Spectrophotometry Sops
Contents
1. You can send data to a printer or a memory device using the available USB ports Since the system automatically senses connected devices it sends the data to all available devices f Single point To send data FE Filtered data 3 n 1 Touch the Options button C All data 88 2 Touch Send Data USB Port icon The Send Data screen i displays enabling you to send a single point selected item the filtered data or all data to the desired destination s by m Cancel touching the respective radio button Sse PE HA 3 Touch OK to send the data NOTE The number in brackets shows the total number of data assigned to this selection The instrument automatically sends the data as a single DATALOG file to the memory device using the CSV Comma Separated Value file format The name uses the following format DL Year Month Dav Hour Minute Second csv m NOTE CSV files are the preferred format for data import into spreadsheet _ 8 50x11 00im 4 i EE Le EE eee e ess SSeS TT IID tee Pis FM N 0140015 e a note gadget Bmorrison on m I Morrison Prot fc fel Microsoft Pow It http users e mI 7 m Spectrophotometry DU 730 Print Send Scan or Recalled Data Address g http users edutdepartrnients fybiol Spectrophotormeter USOD Manual pdf Links Customize Links Free Hotmail Windows Lo W2. User Programs Wavelength Scan Kinetics Time single Compo nent Protein Assay Analysis NIU OE Nucleic cid Analysis More Select Instrument Setup to verify or reset basic features like the use Of a carousel or not date time etc STLCC CPLS Morrison 6 12 2015 Page 6 Spectrophotometry DU730 Instrument Setup Menu LE s Instrument Setup Operator ID Sample ID lt Off gt lt Off Date amp Display amp di Time Sound O Lamp ur USB Port Control zum E cm Carousel Password a E Select Carousel Options to see previous settings and or reset for this analysis Return STLCC CPLS Morrison 6 12 2015 Page 7 Spectrophotometry DU730 Carousel Option Settings Bert Ai Carousel Options f fF Blank 1 Read 1 4 Blank 1 4 Read 1 4 amp Verify or reset use of Carousel on or off using this toggle and up down arrows C Blank 1 Read 2 4 Pe C Blank 1 3 Read 2 4 ie aa _ Cells V A te oe Bi Vir 1 94 O Vo i Carousel Options Carousel Off for use with a single sample for each analysis Select 1 4 OK when desired status is set Qz Blank 1 4 Read 1 4 y C Blank 1 Read 2 4 A C m EU t he ed STLCC CPLS Morrison 6 12 2015 Page 8 Spectrophotometry DU730 Nucleic Acid Example Select Options Nucleic Acid Analysi
3. a 4 oe Se T T A a TA a n V Um ne ai ThermoSci Genesys 10S UV Vis x 4 BR Spectrophotometry Bio Rad SmartSpec Plus BR Beckman DU 730 UV VIS at BRDG x 3 530 UV VIS At FV opens Prepared by Bob Morrison Nanodrop BR x 2 FVCC Instrumentation Specialist Page 1 STLCC CPLS Morrison 6 12 2015 Original May 2008 Last Revision June 2015 add Genesys d Spectrophotometer Service Maintenance Recommendations Ace Lab Systems 1550 S Kingshighway St Louis MO 63110 Tel 314 771 7272 Fax 814 771 6956 Email Tammy tammyc AceLabSystems com Nov 2010 The Electromagnetic Spectrum X rays Ultraviolet Visible Light Infrared 100 200 280 315 400 780 Wavelength nm Hg low pressure spectral curve of lamp 254nm cell inactivation STLCC CPLS Morrison 6 12 2015 Page 2 On off Toggle Upper Left Rear Panel Main Menu Control Panel Physical and Environmental Width 45 cm 17 7 in Height 20 cm 7 9 in Depth 50 cm 19 7 in Weight 15 5 kg 34 2 Ibs Operating Requirements 10 to 40 C 50 to 104 F max 9096 relative humidity non condensing Power and Interface Connections Power 100 to 120 V 200 to 240 V 50 60 Hz automatic changeover Ports USB 1 1 Spectrophotometry DU 730 Description Save Print Data Right rear panel USB Ports 2 type A 1 type B Flashdisk required Cell o
4. to inspect and or reset previous settings on items like use of the carousel On the Main Menu screen select the general category of use Nucleic Acid Protein Assay etc Follow the Menu options to enter dilution factors and other setting values UV Lamp Warm Up is required before reading blank and samples Observe UV VIS box in upper right The UV will blink until the lamp is warmed up and ready for Blank and Sample reading Insert cuvettes making certain that the V symbol or face is pointed toward the front of the device The UV VIS light path is front to back in the cell Ensure that the lid is totally closed after inserting removing cuvettes stray light can void results Page 4 Spectrophotometry 00730 Lid Loading cuvettes The Electromagnetic spectrum Ultraviolet Visible Light Light path front to rear 400 760 Wavelength nm Hg low pressure Spectral curve of lamp 254nm cell inactivation Slide to door lid toward the rear to access the single or carousel adapter for your cuvette Make sure that the V symbol or indicator on the cuvette for the light path is facing forward Securely Close lid after inserting cuvette s 170 2510 Pkg of 50 individually packaged disposable UV transparent cuvettes DNase and RNase free volume range 50 1 500 ul Page 5 Spectrophotometry DU730 Main Menu Select Instrument Setup Main Menu us Fixed Wavelength
5. Checks pass or fail on the screen Prepare Blank Buffer and Sample careful not to touch the clear sides of the cuvettes Label the stopper for each S sample or B blank 9 Use the arrow keypad below the screen displays to select the Assay and or to set other options and values 6 UV Lamp Warm Up REQUIRED Select Nucleic Acid then Select Assay 1 260 280 Ratio Observe UV VIS box in upper right The UV will blink until the lamp is warmed up and ready for Blank and Sample reading The warm up time can be from 2 10 minutes Do not proceed until the UV display has stopped blinking p 7 The light source is from left to right be sure that the cuvette is placed in the holder cell aligned in this manner 8 Ensure that the lid is totally closed after inserting removing cuvettes stray light can void results Link to Beckman DU 700 series Spectrophotometer User Manual pdf STLCC CPLS Morrison 6 12 2015 Page 34 Spectrophotometer DU 530 Main Menu Optional System Checks On Off Controller Menu Flip up Rear screen Use keypad below Bottom for options Left Corner Optional Select More then System Checks This can be used to self test the system before running Samples Membrane Keyboard Used to enter data and activate displayed items Cell Curvett Lid Swing up to access cuvette and or cell holder fitting Remove Load cuvette or carousel holding cell C
