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Leica Microsystems - Microscopen Specialist

1. Optical data of Leice 1x Plan 1x Planapo 1 6x Planape 0 63 Planapo 0 5 Plan 0 32 Achromat 05 gt Achromat 1 5 Achromat Objectives 1x Achromat 2x Achromat 0 8 Achromat f 0 53 Achromat 0 8x Plan 10x 21B Ergo objective Objectives 0 40 53 Working distance mm 153 5 mm Eyepieces Working distance mm 97 Planapo 297 Achromat 187 Achromat 112 Achromat Magnification changer position Magnification changer position Total magnificatian l ield diameter mm Total magnification Field diameter mm Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm MS5 Positions 0 63 1 1 6 2 5 4 When using the planachromatic and planapochromatic objectives MZ125 the magnification is increased by the factor 1 25x A un Lo 2 t1 Gn 1 Co wom on 62 Leica M Stereomicroscopes User Manual Leica M Stereomicroscopes User Manual Optical data of Leica MZ75 MZ95 1 63x Planapo 0 5x Plan 0 32x Achromat 0 5x Achromat 1 5x Achromat Objectives 8x Achromat 0 63x Achromat Working distance mm 81 Plan 187 Achromat 49 Achromat 1 6x Planapo 2x Achromat
2. Attachment for vertical ohlique observation 10 446 393 10 446 393 C Compatible CA Compatible if used with intermediate ring order separately 0 Intermediate ring recommended M Magnification increased by factor 1 25x Remove intermediate ring 10 446 393 already included with MZ95 33 Microscope carrier gt drive housing Microscope carrier AX 34 Optics carrier 3 microscope carrier Apply the adhesive scale of magnifications Microscope carrier The microscope carrier can be attached to the focusing drive in 2 positions For short working distances and for flat objects use Position 1 Use this position also in conjunction with the attachment for vertical and oblique observation see detailed in structions Use Position 2 for long working distances and for bulky objects gt Use an Allen screw to secure the micro scope carrier in the position required A connection socket for a 4 mm grounding cable is provided on the microscope carrier Different models are available for MS5 MZ6 MZ75 MZ9 and for MZ12s MZ16 and MZ16 A see p 18 he microscope carrier AX can only be fitted in position 1 to the focusing drive Optics carrier gt Gently lower the optics carrier into the microscope carrier gt Use the screw to secure the optics carrier in the position required Only for MZ7s gt Remove the screws gt Remove the rotary knobs
3. T NN SS Coaxial illuminator This illuminator is for observing and photographing flat highly reflecting objects such as wafers integrated circuits and polished metal sections The coaxial incident light housing fits like an accessory tube between the optics carrier and the binocular tube or between the optics carrier and the phototube page 50 For the MZ7s and MZ9 fit the objective oreferably to the spacer ring 10 446 300 page 32 This will completely illuminate the largest field of view e A quarter wave plate is required when the microscope carrier AX is used in the vertical position gt Using the clamping screw secure the quarter wave plate to the objective mount gt Using the knurled ring turn the quarter wave plate until the required effect is obtained Please observe the notes on the use of the aperture in the AX microscope carrier p 19 Leica M Stereomicroscopes Illuminations 47 48 For MS5 MZ5 For Leica MZ7s MZ9 MZ12 Near vertical illuminator The near vertical approx 5 light beam illuminates depres sions and cavities Shadowing by tools is eliminated This illuminator can only be used with the achromatic objec tives having focal lengths of 100 150 175 and 200 mm Spacer rings are required for the Leica MZ75 MZ35 MZ125 to secure the near vertical illuminator to the optics carrier page 32 gt Unscrew the objective gt Release the c
4. e For zooming with MZ16 A see the user manual M2 116 0 MS5 5 steps MZ6 6 1 zoom MZ7s 7 9 1 zoom MZ9s 9 5 1 zoom MZ12s 12 5 1 zoom MZ16 and MZ16 A 16 1 zoom e The magnification changer can be operated from either the right or the left side gt Look into the eyepieces gt Focus on the object page 16 gt Turn the magnification changer to the desired position emm See MZ6 0 8 1 1 25 1 6 2 2 5 3 2 MZ7s 0 8 1 1 25 1 6 2 2 5 3 2 4 MZ9s 0 8 1 1 25 1 6 2 2 5 3 2 4 5 MZ12s 1 1 25 1 6 2 2 5 3 2 4 5 6 3 8 MZ16 0 8 1 1 25 1 6 2 2 5 3 2 4 5 6 3 8 10 Engage the ratchets whenever magnifications need to be exactly reproducible gt For MZ6 MZ9s MZ12s and nidi MZ16 Press the switch gt For MZ7 Turn the knurled ring counterclockwise For setting ratchets at the MZ16 A see the user manual M2 116 0 Leica M Stereomicroscopes peration Scale of magnifications Magnifications and fields of view Magnification with MZ12 MZ16 planachromatic and planapochromatic objectives MS5 MZ6 Display of magnification factors from 0 63 4 MZ75s Display of magnification factors from 0 63 5 Corresponding scales for other objective and eyepiece combi nations can be affixed to the rotary knobs page 34 MZ9 Display of magnification factors from 0 63 6 MZ12 Display of magnification factors from 0 8 10 MZ16 MZ
5. Release the clamping screw gt Displace the horizontal arm gt Retighten the clamping screw Leica M Stereomicrascopes Assembly 29 Table clamp stand Swinging arm stand Safety ring column Safety ring gt column 30 Limitation AN Drive housing gt horizontal arm Large swinging arm and table clamp stands gt Use the clamp to secure the column to a table top 20 mm 50 mm thick gt Insert 4 Allen screws into the base from x E e beneath and secure them to the end of DL Na the column The safety ring secures the instrument and must always be positioned beneath the horizontal arm gt Position the safety ring with the screw head uppermost and towards you and slide it down the column gt Retighten the clamping screw gt Lower the horizontal arm onto the safety ring with its end pointing towards you gt Retighten the clamping screw The screw head must engage the notch It limits the lateral movement It may not be removed and neither may the safety ring be milled down If the horizontal arm is swung out beyond the limit the instrument may fall over This section describes the assembly of the focusing drive with side faced carrier rod To fit the focusing drive with peg refer to page 28 IN gt Release the clamping lever gt Tilt the carrier rod downwards gt Retighten the clamping lever gt Slide the drive housing with side faced carrier rod onto the ho
6. gt Set the double iris diaphragm to OPEN gt Adjust the eyecups gt Set both eyepieces to zero gt Set the microscope carrier AX for stereoscopic observation 1 Place a flat test object under the objective 2 Engage the lowest magnification position gt Use the coarse focusing drive to set the working distance page 16 3 Look into the eyepieces P Use the focusing drive to bring the test object into focus Leica M Stereomicrosccpes peration C Adjusting dioptric settings D Inspecting parfocality 4 Engage the highest magnification position 5 Optimize the focus using the focusing drive 6 Engage the lowest magnification position 7 Do not look into the eyepieces gt Rotate the eyepieces counterclockwise in the direction as far as the stop 5 diopters 8 Look into the eyepieces gt Slowly rotate each eyepiece individually in the direction until each eye sees the object sharply imaged 9 Engage the highest magnification position 10 Refocus if necessary 11 Slowly move the magnification changer from the lowest magnification to the highest e The image sharpness must remain constant parfocal Otherwise repeat the procedure Leica M Stereomicroscapes Operation 21 Assembly The components la 1b 2a 2b 3 co N om c Pe Incident light base with stage plate Transmitted light base with glass stage plate Side faced col
7. 10 447 078 is required For a detailed information refer to the user manual Leica CLS The clamp of the fiber optic light guide cannot be attached to the microscope carrier AX gt Turn the clamping screw out of the clamp gt Hold the clamp in the opening of the microscope carrier gt Push the clamping screw through the holes in the microscope carrier and in the broader part of the clamp and screw it finger tight to the narrow part of the clamp gt Insert the fiber optic light guide into the clamp gt Switch on the power unit gt Align the light spot with the object gt Retighten the clamping screw Leica LED illumination The Leica LED1000 Laser Emitting Diode illumination is avail able with ring illuminator and or spot and suitable for routine tasks with the Leica MS5 MZ6 MZ7 MZ9s stereomicroscopes LEDs which do not develop any heat are used as illuminators The color temperature measures 5000 K daylight For details see the separate instructions for use for the LED illumination Leica M Stereomicroscopes Illuminations Supply line lamp 25W Follow the safety instructions given on page 7 SAN AW g NN gt Fit the lamp holder and lamp as described below incident light lamp 6 V 10 W gt Connect the 25 W supply line lamp to the power supply gt Switch on the supply line lamp gt Align the light spot with the object as described Aran EM
8. 445 157 0 5x Plan E 10 447 075 0 8x Plan 10 447 157 1x Planapo d amp 8 10 447 101 2x Planapo lS alsla S 10 472 650 1 6x Planapo Zigis sis s s 3 10 448 236 0 63x Planapo E E E E S Plan Planapo 10 367 929 E E E E E E 8 i a ajajaj ao e 10315 308 10 445 352 es 10 367 929 Leica M Stereomicroscopes Assembly CA 10446 172 M_ CA 10446 172 M CA 10446172 M 10 445 819 wo n MI aS TE N 2 x x i El si amp G 0 x eo e E a CA 10 446 172 M CA 10 446 172 M CA 10 446 172 M CA 10446 172 M CA 10 446 172 M CA 10446172M CA 10 446 172 M CA 10 446 172 M CA 10446 172 M CA 10 446 172 M CA 10 446 172 M CA 10 446 172 M CA 10 446 172 M CA 10 446 172 M CA 10 446 172 M CA 10 446 172 M 10 447 075 10 472 650 10 446 236 10 447 101 lauro oon dou c oiuudep a ae 10 446 157 10 445 618 MI 104610 C c 41 oOpf 04430 010446 300 anapo 1x MZ125 MZ16 anapo 1 6 M2Z12s MZ16 anapo 0 63x MZ125 MZ16 anano 2x MZ125 M716 Coaxial incident lightt MS5 MZ6 MZ 5 MZ95 Microscope carrier AX Microscope carrier AX F P P P Leica M Stereomicroscopes User Manual 10 447 062 MZ12s MZ16 MZ16 A CA 10 446 300 CA 10 446 300 CA 2x 10 446 300 10 445 198 Near vertical illuminator 10 446 393 CA 10 446 300 CA 10 446 300 CA 10 446 300 CA 2x 10 446 300 10 445 156
