NanoVue™ Plus Spectrophotometer
Contents
1. 2 Sale lease license or other grant of rights to use this material or any material derived or produced from it 3 Use of this material to perform services for a fee for third parties including contract research and drug screening If you require a commercial license to use this material and do not have one return this material unopened to GE Healthcare Bio Sciences AB Bjorkgatan 30 SE 751 84 Uppsala Sweden and any money paid for the material will be refunded All third party trademarks are the property of their respective owners 2008 2010 General Electric Company All rights reserved First published Jan 2008 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan GE Healthcare Bio Sciences AB Bjorkgatan 30 751 84 Uppsala Sweden 28 9301 69 AC 09 20102. chromatography The instrument enables you to characterize oligonucleotide primers by keying in the base sequence up to 66 mer to obtain a conversion factor ug ml molecular weight theoretical absorbance AU umol and theoretical Tm DNA linearity NanoVue Plus shows excellent linearity with double stranded DNA dsDNA from 5 to 6000 ug ml Fig 4 This large linear dynamic range and high reproducibility means that you can proceed to the next stage of your workflow with a high degree of confidence in the data produced by NanoVue Plus Spectrophotometer Calculated value mg ml N 0 1 2 3 4 5 6 DNA mg ml Fig 4 DNA concentration curve 0 5 to 6000 pg ml generated from salmon sperm dsDNA samples measured at 260 nm using NanoVue Plus n 20 replicates mean 1 standard deviation SD Proteins NanoVue Plus Spectrophotometer can be used to determine the concentration of protein samples by a variety of methods including Bradford BCA Lowry Biuret and direct UV methods with a choice of line fit and the ability to run up to 27 standards including replicates These calibration curves can be viewed on the graphical display printed or stored as a method for future use Protein concentration can be determined in the near UV at 280 nm due to absorption by tyrosine tryptophan and phenylalanine amino acids The absorbance at 280 nm varies greatly for different proteins because of their amino acid content th
3. contamination of the sample mechanism thus improving data precision Fig 2 The plate is compatible with a wide range of chemicals including DMSO and DMF e Low sample volumes Reduce sample loss and eliminate the need for dilution by using low volumes of 0 5 to 5 ul for sample measurement The appropriate pathlength for your sample volume is selected automatically unless you opt for manual selection e Fast measurements Read time is typically lt 5 s per sample and you save more time by not having to wash cuvettes or dilute samples Fig 1 NanoVue Plus Spectrophotometer uses a novel drop and measure sample plate which eliminates the need for cuvettes e Convenience and ease of use Large high resolution graphical display enables a quick read of relevant results Calibration curves kinetics or ratio measurements are all displayed at the touch of a button Fig 3 e Flexible analytical performance Full wavelength scan in less than 5 s from 200 to 900 nm with zoom facility peak identification and on peak confirmation Visualization of nucleic acid scans allows impurities to be detected and this is especially useful with RNA samples e Choice of data output Print to an integrated printer optional or to any suitable PC via a USB wireless Bluetooth connection or SD card e Reliability and robust instrumentation Press to read feature reduces the amount of time the lamp stays switched on Optics with no moving parts minimizes
4. the incidence of optical misalignment w E oe ee te ee m a nN pa BSA carry over data mg ml w a 0 D aaa OD Sa 0 5 10 15 20 25 Sample number O water pre BSA sample BSA sample water post BSA sample Fig 2 Sample carry over study using 25 mg ml BSA with successive additions of a water protein water cycle of samples which was repeated 25 times The data showed no significant change in absorbance readings during the study for water sample readings or BSA samples readings thus indicating that there was no sample carry over NanoVue Plus Life Science Protein oN oN 32 Life Science o amp o amp oe Protein UV Protein A280 BCA of eo ot Bradford Lowry Biuret Applications Favourites o o Methods Utilities A B Wavescan ot DNA RE 0 A230 1 53A Sample A260 2 39A A A280 0 94 A A320 0 18A Concentration 110 5 A260 A280 Units A 261 271 318 A260 A280 2 908 1 637 pg ml Abs 0 171 0 204 0 104 Sample 4 C D A 285nmA 0 036 A Fig 3 NanoVue Plus spectrophotometer screenshots showing A Simple interface for easy selection of applications B Protein methods interface C DNA quantitation results and D An example of a wavelength scan The key features of NanoVue Plus Spectrophotometer are summ