6. Tw d d Spectrophotometer Nanodrop R127 Latest Calibration Test Results Customer Guidance Calibration check is complete Click Reset to begin again or Exit to return to the 3 2 2013 5 32 AM Main Menu o m Target Abs 350 nm 1 0 mm path 0737 1 00 0 90 M Measurementnm 350 Replicates 10 Correction nm 600 1 0 mm Path 0 2 mm Path Target Abs 0737 0147 Z 0 80 Current Abs 1 0 743 0 150 ROP Abs gt _ 0744 0150 8 EM 1 A L Emr 10 16 8 060 CALS CATION TEST __Std Dev 00 0001 Fo s 0 50 m 2 48 TR D 1 mm Path a lt 0 40 MT SELL m c muj 0 2 mm Path Measured Absorbance vs Target zu gt 0 050 gt 0 040 u 0201 2 i aa 0 020 1 1 2 010 Ah R X ow wo x f c S i bsorbance Deviation LC 0 10 i i i i i i I 4 0 040 220250 300 350 400 450 500 550 600 650 700 750 I I I Wavelength nm 1 4 35 4 2808 9 18 38 1 BC183 1 19 128 24 Measurement Page 67 STLCC CPLS Morrison 6 12 2015 Spectrophotometer Nanodrop Solenoid Cleaning Solenoid Cleaning NanoDrop 1000 only Field experience has shown that some brands of lab wipes may shred during the cleaning process and may which could result in a build up of linc under the instrument solenoid A significant build up of lint may alter the abs
7. corresponding to each screen option to activate or set the values STLCC CPLS Morrison 6 12 2015 Page 39 Spectrophotometer DU 530 Main Nucleic Acid Parameters screen Description 2607280 Ratio single Rat iC Double Ratio amp Conc Lamp Box UV will be blinking until the s ae nT istien lamp is warmed up for blank and sample readings a ss DNA RNA 8 Uliqo DNA Long 4 Oligo DNA Short 18 Oligo RNA Long 11 Oligo RNA Short an IM M 0 _ Assay to run 1 11 7 65 PAGE PAGE UP DOWN STLCC CPLS Morrison 6 12 2015 Page 40 Spectrophotometer DU 530 Recommended Warm up and Blank Testing MA TA lt 1 1 Select desired Assay type and follow future screen instructions 2 Note the VIS box in the upper right corner The UV section will blink until the lamp is sufficiently warmed up for Blank and Sample readings 3 Do NOT proceed until the UV blinking has stopped This could take 2 10 minutes 2 single Ratic Double Ratio amp Conc varburg Christ ia Js DNA DNA RNA Jliago DNA Long Oligo DNA Short 19 01 igo RNA Long IU Tl Oligo RNA Short PAGE PAGE UP DOWN STLCC CPLS Morrison 6 12 2015 Page 41 Spectrophotometer DU 530 Main Nucleic Acid Warburg Christian Assay 4 The Warburg Christian method is designed to give the most accurate calculation
8. mL ug mL ug mL ug mL ug mL pg mL pg mL Unit 2 ug mL ug mL ug mL Page 19 Spectrophotometer Beckman DU 530UV Description eCell Module with 1 cm Cell Holder pos eGraphical Liquid Crystal Display eKeyboard and Alphanumeric Pad e100 User Programs eMethods and Data Storage elnstrument Diagnostics eSerial Interface RS 232 eParallel Printer Port eMulti Language Software eFixed Wavelength Mode eScanning Mode e Ime Based Kinetics e Single Component Analysis eProtein Analysis eNucleic Acid Analysis ePerformance Validation Software STLCC CPLS Morrison 6 12 2015 Page 20 Spectrophotometer Themo Sci Genesys 10S UV Vis BRDG Research Quality with Routine Simplicity 1 On Off toggle Accelerate through wavelength scans with scan speeds up to lower rear at power 3 600nm minute line Depend on dual beam optics for superior photometric accuracy Acquire data from the UV to the near IR e Small footprint for easy transport and storage ncrease sample throughput with the integrated 6 cell changer e Thermostatting options with both circulating water and Peltier cooling Measure unusual or challenging samples with a variety of optional holders for test tubes long path cuvettes and filters e Add a sipper accessory for easy sample handling Maintenance Free Lamp e Save time with the instant on xenon flash lamp Perform accurate analysis over the entire wavelength range of 190 1100nm Prevent dama
9. to 800 nm 11 Choose whether to subtract hackeround and if so specify backeround wavelength 111 Choose fast or slow scan iv For the fast scan choose number of successive scans D Kinetic 1 Choose wavelength to read 11 Choose duration of data collection 111 Choose interval between successive readings iv Choose whether to subtract backeround and if so specify backeround wavelength ODD i Accept or modify the conversion factor A 1 Choose number of wavelengths to read 1 Specify the wavelengths to read 111 Choose whether to subtract hackeround and if so specify backeround wavelength Spectrophotometer SmartSpec Quick Start Guide pg 4 3 Ityaur dilution factor is not 1 0 set the dilution factor 4 Zero the instrument Place a cuvette containing the blank solution into SmartSpec Plus and press Read Blank 5 Collect absorbance data Place cuvette contamine sample solution into SmartSpec Plus and press Read Sample Continue collecting absorbance data until all samples are read 6 Absorbance and or concentration data are displayed automatically as they are collected tthere are absorbance data at other wavelengths press Absto see them DNA or RNA oligo concentrations are aralable n units of and pmole ul press Cone to togele between them If absorbance or concentration data area displayed press Enter to return the Ready screen or simply put the next sample cu
10. 280 Ratio 260 280 Ratio 260 280 Ratio 260 280 Ratio Parameter ds DNA ds DNA ds DNA Date Name 2 10 9 2008 9 37 10 9 2008 9 37 10 9 2008 9 37 10 9 2008 9 38 10 9 2008 9 38 10 9 2008 9 38 10 9 2008 9 39 10 9 2008 9 39 10 9 2008 9 39 Date Name 2 10 9 2008 9 40 10 9 2008 9 40 10 9 2008 9 40 Operator ID Wavelength 1 260 260 260 260 260 260 260 260 260 Operator ID ConcX 50 50 50 STLCC CPLS Morrison 6 12 2015 Sample ID Value EILENE 0 072 EILENE 0 071 EILENE 0 071 BOB 0 064 BOB 0 064 BOB 0 063 BECKY 0 016 BECKY 0 017 BECKY 0 016 Sample ID Wavelength 1 BECKY 260 BECKY 260 BECKY 260 Sample Number Unit Abs Abs Abs Abs Abs Abs Abs Abs Abs Sample Number Value 0 066 0 066 0 066 Result 1 Wavelength 2 1 125 280 1 125 280 1 122 280 1 127 280 1 135 280 1 129 280 1 052 280 1 08 280 1 07 280 Result 1 Unit 1 138 Abs 1 132 Abs 1 136 Abs Unit 1 Value Ratio 0 064 Ratio 0 063 Ratio 0 063 Ratio 0 057 Ratio 0 056 Ratio 0 056 Ratio 0 016 Ratio 0 015 Ratio 0 015 Unit 1 Wavelength 2 Ratio 280 Ratio 280 Ratio 280 Name 1 Unit Abs Abs Abs Abs Abs Abs Abs Abs Abs Name 1 Value 0 058 0 058 0 058 Result 2 0 07 0 07 0 07 0 06 0 06 0 06 0 02 0 02 0 02 Result 2 3 324 3 294 3 286 Unit 2 pg mL pg mL pg
11. A and RNA Touch Options for the parameter setup Nucleic Acid Analysis UV VIS X ds DNA More displays further options in the Options screen 1 8 3 0 E View Conc Abs lets you toggle between the screen that pg mL EES displays the result data concentration and the screen that SDN 1 0000 shows the raw data absorbance values 1 837 50 000 au m o Dilution X lets vou enter a dilution correction factor The displayed results are corrected for dilution using the following equation 09 FEB 2005 17 19 25 j corr Conc x Dilution A lets you modify the default wavelengths and toggle background correction on and off When background correction is on you can modify the default background wavelength Timer Icon sets the timer Using the timer does not affect the readings that the instrument is taking The times are not stored with sample readings Mur 8 50 11 00 4 11177 Pis 0140015 e a note gadget Bmorrison on m I Morrison Prot a ica Microsoft Pow la http users e mI 7 ep Spectrophotometry DU 730 Print Send data to USB devices Sec 14 3 Address http users edutdepartrnentsifybiol Spectrophotormeter DLIZOU Manual pdf Links gja Customize Links ejr Free Hotmail il windows ES windows Windows Media i STLC FY BioTechnology Home Page
