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1. 2 Enter the Weight then choose the Accept button to return to the Step Definition screen 3 Choose the Set Rate and Concentration button to enter the Set Rate and Concentration screen HARVARD APPARATUS Infuse Rate WIES 04 28 09 1 34 16 PM Enter your information and then press the Enter button green check mark ta accept or press the Cancel button red X to Set Rate and Concentration cancel your settings and return to screen in Concentration 105 985 the previous screen i ml min profile 100 205 nil min Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 10 11 12 13 14 15 16 17 18 Using The PHD ULTRA 62 Select the Infuse Rate Gt should be selected by default as indicated by the active data entry box to the right of the button Enter the desired rate using the keypad or select the Min Rate or Max Rate button If the Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml uul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections Select the Concentration button and enter the concentration of the active ingredient in the infusion fluid in mg ml When all parameters are correctly set on the Set Rate and Concentration screen choose Accept to confirm your settings and return to Step Definition scree
2. 5 Select the Target Time button On the Set Target Times screen enter the desired value using the keypad If the Units of Time are not correct for your application select the Units button to toggle between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon 8 When the Time parameters are correctly set choose the Accept button to return to the Step Definition screen The pump will automatically calculate the flow rate based on the entered Target Volume and Time CONCENTRATION PROFILE The Concentration Delivery profile is used to manage infusion of a defined concentration of drug or other liquid to a set dosage level based on the subject s weight Animal Weight Drug Concentration Doses Time Between Concentration Delivery Doses Pump Operation Profile Dose Time When creating or editing a Concentration Delivery profile you will define the following operating parameters e The Weight of the target subject e The Flow Rate to be used during infusion e The concentration of the drug or other liquid being infused e Either the target dosage or the target infusion time the pump automatically calculates whichever value you do not enter e If you wish to infuse multiple doses to the target you can specify the number of doses along with a delay between dosings Publication 5419 002 Rev J Harvard Appara
3. 8 Choose the Accept button to save your changes and return to the Method Steps Summary screen po NOTE Since the Event Link will cause a jump link to a different method be cautious not to use embedded links in your method programming Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 76 MANAGING EZ PRO METHODS All Preloaded and User Defined Methods are stored in the Method List for ongoing use Over time you may want to manage previously created Methods to keep your Method List easy to navigate In addition the PHD ULTRA allows you to export Method information to an externally connected pump or to a computer that will be used to control operation This section of the Guide describes the file management facilities available on the PHD ULTRA ZL NOTE The method management procedures outlined in this section are not available for Quick Start or Analog Mode Methods Saving A Copy Of A Method Follow these instructions to save a copy of a Method under a new name 1 From the Quick Start or Method Main screen choose the Method Select button to enter the Method Selection screen 2 From the list of available Methods select press once the Method you want to save a copy of then choose the File Options button to enter the File Options screen 3 Choose Save As Copy to access the Method Name Entry screen The current Method Name will be displayed in the te
4. 0 05 0 5 ul 140 ml 1 5 pl min 216 ml min N A Stores all settings N A IEEE 1394 6 pos Type B 15 pin D Sub Connector Option 34 kg 75 Ibs 100 Force Selection 0 9 Stepper Motor Controlled Microprocessor with 1 16 microstepping 12 800 21 5 sec ustep 26 usec ustep 0 18 um min 190 80 mm min 12 30 VDC 5 Input Power at RS 485 12 0 x 8 5 x 4 25 in 30 5 x 21 6 x 10 8 cm 11 75 x 5 5 x 6 5 in 29 8 x 14 0 x 16 5 cm 4 8 kg 10 49 Ibs Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual PHD ULTRA Specifications continued Atmospheric Specifications Operating Temperature Storage Temperature Operating Humidity Storage Humidity Mode of Operation Classification Pollution Degree Installation Category Supplier Name Supplier Address Regulatory Certifications Safety Declarations EMC Declaration Stand Alone 4 C to 40 C 40 F to 104 F 10 C to 70 C 14 F to 158 F See Chart Below 20 80 RH non condensing Continuous Class 1 lI Harvard Apparatus 84 October Hill Road Holliston MA 01746 CE ETL UL CSA WEEE EU RoHS amp CB Scheme ANSI UL 61010 1 Ed 3 CAN CSA C22 2 No 61010 1 Ed 3 IEC 61010 1 Ed 3 CENELEC EN 61010 1 CB Scheme IEC 61326 1 Ed 1 FCC 47CFR 15B Class A Introduction To The PHD ULTRA 12 Satellite RS 485 Remote 4 C to 40 C 40 F to 104 F 10 C to 70 C 14 F to 158 F See Chart Belo
5. 1 position NOTE If a satellite powers up but displays the Main GUI screen instead of e Satellite XX verify that the address of the Satellite is not set to 00 Ba NOTE Each satellite must have a unique address Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 128 Setting Addresses on Satellite Remote Units 1 Connect Satellite Remote unit to PC via a USB cable 2 Launch HyperTerminal 3 Set address using the appropriate Pump Chain Commands Setting Forces on Satellite Remote Units 1 Connect Satellite Remote unit to PC via a USB cable 2 Launch HyperTerminal 3 Set force to desired level in percent using the appropriate Pump Chain Commands BZ NOTE For software versions prior to 1 3 0 the Force level needs to be set from a PC BA via a USB cable With software versions 1 3 0 and newer the Force Level will be sent down to the remote satellite units directly from the master Optional PC to Pump Configurations POWER eee PC CONTROLLED PLIMP ADDR 1 USB POWER STAND ALONE PC CONTROLLED PUMP ADDR 02 STAMD ALONE PC CONTROLLED PLIMP POWER ADOR n3 USE STAND ALONE PC CONTROLLED PUMP ADDR 04 ADDRESSES CAN BE 01 99 PC Control configuration option CONTROL BOX 1 R 485 REMOTE SATELLITE ADB BH ADDR 01 HUE OPTIONAL RS 485 RS 485 OUT CONTROL BOX n R5 485 REMOTE SATELLITE
6. 2 AC Power R 485 RS 495 R5 485 OUT IN OUT IH MUST BE ADDR ADDRESSES CAN BE 01 99 Master Satellite operation using stand alone master units as satellites P optional STAND ALONE Mate 4 MASTER SATELLITE SATELLITE ADOR Di ADDR 07 ADDR 0z AC Power R5 485 OUT R 5 435 R 5 435 IH OUT IH MUST BE ADOR D ADDRESSES CAM BE 01 99 Master Satellite operation using satellite remote units PC optional CONTROL RS 485 REWOTE RS 485 REMDTE R5 485 RBWOTE Mote 1 Box SATELLITE SATELLITE SATELLITE ADOR 00 ADOR 1 ADDR 02 ADDR 03 R 485 RS 425 RS 425 R5 485 OUT IN OUT IH OUT IH MUST BE ADDR ADDRESSES CAN BE 01 99 Gradients using remote system NOTE PC Control via HyperTerminal application Limited command set ye irun wrun stop stp When using stand alone units as satellites do not connect the line cord and leave the power switch in the OFF 0 position Connect units using an RS 485 cable OUT to IN Power up Master Unit Master and Satellites will all power up and Satellites should indicate Satellite XX on their displays where XX represents pump address When using remote units as satellites power switches all operate as indicated turn ON each pump on the RS 485 network NOTE If it is desired to power a Stand Alone unit as a Satellite by using the line cord Ze and internal power supply connect line cord and set power switch to ON
7. Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 6 General Information General Information CONVENTIONS USED IN THIS GUIDE This guide describes basic procedures for operating the PHD ULTRA Syringe Pump including both hardware operations and software operations accessed through the pump s touchscreen display This section discusses important conventions used in this guide Capitalization All menus commands and dialog box option names appear with initial capital letters whether or not they are completely capitalized in the user interface This includes navigation buttons and menu selection buttons selection lists on the touchscreen display This will help you to distinguish these items from narrative or procedural text Terminology Throughout the PHD ULTRA documentation the following terms are used to refer to program elements and the actions that you perform to carry out tasks e Method A Method is the collection of steps and parameters that constitute the operating instructions to the pump while being used to infuse or withdraw liquids e Choose The terms choose and select indicate actions that you perform using the touchscreen interface Choose represents carrying out an action associated with a parameter or navigation button Choose will be used when the choice you make is automatically executed For example Choose the Method Select button automatically brings
8. Using The PHD ULTRA 46 Setting A Time Delay For Method Operation You can set the PHD ULTRA to run a Method at a specific date and time in the future This delayed run function allows you to set up all the parameters for operation then have the pump start unattended at the required time HARVARD APPARATUS 10 28 09 11 45 17 AM Enter your settings to the left and then press the green Run or BD plastic 50 ml 26 594 mm Run Preview button when finished Make sure to set a target volume Set Delay Parameters button on Quick Start screen Quick Start Infuse Withdraw HARVARD APPARATUS 10 27 09 10 14 46 AM Select the desired syringe define steps and then press the green Run or Run Preview button Press the Method Delay button fclock on paper to set a delay Set Delay Parameters button on Method screen Delay Ramp Constant Rate HARVARD APPARATUS Tiel 04 28 09 1 17 31 PM Select format enter date Eus indios EN rm dfyy and time then press the Enter button green check mark to accept or press the Cancel button red X to cancel Set Delay Date and Time vour settings screen SETTING A TIME DELAY FOR METHOD OPERATION 1 From the Method Main or Quickstart configuration screen choose the Set Delay Parameters button to enter the Set Delay Date and Time screen 2 To enter the date select the Date button and type the desired date using the onscreen number pad Select the Date Format but
9. 3 31205 3 52979 11 0552 21 5601 13 6087 31 2204 54 4347 69 9183 106 085 191 171 216 005 ul min ul min ul min ul min ul min ul min ul min ul min ul min ml min ml min ml min ml min ml min ml min ml min ml min ml min ml min ml min ml min ml min Appendices 100 APPENDIX D SYRINGE HOLDER OPTIONS Mounting a 4x140 Multi Rack Clamp Bar Knob Multi Syringe Clamp 2401 071 2401 07 Multi Holder 2401 074 Multi Retainer 2401 070 Lock Washer 2 Bracket Syringe 5174 003 Clamp 2401 050 Clamp 2401 051 Flat Washer 2 5110 038 T Knob 2 5100 059 Dual Syringe Holder 2402 007 4x140 Multi Rack Syringe Holder Assembly Remove the four Set Screws from Syringe Holder using the provided hex key Remove Syringe Clamp bracket from the Syringe Holder by unscrewing knob Place the Multi Holder assembly on the Dual Syringe Holder and attach using four 410 32 x 12 Screws pe 2 er dem Attach Clamp Bar by screwing the Multi Syringe Clamp Knob into the threaded bar attached to the Multi Holder Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 101 Ext Tooth Lockwasher 2 5174 003 Shaft Bracket Clamping 2401 028 Retaining Ring 5007 038 Bracket Anti Syphon 2401 025 Thumbscrew 3 5100 012 Washer Flat 2 5110 038 Knob Bracket Clamping 2401 031 Pusher Bar 2401 067 Spring 3
10. 5 6 ui min Almin 3 Select the Start Rate button it should be selected by default as indicated by the active data entry box to the right of the button Enter the flow rate value desired using the keypad or select the Minimum Rate or Maximum Rate button 4 Ifthe Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections Repeat Steps 3 to 5 for the End Rate by selecting the End Rate button and continuing data entry When all parameters are correctly set on the Set Rate screen choose Accept to confirm your settings and return to Step Definition screen Choose the Set Target Time button to enter the Set Time screen Enter the desired Time value using the keypad 10 If the Units of Time are not correct for your application select the Units button to toggle between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon 11 When all parameters are correctly set on the Set Target Time screen choose Accept to confirm your settings and return to the Step Definition screen BZ NOTE Ramps x 2 seconds from min to max rates may cause motor stalls Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Man
11. aes ole D gt as Low Battery Indication Time Date area will turn red when battery is low See page 119 for instructions on changing the battery HARVARD APPARATUS 04 28 09 1 56 40 PM Enter your settings to the left and press the RUN or Run Preview HA steel 4 851 mm 2 5 ml button Make sure to select the appropriate syringe Quick Start Infusion Only The Time Date area turns 3 525 ml min red when the battery is low plc Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual APPENDIX G SATELLITE BOXES Back view of Satellite box Guide Rods Half Nut Release Pusher Block ead screw Vertical Foot Support Mechanical Stops Front Top view of Satellite box Power LED ANA DIGITAL 1 0 4 K Power Switch RS 2 FOOT e USB SWITCH RS 485 Connectors Appendices 126 Allen wrench Locking Spring for Din Rail Mounting Groove for Din Rail Mounting Syringe Barrel Clamp Syringe Holder Bracket Clamp Run LED User I O Connector Option USB Serial Input For communication from PC For pump to pump communication Close up of Satellite box connections Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 127 Master Satellite Configurations PC optional STAND ALONE STAND ALONE STAND ALONE Mote 1 MASTER SATELLITE SATELLITE ADDR 00 ADDR 01 ADDR
12. prompt Of If 2 Custom diameter mm lt cr gt If prompt Of It code diameter mm lt cr gt This line is repeated for each syringe manufacturer Ift prompt Of If volume unit cr This line is repeated for each syringe size It prompt VALVE Displays or sets the valve state When the valve is set to auto the motor direction determines the valve state Valve defaults to Auto on power up When set to auto OFF Infuse and ON Refill Command format valve on off auto Query response It On cr It prompt Of It Off cr If prompt Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA VER Displays the short version string Command format ver Query response If PHD Ultra cr If prompt VERSION Displays the full version string Command format version Query response If Firmware VEG lt CI gt lt lf gt Pump address lt cr gt If Serial number C lt cr gt It Device ID lt cr gt If prompt RUN COMMANDS IRUN Runs the pump in the infuse direction Quick Start mode only Command format irun RRUN Runs the pump in the opposite direction Quick Start mode only Command format rrun RUN Simulates a k
13. 23 27 5 Cadence Science Inc Formerly Popper amp Sons MICRO MATE Glass Size Diameter 0 25ml 3 47 mm 0 5 3 62 4 82 8 91 8 91 11 71 14 65 19 56 22 7 28 02 35 7 ml Appendices Hoshi Diameter 6 50 mm 9 10 10 00 12 60 15 10 20 45 22 50 25 60 34 00 35 Hamilton Glass All Types Size Diameter 0 103 mm 0 1457 0 206 0 3302 0 343 0 485 0 461 0 729 1 03 1 457 2 304 3 256 4 608 mm 5 151 7 285 10 3 14 567 23 033 32 573 32 573 Becton Dickinson Glass All Types Diameter 4 64 mm 4 64 8 66 11 86 14 34 19 13 22 7 28 6 34 9 Size 0 5 ml Sherwood Monoject Plastic Diameter 4 674 mm 8 865 12 600 15 621 20 142 23 571 26 568 37 948 ILS Glass Size 250 yl 500 1ml Diameter 2 303 mm 3 260 4 606 mm 7 280 10 300 14 567 23 032 32 573 32 573 Top Diameter 6 40 mm 9 30 13 10 15 3 21 0 23 0 29 0 Series 7000 Series 700 Series 1700 Suggested Force Level Settings for Common Syringes NIPRO Size Diameter 1 mllong 6 6mm 1 ml short 4 7 9 0 13 0 15 8 20 1 23 2 29 1 Syringe Type Material Capacity Force Setting Common Manufacturer s Plastic Syringes lt 20ml 50 BD plastic Sherwood Airtite Terumo 20ml 10096 Stainless Steel All 100 Harvard Apparatus Glass Glass lt 20ml 20 Cadence Popper 20ml 30 Glass Plastic lt 1000ul 30 Ham
14. 29 cer P Mechanical Stop Mechanical Stop Syringe fully mounted and locked in place Note mechanical stops set in position to prevent excess travel of pusher block PHD ULTRA CAUTION Be sure the mechanical stops are postioned properly to prevent the plunger from bottoming out on glass syringes Changing the bracket for small lt 30ml vs large gt 30ml syringes 1 Remove the Syringe Barrel Clamp Assembly by completely unscrewing the Syringe Barrel Clamp Knob 2 Unscrew and remove the Retaining Nut and slide the clamp bar off the Syringe Barrel Clamp Knob 3 Flip the bracket over reassemble the Retaining Nut and screw the assembly back onto the pump Large diameter syringe clamping Large vs small diameter syringe clamping Small diameter syringe clamping Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 30 Using The PHD ULTRA PUMP OPERATION OVERVIEW The following diagram provides an overview of basic operation for the PHD ULTRA STEP 1 Select A Method Choose an operating Method which defines the syringe being used the direction infuse or withdraw and rate of flow as well as any dynamic control of external devices If one does not exist you will create a new Method STEP 2 Enter Operating Parameters Enter the operating parameters for each step in your method including specifics about the syringe syringes being us
15. 800 272 2775 Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Declaration of Conformity Manufacturer Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 1388 U S A Phone 508 893 8999 We herewith declare that the following product Product Name PHD ULTRA Syringe Pump Series Model No PHD ULTRA Catalog 70 3xxx To which this declaration relates is in conformity with the applicable EC Directives harmonized standards and other normative requirements Application of Council Directive s 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive 2011 65 EU RoHS Directive Standard s to which conformity is declared Safety EN 61010 1 2012 3rd Edition Emmissions Immunity IEC 61326 1 2005 Ed 1 IEC 61000 4 2 IEC 61000 4 3 2002 IEC 61000 4 4 2004 IEC 61000 4 5 2001 IEC 61000 4 6 2003 IEC 61000 4 8 1993 IEC 61000 4 11 2004 IEC 61000 3 2 2000 IEC 61000 3 3 2002 CISPR11 2003 A1 2004 Group 1 Class A EMC and Safety compliance were evaluated by Intertek ETL Semko Reference test report file numbers 3177745 BOX 001 003 004 005A 006A 007B 3195998 BOX 005a 100602834 BOX 004 004a I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place United States of America Date April 02 2012 Cakes C HARVARD VP Engine
16. Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 56 TO SET UP EDIT A STEPPED RATE PROFILE 1 From the Step Definition screen for a Stepped Rate profile choose the Set Mode button to toggle the Flow Mode between Infuse and Withdraw operation 2 Choose the Set Start amp End Rate button to enter the Set Start and End Rates screen HARVARD APPARATUS Enter your settings ta the left and then press the Enter button Start D ml min End 0 ml min green check mark to accept or press the Cancel button fred X to return to the previous screen Stepped Profile definition screen D secondis 0 Stepts HARVARD APPARATUS Start Rate o o 04 28 09 1 24 36 PM Enter your rates and units and doce JUL then press the Enter button green check mark to accept or E EN press the Cancel button red X Set Start and End Rate ta cancel your settings and return screen 105 985 to the previous screen ml min EN E ES Almin 3 Select the Start Rate button Gt should be selected by default as indicated by the active data entry box to the right of the button Enter the flow rate value desired using the keypad or select the Minimum Rate or Maximum Rate button 4 Ifthe Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of time sec min hr as appropriate
17. The min max values are made available as button selection options on the Set Pulse Rates screens HARVARD APPARATUS _ gt Enter your settings to the left and then press the Enter button Rate 1 0 ml min Rate 2 0 ml min green check mark ta accept or Step Definition screen for press the Cancel button red X a Pulse Rate Profile with to return to the previous screen Volume mode selected D mil O mil 0 Pulse s Ei iv Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 58 TO SET UP EDIT A PULSE FLOW PROFILE 10 11 12 13 14 15 16 17 18 From the Step Definition screen for a Pulse Rate Flow choose the Set Mode button to toggle the Flow Mode between Infuse and Withdraw operation Choose the Set Rates button to enter the Set Pulse Rates screen Select the Rate 1 button it should be selected by default as indicated by the active data entry box to the right of the button Enter the flow rate value desired using the keypad or select the Minimum Rate or Maximum Rate button If the Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml uul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections Repeat Steps 3 to 5 for the second flow rate by selecting the Rate 2 button and continuing data entr
18. h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon 5 If you are changing the Units of Volume on the Units Selection screen select the unit of measure iml ul nl or pl as appropriate then choose Accept to confirm your selection 6 When all parameters are correctly set on the Set Target Volume or Time screen choose Accept to confirm your settings and return to the Quick Start screen Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA USING AN EZ PRO PRELOADED USER DEFINED METHOD The Preloaded Methods and User defined Methods each use the PHD ULTRA s integrated tools that guide users through creation editing of a custom Pump Control Method Pump Control Methods consist of the following set of operating parameters The Method Name e Syringe Information e The set of Pump Operation Profile steps that describe how the pump will control the flow of material for infusion or withdrawal operations e Any additional commands to control the execution of the steps e Any input output I O triggers responses used to control external devices or respond to input signals from external devices Both the Preloaded and User Defined Methods offer the user the flexibility to apply pumping profiles other than continuous rate infusion withdrawal The PHD ULTRA offers a menu of advan
19. infuse and withdraw protocols Preloaded Methods can be edited on Infusion Withdrawal and Infusion Withdrawal Programmable models of the PHD ULTRA They are not available on the Infuse Only model The Preloaded Methods are continuously updated by Harvard Apparatus Engineers so the Methods installed on different pumps may vary For details on specific use of these Methods refer to the applicable instructions in the Quick Start or User Defined Methods sections of this manual EZ PRO User Defined Methods User Defined Methods are created by the user through the application To create a custom Pump Control Method you select New Method After naming the method select the syringe then define the infuse or withdraw operations desired using the available Pump Operation Profiles User Defined Methods can be stored on the PHD ULTRA up to the maximum memory capacity of approximately 800 program steps programmable model only The user can manage the library of stored Methods using convenient touchscreen controls BZ NOTE Method Header takes up 1 step of memory Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 32 RUNNING THE PUMP Controlling The Pump The software provides Fast Forward Infuse and Fast Reverse Withdraw buttons that can be used to manually control the Pusher block and either Withdraw contents into the syringe or Infuse contents from the sy
20. then choose Accept to confirm your selections Repeat Steps 3 to 5 for the End Rate by selecting the End Rate button and continuing data entry When all parameters are correctly set on the Set Rate screen choose Accept to confirm your settings and return to Step Definition screen Choose the Set Time and Steps button to enter the Set Time amp Steps screen Select the Total Time button it should be selected by default as indicated by the active data entry box to the right of the button Enter the desired value using the keypad 10 If the Units of Time are not correct for your application select the Units button to toggle between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon 11 Select the Number of Steps button and enter the desired value using the keypad 12 When all parameters are correctly set on the Set Target Time screen choose Accept to confirm your settings and return to the Step Definition screen Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 57 PULSE PROFILE The Pulse Flow profile is used to create an oscillating infusion or withdrawal profile that varies between a starting flow rate and a second rate that can be either higher or lower than the start rate The flow rate is constant within each segment of the pulsed flow profile and
21. to accept or press End of Run Select screen the Cancel button red X t sbowing tbe preset options and tbe Set Otber Value button return to the previous screen Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 21 Locking the Touchscreen A touchscreen lock function can be enabled to prevent accidental operation or interruption of operation Unlocking the touchscreen requires a password defined by the user HARVARD APPARATUS SETTING THE PASSWORD CHOROS lage 10 27 09 12 00 05 PM To set the Password go to the Software Select the desired setting and Settings screen Press the Set Pump then press the Enter button Password button APE legen green check mark to accept or press the Cancel button red XJ Enter your desired password up to to return to the previous screen x f eee 8 digits and press the enter button Address O TURNING THE TOUCHSCREEN Set Password PowerUp LOCK ON Vi Running Press the Lock button twice to lock the touchscreen HARVARD APPARATUS Methods can be started and stopped Quick Start Infuse Withdraw i while the touchscreen is locked Enter the settings Make sure to Method parameters can not be set a target volume d changed while the touchscreen is BD plastic 60 ml 26 59 mm locked 75 ml min 88 28 ml min Password Lock Button EXE LF i Elfed vi M Rada EMMAMASSEMS T
