Model EP-1 Econo Pump Instruction Manual - Bio-Rad
Contents
1. O 0 8 OL 3 32 pled ag 4 Vee ao 5 AD Aje V Section 7 Operation with other Instruments Press Calibration key until CAL is displayed Press the Run Stop key Pump will run for 5 minutes at 25 of maximum speed and the display will count down time When pump stops read the volume of fluid on the graduated cylinder Using the Arrow keys enter this amount on the display Alternatively when a suitable volume of liquid has been collected pressing Run Stop will turn off the timer and stop the pump Enter volume collected as described When the value on the display matches the volume collected in the graduated cylinder press Calibration The CAL indicator light will glow steadily indicating that the pump is calibrated DO NOT select a tubing size at this point even if you are using one of the standard sizes The pump will remember the calibration key even if it is switched off or disconnected from the power line Set desired flow rate using arrow keys Note Flow rate depends on condition of tubing platen adjustment and back pressure For maximum flow rate accuracy it may be necessary to recalibrate as the tubing ages when the tubing is replaced if the platen is read justed or if back pressure changes significantly The Model EP 1 Econo Pump can be used with other chromatography components Model 2110 and Model 2128 fraction collectors or with the BioLogic2. The base of the Model EP 1 Econo Pump contains a 40 pin connector for connection of the Econo Pump to the discontinued Econo System Controller This connector is not currently used with any systems sold by Bio Rad Laboratories and should remain unused Section 5 Tubing Selection and Installation 5 1 Tubing Selection The Model EP 1 Econo Pump may be used with most flexible tubing having an inner diameter less than or equal to 3 2 mm 1 8 and a wall thickness of 1 0 mm or less including silicone Tygon and PharMed Silicone tubing the most inert of the three is suitable for aqueous and polar solutions Tygon is suitable for most aqueous solutions It will generally have the shortest lifetime of the three PharMed is the longest lasting of the three and will provide the most consistent flow rate over time Table 5 1 shows approximate flow rate ranges with different tubing sizes Flow rates above 20 ml min may be obtained by plumbing two channels of 3 2 mm tubing through the pump and joining them at the output Table 5 1 Approximate Flow Rate Ranges ibing Size Flow Rate Range ml min D x Wall 0 1 2 3 4 5 6 78910 2 3 4 5 678910 20 0 8 mm 1 6 mm 3 2 mm Tubing length and the amount of tubing prestretch have a significant effect on both flow rate calibration and reproducibility of flow The PharMed tubing supplied with the Econo Pump has been cut to a predetermined length to accommodate tubing prestretch Silicon tubing in
3. Model EP 1 Econo Pump Instruction Manual Catalog Numbers 731 8140 731 8142 For Technical Service Call Your Local Bio Rad Office or in the U S Call 1 800 4BIORAD 1 800 424 6723 Warranty Model Serial Number Date of Delivery Warranty Period The Model EP 1 Econo Pump is warranted for 1 year against defects in materials and workmanship If any defects should occur during this warranty period Bio Rad will replace the defective parts without charge However the following defects are specifically excluded l 2 Defects caused by improper operation Repair or modification done by anyone other than Bio Rad Laboratories or their authorized agent Use with tubings or fittings not specified by Bio Rad Laboratories for use with this pump Deliberate or accidental misuse Damage caused by disaster Damage due to use of improper solvent or sample This warranty does not apply to tubing fittings and fuses For inquiry or request for repair service contact Bio Rad Laboratories after confirming the model and serial number of your instrument For Technical Service Call Your Local Bio Rad Office or in the U S Call 1 800 4BIORAD 1 800 424 6723 Section 1 Section 2 Section 3 3 1 3 2 Section 4 4 1 4 2 4 3 Section 5 5 1 5 2 5 3 Section 6 Section 7 7 1 7 2 7 3 Section 8 Section 9 Section 10 Appendix A Appendix B Appendix C Table of Contents Safety cics