12. CW to 9am position to remove CW to 12pm to lock in place STLCC CPLS Morrison 6 12 2015 Page 35 Spectrophotometer DU 530 Setup Cleaning Lens 1 Open the Cell and loosen the j screw on the bottom 2 Remove the cuvette fitting and clean the lens on both sides with a tissue 3 Replace the fitting and tighten the screw Clean bubble lens STLCC CPLS Morrison 6 12 2015 each side with tissue only do Not use acetone or solvents Page 36 Spectrophotometer DU 530 Setup cuvette Handling A 1 When handling the cuvettes for the Blank Buffer and the Sample avoid contact with the clear sides of the cuvette Handle it only by the opaque sides 2 Label the white stopper on the cuvettes B blank buffer Sz sample 3 Wipe the clear sides of the cuvette with lab tissue before inserting it into the holder opaque sides toward the front and rear 4 Clean cuvettes after use with soap and water rinse with deionized water before use STLCC CPLS Morrison 6 12 2015 Page 37 Spectrophotometer DU 530 Membrane Keyboard Description 32 Keyboard Description 1 Soft Keys 6 Decimal Point and Instrument Setup 2 Exit 7 Numeric Pad 3 Scroll Keys 8 Printing or Download Display 4 Enter Data 9 Data Recall 5 Clear Entry 10 Data Storage Page 38 STLCC CPLS Morrison 6 12 2015 Spectrophotometer DU 530 Selecting Options or Setting Values Select the Soft Key arrow buttons below and
13. Pedestal Before and After Each Test STLCC CPLS Morrison 6 12 2015 Page 57 Spectrophotometry NanoDrop Save Report Protocol 1 After all samples have been run on the Main Screen select Show Reports 2 Select the Reports Tab at the top of the screen 3 Select the Save Report button 4 Select Export Report Table Only button to save the current report data to a neutral file format txt 5 Save the Report txt file to your flashdisk or another file to email it to another computer for further analysis or printing STLCC CPLS Morrison 6 12 2015 Page 58 STLCC CPLS Morrison 6 12 2015 Spectrophotometry NanoDrop Import Saved Report txt file to Excel On a computer with MS Office and printer access install the flashdisk or download the exported file from Email Open the Excel application and select DATA in the top toolbar Select Import External Data from the drop down menu Select Import Data Locate the report txt file on the flashdisk or other media and open the file Follow the Excel file import procedures select the Delimited option then Next and Finish to import the file Review the Report data in the Excel spreadsheet adjust column widths as needed Save the Excel file to the flashdisk or other location for processing and printing Page 59 Spectrophotometry NanoDrop Troubleshooting Ratio Error Messages 1 lf you get a message similar to Ratio between long and short path out of tolerance use t
14. Setup Mode to activate the Carousel Options and set up the number of cell positions used and the orientation of blanks and samples Position numbers 1 7 stamped on cuvette slot Alignment tab yellow dot on carousel must be aligned with similar tab dot on compartment below Carousel Holder Installation 1 Open the cell compartment 2 Place the Carousel Holder on the rotatable attachment on the bottom of the cell compartment so that the marking faces upward 3 Take care to position the holder exactly The Alignment Tab yellow on the holder and the rotatable attachment must line up exactly 4 Turn the holder slightly to the left or right until the guide key locks into position This establishes contact with the instrument For instrument setup options and procedures see Carousel and Module Options on the next slide STLCC CPLS Morrison 6 12 2015 Page 12 Spectrophotometry DU730 Carousel Setur Instrument stup If your 5trument includes a Carousel Holder the Instrument Operator ID Sample ID Sety menu includes Carousel Options You can activate the rum EU Arousel Holder and modify the carousel parameters using in this Date amp 2 0 Display amp menu Time Cbd Sound f us died CP USB P 1 Activate the Carousel Holder M a Touch Carousel Options in the Instrument Setup menu assw ord 1 Touch Carousel Off to change its display to Carousel On 22 Touch OK to confirm your selection Se
15. UNITY COLLEGE PITTSBURCH PA 15230 RICHARD NORRIS REF 1305796 BRDG PARK AT DDPSC PLANT amp LIFE SCI 1005 N WARSON RD RM 122 ST LOUIS MO 63132 Bill of Lading No 850525727413 Spectrophotometer Date Shipped 03 18 2013 Carrier Fed Ex Second day Nanod rop Cal Kit Incoterms 6 Origin Collect Total Weight 1 000 LB Order ng Number of packages 00001 LEASE SHIP FEDEX ACCT 260751441 tem Material Description Serial No 010 CHEM CF 1 CF 1 CALIBRATION FLUID Order No 18040547 1 Page 65 Spectrophotometer Nanodrop R126B Latest Calibration Test Results Customer Guidance 3 21 2013 11 16 AM Blank Calibration check is complete Click Reset to begin again or Exit to return to the Main Menu i Room 268 Instructions Lom Target Abs 350 nm 1 0 mm path 0 737 2 2 Measurement nm 350 amp Replicates 10 V a Correctionnm 600 0 90 10mm Path 0 2 mm Path NAN ODRO Target Abs 7 073 0147 0 80 Current Abs 0 756 04147 CALIBAHTI on TEST Ave Abs 0 757 0 147 p its 96 Error 2 7 05 4 j Std Dev 0 006 0 001 B 0 60 3 2 i 52 HD 0 50 1 mm Path S 0 2 mm Path E 0 40 v Measured Absorbance vs Target z 0 30 amp 0 20 N G 0 10 al at M 0 107 i I I U 220 250 300 350 400 450 500 550 600 650 700 750 Wavelength nm 0 0507 i i i i i 0 1 2 3 4 5 6 7 8 9 10 3 3 BC184 0 21 80 16 Measurement w c
16. alculations include molecular weight nucleotide length extinction coefficient concentration and melting temperature Dye Incorporation The Dye Incorporation mode works for single and duo color methods spotted array This works for any dye that gives similar fluorescence wavelength to Cy3 550nm or Cy5 650 nm DNA Protein Tools The DNA Protein Tools mode does not take measurements The mode provides a number of calculations conversations and tables to aid you in common daily tasks System Checks The System Checks mode lets you check the performance of the instrument photometric accuracy photometric noise stray light wavelength accuracy lamp history and printer check Hecall Data The Recall Data mode lets you store recall send and erase data from the Datalog Instrument Setup The Instrument Setup mode allows you to configure the instrument and the module based on your specific requirements DW 8 50 11 00 Af D AUD 1 J Pis PLS Morrison e ter2g15 2 Emorrison on Fcl TJ Morrison Prokacols a note gadget txt m ic Microsoft PowerPn n Spectrophotom ED 11 29 AM Spectrophotometry DU730 dsDNA ssDNA RNA Options cont stlcc edufdepartmentsjfvbiafSpectrophotometer SO Links Customize Links Free Hotmail Windows Yo Windows Marketplace Windows Media ii STLC FY BioTechnology Home Page U St ee m dsDNA ssDN
