SPECTROPHOTOMETER USER`S MANUAL
Contents
1. oe we eg eek Rent ae eae A Pa y Fig 16 5 Select fitness method by pressing Menu Scroll A v Two methods are available Ist order linear Concentration Intercept Slope Absorbance 1 order linear through zero Concentration Slope Absorbance 15 Press Enter to confirm fitness method selection The screen returns to previous menu and the move to Input Factors automatically 6 Press ENTER to enter the screen displayed as in Fig 17 Fig 17 Suppose 1 order linear fitness method is selected and intercept value is 1 058 and the slope 1s 2 588 7 Enter Intercept and Slope value 7 1 To select a numerical number move the flashing cursor to that number The Menu Scroll A scroll cursor to left The Menu Scroll v scroll cursor to right 7 2 Press WL Conc F A to confirm and enter the number to intercept line 7 3 Use CLEAR LOAD to delete erase any incorrect entry 7 4 Press WL Conc F v to alter the polarity of the number 7 4 Check and make sure the intercept value is correct Then press ENTER to confirm and move to the next screen as displayed in Fig 19 Enter the slope value in the same way as the intercept value 1s entered The Slope value in this example 1s 2 588 Press ENTER to confirm and the screen will return to previous menu and the move to Name Curve automatically Fig 20 Note If 1 order liner through 0 is selected there is no need to enter inte2. UNICO S1205 S2100 SERIES SPECTROPHOTOMETER USER S MANUAL V 1 0 United Products amp Instruments Inc 182 E Ridge Road Dayton NJ 08810 732 274 1155 FAX 732 274 1151 TABLE OF CONTENTS General nformati0on 0 0 ccc ccc ccc ccc cece cece ceuceccucuceueccucuueceunegeucnnes Safety Electrical Warning PEOR Eees r nian mates NEENA Radio Intertoto sated a a E T eras PATO CU CHOI A A aa nlite Matakana annie mas NW OHS Prnciplesaieo loro l Unpackine TastrucioOnhS eel SPECINICALIONS A cd ERE EEEE S o EEE E E E E nade neta E ee E tues a eae E NE Operational Panel dada a AE IDESCEIPTION orlando Operation Instruccion nena vagadesadnenaeee Prepare The SpecHtopnoto meteria BASIC IMODE 17 ADS Meat ta vee Ouantitalivo LES ariadna oigan UTILTT Y BY KNOW Pacs E A cbia ds BY Standard cimas ica axes Soon cali Ws Cali DEA ON aint oh oe Success estos Do citas ess Dark GCN ora Sense lait en ida EDITCLO CS iss ies a 8 etna tdi SYSTEM BASELINE seer ea a a eO TUVO OM DD ZA EE A NRA Rese UC tal ptas coreana cb idad o Memory Mand oia datado brete cios EAEE S EN Wile COme CHO a T E EETA PC LINK Wavelensgth Calibration iii a oe E a aE Holmium Oxide Filter Method 0 ccc ccc ccc nce cece ceceeeeeececececees Didy miom Per Mist diarias A DSOLDancew ACCURACY EOS ais Stary A te suse TN Trouble Shooting e555 50 05 3 ioc A E A ad Error Codes Definitions 00 c cece ccc ccc cnc
3. test 1s defined and saved in All Test List but not yet loaded in Run Test you need 13 to load the test method to Run Test before you can run the quantitative tests using such method Load Test method to Run Test Select All Test List at Quantitative menu Selects the test method in All Test List to be loaded to Run Test Press LOAD button to copy and forward the test method to Run Test Exit from All Test List and enter to Run Test to check and make sure the selected test method is listed A Remove Test method from Run Test 1 At Run Test screen scrolling to the left of the test method you want to remove from the Run Test folder 2 Press CLEAR LOAD to remove it Note The test method is still saved in All Test List and can be reloaded as needed Define Establish Test Method Test method curve must be defined and established before quantitative tests can be run This instrument has open platform for you to establish your own test methods curves Such established method will be saved as defined test in All Test List This section describes how to define establish a test method in All Test List 1 At Quantitative menu select All Test List as shown Fig 14 prt i fooeeds Bitar Be i rii E FE eee E FE FE 3 a e een e iri CELTA t e be sr E i fenol e food o farol o ogeee Eini i La A p Up to 200 defined tests can be s