4. 0 8 mm ID 1 0 mm wall 10 m 731 8208 PharMed Tubing 1 6 mm ID 1 0 mm wall 10 m 731 8209 PharMed Tubing 3 2 mm ID 1 0 mm wall 10 m 731 8240 Silicone Tubing Kit 0 8 mm ID 20 precut lengths and 4 sets of fittings 731 8241 Silicone Tubing Kit 1 6 mm ID 20 precut lengths and 4 sets of fittings 731 8242 Silicone Tubing Kit 3 2 mm ID 20 precut lengths and 4 sets of fittings 731 8247 PharMed Tubing Kit 0 8 mm ID 20 precut lengths and 4 sets of fittings 731 8248 PharMed Tubing Kit 1 6 mm ID 20 precut lengths and 4 sets of fittings 731 8249 PharMed Tubing Kit 3 2 mm ID 20 precut lengths and 4 sets of fittings 22 Catalog Number Product Description 731 8220 Low Pressure Fittings Kit includes over 250 male and female luer connectors 2 and 3 way stopcocks and tubing connectors Cables 731 8261 System Cable 1 8 pin mini DIN to DB 9 connector For connection of Model 2110 Fraction Collector to the Econo Pump to the BioLogic LP 731 8262 System Cable 2 8 pin mini DIN to 8 pin standard DIN For connection of Model 1327 Chart Recorder to the Econo UV Monitor 731 8264 System Cable 4 8 pin mini DIN to banana cable To con nect Econo UV Monitor to the Model 1327 Chart Recorder 731 8267 System Cable 7 8 pin mini DIN to bare wires To connect Econo Pump to the BioLogic System Model 2110 Fraction Collector 731 8122 Model 2110 Fraction Collector 100 120 V 731 8120 Model 2110 Fraction Collector 220 2
5. control a programmed gradient or fraction collection method When the indicator light is flashing pressing this key starts the method When the indicator light is lit pressing this key will interrupt the program which can be continued by pressing again When the LED displays FAIL indicating a brief power outage has occurred pressing this key continues the program Note When entering values for tubing calibration or fraction collection procedures only the flashing keys are active 4 2 Rear Panel Functions FUSES TYPE T 100 120v 0 804 d gt 220 240V 0 25 A Lag FREQUENCY 50 60 Hz Made in U S A Vv FOR CONTINUED FIRE PROTECTION USE MAX POWER S5VA ONLY SPECIFIED TIME DELAY FUSES gt aux v v Fig 4 2 Rear panel sockets Display Function Power Entry Module A Fraction Collector Output v Grounded 3 pin receptacle for the power cord Also contained within is a four position 100 V 120 V 220 V 240 V line voltage selector See Section 3 2 This 8 pin connector sends an advance signal to the Model 2110 Fraction Collector via System Cable 1 Model SV 3 diverter valve to divert y flow between collection devices Diverter Valve Output This 8 pin outlet sends a signal to the AUX Auxiliary Output This 8 pin connector is for communi y cation with the Model EM 1 Econo UV Monitor and the BioLogic System 4 3 Pump Base Features
6. precut lengths is also available from Bio Rad See ordering information in appendix C 8 When using tubing other than precut tubing from Bio Rad the tubing must be cut to the following lengths to accommodate prestretch Tubing Length Tygon PharMed 179 mm 1 5 7 04 0 05 Silicone 171 mm 1 5 6 75 0 05 Install tubing onto the pump head as described in Section 5 2 Warning If using tubing other than the type supplied by Bio Rad make sure the wall thickness is not greater than 1 0 mm Using tubing with a greater wall thickness can damage the pump and void your warranty 5 2 Tubing Installation 1 Referring to Figure 5 1 pull the platen cam lever away from the pump head to unlock the platen and slide the platen away from the pump head frame assembly exposing the rollers Fig 5 1 Tubing installation 2 Slip a lock ring onto one end of the tubing See below for the size and color of each lock ring Insert a barbed female luer fitting into the same end until the tubing reaches the flange of the fitting Clamp the luer fit ting into place by sliding the lock ring along the tubing over the barbed fitting see Figure 5 2 Repeat on the other end of tubing Note Use of lock rings is required only when operating at pressures above 10 psi Lock Ring Color Tubing ID Red 0 8 mm 1 32 Orange 1 6 mm 1 16 Yellow 3 2 mm 1 8 Z a Fig 5 2 Attachment of tubing fittings 3 Insert one end of