5. 0 2116 30 illustra plasmidPrep Mini Spin Kit 28 9042 69 illustra GenomiPhi V2 DNA Amplification Kit 25 6600 30 illustra RNAspin Mini Kit 25 0500 71 illustra HotStart PCR Master Mix 25 1500 01 illustra GFX PCR DNA and Gel Band Purification Kit 28 9034 70 GE imagination at work and GE monogram are trademarks of General Electric Company Amersham Cy CyDye GenomiPhi GFX HyPer illustra and NanoVue are trademarks of GE Healthcare companies CyDye This product or portions thereof is manufactured under an exclusive license from Carnegie Mellon University under US patent number 5 268 486 and equivalent patents in the US and other countries Cy3 UTP or Cy5 UTP Cy3 5 dCTP or Cy5 5 dCTP Cy3 CTP or Cy5 CTP These products are manufactured for GE Healthcare UK Limited by Perkin Elmer Life Sciences under US patent numbers 5047519 and 5151507 The cyanine dyes in the product are manufactured under an exclusive license from Carnegie Mellon University under US patent numbers 5 268 486 and equivalent patents in the US and other countries The purchase of CyDye products includes a limited license to use the CyDye products for internal research and development but not for any commercial purposes A license to use the CyDye products for commercial purposes is subject to a separate license agreement with GE Healthcare Commercial use shall include 1 Sale lease license or other transfer of the material or any material derived or produced from it
6. GE Healthcare Data file 28 9301 69 AC UV Visible spectrophotometers NanoVue Plus Spectrophotometer NanoVue Plus Spectrophotometer Fig 1 is a stand alone instrument with a novel drop and measure sample plate that can be used with a wide range of chemicals NanoVue Plus can be used for the accurate and reliable quantitiation of nucleic acids and protein samples The ability to pipette samples of 0 5 to 5 ul directly onto the sample plate eliminates the need for cuvettes or other sample devices After measurement the sample can either be easily recovered with a pipette or quickly discarded by wiping the sample plate clean for the next measurement NanoVue Plus is pre programmed with a range of preset methods for the quantitation of nucleic acids and proteins using UV or dye intercalation methods Lowry Bradford BCA and Biuret In addition wavelength scanning gives you the flexibility to design your own methods which can be stored in a personal folder for easy access The instrument does not require a computer because it has a direct user interface Fast instrument start up with automatic self calibration means NanoVue Plus Spectrophotometer is always ready to use NanoVue Plus Spectrophotometer offers e Novel gold colored hydrophobic sample plate Save time and effort by directly pipetting your sample onto the sample plate for measurement The hydrophobic coating facilitates sample recovery and minimizes the potential for
7. arized in Table 1 below Table 1 Features of NanoVue Plus Spectrophotometer Feature Specification 200 to 1100 nm Automatic upon switch on Wavelength range Wavelength calibration Pathlength selection Stored methods 90 Automatic with the option for manual selection Spectral bandwidth 5nm Wavelength accuracy 2nm Wavelength reproducibility 0 5 nm Absorbance accuracy Maximum 1 at 259 nm at 0 7 to 0 8 A using uracil Light source Long life stabilized Xenon Detector Twin CCD Array Dimensions 260 x 390 x 100 mm Weight 4 5 kg Power requirements 100 to 240 VAC 10 50 60 Hz 50 VA Note Technical specifications were measured after the instrument had warmed up at a constant ambient temperature 2 28 9301 69 AC Assays Nucleic acids NanoVue Plus Spectrophotometer allows you to measure the concentration and purity of nucleic acids in a variety of units ug ml ng ul ug ul pmol ul and pmol and also to correct for dilution factors where necessary NanoVue Plus displays both individual absorbance values and absorbance ratios 260 280 and 260 230 on the screen along with the sample concentration value A graphical display of the result is also available at the touch of a button The results can be printed immediately with the on board printer option You can examine the UV spectrum 220 to 330 nm for hybridization PCR and sequencing studies or for quantitation of minipreps after isolation by