9. carrier and must manually be turned as follows Please note In the position for stereoscopic observa tion through both beam paths turn the aperture counterclockwise until it stops Otherwise the field of view is underex posed vignetting Inthe position for axial observation through the right or left beam path turn the aperture clockwise until it stops Leica M Stereomicroscopes Operation 19 20 Tips for your work A Preparations B Focus on the test object Adjusting diopter settings and parfocality Leica stereomicroscopes are parfocally matched This means that once you have focused on a feature in the manner ce scribed taking account of diopter settings you can zoom the magnification up and down without refocusing You only need to refocus if you want to look at higher or lower planes in the object A prerequisite is the correct setting of the diopters and the parfoc ality The dioptric range is from 5 to 5 The following adjustments must be carried out only once by each user The procedures for adjusting the dioptric setting and the parfocality are somewhat different if an eyepiece contains a graticule Refer to the user manuals for the MPS modular photomicrographic systems or for measuring gt Install the lamp gt Set the interpupillary distance and if necessary the viewing height gt On the video phototube Move the lever to position VIS and open the diaphragm
10. gt Apply the adhesive scales gt Replace the rotary knobs Leica M Stereomicroscopes Assemoly Eyepiece Ohjective AN Binocular tube gt Release the clamping screw f you have an accessory such as an ergo module page 14 a coaxial illumi nator page 47 or a video phototube page 52 fit it now to the optics carrier Tighten the clamping screw gt Tilt the binocular tube and insert the edge of its dovetail ring beneath the two lugs on the optics carrier gt Move the binocular tube from side to side until the locating screw on the optics carrier engages the quide gt Retighten the clamping screw Optical accessories gt Insert a graticule if necessary page 56 gt Release the clamping screws on the eye pieces gt Insert the eyepieces fully into the eye piece tubes Check that they have seated correctly gt Tighten the clamping screw You can extend the overall magnification range by using 10x 16x 25x and 40x wide eyepieces for persons wearing glasses Hold the objectives during assembly and disassembly to pre vent them from falling on the stage plate This applies particu larly to the 2x planapochromatic objective which is very heavy Remove the specimens from the stage plate gt Screw on the selected objective counter clockwise e Some instrument configurations require spacer rings in order to secure the objectives page 32 These rings are fitted and rem
11. infections if several users are working at the same instrument see p 7 Protect your glasses from being scratched The 10x 21 wide field eyepieces are supplied with laterally tapered soft eyecups Setting the working distance focusing obtaining a sharp image To focus use the focusing drive to raise or lower the stere omicroscope until the feature of interest is in focus working distance to the objective The working distances of the various objectives are listed in the tables on pages 62 66 The focusing drive can be operated from either the right or the left side When focusing on a new object always start with the lowest magnification because the larger field of view makes the object easier to find Afterwards select the desired magnifi cation Leica M Stereomicroscapes Operation Coarse fine drive Adjusting the free inning of the focusing drive Focusing drive MS5 MZ9 Focusing drive coarse fine MS5 MZ12 Focusing drive coarse fine MZ16 MZ16 A gt Place the object beneath the objective gt Engage the lowest magnification page 12 gt Look into the eyepieces gt Using the rotary knob bring the object into focus gt Focus approximately with the larger knob gt Fine focus with the smaller concentric knob Is the focus movement too loose or too tight Does the outfit tend to slide downwards You can adjust the movement in accordance with the we
12. its rubberized upper surface with needles e The movable holder accepts petri dishes gt Place the 120 mm support ring in the 2 120 mm port gt Place the cup stage on it The Leica MATS thermocontrol system is a heating system for microscopes and stereomicroscopes Leica MATS allows the observation of temperature sensitive specimens and living cells in biology medicine and pharmaceutics under exact tempera ture conditions For details see M2 227 0 instructions for use Polarization transmitted light The analyzer in combination with either the rotatable polariza tion stage or the polarizer on glass stage plate enables double refracting materials to be studied These can include organic crystals mineral grains thin sections of rock or bone polymers stressed glass and liquid crystals A detailed description can be found in the user manual M2 277 Leica M Stereamicroscopes Accessories 57 The field of view is partly shadowed The image does not stay in focus The focusing drifts downwards or can be moved only with difficulty If an electrical fault develops first check the following The image Is too dark The image on the screen is too dark Blurred photographs Color photographs have a yellow cast 98 Tips and hints What to do if Remedy e Setthe interpupillary distance correctly page 15 Locate the eyepoint page 15 Remedy e Fit the eyepieces co
13. light bright field or in clined transmitted light Transmitted light bright field Inclined transmitted light Use Two 160 mAT fuses for voltages between 200 240 V Two 315 mAT fuses for voltages between 100 120 V see the section Starting up on the left gt Disconnect the power cable from the stand gt Pull out the fuse holder gt Fuse at rear Press out downwards gt Spare fuse at front Pull out sideways and insert into holder at rear gt Push the fuse holder back into position e Connect an incident lamp to the transmitted light stand if required e Either transmitted light or incident light can be switched on gt For transmitted light Switch in position l gt For incident light Switch in position ll gt Use thumb wheel to adjust brightness gt On the rear of the stand Use the thumb wheel to switch on the transformer and to adjust the brightness gt Switch on transmitted light Bright field is suitable for examining translucent objects featur ing contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background gt For transmitted light bright field Pull lever forwards E ae Inclined transmitted light which traverses the object obliquely will produce effects advantageous for observing small translu cent objects such as foraminifera and fish eggs Move the lever slowly towar
14. 0 32x Achromat 10 445 352 32 Spacer rings 10 445 614 MZ6 10 446 275 1x Plan n 10315306 Optics carrier optical accessories Ob eetive eom binations Spacer rings are required in order to attach the objectives for certain instrument configurations e The Leica MZ95 is supplied with a spacer ring 10 446 393 for the smaller diameter achromatic objectives and 1x planachromatic objective When the spacer ring has been removed by applying the tool provided the larger diameter planachromatic and planapochromatic objectives for the MZ125 MZ16 can be used e The Leica MS5 MZ6 and MZ75 can also be equipped with the larger diameter planachromatic and planapochromatic objec tives for the MZ125 MZ16 if a spacer ring is fitted 10 446 172 e When the MZ125 MZ16 planachromatic and planapo chromatic objectives are used on the Leica MS5 MZ6 MZ75 and MZ95 the magnification is increased by the factor 1 25x 10 446 370 MZ12s 10 446 272 MZ9s 10 422 564 C 10 447 148 C Log po c j c jJ p Jj b 10 447 157 1x Plan 10 411 589 1x 10 446 275 1x 10 446 157 0 5x Plan 10 422 562 1 5x Plan 10 447 075 0 8x Plan g gt g e 10 422 561 2x 10 446 230 1x Planapo CHR ES 10 446 172 10 473 832 0 8x 10 447 101 2x Planapo gt A 10447148 10445 201 0 63x 10 472 650 1 6x Planapo 3 0 4 0 63x 10 422 563 0 5x 10 446 236 0 63x Planapo E 1 Ergo 10 422 564 0 32x Plan Planapo TR Achromat x 10
15. 0W 24 Transmitted light stand bright and dark field 26 TRARBETIERS CTE StH HL wou cn oassssesme vsus a ez 2 Swinging arm stand ESD sasae e assan omm mmemr 28 Large swinging arm and table clamp stands 30 Optics carrier optical accessories Spacer rings objective combinations 32 PAIGTOSCGNG CAPO ccs ias wee kcu uxadahusscoER EE SA banii 34 senssa osya a ARE 34 Binocular tubes optical accessories susessss 35 Objective nosepiece MZ16 and MZ16 A 36 Leica M Stereomicroscopes User Manual Page Illuminators Transformers a suveke ek ERR MER EEK G18 os REO CR KE RB SORE 38 leiden FTG NIU VE eiui eaa vec E mi cto 40 Incident lamp 6 VZD W 65s ssccecceezesas AERE ER caus aon 42 eb MEER oancssiid sni QUEDA Adan qa nz nj rel 45 Fibsr antig EN oss veces vas we a a aw OREN TER RE 46 LED ONIGEHEEUSD auesxscsvssssuDET EE Es 229 Ps See Ev 166a acm 46 Supply Hirekhanp28 WM o eaw senex aa rmm m rmm mmm 47 Coaxial lumia uso saa a mee na uaa 48 ERR res rime 4 Near Vertical illamirnator sasas mememe mmm 48 Accessories Filling accessory TUDES ea ncis ki isasa eR ates es RHET 50 Donnie me HIHBRIEBE ioo emm ipta na deer re xs ute 50 Drawing tube discussion tube cee eee ee eee ees 51 Video phototubes Photography video TV filming digital imaging 52 Attachment for vertical and ob