22. 10 2247 Publication 5419 002 Rev J N A N A 70 2268 70 2252 70 2256 70 2260 70 2264 N A 70 2245 70 2248 Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual N A 10 2253 10 2257 70 2261 70 2265 N A N A 70 2249 N A N A 70 2254 70 2258 70 2262 70 2266 N A N A 70 2250 Appendices 99 APPENDIX C MIN MAX FLOW RATE Nominal Minimum Maximum Flow Rates for Various Syringes Actual Limits will vary depending on syringe manufacturer Minimum Rate Units Maximum Rate Units Syringe Size Syringe ID 0 103 mm 0 1457 mm 0 206 mm 0 343 mm 0 485 mm 0 729 mm 1 030 mm 1 457 mm 2 304 mm 3 256 mm 4 608 mm 4 699 mm 4 851 mm 8 585 mm 11 989 mm 9 525 mm 14 427 mm 19 050 mm 21 590 mm 26 594 mm 35 700 mm 37 948 mm Publication 5419 002 Rev J 1 50000 3 06000 6 12000 16 9800 33 9600 76 7400 153 180 306 600 766 740 1 53126 3 06702 3 18936 3 39900 10 6457 20 7616 13 1046 30 0640 52 4186 67 3288 102 156 184 091 208 005 Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual pl min pl min pl min pl min pl min pl min pl min pl min pl min nl min nl min nl min nl min nl min nl min nl min nl min nl min nl min nl min nl min nl min 1 59133 3 18423 6 36532 17 6471 35 2833 79 7151 159 133 318 423 796 252 1 59021 3 18501
23. 131 Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Quick Guide Quick Guide 10 THINGS YOU NEED TO KNOW TO USE THE PHD ULTRA Since many of our users are already familiar with the operation of a syringe pump we ve created this one page summary of important tips for using the PHD ULTRA 10 BD plastic 50 ml 26 504 mm Run Preview button when Operations Display this section of the screen is used to display lt Message Area this section is used to display the pump operations method information general parameter settings pump name the time date display and the message y and the real time display of data while the pump is running a box which contains operational messages while the pump is running HARVARD APPARATUS sq Run Preview Access the Method Preview 10 27 09 11 31 07 PM screen that shows a Summary of steps and Quick Start Infuse Withdraw operating parameters Enter your settings to the left Method finished Make sure to set a Fast Forward Advances the Pusher Block target volume in the forward infuse direction Fast Reverse Advances the Pusher Block in the reverse withdraw direction Back Used to go back one screen 25 Touch Screen Unlocked Indicates that the Touch Screen is currently unlocked Pressing the button twice locks the Touch Screen A Em Settings Access the settings menu used to adjust Date Time Audible A
24. 37 PM Enter your flow rate and units and then press the Enter button green check mark to accept or press the Cancel button red X ta cancel your settings and return ta the previous screen Set Infuse Rate screen in a Quick Start Infuse Only Method Set Infuse and Withdraw Rates in a Quick Start Infuse Withdraw combined Method Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 42 TO SET THE INFUSE RATE INFUSE ONLY METHOD 1 From the Quick Start or Method Main screen choose the Infuse Rate Select button 2 On the Set Infuse Rate screen enter the desired rate value using the keypad or select the Minimum or Maximum Rate button 3 Ifthe Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections 5 When all parameters are correctly set on the Set Infuse Rate screen choose Accept to confirm your settings and return to the Quick Start or Method Main screen TARGET VOLUME TIME SELECT The Target Volume Time Select function is used to control how long the PHD ULTRA will operate at the set flow rate The run time can be controlled by either pumping to a total volume pumped or the total length of time pumped Quick Start single function Methods Infuse Only or Withdraw Only permit contr
25. 5 009 164 Bracket Anti Syohon 2401 069 Washer Flat 5143 003 Flat Head Screw 2 5137 015 4x140 Multi Rack Pusher Block Assembly 1 Remove Dual Syringe Anti Syphon Bracket by unscrewing the Bracket Clamping Knob completely and removing the Bracket Clamping Shaft 2 Attach the Pusher Bar assembly to the Pusher Block using two flat head screws Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 102 Mounting a 6 10 Multi Rack Clamp Bar Knob Multi Syringe Clamp 2401 072 2401 076 Multi Holder 2401 073 EN i 10 32 x 1 2 Screw 4 5101 087 S J Multi Retainer 2401 075 V4 rA d i pg Bracket Syringe a 5401 550 Lock Washer 2 i l 401 ock Washer l Knob Syringe 5174 003 a Clam j dh WC O a Flat Washer 2 5110 038 Dual Syringe Holder 2400 238 p 2401 051 T Knob 2 5100 059 6 10 Multi Rack Syringe Holder Assembly Remove the four Set Screws from Syringe Holder using the provided hex key Remove Syringe Clamp bracket from the Syringe Holder by unscrewing knob Place the Multi Holder assembly on the Dual Syringe Holder and attach using four 410 32 x 12 Screws pu wee qe d Attach Clamp Bar by screwing the Multi Syringe Clamp Knob into the threaded bar attached to the Multi Holder Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 103 Washer Flat 2 5110
26. AND LUBRICATION DATE AND TIME REMINDERS 1 2 3 4 From the Quick Start or Method Main screen choose the Settings button From the Settings menu choose the Pump Information button to enter the Calibration screen Note that the next date for suggested calibration is displayed on this screen at all times Choose Calibration Reset to set the next suggested Calibration due date based on the current date Note that calibration is suggested once per year so choosing Reset will modify the Calibration due date to one year from the current date The pump will display the total run time since the last reset of the Lubrication timer To reset the time choose the Lubrication Reset button When you are finished choose Accept to return to the Settings menu Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 27 Power Up Running The Power Up Running Setting is designed to allow the pump to restart pump operation automatically after a power outage when operating in a continuous mode The Power Up Running selection is only valid under the following conditions e When operating in a Quick Start Infuse Only or Withdraw Only method e When the pump is running at the time of a power loss e When no target Volume Time is set TO SET POWER UP RUNNING 1 From the Quick Start or Method Main screen choose the Settings button 2 From the Settings menu se
27. Bracket Synnge Clamp 2401 050 knob synnge Glarmopo Des 2401 051 Dual Syringe Holder A AD T D it Microliter Multi Rack Syringe Holder Assembly Remove the four Set Screws from Syringe Holder using provided hex key Remove Syringe Clamp and Knob from the Syringe Holder First assemble the Retaining Bracket to the Micro Holder using the two T Knobs Place the assembled Micro Holder onto the syringe holder and screw in the two Standoff Bars Qe Ie Place the Clamp Bar Assembly onto the Micro Holder by screwing the Thumbscrew Caps into the Standoff Bars Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 107 Retaining Ring 5007 038 Shaft Bracket Clamp 2401 028 Bracket Anti Syphon 2401 025 Washer Flat 2 5110 038 Knob Bracket Clamp 401 031 Ext Tooth Lockwasher 2 5174 003 Bracket Micro Anti syphon 2401 079 Microliter Multi Rack Pusher Block Assembly 1 Remove the Pusher Blocks Retaining Bracket by unscrewing the Bracket Clamping Knob completely and removing the Clamp Shaft 2 Attach Anti Syphon Bracket using the Clamp Shaft and Bracket Clamping Knob Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 108 Syringe Loading Microliter Multi Rack Release Tabs 4 Bracket Clamping Knob 7 Retaining Bracket 9 T Knob Syringe Holder Pusher Block 5 Thumbscrew Cap 10
28. Export Method confirmation PRESS TO CLEAR MESSAGE screen showing successful export TRANSFER A METHOD BETWEEN PUMPS Follow these instructions to transfer a Method between two PHD ULTRA pumps 1 Connect two pumps together via a RS 485 firewire type cable and set the address on the first pump to 00 using the Set Pump Address command in the Settings menu 2 Set the address on the second pump to something other than 00 using the Set Pump Address command in the Settings menu 3 On the first pump from the Method Main screen choose Method Select then choose the Method you would like to export 4 Choose the File Options button then choose the Pump button and enter the address of the pump you would like to receive the Method 5 Choose Export Method to Pump 6 The pump will display a confirmation message in the Message Area on the screen when the export is complete Note that long Methods may take a few seconds to export 7 Before disconnecting the pumps verify that the Method was successfully exported by choosing Method Select from the Method Main screen on the destination pump then browse the available Methods to verify that the exported Method is shown in the menu Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 79 EXPORTING A METHOD TO AN EXTERNAL COMPUTER Follow these instructions to export a Method to an external computer 1 2
29. F MAINTENANCE Maintenance PHD ULTRA series pumps require no special maintenance other than keeping them clean by avoiding accidental spills of pumped material Refer to the chemical compatibility chart below for specific chemical resistance information The two guide rods and the lead screw should be sparingly lubricated periodically every 100 hrs the pump will remind you The guide rods and the lead screw should be lubricated with Super Lube Synthetic Grease provided with the pump To clean the exterior surfaces use a lint free cloth to remove loose dust Use care to avoid scratching the clear display window For more efficient cleaning use a soft cloth dampened not soaked with water an aqueous solution of 75 isopropyl alcohol or a mild detergent Chemical Compatibility Chart Overlay Overlay Chemical Concentration Cover Adhesive Chassis gt gt gt Acetonitrile Methyl alcohol Hydrochloric Acid Bleach Windex Isopropyl Alcohol Dish Soap Motor Oil Simple Green Gasoline Ethylene Glycol Antifreeze Mineral Oil Ammonia Magnalube Superlube Acetone Soda Coca Cola Sulfuric Acid Dimethyl Sulfoxide Sodium Hydroxide Nitric Acid Chloroform UU D UD m50Il5m5l 5IDD5 D r UU 0oU U 0l 5l Il 5 DuUu uUu UO 0 0 DpU 5n 5 5 55 0 5 5 r The chemical resistance data provided is for reference only The data is based on the manufacturer s rec
30. For example to set the infuse rate for pump 12 the command would look like 12irat 3 2 u m If the pump address is one digit it doesn t have to be preceded by a 0 Note that if the USB port is used on a pump with a nonzero address the pump address doesn t need to be used Every line of the response from a pump with a nonzero address is prefixed by the two digit pump address prefixed with a 0 if necessary followed by a colon No colon is used to separate the pump address from the prompt however In the command list below the following convention is used U Required parameter Optional parameter Separator between parameter choices Numeric value without preceding zeros HHH Numeric value with preceding zeros mx A range of values Cr Carriage return Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 83 PHD ULTRA Pump Chain Commands continued It Line feed sp Space prefix Pump address prefix in the format if the pump address is not zero prompt Prompt see below The following prompts are returned after a command is executed The pump is idle gt The pump is infusing lt The pump is withdrawing i The pump stalled T The target was reached If the pump is in poll REMOTE mode there will be no prompts or carraige returns If the pump is in poll ON mode a XON character is added after the prompt If the pump address is nonzero t
31. Pro le Sedecion UO Erg Advanced Options screen E ra showing available command options GO TO STEP The Go To Command is used to instruct the PHD ULTRA to jump to a particular Step in a Method This can be used to help you loop back or jump forward in a Method protocol and can be used in conjunction with I O Events to make the pump respond to a trigger from an external device When defining a Go To jump in a Method the Command targets the Step sequence position and not the function that currently occupies that position For example if you create a Go To jump to Step 4 in a Method currently a Bolus Pump Operation Profile and subsequently move a Constant Rate profile into the Step 4 position the Go To jump will then connect to the Constant Rate operation and not to the Bolus operation TO INSERT A GO TO JUMP From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select once the Method Step that you want the Go To jump to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Set Advanced Options screen pu d 1 D e Choose Go To to insert the Go To Step and view an updated list of all current Steps in the Method HARVARD APPARATUS 04 28 09 1 40 25 PM tep Select the desired step to go ta 100 2 nl min and then press the Enter button green check mark to accept or Advanced Options Go To Step 50 ml min press the Canc
32. Select the Withdraw Rate and enter the desired rate using the keypad or select the Minimum Rate or Maximum Rate button When all parameters are correctly set on the Set Infuse Withdraw Rate screen choose Accept to confirm your settings and return to Step Definition screen Choose the Set Volume per Cycle button to enter the Set Volume per Cycle screen Enter the Volume using the keypad If the Units of Measure associated with Volume are not correct for your application choose the Units button to enter the Units selection screen Select the unit of measure button ml ul nl pD appropriate for your application then choose the Accept button to return to the Set Target Volumes screen When all parameters on the Set Volume per Cycle screen are correct choose the Accept button to return to the Step Definition screen Choose the Set Total Volume or Cycles button to enter the Set Total Volume or Cycles screen Select either Total Volume or Cycles to define how the pump will control pumping activity Total Volume will cycle the pump the correct number of times to deliver the total volume according to the Volume per Cycle setting For example if you set a 10 ml Volume per Cycle and set 100 ml in Total Volume the pump will cycle 10 times If you select Total Volume enter the volume desired using the keypad If you select Cycles enter the total number of Infuse Withdraw or Withdraw Infuse cycles that you wish to perform If the Units
33. Syringe Pump COM7 Windows has successfully updated your driver software Windows has finished installing the driver software for this device USB serial port for PHD ULTRA Syringe Pump 6 Click Close after the software has been successfully installed Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 115 RS 232 Specifications RS 232 9 Pin D Sub Male Baud Rates 9600 19200 38400 57600 115200 Word size 8 Parity None Stop Bits 2 RS 232 Specifications Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 116 Legacy RJ 11 Option Transmit Ground Receive Transmit Ground Receive Pumps with Legacy RJ 11 Option EVER RS 232IN PUMP Ru 11 RJ 431 PUMP RJ RJ 11 PUMP D Sub 9 D Sub9 ADDR OUT Non IN ADDR 1 OUT Non IN ADDR n F F Cable Standard Standard Cat Pin 1 to Pin 1 to 70 4004 Pin 1 Pin 1 Telephone Telephone Cable Cable Cat Cat OR 55 7760 or 55 7760 or 72 2478 72 2478 RS 232 HJ 11 PUMP RJ 11 RJ 11 PUMP RJ11 RJ 11 PUMP D Sub IN ADDR OUT Non IN ADDR1 OUT Non IN ADDR n 9 F to Standard Standard Telephone Telephone Telephone Cable Cable Cable Cat Cat Cat 70 2022 55 7760 or 55 7760 or 72 2478 72 2478 Daisy Chaining with Legacy RJ 11 Option NOTE Pumps 1 n also have RJ 11 telephone jacks NOTE When daisy chaining with RS 232 each pu
34. The 2 5 ml is available with a 1 16 inch SWAGELOK tip only The 8 ml offers SWAGELOK fittings in 1 16 or 1 8 inch sizes All stainless steel syringes are constructed entirely of 316 stainless steel The 20 to 200 ml stainless steel syringes come standard with two Viton O rings for the barrel and one for the tip These Viton O ring seals insures against leakage These syringes are guaranteed to be leak free for pressures up to 750 psi The 2 5 ml stainless steel syringe contains one Perfluoroelastomer O ring seal on the tip and one Bal Seal on the barrel The 8 ml stainless steel syringe contains two Perfluoroelastomer O ring seals and two Teflon O Ring seals on the barrel and a Perfluoroelastomers O ring on the tip These syringes were designed to utilize the high forces available in our syringe pumps to produce pressures up to 7500 psi and 1500 psi respectively All syringes are supplied with inside diameter dimensions for use with Harvard microprocessor controlled pumps and rate charts for use with older classic pumps Replacement Viton O rings are available as are more chemically resistant Perfluoroelastomer O rings High Pressure Stainless Steel Syringes Syringe Size 2 5 ml 8 ml With SWAGLOK Diameter 1 16 inch 70 2269 With Luer Lock Diameter 1 4 inch Diameter 1 8 inch 20 ml to 200 ml 10 2267 10 2251 70 2255 70 2259 70 2263 Replacement Tips Furnished with Sealing O Ring 70 2246 70 2246
35. bigbligbted after a single toucb Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 17 INFORMATION WARNING MESSAGES The Message Area of the touchscreen is used to display helpful instructions for the currently displayed screen It is also used to display error or warning messages to indicate problem conditions in a Method or error conditions during pump operation The Message Area is displayed in yellow when one of these warning messages is displayed An example of an informational warning is shown below HARVARD APPARATUS Gee 04 28 09 11 43 30 AM Withdraw Rate 3 2225 Tbe Set Infusion and Witbdrawal Rates screen showing an out of range Bae setting condition in EN the Message Area The informational warning is displayed n yellow nil min Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual EZ PRO Touch Screen Button Reference The following buttons are part of the PHD ULTRA software aE E3 BS F3 led D uco ke Publication 5419 002 Rev J Settings Access the settings menu used to adjust the following parameters Date and Time Audible Alarms Pusher Block Force Backlighting RS 232 Communications etc Fast Forward Advances the Pusher Block in the forward infuse direction Fast Reverse Advances the Pusher Block in the reverse withdraw direction Accept Used to accept the param
36. create a new method and then press the Enter button green Method Selection screen QuickStart check mark showing a listing of Methods in an Infusion Withdrawal QuickStart Programmable model PHD ULTRA pump QuickStart Method Method From the list of available Methods double tap the desired Method If the Method is not displayed in the list you can use the Scroll Up Down buttons or Page Up Down buttons to advance the list up down as needed Be sure to confirm your selection by pressing the button containing the Method name a second time or choosing Accept You will be returned to the Quick Start or Method Main screen with the Method Select button now displaying the desired Method CONFIRMING METHOD PARAMETERS D With the desired Method displayed in the Method Select button verify that the proper syringe information is set The currently defined syringe settings are displayed in the Syringe Select button If the syringe information is not correct follow the procedure for selecting a syringe defined previously in the Syringe Selection section of this manual Verify that the Rate and Volume Time settings are properly set If changes are required refer to the procedures described later in the Quick Start Method Variables section of this manual With all Method parameters set as desired check to see if the Run button is active If any required parameters are not set or are improperly set e g a value is out of range based on
37. e DI V ow os 10 Connect the pump to the external computer via an RS 232 or USB cable On the computer open the HyperTerminal application and verify that the computer can communicate with the pump On the PHD ULTRA from the Method Main screen choose Method Select then choose the Method you would like to export Choose the File Options button In HyperTerminal choose Transfer Capture Text Enter the File Name that you want to save the Method under then choose Start Choose Export Method to PC The pump will display a confirmation message in the Message Area on the screen when the export is complete Note that long Methods may take a few seconds to export At the destination computer in HyperTerminal when the Method is done transferring the message mend will appear onscreen In HyperTerminal choose Transfer Capture Text Stop Your Method is now saved on the external computer IMPORTING A METHOD FROM AN EXTERNAL COMPUTER Follow these instructions to import a Method from an external computer 1 Sow o m Connect the pump to the external computer via an RS 232 or USB cable and set the Address value to 00 using the Set Pump Address command in the Settings menu On the computer open the HyperTerminal application and verify that the computer can communicate with the pump In HyperTerminal click on the Properties icons and choose the Settings tab on the pop up box that is displayed Click on t
38. entered A Ramp Rate profile can be applied to an increasing or decreasing rate application When creating or editing a Ramp Rate profile you can set the rate in ml ul nl or pl units of volume using sec or h m s hours minutes seconds as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Infuse and Withdraw Rate screens Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA TO SET UP EDIT A RAMP RATE PROFILE 1 From the Step Definition screen for a Ramp Rate profile choose the Set Mode button to toggle the Flow Mode between Infuse and Withdraw operation HARVARD APPARATUS m Enter your settings ta the left and then press the Enter button Start 0 ml min End 0 ml min green check mark to accept or press the Cancel button fred x to return to the previous screen Step Definition screen for a 0 second s Ramp Rate profile 2 Choose the Set Start amp End Rate button to enter the Set Start amp End Rates screen HARVARD APPARATUS Start Rate o E 04 28 09 1 24 36 PM Enter your rates and units and Neate E then press the Enter button green check mark to accept or E EN press the Cancel button red X to cancel your settings and return Set Start and End Rates 105 985 to the previous screen screen El
39. ml Infusion started Press the Target 50 ml Change Current Settings box to Infuse 122 578 ml min change your settings or press the Withdraw 122 578 ml min red Stop button to stop the Quick Start Method Run Flow pump Direction gt screen after a Method has b tarted 29 peers ail 00 00 10 Time Elapsed Time Remaining 00 00 38 Total Inf Volume 19 884 ml Force Level 100 HARVARD APPARATUS 3 stepis Force Level 100 08 24 09 7 33 34 AM Method started Press the red BD plastic 50 ml 26 594 mm Stan Bijctonr ic stop the aun User Defined Method Run Screen after a method has been started Constant Rate Rate SO ml min x Target 5 ml Time Elapsed 00 00 10 Total Volume Dispensed 1 087 ml Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 39 10 To Pause a Method during operation choose the Method Stop button HARVARD APPARATUS 3 steps Force Level 100 08 24 09 7 35 59 AM BD plastic 50 ml 26 594 mm PES ee Peon Run button to resume pumping Delay press the Clear button orange Muibod Pun screen with a arrow to reset or press the Back button to return to the previous User Defined Metbod paused Jl screen on Step 2 Note tbe currently active Step is bigbligbted Constant Rate Rate SO ml min Target 5 ml Time Elapsed 00 00 10 Total Volume Dispensed 1 021 ml 11 To Resume a Method that is paused choos
40. number equal to 3219 35 ul min 4 syringes EIL 4 Select your syringe manufacturer and size as previously instructed Quick Start Infuse Withcdraw p NOTE Possible flow rates and target volumes will change depending on the number of LZ syringes in the gang Previously entered parameters may need to be reset Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 36 HARVARD APPARATUS change Current settings 10 57 09 11 32 29 AM Infuse Withdraw Gang 4 syringes Custom 10 30 mm 100 00 ml Ready to infuse Press the green Target 5 ml Run button to start the infusion Infuse 3219 35 ul min or press the back button to withdraw 15 898 ml min return to the previous screen Run screen showing Direction Mi did a Time Elapsed 00 00 00 roam vone omi oe D gt Force Level 100 NOTE If syringe Gang number 1 the value will not appear on any screen Only displays for Gang 2 Controlling The Pump Using A Stored Method The PHD ULTRA Methods contain the detailed parameters required to control the pump for infuse or withdraw operation This section describes the basic operation of the pump using a stored Method SELECTING A METHOD 1 From either the Quick Start or the Method Main screen choose Method Select to access the Method Selection screen HARVARD APPARATUS 10 27 09 10 08 05 AM Select an existing method or QuickStart
41. numbers symbols choose the 125 button in the lower BEBBIBLDLDDSB me E 4 When you have entered the desired name for your Method choose the Accept button to return to the Method Main screen Your new Method will now be listed in the Method Select button 5 Choose the Syringe Select button and complete the selection of the syringe by following the procedure outlined previously in the Syringe Selection section of this Guide 6 To begin creating Pump Operation Profile Steps choose the Step Definition button to enter the Method Steps Summary screen 7 To add a Step to your Method choose the Add Step button to access the Select Profile Menu screen The available options include a Constant Rate c Stepped e Bolus g Gradient i Advanced Options b Ramp d Pulse f Concentration h Autofill B HARVARD APPARATUS 10 27 09 11 54 21 AM Select the desired step profile or press the Cancel button red X ta return to the previous screen Method Steps Profile Menu screen TTE Advanced Options 8 Choose the Method Step type that you want to add to the Method If you choose a pumping profile e g Constant Rate Ramp etc you will be taken to the Step Definition screen for the selected Method If you choose Advanced Options you will taken to the Select Advanced Options screen 9 Complete the parameter entry applicable to the selected Pump Operation Profile follow the detailed instructions supplied in the Pump Operat
42. or custom defined The min max values are made available as button selection options on the Set Rate and Concentration screen HARVARD APPARATUS 04 28 09 1 38 44 PM Enter your settings to the left and then press the Enter button INF O ml min WD D ml rnin green check mark to accept or press the Cancel button red X to return to the previous screen Step Definition screen for an Autofill cycling profile O ml Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 67 TO SET UP EDIT AN AUTOFILL CYCLING PROFILE 1 10 11 12 13 14 15 16 From the Step Definition screen for an Autofill Cycling choose the Set mode button to toggle between the Withdraw Infuse WD INF and Infuse Withdraw INF WD modes Withdraw Infuse begins by withdrawing into the syringe following by infusing into the target Choose the Set Rates button to enter the Set Infuse Withdraw Rate screen Select the Infuse Rate it should be selected by default as indicated by the active data entry box to the right of the button Enter the desired rate using the keypad or select the Minimum Rate or Maximum Rate button If the Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml uul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections
