttons to set the month year Set Time Select the desired time unit AM or PM seconds minutes hours and adjust via the arrow buttons Set Time Zone Press the downward arrow to the right of the Time Zone field and select the desired zone from the list Page 8 of 18 www biopac com Noninvasive Blood Pressure Monitoring System After setting the Date Time Properties click OK at the top right of the window to accept the new settings To discard settings without saving changes click the X button The Pulse gain and the Cut Off Pressure are adjusted by moving the sliders left or right Click OK to accept any modified settings Click Cancel to discard 2 5 2 Options Use the Options Tab see Figure 9 to adjust t
14. he triggering parameters If an external trigger to be used to initiate the start of a measurement enable the Trigger Input active option General Options calibration i Trigger Input active Auto save after trigger cancel Figure 9 Options Tab Settings If Auto Save after trigger is enabled the device will automatically save the last measurement 2 5 3 Calibration In the Calibration Tab see Figure 10 the pressure sensor calibration can be checked and recalibrated if necessary General Options Calibration Point 1 for 0 mmHg 0 0000 Cal 1 Point 2 for 100 mmHg 1 0000 Cal 2 Current pressure mmHg 3 4 Current signal Wi 0 0342 Calibration line YtrimHg 100 0 xx 0 0 Restore default calibration cancel Figure 10 Calibration Tab Settings The Calibration Tab displays the raw sensor values for two calibration points at 0 mmHg and 100 mmHg current pressure and raw signal values and the calibration line formula The Cal 1 and Cal 2 buttons are used for measuring and setting the raw signal values for each calibration point Pressing the Restore default calibration button reverts the calibration setting to it s factory default 2 6 Results List The Results List window see Figure 11 lists the previously saved measurements For each measurement the Result Number Systolic Diastolic Mean pressures heart rate description and save date time are display
15. inger on the Cuff output and press the Start button The compressor will work for a few seconds and stop please inform BIOPAC if the Compressor does not stop The pressure chart should be viewable on the screen If the Compressor stops automatically it means that the system is working normally 6 3 There is leakage from the tubing and Tail Cuff Sensor connections A Make sure the tubing connections are securely attached Page 16 of 18 www biopac com 7 Accessories Noninvasive Blood Pressure Monitoring System Table 5 lists the available accessories for the NIBP250 system info biopac com Table 5 Accessories item Description RXTCUFSENSORSY 5 Tail Cuff Sensor 9 5 mm RXTCUFSENSOR11 Tail Cuff Sensor 11 mm RXTCUFSENSOR13 Tail Cuff Sensor 13 mm RXCUFSENSY 5 MRI Tail Cuff Sensor for MRI 9 5 mm RXCUFSEN11 MRI Tail Cuff Sensor for MRI 11 mm RXCUFSEN13 MRI Tail Cuff Sensor for MRI 13 mm RXRESTRAINER S Restrainer for Rat Sm replacement RXRESTRAINER M Restrainer for Rat Med replacement RXRESTRAINER L Restrainer for Rat Lg TAILHEATING B Tail Heating Unit 220V 50Hz TAILHEATING A Tail Heating Unit 110V 60Hz Page 17 of 18 NIBP250 User s Guide 8 Maintenance and Care 8 1 Service Policy The display unit of the NIBP250 does not require any routine maintenance Should service be required it should be performed by a
16. int of tail and base of tail spinal column 10 Wait for the animal to relax and become inactive before starting measurements Prior to conducting the experiment condition the animal by putting it inside the holder several times a day and repeat the tail heating each time It is also advisable to turn on the sensor a few times so the animal becomes familiar with the feel of the occlusion process 2 User Interface and Connections 2 1 Front Panel ZA BIOPAC NIBP 250 EEn u Figure 1 Front Panel Figure 1 shows the front panel of NIBP250 It has a 7 LCD Display with a resistive type touch screen user interface To activate controls touch the screen with your finger or the stylus Page 4 of 18 www biopac com Noninvasive Blood Pressure Monitoring System 2 2 Rear Panel 00 D amp SENSOR CUFF lt ee Figure 2 Rear Panel Table 1 Rear panel connections 1 On Off Switch 6 Trigger Output 2 Power Adaptor Input 7 USB Memory Slot 3 Pulse Signal Analog Output 8 SD Card Slot 4 Pressure Signal Analog Output o Tail Sensor Input 5 Trigger Input 10 Tail Cuff Tubing Port 2 3 Sensor Figure 3 shows Tail Cuff Sensor and its details TAIL Cuff IRCUFFSENSOR 9 5 __ Cuff Tubing _ Figure 3 Sensor IMPORTANT The correct tail cuff size must be selected for the animal For cuff sizes see Table 5 on page 17 info biopac com Page 5 of 18 2 4 Main Scr