16. 16 A Display of magnification factors from 0 71 11 5 MZ16 A For the digital display of the magnification see the user manual M2 116 0 The tables on pages 62 66 provide information about the magnifications and field of view diameters as a function of the magnification changer position of the eyepiece objective combination used When the MZ12s MZ16 planachromatic and planapochromatic objectives are used on the Leica MS5 MZ6 MZ7s and MZ9 the magnification is increased by the factor 1 25x This factor is taken into account in the tables MZ12s MZ16 MS5 MZ6 MZ7s MZ9s 1 OX Leica M Stereomicroscopes Operation 13 Preconditions for working comfortably Ergonomics Viewing height viewing angle Ergonomics viewing height e Pay great attention when setting up your stereomicroscope You will only benefit fully from the outstanding optical and ergonomic advantages of the instrument if you set it up precisely in accordance with the directions already given For fatigue free work use the ErgoModule Arrange your workplace as well as possible and pay attention to the heights of chair and table When sitting use the entire seat area and also the back support of the chair Support your forearms Please also observe the note on p 7 The reasons why users often cannot work comfortably with the 45 tube are the differing heights of the outfits the various accessories and working distances and the
17. 1x Planapo 55 Planapo 19 Planapo 27 Achromat 97 Planapo 89 Achromat 112 Achromat 112 Plan Eyepieces Magnification changer position Total magnification Field diameter mm Total magnification Field diameter mm Total magnification field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Position 6 for MZ95 only When using the planachromatic and planapochromatic objectives MZ125 the magnification is increased by the factor 1 25x 64 Leica M Stereomicroscopes User Manual Working distance mm 63 5 mm Magnification changer position Total magnification Field diameter mm Total magnification Field diameter mm Leica M Stereomicroscopes User Manual 65 Optical data of Leica MZ125 MZ16 MZ16 A Dimensions of Leica MS5 Objectives 0 63x Planapo 0 8x Plan 1 6x Planapo 2x Planapo 1x Planapo Eyepieces 60 Plan 135 Plan 97 Planapo 19 Planapo 15 Planapo 55 Planapo 10x 21B Zoom positions 0 71 and 11 5 only for MZ16 MZ16 A with incident light stand with transmitted light stand Magnification changer position Total magnification Field diameter mm Total magnification Field diameter mm Total magn
18. 30 1 905 762 89 37 8522 564 4163 45 44 5401 11 33 1 4732 8586 49 6251 136 155 39 02 5740 3273 81 3 543 596 15 82 2 514 6548 31 70 41 32 109 35 1 213 854 668 65 6 77 30 628 34 93 494 9532 46 8 625 45 10 41 1809 34 44 44 1908 609 992 1 847 405 0164 and representatives of Leica Mierosystems in more than 100 countries The Business Units in Leica Microsystems hold the management system certificates for the international standards ISO 9001 and ISO 14001 relating to quality management quality assurance and environmental management Leica Microsystems Switzerland Ltd Business Unit SM CH 9435 Heerbrugg Telephone 41 71 726 33 33 Fax 41 71 726 33 99 www leica microsystems com www stereomicroscopy com Ihe companies of the Leica Microsystems Group operate internationally in five business segmenta where we rank with the market leaders Wivi Our expertise in microscopy is the basis for all our solutions for visualization measurement and analysis of microstructures in lilo sciences and industry D HN G hy lt praratien We specialize in supplying complete solutions for histology and cytopathology masing Systen With confocal laser technology and image analysis systems we provide three dimensional viewing facilities and offer new solutions for cytogenetics pathology and material sciences Medical quipimen Innovative technologies in our surgical micro scopes offer new therapeut
19. Leica Microsystems the brand for outstanding products Leica Microsystems Mission is to be the world s first choice provider of innovative solutions to our customers needs for vision measurement lithography and analysis of microstructures Leica the leading brand for microscopes and scientific instruments has developed from five brand names all with a long tradition Wild Leitz Reichert Jung and Cambridge Instruments Leica symbolizes not only tradition but also innovation Leica Microsystems an international Company with a strong network of customer services Australia Gladesville NSW Tel Austria Vienna Tel Canada Richmond Hill Ontario Tel China Hong Kong Tel Denmark Herlev Tel France Rueil Malmaison C dex Tel Germany Bensheim Tel Italy Milan Tel Japan Tokyo Tel Korea Seoul Tel Netherlands Rijswijk Tel Portugal Lisbon Tel Singapore Tel Spain Barcelona Tel Sweden Sollentuna Tel Switzerland Glattbrugg Tel United Kingdom Milton Keynes Tel USA Bannockburn Illinois Tel 1 800 625 286 43 1 486 80 50 0 1 905 762 20 00 8522 564 6699 45 44 5401 01 33 1 4732 8585 49 6251 1360 39 02 57 486 1 81 3 543 596 09 82 2514 6543 31 70 41 32 130 35 1 213 889 112 65 6 77 97 823 34 93 494 9530 46 8 625 45 45 41 1 809 34 34 44 1908 666 663 1 800 248 0123 Fax Fax Fax Fax Fax Fax Fax Fax Fax Fax Fa Fa x x 61 2 9817 8358 43 1 486 80 50
20. The following must be observed if Leica products are build into third party products The manufacturer of the complete system or the person putting it on the market is responsible for adhering to applicable safety regulations laws and guidelines Adhere to general and local regulations relating to accident prevention and environmental protection The products described here must be disposed off in accor dance with applicable local laws and regulations The electrically operated accessories for the Leica MS5 MZ6 MZ7s MZ9s MZ12s MZ16 and MZ16 A stereomicroscopes are constructed in accordance with the latest technologies and provided with a statement of conformity with EC requirements Leica M Stereomicroscopes Safety regulations AN A i Symbols used in this user manual Safety instructions This symbol denotes information which must be read and obeyed Non observance can put personnel at risk can cause malfunctioning or damage to the equipment Warning about accessible hot places e g bulbs Failure to respect it can cause burns Important information This symbol denotes additional information or explanations which promote understanding Action gt This symbol within the text indicates that certain operations must be carried out Explanatory notes This symbol within the text stands for additional information and explanations Leica M Stereomicroscopes Safety regulations verview St
21. an be fitted to the horizontal arm in either of two positions see page 34 e The circular port in the microscope carrier accepts an illuminator gt Insert the peg of the inclinable focusing drive into the connecting piece gt Retighten the clamping screw gt Fit the optics carrier in the microscope carrier gt Turn the optics carrier sideways in the microscope carrier as required gt Retighten the clamping screw Leica M Stereomicroscopes Assembly Focusing Tilting Altering the working distance Sideways movement Altering the overhang The focusing knobs are located on the inclinable drive housing Is the focus movement too loose or too tight Does the outfit tend to slide downwards You can adjust the movement in accordance with the weight of the outfit and with your own personal requirements gt Hold the drive knobs firmly with both hands and turn them in opposite direc tions until you obtain the required degree of resistance during focusing Possible adjustments gt Release the clamping screw gt Tilt the instrument gt Retighten the clamping screw gt Release the clamping screw gt Raise or lower the cross member as re quired together with the horizontal arm gt Retighten the clamping screw Reposition the safety ring see page 28 gt Release the clamping screw gt Move the instrument sideways on the cross member gt Retighten the clamping screw gt
22. and bright field 20 W Follow the safety instructions given on page 7 gt Fit the side faced column to the transmitted light base as described on page 23 Never touch the bulb when it is hot You may sustain burns Do not touch the new 6 V 20 W halogen bulb directly with your fingers because finger marks can cause it to crack when heated Remove finger marks immediately with a cloth dipped in pure alcohol gt Insert the slider together with the bulb socket gt Hold the new bulb in a cloth and push it into position gt Push the slider carefully but complete ly back along the rail to the stop he transmitted light stand contains a KG1 filter You can insert other 50 mm filters if you need them gt Pull out the slider gt Insert the 50 mm filter gt Push the slider carefully but completely back along the rail to the stop The ex factory setting of the voltage selector is 230 for volt ages between 200 240 V and the fuse holder contains two 160 mAT fuses For 100 120 V power supply gt Set the voltage selector to 115 gt Fit the enclosed two 315 mAt fuses in the fuse holder instead of the two 160 mAT fuses see the section Changing fuses on the right gt Connect the power cable to the socket of the base and to the power source Leica M Stereomicroscopes Assembly Changing fuses Transmitted or incident light Switching on the transformer Transmitted
23. anging fuses In the event of overload AN You can prolong the life of your bulb if you gt First switch off the lamp by turning the rotary knob into the black range gt and only then switch the transformer on or off gt Turn on the power switch The diode is illuminated gt Use the rotary knob to adjust the brightness Above 6 V the diode is illuminated Fuses 1 0A 5x20 stock no 167 651 gt Lamp off gt Power switch off gt Turn the fuse holder counterclockwise and extract it gt Fit a new fuse and replace the fuse holder gt Lamp off gt Power switch off gt Wait a few seconds gt Power switch on gt Lamp on Step transformer Follow the safety instructions given on page 7 You can attach a 6 V 10 W lamp to the step transformer gt Set the voltage selector on the rear side to 115 V or 230 V gt Attach the power cable to the power supply gt Connect the lamp gt Adjust the brightness with the three step switch 4 5 6 V Leica M Stereamicroscopes Illuminations 39 Assembly Lampholder gt clamp for side faced columns Lampholder gt cast foot Lampholder gt objective Lampholder gt inclinable focusing drive Lamp housing 3 lampholder arm 40 6 V 10 W incident lamp e The 6 V 10W lamp is an inclined incident source for illuminating spatial objects By using two lamps you can soften or eliminate shadows e At the nomi