43. power or is unplugged for a period of time If it is necessary to set the date and time values follow the instructions below TO SET THE DATE AND TIME DISPLAY 1 From the Quick Start or Method Main screen choose the Settings button 2 Onthe Settings screen choose the Date Time Display box to enter the Set Date and Time screen 3 To change the Date select the Date button and enter the Date values using the keypad in the format defined by the date Format button to the right of the Date entry box You can toggle between m d y Month Day Year d m y Day Month Year and y m d Year Month day display by successive selection of the Date Units button 4 To change the Time select the Time button and enter the current time in hh mm ss hours minutes seconds format Select the Time Format button to toggle between AM 24 Hour and PM values for the Time 5 When the Date and Time values are correct choose the Accept button to save your changes and return to the Settings screen Change Logo The PHD ULTRA displays a descriptive name termed logo in the application on the screen The default value for the logo is Harvard Apparatus This name is editable by the user to allow you to customize the pump for your department organization TO MODIFY THE PUMP LOGO 1 From the Quick Start or Method Main screen choose the Settings button 2 Onthe Settings screen choose the Change Logo box to enter the Change Logo screen 3 Using the on
44. syringe selection or other condition the Run button will be inactive grayed out You must have all required parameters validly defined before the Method can be started Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual PREVIEW THE METHOD p Using The PHD ULTRA 57 To Preview a Method press the Method Preview button to enter the Run Quick Start Methods or Method Run Preloaded and User Defined Methods screen ___chenge Current settings Infuse Withdraw SGE 1 03 mm 50 00 ul Target 50 ul Infuse 158 964 ul min Withdraw 156 984 ul min l r Decction le LE 0 000 ul Time Elapsed 00 00 00 Time Remaining 00 00 36 Total Inf Volume O ul Force Level 100 1 stepis SGE 50 ul 1 03 mm Time Elapsed 00 00 06 Total Volume Dispensed 3 981 ul Force Level 100 HARVARD APPARATUS 08 18 09 1 29 18 PM Ready to infuse Press the green Run button to start the infusion or press the back button to return to the previous screen ella jus HARVARD APPARATUS 08 18 09 1 32 03 PM Method paused Press the green Run button to resume pumping press the Clear button orange arrow to reset or press the Back button to return to the previous screen p jas Quick Start Run screen for a Infuse Witbdraw Metbod Metbod Run screen for Preloaded and User Defined Metbods The Run Method Run screens contain a summary of the operating parameters
45. 0 70 4011 70 4012 Appendices 152 Description RS 485 Cable for Pump to Pump Communication 0 5 m RS 485 Cable for Pump to Pump Communication 1 m RS 485 Cable for Pump to Pump Communication 2 m RS 485 Cable for Pump to Pump Communication 30 ft USB Cable for PC to Pump Communication 2 m USB Cable for PC to Pump Communication 5 m RS 232 Cable for PC to Pump Communication 9 pin D sub 2 m Cable Assy Daisy chain Legacy RS 232 RJ 11 2 ft Cable Assy Daisy chain Legacy RS 232 RJ 11 7 ft Line Cord US 115 v Cable for Analog Control Option Adapter PHD Digital I O Adapter D sub 15 to Term Blk Footswitch w Phono Plug Auto Fill Valve Box Low Pressure Auto Fill Valve Box Medium Pressure Auto Fill Valve Box High Pressure Continuous Delivery Valve Box Low Pressure Continuous Delivery Valve Box Medium Pressure Continuous Delivery Valve Box High Pressure Adapter for 25ml 50ml 100ml Hamilton GasTight syringes Hex Key 3 32 Lubricant SuperLube 1cc Stand holds 1 master pump plus 2 satellites RS 485 Adapter See Appendix E External Connections Description Upgrade Infuse Only to I W t Upgrade Infuse Only to Programmable t Upgrade I W to Programmable t Requires Return to Factory T Not available on satellite boxes For additional items such as Syringe Heaters Spill Sensors Temperature Controllers Nano Fluidic Accessories and much more please visit our website www harvardapparatus com or call
46. 0 3007 Push Pull Versions Description Stand Alone System PHD ULTRA w Push Pull Mechanism 70 3008 PHD ULTRA Programmable w Push Pull Mechanism 70 3009 Remote System 70 3305 70 3306 70 3307 Remote System 70 3308 70 3309 Satellite N A 70 3406 N A Satellite 70 3408 N A Appendices Syringe Pump Module N A 70 3506 N A Syringe Pump Module 70 3508 N A Rack Options Catalog Description 70 3020 6 10 Multi Rack Holds up to 10 syringes from 0 5 pl to 20 ml and up to 6 syringes from 30 ml to 60 ml 70 3021 4x140 Multi Rack Holds four 60 ml or 140 ml plastic syringes only 70 3022 Microliter Multi Rack Independently holds 4 syringes from 0 5 pl to 10 ml 70 3023 Anti Siphon Kit Options Catalog Description 70 3030 PHD ULTRA RS 232 RJ 11 Connectors Option 70 3041 PHD ULTRA Analog Control Input Option 0 10v T 70 3034 PHD ULTRA Internal Fan Option 70 3035 PHD ULTRA Digital I O Option Only available with I W and Programmable Models Only available with Programmable Models T Not available on satellite boxes Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Accessories Catalog 70 4000 70 4021 70 4001 70 4020 70 4002 70 4003 70 4004 55 7760 72 2478 5113 001 70 3039 70 4005 70 4006 70 2215 55 010 55 7012 55 008 55 7009 55 7013 55 7007 2401 086 5012 005 70 4013 70 4019 70 4025 Upgrades Catalog 70 401
47. 038 Retaining Rina Shaft Bracket Clamp 5007 038 Bracket Anti Syphon Thumbscrew 3 2401 025 5100 012 Ext Tooth Lockwasher 2 5174 003 Pusher Bar 2401 066 Knob Bracket Clamp 2401 031 Spring 3 5009 164 Bracket Anti Syphon 2401 065 Washer Flat 3 5143 003 Bracket Hold Down 2401 068 Thumbscrew 2 5100 046 Flat Head Screw 2 5137 015 6 10 Multi Rack Pusher Block Assembly 1 Remove Dual Syringe Anti Syphon Bracket by unscrewing the Bracket Clamping Knob completely and removing the Bracket Clamping Shaft 2 Attach the Pusher Bar assembly to the Pusher Block using two flat head screws Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 104 Syringe Loading 4 x 140 amp 6 10 Multi Racks Helease Tabs d Pusher Block Syringe Holder Retaining Bracket Syringe Holder Anti Syphon Bar Pusher Bar 8 Clamp Bar T Knobs Mechanical Stop 9 Infuse Mechanical Stop Withdraw HARVARD APPARATUS Plunger Holder Pulley Cover Allen Wrench Holder Front view of PHD ULTRA with 6 10 Multi Rack showing controls for multi rack syringe loading NOTE Refer to Appendices for additional instructions on assembly of the Harvard 2 Apparatus Multi Rack Accessory 1 Raise or remove Clamp Bar 7 by unscrewing thumb screw 2 Loosen T Knobs 8 and slide the Retaining Bracket 4 on the Syringe Hold
48. 13 SPECIFICATIONS Setting Up The PHD ULTRA With EZ PRO PHYSICAL VIEWS The following diagrams show the important components of the PHD ULTRA pump Analog Control Connector option RS 232 Serial Input RJ11 Connectors for RS 232 optior Power Source When powering a ee Ab pump in a chain Fuse Halder TR MM vno c AA wron through an RS 485 MEM E d cable toggle switch Main Power Switch j o LLL 3 to RS 485 Serial Number Label Universal Power Input Footswitch Input User I O USB Serial RS 485 Connectors switch sold separately Connector Input For pump to pump communication Rear view of the PHD ULTRA pump showing important connections Half nut Release Knobs Pusher Block Guide Rods Syringe Barrel Clamp Vertical Foot lt t Syringe Holder Support RP Bracket Clamp Knobs Mechanical Run Fault LED Stops ova User Interface Touch Screen ole EH I HRHP Lead screw HARVARD APPARATUS Front view of the PHD ULTRA pump showing important components and controls Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 14 POWER CONNECTIONS AND PUMP STARTUP CAUTION Do not connect to firewire devices Damage may occur to pump or device The operating voltage range for the PHD ULTRA is 100 240 VAC 50 60 Hz Use only the specified line cord for this product and make sure line cord is cer
49. 19 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 78 PHD ULTRA Method Export and Import The PHD ULTRA pump permits the daisy chaining of multiple pumps as well as integration with control by an external computer through HyperTerminal To facilitiate operation in these modes the PHD ULTRA application software includes a variety of commands designed to simplify the export import of Methods between the pump and external devices This section outlines the use of these commands NOTE f both pumps have software versions 2 0 0 or higher methods can be transferred BZ via RS 485 f both pumps have software versions 1 3 7 or lower methods can be transferred via RS 485 f one pump has software version 1 3 7 or lower and the other pump has 2 0 0 or higher methods must first be uploaded to the PC and then downloaded to the desired pump using the Method Manager program NOTE Do not attempt to Export Import Methods while the pump is running Do not change screens while Importing Exporting a method NOTE For additional details on the physical interfaces used to connect the PHD ULTRA to external devices refer to the External I O Connections section in the Appendices NOTE Before downloading or transferring a method to the pump verify the number of steps available on the pump information screen Ss a HARVARD APPARATUS 04 28 09 1 44 48 PM The method has been exported
50. 2 From the Settings menu choose the Baud Rate button to enter the Set Baud Rate screen 3 Select the desired preset level value 9600 19200 38400 57600 115200 HARVARD APPARATUS 04 28 09 11 59 39 AM Select a baud rate and then press the Enter button green check mark ta accept or press the Cancel button fred X ta cancel The Set Baud Rate screen your settings and return ta the with the 115200 rate value selected previous screen o 115400 4 When you have selected the desired baud rate choose Accept to save your changes and return to the Settings menu Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Pump Information Setting Up The PHD ULTRA 26 The PHD ULTRA monitors usage and provides convenient reminders for periodic maintenance or calibration You can adjust the settings for the reminders to accommodate your specific operating conditions Harvard Apparatus recommends that system calibration checks adjustments be performed at least once per year on the pump and that lubrication should be performed once every 100 hours of operation Model PHD Ultra Type Programmable Software Version Program Steps Available Calibration Due Date 01 01 12 Maintenance Due 98 hours Pump Address Remote Address HARVARD APPARATUS 01 20 12 01 03 38 PM Reset the Calibration Date or the Maintenance Due as needed Pump Information screen TO RESET THE CALIBRATION
51. A METHOD From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select once the Method Step that you want the Stop Command to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Set Advanced Options screen yes we Iw SD Choose Stop to insert the Stop Command into the Method Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 72 VO EVENT TRIGGERS The PHD ULTRA pump permits easy connection to external I O devices via a variety of interfaces including a built in 15 pin D sub connector The PHD ULTRA software contains simple commands for incorporating I O controls into your pump Methods Integrated commands for control of an external valve multi pump synchronization and general trigger signals are integrated in the software The following procedure describes the use of the Advanced Options I O commands Following the procedure is a description of the selection menu options for each of the I O command types Details for the electrical connection controls and pin out description for the 15 pin User I O Connector are provided in Appendix E of this manual The PHD ULTRA manages trigger events through specific pins on the I O Connector port on the back of the pump The following details define pin logic Triggers are communicated via Pin 4 or Pin 5 e Pump synch
52. Bumper 7 Syringe Holder Retaining Bracket 6 Screw Cap 11 Clamp Bar Assembly Pusher Block Front view of PHD ULTRA showing controls for microliter multi rack syringe loading 1 Squeeze Release Tabs 1 and adjust the Pusher Block 2 to the approximate length of the syringe s 2 Loosen the Retaining Bracket 3 on the Pusher Block 2 by unscrewing the Bracket Clamping Knob 4 3 Loosen the Retaining Bracket 7 on the Syringe Holder 8 by unscrewing the two T Knobs 9 4 Create room for the syringe s by unscrewing the Screw Caps 6 until the Bumpers 10 are at their maximum height 5 Slide the syringe under the Bumper 10 placing the barrel flange within the Syringe Holder s Retaining Bracket 7 and the plunger flange within the Pusher Block s Retaining Bracket 3 NOTE If you are using syringes larger than 1 ml it may be easier to unscrew one of BZ the Thumbscrew Caps 5 and swing the Clamp Bar Assembly 11 to the side making more room to load the syringe s After the syringe s are in place swing the Clamp Bar back over the Syringe Holder 8 and reattach the Thumbscrew Cap 5 Lower the bumpers 10 until they are firm against the syringe s by screwing in the Screw Cap 6 Push the Syringe Holder s Retaining Bracket 7 tight against the barrel flange and tighten into place using the T knobs 9 Then push the Pusher Block s Retaining Bracket 2 tight against the plunger flange and tighten
53. Gradient System and controlling satellite pumps NOTE Master ADDRESS must be set at 00 Satellite ADDRESSES range from 01 to 99 and must be set thru USB While the actual satellite s address is not zero ensure that the Remote Address field under the settings screen is set to O when running a gradient step CAUTION All pumps must use the same syringe size and type If there is a syringe change at Master then same change must occur at all Satellites will not change Force Setting at Satellite Must set thru USB if different f CAUTION For firmware versions prior to 1 3 0 force changed at Master Force is desired at the Satellite s LE NOTE Make all cable connections then power up the satellite pump before powering up the master pump TO SET UP EDIT A GRADIENT PROFILE 1 From the Step Definition screen for Gradient choose Set Combined Flow Rate to enter the Set Rate screen HARVARD APPARATUS 10 27 09 11 54 15 AM Set Addresses and Flow percentage information Gradient Step Definition Screen 0 seconds Ramping Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 64 2 Enter the desired combined flow rate value for all pumps using the keypad or select the Minimum Rate or Maximum Rate button HARVARD APPARATUS QN 10 27 09 11 54 35 AM Enter your rate and units and then press the Enter button green check mark to accept or press the Cancel butt
54. HD ULTRA Syringe Pump Series User s Manual Appendices 113 USB Mating Face USB Type B Female USB VIRTUAL COMMPORT DRIVER INSTALLATION When you connect the PHD Ultra pump to a computer via USB Windows will seek to install a driver for communication The following section details the installation of the Virtual CommPort Driver supplied with the PHD Ultra pump Nie P EFE E WIE 1 ControNpanel System and Security System BA Search Contro Pane Control Panel Home A View basic information about your computer Device Manager Windows edition Remote settings Windows 7 Professional System protection Copyright 2009 Microsoft Corporation All rights reserved Wy Advanced system settings Service Pack 1 et more features with a new edition of Windows 7 System Manufacturyr Dell Model Optiplex 990 Rating m Windows Experience Index Processor Intel R Core TM i5 2500 CPU 3 30GHz 3 30 GHz Installed memory RAM 8 00 GB System type 64 bit Operating System Pen and Touch No Pen or Touch Input is available for this Display Dell support See also Website Orne support Action Center F Computer name domain and workgroup settings Windows Update F Computer name jturcotte 07 W Change settings Performance Information and Tools Full computer name jturcotte 07 harvardapp com Computer description 1 Connect the pump to the computer via USB Open the control panel and sel
55. HD _Ultra_Comrd 2 Enter a name for a New Connection G e PHD_Ultra_Comm then click OK 3 Select the Virtual Comm Port from the Connect using drop down list Click OK df the Virtual Comm Port is not known use Device Manager to find it Instructions on setting up the Virtual CommPort Driver are supplied in Appendix E COMS Properties 4 Setup the Port Settings as shown above and click OK Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 81 PHD_Ultra_Comm Properties ESSI LUE a 5 Verify the Settings are as shown above ASCII Setup 6 Choose ASCII Setup and select Echo typed characters locally then click OK to complete the setup Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 82 PUMP CHAIN COMMANDS The Pump Chain commands allow all pump control information to be managed from an external computer source These commands can also be used to control a series of pumps up to 100 from a single computer interface PHD ULTRA commands are communicated to the pump via the RS 232 or USB port interfaces through a terminal program such as HyperTerminal In using the Pump Chain commands you will need to assign each pump in the pump chain a unique address using the Set Pump Address command in the Settings menu The address
56. Loading Pusti Pull Mec banisi sissisuninsirn rereksane orem omen ern reer 109 APEE Bernal conne OE a E N NM mM EM IE 111 User I O Connector Speci Cat O10 eie tete tette hcec EEREE Ie iii 111 o e d belo RM ree 12 joo X E 113 lider o M OC SAO T TiS EE SEL O DIOE E A cero Sateen A E E EE 116 BR oo EAA A E EA E EE A 117 Remote 7 Gtadient Syste MS eniros dte peeniehehepepepetessises ers ten aC IU Euren eas Ee epe UE 118 Connectine a Remote Satellite PUI ier o pae te re e btri ER Ren HU RES DIE op 118 Setting Addresses on Remote Satellite Units uuum tinte tin rt nl arse rrt 119 Setting the Address on the Conttol Box 7 Master Unitesi 119 df UC Ne ITA MIM nr E ND TA IAM EU RD 121 PER I T T 122 PT TS A E m A 122 Tono OO NE serea eE e AA AERE OE EEEE E AE mU RUNE 123 Dporadio FATRO OI e E E CCN ear E E A E 124 PHD ULIRA Dipli Eror MogsaoE ee eee meee epi nrc Oren e e iA 125 Appendix onele 518 chs esisiini nn str ter DUDEN EUN NNI E Aii 126 Master 7 Satellite Cong ire ale jal sierpe iia E EEE 127 Optional PC 10 Pump Gomi St TIONS sernir iecore ran EE onie E EEEE 128 PPC H REE SAN aaiae A E EREA pEi E a AA 129 App odz d C depre Do FORD DEO kei e E Mir Oh dude sdb enia M e RM EN EE
57. PHD ULTRA Syringe Pump Series User s Guide PHD ULTRA Series MATL 0 3xxx Quick Start Infuse withdraw Enter setti to let press the gn Run or BD plastic 26 594 mm 50 mi Run Pr ton fi sure to set target i AN ias 5 ml min E HARVARD APPARATUS PATENTS PENDING HARVARD APPARATUS Publication 5419 002 Rev J 2 Table of Contents Table of Contents QUICK Guide eR 6 10 Things You Need To Know To Use The PHD ULTRA ssssassiss cnav snnceonsunnanndsianasansiaeueaatsitins 6 EE e E E E A A i ianelcsiseics 7 G nvennons Used Ta THs GUE ocsi ninar arire irr I E REONE 7 Wananiy and cer hes Inora HON sser e EEEE EE EC i 8 nana MDS i ee a ete aso rgrts seers san soe aad lotta eames ene ee E toed ote eain 8 I A MET Y 8 Ropac mie cevl he cies ata E Ges Re 8 S a a E en A eee A A E E E EEE rere 8 OE o o AAEE S E dorado NE 8 e AE E aaa S E E E A EA E AE uate 9 Introduction To The PHD ULTRA Pump c sssssssssssssssessssessessenesessesssaesesaesesaesesaesesaessaesaees 10 Product Overview lt Theoy OF CODOUAEI OD moiran iehi A E A 10 Pan o MP P 10 Se UT PN DS E E E E E E A Mad M tea 11 Setting Up The PHD ULTRA With EZ PRO s sscssssssssessserssessssnesssessserseessersnessonsneassesees 13 a e o E E E er eer TNE E A E E 13 PowerConnecdons and Pamp St EDUD asrni e iii Aeh 14 Remoc Pomp Pio e aC Onn COIE r Er A
58. POR R eo ADDR 01 RS 455 RS 455 OUT IH PC Control configuration option BZ NOTE Control Box must be set at address 0 BZ NOTE Unique comports must be selected for each remote system Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 129 APPENDIX H REMOTE STAND The Remote Stand accessory allows the user to maximize bench space by mounting two satellite remote or PC controlled units with a Master Stand Alone pump at the stand s base DIN Rail Satellite 2 Tilt Adjust Handles Satellite 1 Pump Rest Master Adjusting DIN Rail Height 1 Loosen both Height Adjust Knobs located on the sides of the Pump Rest 2 Slide the Pump Rest to your desired height 3 Tighten both Height Adjust Knobs securely NOTE If adjusting the height with a pump attached loosen one Height Adjust Knob e then use one hand to support the pump while loosening the second Height Adjust Knob NOTE Slot covers provided as stops to prevent the Pump Rests from colliding are Ze located in the slots on the side of the stand The stops can be trimmed to provide more traveling room for the Pump Rests To trim pop out the cover using a screw driver cut to desired length with scissors then snap back into place Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 130 DIN Rail Grooves Locking for DIN Rail S
59. Press Sets the pump to wait for manual input from the user at the touchscreen screen touch TO INSERT AN I O EVENT IN COMMAND From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select once the Method Step that you want the I O Event in to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Select Advanced Options screen Choose the I O Event in button to enter the Event Step Definition Screen Select either Input High Input Low or User Press as appropriate Lowe m i Sy x Choose the Accept button to save your changes and return to the Method Steps Summary screen Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 75 IJO EVENT LINK IN The I O Event Link Gin command is used to have the pump jump to a linked method if the signal is received The external signals are communicated via Pin 2 on the 15 pin I O Connector HARVARD APPARATUS Select which type of event the step will look for and then press Motor Stall the Enter button green check mark to accept or press the Cancel button fred X to return Advanced Options I O Event withdraw to the previous screen Link in Selection Screen The following options are available when setting an I O Event Link Gin command This command requires that you have a method set with the speci
60. RT A STEP REPEAT SEQUENCE IN A METHOD From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select once the Method Step that you want the repeat step to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Set Advanced Options screen Choose Repeat to insert the Repeat Step function into the Method Sy P es co i ouem Select the Step s you would like to repeat from by selecting the Step button twice or selecting once and then choosing Accept HARVARD APPARATUS 05 01 09 1 29 35 PM te M Select the desired step to repeat 182 5 ml min from and then press the Enter button green check mark to Advanced Options Repeat fred X ta cancel your settings bi i rings you to a listing of the available steps in the Method and return to the previous Repeat 7 Enter the number of repeats using the keypad then choose the Accept button to save your changes and return to the Method Steps Summary screen This command creates a loopback to the designated Step in a Method executing the selected Step plus all subsequent Steps up to the point of insertion of the Repeat command For example in the above figure if the Repeat command is set to repeat Step 2 one time the system will loop back to Step 2 and execute both Steps 2 and 3 one additional time Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series
61. Start Update 4 Pump Updater is connected in Update Software mode Open Update File G Pump Update Files PHD ULTRA v2 0 0 srec Ssoloogng00313120456C69746525E737265639F Downloaded file 1319006 bytes COMETE Update complete 7 After the update is complete press Reboot to power Cycle the pump Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 125 PHD ULTRA Display Error Messages Motor Stalled This indicates that the pusher block travel has been impeded This may be caused by the syringe plunger hitting bottom a kink in the tubing occlusion syringe plunger binding or any situation requiring more force to the head of the syringe than the pump is capable of delivering To restart the pump remove the cause of stalling and or increase the force setting then press RUN The pump will continue the interrupted procedure where it stopped Out of Range A value was entered or encountered in a pump program that was beyond the pump s limits Power Failure Notification If power to the pump is interrupted during operation an alarm will sound to alert the user to the interruption When power is restored an onscreen message is displayed to indicate the interruption HARVARD APPARATUS 107 27 09 12 03 41 PM Quick Start Infuse Only ECC TETTE Tbe PHD ULTRA Pump will notify you of a power failure 182 529 ml min with a message when power EINE E g is restored
62. TT E E eee orn Cp rem 14 Navigating The PHID DELBA EZ PRO Oi A edente eaters odode atiii iiie 15 ae PAD URETRA V OES E AEEA rE E OAA AT 15 oceanan An Tem ron A Mena LS a a e er rere 16 Intos5madonmn W anoe Mea GS soikea EE EER EEE EEE er ee ne ere 17 EA PRO TIN Sucon Dutton Bel SECHS nin ann eM a Senne nee 18 piste oerang Adus UNEN Bs ceascebn ean satiN aui OpIUI RUE CAE RIDE HH IECUR NO ERASER E RA 19 DetBlgo to6o cbe OPIBUS uon bpnie nie vera tud On deese Mel cU UM dab 19 Locke Ehe TOUS SOPeeELo docu dote uti odit EAEE A de ce nu e Oa NIS err err 21 BO ENS rane LOI Sona E EE O 22 a a a i DD 23 Sic ibo m 2 TH a ey Fa RPM 24 octne Pump Address V AG sererai i i E Ea 24 Soe dace eg 190 aR E a A E E A EE E EON 25 Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Table of Contents Pan I9 ON ugue sats A EEE Reb EMEND MIN E A E 26 TST Bro RA LN 27 BS stg cece eee ste aon et ys aetna aay gab sob geal shea nen A N DUIS ous baiebobeba nt UE 28 Changing the bracket for small lt 30ml vs large 730ml syringes esses 2 Usine The PHD ULTRA nn ee enn EEE EE eer ee 50 Ivo ci Oates agi ey P 30 Brad SE UN mm 30 hls darse go T 31 EZ TRO Quick Et MAPO eera Uie Dee REA E NEAN 31 EZPRO Preloaded bai eio usce nner aei RISE en et IIT er a a 31 EZPRO Usc