17. n the directory Measurement files are in ASCII format and can be imported by any text reader The measurement file content is a CSV file type with a semicolon separator and easily imported into common spread sheet applications Table 3 shows a sample measurement file with a description of each line in the file Table 3 Measurement File Format Line Data Measurement File Description Content NIBP Result File i type remark ile name 3 aaa aes Systolic point time pressure value 4 6 4 179 9 Diastolic point time pressure value 5 13 4 68 4 Mean pressure value Heart rate value 6 124 1 1 0 Description text T Decor on l Data sample period in seconds 8 P Number of data lines 9 1 0 004 Pressure value Pulse value at time 0s 10 6001 Pressure value Pulse value at time 0 004s 20 4647 0 0246235396 20200505 00279067909 6007 5999 Pressure value Pulse value at time 23 992s 6008 6000 Pressure value Pulse value at time 23 996s 6009 6001 _ Pressure value Pulse value at time 24 000s 93497 0405229580015 s32 L9lL 0 052760648594 8 33984 0 0527648594 3 7 External Memory Units The NIBP250 has a USB 1 1 compatible flash memory port and SD card slot External memory units must be formatted to a FAT file system prior to use External memory units are automatically detected when connected after which measurement data can be transferred to a computer for further analysis or publishing External memory units should not be ins
18. ness 8 Figure 7 General Tab Settings ccccccssccccsseeccseececseuceccueeeceueeceaseessaeeecsaeeeeseeessaueessagseeseaeeessaesecsueeessageessgsessags 8 Figure 8 Date Time Properties WINGOW cccccsecceeceeeeeeeneeeeeeeeeeeeeeeeeeesseeeeeeseeeeeesseeeeeeseeeeeesaeueesaeueeessaeeeessaeseetsaess 8 Figure 9 Options Tab Settings ccccccccccssscccceesececcesseecceeseeeceegeeecseaseeessaueeecsaeesseaseeessageeecseseeessageeessegeeessageesesaaes 9 Figure 10 Calibration Tal SCTUNGS sccsrncdensernnacsanciwnsseuthnadretaadenaetmbatnenibatee wana EA OAE AA Nirea R EERE Ei oaan 9 POAST RSU EIS ee a N EEE E A EEE EE E ee EEE AE 10 Figure 12 Location of Systolic and Diastolic Pressures ccceccccceeeceecaeeeeeeaeeeeesaeeeeesaeeeeeeseeeeeessaeeeessaeeeeesaeeeeeas 11 Page 2 of 18 www biopac com Noninvasive Blood Pressure Monitoring System User s Manual 1 Introduction 1 1 General Description The NIBP250 noninvasive blood pressure monitor is a stand alone device for measuring the blood pressure of small animals The NIBP250 has a convenient touch screen LCD user interface and includes a built in Tail Cuff Sensor pump that automatically inflates the cuff to occlude the blood vessels in the tail of a rat or similar small animal Once the pump reaches maximum inflation point the cuff slowly deflates providing a linear drop in pressure Using the simple touch screen interface the blood pressure points are easily m
19. qualified technician All maintenance and repair procedures should be directed to BIOPAC To obtain information on or order repair parts contact BIOPAC BIOPAC Systems Inc 42 Aero Camino Goleta CA 93117 Telephone 805 685 0066 Facsimile 805 685 0067 E mail support biopac com Web www biopac com For authorization and instructions for repair service contact your local Field Service Representative Provide the following information Your Name Unit Serial Number Your Institution Sensor Size Address Describe the problem Telephone Number Facsimile Number E mail Address You will be advised of the corrective action to take 8 2 Cleaning Procedures Warning Do not sterilize the sensor by autoclaving steaming irradiation or ethylene oxide ETO Do not soak sensor or any part of the NIBP250 system in any solution or water 8 2 1 Monitor amp Sensor Housing Cleaning Procedures The monitor and sensor may be cleaned on an as needed basis To clean the monitor and sensor dampen a cloth with a commercial nonabrasive cleaner and wipe the surfaces lightly Always apply the solution to the cloth not directly to the monitor and sensor Do not immerse any part of the NIBP250 system Rev 06 07 2013 Page 18 of 18 www biopac com