4. Ll Spectral Bandpass 5nm Stray Radiant Energy lt 0 3 E 340 and 400nm Photometric Range O to 125 T 0 3 to 2 5 Abs 9999 to 9999 Photometric Accuracy 0 004 0 5A a RS232 and Built in Printer Optional i 80 240Vac 50 60 Hz 420W x 340D x 180H mm Light Source Tungsten Halogen 8 5kg Shipping Weight 19 lbs Shipping dimension 20 x 16 x 11 inches Specifications for S2100 Series BY Model S2100 Model S2100UV Wavelength Range 325 1000nm 200 1000nm Spectral Bandpass Repeatability Photometric Range O to 125 T 0 to 125 T 0 3 to 2 5 Abs 0 3 to 2 5 Abs 9999 to 9999 9999 to 9999 Photometric Accuracy 0 004 0 5A 0 004 0 5A RS232 and Built in Printer Optional RS232 and Built in Printer Optional Installation 1 After carefully unpacking the contents check the materials with the packing list to ensure that you have received everything in good condition 6 2 Place the instrument in a suitable location away from direct sunlight In order to have the best performance from your instrument keep it as far as possible from any strong magnetic or electrical fields or any electrical device that may generate high frequency fields Set the unit up in an area that is free of dust corrosive gases and strong vibrations 3 Remove any obstructions or materials that could hinder the flow of air under and around the instrument 4 For S2100UV select either 220V or 110V on the voltage selector switch to match you
5. are to test the same sample at other wavelengths repeat steps 2 to 9 for each wavelength 11 For each new sample you analyze repeat steps 3 to 9 Quantitative Test Run Test using pre defined Method Test method curve must be defined and established before quantitative tests can be run This instrument has open platform for you to establish your own test methods curves Such established method will be saved as defined test in All Test List Refer to Define Establish Test Method section in this chapter Follow the procedures below to run quantitative test 1 At main menu Fig 6 scroll to Quantitative and then press ENTER to Select Quantitative test mode as showed in Fig 11 i hs Fig 11 2 Select Run Test Place reference in the sample holder and make sure it is the optical path working position Scroll to the left of the desired test and then press ENTER to confirm 12 Fig 12 The instruments will automatically set to the proper wavelength as defined and blank the reference as displayed in Fig 12 1 Fig 12 2 and Fig 12 3 Press 0Abs 100 T button to re blank Fig 12 3 After Blanking 3 Insert the sample cuvette into the lightpath working position and sample test result 1s displayed Fig 13 Test result readings 4 Press PRINT SAVE and follow the instruction to print or save the test results Note Run Test folder shows the pre loaded tests ready for immediate test run If the desired
6. displayed Fig 27 Since this 1s the first measurement the absorbance is marked A1 If the No of Replics is 3 then place the second solution of STD No 1 in the cuvette holder Pressing ENTER will measure the second solution of STD NO 1 20 and the second measurement value will be displayed at STD No 1 0 18775A2 Repeat the same procedures to measure the third solution of STD No 1 and screen will display STD No 1 0 1876A3 This finishes the measurement of the three solutions of standard No 1 Press ENTER again to average the measured absorbance values and the screen then displays its averaged value 0 1876A 0 1877A1 0 1875A2 0 1876A3 3 This averaged absorbance value will be used for calculation 8 5 Place standard solution No 2 into the cuvette holder in the optical path Repeat step 8 3 to 8 4 to measure standard No 2 Repeat the same procedures for all the standards in sequence 8 6 After all the standards are measured press ENTER to create the curve as shown in Fig 28 and the screen return to the first with at Name Curve 9 Name Curve and Save Curve Refer to Name Curve and Save Curve in By Factor chapter page 17 10 Display and Print Curve Parameters Refer to Dis amp Print Curve in By Known Factors chapter page 18 UTILITY In MAIN MENU Hig 6 move down to the left of UTILITY and press ENTER to go into UTILITY menu as shown in Fig 29 21 on e
7. monochromator where the collimating mirror directs the beam onto the grating The grating disperses the light beam to produce the spectrum a portion of which is focused on the exit slit of the monochromator by a collimating mirror From here the beam is passed to a sample compartment through one of the filters which helps to eliminate unwanted second order radiation from the diffraction grating Upon leaving the sample compartment the beam is passed to the silicon photodiode detector and causes the detector to produce an electrical signal that is displayed on the digital display Both S1205 and S2100 Series incorporate an RS 232C and or USB bi directional port for connecting to a PC for using the UNICO Application Software Unpacking Instructions Carefully unpack the contents and check the materials against the following packing list to ensure that you have received everything in good condition Packing List Unless otherwise specially ordered the spectrophotometer package should include the following items Description Quantity De C MOP NOLO MIG 1G ih seasssssaacnnisusrenaantecdosdan cunasetontonaseaaencsoosses l Power Ol SA l VCS GASS erne E ESSE EE Set of 4 Cuvettes Quartz S2100UV only oooooooooocccccccooo Set of 2 Dast OC Eee se elle Eat l Monoa e r EE E EEE l Built in Printer Optional cccccooononnnnnnnnnocooocccnnnnnnnnnnnnnss 1 Specifications for 1205 II Model 1205 Lo Wavelength Range 325 1000nm