63. URNING THE TOUCHSCREEN Pump Password 01 21 10 09 34 37 AM LOCK OFF Enter the Password up to 8 digits Press the Lock button to bring up the Enter the Pump Password screen Enter your password and press the Accept button ev HARVARD APPARATUS Method Select 04 28 09 12 21 28 PM Quick Start Infuse Withdraw The screen is locked Press the Syringe Select lock button to unlock it BD plastic 60 ml 26 59 mm Infuse Rate Select 75 ml min Withdraw Rate Select e Ox 88 28 ml min Target volume Select Li 60 ml NOTE If you forget your password contact Harvard Apparatus BY Technical Support at 508 893 8999 Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA Setting the Force Limits The PHD ULTRA motor generates up to 75 lbs of linear force in operation This force is sufficient to damage delicate low volume syringes as well as many standard syringes if pumping viscous fluids The application software provides controls to allow you to set a maximum force value calculated as a percentage of the maximum value HARVARD APPARATUS 04 28 09 11 51 09 AM Select an existing percentage or select set other value and then press the Enter button green check mark ta accept or press the Cancel button red X to return to the previous screen amp Filis 209 NOTE For firmware versions prior to 1 3 0 when Satellite units are co
64. User s Manual 70 Using The PHD ULTRA 71 LINK METHOD The Link Command is used to insert a link to another stored Method into the operating sequence of a Method You can Link a Method any place in the Method sequence HARVARD APPARATUS 04 28 09 1 42 33 PM Select the method ta link to and then press the Enter button green check mark to accept or press the Cancel button red X to cancel your settings and return Advanced Options Link Method to the previous screen Command selection screen TO LINK TO A STORED METHOD From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select once the Method Step that you want the Link Command to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Set Advanced Options screen Choose Link to enter the Link Selection screen and display a list of the saved Methods available px HE go Ss cq Double tap the Method that you want to link to in the current Method If the desired Method is not listed on the screen use the Scroll Up Scroll Down or Page Up Page Down buttons to scroll through the Method List Upon selection you are returned to the Method Steps Summary screen STOP The Stop Command is used to instruct the pump to stop operation This Command can be used in conjunction with I O Events to control pump operation based on external events TO INSERT A STOP COMMAND IN
65. a Method 1 From the Method Main screen choose the Step Definition button to enter the Method Steps Summary screen 2 Highlight Select with a single touch the Step that you want to copy 3 Choose the Duplicate Step button The application will insert a copy of the highlighted Step to the bottom of the Method i e the last Step in the Method DEFINING METHOD STEP PARAMETERS This section of the User Guide describes each of the available functions available for use in PHD ULTRA Preloaded and User Defined Methods The information in this section is designed to help you set the individual parameter values in each of the Method Step options and to properly use the Advanced Options commands The information that follows is organized into two sections e Pump Operation Profiles e Advanced Options Commands and I O Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Pump Operation Profiles Using The PHD ULTRA 51 The PHD ULTRA Preloaded Methods and all User Defined Methods use Pump Operation Profiles as part of the Methods controlling the pump These profiles are combined with a syringe selection and any Commands or Input Output triggers to create a complete Method The following sections describe each profile along with instructions for setting up the profile The following Pump Operation Profiles are available on the PHD ULTRA CONSTANT RATE Time Volume STEPPED RATE T
66. aratus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 34 TO CREATE A CUSTOM SYRINGE OPTION 1 From the Quick Start or Method Main screen choose the Syringe Select button 2 From the Syringe Selection menu double tap the Custom Syringe option at the top of the syringe selection menu 3 On the Syringe Dimension Entry screen the Diameter mm entry box should be highlighted by default If it is not choose the Diameter mm button HARVARD APPARATUS Diameter rm 04 28 09 12 23 50 PM Enter your settings ta the left and then press the Enter button green check mark or press the Cancel button red X to return to the previous screen Syringe Dimension Entry screen Volume Enter the diameter of your syringe using the keypad Choose the Volume button and enter the total volume of your syringe If the Volume unit of measure is not correct choose the Unit of Measure button a M E On the Units Selections screen select the Units button appropriate to your syringe then choose Accept You are automatically returned to the Syringe Dimension Entry screen 8 When all parameter entries are correct choose the Accept button to return to the Quick Start or Method Main screen NOTE Each custom method is capable of using and storing a different custom syringe BA but these custom syringe selections are not saved anywhere that can be recalled N CAUTION Be sure to lower the force value when u
67. ard A pinch hazard may exist between the pusher block and end blocks Avoid placing fingers between these points while the pump is running Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qualified service personnel to perform inspection Orient the Equipment Properly Do not orient the equipment so that it is difficult to manage the connection and disconnection of devices Place Product in Proper Environment Review the operating manual for guidelines for proper operating environments Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION Protective Ground Refer to Manual Terminal NOTE 1 Satellite and Remote units are DC powered through the RS 485 cable A Earth ground connection is through this cable for these units Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 9 Introduction To The PHD ULTRA 10 Introduction To The PHD ULTRA Pump PRODUCT OVERVIEW THEORY OF OPERATION The PHD ULTRA Syringe Pump series is a family of high accuracy microliter and milliliter compatible pumps designed for versatile technical use including mass spectroscopy calibration drug and nutritional infusions microdialysis dispensing chromatography and LC HPLC The PHD ULTRA incorporates a microprocessor controlled small step angle stepping motor that drives a lead s
68. as described below Quick Start Run Screen Method type Syringe information Target Volume Summary of Infuse and Withdraw rates as applicable Initial flow direction Total time for the Method Force Level setting Preloaded User Defined Metbod Run Screen Method name Total number of steps in the Method Syringe information Summary flow parameters for the previous step current step and next step to be performed in the method Force level settings To change any of the Method parameters choose the Back button on either the Run or Method Run screen or choose the Change Current Settings button on the Quick Start Run screen Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 38 RUN THE METHOD 9 To start a pump Method choose the Run button While a Method is running the Pump Run LED will be lit The Run Method Run screen will provide real time feedback on the progress of the Method operation as shown below Quick Start Method Preloaded User Defined Methods Current flow direction infuse Total time elapsed for Method ithd withdraw Total Volume Infused across all Method steps Volume withdrawn infused and volume remaining Time elapsed Next Step Information Previous Step Information Time remaining Total volume infused HARVARD APPARATUS Change Current settings 98 14 09 7 30 12 AM Infuse Withdraw HA Steel 28 60 mm 50 00
69. ays the withdrawn time Quick Start mode only Command format wtime Query response lt lf gt seconds lt cr gt lt lf gt lt prompt gt or If 7 cr If prompt DIGITAL I O COMMANDS INPUT Reads and displays the trigger input port status Command format input Query response lt lf gt Low lt cr gt lt lf gt lt prompt gt or lt lf gt High lt cr gt If prompt Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual OUTPUT Using The PHD ULTRA Sets the level on one of the output ports Command format output 1 2 high low SYNC Sets the level on the sync port Command format sync high low INTERNAL COMMANDS STATUS Displays the raw status for use with a controlling computer Command format status The output is in three integer fields and one flag field all separated by spaces and terminated by a carriage return linefeed pair The first integer is the current rate in femtoliters per second The second integer is the infuse time in milliseconds The time has a granularity of 1 millisecond The third integer is the infused volume in femtoliters All three values are for the current direction The flag field consists of six flags Flag one is the motor direction and will be i or I if the pump is infusing and w or W if the pump is withdrawing If the letter is lower
70. bration of the pump N CAUTION For research use only Not for clinical use on patients Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 8 General Information SAFETY INFORMATION Please read the following safety precautions to ensure proper use of your syringe pump If the equipment is used in a manner not specified the protection provided by the equipment may be impaired To Prevent Hazard or Injury Use Proper Line Cord Use only the specified line cord for this product and make sure line cord is certified for country of use The operating voltage range for the PHD ULTRA is 100 240 vac 50 60 Hz See Note 1 Ground the Product This product is grounded through the grounding conductor of the power cord To avoid electric shock the grounding conductor must be connected to earth ground Before making any connections to the input or Output terminals of the product ensure that the product is properly grounded See Note 1 Make Proper Connections Make sure all connections are made properly and securely Any signal wire connections to the unit must be no longer than 3 meters except RS485 pump to pump communication cable Observe All Terminal Ratings Review the operating manual to learn the ratings on all connections Use Proper Fuse Use only specified fuses with product Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Avoid Pinch Haz
71. case the pump motor is idle If upper case the pump motor is running Flag two is the limit switch status If the infuse limit switch was hit i or I is displayed If the withdraw limit switch was hit w or W is displayed If no limit switch was hit or the pump 7 does not have limit switches is displayed Flag three is the stall status and will be S if the pump has stalled Otherwise it will be Flag four is the trigger input state and will be T if high and if low Flag five is the direction port state and will be i or I for infuse and w or W for withdraw Flag six is the target reached status It will be T if the target time or volume was reached and if not Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 96 Appendices APPENDIX A SYRINGE VOLUME DIAMETER REFERENCE TABLE Harvard Apparatus Stainless Steel Size Diameter 2 5 ml 4 851 mm 8 9 525 20 19 13 50 28 6 100 34 9 Air Tite HSW Norm Ject Diameter 4 69 mm 9 65 12 45 15 9 20 05 22 9 29 2 Size 1 ml Becton Dickinson Plasti pak Diameter 4 699 mm 8 585 11 989 14 427 19 05 21 59 26 594 26 594 Terumo Japan Size Diameter 1 mitb 4 70 mm 1 mlvc 6 50 mm 2 5 9 00 13 00 15 80 20 20 23 2 29 2 SGE Scientific Glass Engineering Diameter 100 0 343 mm 0 485 0 728 1 03 1 457 2 303 3 257 4 606 mm 7 284 10 301 14 567
72. ced pumping profiles including e Constant Rate a single continuous rate of flow e Ramped Rate a linearly changing flow rate between a starting and ending flow rate e Stepped Rate an evenly stepped changing flow rate between a starting and ending flow rate e Pulsed Flow an oscillating flow rate varying between a starting and a target rate e Bolus Delivery an infusion operation to occur for a specified volume and time The Infusion Rate is automatically calculated e Concentration Infusion a measured flow rate based on delivery of a specific dosage to a recipient based on the recipient s weight e Gradient Combined flow streams from mutiple pumps into a common output stream e Autofill Cycling continuous infuse withdraw or withdraw infuse operation In addition you have access to a variety of Advanced Options that allow the PHD ULTRA to repeat loop back or jump forward in a Method as well as send signals to external devices or react to signals received from external devices This package of pumping profiles and commands provides users with an extremely broad array of applications that can be easily configured without the use of complex external programming The Preloaded Methods provide a starting set of Methods that define some commonly used application protocols These Methods can be edited and saved under a new name or simply edited to fit your specific requirements Creating a new User Defined Method permits you to leverage al
73. confirm deletion you must select the Delete Step button a second time Move Step Up Moves the currently selected Step in a Method up one position in the Method Order e g Step 3 becomes Step 2 Move Step Down Moves the currently selected Step in a Method down one position in the Method Order e g Step 2 becomes Step 3 Copy Step Creates a duplicate copy of the currently selected Step in a Method and inserts it as the last Step in the Method being created edited Touch Screen Unlocked Indicates that the Touch Screen is currently unlocked Pressing the button twice locks the Touch ocreen Touch Screen Locked Indicates that the Touch Screen is currently locked Press the button and enter password to unlock the Touch Screen File Options Access the File Options menu that is used to manage the list of Methods and to export Method information to an externally linked pump or to a connected computer Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA SYSTEM SETTING ADJUSTMENTS The PHD ULTRA Settings menu is used to control basic system operations This section describes the options available in this section of the application software HARVARD APPARATUS pd 10 27 09 12 00 05 PM Change Logo i Select the desired setting and Audible Pump then press the Enter button Information green check mark to accept or press the Cancel button red X to return to the previo
74. correct for your application choose the Units button to the right of the parameter entry box For the Units of Volume this choice takes you to the Units Selection screen For the Units of Time option this choice will toggle the button between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon 11 If you are changing the Units of Volume on the Units Selection screen select the unit of measure ml ul nl or pl as appropriate then choose Accept to confirm your selection 12 When all parameters are correctly set on the Set Target Volume or Time screen choose Accept to confirm your settings and return to the Step Definition screen RAMP PROFILE The Ramp Rate profile is used to create a linearly increasing or decreasing flow rate for a single pumping mode infuse or withdraw over the course of a defined time period Target Time GP Ramp Rate Pump Operation Profile When creating or editing a Ramp Rate profile you will define three primary operating parameters e The Flow Mode defining the direction of flow infuse or withdraw e The Flow Rate at the beginning and end of the pumping cycle e The Target Time that determines the total length of time over which the ramp will be calculated During a Ramp Rate profile the PHD ULTRA varies the flow rate linearly between the starting rate and ending rate over the full time period
75. crew and Pusher Block Advanced micro stepping techniques are employed to further reduce the step angle to eliminate flow pulsation The pump is engineered to provide flow accuracy within 0 35 and reproducibility within 0 05 The PHD ULTRA uses a touchscreen interface and advanced software to control operation External I O interfaces permit external control via an independent computer or device The application software provides convenient selection of common syringe models from a Syringe Lookup Table as well as insertion of custom syringe specifications The system is then able to calculate the cross sectional area of the syringe selected and calibrate the flow rate and volume accumulation displaying real time operating characteristics via on screen graphics PUMP MODELS The PHD ULTRA Syringe Pump is available in three models designed for different operating environments and varying degrees of operational flexibility e Infusion Only This model supports infusion operations at user definable flow rates and with selectable target volume or time values to control the total infusion volume The entry level Infusion Only model does not include programmable user defined methods e Infusion Withdrawal This model supports infusion and withdrawal operations at user definable flow rates and with selectable target volume or time values to control the total volume pumped for both the infusion and withdrawal portions of a procedure This model provi
76. d Withdraw Rate screens HARVARD APPARATUS Em Enter your settings to the left and then press the Enter button D ml min green check mark to accept or press the Cancel button red X to return to the previous screen Step Definition Screen for a Constant Rate Profile D ml TO SET UP EDIT A CONSTANT RATE PROFILE 1 From the Step Definition screen for a Constant Rate profile choose the Set Mode button to toggle the Flow Mode between Infuse and Withdraw operation 2 Choose the Set Rate button to enter the Set Rate screen 3 Enter the flow rate value desired using the keypad or select the Minimum Rate or Maximum Rate button 4 Ifthe Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections 6 When all parameters are correctly set on the Set Rate screen choose Accept to confirm your settings and return to Step Definition screen Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 53 Choose the Set Target Volume or Time button to enter the Set Volume or Time screen On the Set Volume or Time screen select the Volume or Time button as desired Enter the desired value using the keypad or select the Maximum Volume button 10 If the Units of Volume Time are not
77. denly you feel lost choose the Cancel button or the Back Button Do that enough and eventually you will be back to the Method Main screen or Quick Start screen Watch the Force The PHD Ultra generates up to 75 Ibs of force enough to damage delicate syringes A Force Limit command in the Settings menu allows you to control the maximum force value Get The Message The Message Area on every screen provides a summary of what you are supposed to do and many screens offer warnings or cautions if you do something wrong Set Your Syringe One of the most basic selections on the system is choosing the syringe that is loaded This setting is persistent across all Quick Start Methods the ones we loaded to help you get started fast That means it will be the default syringe choice in every Quick Start Method once you set it up However User Defined Methods save their own syringe settings If you are creating a New Method the default syringe will be the syringe selected for Quick Start Methods Get Connected If you are using the PHD Ultra in conjunction with external devices ones you want to trigger or ones that you will respond to there are commands available to simplify the setup of these connections The Advanced Options in the Method Steps Profiles section of this manual describe these functions Manual Pumping Buttons for manual control of the pump for fast infusion or fast withdrawal action are available on many screens in the application
78. des access to many of the advanced method programming options of the PHD ULTRA but only supports a single stored Method e Infusion Withdrawal Programmable This model supports both infusion and withdrawal operations and can use both simpified pumping profiles or the more advanced pumping profiles and I O settings that permit interactions with external devices In addition to supporting all of the advanced programming tools available on the PHD ULTRA this model also allows users to create and store multiple user defined Methods on the pump NOTE This manual has been written to accommodate Infuse Withdraw Programmable e configurations Where features capabilities are not available in the Infuse Only or Infuse Withdraw basic models we have identified that in the manual PUMP CONFIGURATIONS The PHD ULTRA Syringe Pump is available in the following configurations e Standard Pump This configuration is the most common It consists of a single unit with the syringe pumping mechanism attached to the top of the control box e Remote Pump This configuration is a two piece unit with the syringe pumping mechanism and the control box separated by a 2 meter cable One meter and 0 5 m cables are available for purchase separately e Push Pull Pump This configuration consists of a single unit with the syringe pumping mechanism attached to the top of the control box The pump can hold up to four syringes 2 on either side of the pusher block The pum
79. e display and the message box which contains operational messages while the HARVARD APPARATUS Sear 10 27 09 11 35 03 PM Enter your settings to the left and press the RUN or Run Preview BD plastic 50 ml 26 594 mm button Make sure to select the appropriate syringe C e Navigation Buttons this area of the touchscreen contains the buttons used to navigate through the software menus and Tbe PHD ULTRA Touch Screen Display Tbe screen shown is tbe Quick Start display Quick Start Infuse Only The PHD ULTRA touchscreen with EZ PRO Software allows you to navigate through all menu selections and data entry by gently touching the onscreen buttons with a finger or the tip of a soft non sharp object such as a pencil eraser The software is organized into three main navigational branches e Quick Start Operations Preloaded User Defined Methods e Systems Settings The Method Main or Quick Start screens are the primary home for the applications From those screens you can access all the commands needed to operate the PHD ULTRA as well as the main system settings From anywhere in the applications you can return to the Quick Start or Method Main screens by e Choosing the Cancel or Home Back buttons one or more times until you reach the Method Main or Quick Start screens e Choosing Method Select and making a selection from the Method options available Publication 5419 002 Rev J Harvard Appara
80. e the Run button 12 To Cancel a Method that is paused choose the Clear button The Method is reset to its initial parameters when Clear is pressed 13 Upon completion of a Method operation choose the Re Run button to clear all counters and timers and repeat the Method Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 40 USING AN EZ PRO QUICK START METHOD Quick Start Methods are designed to help you quickly and conveniently use the PHD ULTRA for simple infusion or withdrawal applications Each Quick Start Method requires a minimal number of user entries to operate Basic Operation The following flow diagram describes the use of a Quick Start Method Select Method Type Select Syringe Model OR Create New Syringe Type Quick Start Method operation flow diagram Enter Flow Rate Parameters Infuse Only Withdraw Only or Infuse and Withdraw Enter Target Volume or Time All the required parameter options are accessed from the Quick Start screen The following sections provide a detailed overview of operation for a Quick Start Method STEP BY STEP OPERATION EXAMPLE QUICK START INFUSE WITHDRAW METHOD The following procedure describes operation using the Quick Start Infuse Withdraw Method HARVARD APPARATUS 10 28 09 11 45 17 AM Enter your settings ta the left and then press the green Run or BD plastic 50 ml 26 594 mm Run Preview bu
81. ect System and Security and then System Click Device Manager on the left hand menu gy Device Manager c5 File Action View Help Coen TIRE E 4 Gy jturcotte 07 gt 4M Computer gt xs Disk drives amp Display adapters gt f DVD CD ROM drives fs Human Interface Devices Jungo Keyboards F Mice and other pointing devices K Monitors amp Network adapters ij Other devices Hn Syringe Pump Ports COM amp L Update Driver Software Communica Disable IP Intel R Activ D Processors gt Sound video an Scan for hardware changes lt gt Storage controlli i gE System devices Properties a Universal Serial Bus controllers Generic USB Hub Generic USB Hub Generic USB Hub Generic USB Hub d Generic USR Hub 0 x Launches the Update Driver Software Wizard for the selected device m 3 Uninstall a e mE 2 Two Flash Update Applications will be displayed in the device manager under Other Devices Right click on either of the flash update applications and select Update Driver Software Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 114 j Update Driver Software Syringe Pump Ww r How do you want to search for driver software gt Search automatically for updated driver software Windows will search your computer and the Internet for the latest driver software for your device u
82. ed the type of flow operations you want to use that rate of flow and any details that define the pumping operation STEP 3 Preview Your Method Check all settings using the Method preview function Change any required parameters as necessary STEP 4 Run Your Method Method Monitor real time status detailing the progress of the Method OVERVIEW OF METHODS To operate the PHD ULTRA you will define all the required parameters for withdrawing or infusing liquids through a Pump Control Method The Pump Control Methods will be commonly referred to as Methods A Method defines e Details about the syringe being used for the application e How rapidly you will be dispensing or withdrawing the target fluids e A target volume or target time for the dispensing or withdrawing operation e The pumping pattern you want to employ e g pump at a continuous rate in a ramped fashion using variable rate steps etc e Outbound triggers to external devices e Responses to signals from external devices The PHD ULTRA is shipped with a set of pre installed Methods that are designed to help you to quickly use the pump in common applications These pre installed Methods include Quick Start Methods which are simple infuse withdraw or combination Gnfuse and withdraw Methods that operate in a single step and at a constant rate of flow e Preloaded Methods which use more complex Method programming capabilities to describe commonly encountered appl