17. aliquots of the CF 1 Calibration Fluid measure 10 replicates After the 10 measurement the calibration check results will be displayed on screen in the Customer Guidance text box f If the instrument does not the calibration check using 1ul samples immediately repeat the procedure again step 5 using 2ul samples To print a copy of the resulisioruour records click tha Sprint Corea es ofthe final results is automatically archived c If recalibration is required infofinanodrop com Spectrophotometry Nanodrop Calibration NOTE The CF 1 Calibration Fluid is supplied in a single use vial The CF 1 must be used within one hour of opening the vial Exposure to the environment or transferring of the fluid to another container may cause a significant change in Page 62 Spectrophotometer Nanodrop Calibration Kit Step 1 2 A vial of CF 1 aqueous potassium dichromate K Cr O solution is required to run the calibration check procedure for the Thermo Scientific NanoDrop 2000 2000c and NanoDrop 1000 Spectrophotometers Procedure 1 Ensure the measurement pedestals are clean and that a 1ul water sample beads up on the lower pedestal 2 Remove any lint build up from around the instrument solenoid by following the instructions on the back page of this docu ment Applies to NanoDrop 1000 only 3 Open the Calibration Check Software and follow the prompts in the Customer Guidance text box of the software 4 E
18. ank and 1 5 sample positions e Deletes the last character entered P 009 positions when used in Auto 3 mode STLCC CPLS Morrison 6 12 2015 Page 23 Spectrophotometer Genesvs Cell Holders Cuvettes 6 Position Cell Holder dp ia mm im n msn mma PI E lt lt SS Note If the Cell Positioner method option is set to Auto 6 whenever you press Run Test to start a measurement the instrument attempts to initialize the cell positioner If a single cell holder is installed the message Error Single Cell Holder found Use Single Cell Holder appears Press Accept Change to continue the measurement with a single cell or install the 6 cell changer and press Cancel Change See the parts list for a more detailed list of available accessories Selecting and Positioning Cuvettes The compatible wavelength range for different types af cells depends On the material used Cell Type Wavelength Optical Glass 360 nm to gt 1100 nm Borosilicate Cilass 330 nm to 1100 nm Disposable Quartz 190 nm to gt 1100 nm Polystyrene gt 340 nm Methacrylate gt 300 nm Acrylic gt 280 nm STLCC CPLS Morrison 6 12 2015 alli Pages opectrophotometer Genesys Measurement Options Auto 6 Auto 3 Measure one blank and up to five samples without changing cuvettes in the cell changer The instrument automatically measures the blank and advances the cell changer to measure the re
19. ce or Sample Wavelength or to adjust Set Ref Used to cause a read of the blank for reference F TECHNICAL SPECIFICATIONS Mode Button Wavelength range 330 800 nm Use to select Bandwidth 7 nm Abs Conc Absorbance range 0 300 to 2 50 Trans Factor opectrophometer VIS General Operating Protocol SILCC CPLS Morrison 6 12 2015 A Push the On Off toggle button on the rear to turn on the machine It will then display CALC and cycle from 1 2 3 then to indicate system calibration is complete 1 Press or wavelength buttons to set the desired wavelength Press to change CONC or FACTOR 2 Press MODE button to cycle display to ABS TRANS CONC or FACTOR Set mode to ABS is recommended 3 Fill a BLANK cuvette at least full with distilled water and wipe outside of cuvette with a tissue to ensure it is clean and dry 4 Insert the cuvette completely into the compartment orienting the line on the cuvette with the mark on the sample compartment 5 Close the sample compartment cover and press SET REF to zero instrument ABS will be 0 0 after this operation Trans 2100 Concz 001 6 Remove the cuvette and fill a cuvette at least full with SAMPLE and wipe outside of cuvette with a tissue to ensure it is clean and dry T Insert the cuvette completely into the compartment orienting the line on the cuvette with the mark on the compartment 8 Close
20. concentration in ug ml additionally in pmol ul for oligonucleotides eMolar extinction coefficient and molecular weight of oligonucleotides Link to Bio Rad SmartSpec Plus User Manual pdf STLCC CPLS Morrison 6 12 2015 Page 69 Spectrophotometer SmartSpec Quick Start Guide pg 1 1 Power up the instrument It will so through system status check and display an error message if there are any problems a Press one ofthe Assay buttons A DNA RHNA 1 Choose type of nucleic acid b dsDNA ssDNA or RNA accept or modify the conversion factor DHA oligo or RNA oligo input molar extinction coefficient and molecular weight otherwase choose method for SmartSpec Plus to estimate these values 11 Choose whether to subtract backeround and 1 so specify backeround wavelenzth B Protein i Choose type of assay c Teo oF Bradford Measure absorbance at 595 nm Lowry Measure absorbance at 750 nm BCA Measure absorbance at 562 nm UV Measure absorbance at 200 280 and 320 nm Other User specifies the wavelength to read i Choose standard curve option b STLCC CPLS Morrison 6 12 2015 Create anew standard curve Recall a standard curve from memory Ho standard curve Smartopec Plus will not be able to convert absorbance to concentration Page 70 Spectrophotometer SmartSpec Quick Start Guide pg 3 SILCC CPLS Morrison 6 1 U eran i and lower limits of scan 200 nm
21. device to a USB port on the Host CPU 6 Also connect a Flashdisk to the Laptop if you want to transport report data for further analysis and printing STLCC CPLS Morrison 6 12 2015 Page 54 opectrophotometry NanoDrop Software Main Menu ETT 3 2 0 a Link to Nanodrop User Manual pdf STLCC CPLS Morrison 6 12 2015 Page 55 Spectrophotometry NanoDrop Test Protocol 1 Double click the NanoDrop Software icon from the Host Laptop desktop 2 Follow the on screen procedures page 4 to select the type of Sample being tested 3 Open the Nanodrop switch blade reader arm and wipe the bottom and arm pedestal surfaces clean of any solution or lint debris Close the arm to the down position 4 The System will perform initialization procedures and then ask for a BLANK test 5 Pipette 2 uL of SAMPLE on the reader and inspect to see that a good bead of the sample is formed If the Sample is not beaded wipe clean and pipette again 6 Select the MEASURE button on the screen and the test will run automatically 7 Examine the plot and concentration data displayed on the Screen Write down any important data and proceed 8 Continue with further samples as required STLCC CPLS Morrison 6 12 2015 Page 56 Spectrophometry NanoDrop Technique Illustrations Dispense 2uL to Lower Pedestal Observe good bead Before proceeding Bead Supported by Surface Tension before Measure command Wiping Lower