8. 17 page 505 L Make up N 100 sulfuric acid as the solvent and use part of it to make a solution containing 120 0 5mg litre of potassium dichromate Wash out a square cuvette with solvent and fill with solvent Put the cuvette into the sample compartment and close the lid Select BASIC MODE and Set the wavelength to 350nm Set the reading to 0 000A using the OAbs 100 T key Empty the cell Wash out with dichromate solution and fill with dichromate solution Put the cuvette into the sample compartment and close the lid Read the absorbance of the standard from the LCD display The value should be Calibrated Value 0 004A Refer to the notes above when interpreting the result A he ee Note It is recommended that you refresh the Dark Current refer to Page21 before check Stray Light Check Specification Less than 0 3 T at 340nm by ASTM E 387 A good indication as to whether the stray light level is within specification may be obtained as follows 1 Set the wavelength to 340nm Ze Select BASIC MODE With the sample compartment empty close the lid and press the OA 100 T key to set the LCD display to 100 0 3 Prepare a solution containing 50gm L of sodium nitrite NaNO gt in distilled water and fill a square cuvette with this solution 4 Place the cuvette in the sample compartment Close the lid The display should read lt 0 3 T Note It is recommended that you refresh the Dark Current refer to Page21 before check Troub
9. 5 1 2 X 5 4 sle i se 3 38 EIA Par begin a 3 H a a i hn ss se oe rio oe E Etoo on oses Cosoi E ose osos oo ooed OM oaet Sook En E oere mee eee of of rhe pume E Foret SEE Soo mee ont e a Pd u 2 e kamad eec ceo E coo Psp ee HE Eta eee o reer gee Ses abe cece o ests A E AO Soe ae a EE Daal SEA Y con ee Set wavelength by pressing WL Conc F Press Enter to confirm wavelength selection The screen returns to previous menu and the move to Conc Unit automatically Select concentration unit by pressing Menu Scroll A v Press Enter to confirm Conc Unit selection The screen returns to previous menu and the move to No of STDS automatically Select number of standards which will be used for establishing the test curve Up to 8 standards can be used To select a numerical number move the flashing cursor to that number The Menu Scroll A scroll cursor to left The Menu Scroll v scroll cursor to right Press WL Conc F A to confirm and enter the number Use CLEAR LOAD to delete erase any incorrect entry Check and make sure the number is correct Then press ENTER to confirm and the moves to the next screen No of Replics automatically If you have more than one solution of the save standard concentration value you can measure all of them and use the averaged value for curve establishment Enter the numbe
10. If the saved wavelength has been out of order WL calibrating will be implemented and then go into Main Menu b It is strongly recommended that taking dark current following the steps below after warmup 1 Select UTILITY in Fig 6 and then select Dark Current in Fig 7 9 a ette 1 1 oote o ooo otte e ww eote ese 8s o o se SE PR LELE j Ka eee ee ooo eooo 4 se va se se s se gosse 2 4 Fig 7 2 Dark Current will be taken shown as Fig 8 1 and be saved shown as Fig 8 2 after pressing EXIT Fig 8 2 Tech Notes It is always a good practice to reset the dark current periodically It is especially important if the instrument is hanging on for a long time or the environment is not stable BASIC MODE T Abs Measure l In Fig 6 scroll to BASIC MODE and then press ENTER to Select basic T Abs mode as showed in Fig 9 If the readouts are not 100 0 T and 0 000A you may press 0Abs 100 T to blank Fig 9 BASIC MODE 2 Select the desired wavelength by pressing the appropriate key At any time that the wavelength is changed blank will automatically be implemented as showed in Fig 10 1 10 Fig 10 2 and 10 3 Fig 10 3 Blank automatically Note Press WL Conc F Ia 1 v to change wavelength at Inm and 5nm interval Press Menu Scroll A v to change wavelength at 50nm interval Make a blank reference solution by filling a clean cuvette with disti