24. apochromatic objective Verity that the object is in focus at highest magnification with the 2x planapochromatic as well as the 1x planapochromatic objective without refocusing Otherwise turn the objective at the ring nut until the object is focused gt Fasten the ring nut with both screws Do not overtighten bcth screws Tec S x a Ge m Leica M Stereomicroscopes Assembly 37 38 Starting up Transformers Follow the safety instructions given on page 7 Leica transformers may only be opened by authorized service personnel and the power cable must be disconnected before hand The transformers are not to be used outdoors Regulating transformer The regulating transformer 0 7 V 40 VA is used to operate the 6 V 10 W and 6 V 20 W lamps Voltage selector 2 Connections for two lamps maximum combined loading 40 W 3 Connection for power cable Fuse holder F1 0 A Power switch on 0 off ON display Instrument is switched on Regulating knob 6 V display The maximum voltage has been reached c P co C gt Set the available voltage on the voltage selector Position 115 for 100 120 V Position 230 for 200 240 V gt Connect the power cable to the socket of the base and to the power source gt Connect the lamp The sockets accept two lamps with a maximum combined loading of 40 W Leica M Stereomicroscopes Illuminations Switching on and off Ch
25. ch the 6 V 20 W halogen bulb directly with your fin gers because finger marks can cause it to crack when heated Remove finger marks immediately with a cloth dipped in pure alcohol gt Press the spring clips together and pull off the rear part of the housing gt Hold the new bulb in a cloth and push it into position gt Press the spring clips together and close the housing again gt Center the bulb The 6 V 20 W bulb is a powerful source of illumination The more carefully you set it up the more uniform the lighting will be For this reason recenter a new bulb after fitting it gt Switch on the lamp gt Swing out the diffusing filter gt Pull the condenser lens fully out gt Hold the lamp and release the clamping screw gt Point the lamp vertically downwards at a white sheet of paper so that two filament images are seen gt Retighten the clamping screw gt Using the slotted screw bring the two filament images into focus gt Displace the filament images so that they are opposite to one another but in contact gt Swing back the diffusing filter Leica M Stereomicroscopes Illuminations o i Adapter gt 300 mm focusing drive Adapter gt baseplate Leica L2 cold light source The antistatic Leica L2 cold light source is powerful small compact inexpensive and suitable for simple observation tasks with the Leica MS5 and MZ6 stereo microscopes In addition to one or
26. d operations Do not fit different plugs Do not dismantle optical systems or mechani cal parts unless instructions for doing so are given in the user manual The Leica MS5 MZ6 MZ7s MZ9s MZ12s MZ16 and MZ16 A stereo microscopes are intended mainly for indoor use e fthe stereomicroscope is used outdoors protect it from dirt and mois ture Lamps and electrically operated stands from Leica may not be used outdoors The optics carriers MS5 MZ6 MZ7s MZ9s MZ12s MZ16 and MZ16 A the binocular tube ErgoTube M ErgoWedge 5 25 the swinging arm stand ESD and the cold light sources Leica L2 and CLS consist of ESD discharging material surface resistance lt 10 ohms squared discharge time 2 seconds 1 000 V to 100 V A connection socket for a 4 mm grounding cable is provided on the microscope carrier There are two connection sockets for 4 mm grounding cables in the base of the swinging arm stand Repairs may only be carried out by Leica trained service technicians Only original Leica spare parts may be used Ensure that personnel who use the instrument have read and under stood this user manual and in particular the safety instructions e Ensure that the Leica MS5 MZ6 MZ s MZ9 MZ12s MZ16 and MZ16 A stereomicroscopes are operated maintained and repaired only by authorized and trained personnel Leica M Stereomicroscopes Safety concept Liquids Power cables Workplaces with stereomicro
27. ds the column of the stand until the desired effect is obtained Leica M Stereomicroscopes Assem bly 25 Starting up Transmitted light bright field Transmitted light dark field Transmitted light stand bright and dark field The illumination of the transmitted light stand bright and dark field consists of a fiber optic light guide and a light source Observe the safety regulations from the manufacturer of the light source gt Unplug the fiber optic light guide from the connection in the stand gt Fit the side faced column to the transmitted light base as described on page 23 gt Fit the microscope carrier and stereomicroscope as described on page 34 gt Attach the fiber optic light guide at the stand and the light source Before using the light source read the corresponding user manual Bright field is suitable for examining translucent objects featur ing contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background gt Slide handle to the right until the stop bright field Dark field offers more information for objects poor in contrast with rather poorly marked or very fine structures In this case the light beams penetrate the object at a flat angle Fine detail structures and contours contrast brilliantly and brightly from dark background gt Slide the handle out to the right dark field Leica M Stereomicrosco
28. ent total magnification and in REF mode it shows the current measurement with objective 1x or 2x A10r2in front of the value indicates the selected objective Remove the securing strap from the objective nosepiece gt Remove both screws at the bottom Leica MZ16 and MZ16 A are delivered with a threaded adapter for objectives Remove the adapter to use the objective nosepiece gt Invert the optics carrier gt Loosen 3 screws and remove the adapter The objective nosepiece features two threads a fixed thread for the 2x planapochromatic objective a thread with 2 Allen screws for the 1x planapochromatic objective with adjustable ring nut The adjustable ring nut is supplied separately and used to adjust the parfocality between both objectives Invert the objective nosepiece to simplify the assembly of the objectives gt Screw on the adjustable ring nut on the thread of the 1x planapochromatic objective until the limit stop and tighten it with the enclosed wrench gt Screw in the 1x planapochromatic objective with the ring nut in clockwise direction on the side with the 2 Allen screws The fine pitch thread on the ring nut consists of 24 threads You should expect at least 40 turns The adjustable ring nut must never be set higher than the top surface of the nosepiece plate to prevent the nosepiece from locking while rotating Leica M Stereomicroscopes Assembly gt Carefully turn and tighten the 2x planapoch
29. epeated fine motor tasks such as focusing by hand can lead to muscle fatigue and hand problems Using the Leica motor focusing system every equipment can be moved up and down without applying force Interpupillary distance eye contact The interpupillary distance is correct when you see a single circular field when viewing with both eyes gt Look into the eyepieces and use both hands to move the eyepiece tubes closer together or further apart The distance between eye and eyepiece measures approx 22 mm for 10x 21B wide field eyepieces for persons wearing glasses and 12 mm for 10x 21 wide field eyepieces gt Slowly approach the eyepieces with your eves until you can see the complete image field without corner cutting Leica M Stereomicroscopes Operation 15 Selecting the degree of eye contact Soft eyecups You do not wear glasses and prefer close contact with 10x 21B wide field eyepieces for persons wearing glasses P Holding the diopter ring turn the eyecup counterclockwise until it is released gt Pull the eyecup upwards gt Holding the diopter ring turn the eyecup clockwise Persons wearing glasses need the whole of the available distance Move the eyecups to the lowest position The 10x 21B wide field eyepieces for persons wearing glasses are supplied with soft eyecups which can be attached to the in tegrated eyecups made of hard plastic to achieve the following Prevent eye
30. ereomicroscope 1 Magnification changer 2 Engaging ratchet positions 3a Focusing drive 3b Coarse and fine focusing Larger knob Coarse focusing Smaller knob Fine focusing 3c Adjustable ring Changes the ease of movement of the coarse fine drive 4 Clamping screw Holds the optics carrier in the microscope carrier 5 Clamping screw Holds the binocular tube or accessory to the optics carrier 6 Interchangeable objective Achromatic planachromatic or planapochromatic Leica MS5 Binocular tube 1 Adjustable eyepiece tubes Interpupillary distance adjustable from 52 mm 76 mm 8 Wide field eyepieces for persons wearing glasses Diopter setting adjustable within the range from 45 to 5 and eyecups adjustable 9 Clamping screws These hold the eyepieces in the binocular tube 10 ErgoTube The viewing angle is adjustable within the range 10 50 11 Built in double iris diaphragm MZ16 MZ16 A only The special functions of the Leica MZ16 A are shown in the user manual M2 116 0 Leica MZ6 10 Leica M Stereomicroscopes verview Leica MZ75 Leica design by Ernest Igl Christophe Apoth loz Leica MZ125 bP penal Leica MZ95 Leica MZ16 Leica M Stereomicroscopes Overview 11 Use 12 Changing the magnification Ratchet steps Magnification changer e Zoom means continuous change of magnification Ratchets can be engaged for certain magnifications see below