22. ecord at least 3 readings and check with concentration on DNA source t 1 TINER 50 ig ml x d VO OQ C NO ds DNA A260 NM x Page 43 Spectrophotometry DU 530 Lamps and other Maintenance Issues Access to Lamps and Mirrors for replacement and cleaning 1 Push in both sides of the small door in the upper part of the back panel 2 Swing up and remove the door 3 Loosen ccw the two screws holding the lamp cover plate and remove the plate 4 Carefully clean circular mirror it is free to rotate not fixed 5 DO NOT touch the lamps with bare fingers Use a cloth or lab wipe Remove Load cuvette or carousel holding cell CCW to 9am position to remove CW to 12pm to lock in place STLCC CPLS Morrison 6 12 2015 Page 44 Spectrophotometry DU 530 Lab Assays 1 and 4 Sample wavelength nm ug mL pg mL ug mL Average Reading Reading Reading Ix TNE buffer 325 The Assay 4 Warburg Christian results are entered in this area of your lab worksheet Unless the Dilution Factor DNA Sample 260 was set on the Spectrophotometer this must be calculated here DNA Sample 2 260 DNA Sample 3 260 The Assay 1 260 280 ratio results are DNA Sample 1 260 280 DNA Sample 2 260 280 DNA Sample 3 260 280 STLCC CPLS Morrison 6 12 2015 Page 45 Spectrophotometer DU 530 Verify Quality and Quantity wit
23. engths are within calibration specifications A vial of CF 1 is required to run the calibration check procedure CF 1 is an aqueous potassium dichromate K Cr 0 solution for use in confirming calibration of NanoDrop Spectrophotometers It is roughly ten times more concentrated than other commercially available Kz rO solutions used to confirm calibration of standard spectrophotometers and is available through NanoDrop technologies or your local distributor Procedure Ensure the measurement pedestals are clean and that a 1ul water sample beads up on the lower pedestal 1 Open the ND 1000 Calibration Check Software and follow the prompts in the Customer Guidance text box of the 2 Enter the Target Absorbance found on the CF 1 vial as directed in the image below Typically the target absorbance is 0 734 the actual value will depend on the lot of CF 1 concentration Von THH m 3 im 1 244 a M je n a 1 LS a rmm am cxi amp unes ener em rete 3 Add 1ul of deionized water and select Blank 4 Before opening the ampoule of CF 1 Calibration Fluid shake vigorously to ensure solution is thoroughly mixed Ensure all solution is collected in the bottom portion of the ampoule gt Carefully break the neck of the ampoule to open the CF 1 Calibration Fluid 6 Follow the on screen prompts in the Customer Guidance text box Using individual 1ul
24. ge to sensitive samples as the lamp only flashes when data is being acquired e Save money with long lifetime xenon flash lamp guaranteed for 3 years 2 Basic ATC select Lamp produces almost no heat so sample compartment TEST button temperature remains stable 3 To Change e See more at parameters select http www thermoscientific com content tfs en product genesy Utility button 10s uv vis spectrophotometer htmlzzsthash MyL Kflh Y dpuf Link to Thermo Sci Genesys 10S UV Vis Users Guide pdf STLCC CPLS Morrison 6 0 Page 21 Spectrophotometer Themo Sci Genesys 10S UV Vis BRDG GENESYS 10S Series Specifications GENESYS 108 UV Vis GENESYS 105 Vis Spectral Bandwidth 1 8 nm 5 0 nm Light Source Typical Lifetime Xenon Flash Lamp 5 years 3 years quaranteed Tungsten Halogen Lamp 1000 hrs 190 1100 325 1100 nm x 1 0 nm 05 nm 11 000 nm min Up ta 3600 nm min 0 1 0 2 0 5 1 0 2 0 5 0 nm 1 0 2 0 3 0 5 0 nm Up to 3 5 A at 260 nm Up to 3 0 4 at 340 nm 0 1 1 5 125 7 9999 C 11 3 A 0 3 125 8T 9999 0 005 A at 1 04 0 5 or 0 005 A 0 010 A Kr whichever ls greater up to 2 A Noise 000025 at 0 0 A 001 A at 0 0 lt 0 00050 at 1 0 A 002 A at 2 0 D 00080 at 2 0 A Peak ta peak at 340 nm AMS at 260 nm Drift 0 0005 0 002 A hr after warm up Stray Light lt O0 08 T at 220 340 nm Nal NaN lt 1 7T at 340 and 400 nm lt 1 0 198 200 nm K Displa
25. h the UV 10 10 nm 1 nm 10 nm 10 nm Gamma rays X rays UV Infrared Microwaves Radio waves Visible light 380 nm 450 nm 500 nm 550 nm 600 nm 650 nm 700 nm 750 nm Wavelength increases 3 a Energy increases 1999 Addison Wesley Longman Inc 1m 109 nm 103 m DNA absorbs electromagnetic energy at 260 nm Protein absorbs at 280 nm 260 280 ratio indicates purity Highly purified DNA has an Az60 A280 Of 1 8 1 8 2 0 desired 1 8 indicates substantial protein or phenol contamination Pure protein ratio 0 6 otally pure RNA has an Az60 A250 Of 2 0 HNAin the DNA is detected as DNA gt inflated DNA RNA can be degraded either chemically or enzymatically prior to measurement From Bio219 Lab 4 Estimating quantity using the UV Spec 90 ug ml DNA has an absorbance of 1 0 at 260 nm The concentration of any sample of double stranded DNA can therefore be calculate using the following formula ds DNA gt nm x 50 ug ml x d f Example 10 pl of ds DNA was added to 1 ml of water in a cuvette The Ass was 0 021 What is the concentration in ug ml of the original undiluted sample Answer The dilution is 10 ul 1000 ul 1 100 0 021 x 50 ug ml x 100 105 ng ml opectrophotometer VIS Novaspec ll GE Amershame Pharmacia Biotech On Off Toggle on rear panel N Mode Settings Abs Trans Conc Factor Door Open to insert Blank Referen
26. his procedure 2 Residue has likely collected on the pedestals lower and or arm from previous use 3 LN gt 2uL of dH20 on the pedestal close the arm press down lightly on the arm or 2 min 4 Wipe the pedestals and repeat the water soak procedure o Wipe the pedestals gt 30 times lower and arm with a clean lab wipe 6 Place a 2uL bead of dH2O on the lower pedestal and look for a good bead of water T Retest with your sample if the problem persist the pedestals need to be reconditioned STLCC CPLS Morrison 6 12 2015 Page 60 Spectrophotometry NanoDrop Troubleshooting Reconditioning the Pedestals 1 Locate the PR 1 Reconditioning Kit in the Nanodrop drawer 2 Usethe supplied applicator apply a pin size drop of the compound on the lower and arm pedestals 3 Spread the amount around with the applicator and let it dry 30 seconds 4 Using a folded clean lab wipe remove the PR 1 compound by rubbing vigorously the lower and arm pedestals Be careful not to put too much pressure on the arm while rubbing 5 The appearance of a black residue on the wipe is normal use another wipe and continue 6 Testthe effectiveness of the reconditioning by pipetting a 2 uL sample of dH20 and look for a good bead T Resume testing with samples STLCC CPLS Morrison 6 12 2015 Page 61 The Calibration Check is found within the Utilities and Diagnostics module and is accessed through the Main Menu It is used to confirm that both pathl