11. S2100 series are single beam general purpose instruments designed to meet the needs of the Conventional Laboratory They are ideal for various applications such as Clinical Chemistry Biochemistry Petrochemistry Environmental Protection Food and Beverage Labs Water and Waste Water Labs and other fields of quality control and research Both S1205 and S2100 Series features a digital display of photometric result easy operation and wavelength range of 325nm to 1000nm for 1205 and model S2100 200 1000nm for S2100UV these instruments are ideal for measurements in the visible wavelength region of the electromagnetic spectrum ultraviolet and visible for the S2100UV Working Principle The spectrophotometer consists of five parts 1 Halogen and deuterium for S2100UV only lamp to supply the light 2 A Monochromator to isolate the wavelength of interest and eliminate the unwanted second order radiation 3 A sample compartment to accommodate the sample solution 4 A detector to receive the transmitted light and convert it to an electrical signal and 5 A digital display to indicate absorbance or transmittance The block diagram below illustrates the relationship between these parts Block diagram for the Spectrophotometer v g o 100 Te e Y 0 4e Ca Y d 4 Light Mono sample Detector Display Source chromator Compartment In your spectrophotometer light from the lamp is focused on the entrance slit of the
12. aved in All Test List Note You can t run any test from All Test List Only run test in Run Test menu Define Establish Test method The 200 un defined tests are originally named To be defined 001 to 200 You can choose to rename any of the un defined locations and define your test in one of the following two ways Define Test by Known Factor Define Test by know Standards 14 Define a test by Known Factors If you know the Factor value you can select By Known Factors to define the test method 1 At Quantitative menu scroll to the left of desired location for saving the test method Press ENTER to confirm In this case we choose To be defined 02 to be defined a As E Le E pmm Fig 15 rA Select By Know Factors by pressing ENTER button Fig 15 Follow the screen step by step to enter data Fig 16 3 Set wavelength by pressing WL Conc F Press Enter to confirm wavelength selection 581nm in this example case The screen returns to previous menu and the move to Conc Unit automatically 4 Select concentration unit by pressing Menu Scroll A v Press Enter to confirm Conc Unit selection The screen returns to previous menu and the move to Fit Method automatically Peso gree 5g e cece gozo coe ose 2 3 Ses 332 coe eed Pee Es e dle 2 2 9 2 erao NEE ot eee eve coed 3 EA ote Pre sary ak ey eE iene E MEA use tee
13. e voltage of the local power supply The power cord shall be inserted in a socket provided with a protective earth contact The protective action must not be negated by the use of an extension cord without a protective conductor Warning Any interruption of the protective conductor inside or outside the apparatus or disconnection of the protective earth terminal is likely to make the apparatus dangerous Intentional interruption is prohibited Whenever it is likely that the protection has been impaired the apparatus shall be made inoperative and be secured against any unintended operation NEVER touch or handle the power supply due to the high voltage The protection is likely to be impaired if for example the apparatus e Shows visible damage e Fails to perform the intended measurements e Has been subjected to prolonged storage under unfavorable conditions e Has been subjected to severe transport stresses Performance To ensure that the instrument is working within its specification especially when making measurements of an important nature carry out performance checks with particular reference to wavelength and absorbance accuracy Performance checks are detailed in this manual Radio Interference For compliance with the EMC standards referred to in the EC Declaration of Conformity it is necessary that only shielded cables are used when connecting the instrument to computers and accessories Introduction The UNICO S1205 and