83. el button red X screen Choosing the Go To to cancel your settings and return Command brings you to a listing of the available steps in the Method ta the previous screen 6 Double tap the Step that you want to jump to with the Go To Command The system automatically returns to the Method Steps Summary screen which now shows the Go To Step inserted in the Method Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 69 DELAY The Delay Command is used to insert a time delay in a procedure You can program a time delay between 0 2 seconds up to 99 99 99 99 hours 99 minutes 99 seconds TO INSERT A TIME DELAY IN A METHOD aS oe wo em From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight select once the Method Step that you want the time delay to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Set Advanced Options screen Choose Delay to enter the Set Delay screen HARVARD APPARATUS Time Secs 04 28 09 1 40 53 PM Enter time delay and then press the Enter button green check mark ta accept or press the E Cancel button red X to cancel your settings and return to the Advanced Options Set Delay EI EH O previous SCreer screen Enter the desired Time using the keypad If the Units of Time associated with Time are not correct for you
84. ellite Units To change the address of a remote or satellite unit you need to communicate directly to the pump through a USB cable 1 Connect the Remote Satellite unit to a PC via a USB cable 2 Launch HyperTerminal 3 Set address using the appropriate Pump Chain Commands BZ NOTE Remote units are set at a default address of 1 Setting the Address on the Control Box Master Unit The address of the control box or master unit must always be set to 0 zero 1 Onthe pump s touchscreen go to the Settings menu 2 Press the Address 0 Remote X button HARVARD APPARATUS inaade 03 30 10 02 03 28 PM GET tWEP Change Logo Select the desired settings Audible Pump Alarms Information Settings screen showng Baud Rate the Address button 115700 Address O Remote 1 Set Password Running g E Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 120 3 Set the Pump Address to 0 4 Press Remote Address and enter the address of the remote unit 5 Press Accept HARVARD APPARATUS 03 30 10 02 06 42 PM Enter a value between 0 and 99 Pump Address Remote address Set Pump Address screen o E 6 E ea Ld NOTE For gradient systems the Remote Address must also be set to 0 in the Settings Z menu Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 121 APPENDIX
85. er 3 open 3 Adjust the Pusher Block 2 to the approximate length of the syringe s by squeezing the Release Tabs 1 and sliding the Pusher Block into position 4 Loosen the three thumb screws on the Anti Syphon Bar 5 to provide enough room for the plunger flange of the syringe s If using the 6 x 10 rack loosen the two thumb screws on the outside of the Plunger Holder 11 Flip the plunger holder open 5 Place the syringe s on the Syringe Holder 3 ensuring that the barrel flanges are within the Syringe Holder s Retaining Bracket 4 and the plunger flanges are between the Pusher Bar 6 and Anti Syphon Bar 5 NOTE If using fewer syringes than the maximum number of syringes try to place them Z2 symmetrically about the lead screw to present a balanced load 6 Push the Syringe Holder s Retaining Bracket 4 tight against the barrel flanges and tighten into place using the two T Knobs 8 7 Tighten the Anti Syphon Bar 5 using the three thumb screws If using the 6 x 10 rack flip the Plunger Holder 11 back into place and secure by tightening thumb screws 8 Lower or replace the Clamp Bar 7 and tighten thumb screw until Clamp Bar is firm against syringe s 9 Squeeze the Release Tabs 1 and move the Pusher Block 2 to the right until the syringe plunger is located as far into the syringe as it will travel Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Append
86. ering Operations A P P A R A T U S Position Beth Bauman Full Name
87. eters settings on a screen and advance to next screen in the menu Cancel Used to cancel any changes entries on a screen and return to the previous screen in the menu Back Used to go back one screen Set Delay Parameters Used to set the date and time values for delayed operation This permits the pump to start automatically at a preset date and time oet Delay Parameters Indicates the Delay Timer has been set The pump will start automatically on the day time selected Run Preview Access the Method Preview screen that shows a summary of steps and operating parameters Run Resume Used to Run or Resume a Method He Run Clears counters timers and runs the selected method from the beginning otop Used to stop running a Method Clear Clears the counters timers Minimum Value Allowed Used to select minimum value allowed Maximum Value Allowed Used to select maximum value allowed zi E B3 dg EJ Lj Im Ll Setting Up The PHD ULTRA 18 Page Down Used to page down in a display list Page Up Used to page up in a display list ocroll Up Used to scroll up in a display list Scroll Down Used to scroll down in a display list Insert Step Used to insert a new Step in a Method while editing creating the Method The new Step is inserted after the Step that is currently selected Delete Step Used to delete the selected otep in a Method while editing creating the Method To
88. ey press of the run button located in the lower right corner of the screen Command format run SIOPTISTIP Stops the pump Command formats Stop stp Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 89 WRUN Using The PHD ULTRA Runs the pump in the withdraw direction Quick Start mode only Command format wrun RATE COMMANDS CRATE Displays the current rate that the motor is running at A valid response is returned only in dynamic situations while the pump is running Quick Start mode only Publication 5419 002 Rev J Command format crate Query response If Infusing at xl xxx lt cr gt Ift prompt Of lt lf gt Withdrawing at xI xxx lt cr gt It prompt DIAMETER Sets or displays the syringe diameter in mm Quick Start mode only Command format diameter syringe diameter Query response If 7 mm cr If prompt Sets or displays the syringe count Quick Start mode only Command format gang syringe count Query response If syringes lt cr gt If prompt Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 90 Using The PHD ULTRA 91 IRAMP Sets or displays the infusion rates while ramping Quick Start mode only Command format iramp start rate start units end rate end units ramp time in seconds Que
89. fic actions steps you want to perform if the event occurs so that you can choose the Method name being linked by this command Input High to Low Will link to the desired method upon a logic low input signal falling edge Input Low to High Will link to the desired method upon a logic high input signal rising edge e User Press Will link to the desired method upon a manual input Motor Stall Will link to the desired method upon a Motor Stall event e Withdraw Will link to the desired method upon a Withdraw activity e Infuse Will link to the desired method upon an Infuse activity TO INSERT AN I O EVENT LINK IN COMMAND From the Method Main screen choose Step Definition to enter the Method Summary screen Highlight select once the Method Step that you want the I O Event Link in command to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Select Advanced Options screen Choose the I O Event Link Gn button to enter the Event Link Step Definition Screen px HE m oe iw qe Select the desired Event type that you want to have the event associated with from the options available Input Low Input High User Press Motor Stall Withdraw Infuse 7 After selecting the desired option in the Event Link screen choose Accept to access the Method Steps Summary selection screen Choose the Method name to jump to To use this command you must have defined a linkable Method
90. from a reservoir for refilling the syringe to the target infusion subject NOTE Pin 8 of the 15 pin I O Connector adapter on the rear of the PHD ULTRA will e actuate to control a Harvard Apparatus Valve Box used in conjunction with an Autofill Pump Operation Profile Cycles or l Total Volume Autofill Cycling Pump Operation Profile Withdraw Time Volume When creating or editing an Autofill Cycling profile you will define the following operating parameters e The Flow Mode defining the cycle Infuse Withdraw INF WD or Withdraw Infuse WD INF e The Flow Rates to be used during infuse withdraw operations e The infusion volume to be delivered during each pump cycle e The total volume to be delivered during all cycles or the total number of Infuse Withdraw or Withdraw Infuse cycles that you want the pump to execute For example if you want to pump 100 ml using a 10 ml syringe the pump will execute 10 Infuse Withdraw or Withdraw Infuse cycles depending upon the chosen flow mode During an Autofill profile the PHD ULTRA operates at a constant pump rate during the infusion and withdrawal stages although the rate can be different for each direction i e infuse vs withdraw When creating or editing an Autofill Cycling profile you can set the rate in ml ul nl or pl units of volume using sec min or hr as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected
91. g The PHD ULTRA 85 CMD Sets or displays the command set The pump is capable of operating in the ULTRA command set or the legacy Model 22 or PHD 44 command sets Valid sets are ultra 22 and 44 Command format cmd command set Query response lt lf gt Ultra lt cr gt If prompt Of lt lf gt 22 lt cr gt lt lf gt lt prompt gt or If 44 lt cr gt If prompt CONFIG Sets or displays the pump configuration Command format config config designation value BZ NOTE After changing the configuration the pump must be power cycled DELMETHOD Deletes a method stored in the pump Command format delmethod method DIM Sets or displays the backlight level in percent Valid range is 0 to 100 Note that specifying O will turn the backlight off so that the screen will not be visible Command format dim 0 100 Query response lt lf gt lt cr gt If prompt ECHO Sets or displays the RS232 USB echo state Valid states are on or off Command format echo on off Query response If OFF cr It prompt Of It ON lt cr gt If prompt Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 86 FREE Displays the number of free method steps left on the disk Command format free Query response lt lf gt
92. g binary gradients you do not have to set the address for the third BA pump Verify that the calculated values for this non existent pump are equal to zero NOTE To unassign a pump from a gradient step set pump address to zero in the Set A Pump Number screen Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 65 12 Choose the Set Time amp Steps button Enter the total amount of time and the number of steps desired and then choose Accept HARVARD APPARATUS 10 27 09 11 55 37 AM Enter your total time and number of steps and then press the Enter button green check mark to accept or press the Cancel button red X ta cancel and return to Gradient Time and Type the previous screen Entry Screen NOTE For a Constant rate E or Hamp v profile choose O steps For a Stepped profile l select the number of steps desired NOTE When running a gradient step ensure that no unused pumps are included in e the pump chain Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 66 AUTOFILL CYCLING PROFILE The Autofill Cycling Profile is an automated Infuse Withdraw or Withdraw Infuse step that operates at constant but separately defined flow rates for the withdraw and infuse operations The Autofill profile is designed to work in conjunction with an autofill valve that controls the switching of flows
93. ge define steps and then press the green nr Run ar Run Preview button Press Sherwood 140 ml 38 4 mm the Method Delay button clock Method Main screen on paper to set a delay showing a sample Method with 10 steps Delay Ramp Constant Rate meam 4 gt gt Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual SYRINGE SELECTION Using The PHD ULTRA 33 In order to accurately control the flow rate or volume delivery of the pump the PHD ULTRA requires that each method include accurate setting of the syringe diameter and volume All Methods Quick Start Preloaded User Defined require a syringe definition either by selecting from the installed syringe specifications list or by defining a new Custom syringe in the application The built in Syringe Lookup Table provides a convenient lookup function that allows users to select from a wide variety of syringes from major manufacturers including Air Tite Becton Dickinson Hamilton Harvard Apparatus Cadence Science SGE Scientific Glass Engineering Sherwood Monoject and Terumo This Guide also includes a reference table of major syringe diameter and volume parameters in Appendix A Syringe Volume Diameter Reference Table The following parameters must be set for every syringe selection Syringe Diameter mm e Syringe Volume ml or pl high viscosity liquids you should adjust the Force value in the Se
94. ge Pump Series User s Manual 109 Appendices 110 y v iP mdr i FN MO ia PHD ULTRA showing syringes installed on both sides of the Pusher Block HARVARD APPARATUS NOTE For Syringes lt 10ml you will need to remove the Thumb Nut 8 and then e remove and flip over the Syringe Clamp 3 Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual APPENDIX E EXTERNAL CONNECTIONS User I O Connector Specifications All Inputs are pulled high to 5 Vdc through a 10k resistor All Outputs are at TTL Logic Levels Pulse duration should be 0 1s minimum Trigger 1 Out Trigger 2 Out Footswitch In Sync Out Event In Direction Out Biecnowin Valve Out t Run Indicator Run Indicator or Voltage NOTE For remote pumps I O connection must be made at the remote box Not the control box Appendices 111 Pin Assignments 1 Direction control input Rising edge sets pump to infuse Falling edge sets pump to refill 2 Trigger Input Event trigger falling edge triggers a program event 3 Footswitch Input or Timer Settable to work as Momentary switch closure to ground or TTL Logic Low falling edge toggles between states run stop Starts on rising edge stops on falling edge Starts on falling edge stops on rising edge 4 Trigger 1 Output TTL Logic Output Default Low Control thru method or serial comm 5 Trigger 2 Outpu
95. he ASCII Setup button In the Line Delay field enter 500 This will set a 500 millisecond delay after each line is transferred during the import process Click OK twice to return to the HyperTerminal Main Menu screen In HyperTerminal choose Transfer Send Text File Select the Method that you want to import to the PHD ULTRA pump and click Open Before disconnecting verify that the Method was transferred by choosing Method Select from the Method Main screen at the destination PHD ULTRA pump then browse the Method menu to confirm that the new Method is now saved at the pump NOTE The Harvard Apparatus Method Manager program provided on the ULTRA CD can be used to simplify the import export process for programmable mE pumps Please refer to the Method Manger manual for instructions Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 80 EXTERNAL PUMP CONTROL This section of the PHD ULTRA manual describes the control of the pump using an external computer device Setting Up HyperTerminal HyperTerminal is a Windows application designed to support the external control of devices such as the PHD ULTRA pump through an RS 232 or USB connection The following instructions describe the configuration of the HyperTerminal application 1 Select Start All Programs Accessories Communications HyperTerminal Connection Description lt P
96. he pump address is prefixed to the prompt without a colon In the following list of commands The pump address is prefixed to every response line followed by a colon lt If gt lt prompt gt is the response unless otherwise noted Error messages Error messages are displayed if the entered command cannot be executed for some reason The error message will take up two lines with the first line being the message type and the second line describing the error itself The second line may be up to 80 characters long COMMAND ERRORS Command errors are displayed when the command is unrecognized entered in the wrong mode or the state of the pump keeps the command from executing G e using the IRUN command if a limit switch is active The command error has the following format lt lf gt Command error lt cr gt lt lf gt lt sp gt lt sp gt lt sp gt error message lt cr gt It prompt ARGUMENT ERRORS Argument errors are displayed when a command argument is unrecognized or out of range The argument in question will be displayed except in the case of missing arguments The argument error has the following format If Argument error bad argument cr It sp sp sp error message cr It prompt Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 84 System commands ADDRESS Sets or displays
97. hods stored on the PHD ULTRA match your requirements you can create a new Method and store the Method for use The following flow diagram outlines the basic steps you will follow to create a new Method Choose Tubing Select E Miete Mall Tube Select Screen Choose Step Definition Y Method Selection Method Steps Summary Screen Y Add Step Y New Method Step Definition Screen New User Defined Metbod creation workflow Name Method Complete Parameter Entry Choose Accept Add More Steps Steps Summary Screen TO CREATE A NEW USER DEFINED METHOD 1 From the Method Main or Quick Start screen choose the Method Select button to enter the Method Selection screen 2 Select New Method then choose Accept or press New Method again Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 48 3 On the Method Name screen type the name of your Method up to 15 characters using the onscreen keyboard Choose the Symbols Numbers button 123 to display the list of available numbers and symbols that can be included in the Method Name To switch the keyboard back to letters mode choose the Letters button ABC HEN 105 CHAR MAX Ie e E E BS ECT HE LT New Method Name Entry A E LJ F G Ll EH K GG screen showing letter keys To view
98. ications NOTE What Methods are available on your pump depends on the specific model you p are using An Infusion Only model supports only the Quick Start Infuse Method The Infusion Withdrawal model supports all Quick Start Methods and one custom Method The Infusion Withdrawal Programmable model supports all Methods described in this manual In addition users of the Infusion Withdrawal Programmable model PHD ULTRA can quickly create custom Methods to support more complex dispensing scenarios including e Multiple infusions e Ramped or stepped infusion rates e Periodic dispensing loops e Combination infuse and withdraw profiles e External system control or response to external signals Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 31 METHOD TYPES EZ PRO Quick Start EZ PRO Quick Start Methods are designed for simple infusion or withdrawal applications pumping at a constant flow rate for either a predetermined time period until a preset volume is pumped or running in continuous operation until stopped The PHD ULTRA is supplied with the following pre installed Quick Start Methods depending on the model of PHD ULTRA pump you are using Infuse Only All Models The Infuse Only Quick Start Method is designed to pump liquids from the syringe at a set flow rate Users can set a Target Volume or Time period for operation If no Target Volume or Ti
99. ices 105 10 Using the Allen Wrench located in its holder in the rear of the Pulley Cover 12 loosen the Infusion Mechanical Stop 9 and slide it to the left until it is in contact with the Pusher Block 2 Use the Allen Wrench to tighten it in this position 11 Squeeze the Release Tabs 1 and move the Pusher Block 2 to the left until the plunger is located just to the left of the maximum volume allowable Use the Allen Wrench to loosen the refill Mechanical Stop 10 and slide it to the right until it is contact with the Pusher Block 2 Tighten the Withdraw Mechanical Stop 10 and then return the Allen Wrench to its holder 12 HARVARD HARVARD 4 x 140 Multi Rack 6 10 Multi Rack Multi Rack Configurations BZ NOTE When multiple syringes are loaded it may be difficult to manually move the A Pusher Block in order to set the Mechanical Stops Instead use a single empty syringe to measure the proper lengths and set both the Infuse and Withdraw Mechanical Stops first Then use the above procedure to load your syringes Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 106 Mounting a Microliter Multi Rack Clamp Bar Assembly 2400 290 Spacer Micro Clamp 2 5015 035 T Knab 2 5100 054 Spring Washer 2 Micro Holder prng prier ta J 2 2401 080 Micro Petainer 9401 08 Oa a A A o o a
100. ilton SGE lt 5ml 50 5ml 100976 Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 97 Appendices 98 APPENDIX B STAINLESS STEEL SYRINGES Cat No Product Replacement Parts 20 to 200 ml 9013 089 9013 090 9013 091 9013 092 9013 109 72 2472 72 2473 72 2474 72 2475 72 2616 72 2617 Chemraz O Ring 20 ml Chemraz O Ring 50 ml Chemraz O Ring 100 ml Chemraz O Ring 200 ml Chemraz Tip Seal O Ring sizes 20 200 ml Replacement Viton O Ring 20 ml pkg of 10 Replacement Viton O Ring 50 ml pkg of 10 Replacement Viton O Ring 100 ml pkg of 10 Replacement Viton O Ring 200 ml pkg of 10 Replacement Viton Tip Seal O Ring sizes 20 200 ml pkg of 20 Stainless Steel Plunger Button to Adapt Syringe for Use with PHD 22 2000 HVP with Triple S Clamp Required for 50 ml and 100 ml syringes only Harvard High Pressure Stainless Steel Syringes Harvard offers a complete line 2 5 to 200 ml of Stainless Steel Syringes intended for high pressure applications with good resistance to most aggressive liquids Wetted parts are 316 stainless steel or Viton Syringes are available in 2 5 8 20 50 100 and 200 ml sizes with removable replacement tips The 20 to 200 ml stainless steel syringes offer SWAGELOK fittings in 1 16 1 8 and 1 4 inch sizes A luer lock fitting is also available The tips are interchangeable for 20 to 200 ml syringes
101. ime Volume BOLUS DELIVERY Time Volume GRADIENT Pump Operation Profiles available on the PHD ULTRA Publication 5419 002 Rev J RAMP RATE PULSE FLOW Pulses Time Volume CONCENTRATION DELIVERY Animal Weight Drug Concentraton Doses Time Lag Dose Time AUTOFILL niusa Cycles or Total Volume Flow Rate Withdraw Time Volume Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 52 CONSTANT RATE PROFILE The Constant Rate profile is used to run the pump at a defined flow rate and for a specific pumping mode infuse or withdraw up to a target volume or for a defined time period Target Time or Total Volume Point Constant Rate Pump Operation Profile Time Volume When creating or editing a Constant Rate profile you will define three primary operating parameters e The Flow Mode defining the direction of flow infuse or withdraw e The Flow Rate to define the rate of uptake or dispensing e The Target Volume or Target Time that determine how long the pump will operate at the set rate When creating or editing a Constant Rate profile you can set the rate in ml ul nl or pl units of volume using sec min or hr as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Infuse an
102. inition button to enter the Method Steps Summary screen 2 Highlight select with a single touch the Step for which you want to change the order 3 Choose the Move Step Up or Move Step Down button as required to move the Step into the position that you want it executed NOTE If there are Method Commands e g Go To that link to the Step moving Steps By can result in unexpected results as the Command may be linked to the Step number You should always recheck all Method Commands after adding deleting or moving Steps in a previously created Method DELETING A STEP FROM A METHOD 1 From the Method Main screen choose the Step Definition button to enter the Method Steps Summary screen 2 Highlight Select with a single touch the Step that you want to delete 3 Choose the Delete Step button The application will display a warning message in the Message Area asking you to confirm your deletion request HARVARD APPARATUS 04 28 09 1 22 38 PM 5 Seconds 100 2 ml min 106 ml min 50 ml min showing confirmation message on the screen 4 To confirm the deletion choose the Delete Step button again The Step is removed and you are returned to the Method Steps Summary scree Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 50 DUPLICATE A STEP IN A METHOD Use this procedure to insert a copy of a Step with all its associated parameter settings into
103. ion Profiles Preloaded and User Defined Methods section later in this Guide If you selected Advanced Options follow the instructions supplied in the Incorporating Advanced Options Into a Method section later in this manual e NOTE When setting a Target Time for a method step the minimum length is 0 5 seconds Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 49 10 When you have completed all parameter entries on the Step Definition screen or completed the required entries for Advanced Options choose the Accept button to save your changes and return to the Method Steps Summary screen 11 Repeat items 7 10 in this procedure to include additional Steps in the Method e NOTE When adding a Step to a Method the Step is added after the currently selected Step i e the Step that is highlighted in purple 12 When you are done adding all required Steps to the Method choose the Back button to return to the Method Main screen Modifying a Preloaded User Defined Method Once you have created a Method or have reviewed a Preloaded Method you may have a need to modify the structure of the Method i e the types of Steps and Commands included not just changes in the operating parameters for a specific Step The following procedures will help you to efficiently modify a stored Method CHANGING THE ORDER OF STEPS IN A METHOD 1 From the Method Main screen choose the Step Def