8. erefore the specific absorption value for a particular protein must be determined NanoVue Plus has in built extinction coefficients for BSA IgG and lysozyme thus allowing you to select the most appropriate for your protein sample or you can input your own extinction coefficients Typical results for BSA and IgG values using the absorbance at 280 nm program are shown in Figures 5 and 6 respectively ra0 9997 Calculated value mg ml 0 10 20 30 40 50 60 70 80 90 100 BSA mg ml Fig 5 Protein concentration curve 0 01 to 100 mg ml generated from BSA protein samples measured at 280 nm using NanoVue Plus n 20 replicates mean 1 SD Calculated value mg ml 0 10 20 30 40 50 IgG mg ml Fig 6 Protein concentration curve 0 025 to 50 mg ml generated from bovine IgG samples measured at 280 nm using NanoVue Plus protein absorbance 280 program with IgG preset option n 20 replicates mean 1 SD Background correction NanoVue Plus provides increased confidence in your results by allowing you to use the background correction feature at 320 nm to compensate for the effects of background absorbance caused by turbidity stray particulates and high absorbance buffer solutions This feature of the instrument is critical because very small samples are susceptible to stray particulates The results are displayed on the screen with other absorbance results for quick evaluation You have the flex
9. ibility to turn the background correction function on or off CyDye You can also measure purity yields and brightness of fluorescently labeled in situ hybridization probes CyDye DNA function holds spectral data for 19 commonly used dyes including Cy 2 Cy3 Cy3B Cy5 and Amersham Hyper 5 and both sample concentration and frequency of incorporation measurements are presented Calibration An accurate pathlength distance is important for the correct functioning of all low volume spectrophotometers We recommend a regular pathlength check as a matter of good laboratory practice The pathlength calibration fluid supplied with the instrument allows for simple checking of the instrument pathlength whenever convenient for added confidence in performance Should re calibration be necessary you can easily and quickly recalibrate NanoVue Plus in your laboratory without the inconvenience and cost of sending the instrument away 28 9301 69AC 3 Drop Measure Done NanoVue Plus provides intelligent performance across all spectrophotometry applications Fig 7 1 Just pipette your sample onto the sample 2 Lower the sample plate head 3 Read your result st Protein UV A260 Sample 2 33A 1 A280 Result 3 8 6 A320 0 000 A Units 3 g ml Fig 7 Operation of NanoVue Plus Spectrophotometer Summary NanoVue Plus Spectrophotometer is an easy to use and re
10. liable instrument for measuring nucleic acid and protein samples Samples of 0 5 to 5 ul can be pipetted directly onto a novel sample plate for measurement and then simply recovered using a pipette In certain cases where sample recovery is not necessary the sample plate can be quickly and easily wiped clean This novel feature eliminates the need for cuvettes capillaries or other sample devices just drop measure and you re done For local office contact information visit www gelifesciences com spectros GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK www gelifesciences com imagination at work Ordering information Product Code No NanoVue Plus with integrated printer US amp CA only 28 9569 63 anoVue Plus with integrated printer Worldwide except US amp CA 28 9569 66 NanoVue Plus print via computer US amp CA only 28 9569 62 NanoVue Plus print via computer Worldwide except US amp CA 28 9569 65 NanoVue Plus with wireless connectivity US amp CA only 28 9569 64 NanoVue Plus with wireless connectivity Worldwide except US amp CA 28 9569 67 anoVue Plus with SD card US amp CA only 28 9569 61 anoVue Plus with SD card Worldwide except US amp CA 28 9569 60 Pathlength calibration kit Worldwide 28 9244 05 Sample Plate Replacement Kit 28 9569 58 Lower Sample Plate Replacement Kit 28 9569 59 Related products Ultrospec 10 Cell density meter 8
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