14. ecucuceccecceuencncucucucucecenes OmWDADA NF HHA E 0 W W YW NNNYNNNYNYNNNNNNNWNNM RI 2 RP hhh 00 D0DDGud e e E 0 NN out DooNoOo N NO 09 General information The apparatus described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory For the correct and safe use of this apparatus it is essential that laboratory personnel follow generally accepted safe procedures in addition to the safety precautions called for in this manual The covers on this instrument may be removed for servicing However the inside of the power supply unit is a hazardous area and its cover should not be removed under any circumstances There are no serviceable components inside this power supply unit For the 2100UV avoid touching the high voltage power supply at all times Some of the chemicals used in spectrophotometry are corrosive and or inflammable and samples may be radioactive toxic or potentially infective Care should be taken to follow the normal laboratory procedures for handling chemicals and samples Safety The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT 1974 Read the following before installing and using the instrument and its accessories The 1205 and S2100 Series S2100 and S2100UV included should be operated by appropriate laboratory technicians Electrical Before switching on the apparatus make sure it 1s set to th
15. ess method for the defined test 1 represents Ist order linear 0 represents Ist order linear through zero Intercept value 1 058 in thise case Slope number 2 588 in this case and Concentration unit ppm in this case 17 ELLEF gere que 33 3353 efe j E AN Note N and M are not part of parameter and have no real meaning and effect to the test The establishment of the defined test 1s complete and saved in the instruments Now you may make final check on all the parameters Press EXIT to return to All Test List menu The To be defined 002 has been replaced with BROMINRE LR as shown in Fig 23 po uy eos eme fono LEST z f LELLE E L j a i J bb a T p on w oon STA eon e 4 o vo eoseges solocos LIO em e enef eeej i seater ne y r rii 1 1 sooje feest ri we Ts LL FE Lk MS Define a Test by Known Standards If you have standards with known concentration values you can establish and define test curve by following the instructions in this section l At Quantitative menu scroll to the left of desired location for saving the test method Press ENTER to confirm i me A ft orf ree rfi f yA Select By Standard by pressing ENTER button Follow the screen step by step to enter data and parameters 18
16. f wavelength is found to be not accurate Use this function to make the proper wavelength correction Select WL Correction Fig 38 Fig 38 Press WL Conc F A v to get the wavelength off set number Press ENTER to confirm Press EXIT to return to previous menu Note The correction will be saved in memory and will not get lost after power off PC LINK S2100 and S2100UV are designed to be operated by Computer with UNICO application software In Fig 7 move to the left of PC LINK and press ENTER the following screen 1s displayed Fig 39 25 Fig 39 When the communication between the instrument and the computer 1s established the computer is in control as shown in Fig 40 Please details of the PC software please refer to UNICO application software manual Fig 40 Wavelength Calibration Normally the S2100 and 2100UV Series spectrophotometer retains its wavelength calibration indefinitely However if the instrument receives a severe shock or is abused use the following methods to check wavelength calibration Please note that this test requires the UNICO Didymium filter p n 2100 116 or the Holmium Oxide filter p n 2100 115 In the filter method the didymium filter has two distinct absorbance peaks at 529nm and 807nm The Holmium filter has a distinct peak at 361nm When the instrument is calibrated properly you will find minimum Transmittance maximum Absorbance at the range 2nm from these peaks No
17. g Open cover of instrument and check the connector J11 S2100 Series or J3 S1205 between CPU board and grating driven motor Check the connector JS on CPU board Call authorized service engineer Check sample holder See if holder is properly positioned and nothing is blocking light path Check to see if light is positioned properly on entrance slit to monochromator Check tungsten halogen lamp Replace lamp if necessary Call authorized service engineer Check sample compartment and remove cuvette or any object blocking light path Place the 4 cell sample holder in the proper position See the lamps is ON or OFF via the small hole in the rear of the instrumen if OFF call authorized service engineer Contact an authorized service engineer 30