104. is listed in a series of buttons beneath the Method Name HARVARD APPARATUS 04 28 09 1 13 35 PM Select desired step You can add 3 285 ml min 3 335 nl min delete move up down or set time delay Press the Back button ta return to the previous screen 3 285 ml min Step Definition Screen sbowing tbe list of Steps currently defined for tbe Metbod To review details for any individual Step in the Method select the Step you wish to review then press the Step button again to enter the Step Definition screen for that Step p NOTE To edit the existing profile press the left side of the step button To replace this step with a new profile press the right side of the button on the picture To edit the parameters for any Pump Operation Profile follow the instructions supplied in the applicable Pump Operation Profile description contained in the Setting Method Parameters section later in this Guide To return to the Method Steps Summary screen when finished reviewing or editing the Step parameter details choose the Accept button to save your changes or choose the Cancel button to cancel any changes you made When you are finished reviewing the operating details for all Steps choose the Back button to return to the Method Main screen 10 To preview the Method choose the Preview button or to run the Method choose the Run button Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual
105. isDebtued Netus nne eee ene ee 31 Parna TROT e aa E A E A E E E E NU ai bout 52 Conroe The TU T 32 basic NTA Oa A E A AA du PBEUAS 32 Viewing Corrent Method PAEREDE ECTS arripiens tren Te teen ete neem EEEE rr errr I IE MU 22 acca states gee ete 25 DUHBOC Gan 9 DEEABORBI rrea E s vis pm e see ou acim Er ieii 54 Controline The Pump Using A Stored M lhOd co vughebtade di atex bina eam as ieni EEE 36 Denpan LZ PRO Can rndiioi RE teenage scence eoleence 40 Pi c OPE O teaser 40 Quick Stm Method SOEIADIE Sarr Senne rior E E 41 Infuse Rate Select Withdraw Rate Sel6ef a esetsuteseterseer urere eta dero eve vene eus vutueo dt rbec edet 41 Tacer Volume Time SE e EU oss esqes iuba epdtetdiep erede sd aU Uds dE 42 Using an EZ PRO Preloaded D ser Defined Method rere enini nibns 43 Preloaded Uset Defined Method Workflow e eeeeeeeeeeeeeeeee entente nne 44 setot A Mme Delay For Method Opera euin n rT nme em ter eneE tT a rr UM ION 46 Cireanne an EZ PRO Now Method osccacscsesecesescsqretaceciepoiceuszeecesectuaceecvensateataceseensenaorenncaceeeceseesices 47 Modifying a Preloaded User Detined Methoden ete teiie eret nire dee ieiens 49 Changing the Ordet ot Ste psdn a MCI etastaiscaussntarcasutesiinns andote loved doceas eed aE 49 Deride a Prona N Odara e 49 IB e eire a e AE whi a o a E T A E E renee on Te tre 50 Dengine Meihod Ste DoD EUNIG CES orinte
106. isplay the range limits Command format wrate max min lim rate rate units Query response lt lf gt xI xxx lt cr gt If prompt Of If xI xxx to xl Xxx cr If prompt ZZ NOTE For commands requiring rate units use the following format m u n p h m s Ex m m milliliter minute n s nanoliter second VOLUME COMMANDS CIVOLUME Clears the infused volume Quick Start mode only Command format civolume CTVOLUME Clears the target volume Quick Start mode only Command format ctvolume CVOLUME Clears both the infused and withdrawn volumes Quick Start mode only Command format cvolume CWVOLUME Clears the withdrawn volume Quick Start mode only Command format cwvolume Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 92 Using The PHD ULTRA 93 IVOLUME Displays the infused volume Quick Start mode only Command format ivolume Query response lt lf gt xl lt cr gt If prompt SVOLUME Sets or displays syringe volume Quick Start mode only Command format svolume syringe volume ul mI Query response Ift 7722 ul lt cr gt If prompt Of If 777 ml cr If prompt TVOLUME Sets or displays the target volume Quick Start mode only Command format tvolume target volume volume units Query re
107. l the options available on the PHD ULTRA Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Preloaded User Defined Method Workflow Using The PHD ULTRA 44 The following flow diagram describes the use of an existing Preloaded or user Defined Method Select Method Select Syringe Model OR Create New Syringe Type Review Method Steps Pump Operation Profiles Enter Pump Operation Profile Parameters Validate Commands and I O Operations Preloaded User Defined Method workflow As with Quick Start Methods the EZ PRO software provides the user with a variety of prompts and warnings to help guide the setup of methods For example if a pump Method incorporates steps that exceed the capacity of the defined syringe the system will prompt you that parameters are out of range The system also prompts users if required values are not entered NOTE The pump will check the validity of parameters entered for each Step in the BA Method but will not review the Method in its entirety to verify that it is logical and can be fully executed Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 45 STEP BY STEP OPERATION EXAMPLE USING A PRELOADED OR USER DEFINED METHOD The following steps describe operation using a Preloaded or User Defined Method In the following description we are assuming the use of a Meth
108. larm Pusher Block Force Backlighting etc Navigation Buttons g Set Delay Parameters Used to set the date this area of the touchscreen contains the and time values for delayed operation This buttons used to navigate through the software permits the pump to start automatically on menus and to start stop pump operations the date time selected Navigation Buttons Review the Touch Screen Button Reference on Page 18 of this manual to familiarize yourself with the onscreen navigation commands Once you know the buttons you ll be able to navigate freely in the system Select and Choose Menus on the PHD ULTRA that require selection from a list use a persistent double touch to select an item That means that you will touch the desired item once to highlight it then a second time to confirm your choice Most other commands only require that you touch one time to choose that function Home The Home screen in the PHD ULTRA software is the Method Main or Quick Start screen This screen displays the currently selected Method along with its current parameter settings To change Methods choose the Method Select button Running A Method To run a Method choose the Run button It will point left or right depending on whether the first operation is a Withdraw left or Infuse right Step If all the required parameters for the Method are not set the Run button will be inactive grayed out Lost If you start navigating and sud
109. last group and work backwards a NOTE When removing power be sure to remember that power removed from a stand alone unit will also cause power to be removed from all RS 485 powered units deriving power from it hs NOTE Power may be removed from any group of pumps except the first group without affecting communications down stream X NOTE If a chain of pumps with mixed software is desired pumps with software versions 1 3 7 or lower can be returned to manufacturer for a hardware update bs Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 118 Remote Gradient Systems Remote systems consist of a control box and remote unit attached via an RS 485 cable Gradient systems consist of up to two pumps satellites or stand alones attached to a master pump via RS 485 cables Control Box Remote Pump RS 485 R5 485 IEEE 1394 L Cable Addr X Remote System Master Pump RS 425 BERE 38 IEEE 1594 IEEE 1394 Cable Cable Gradient System Connecting a Remote Satellite Pump When a remote pump is connected using an RS 485 cable to a control box or stand alone ULTRA a pump control button representing the remote unit will be displayed on the Method Main or Quick Start Main screen In this button is a number that represents the remote s address Gradient systems with two satellite units attached through an RS 485 cable will have two but
110. lect the Power Up Running button The checkbox on the Power Up Running button will be checked to indicate that the function is on To toggle the function off select the button again 3 When the Power Up Running button is set as desired choose Accept to save your changes and return to the Quick Start or Method Main screen p NOTE Counter Timer information is reset when power resumes Counter Timer BA information prior to power loss is lost Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA SYRINGE LOADING 1 Release Tabs 2 Pusher Block 3 Syringe Holder Block 4 Retaining Bracket Pusher Block 5 Retaining Bracket Syringe Holder Block 6 Syringe Barrel Clamp Knob 7 8 Bracket Clamping Knobs 9 Mechanical Stop Infuse 10 Mechanical Stop Withdraw 11 Pulley Cover Allen Wrench Holder 12 Syringe Barrel Clamp Front view of PHD ULTRA showing important controls for syringe loading 1 Adjust the Pusher Block 2 to the approximate length of the syringe s by squeezing the Release Tabs 1 and sliding the Pusher Block 2 into position 2 Loosen the Retaining Brackets 4 5 on both the Pusher Block 2 and Syringe Holder Block 3 by unscrewing the Bracket Clamping Knobs 7 8 Lift the Syringe Barrel Clamp 12 by unscrewing the Syringe Barrel Clamp Knob 6 3 Place the syringe s on the Syringe Holder Block 2 En
111. me value is entered the pump runs continuously until the Mechanical Stop is reached or the user presses the Method Stop button e Withdraw Only Infusion Withdrawal and Infusion Withdrawal Programmable Models The Withdraw Only Quick Start Method is designed to withdraw liquids into the syringe at a set flow rate Users can set a Target Volume or Time period for operation If no Target Volume or Time value is entered the pump runs continuously until the Mechanical Stop is reached or the user presses the Method Stop button Infuse Withdraw Sequence Infusion Withdrawal and Infusion Withdrawal Programmable Models The Infuse Withdraw Quick Start Method is designed to cycle through an infusion operation followed by a withdraw operation The user can set separate flow rates for both the infusion and withdrawal sequences In addition the user must set a Target Volume for the Method e Withdraw Infuse Sequence Infusion Withdrawal and Infusion Withdrawal Programmable Models The Withdraw Infuse Quick Start Method is designed to cycle through a withdraw operation followed by an infusion operation The user can set separate flow rates for both the withdrawal and infuse sequences In addition the user must set a Target Volume for the Method EZ PRO Preloaded Preloaded Methods have been designed by Harvard Apparatus engineers to accommodate commonly employed pump control delivery protocols These applications include infuse withdraw and combined
112. mp must be self powered If a pump in the RS 232 daisy chain is powered down all pumps down stream from it will lose communications e e Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 117 PUMP TO PUMP CONNECTION RS 485 CAUTION Do not connect to firewire ports on a PC Damage may ocur to pump and or PC Mating Face RS 485 In Signal RS 485 Out Signal Notes PWR IN PWR OUT 430Vy Protected by resettable fuse GND GND RS 485 A TIED TO PIN 4 100 Q w to RS 485 B TIED TO PIN 3 ene ten nisin TIED TO PIN 6 RS 485 A TIED TO PIN 5 RS 485 B RS 485 In Out IEEE 1394 Sockets USBor USBor PUMP RS 485 RS 485 PUMP RS 485 RS 485 PUMP RS 232 IN IN RS 232 D subg ADDR OUT keer IN ADDR 1 IEEE 1394 ADORN D Sub 9 Cable Cable F F Cable Daisy chaining via RS 485 RS 485 Dasiy Chain Connection Options e f all pumps have software versions 2 0 0 or higher 99 pumps in chain e f all pumps have software versions 1 3 7 or lower 99 pumps in chain e f mix of pumps with software version 1 3 7 or lower and 2 0 0 or higher maximum of 5 pumps in chain and 1 RS 485 adapter catalog 70 4025 required NOTE When daisy chaining with RS 485 at minimum every fourth pump must be self powered i e another stand alone unit This can be affected by Force Selection X NOTE When applying power to pumps in a daisy chain apply power to the
113. mware v2 U Pump address Oo Disk space free 790 method steps po NOTE Itis recommended that you save your methods periodically by uploading them to a PC Unit won t power up Verify that the Power Source Switch on the rear of the pump is set to AC IN if powering through linecord or RS 485 if powering in a chain AC IN POWER SOURCE RS 485 Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 124 Upgrading EZ PRO Software 1 Upload the latest software version to your desktop format is filename srec 2 Disconnect all I O devices and then connect the pump to PC using a USB cable 3 Press settings button then press Upgrade Software button twice to enter Boot Loader Mode BZ NOTE To exit bootloader mode without upgrading the software power cycle the pump NOTE Prior to upgrading users will need to install the PHD ULTRA USB driver as p well as the bootloader driver file PHDUltra Bootloader inf Reference USB Virtual Comport Driver Installation 4 From the CD provided with the pump open the ULTRA series Pump Updater Application 4 Pump Updater Pump is connected in Update Software mode G Pump Update Files PHD ULTRA v2 0 0 srec solgo000313120456C6974652E737265639F File Read Successfully press Start Update 5 Click Open Update File and browse the file previously saved on your desktop and click Open 6 Click
114. n Choose the Set Dose or Duration button to enter the Set Dose or Duration screen On the Set Dose or Duration screen select either the Dose button if you wish to enter the target dosage value in which case the PHD ULTRA will calculate the required pumping duration or Duration if you wish to infuse for a specific time period in which case the PHD ULTRA will calculate the delivered dose As appropriate enter either the Dose in mg ml or the Duration using the keypad If the Units of Time associated with Duration are not correct for your application select the Units button to toggle between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon When all parameters on the Set Dose or Duration screen are correct choose the Accept button to return to the Step Definition screen Choose the Set Doses amp Time Between Doses button to enter the Set Doses amp Time Lag screen HARVARD APPARATUS o o 04 28 09 1 35 46 PM TEE NM Enter the dose and time infa and o o o ieee then press the Enter button green check mark to accept or E press the Cancel button red X Set Doses and Time Lag ta cancel your settings and return semen mi Concentration aie ancl yr sts o es EM Select the Doses button it should be selected by default as indicated by the active data entry box to the right of the button Enter the desired numbe
115. nless you ve disabled this feature in your device installation settings gt Browse my computer for driver software Locate and install driver software manually 3 Select Browse my computer for driver software o Qj Update Driver Software Syringe Pump Browse for driver software on your computer Search for driver software in this location Harvard Apparatus USB CDC inf V Include subfolders gt Let me pick from a list of device drivers on my computer This list will show installed driver software compatible with the device and all driver software in the same category as the device 4 Click Browse and navigate to the pump drivers folder located on the CD supplied with the pump Select the file Harvard Apparatus USB CDC inf Make sure the Include Subfolders box is checked and then click next rE kel Windows Security Se Windows can t verify the publisher of this driver software Don t install this driver software You should check your manufacturer s website for updated driver software for your device Only install driver software obtained from your manufacturer s website or B disc Unsigned software from other sources may harm your computer or steal information E ERE RR Y See details 5 Ifa warning message regarding the driver publisher is displayed choose Install this driver software anyway W il Update Driver Software USB serial port for PHD ULTRA
116. nnected to Master Force Setting only affects force limit on Master Satellites retain last set value Default force 5096 TO ADJUST THE MAXIMUM FORCE SETTING 1 From the Quick Start or Method Main screen choose the Settings button 2 From the Settings menu choose the Force button to enter the Set Force Level screen 3 Select the desired preset level value or use the Set Other Value button to enter a custom force level 4 If you select Set Other value enter the desired force setting as a percentage value 1 100 then choose Accept to confirm your setting 5 When you have selected or entered the desired force value choose Accept to return to the Settings menu Suggested Force Level Settings for Common Syringes Syringe Type Material Capacity Force Setting Common Manufacturer s Plastic Syringes lt 20ml 50 BD plastic Sherwood Airtite Terumo gt 20ml 100 Stainless Steel All 100 Harvard Apparatus Glass Glass lt 20ml 20 Cadence Popper 20ml 30 Glass Plastic 1000yl 3096 Hamilton SGE lt 5ml 50 5ml 10096 Suggested Force Level Settings for Common Syringes Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 22 Setting Up The PHD ULTRA 23 Setting the Time and Date The PHD ULTRA displays the date and time on each screen in the user interface Built in battery backups maintain the date and time even if the pump loses
117. od that is already saved on the PHD ULTRA Note that if you make any changes to the Method s operating parameters and choose Accept on the page where the changes are made the changes automatically become part of the stored Method for future use 1 6 From the Method Main screen the Method you wish to use should be displayed in the Method Select button If the Method is not displayed select the correct Method using the procedure outlined in Running The Pump Choose the Syringe Select button to enter the Syringe Selection screen Follow the instructions in the Syringe Selection section of this Guide to complete the selection of a syringe from the built in Syringe Lookup Table or to create a new syringe When finished choose the Accept button to save your changes or choose the Cancel button to cancel your changes and return to the Method Main screen Review the Pump Operation Profile Steps described in the Step Definition button Note that the software will display the first three Steps in the Method in the main button area If the Method uses more than three Steps the last Step in the Method will be displayed in the box beneath the Step Definition button To review the Method Steps in detail or to update modify any of the stored Pump Operation Profile Parameters choose the Step Definition button to enter the Method Steps Summary Screen The name of the Method is listed at the top of the screen and the complete list of Steps
118. of Measure associated with Volume are not correct for your application choose the Units button to enter the Units selection screen Select the unit of measure button ml uul nl pD appropriate for your application then choose the Accept button to return to the Set Target Volumes screen When all parameters on the Set Total Volume or Cycles screen are correct choose the Accept button to return to the Step Definition screen NOTE Using maximum rate may not be appropriate during the Withdraw stage of an A Autofill profile since air can be drawn into the syringe or a high vacuum pressure can be produced depending on the syringe and or tubing being used or the fluid viscosity Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 68 Incorporating Advanced Options Commands and I O Into A Method PHD ULTRA Methods can incorporate a variety of commands that expand the versatility of the system by permitting convenient looping chaining of events sending signals to external devices or accepting inbound signals from external devices These Advanced Options controls are described in this section of the Guide To access the Advanced Options choose Advanced Options from the Select Profile screen HARVARD APPARATUS e 04 28 09 1 39 16 PM Delay Select desired function or press the Cancel button red X to Li link gD in return to the previous screen Method Step
119. olling the pump for either target time or volume Quick Start composite Methods Infuse Withdraw or Withdraw Infuse only permit control by Target Volume When setting this parameter you can choose between the following units of volume ml ul nl or pD and the following units of time sec h min sec The system automatically calculates a Maximum Volume value based on the syringe selected or custom defined for the Method HARVARD APPARATUS SUME 04 28 09 1 10 59 PM er 5 IS Enter your target volume or time pate and then press the Enter buttan green check mark to accept or f press the Cancel button fred X Set Target Volume Time for to cancel your settings and return setting pumping duration to the previous screen in a Quick Start Infuse Only Method NOTE For Infuse Withdraw Methods this parameter only permits the setting of the A Target Volume parameter TO SET THE TARGET VOLUME OR TIME 1 From the Quick Start screen choose the Target Volume Time Select button 2 Onthe Set Target Volume or Time screen select the Volume or Time button as desired 3 Enter the desired value using the keypad or select the Maximum Volume button 4 If the Units of Volume Time are not correct for your application choose the Units button to the right of the parameter entry box For the Units of Volume this choice takes you to the Units Selection screen For the Units of Time option this choice will toggle the button between sec seconds and
120. ommendations and is not a guarantee of chemical compatibility for your test conditions Wipe any spills immediately A Satisfatory D Severe Effects B Fair Minor Effects not tested C Poor Moderate Effects Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 122 Fuse Replacement Key Information 1 Make sure the power cord is disconnected from the main supply before servicing the fuse 2 Use only Type 3AG 1 4 x 11 4 amp 250 volt Type T time delay fuses Harvard Apparatus Part No 5146 037 or equivalent Turn off power and remove power cord from power module Use a straight blade screw driver to pry open the access door Remove the fuse holder and then remove the fuses from this holder as shown in figure below Replace fuses Battery Replacement and then replace the fuse holder 1 Remove screw and swing cover to the side 2 Slide battery out from under clip 3 Install Lithium Coin Battery 3V 16mm CR1620 Harvard Part No 5155 288 or equivalent by sliding under clip with positive side facing out CAUTION Use only same type amp rated battery Observe polarity when installing yz NOTE Does not apply to Remote Satellite units Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 123 Troubleshooting Infusion Accuracy To ensure infusion accuracy always use new
121. on red X E to cancel your settings and return Combined Flow Rate Entry EI E 6 220 975 to the previous screen screen ml min 212 792 nl min 3 Ifthe Units of Rate are not correct for your application choose the Units button On the Units Selection screen select the unit of measure ml ul nl or pl and the unit of time sec min hr as appropriate then choose Accept to confirm your selections 5 When all parameters are correctly set on the Set Rate screen choose Accept to confirm your settings and return to the Step Definition screen Choose the Set Percentages button for pump 00 Set the Start and End Percentages for pump 00 then choose Accept Choose the Address button and then set the address of the second pump Choose Accept to return to the Step Definition screen HARVARD APPARATUS Pump number o 10 27 09 11 55 17 AM Enter the number of the pump and then press the Enter button green check mark to accept or press the Cancel button red X E to cancel your settings and retum Gradient Pump Address EI E 6 to the previous screen Entry Screen 9 Choose the Set Percentages button for the second pump and enter your desired percentage then press the accept button 10 Choose the second Address button and then set the address of the third pump Choose Accept to return to the Step Definition screen 11 The pump will calculate the remaining percentages for the third pump NOTE If performin
122. p can simultaneously infuse and withdraw the exact same amount e Satellite Pump Units Satellite units consist of a syringe pumping mechanism and control electronics but do not have a user interface They are powered and controlled from a master stand alone PHD ULTRA via the RS 485 cable Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual PHD ULTRA Specifications Accuracy Reproducibility Syringes Minimum Maximum Flow Rate Minimum 0 5 yl syringe Maximum 140 ml syringe Display Non Volatile Memory Connectors RS 232 RS 485 USB 1 0 amp TTL Linear Force Max Drive Motor Motor Drive Control Motor Micro Steps per one rev of Lead Screw Step Rate Minimum Maximum Pusher Travel Rate Minimum Maximum Power Dimensions Control Box Remote Box Weight Publication 5419 002 Rev J Stand Alone 0 25 0 05 0 5 ul 140 ml 1 5 pl min 216 ml min 4 3 WQVGA TFT Color Display w Touch Screen Stores all settings 9 pin D Sub Connector IEEE 1394 6 pos Type B 15 pin D Sub Connector 34 kg 75 Ibs 100 Force Selection 0 9 Stepper Motor Controlled Microprocessor with 1 16 microstepping 12 800 21 5 sec ustep 26 usec ustep 0 18 um min 190 80 mm min 100 240 VAC 50 60 Hz 50 W 0 5 A fuse 12 0x 8 5 x 7 0 in 30 5 x 21 6 x 17 8 cm N A 4 5 kg 10 Ibs Introduction To The PHD ULTRA 11 Satellite RS 485 Remote 0 25
123. pring Mounting Locking Spring Hight Adjust Knob Tilt Adjust Handle Adjusting DIN Rail Tilt 1 Loosen both Tilt Adjust handles by turning clockwise To increase the handles range of motion pull the handle out and twist counter clockwise then allow the handle to click back into place 2 Once both handles have been loosened tilt the DIN Rail to your desired angle and tighten into place using the Tilt Adjust Handles NOTE Ensure that the DIN Rail is tightened securely using the Tilt Adjust Handles to LZ prevent any movement when the pump is attached Mounting a Pump 1 The rear side of a Satellite Remote pump has a Groove with a Locking Spring for DIN Rail mounting Position the pump s top groove and locking spring over the top edge of the DIN Rail 2 Push the top of the pump down against the spring while pushing the bottom of the pump back until the bottom groove locks under the DIN Rail Dismounting a Pump 1 Holding both sides of the pump push down on the top of the pump to depress the Locking Spring 2 Tilt the bottom of the pump out to release the bottom Groove from the DIN Rail 3 Lift the pump off of the DIN Rail Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual APPENDIX I ORDERING INFORMATION Standard Version Description Stand Alone System PHD ULTRA Infuse Only 70 3005 PHD ULTRA Infuse Withdraw 70 3006 PHD ULTRA Infuse Withdraw Programmable 7