27. indows Marketplace Windows Media ii STLC FY BioTechnology Home Page You can send scan data to a printer or a memory device using the available USB ports Since the system automatically senses connected devices it sends the data to all available devices To send scan data directly from the Wavelength Scan mode 1 Touch Options 2 Touch More 3 Touch Send Data USB Port icon The instrument e ed NES immediately sends to the connected devices the data of all Setup scans that currently reside in memory Return To send sean data from the Recall Data screen Wavelength Scan 27 OCT 04 17 55 55 1 Highlight the line with the desired scan scan 1 423 451 nm 4 5 0 nm 29 OCT 04 16 54 00 2 Touch Optlons Scan 2 423 453 nm 5 0 nm 10 MOV D4 15 01 52 Tawh Sen a TIG inam The de cud cH ME EE 3 Touch Send Data USB Port icon The instrument MMC PM immediately sends to the connected devices the data of the Scand 423 453 nm A 5 0 nm selected scan NOTE You may repeat this procedure for the remaining scan data you want to send to the target location s jore 70 Page e1 Ke IP Bke EAA LSE 0140015 e a note gadget t 2 Bmorrisan on zd CI Morrison Prot fe Microsoft Pow It http users mI 7 3 26 Spectrophotometry DU 730 Excel Import CSV File ex Parameter 260 280 Ratio 260 280 Ratio 260 280 Ratio 260 280 Ratio 260 280 Ratio 260
28. maining samples Measure one blank and two samples without changing cuvettes in the cell changer The instrument automatically measures the blank and advances the cell changer to measure the samples in positions 2 and 4 Single Cell Holder Manual 6 Place the blank in the cell holder measure it place a sample in the cell holder and then measure your sample This process is completely manual The cell position buttons do not function when you select Single Cell Holder Note You can cause the 6 Cell Changer to function as a single cell holder and measure only one cell by selecting this option However we recommend that the single cell holder be used for accessories requiring a high degree of positioning repeatability such as the nanoCell accessory small aperture microcells the coupling module of fiber optics probes and flowcells used with a sipper system Measure a blank and up to five samples without changing cuvettes in the cell changer using the cell position buttons to advance the cell changer to the appropriate position for the next measurement Place the blank in the blank position and your samples in the other cell positions Regardless of where the cell holder is positioned when you press Measure Blank the cell holder automatically goes to the blank position and measures the blank However you can use the cell position buttons to select a different position for the measurement Note When you have the Cell Changer installed
29. nter the Target Absorbance found on the CF 1 vial as directed in the images below 5 Add lul of deionized water and select Blank 6 Before opening the ampoule of CF 1 Calibration Fluid shake vigorously to ensure solution is thoroughly mixed Ensure all solution is collected in the bottom portion of the ampoule 7 Carefully break the neck of the ampoule to open the CF 1 Calibration Fluid 8 Follow the on screen prompts in the Customer Guidance text box Using individual 1ul samples of the CF 1 Calibration Check Fluid measure 10 replicates 9 After the 10 measurement the calibration check results will be displayed on screen in the Customer Guidance text box If the instrument does not pass the calibration check using 1ul samples immediately rerun the procedure step 8 using 2ul samples 10 The NanoDrop M 2000 2000c software will archive the results in the autosave file at C Documents and Settings All Us ers Documents Thermo NanoDrop2000 AutoSave CalibCheck 11 The NanoDrop 1000 software will archive the results will in the NanoDrop data folder at C NanoDrop Data Calib check 12 Ifusing the NanoDrop 1000 calibration check specific software downloaded from our website the results are not automati cally archived and must be manually saved to retain a record 13 If recalibration is required contact us 302 479 7707 or by email at nanodrop a thermotisher Note The CF 1 Calibration Fluid is supplied in a single
30. of DNA concentration he calculations are performed by the spec using A 260 0 nm and constants calculated by Warburg and Christian A reading at A 320 is also automatically performed and any correction for background absorbance by the buffer and cuvette are included in the calculation NUCLEIC ACID Warburg Chr ist ian AG I QN 32 FEB Note At the end of the WC run this will read 320 the last wavelength run for the correction factors The 260 and 280 runs have already been made stored Protein and used in the results Abs tk tAbsotks Dil PathLen Nucleic Abs tke tAbs tk4 Dil zPathLen REQUIRED STIMER 60 00 Measurement control BLANK OPTIONS Note Press the soft key below DIL X 1 0000 Type in the dilution factor of the DNA in the 1X TNE buffer ex 11L DNA to 2 mL 1X TNE type in 2000 Press ENTER STLCC CPLS Morrison 6 12 2015 STLCC CPLS Morrison 6 12 2015 Spectrophotometer DU 530 Lambda DNA Test Example 1 Setup system per this protocol Load 2mL of 1xTNE buffer in cuvettes for Blank and the Sample Add 1 uL of Lambda DNA to Sample Select ds DNA for type of run Verify and or set Conc X z 50 00 Set DIL X 2000 per steps 2 3 above Before reading the BLANK wait for the UV to stop blinking in the UV VIS box in the upper right of the screen Run BLANK then you are ready for samples Run DNA sample r
31. on automatically loads this test and prepares for immediate measurement It you select more than one test as SmartStart tests the instrument when powered on automatically displays a menu containing only those tests Note You can always access the default main menu by pressing Test set up a single test SmartStart unselect a test I Press Utility to display the Utility screen 1 Press Utility 2 Highlight Stored Tests Directory and press Enter 3 Highlight the test to be removed and press Unselect Test The Main Menu will be displayed upon power up set up a multiple test SmartStart 1 Follow steps 1 through 3 in the procedure above for setting up a single test SmartStart 2 Select the desired tests An arrow sign gt indicates the tests selected for the SmartStart menu Press Esc to return to the Utility screen or power down the instrument Press T or to select parameter Note To remove tests from the SmartStart menu see the procedure above for unselecting a test 2 Highlight Stored Tests Directory and press Enter 3 Highlight the appropriate test and press Select Test An arrow sign gt indicates the test has been selected for the SmartStart menu Page 33 cn Press Esc to return to the Utility screen or power down the instrument Spectrophotometer DU 530 General Instructions owing open the flip top screen Turn on the device lower left rear cradle switch Observe Basic System