31. fact that the same instrument is often used by several people of differing builds This problem can be effectively solved by using the Leica ErgoModule If you have problems with the viewing configuration on your stereomicroscope ask your Leica consultant for the most ergonomic Leica solutions The viewing height is correct when your head and back are comfortably positioned On the ErgoTube M the viewing angle 50 is adjustable from 10 50 gt Holding the eyepiece tubes at their bases swing them up or down see arrow Leica M Stereomicroscapes Operation During work breaks stand up and move around for exercise ErgoModule Ergo objective Motor focus Interpupillary distance Locating the exit pupil ErgoWedge 5 25 ErgoWedge 15 ErgoModule 50 mm The ErgoTube 45 has eyepiece tubes which are 65 mm longer than the standard ones Magnification factor 1 6x MZ16 A The magnification factor can be toggled on and off using the OPT key ErgoModule 30 120 mm Relubrication may be required after several years please send the item to Leica Service The achromatic Ergo objective 0 4x 0 63x can be used to ergonomically and accurately focus in the range of 90 mm 63 5 153 5 mm without changing the viewing height At the same time magnification and working distance can be changed without time consuming change of objectives Frequently r
32. gital or SLR camera Example gt Fit the video photo objective H on the left hand side With this equipment you can for example attach an MPS camera see detailed description in the MPS user manual an SLR camera or even a digital or video camera Example With this equipment you can for example attach a digital or video camera gt Fit the video objective 2 on a Leica DC digital camera 3 with the C mount connection gt Connect the video objective with the mounted Leica DC digital camera on the right output of the video phototube gt Tighten the coupling ring 1 Leica M Stereomicroscopes Accessories 55 96 Inserting the graticule Attachment for vertical and oblique observation e The attachment for vertical and oblique observation enables spatial objects to be observed from above and also from all arcund at an angle of 45 The microscope carrier must be fitted in the lower position 1 page 34 e For MS5 MZ6 Fit the 1x achromatic objective by screwing its adapter ring directly into the optics carrier e Spacer rings are required for the Leica MZ75s MZ95 MZ12s and MZ16 to secure the attachment for vertical and oblique observation to the optics carrier page 32 Only usable with 1x achromatic objec tive A detailed description can be found in the user manual M2 266 Graticules The graticules for length measurements and numbering are fitted in mounts and can be i
33. ials and manu facture It does not however cover damage resulting from careless or improper handling Please handle your valuable optical instrument with care It will then fulfill its function with the same precision for decades Our instruments are known for that If contrary to expectations your stereomi croscope develops a fault please call in a specialist contact your local Leica agent or Leica Microsystems Switzerland Ltd CH 9435 Heerbrugg From moisture vapors acids alkalis and corrosive substances and keep chemicals away from the instruments From improper handling Never dismantle optical systems or mechanical parts unless instructions for doing so are given in the user manual e From oil and grease Do not grease guide surfaces or mechanical parts xo e Leica M Stereomicroscopes Notes 59 Therefore Cleaning polymer components Never clean polymers this way A You can clean polymers harmlessly AN 60 Cleaning Dust and dirt will affect the quality of your results gt Put a dust cover over the instrument during breaks in work gt Use dust caps to protect tube openings tubes without eyepieces and eyepieces gt Remove dust with a pneumatic gt When accessories are not being Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use rubber bulb or with a soft brush gt Clean objectives a
34. ic approaches in microsurgery With automated instruments tor ophthalmology we enable new diagnostic methods to be applied emieonduetar quuipiient Our automated leading edge measurement and inspection systems and our E beam lithography systems make us the firat choice supplier for semiconductor manufacturers all over the world etca MICHOSYSTIMS Illustrations descriptions and technical data are not binding and may be changed without notice M2 105 0en Leica Microsystems Switzerland Ltd CH 9435 Heerbrugg 2002 Printed in Switzerland 1 2003 ROV Leica M Stereo microscopes User Manual etca MICROSYSTEMS Dear User User Manual Thank you for choosing our products We hope that you will en joy the quality and performance of Leica Microsystems products In developing our instruments we have placed great emphasis on simple self explanatory directions In order to utilize all the benefits of your new stereomicroscope we suggest studying this user manual in detail Should you have any questions please consult your local Leica representative You will find the address of the closest local representative as well as valuable informa tion about products and services from Leica Microsystems on our homepage at www leica microsystems com We are gladly at your service Customer service is a big thing with us Not only before the sale but afterwards as well Leica Microsystems Switzerla
35. ification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm 1x Achromat c Measurements in mm 376 314 Additional dimensional diagrams involving Leica ErgoModule are available from your Leica agency 66 Leica M Stereomicroscopes User Manual Leica M Stereomicroscopes User Manual Dimensions of Leica MZ6 with transmitted light stand with incident light stand 1x Achromat Additional dimensional diagrams involving Leica ErgoModule are available from your Leica agency 68 Leica M Stereomicroscopes User Manual BDinensions of Leica MZ75 W with incident light stand with transmitted light stand 380 Additional dimensional diagrams involving Leica ErgoModule are available from your Leica agency Leica M Stereomicroscopes User Manual 69 Dimensions of Leica MZ95 Bllimensions of Leica MZI25 with incident light stand with transmitted light stand JE with incident light stand with transmitted light stand Additional dimensional diagrams involving Leica ErgoModule are available from your Leica agency Additional dimensional diagrams involving Leica ErgoModule are available from your Leica agency 70 Leica M Stereomicroscopes User Manual Leica M Stereomicroscopes User Manual 71 Dimensions of Leica MZ16 with incident light stand with transmit
36. ight of the outfit and with your own personal requirements gt Hold the drive knobs firmly with both hands and turn them in opposite directions until you obtain the required degree of resistance during focusing gt Turn the black ring on the right hand drive knob until you obtain the required degree of resistance during focusing gt Hold the left fine drive gt Turn the right fine drive until the desired resistance during focusing is reached Leica M Stereomicroscopes Operation 17 Microscope carrier for stereoscopic observation Stereoscopic observation Microscope carrier This microscope carrier permits only stereoscopic observation using both beam paths he microscope carrier can be fitted to the drive housing in either of two positions see page 34 e The optics carrier can be turned sideways in the microscope carrier if the user needs to work from the side gt Release the clamping screw gt Turn the optics carrier sideways as required gt Retighten the clamping screw Microscope carrier AX The stereomicroscopes can be equipped with the microscope carrier AX for stereoscopic and axial observation instead of the standard microscope carrier page 34 Different models are available for MS5 MZ6 MZ75 MZ9s and for MZ125 MZ16 and MZ16 A The achromatic objectives and the planochromatic 1x fitin the thread in the AX microscope carrier for MS5 MZ6 MZ7s MZ95 p 33 The pla
37. imen plane changes depending on the position of the mirror so that the transmitted light bright field via oblique illumination can also generate an illumination similar to dark field gt Rotate and move the mirror with the black rotary knob on the right side gt Adjust exact bright field and maximum illumination move mirror to the stand column until it stops Rotate the mirror until it engages in the 45 position Leica M Stereomicrascopes Assembly 27 Assembly of column gt base Safety ring gt column Cross member gt column Horizontal arm gt cross member Drive housing gt horizontal arm Optics carrier gt microscope carrier 28 Swinging arm stand ESD The swinging arm stand ESD is of conducting material surface resistance lt 10 Ohm squared discharge time 2 seconds from 1 000 V to 100 V Two sockets for connecting 4 mm ground cables are provided on the base Insert 4 Allen screws into the base from beneath and secure them to the end of the column The safety ring secures the instrument and must always be fixed beneath the cross member gt Slide the safety ring down the column gt Retighten the clamping screw gt Lower the cross member onto the safety ring gt Retighten the clamping screw gt Slide the horizontal arm into the cross member with the connecting piece towards you gt Retighten the clamping screw e The drive housing microscope carrier c