124. r application select the Units button to toggle between sec seconds 0 2 seconds minimum allowable value and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon You can enter a value up to 99 99 99 When your Time entry is correct choose the Accept button to insert the Delay Step in the Method and return to the Method Steps Summary screen Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA REPEAT STEP The Repeat Command is used to repeat Steps in a procedure a defined number of times To use this Command you will tell the application what Steps you want to Repeat and how many times you want them repeated The Repeat Step command creates a jump back to a specific Step in a Method that executes the defined Step plus all following Steps up to the Repeat command i e it creates a Method loop When defining a Repeat jump in a Method the Command targets the Step sequence position and not the function that currently occupies that position For example if you create a Repeat jump to Step 4 in a Method currently a Bolus Pump Operation Profile and subsequently move a Constant Rate profile into the Step 4 position the Repeat jump will then connect to the Constant Rate operation and not to the Bolus operation BZ NOTE Do not use nested loops repeat within a repeat TO INSE
125. r irin nt ene er SEEE EE TE EERE 50 PO PaO PROLHES a E E E A E ETE PU eM PN RP ONUPURUE l OE a Tate PO a A ET E E AAE ET T EET 52 lastre e TON 53 PP OE aa T E E E E T TO 55 Pulse TTOHIS Sassy dressage nn ve UIN IM NIU DERI UU MM D metas 27 Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 3 Table of Contents 4 Poi eonim T T 5U CO cen PE DTOR Prone a rere sea tees senium nears TN D pm TRUE es E bd LER P 60 Gradi ne Poo BU e oneri vente a a aes cea este og E A TIEN ID Un TUR URDU IU EUNT 63 DUO PII DESEE GERE ERREUR EINEN TT A rt errr A A TET Steere NUM UNE 66 Incorporating Advanced Options Commands and I O Into A Method 68 Com ker ieee ee OQ 68 p caterer 69 KEE rio P 70 ee IY TSU TR NTC 71 vio 71 LOT T A a R UD DANHINENUEIIIAIMEUIEIEUINIPHUMNE te 12 FO Mb alee cee ee educ edees ie EEUU MM des 12 DOT dn TERT er etree Xm 74 PAESI oir qui NNMERO EET tmm 75 Alonso LA PRO TONS seasainn iSO eea eea aiaa 76 W AO O A eO satay tate ea E E EAEE SEENE A 76 Ronna VC O o E E E E E A O A tURUE 76 Appendint e MUT duros eR 77 JB cuir MITIS dealers T T tat ae renner enn eee mere ere acre ee eres etree ee 77 PAPU Meko B SDOSEADOCTS DOC oarre reer cre meena eter ent nT rok erator pester t
126. r of doses using the keypad If the Doses is greater than one select the Time Lag button and enter the desired length of time between individual doses If the Units of Time associated with Time Lag are not correct for your application select the Units button to toggle between sec seconds and h m s hours minutes seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon When all parameters on the Set Doses amp Time Lag screen are correct choose the Accept button to return to the Step Definition screen Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 63 GRADIENT PROFILE Available in firmware v1 2 2 and later The Gradient profile is used to combine multiple flow streams from different pumps into a common output stream The flow profiles can be flat of ramped smooth flowing or can execute in a stepped format Gradient Delivery Pump Operation Profile When creating or editing a Gradient profile you will define 4 primary operating parameters The combined Flow Rate e The Target Starting and Ending Percentages for each pump in the system e The Target Time that determines the total length of time over which the Gradient profile will be calculated e The Number of Steps that will be created in the profile Ze NOTE See Appendix E External Connections for instructions on setting up a
127. range is from 00 to 99 This address value is used to identify which pump is to receive a command and which pump is responding The first pump in the chain the one connected to the computer must use address 0 zero Configure each pump with its assigned address and baud rate as described in the Pump Settings section of this Guide NOTE Once communication is established if you manipulate the touchscreen you must reestablish communication lt CR gt X NOTE When daisy chaining using the RJ 11 option all pumps must use the same baud rate Values above 38400 are recommended X NOTE If you are using the optional RJ 11 connections for daisy chaining the connection from the computer must also come through the RJ 11 or RS 232 connectors X NOTE System commands and start stop commands can be executed from method or Quickstart screens For parameter commands it is necessary to run from Quickstart screen X NOTE To maximize communication speed as fast as 50 ms rate changes a prefix commands with the symbol to turn off GUI updates ex irate 100 u m b use the NVRAM off command to turn off writes of rate to memory a Using PHD ULTRA Pump Chain Commands Commands may be abbreviated to the first four letters i e address would be abbreviated addr A space must follow the command if arguments are included If the pump address is nonzero the one or two digit pump address precedes the command without any separator
128. reen as described in the Quick Start Method Variables section of this Guide Quick Start Method Variables INFUSE RATE SELECT WITHDRAW RATE SELECT The Quick Start Infuse Rate and Withdraw Rate Select functions define the rate of pumping while infusing or withdrawing liquids respectively The two functions are controlled identically so we have described the operation here only once When adjusting the Infuse Rate you can set the rate in ml ul nl or pl units of volume and use sec min or hr as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Infuse and Withdraw Rate screens BZ NOTE The following instructions describe setting the Infuse Rate The instructions are BA the same for Withdraw Rate On an Infuse Withdraw combination Method you can set both rates on a single screen by toggling between the two rate options using the Infuse Rate or Withdraw Rate buttons at the top of the screen Infuse Rate nl min Infuse Rate 3 020 zislo 1526 xay ear d ml min Almin Publication 5419 002 Rev J HARYARD APPARATUS 04 28 09 1 08 54 PM Enter your flow rate and units and then press the Enter button green check mark to accept or press the Cancel button red X ta cancel your settings and return ta the previous screen HARVARD APPARATUS 04 28 09 1 09
129. ringe Use this button to advance the Pusher Use this button to advance the Pusher Bar in the forward infuse direction bar in the reverse withdraw direction p gt NOTE Operating the Fast Forward and Fast Reverse will not update the Volume or Time counters in the Method Run screens Basic Method Operations The following operations are common across all Method types VIEWING CURRENT METHOD PARAMETERS As described later in this Guide PHD ULTRA pump operation is controlled by Methods which organize all the commands needed to control the infusion withdrawal process The current settings for the pump are displayed in the selection buttons on the Method Main or Quick Start screens HARVARD APPARATUS 10 27 09 10 03 47 AM Enter your settings to the left and then press the green Run or BD plastic 50 ml 26 594 mm Run Preview button when Quick Start screen ile niin sure to set a showing the Infuse g l Withdraw Method buttons with current parameters Paa gt gt E gt For Preloaded or User Defined Methods with multiple steps the Method Main screen displays the first three steps and the last step in the Method operation when all steps do not fit on the screen In the following sample screen the first three steps Delay Ramp Constant Rate are displayed in one box button and the last step Ramp is displayed beneath them Quick Start Infuse Withdraw HARVARD APPARATUS METHOD Select the desired syrin
130. rn to the Method Selection screen Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 77 Appending A Method Follow these instructions to Append a previously created Method into a new Method 1 From the Quick Start or Method Main screen choose the Method Select button to enter the Method Selection screen 2 From the list of available Methods select press once the Method you want as the base Method 3 Choose the File Options button to enter the File Options screen 4 Choose Append to access the Method to Append screen 5 Choose press twice the Method you would like to Append attach to your base Method The pump will return you to the File Options screen and display a confirmation that the Method was copied 6 Choose Back to return to the Method Selection screen Deleting A Method Follow these instructions to delete a previously saved Method 1 From the Quick Start or Method Main screen choose the Method Select button to enter the Method Selection screen 2 From the list of available Methods select press once the Method you want to delete then choose the File Options button to enter the File Options screen 3 Choose Delete Method The application will display a warning message in the Message Area asking you to confirm your deletion request 4 Choose Delete Method again to confirm the deletion and return to the Method Selection screen Publication 54
131. ronization commands are communicated via Pin 6 e Event signals are communicated via Pin 2 I O TRIGGER OUT The I O Trigger out command is used to send a signal to an external device when the Step in the Method is reached HARVARD APPARATUS a m Select which trigger port the EN signal will be sent aut af NN Advanced Options I O Trigger out Selection Screen Ld The following options are available when setting an I O Trigger out command Valve Auto Trigger 1 Specifies output via the Trigger 1 Pin 4 port When selected you must also specify whether the signal to be sent is High Signal or Low Signal High Signal Sets the output signal to logic high Low Signal Sets the output signal to logic low e Trigger 2 Specifies output via the Trigger 2 Pin 5 port When selected you must also specify whether the signal to be sent is High Signal or Low Signal High Signal Sets the output signal to logic high Low Signal Sets the output signal to logic low e Synchronize Select this to send a signal via Pin 6 to a connected pump to trigger activity When selected you must define whether the desired action is to start an Infuse activity or a Withdraw Refill activity Start Infuse Defines the rising edge signal to trigger an infusion action Start Withdraw Defines the falling edge signal to trigger a withdraw refill action e Valve Select automatic or manual valve control au
132. ry response lt If gt Ramp not set up lt cr gt If prompt Of If xl xxx to xl xxx in seconds lt cr gt If prompt NOTE When using the iramp command rate time remaining on display will not reflect A actual pump rate time remaining during ramping IRATE Sets or displays the infusion rate Quick Start mode only The rate arguement may be replaced by max or min to set the maximum or minimum rate respectively lim may be used to display the range limits Command format irate max min lim rate rate units Query response If xI xxx lt cr gt If prompt Of If xI xxx to xl Xxx cr Ift prompt WRAMP Sets or displays the withdraw rates while ramping Quick Start mode only Command format wramp start rate start units end rate end units ramp time in seconds Query response lt If gt Ramp not set up lt cr gt If prompt Of If xl xxx to xl xxx in seconds lt cr gt It prompt BZ NOTE To clear a ramp use CTTIME command Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA WRATE Sets or displays the withdraw rate Quick Start mode only The rate arguement may be replaced by max or min to set the maximum or minimum rate respectively lim may be used to d
133. s seconds To use the h m s option enter your time value in hours minutes and seconds separating each value with a colon When both Time parameters are set to the correct values choose the Accept button to return to the Step Definition screen On the Set Pulse Volumes screen select the Volume 1 button it should be selected by default as indicated by the active data entry box to the right of the button Enter the desired value using the keypad Repeat for Volume 2 If the Units of Volume associated with Volume 1 or Volume 2 are not correct for your application choose the Units button associated with either parameter to enter the Units selection screen Select the unit of measure button ml uul nl pD applicable to your application then choose the Accept button to return to the Set Pulse Volumes screen When both Volume parameters are set to the correct values choose the Accept button to return to the Step Definition screen Select the of Pulses button to enter the Set Pulses screen On the Set Pulses screen enter the desired value using the keypad then choose the Accept button Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 59 BOLUS PROFILE The Bolus Delivery profile is used to infuse a defined dose of fluid controlled by the total volume and the total time the pump operates Bolus profiles are used for infusion only In a bolus profile you set both
134. s that can help you make better use of the PHD ULTRA pump Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 7 General Information WARRANTY AND REPAIR INFORMATION CAUTION Refer to safety information and setting up the PHD ULTRA before plugging in PHD ULTRA pump Manual Description This manual is designed to provide all operational and program information required to operate and maintain the PHD ULTRA series pumps The functions and features are described in the Technical Specifications section Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or materials This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original consumer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow the exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the t
135. screen keyboard type the name that you want to have displayed The display is limited to 15 characters HEN 05 CHAR MAX E 8 ES HR ER ES E IE HORT a oO FL EA ES Ee ESS ZENEN m e ras space s v 4 To type numbers or symbols select the 123 button to toggle the keyboard to numbers symbols mode To return to letters mode press the ABC button 5 When finished entering the new name choose the Accept button to save your changes and return to the Settings screen NOTE To reset the Logo value back to Harvard Apparatus backspace in the Change A Logo text entry box until the field is blank then choose Accept Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 24 Setting Backlight Level You can adjust the backlighting level on the PHD ULTRA touchscreen to optimize viewing in your specific lighting conditions On the Settings screen the Backlight button displays the current setting on the button HARVARD APPARATUS 04 28 09 11 53 05 AM Select an existing percentage or select set other value and then press the Enter button green check mark to accept or press the Cancel button fred X to Set Backlight Level screen return to the previous screen m 70 30 o 20 TO MODIFY THE BACKLIGHT LEVEL 1 From the Quick Start or Method Main screen choose the Settings button 2 From the Settings menu choose the Backligh
136. send a synchronization signal to a connected pump select Synchronize Then select whether you want to start an infusion action Start Infuse button or a withdraw action Start Withdraw button by selecting the appropriate button Choose the Accept button to save your changes and return to the Method Steps Summary screen po NOTE When using triggers the first step in your method should define the trigger starting level High or Low You may then use the trigger command to change the level as desired for your Method Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 74 I O EVENT IN The I O Event Gn command is used to have the pump pause and wait for a signal from an external device or for a manual user input at the touchscreen These external signals are communicated via Pin 2 on the 15 pin I O Connector port HARVARD APPARATUS Input High to Low Input Low to High Select which type of event the step will wait forand then press the Enter button green check mark to accept or press the Advanced Options menu Cancel button fred X to return I O Event in Selection Screen to the previous screen The following options are available when setting an I O Event Gin command Input High to Low Sets the pump to wait for a logic low input signal falling edge e Input Low to High Sets the pump to wait for a logic high input signal rising edge e User
137. sing glass syringes CAUTION See Setting Force Limits section if using Master Satellite configuration Gang Feature The Gang feature allows multiple syringes to be combined into one common output Multiple syringes combined into a common output 1 From the Configuration screen choose syringe select Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 35 HARVARD APPARATUS Syringe 10 27 09 11 30 44 AM Select an existing syringe HS Norm Ject manufacturer or select custom Glass all types green check mark to accept or press the Cancel button red X Syringe Selection Screen Plasti pak to return to the previous screen showing Gang button Micro Mate Glass Glass all types Stainless Steel 2 Choose the Gang button to enter the Set Syringe Count screen HARVARD APPARATUS Syringe Count 107 27 09 11 31 05 AM Enter a value between 1 and 10 and then press the Enter button green check mark to accept or press the Cancel button red X ta cancel your settings and return Gang count ta the previous screen entry screen 3 Enter the number of syringes being used then press the Accept button HARVARD APPARATUS 10 27 09 11 31 51 AM Enter your settings ta the left Gang 4 syringes and then press the green Run or Custom 10 3 mm Run Preview button when finished Make sure to set a Quick Start screen showing target volume gang
138. sponse If Target volume not set lt cr gt If prompt Of If xl lt cr gt It prompt WVOLUME Displays the withdrawn volume Quick Start mode only Command format wvolume Query response If xl lt cr gt If prompt Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual TIME COMMANDS CITIME Clears the infused time Quick Start mode only Command format citime CTIME Clears both the infused and withdrawn times Quick Start mode only Command format ctime CTTIME Clears the target time Quick Start mode only Command format cttime CWTIME Clears the withdrawn time Quick Start mode only Command format cwtime ITIME Displays the infused time Quick Start mode only Command format itime Query response If seconds lt cr gt It prompt Of Ift 7 cr It prompt Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 94 Using The PHD ULTRA 95 TTIME Sets or displays the target time Quick Start mode only Command format ttime target time Query response If Target time not set lt cr gt If prompt Of If seconds lt cr gt If prompt Of If 7 cr If prompt WTIME Displ
139. steps used lt cr gt lt lf gt steps free lt cr gt lt lf gt total steps lt cr gt lt lf gt lt prompt gt The number of steps displayed is a four digit number right justified and padded with spaces FORCE Sets or displays the infusion force level in percent Valid range is 1 to 100 Command format force 1 100 Query response lt lf gt lt cr gt lt lf gt lt prompt gt FTSWITCH Sets or displays the footswitch setting The footswitch may be used in a toggle on or off mode momentary a press to run mode falling or a release to run mode rising Command format ftswitch mom rise fall Query response If Momentary cr If prompt Of If Active high lt cr gt If prompt Of If Active low lt cr gt If prompt Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA POLL Sets or displays the polling mode state When polling mode is off prompts are displayed when an event happens such as a target being reached When polling mode is on prompts are not displayed when an event happens and a XON character is output when the pump is ready for another command When polling mode is in remote mode the following occurs e Prompts are not displayed e Carraige returns are not displayed e The pump address is displayed even if 0 e cho is forced off and
140. sure that the barrel flange is within the Syringe Holder Block s Retaining Bracket 5 and the plunger flange is within the Pusher Block s Retaining Bracket 4 Close up of syringe positioning with plunger inserted in retaining bracket of pusher block 4 Lower the Syringe Barrel Clamp 12 by screwing down the Syringe Clamp Knob 6 until the clamp is tight against the syringe barrel 5 Push the Retaining Brackets 4 5 tightly against the syringe flanges then tighten the retaining brackets using the Bracket Clamping Knobs 7 8 6 Squeeze the Release Tabs 1 and move the Pusher Block 2 to the right until the syringe plunger is located as far into the syringe as it will travel 7 Using the Allen Wrench located in its holder in the rear of the Pulley Cover 11 loosen the Infusion Mechanical Stop 9 and slide it to the left until it is in contact with the Pusher Block 2 Use the Allen Wrench to tighten it in this position 8 Squeeze the Release Tabs 1 and move the Pusher Block 2 to the left until the plunger is located just to the left of the maximum volume allowable Use the Allen Wrench to loosen the refill Mechanical Stop 10 and slide it to the right until it is in contact with the Pusher Block 2 Tighten the Withdraw Mechanical Stop 10 and then return the Allen Wrench to its holder 11 Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA
141. syringe s and measure syringe bore diameter and enter actual dimensions in millimeters mm using the Custom Syringe entry option Additionally make sure that the guide rods and lead screw are properly lubricated RS 232 Difficulties Verify that the baud rates data framing parameters data bits parity and stop bits on all devices are the same Verify flow control handshaking is set to None If an address is set to other than address make sure commands and queries are prefixed with the address Make sure straight through pinning is used for the cables do not use null modem cables Erase Clear Disc There may be an instance where you want to delete all of your custom methods from the pump There may also be instances where you may need to delete clear the disc in order to power up the pump To do so e Turn the pump on When the start up screen appears press the 4 corners of the display in a clockwise order starting at the top left After each corner is correctly pressed it will turn dark gray e Press yes to confirm or wait 5 seconds to allow the pump to start normally without clearing the disc e There is a 5 second time limit for each press of the screen If the time limit expires the pump will start normally without clearing the disc HARVARD APPARATUS PHD ULTRA Press the 4 corners of tbe start up screen display in a clockwise order to delete clear tbe disc PUMP TYPE INFLUSE WITHDR Avv PROGRAMMABLE Pump fir
142. t TTL Logic Output Default Low Control thru method or serial comm 6 Sync Output TTL Logic Output Rising Edge Start Infuse Falling Edge Start Refill 7 Direction Output TTL Logic Output High Refill Low Infuse 8 Valve Output controls voltage only TTL Logic Output High Valve Actuated Low Valve Off NOTE Valve follows direction change when set to auto 9 13 Signal Return Ground 14 Run Indicator Voltage 5V through a 470 ohm resistor connect to LED Anode 15 Run Indicator Output TTL Logic Output active low dow run connect to LED Cathode Electrical Specifications Inputs Na AV I 20pA V 0 4V I 0 555nA Outputs Von 2 3 8V I y 6mA Vo lt 0 4V I 6mA Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 112 Analog Input Option HARVARD APPARATUS IUIS DNE Welcome ta the Analog contral Screen Press Enter button green check mark to accept or press the Cancel button fred X to return to the previous screen Flow Analog Control Screen Speed 0 Oto 10V Ground A BNC Connector NOTE Voltage must be set to O VDC when pump is turned on NOTE Although the Analog Mode cycle time is 100 ms when changing from min to max or max to min rates please allow a minimum of 250 ms NOTE Not available on satellite units BEBE Publication 5419 002 Rev J Harvard Apparatus P
143. t button to enter the Set Backlight Level screen 3 Select the desired preset level value or use the Set Other Value button to enter a custom level 4 If you select Set Other value enter the desired setting as a percentage value 10 100 then choose Accept to confirm your setting 5 When you have selected or entered the desired backlighting value choose Accept to save your changes and return to the Settings menu Setting Pump Address Value The Pump Address value is used to define a programmable address that can be used in external software applications that will call each PHD ULTRA pump individually TO MODIFY THE PUMP ADDRESS 1 From the Quick Start or Method Main screen choose the Settings button 2 From the Settings menu choose the Pump Address button to enter the Set Pump Address screen 3 Type the desired address value using the onscreen number pad Valid entries are 00 99 4 When you have entered the desired address value choose Accept to save your changes and return to the Settings menu Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 25 Setting the Pump Baud Rate For attaching the PHD ULTRA to an external device using RS 232 or RJ 11 serial communications you can easily adjust the baud rate to optimize communications TO ADJUST THE PUMP BAUD RATE 1 From the Quick Start or Method Main screen choose the Settings button
144. ter button Set Audible Alarms screen green check mark or press the p A Tabl Cancel button red X to return SOLEUS HAC OETA to the previous screen options and with Mute All selected The remaining alarm options are greyed out in this example Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 19 Setting Up The PHD ULTRA 20 TO SET THE USER CONFIGURABLE AUDIBLE ALARMS 1 Inthe Audible Alarms menu select the specific alarm or alarms that you wish to set On the Settings screen select Touch Screen Lock Enable Note that a checkbox on the button will be checked to indicate that you have selected an option but the button will not be displayed in magenta as with other select options in the software 2 If you select the Near End of Run Alarm choose the Percentage Set button to access the Near End of Run Select menu that is used to define at what point in the Method the alarm will sound Select a preset value 75 80 85 9096 95 or choose Set Other Value and Enter the desired Percent Done value using the keypad If you choose Set Other Value choose the Accept button when finished When you have made your selection on the Near End of Run Select screen choose the Accept button to complete your changes HARVARD APPARATUS 04 28 09 11 46 29 AM Select an existing percentage or select set other value and then press the Enter button green Set Audible Alarms Near check mark