32. orbance pathlength resulting in erroneous measurements I Lay the instrument on its side with the source fiber black fiber optic cable facing up and open the sampling mechanism Refer to the image on the right 2 Using a paperclip or a small screwdriver manually depress the solenoid plunger and spray com pressed air down the solenoid plunger hole Be sure to keep the can of compressed air upright so as not to spray the propellant into the instrument For Technical Support contact us at 302 479 7707 or nanodrop Q thermofisher com Rev 2 09 STLCC CPLS Morrison 6 12 2015 Page 68 Spectrophotometer SmartSpec Plus Bio Rad s NW Pi v us e co m The UV visible SmartSpec Plus spectrophotomer has a working wavelength range of 200 800 nm It is the perfect tool for routine applications such as eQuantitation of DNA RNA and oligonucleotides eQuantitation of proteins via the Bradford Lowry and BCA assay methods eMonitoring bacterial culture growth eSimple kinetic assays eWavelength scans with peak detection A simple menu driven interface simplifies assays and provides answers to common sample computations at the touch of a button Conversion factors can be stored and modified The omartSpec Plus spectrophotometer is capable of performing calculations and providing results such as e Asso Asso ratio for nucleic acid purity eQuantitation that takes dilution factors into account eSample
33. r Carousel Holder Well Performance Specifications DU Series 700 UV Vis Scanning Spectrophotometer Operating Mode Absorbance and Transmittance T Source Lamp Deuterium UV and Tungsten visible Wavelength Range 190 to 1100 nm Wavelength Accuracy 1 nm from 200 to 900 nm Wavelength Calibration Automatic ocanning Speed Depending on selected resolution 100 4500 nm min Wavelength Resolution Selectable Interval 0 1 0 2 0 5 1 0 2 0 and 5 0 nm Spectral Bandwidth lt 3 nm Photometric Readout 0 3 to 3 0 A or 0 1 to 100 T Photometric Accuracy 0 005 A at 0 0 to 0 5 196 at 0 5 to 2 0 Photometric Linearity 0 596 at 2 0 lt 1 at gt 2 0 Stray Light gt 3 3 A or lt 0 05 T with Kl solution at 220 nm System Configuration SN 1283669 Ver 1 05 40 Link to Beckman DU 700 Series Users Guide pdf STLCC CPLS Morrison 6 12 2015 Page 3 Pa 1 oe x 10 11 SILCC CPLS Morrison 6 12 2015 Spectrophotometer DU 730 General Instructions Leave the sliding lid closed until you are ready to insert cuvette for a reading Turn on the device rear upper left side toggle switch Install a Flashdisk in an open USB port if printed results are needed Observe Basic System Checks pass or fail on the display screen Prepare Blank and Sample be careful not to touch the clear sides of the cuvettes Label the stopper for each S sample or B blank On the Main Menu screen select Instrument Setup
34. s Select Start or Select by 1 260 280 Ratio S Number to scroll to the desired Single Ratio Double Ratio amp Concentration type of analysis sample ds DNA sS DIA e 6 7 Oligo DNA Long 8 Oligo DNAShot em a RNA Long N j gt t A MP Hort Bui Nucleic Acid Analysis More view Conc i Dilution X m m Z o 0 2100 Select Options to enter items 1 0000 such as Dilution Factors or to see other options select More 19 SEP 2009 14 12 STLCC CPLS Morrison 6 12 2015 Page 9 opectrophotometry DU730 Options Dilution Keyboard Dilution Correction Use numeric keyboard to enter 1 0000 _ factors then select OK when done STLCC CPLS Morrison 6 12 2015 Page 10 opectrophotometry DU730 Lamp Warm up Messages Nucleic Acid Analysis is DNA After selecting Blank or Sample a UV Lamp Warm up message may appear Wait until the Lamp Warmup UV VIS message stops blinking and the menu shows Blank before zsoo Azeoo trying to proceed 93 SEP 2009 14 13 03 im i Nucieic Acid Analysis 3 SEP 2009 14 125 STLCC CPLS Morrison 6 12 2015 Page 11 otometry DU730 Carousel Cell Holder The Carousel Cell Holder Part no A23620 allows you to load up to seven cuvettes of solution into the instrument for analysis at one time The cuvettes can be various combinations of blanks and samples Use the
35. t the carousel options In str ee a i Carousel options f or oj la a Touch Carousel Options and touch Blank 1 7 Read 1 7 Select the carousel option of your choice C Blanki Read 2 7 AO C Blank 1 3 5 Read 2 4 6 For example the first option is Blank 1 Read 1 7 which Cells means that the blank cuvette will be read in position 1 r ab while samples must be placed in the cell positions 1 7 With this option you must remove the blank cuvette Cancel TS OK after blanking Use the arrow keys to specify the number of cell positions you want to use Touch OK to confirm and return to the Instrument Setup menu NOTE As an alternative you can access the Carousel Options screen directly from the measurement mode by touching the Carousel Icon on the bottom right of the screen The Carousel Options screen appears and you can quickly make the necessary changes Pis CF Lp 12220152 Emorrison an Fcl Morrison Protocals a note gadget txt la http users stlc fe Microsoft PowerPo E 7 5 34 AM Spectrophotometry DU730 Main Menu Options pg 1 2 TEE ea gt Links Customize Links Free Hotmail Windows Lo Windows Marketplace Windows Media i STLC FY BioTechnology Home Page 2 E Table 3 1 Main Menu Soft Key Options T Soft Key Operation Mode User Programs The User Program mode lets you recall saved programs Fixed Waveleng
36. th Measure Blank Set Wavelength Press Set ntn or any number key to set the wavelength Absorbance 17 25 1 pr 1 O 219A 2 Enter the wavelength for taking measurements and press Set nm again Measure Blank To measure a blank 1 Place the blank in the cell holder If a 6 Position Cell Changer is installed place the blank in the B position loenter an absorbance or transmittance value for the blank press a number key and enter the desired value in the Entry field 3 Press Measure Blank STLCC CPLS Morrison 6 12 2 Spectrophotometer Genesys Measuring Samples If a 6 Position Cell Changer is installed place the samples in the cell positions and press the corresponding cell position button to move the cell holder to the measuring position The absorbance ABS or percent transmittance measurement appears on the display f a Single Cell Holder is installed remove the blank and place the sample in the cell holder Ihe absorbance or ransmittance measurement appears on the display STLCC CPLS Morrison 6 12 2015 Page 32 Spectrophotometer Genesys SmartStart Setup The SmartStart feature lets you customize the spectrophotometer by placing the most frequently used test methods on the first menu Right after the instrument initializes a simple menu containing only the SmartStart tests appears If you select one test as a SmartStart test the instrument when powered