38. l cause damage to the equipment Inspect cables regularly for damage Defective cables can directly endanger personnel cause the stereomicroscopes and accessories to become electrically live endangering personnel Leica M Stereomicroscopes Safety regulations 7 Cable routing Opening the instrument Supply voltage Connections Changing bulbs Integration in third party products Legal requirements Disposal Conformity with European Community directive Route cables with care Make sure that personnel do not get entangled with them The instrument could fall over fall down become damaged damage other equipment or cause personal injury Only authorized Leica personnel are permitted to repair electri cal equipment Before opening up the equipment pull out the power cable If you touch the live interior of the equipment you may receive an electric shock Ensure that the voltage setting on the equipment is correct A wrong setting can cause damage to the equipment When connecting power consuming devices to the transform ers do not exceed the maximum permitted loading as this can cause damage to the equipment Before changing a bulb disconnect the power cable Never attempt to touch the inside of a lamp housing or transmitted light stand while the instrument is connected to the power supply Before changing a bulb wait until it is cool otherwise you may burn your fingers
39. lamping screw and remove the adapter ring from the incident light housing for near vertical light gt Screw the adapter ring onto the microscope carrier gt Secure the incident light housing to the adapter ring gt First screw the spacer ring s page 32 onto the microscope carrier then screw the adapter ring to the spacer ring gt Secure the incident light housing to the adapter ring gt Screw the achromatic objective see above into position gt Release the left or right clamping screw and change the cover plate accordingly Leica M Stereomicroscapes Illuminations No light gt Attach the fiber optic light guide on either the left or the right To switch on the cold light illuminator refer to the user manual provided with it gt Turn the rotary knob e A movable prism directs the light from the left or right outlet onto the object Leica M Stereomicroscopes Illuminations 49 Accessories Assembly 90 Tubes e All accessory tubes which fit between the optics carrier and the binocular tube are fitted in the same manner e nthe dovetail ring of the optics carrier is a locating screw which must engage the notch in the accessory page 35 This is best accomplished by moving the accessory slightly from side to side gt Release the clamping screw gt Insert the accessory tube into the dove tail ring in the same manner as the binoc ular tube page 35 and move it slight
40. lique observation 56 DENEN LL ooosadicgddeus cx nnde Rak MPO orien 8 vU HP EMEN 56 Stages polarization cscs sss use ks vaxuo ania K TREM 57 Special notes Tips and hints Whatto do IF i ee mmn 58 Caro tl aps NICE Loose sena vow winced vm RC oe 59 Calculating total magnification and field of view diameter ssses ee eee eens 61 Optical data for MS5 MZB ice o memora mame 62 Optical dard Tor HZES MESS tcc cosa vcs Westman nan ee 64 Optical data for MZ125 MZ16 MZ16A 66 PUE ener Et ee ee 67 Leica M Stereomicroscopes User Manual 5 General directions Permitted uses Prohibited uses Place of use Use in ESD protected rooms Servicing Responsibilities of person in charge of instrument Read the user manual and safety instructions before starting up The Leica MS5 MZ6 MZ7s5 MZ9s MZ12 MZ16 and MZ16 A stereomicro scopes are optical instruments which use magnification for improved visualization of objects their details and specimens from technology and natural sciences The basic outfit which includes a stand and a light source can be supplemented with various modules for photography TV dual station viewing and other applications The use of the instrument in a different manner from that described in this user manual can lead to injury malfunction and damage The Leica MS5 MZ6 MZ7s MZ9s MZ125 MZ16 and MZ16 A stereo microscopes must not be used for eye examinations an
41. low the focusing drive gt Using a fork wrench and the thread con nector attach the lampholder arm to the a clamp on either the right or the left gt Position the lamp gt Retighten the clamping screw The thread connector also enables the lampholder to be connected to the cast foot A total of 4 filter holders with 32 mm filters can be attached A heat absorbing filter is built in gt Push the filter holder complete with diffusing filter onto one of the pegs and swing it in Leica M Stereomicroscopes llluminatiors Operating the lamp Observe the safety notes on page 7 Illuminating the object Observe the notes with respect to the use of the transformer on page 38 gt Connect the cable between the lamp housing and the trans former page 38 gt Switch on the transformer and use the rotary knob to adjust the brightness gt Push the condenser lens forwards or backwards until the slider engages gt Hold the lamp housing and release the clamping screw gt Align the light spot with the object gt Retighten the clamping screw gt Adjust the size of the light spot by moving the condenser lens forward or back gt If necessary swing in a filter page 42 Leica M Stereamicroscopes Illuminations 43 Changing the bulb Disconnect the lamp cahle from the transformer Centering the bulb Never touch the bulb when it is hot You may sustain hurns Do nottou
42. ly from side to side until the locating screw engages the notch gt Retighten the clamping screw gt Fit the binocular tube on the accessory tube in the same manner Double iris diaphragm A double iris diaphragm is built into the MZ16 and MZ16 A It is operated by means of a knurled ring A separate double iris diaphragm is available as accessory for the other instruments The double iris diaphragm for MS5 MZ12s N can be inserted between optics carrier and binocular tube or video phototube QD Set the lever to OPEN The double iris diaphragm aperture diaphragm is open The depth of field increases as the lever is moved from OPEN to 1 Remember however that brightness decreases so exposure times become longer resolution deteriorates Leica M Stereom croscopes Accessories Drawing Drawing tube The drawing tube is for both left handers and right handers Fit the mirror on the left hand or right hand side of the optics carrier gt Fit the drawing tube between optics car rier and binocular tube as described on page 50 gt Place a sheet of paper beneath the mirror gt Use a table lamp to illuminate the paper gt Move the lever to the circle gt Look into the binocular tube position the object and focus on it Move the lever to the black spot gt Look into the binocular tube and balance the brightness so that the object the drawing surface a
43. n both eyepieces e 100 in the left eyepiece e 100 in the video photo beam path As shown in the setup diagram on page 52 there are various possibilities for adapting an MPS video digital or SLR camera Example With this equipment you can for example attach an MPS camera see detailed description in the MPS user manual an SLR camera or even a digital or video camera gt Fit the 1x video photo objective Example With this equipment you can for example attach a digital or video camera gt Fit the video objective 2 on a Leica DC digital camera 3 with the C mount connection gt Connect the video objective with the mounted Leica DC digital camera on the right output of the video phototube gt Tighten the coupling ring 1 Leica M Stereamicroscopes Accessories Switchover Light partition for observation Light partition for documentation Camera setup variants Video phototuhe HD gt Use the video phototube HD between the optics carrier and the binocular tube page 50 gt Observation Push lever in gt Documentation Pull lever out e 50 to both eyepieces e 50 in the left hand video photo beam path e 50 in the right hand video photo beam path e 100 to left video photo beam path e 50 to right eyepiece e 50 in the right hand video photo beam path As shown in the setup diagram on page 52 there are various possibilities for adapting an MPS video di
44. nal voltage 6 V the color temperature is 2700 K gt Release the clamping screw gt Unfold the lampholder arm gt Retighten the clamping screw gt Use the screw to secure the clamp to the side faced column either above or below the focusing drive gt Use the connector to attach the lamp holder arm to the clamp on either the right or the left gt Fit the 25 mm adapter to the cast foot gt Using the connector attach the lampholder to the adapter gt Remove the objective gt Slide the adapter over the objective gt Using the connector attach the lampholder to the adapter gt Unscrew a connector from the lampholder Release the clamping screw gt Using the thread secure the lampholder to the focusing drive gt Retighten the clamping screw gt Attach the lamp to the connector on the lampholder arm gt Fit the filter holder complete with KG1 heat absorbing filter to one of the pegs e A total of 4 filter holders with 50 mm filters can be attached Leica M Stereomicroscopes llluminations Operating the lamp Observe the safety notes on page 7 Changing the bulb Observe the notes with respect to the use of the trans former on page 38 gt Connect the cable between the lamp housing and the transformer page 38 gt Switch on the transformer and use the rotary knob to adjust the brightness page 39 gt Hold the lamp housing and release the clamping
45. nd Ltd Business unit Stereomicroscopy www stereomicroscopy com Your instrument is accompanied by a printed English user man ual Additional language versions and information can be found on the interactive CD ROM User manuals and updates are available for download on our homepage at Wwww stereomicroscopy com This user manual contains an explanation of the safety regula tions assembly handling and accessories of the Leica MS5 MZ6 MZ7s MZ9s MZ12s MZ16 and MZ16 A if identical stereo microscopes The special functions of the automated stereo microscope Leica MZ16 A can be found in the separate user manual M2 116 0 Leica M Stereomicroscopes User Manual Table of contents Pago Overview DETHED O ou x5 50 EEEE 6 PER PEE PEPE PI PESEE Sc ain EE PEE E A Be 9 BoE e EFE ETET FETTET TETTEIT 10 Use Changing the magnificati n s s sans anees seers sssaenww ees 12 3 mensne saaa maneran rra a rece on n n QOO ORRSRR OR 14 UE TBBBUIBPV HISUNORN sera eas 65s kteeRE TEE TR aa PEN E E EXE 15 RN Laucasesa cul nido Vie ki We x adir d oi c QU 15 Eie cgrt ct GVCCUDS ioxauxasassaess mower cone cad as MERE ens 16 NI ARRPRH 16 Microscope carrier Stereoscopic and axial observation 18 SRG SU Se vga a vied 9 Fe aem t vn E su A n PEU 20 Assembly Dvergiewc CDmpo TOS iiime e ka sace re xn de x 22 Stand Profile column for incident light and transmitted light stands 23 Transmitted light stand bright field 2