145. the time the pump operates and the total volume to be delivered The pump automatically calculates the flow rate based on the syringe selected The flow rate is constant during a Bolus Delivery profile Bolus Delivery Pump Operation Profile Time Volume When creating or editing a Bolus Delivery profile you will define the following operating parameters e The Target Volume to be infused e The Target Time value which is the total time the pump will operate c Step amp 4 Bolus HARVARD APPARATUS 04 28 09 1 28 28 PM Enter the step settings Calculated Rate Rate 100 ml min Step Definition screen for a Set Target Volume 50 ml Bolus Delivery Profile L1 Set Target Time 30 second s Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 60 TO SET UP EDIT A BOLUS DELIVERY PROFILE 1 From the Step Definition screen for a Bolus Delivery profile choose the Set Target Volume button 2 On the Set Target Volumes screen enter the desired value using the keypad 3 Ifthe Units of Measure are not correct for your application choose the Units button to enter the Units selection screen Select the unit of measure button ml uul nl pD applicable for your application then choose the Accept button to return to the Set Target Volumes screen 4 When the Volume parameters are correctly set choose the Accept button to return to the Step Definition screen
146. the echo command is illegal Command format poll on off remote Query response If OFF lt cr gt If prompt Of Itf ON lt cr gt It prompt xon Of REMOTE lt If gt TILT Calibrates the tilt sensor Command format tilt Query response If Calibrating the tilt sensor lt cr gt If Tilt calibration value is lt cr gt If prompt TIME Sets or displays the date and time Command format time mm dd yy hh mm ss Query response lt If gt 4 HHHH HH M lt cr gt If prompt The date order format is dependent on the settings made when setting the date from the touch screen The time format is 24 hours Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual 87 Using The PHD ULTRA 88 SYRMANU Sets or displays the syringe manufacturer Quick Start mode only Valid 3 character manufacturer codes are shown in the table below Air Tite HSW Norm Ject Typing syrm with no arguments displays the Pap Becton Dickinson Pusbpdk Typing syrm displays a list of manufacturers with their associated 3 letter code Typing syrm code shows a list of syringe sizes associated with the specified nada ir Command format syrm 3 char code volume ul ml Wejp If manufacturer diameter mm lt cr gt Query response It
147. the pump address Valid range is 0 to 99 Command format address 0 99 Query response lt lf gt Pump address is lt cr gt If prompt Notes Pumps with an address of 0 are masters and pumps with an address between 1 and 99 are slaves BAUD Sets or displays the RS232 port baud rate Valid baud rates are 9600 19200 38400 57600 115200 128000 230400 256000 460800 and 921600 Note that some computers may not be able to handle baud rates above 115200 Command format baud 9600 19200 38400 57600 115200 128000 230400 256000 460800 921600 Query response If baud cr If prompt Notes If this command is entered via RS232 the prompt is displayed at the previous baud rate and then the baud rate is changed CATALOG Displays a catalog of methods stored in the pump Command format cat Query response If 2 Method name Size lt cr gt lt lf gt _ __ __ __ _ lt cr gt If method name lt cr gt Thisline is repeated for each file on the disk It 1t file s using steps lt cr gt It prompt Notes The file size is the number of steps in the method plus one for the file information header The last line shows the number of files and the total number of steps being used on the disk Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Usin
148. the user defines the total number of repetitions pulses for the oscillating pattern Each segment of the profile must be set to run for either a specific length of time or to deliver a set volume Pulses Pulse Flow Pump Operation Profile Time Volume When creating or editing a Pulse Flow profile you will define four primary operating parameters e The Flow Mode defining the direction of flow infuse or withdraw e The Flow Rate to be used at each level of the pulsed profile within the pumping cycle e The length of Time that each segment of the profile will run or the specific Volume to be infused withdrawn during each segment of the profile The Number of Pulses repetitions that will be executed during the pumping cycle During a Pulse Flow profile the PHD ULTRA calculates a series of equal length pumping stages at two user defined flow rates a starting rate and a pulse rate You enter the Number of Pulses repetitions that you want to use along with how you want the system to calculate the length of each stage by time or by volume pumped A Pulse Rate profile can be applied to pulses that are higher in rate or lower in rate than the starting flow rate When creating or editing a Pulse Flow profile you can set the rate in ml ul nl or pl units of volume using sec min or hr as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined
149. tified for country of use Plug the power cord into the Universal Power Input connector on the rear of the PHD ULTRA 2 Turn on main power switch located in the upper left corner of the rear panel The PHD ULTRA touchscreen display will illuminate and display the startup screen while performing initial self diagnostics When complete the unit will display either the Quick Start screen or the Method Main screen REMOTE PUMP PHYSICAL CONNECTIONS RS 485 Remote System HARVARD APPARATUS RS 485 OUT 4 4 RS 485 IN To connect an RS 485 remote system 1 Onthe control box connect the RS 485 cable to RS 485 OUT connector 2 Onthe remote unit connect the RS 485 cable to RS 485 IN connector Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 15 NAVIGATING THE PHD ULTRA EZ PRO SOFTWARE The PHD ULTRA uses a high resolution touchscreen with an icon enabled graphical user interface and an advanced methods architecture to streamline simple to complex tasks The PHD ULTRA Workspace The PHD ULTRA touchscreen interface is divided into three basic areas e e Operations Display this section of the screen is used to display K pump operations method information general parameter settings and the real time display of data while the pump is running e o o Message Area this section is used to display the pump name the time dat
150. tomatic is controlled by pumping pin 8 Valve On Activates the valve Walve Off De Activates the valve Valve Auto Returns the pump to Automatic valve control Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 73 TO INSERT AN I O TRIGGER OUT COMMAND 1 2 3 4 5 6 From the Method Main screen choose Step Definition to enter the Method Steps Summary screen Highlight Select once the Method Step that you want the I O Trigger Out to follow Choose the Add Step button to access the Method Steps Menu screen Choose Advanced Options to enter the Select Advanced Options screen Choose the I O Trigger out button to enter the Trigger Step Definition Screen Set your Trigger settings by choosing the appropriate option from the following sub procedures a To send a Trigger signal to an external device via Pin 4 select the Trigger 1 button To send a Trigger signal via Pin 5 select the Trigger 2 button Then select whether the signal will be High or Low by selecting the appropriate button b To send a trigger signal to an external valve select Valve Then select whether the trigger will actuate On button or close Off button by selecting the appropriate button This overrides the Valve Auto setting which means that the valve is controlled by a direction change The valve will default back to auto after leaving the Method Run screen c To
151. ton to toggle between Month Day Year m d y Day Month Year d m y and Year Month Day y m d formats Enter your values in two digit format for each value For example April 3 2009 would be entered as 04 03 09 in m d y format 3 To enter the time select the Time button and type the desired time using the onscreen number pad Select the Time Period button to toggle between AM PM and 24 hours settings Time should be entered using hours minutes seconds hh mm ss Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 47 4 When the desired values are entered choose the Accept button to save your Changes and return to the Method Main screen When a Time Delay value is active the Time Delay Parameters button is displayed in Red on the screen to alert the user that a delay has been set 5 Press the Run button to activate the Delay HARVARD APPARATUS change Current settings 16 55 09 11 52 21 AM Infuse Withdraw i plastic 26 59 mm 50 00 ml Pump will run at the following Target a0 mi date and time 10 28 08 Infuse 5 ml min 12 00 00 PM Press Withdraw 5 ml min tn here or stop button to cancel delay Flow EH gt Quick Start method with a Direction PRESS TO CLEAR MESSAGE Time Delay set le LE 0 000 ml Time Elapsed 00 00 00 Time Remaining 00 20 00 Total Inf Volume O ml Force Level 100 CREATING AN EZ PRO NEW METHOD If none of the Met
152. tons displayed one for each satellite HARVARD APPARATUS METHOD 1 Select the desired syringe and define steps Method Main screen showing an active remote with an address of OI Stepped amp Constant Rate Sherwood 140 ml 37 95 mm HARVARD APPARATUS 1 steps Force Level 100 03 30 10 02 22 52 PM Method Main screen BD plastic 60 ml 26 59 mm Ready to run method sbowing a Gradient system witb 2 active satellites addressed Gradient b 02 Ol and 02 Pump ll i O1 is being controlled Fy i by the master Total Yolume Dispensed 0 ml lt q 4 D Dnm The pump control button s background color indicates the status of the remote unit Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices 119 e Blue Background Connected Motor Stopped e Red Background Comm Error e Yellow Background Motor Stall e Green Background Motor Running The user can select which pump to control by pressing the button with its corresponding address The currently selected pump s button will contain an interior white box When a satellite pump is being controlled the Fast Forward Fast Reverse buttons on the master will move the satellite s pusher block HARVARD APPARATUS 03 30 10 02 13 48 PM showing a comm error with the remote pump addressed O1 Sherwood 140 ml 37 95 mm Stepped amp Constant Rate Setting Addresses on Remote Sat
153. ttings f WARNING When using low volume small diameter syringes or pumping menu to avoid accidental damage to the syringe p NOTE For Master Satellite configurations changing diameter at the Master will BA change the diameter at all satellites TO SELECT A SYRINGE USING THE SYRINGE LOOKUP FUNCTION 1 From the Quick Start or Method Main screen choose the Syringe Select button HSW Norm Ject Glass all types Plasti pak Micro Mate Glass Glass all types Stainless Steel HARVARD APPARATUS 10 27 09 11 30 44 AM Select an existing syringe manufacturer or select custom syringe and then press Enter green check mark to accept or press the Cancel button fred X to return to the previous screen Syringe Selection screen with currently selected syringe 2 From the Syringe Selection menu double tap the Manufacturer Model button for the desired syringe to enter the Syringe Size Selection screen Harvard Apparatus Stainless Steel 4 851 mm 2 5 ml 9 525 mm 8 ml 19 13 mm 70 ml 28 6 mm 50 ml 34 9 mm 100 ml HARYARD APPARATUS 04 28 09 12 22 56 PM Select the desired syringe size and then press Enter button green check mark to accept or press the Cancel button red X ta return to the previous screen Syringe Size Selection screen showing barrel dimensions and syringe capacity 3 Double tap the dimensions capacity button for the syringe you will be using Publication 5419 002 Rev J Harvard App
154. tton when finished Make sure to set a target volume Infuse Withdraw Quick Start Method screen Quick Start Infuse Withdraw 1 From the Quick Start screen the Infuse Withdraw Quick Start Method should be displayed in the Method Select button If that Method is not displayed select the correct Method using the procedure outlined in Running The Pump 2 Choose the Syringe Select button to enter the Syringe Selection screen 3 Follow the instructions in the Syringe Selection section of this Guide to complete the selection of a syringe from the built in Syringe Lookup Table or to create a new syringe When finished choose the Accept button to save your changes or choose the Cancel button to cancel your changes and return to the Quick Start screen Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 41 4 Choose the Infuse Rate Select button to access the Set Infuse and Withdraw Rates screen Enter the required parameters on the Set Infuse and Withdraw Rates screen Details on setting the Infuse and Withdraw Rates are provided in the Quick Start Method Variables section of this Guide When finished choose the Accept button to save your changes or choose the Cancel button to cancel your changes and return to the Quick Start screen Choose the Target Volume Select button to enter the Set Target Volume screen Enter the required data on the Set Target Volume sc
155. tus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA 16 The PHD ULTRA supports touchscreen orientation auto rotation landscape portrait for permitting easy use of the pump when positioned upright on its side This configuration and the associated touchscreen display are shown in the following figure Quick Start Infuse Only Sherwood 38 4 mm 140 ml 220 975 ml min 140 mi HARVARD APPARATUS 05 05 09 4 23 55 PM Enter your settings to the left and press the RUN or Run Preview button Make sure to select the appropriate syringe HARVARD APPARAT The PHD ULTRA pump shown in its vertical upright orientation and the associated Quick Start menu screen showing the application auto rotated into portrait mode SELECTING AN ITEM FROM A MENU LIST Some sections of the PHD ULTRA software display a list of choices from which you select These menus require that you first select the item that you want When you do this the item you have selected is highlighted in magenta on the screen To confirm your selection you must choose the Accept button or touch the item a second time An example of the two step selection process with a button color displayed in purple is shown below HARVARD APPARATUS 10 27 09 11 37 51 PM Select an existing method or create a new method and then press the Enter button green Tbe EZ PRO Method check mark CHO Selection screen sbowing a button
156. tus PHD ULTRA Syringe Pump Series User s Manual Using The PHD ULTRA 61 During a Concentration profile the PHD ULTRA operates at a constant infusion rate and automatically stops when the target dosage rate is reached When creating or editing a Concentration flow profile you can set the rate in ml ul nl or pl units of volume using sec min or hr as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set Rate and Concentration screen The ingredient concentration is entered in mg ml HARVARD APPARATUS 04 28 09 1 29 16 PM D grams Enter your information and then press the Enter button green check mark ta accept ar press cancel your settings and return to 0 ima ml Set Dose or Duration the previous screen a Concentration Delivery 0 ima kg D secondis profile 0 Dosets 0 secondis TO SET UP EDIT A CONCENTRATION DELIVERY PROFILE 1 From the Step Definition screen for a Concentration profile choose the Animal Weight button to enter the Set Animal Weight screen HARVARD APPARATUS Weight grams 04 28 O9 1 wo po PM Enter the animal weight and then press the Enter button green check mark ta accept or press the Cancel button red X to cancel your settings and return to Set Animal weight screen in the previous screen Concentration profile
157. ual 54 Using The PHD ULTRA 55 STEPPED PROFILE The Stepped Rate profile is used to create an increasing or decreasing flow rate that varies in a series of equal steps between a starting and ending flow rate Stepped Rate Pump Operation Profile Time Volume When creating or editing a Stepped Rate profile you will define four primary operating parameters e The Flow Mode defining the direction of flow infuse or withdraw e The Flow Rate at the beginning and end of the pumping cycle e The Target Time that determines the total length of time over which the stepped profile will be calculated e The Number of Steps that will be created in the profile During a Stepped Rate profile the PHD ULTRA calculates a series of equal rate increases that will vary the flow from the starting value to the ending value You enter the Number of Steps you want to use The Target Time defines the total length of pumping time with the Target Time value divided equally into each stepped rate A Stepped Rate profile can be applied to an increasing or decreasing rate application When creating or editing a Stepped Rate profile you can set the rate in ml ul nl or pl units of volume using sec or h m s hours minutes seconds as the units of time The system automatically calculates a minimum and maximum flow rate based on the syringe selected or custom defined The min max values are made available as button selection options on the Set screen
158. us screen Baud Rate The Settings screen 115200 Address O Power Up Setting the Audible Alarms The PHD ULTRA pump incorporates an Audible Alarm feature that can be used to warn the operator about various operating conditions errors and power outages The following alarms are user configurable in the Settings section of the application e End of Run An alarm will sound when a Method run is completed Touch anywhere on touchscreen to silence the alarm e Power Fail An alarm will sound if power to the PHD ULTRA is lost while the pump is running Pressing any button on the screen silences this alarm e Motor Stall An alarm will sound to notify the operator of any motor stall e Keypad Clicks A soft beep indicates a positive touch on any button on the screen e Near End of Run This alarm 3 beeps can be custom configured to notify the operator that a Method is near completion The user can select from preset values of 75 80 85 90 or 95 completion or can set a custom value using the touchscreen e Calibration Reminder This alarm is used to inform the user that the system has reached its scheduled Calibration date It is checked at each power up e Mute All Selecting this option mutes all audible alarms When selected the Mute All button is indicated with a checkmark and the remaining buttons on the screen are greyed out HARVARD APPARATUS 04 28 09 11 45 30 AM Select the desired alarmis and then press the En
159. using the Bracket Clamping Knob 4 BZ NOTE Mechanical stops are set using the same steps as a standard 2 syringe holder Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Appendices Microliter Multi Rack Configuration Thumb Nut Syringe Holder Adjustable Syringe Holder Syringe Clamp Bracket Clamping Knobs Syringe Holder Bracket Clamping Knob Pusher Block PHD ULTRA Retaining Bracket Syringe Holder Retaining Bracket Pusher Block Thumb Nut HARVARD Syringe Clamp APPARATUS PHD ULTRA showing Pusb Pull Mechanism installed To load syringes on the push pull pump 1 Loosen Thumb Nut 1 to release the Adjustable Syringe Holder 2 2 Loosen the Retaining Bracket 7 on the Pusher Block using the Bracket Clamping Knob 5 3 Loosen the Retaining Bracket 6 on the Adjustable Syringe Holder 2 note that there are bracket clamping knobs 4 on both the front and rear of this assembly that need to be loosened 4 Extend Plunger on syringe s to maximum capacity Loosen Thumb Nut 8 and lift Syringe Clamp 3 Place the syringe on the Adjustable Syringe Holder 2 extend as necessary then lock into place by tightening the Thumb Nut 1 Lock the syringe flanges into place by tightening the Retaining Brackets 6 7 Secure the syringe clamp 3 and tighten using the Thumb Nut 8 Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syrin
160. w 20 80 RH non condensing Continuous Class 1 lI Harvard Apparatus 84 October Hill Road Holliston MA 01746 CE ETL UL CSA WEEE EU RoHS amp CB Scheme ANSI UL 61010 1 Ed 3 CAN CSA C22 2 No 61010 1 Ed 3 IEC 61010 1 Ed 3 CENELEC EN 61010 1 CB Scheme IEC 61326 1 Ed 1 FCC 47CFR 15B Class A Fan option is required if external operating ambient is expected to be gt 35 C Relative Humidity 100 ari all fu BU a0 Al al 20 10 IN Mote As temperature increases maximum allowable humidity decreases F Operating Humidity vs Temperature 10 15 20 29 al dg 40 Ambient Temperature C NOTE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Setting Up The PHD ULTRA
161. wo year warranty period promptly contact Harvard Apparatus 84 October Hill Road Holliston Massachusetts 01746 using our toll free number 1 800 272 2775 or outside the U S call 508 893 8999 Email Address is bioscience harvardapparatus com Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges for non warranty repairs Please allow a reasonable period of time for completion of repairs or replacement If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using a part number obtained from our Technical Support department If practical enclose a sample part or sketch We offer a complete reconditioning service Serial Numbers All inquiries concerning our product should refer to the serial number of the unit located on the rear panel Calibrations All electrical apparatus are calibrated at rated voltage and frequency While the flow and volume will stay calibrated the peak pressure may vary Harvard Apparatus recommends an annual cali
162. xt entry box 4 On the Method Name screen type the new name for your Method up to 15 characters using the onscreen keyboard Choose the Symbols Numbers button 123 to display the list of available numbers and symbols that can be included in the Method Name To switch the keyboard back to letters mode choose the Letters button ABC 5 When finished entering the new Method Name choose Accept to save your changes and return to the File Options screen 6 Choose Back to return to the Method Selection screen Renaming A Method Follow these instructions to rename a previously saved Method 1 From the Quick Start or Method Main screen choose the Method Select button to enter the Method Selection screen 2 From the list of available Methods select press once the Method you want to rename then choose the File Options button to enter the File Options screen 3 Choose Rename Method to access the Method Name Entry screen The current Method Name will be displayed in the text entry box 4 On the Method Name screen type the new name for your Method using the onscreen keyboard Choose the Symbols Numbers button 123 to display the list of available numbers and symbols that can be included in the Method Name To switch the keyboard back to letters mode choose the Letters button ABC 5 When finished entering the new Method Name choose Accept to save your changes and return to the File Options screen 6 Choose Back to retu
163. y When all parameters are correctly set on the Set Pulse Rates screen choose Accept to confirm your settings and return to Step Definition screen Select the Volume or Time button to determine how the pump cycle length will be managed Volume calculates each cycle stage by pumping for the length of time needed to pump the specified volume Time manages each cycle stage by pumping for the amount of time specified Depending on whether you have selected the Volume or Time button in the preceding step choose the Set Time or the Set Volume button to enter the Set Pulse Times or the Set Pulse Volumes screens HARVARD APPARATUS 04 28 09 1 27 52 PM Enter your times and then press the Enter button green check mark ta accept or press the Cancel button red X to cancel Set Pulse Times screen used your settings and return to the to define the length of time previous screen the pump will operate at each pulse step level If you selected Set Time continue with Step 11 If you selected Set Volume proceed to Step 14 On the Set Pulse Times screen select the Time 1 button it should be selected by default as indicated by the active data entry box to the right of the button Enter the desired value using the keypad Repeat for Time 2 If the Units of Time associated with Time 1 or Time 2 are not correct for your application select the Units button associated with either parameter to toggle between sec seconds and h m s hours minute
164. y ere 78 pcs a isst Boro NC T m 80 eo Aeaee cial Boia oct er NH TS 80 Pan e a T E AAE E AET ere E 0 T 82 Usinas twe PHD ULTRA Pump Chain COmMmMmaNd Sssssiersesrerassirisieorsessstietieiisreiieisiite 82 Pee TG e acetate A A EE ENE EEN E 83 eO n PICS anne NNT A rere nn cen a A E ee eee ne ere 84 Ron N eE ent ses sets UBI vp ceso eee on E E E NU UN rcr NNI UID 89 Ite COTAN ea N A EMEN UDIM TM MIN 90 vorme COPPmaid ea dI etn N 92 pne O a era E E E E A A PELA AM P E OE N N AN 94 By OC O ea E E ee E 95 Bie RD aa oT Ea A A E AE OAA S EEA AE 96 Publication 5419 002 Rev J Harvard Apparatus PHD ULTRA Syringe Pump Series User s Manual Table of Contents 5 jA 0 0121 Tal Te ee E 97 Appendix A Sytinge V olume Diaineter Reference T4blenca oerte titi eettasas II Appendix B Stainless Steel Sy M0 iuc P 98 Appendix C Mia Max Flow Rates siisii em ne en nn mene tton db epit ene 99 APPa Doni c PEI9Idet6 DDIOFIS o aisi VMe teo Um cotes PUn eer eT en Inc M UE UI EE term 100 Mountne eh A A IN ere C 100 Mountung a67 TO Muli Rac cee eee rere erent ner ndun ee ere Seo eer ee 102 Syringe Loading 4 x 140 amp 6 10 Multi Racks eno nemine dotate tet iate tiu 104 Mont a Miciolt i Mali RICK essei esmesseooribeye fendposa asninn nO SR T PUENTES EE EEVEE E 106 Syringe Loading Microliter IDE DOC eerte duledudedonntedatueb ueni e pitiot qunfgu eddie dde dnd ieget 108 oringe
165. you to the Method Selection screen on the touchscreen When you are likely to use the touchscreen to enter alphanumeric data e g a Method name or a parameter numerical value through a simulated keyboard or number pad the specific key is mentioned or we indicate you should enter your desired values e Select The term select refers to highlighting an item or moving the button focus on the touchscreen Selecting an item prepares it for an action and requires a second touch on the touchscreen to confirm the selection For example when you select the Syringe Manufacturer Brand on the Syringe Selection screen it is first highlighted in magenta Pressing the selected choice a second time confirms accepts the selection You can also press the Accept button to confirm the selection We will use the term select to refer to this double press or press confirm action throughout this guide Illustrations Unless otherwise indicated the values in the illustrations of this manual are examples only They are not intended to indicate the exact values you will see or to suggest the values you should use Special Messages Callouts The following special messages and callouts appear throughout the guide to indicate information that requires special attention Warnings or hazard instructions provide information to help you avoid personal injury or damage to the PHD ULTRA Pump during operation LE Notes provide helpful instruction

NOTE - Hugo Sachs Elektronik

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