37. th The Fixed Wavelength mode collects data at up to 4 wavelengths You can display the data in absorbance transmittance or concentration The concentration 15 calculated by a user defined factor or formula Wavelength Scan The Scan Wavelength mode shows how a sample absorbs light over a range of wavelengths You can use this feature to optimize instrument sensitivity with a given chemistry The screen plots the scan Kinetics Time The Kinetics Time Measurement mode records absorbance or transmittance at a single wavelength over a specified time period Single Component Analysis SCA The SCA mode lets you prepare a standard curve for analyzing samples that contain one component Protein Assay Analysis The Protein Assay mode provides a simple procedure to calculate the amount of protein in a sample It contains pre selected parameters for seven common protein assay methods TM Mas DU Series 700 User s Guide _ hl oA AMA A A 8 50x11 00im Af T el Done e Unknown Zone Pis Fb TZ 14 015 e a note gadoet t Bmorrisan an T I Morrison Prot a ica Microsoft Paw It http users e 7 e Spectrophotometry DU730 Main Menu Options pg 2 2 lz Installation es Main Menu Table 3 1 Main Menu Soft Key Options is Nucleic Acid Analysis The Nucleic Acid mode provides ten methods for DNA RNA and oligonuceotide analyses which calculates nucleic acid absorbance ratios and concentrations Other c
38. the compartment cover and a new reading for the SAMPLE will be displayed Read and record the absorbance values 9 Every time the wavelength is changed the instrument must be re zeroed using the SET REF button Page 50 Spectrophotometer VIS only Novaspec lll or Plus Planning info only NOVASPEC III 80 2118 00 VWR CAT 97058 518 Each 2 335 00 NOVASPEC PLUS 80 2117 50 VWR CAT 97058 500 Each 2 735 00 NOVASPEC PLUS Supplier GE Healthcare Novaspec Plus Visible Spectrophotometer Wavelength range nm 330 830 nm Absorbance range A 0 3 2 500 Wavelength accuracy 2 nm Optical system Single beam monochromator STLCC CPLS Morrison 6 12 2015 Page 51 Spectrophotometry Nanodrop STLCC CPLS Morrison 6 12 2015 Page 52 Spectrophotometry NanoDrop Instrument Setup Illustration Power Adapter White USB Standard A B cord STLCC CPLS Morrison 6 12 2015 Page 53 Spectrophotometry NanoDrop Hardware Setup Protocol 1 Use the bench CPU Host or checkout 3 or 4 or the Staff laptop as they are the only ones presently loaded with the Nanodrop Application Software 2 Locate a Nanodrop device from the bottom drawer cabinet under the CEQ 8000 Genetic Analyzer device 3 Retrieve a Nanodrop power adapter black and USB white cable from the same area 4 Connect the power adapter to a 110 volt outlet and then to the round port on the Nanodrop device 5 Connect the white USB cord from the Nanodrop
39. the instrument always considers the material in the B position as a blank This means that even after measuring your blank the first time you can place samples only in positions 1 through 5 Spectrophotometer Genesys Basic ATC Mode Basic mode puts the instrument into an instant measurement mode he user simply walks up to the instrument inserts a sample and measures it Depending on whether the mode is set to Absorbance A ransmittance 1 or Concentration the result appears along with the type of measurement the date and time the wavelength and the cell position used for the measurement toggle between Absorbance ransmittance and Concentration press Change Mode You can toggle modes whenever you see Change Mode Absorbance 16 03 31JanOZ When Basic ATC is set to Absorbance or Transmittance these capabilities are provided s Setting the Wavelength Measuring a Blank Measuring Samples STLCC CPLS Morrison 6 12 2015 Page 26 opectrophotometer Genesys Select Basic ATC button STLCC CPLS Morrison 6 12 2015 Spectrophotometer Genesys Basic ATC Absorbance Page 28 STLCC CPLS Morrison 6 12 2015 Spectrophotometer Genesys Basic ATC Transmittance Page 29 STLCC CPLS Morrison 6 12 2015 opectrophotometer Genesys Basic ATC Concentration Page 30 STLCC CPLS Morrison 6 12 2015 Spectrophotometer Genesys Set Waveleng
40. use vial The CF 1 must be used within one hour of opening the vial Exposure to the environment or transferring of the fluid to another container may cause a significant concentration change STLCC CPLS Morrison 6 12 2015 Page 63 Spectrophotometer Nanodrop Calibration Kit Step 2 2 Cousin a API 446 Mam ure em 161 ro pila aer sdb CE hallow Ihre queete hor vagum action 7 AA Exit Tenet Abe 64 3 0 rea 1 0 m pat Mens m n ren 0 Comochon em Hb o I b we Pl rene Alm 147 m E Net E ras 120 140 A Jl nen uli i 1 s Meatured Absmibeatco ve Tosyet 1v T ip t i a E 7 2 Lio LAF LESS i dle n i T e TE D zz m nei TY Ww orta illic i P m m 1 13329 3259 panim d NanoDrop 1000 Calibration Check Screen STLCC CPLS Morrison 6 12 2015 Page 64 Delivery Murnber 830 1284 Customer Purchase Order Number Data DR3680388 03 13 2013 Sales Order Number Date 180405471 03 13 2013 Thermo PACKING LIST ELECTRON CORPORATION Thermo Electron Scientiie Insthumanis LLU Sold Ta Customer 1145990 5225 Verona Road Madison WI 53711 608 275 6100 Page 1 1 Bill To 1145990 Wa our Ship To No 1145990 re eoi v ST LOUIS COMM
41. vette into SmartSpec Plus and press Read Sample 7 After last sample press left arrow key to exit assay 8 Forprotein assays save the standard curve Gf you made a new one and print the full report STLCC CPLS Morrison 6 12 2015 Page 72
42. y Graphical with LCD backlight 9 7 x 7 1 cm 3 8 x 2 8 in Printer optional 40 column Internal text and graphics Extemal USB printer HP PCL 3 0 and greater Connectivity USB Type A port for USE memory device front panel USS Type B port for optional computer connectivity rear panel USB A port for extemal printer rear panel Dimensions 30 W x 40D x 25 Hem 11 8 x 15 7 x 9 8 in Weight 8 6 kg 19 Ibs Power Requirements Selected Automatically 100 240 V 50 60 Hz STLCC CPLS Morrison 6 12 2015 Page 22 Spectrophotometer Genesys Keypad Key or button Function Accepts highlighted entered or selected values Advances to the next parameter or screen Prints the method or results to the selected printer If PC is selected for the printer sends the method or results to the USB port Displays a menu of software applications Displays the Utility screen Controls the location of the cursor Highlights the value or option for the selection O O 0 Enters numbers a decimal point and a minus sign for values I 8 9 9 9 Called Function keys Performs a specific function as displayed above cach key Functlon kcys Functions will change depending on the software screen Q Some function keys may not be active Cell position keys Clears the value being entered Selects the cell holder position to be measured e Returns to the previous screen B bl
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