18. l procedure and wavelength setting Check wavelength accuracy according to procedure in this manual a proper ventilation a E procedure A mr calibration Error Codes Definitions 29 The Error Codes are the classification for errors detected automatically by the instrument Each code represents different errors that occur during the self calibration or during operation The Error Codes are displayed on the wavelength display and are defined as follows Error Code Definition function Causes Solutions Error 1 Instrument unable to initialize and or locate the lamp S2100UV only Error 2 Instrument unable to initialize and or locate the filter Error 3 Monochromator unable to locate starting position Error 4 Instrument unable to locate 0 order light Error 5 Instrument is unable to set 0A 100 T Energy Low display Check power supply to see if proper voltage is selected Open cover of instrument and check the connector J3 between CPU board and Lamp change over motor Lamp change over motor or lamp initialization switch malfunctioning Check the connector J8 on CPU board Call authorized service engineer Open cove rof instrument and check the connector J2 between CPU board and Filter driven motor Filter driven motor malfunctioning Check the connector J4 on CPU board Call authorized service engineer Monochromator contact switch or grating driven motor WL motor is malfunctionin
19. le Shooting 28 PROBLEM Possible Solution Instrument Inoperative Power cord not connected to Plug instrument in outlet Dead Power outlet Change to a different outlet Internal fuse blown or defective Call an authorized service engineer electronic component IN Improper power setting Set to the main power Instrument cannot set Light beam blocked Check sample holder See if holder is 100 T 0 000A properly positioned and nothing is blocking light path Lamp is misaligned Check to see if light is positioned properly on entrance slit to monochromator Call Technical Service for details 800 588 9776 PF Defective electronic component Call an authorized service engineer Incorrect Bubbles or particles in solution Check sample preparation and analytical Transmittance to procedure Absorbance correlation Defective electronic component Call an authorized service engineer LCD Digital Display Concentration reading frozen Sample Solution too Dark dilute it and does not change Restart measurement procedure regardless of sample concentration N Wrong wavelength setting Check sample procedure and wavelength setting Insufficient sample volume Fill cuvette with more sample instrument Use proper ventilation A procedure ee i ai a or loose wiring o o properly misalignment has moved during transit defective electronic component obtained Wrong wavelength setting Check analytica
20. lled or de ionized water or other specified solvent Wipe the cuvette with tissue to remove the fingerprints and droplets of liquid Fit the blank cuvette into the 4 cell holder and place the cuvette in the slot nearest you Push the rod so that the cuvette is in the light path Close the lid Set 0 000A or 100 T by pressing OA 100 T button Remove the blank cuvette 1f you are testing more than 3 samples Set 1t aside in the case that you may need to reset ODA 100 T later 1 e change wavelength Rinse a second cuvette or more with a small amount of sample solution to be tested 11 Fill the cuvette and wipe it 8 Put the sample cuvette s in the sample compartment Close the lid 9 Read the Transmittance and Absorbance from the LCD readout window If you are reading more than one cuvette be sure to carefully move the cuvette holder to the next position by pulling on the sample holder rod until the holder click into place Be sure to make note of the readout or print for each sample Remove the sample cuvette s Operational Note 1 If you are reading 3 or less samples then place the reference cuvette in the position nearest you and the samples in the next available position This will shorten the time to read samples and minimize the sample handling opening and closing the sample compartment lid etc 2 To save or print the readings please press PRINT SAVE and follow the instruction on line 4 of screen 10 If you