46. nd eyepieces with special optic cleaning cloths and with pure alcohol used protect them against dust of unsuitable cleaning agents and techniques can damage polymers so always observe the following rules Never clean them in an ultrasonic bath The polymer can become brittle and later break Never use corrosive cleaning agents or ones containing acetone Never use solvents for cleaning except for ethanol and isopropanol With warm soapy water wiping down afterwards with distilled water With ethanol industrial alcohol and isopropanol When cleaning with ethanol or isopropanol adhere to the appropriate safety regulations Leica M Stereomicroscopes Notes Calculation Total magnification Mg Magnification of objective and field of view Me Magnification of eyepiece diameter z Magnification changer position q Tube factor e g 1 5x for coaxial incident light 45 ErgoTube 1 6x r Factor 1 25x if the planachromatic and planapochro matic objectives of the MZ125 MZ16 are used on the MS5 MZ6 MZ7s or MZ9s Npgy Field number of eyepiece The field numbers are printed on the eyepieces 10x 21 16x 14 25x 9 5 40x 6 Example Mg Eyepiece 1x Mp 25x 9 5 eyepiece Z Zoom position 4 q Tube factor 1 5x because of coaxial illuminator r Factor 1 25x Magnification down binocular tube Moorvs Mox Mpxzxqxr 1 x 25 x 4 x 15 x 125 1875 x Diameter of field of view Leice M Stereomicroscopes Notes 61
47. nd the pencil are all seen together e In general the paper needs to be illuminated strongly but the object only weakly gt Move the lens mount of the drawing tube forwards or back wards until the drawing surface is sharply imaged gt Now you can draw the feature f you place a graduated scale beneath the object and draw its intervals as well you can easily determine the image scale on the paper Discussion tube At the discussion tube two observers see the same erect laterally correct three dimensional image The assembly and use of the discussion tube are described in the separate user manua M2 263 0 Leica M Stereomicrascopes Accessories 51 Video phototubes 1 2 3 1 2 1 3 SLR Leica 3 Chip 2 3 3 Chip 1 2 1 C Mount CCD Cameras Camera housing MPS30 MPS60 B Mount B Mount C Mount Leica DC Cameras Photaautomat CCD Camera CCD Camera CCD Camera Adapter SLR 3 11541 038 aun 0 55 1 1x 8x 10 445 304 10x 1 10445 305 16x 10 162 226 10 445 541 10 446 261 2 3 0 63x 11541 006 1 1x DC150 lt gt 10404 207 11 541 007 2 3 0 63x 10 445 932 11541 016 1 2 0 5x HU 10 446 174 HV 10 446 194 10 446 121 8x 10 446 131 10x 10 446 132 18x 10 445 324 50 10 446 229 100 EC j Leica MS5 Mz6 MZ7 MZ9 MZ12s 10 445 327 M216 MZ16 A MZ FLIII 10 445 931 10 446 197 10 445 925 52 Leica M Stereamicrasco
48. nochromatic and planapochromatic objectives fit in the thread in the AX microscope carrier for MZ12s MZ16 MZ16 A p 33 In addition the AX microscope carrier features a clamp ing screw for fixing it in one of 3 positions and an aperture which can be turned manually see below Otherwise the use of and switching to the left middle and right position are identical e The optics carrier cannot be turned sideways in the micro scope carrier AX A three dimensional image is required for examining plastic objects and for working gt Shift the stereomicroscope to the central position Leica M Stereom croscopes Operation Axial observation Aperture i The parallax free imaging produces more precise results in photography measuring and polarization gt Shift the stereomicroscope to the left or the right position The object is now located directly beneath either the left or the right beam path When photographing or measuring se lect the beam path above which the pho totube or measuring graticule is located A rotating aperture is build into the AX microscope carrier It prevents interfering reflections during axial observation with coaxial incident illuminator and quarter wave plate e With the AX microscope carrier for MS5 MZ6 MZ7s MZ9s the aperture automatically turns during the moving process With the AX microscope carrier for MZ12s MZ16 MZ16 A the aperture is located underneath the microscope
49. nserted into the eyepieces Aspecial measuring graticule is available for the Leica MZ16 A see the user manual M2 116 e A high precision object micrometer with 50 mm scale 0 1 mm and 0 01 mm graduation allows for calibration corresponding to the selected magnification Preferably measure with the microscope carrier AX in vertical position The measurements are more accurate with out the convergence angle in the stereoscopic image Graticules show boundary lines for all film formats gt Unscrew the insert at the knurled ring gt Clamp the graticule onto the insert with a little bit of pressure Check for correct seating gt Tighten the insert with graticule gt Insert the eyepiece gt Look into the eyepiece Align the gratic ule by turning the eyepiece in the tube and tighten the clamping screw A detailed description can be found in the user manua M2 275 Leica M Stereomicroscopes Accessories Gliding stage Cup stage Thermo stages Stages The 120 mm stages can be used in those incident and trans mitted light stands which have a amp 120 mm port The gliding stage can be moved in all directions and rotated completely gt Place the 2 120 mm gliding stage in the 120 mm port and secure it with the eccentric lever C gt Insert the 120 mm glass stage plate or C S gt the black white stage plate ee e The cup stage can be inclined as required e You can secure objects to
50. oved using the tcol provided The Leica MZ9 is supplied with a spacer ring for the achro matic objectives and for the 1x planachromatic objective When the spacer ring has been removed by applying the too provided the larger diameter planachromatic and planapo chromatic objectives for the MZ12 MZ18 can be used Leica M Stereomicroscapes Assembly 35 36 Preparation Assembling 1x planapochromatic Assembling 2x planapochromatic Objective nosepiece e Only for Leica MZ16 and MZ16 A Hold objectives during assembly and disassembly to prevent them from falling on the stage plate This applies particularly to the 2x planapochro matic objective which is very heavy Remove the specimens from the stage plate The adjustable ring nut with the 1x planapochromatic objective is fastened with 2 Allen screws after the adjustment Loosen the Allen screws if you need to remove the objective Attach the limit stop to the stand column It prevents the 2x objective from hitting the limit stop when the objective nosepiece is rotating if the work ing distance was changed xwhile working with the 1 objective Caution Carefully rotate the objective nosepiece and take care not to pinch your fingers between column and objective or objective nosepiece Assembling objective nosepiece Adjusting parfocal MZ16 A When changing to objective 1x or 2x press the OPT SEL function keys simultaneously The display shows the curr
51. pes Assembly Column Base Illumination Accessory condenser gt Basis Use Tilted mirror Transmitted light stand HL The column seat contains Allen screws gt Remove the glass stage plate gt Invert the transmitted light base and place it on a table gt Hold column against the column seat from underneath gt Insert the Allen screws into the column from the top and tighten them gt Invert the transmitted light base and insert the glass stage plate gt Fit additional components such as the microscope carrier and optics carrier page 34 Add a very luminous cold light source and a light guide with a channel to the transmitted light stand TVC active 2 9 mm gt Insert light guide into the base at the rear gt Connect light guide to the light source For stereomicroscopes with high apertures such as Leica MZ12s MZ16 and MZ16 A with objective 1 6x the resolution can be increased by inserting an accessory condenser gt Push the accessory condenser into the lens holder at the underside of the transmitted light base and secure it with 2 Allen screws gt Turn on the light source according to the manufacturer s user manual and adjust the light intensity gt Place a transparent object on the glass stage plate and focus The built in mirror features a clear and an obscured side and can be rotated and moved The obscured side provides a dif fused illumination The angle of incidence in the spec
52. pes Accessories Partition of light Stop e Video phototubes are designed to support modular photomicrography systems and Leica digital video film or SLR cameras e The modular photomicrographic systems and the Leica digi tal cameras are detailed in a separate user manual along with instructions for their assembly and use When you are not taking photographs engage the observa tion beam path Accessories such as the coaxial incident illuminator always fit between the stereomicroscope and the phototube Video phototube A This tube does not permit stereoscopic observation The image is studied either in the camera viewfinder or down the focusing telescope of the camera In the video photo beam path 100 light is available Trinocular video phototube 5096 gt Fit the trinocular video phototube on the optics carrier in place of the binocular observation tube page 35 For examples on camera assembly see page 54 e 50 to both eyepieces e 50 in the video photo beam path Before taking the photograph close off the right eyepiece to keep out stray light gt Move lever to the left Leica M Stereomicroscopes Accessories 53 Switchover Light partition setting VIS Light partition setting PHOT Camera setup variants Trinocular video phototube 100 Fitting See Trinocular video phototube 50 gt Observation Push lever in gt Photography Pull out lever to the left e 100 i