20. re Signal Output 300 mmHg 3 Volt DC Pulse Gain Levels x1 x2 x4 x5 x8 x16 x32 adjustable Pulse Signal Output 0 5 Volt DC LCD Display T 800x480 TFT User Interface Resistive Touch Panel Analog outputs Two BNC connectors for uncalibrated pressure and pulse signals Triggers Two BNC connectors for TTL Compatible trigger input and output signals Power Supply 12 Volt 2 Amp External info biopac com Page 15 of 18 NIBP250 User s Guide 6 Troubleshooting 6 1 Tail Pulse signals are not regular A The animal may be under stress resulting in excessive tail movement Remove the animal from the RESTRAINER holder until it calms down before continuing with the experiment The tail may not be sufficiently warmed or cooled down Put the animal in the Tail Heater Chamber and repeat the heating process Make sure the tail temperature is 32 C Tail Cuff sizing may be incorrect Check Table 5 on the following page for sizing descriptions Tail Cuff Sensor position may be incorrect Try re attaching the sensor in a different location on the tail The optimal location is between the mid point of tail and base of tail Spinal column 6 2 Compressor runs continuously A A A A A Immediately Turn off the NIBP250 system Remove the Tubing from the Cuff connector on the rear panel of NIBP250 Turn the system back on Close the air outlet by pressing the f
21. resent but restricted the cuff pressure monitored by the pressure sensor will vary periodically in synchrony with the cyclic arterial expansion and contraction i e it will oscillate The systolic and diastolic pressures are computed using an algorithm and the computed results displayed For human use blood pressure cuffs are snugly wrapped around the upper arm at roughly heart level The blood flow rate in the upper arm is measured to determine whether the flow is impeded systolic or unimpeded diastolic For use in small laboratory animals the cuff is placed around the tail However the blood flow rate in the tail is normally too low for the oscillations to be detected with a sohygmomanometer cuff The NIBP250 system accurately measures systolic and diastolic pressure levels using a Tail Cuff Infrared Pulse Sensor to detect blood flow pulsations To enhance measurement accuracy it s advisable to gradually heat the tail of the animal using the optional Tail Heating Unit 1 3 What s Included in System Each NIBP250 system includes e 1 amplifier unit switching power supply works for 110 V or 220 V e 1 cuff sensor o size 11 mm is included but can be specified as 9 5 mm or 13 mm when ordering o optional MRI compatible cuff sensors available as add ons 9 5 mm 11mm or 13 mm e 1 restrainer size medium is included but can be specified as small or large when ordering e 1 Pulse cable to MP System or third party A D hardware e
22. se the cursor button or touch screen to move to the pulse with the highest peak and then press the Diastolic button The system will record and display the diastolic blood pressure value You may change your cursor peak positions at anytime during the analysis After measurement is complete press the Save button under Results An automatically generated result code will be displayed at the top of the results section 3 4 Working with Saved Results Previously saved results can be displayed by pressing the Load button under Results Placing the cursor on a desired measurement and pressing OK will load the recorded pressure pulse curves and previously calculated results After loading is complete you can easily evaluate the results and re analyze any measurements 3 5 Turning Off Before turning off the unit make sure that you saved your last measurement Turn off the unit by switching the power button on the rear panel info biopac com Page 11 of 18 NIBP250 User s Guide 3 6 Result File After measurements are performed the pressure and pulse signals including the analysis results can be saved to NIBP internal memory SD Card or USB Memory If you insert any external memory NIBP250 uses it instead of its internal storage Measurements are stored in the directory named as NIBP and assigns an unique name to each i e Rxxxxxx nbp The xxxxxxx denotes the unique number for the measurement i
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