21. low Levels Fast Levels Slowest Cable 1 Select Clock Trimming Fig 30 press WL Conc F A 1 Iv to set the adjustment level Fig 33 Press ENTER to confirm Press EXIT return to Set Clock menu Fig 30 System Baseline It is used for determining the appropriate energy level across the whole wavelength range Fig 34 shows establishing system baseline in progress 23 aa pedo Lie m fea pe e m E AA PAPA Pome Une CO gt EREO ww FEH HH IE i La G RS Current Wavelength Fig 34 Deuterium lamp D2 on off Turn on or off Deuterium lamp It is advisable to turn off deuterium lamp if wavelength 200 to 340 are not going to be use for a while Reset Defaults Reserved for future application Memory Manager It has three functions as shown in Fig 35 A Load Saved data Select Load Saved data in Fig 35 The saved data will all be listed as Fig 36 Fig 36 With next to the saved data interested COBALT in this case press ENTER you can print the data if printer installed and display it on screen or you can send the data to computer Fig 37 24 B Clear User Tests Select Clear User Tests in Fig 35 all established and saved tests curves will be deleted Warning All saved test curves will be deleted PERMANENTLY C Clear Data Memory Select Clear Data Memory in Fig 35 all saved tests data will be deleted Warning All saved data will be deleted PERMANENTE Y Wavelength Correction I
22. r erar co gt g or gt E a s eoocese 2 There are eight functions in UTILITY WLCalibration the instrument will implement a self check and calibration procedure It includes filter positioning lamp positioning and wavelength calibrating dark current resetting Dark Current measure and refresh instrument dark current It is designed for eliminating the error from electronic drifting It is recommended that you refresh and save the Dark Current after the 15 minutes warm up and periodically Edit Clock There are three functions as shown in Fig 30 1 Set Clock Select Set Clock in Fig 30 Press ENTER to cycle through W Week Y Year M Month D Day H Hour M Minute S Second Press WL Conc F A v to change Press ENTER to confirm Press EXIT to return to Edit Clock screen Fig 30 22 2 Display of Date or Time The instrument screen can either display date or time hour min sec Select Display Mode in Fig 30 Press Menu Scroll A v to select Display Time or Display Date Fig 32 Press ENTER to confirm Press EXIT to return to Edit Clock screen fete pate ff f i 3 y EE EEE sep o a oq ci e stoo oS i i i ose segon w 3 Clock Trimming This function is only useful when the clock 1s not running accurately Set the off set level clock trimming to adjust the clock There are 64 levels of adjustments S
23. r local mains voltage supply 5 Turn on the instrument and allow it to warm up for 15 minutes before taking any readings E5232 Port OMMOFF Switch a ma Light View Hale Power Cont hs Fig l Back View of 521004 and 5210007 ONICEF Switch S939 Port Power Cord Inlet Coolme Fan Lamp Change Window Fig 2 Back View of 51205 Operational Panel S2100 SPECTROPHOTOMETER 2 PRINT CLEAR SAVE LOAD WwW Conc F Menu Scroll DIODNI Description of Key Functions CLEAR LOAD Clear number or character when editing in Quantatitive Mode Load saved data or saved curve PRINT SAVE Print data Save data or curve 0Abs 100 T Set0Abs and 100 T EXIT Return to previous screen ENTER Confirm the inputted data or selected item Go to next setup or screen WL Conc F Set Wavelength Enter Standard solution value or Enter Known Factor value Menu Scroll gt Browse the items in the menu Operation Instruction Prepare the spectrophotometer Turn on the spectrophotometer by pressing the Power Switch IO The screen displays sequentially as Fig 4 and 5 Press EXIT to skip 15 minutes warm up After 15 minutes warm up the main menu displays as Fig 7 S2100UV locates lamp and set filter before the main menu displays Fig 5 Note Specific model number displays in Fig 4 and Fig 5 Model Vis 1205 shown in this illustration Fig 6 Note a
24. r of replics in the same way as the number of standards is entered If only one standard solution for each concentration value is used just enter 1 Each standard solution will be measured and the averaged value will be used for calculation Press ENTER to confirm and the moves to the next screen Fit Method automatically Select fitness method by pressing Menu Scroll A v Two methods are available Ist order linear Concentration Intercept Slope Absorbance 1 order linear through zero Concentration Slope Absorbance 19 Press Enter to confirm fitness method selection The screen returns to previous menu and the move to Measure Standard automatically 8 Measure standard solutions 8 1 Press ENTER at Measure Standard The following screen will pop up Fig 25 Fig 25 8 2 Place the cuvette with reference solution in the cuvette holder press 0Abs 100 T to blank After blank is complete it is ready for standard solution measurement starting from STD No 1 Fig 26 8 3 Place standard No 1 in the cuvette holder and make sure it is in the optical path working position Enter the known concentration value Refer to Intercept value entry for data entry procedures Check and make sure the value entered matches the value of the standard No 1 it is usually labeled on the standard 8 4 Press ENTER to measure the standard No 1 The Absorbance of the standard solution No 1 will be measured and