53. rizontal arm gt Retighten the clamping lever a The clamping levers can be positioned Ire as required gt Pull outthe clamping lever along Its axis and turn it Leica M Ste eomicroscopes Assembly Microscope carrier gt drive housing Optics carrier gt microscope carrier Tilting Focusing with coarse fine drive Altering the working distance Sideways movement Altering the overhang gt Secure as described on page 34 gt Secure as described on page 34 Possible adjustments gt Release the clamping lever gt Tilt the instrument gt Retighten the clamping lever gt Set large distances with the larger knob gt Fine focus with the smaller concentric knob gt Using the ring on the right hand drive knob adjust the ease of movement of the drive gt Release the clamping screw gt Raise or lower the horizontal arm gt Retighten the clamping screw Reposition the safety ring see page 30 gt Release the clamping screw gt Pull out or push in the horizontal arm gt Retighten the clamping screw During the sideways movement observe the limit stops see page 30 gt Release the clamping screw gt Displace the horizontal arm gt Retighten the clamping screw Leica M Stereomicroscopes Assembly 31 10 445 613 MS5 e 10 411 589 1x 10 422 562 1 5x 10 422 561 2x 10 473 832 0 8x 10 445 201 0 63x 10 422 563 0 5x 10 422 564
54. romatic objective clockwise to the limit stop without applying excessive force gt Raise the focusing drive with the drive heads to provide sufficient space for the 2x planapochro matic objective gt Fit the objective nosepiece like a microscope car rier with the Allen screw see page 34 position 1 gt Insert the optics carrier in the objective nosepiece gt Retighten the clamping screw gt Attach the limit stop to the column of the stand so that the working distance beneath the 2x objective will always measure approx 15 mm e Fit and adjust the 1x and 2x planapochromatic objectives as described The image then remains parfocal in the range of 0 05 mm and refocusing after changing the objective is no longer required e Use a very fine flat object e g an object micrometer for the adjustment which allows maximum focusing even at highest resolution gt Screw the 2x planapochromatic objective into the beam path gt Adjusting the diopter settings and parfocality see p 20 gt Screw the 1x planapochromatic objective into the beam path gt Observe the same object with smallest magnifica tion without changing the settings made with the 2x planapochromatic objective gt Turn the 1x planapochromatic objective counter clockwise until the object can be seen in focus at the smallest magnification gt Slowly zoom to the highest magnification At the same time correct the focus by turning the 1x plan
55. rrectly page 35 Adjust your dioptric settings exactly as described page 20 Remedy Adjustthe ease of movement pages 16 29 31 e Is the voltage selector set correctly bright field transmitted light stand page 24 transformer page 38 e Is the power switch on e Is the power cable attached correctly Are all connecting cables attached correctly Are the fuses in order Remedy e Turn up the regulating knob high enough bright field transmitted light stand page 24 transformer page 38 Open the double iris diaphragm of the phototube TV photography Remedy e Adjust the video camera monitor system in accordance with the directions of the manufacturer Referto the remedies listed above under The image is too dark Remedy Focus precisely page 16 Adjust your dioptric settings exactly as described page 20 Remedy Use artificial light film Turn the regu ating knob of the transformer clockwise to the position of maximum voltage bright field transmitted light stand page 24 transformer page 38 Leica M Stereomicroscopes Notes In this section We quarantee the quality Protect your instruments Care maintenance We will show you how to look after your microscope and how to clean it You are working with a high precision high performance instrument Accordingly we guarantee the quality of each of our instru ments The guarantee covers all faults in mater
56. scopes facilitate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Dependent upon the duration of uninterrupted activity this may lead to asthenic trouble and muscular skeletal pain so that suitable measures must be taken to reduce the strain perfect design of workplace see p 14 work content and work process frequently changing activities detailed introduction of the personnel while considering ergonomic and organizational work related aspects The ergonomic optics concept and the design of the Leica M series aim at limiting the strain on the user to the lowest possi ble level Direct contact with eyepieces can be a potential transmission path for bacterial and viral infections of the eye Users should be made aware of the potential risk of infection The risk can be kept at a minimum by using individual eyepieces or attachable eyecups see p 16 Electrically operated items The safety regulations listed below apply to the following items Transmitted light stand bright field 20 W page 24 Supply line lamp 25 W page 47 Regulating transformer 0 7 V 40 W page 38 Step transformer 4 5 6 V page 39 Motor focus separate instructions Motorized Leica MZ16 A Be careful when handling liquids If spilt on the equipment they can cause the stereomicroscopes and accessories to become electrically live endangering personne
57. screw gt Align the light spot with the object gt Retighten the clamping screw gt Swing in the heat absorbing filter gt Enlarge or reduce the size of the light spot by turning the socket of the bulb Disconnect the lamp cable from the transformer Never touch the bulb when it is hot You may sustain hurns You can separate the lamp housing and the bulb socket Line up the arrow head on the mount with its shaft on the housing Do not touch the 6 V 10 W halogen bulb because finger marks can cause it to crack when heated Remove finger marks with a cloth dipped in alcohol gt Turn the socket to the separation position gt Pull the socket forcibly out of the housing gt Hold the new bulb in a cloth and push it into position gt Replace the socket in the housing The socket clicks into position Leica M Stereomicroscopes Illuminations 41 Assembly Diffusing and other filters 6 V 20 W incident lamp The 6 V 20 W lamp is an inclined incident scurce for illuminating spatial objects By using two lamps you can soften or eliminate shadows Atthe nominal voltage 6 V the color temperature is 3200 K ideal for photography Set the regulating transformer to this nominal voltage see page 38 gt Release the clamping screw gt Unfold the lampholder arm gt Secure the lampholder to the lamp gt Use the screw to secure the clamp to the side faced column either above or be
58. ted light stand 226 392 222 8 388 8 Additional dimensional diagrams involving Leica ErgoModule are available from your Leica agency 72 Leica M Stereomicroscopes User Manual TDimensions of Leica MZ16 A with incident light stand with transmitted light stand 1x Planapo Soe 503 226 392 Additional dimensional diagrams involving Leica ErgoModule are available from your Leica agency Leica M Stereomicroscopes User Manual 73
59. two arm light guides corresponding accessories are al so available for the coaxial vertical and transmitted light illumi nation methods The Leica L2 can be directly connected on the stand The light guides can be mounted with arms in the threads on the microscope carrier For instructions on mounting the clamp for fiber optic light guides see page 46 For detailed information refer to the separate user manual Leica L2 M2 288 0 When in use the Leica L2 fiber optic light source must always be locked into an adapter to ensure stability gt Secure the clamp to the 300 mm focusing drive by tightening the screw gt Using the mounting rail slide the fiber optic light source into the adapter fork until it engages When mounted on the baseplate the light source can be used as a stand alone unit gt Using the mounting rail slide the fiber optic light source into the adapter fork until it engages Leica M Stereomicroscopes llluminations 45 46 Fiber optic light guide Cold light source Leica CLS series The Leica CLS series is a high performance line for h gh light intensity within the smallest possible space and flicker free white light with the lowest possible thermal effect on the ob jects The comprehensive line of accessories enables unlimited use The CLS series is produced of antistatic material When using the ring lamp 76 mm on the planachromatic objective 0 8x 80 mm a special adapter
60. umn with drive housing coarse fine Side faced column with drive housing 300 mm or 500 mm Microscope carrier Optics carrier Interchangeable objective Binocular tube Wide field eyepiece for spectacle wearers with eyecups Various ErgoModule or accessories for video photography drawing fluorescence coaxial incident light etc 22 Leica M Stereomicrosccpes Assembly Side faced column gt incident light base Side faced column gt transmitted light base Stand Incident light stand gt Remove the stage plate gt Insert 3 Allen screws into the underside of the baseplate and secure them to the bottom end of the side faced column gt Insert the stage plate To fit the microscope carrier and the stereomicroscope see page 34 Transmitted light stand A Pull the power cable out of the socket in the stand gt Remove the glass stage plate gt Carefully invert the transmitted light base and remove 7 Allen screws from the base gt Lift off the lower part of the base gt Reverse the upper part of the base gt Insert 3 Allen screws into the cover plate from beneath and secure them to the bottom end of the side faced column gt Replace and secure the base gt Insert the glass stage plate gt Fit the microscope carrier and the stereomicroscope as described on page 34 Leica M Stereomicroscopes Assembly 23 24 Fitting the bulb Filters Starting up Transmitted light st

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