25. rcept 16 se 2 s e se a se ee se o ees ee 8 seer e s a se s a ti 5 s Pobal ES P3 hans a F E Fig 20 8 Name the Curve The name character entry 1s the same way as the entry of intercept and slope 8 1 To select a number or character move the flashing cursor to that number or character The Menu Scroll A scroll cursor to left The Menu Scroll v scroll cursor to right 8 2 Press WL Conc F A to confirm The number or character will be displayed below name curve 8 3 Use CLEAR LOAD to delete erase any incorrect entry 8 3 Check and make sure the name is correct BROMINE LR in this case is shown in Fig 21 Fig 21 8 4 Then press ENTER to confirm the name BROMINE LR in this case and the display will automatically return to previous menu with the at Save Curve 9 Save the Curve defined with the at Save Curve press ENTER to save the define curve Then the moves to Dis amp Print Curve automatically 10 Display and Print out Defined Test Curve parameter with the at Dis Print Curve Press ENTER to display and print if printer 1s connected the defined test parameter The data and parameters include Shown as Fig 22 Defined test sequence number in All Test List No 002 in this example case The wavelength used for the defined test 581nm in this example case The defined test name BROMINE LR in this example case Fitn
26. repeat steps 2 to 5 Repeat step 6 until the wavelength setting reaches 815nm Look for the maximum absorbance reading obtained and this should be found between 805 and 809nm Ifa middle wavelength check is desired set the wavelength to 522nm optional Make sure the cuvette holder is empty in the sample compartment Close the sample lid Set zero Abs by pressing the OA 100 T key The reading should then be 0 000A If not press OAbs 100 T again Remove the cuvette holder and insert the Didymium filter into it Place it in the sample compartment and close the lid Record the absorbance reading on the LCD display 2 14 Advance the wavelength setting by Inm and repeat steps 10 to 13 15 Repeat step 14 until the wavelength setting reaches 536nm Again look for the maximum absorbance reading It should be between 527 and 531nm Absorbance Accuracy Checks Specification 0 004A at 0 5A The absorbance accuracy should be checked against a set of neutral density filters accurately calibrated to the NIST standards Contact your UNICO representative for more information 800 588 9776 An alternative method using potassium dichromate is described below Due to the many factors that might affect the results 1 e temperature bandpass weighing and diluting errors this method is less accurate and should only be used as a guide Reference Johnson E A Potassium Dichromate as an absorbance standard PSG Bulletin 1967 No
27. te that the specific Transmittance values are not important as you are only looking for the wavelength where the minimum transmittance maximum Absorbance occurs Holmium Oxide Filter Method 1 Turn instrument on and allow it to warm up for 15 minutes 2 Select the BASIC MODE 1 Set the wavelength to 350nm 2 Make sure the cuvette holder is empty in the sample compartment Close the sample compartment lid 26 Set zero Absorbance by pressing the OA 100 T The reading should then be 0 000A If not press VAbs 100 T again Remove the cuvette holder and insert the Holmium filter into it Place it in the sample compartment and close the lid Record the Absorbance reading on the LCD display Advance the wavelength setting by 1nm and repeat steps 2 to 5 Repeat step 6 until the wavelength setting reaches 370nm 8 Look for the maximum absorbance reading obtained and this should be found between 359 and 363nm Didymium Filter Method l Le 10 11 12 13 Set the Wavelength to 800 nm Make sure the cuvette holder is empty in the sample compartment Close the sample compartment lid Set zero Abs by pressing the OA 100 T The reading should then be 0 000A If not press OAbs 100 T again Remove the cuvette holder and insert the Didymium filter into it Place it in the sample compartment and close the lid Record the Absorbance reading on the LCD display Advance the wavelength setting by Inm and
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