7. programming the V system and for selecting which parameter to display when running the pump ml O min O ml min O O Indicator Lights Run Stop Key Purge Key Direction Key Tubing Calibration Key Fraction Collector Key These lights indicate the units for the value displayed on the LED display located to the left A flashing light indicates that a value can be set using the Arrow keys This key is used to start or stop the pump It also has secondary functions such as holding or aborting a method This key is used to prime and purge tubing lines without disturbing the primary speed setting of the pump When Purge is pressed the pump will run at maximum speed 25 rpm The purge key will not operate during a fraction collection method This key changes the direction of pump head rotation The Econo Pump must be stopped before the direction can be changed This key is used to select pre pro grammed calibration settings for three tubing sizes or allows user calibration for increased flow rate accuracy or non standard tubing sizes The pump must be stopped for this key to operate This key is used to set fraction size void volume to move a diverter valve to collect or waste positions and total run volume The total run vol ume V may be used to program the pump to automatically shut off after a given volume has been delivered Program Run Key O a This key is used to
8. the tubing into the tubing retaining bracket of the pump head Lightly pull the tubing around the rollers to remove slack Attach the other end of tubing into the tubing retaining bracket on the opposite side of the pump head 4 Slide the platen back into the pump head frame assembly until it rests up against the tubing Press the cam lever in toward the pump head locking the platen up against the tubing and rollers Note that the platen can be inserted with the cam lever on the left or the right 5 3 Platen Adjustment Proper adjustment of the platen pressure increases flow stability minimizes flow pulsation and prolongs the life of the tubing Once the tubing is installed in the pumphead loosen the platen adjustment screw located on the front of the platen counterclockwise until the stop is reached For one piece of either PharMed or Tygon tubing the platen is properly adjusted at three to four full turns clockwise from stop For one piece of silicon tubing the platen is properly adjusted two full turns clockwise from stop When using two pieces of tubing approximately 50 more turns clockwise from stop will be required 10 Note Overtightening the platen adjustment screw will reduce flow rate and shorten tubing life If the platen is too loose flow rate will decrease as backpressure increases Section 6 Stand Alone Operation The Model EP 1 Econo Pump functions as a stand alone pump By specifying the pump tubing size it
9. 1 15 x pouca any FOR CONTINUED FIRE PROTECTION USE MAX POWER SSVA ONLY SPECIFIED TIME DELAY FUSES FUSES TYPE T 100 120V 0 50 A es 220 240V 0 25 A WN FREQUENCY 50 60 oo AUX v v v Mode in U S A Outlet to Waste Outlet to Collector Fig 7 1 Connection of the Model SV 3 Diverter Valve 7 2 Use of the Econo Pump with the Model 2128 Fraction Collector The Econo Pump is capable of controlling the Model 2128 Fraction Collector The pump must be calibrated in order to run a fraction collector program See Section 6 for calibration instructions Use System Cable 15 catalog number 731 8286 to connect the Econo Pump to the Model 2128 Collector If the SV 3 diverter valve is used connect it as shown in Figure 7 1 Ifthe Model 2128 Diverter Valve is used connect one end of System Cable 3 to the diverter valve port on the Econo Pump and connect the other end to the mini DIN port on the Model 2128 Fraction Collector This cable transmits flow diversion information from the Econo Pump to the collector Program collection as demonstrated in Section 7 1 For more information about the Model 2128 Fraction Collector consult the user manual 16 7 3 Use of the Econo Pump with the BioLogic System The Econo Pump can be controlled by the BioLogic System and used as a sample loading pump Note The Econo Pump must have v2 12 software in order to accept on off commands from the B
10. 40 V Model 2128 Fraction Collector 731 8123 Model 2128 Fraction Collector 100 120 V 731 8124 Model 2128 Fraction Collector 220 240 V Bio Rad sells a complete line of integrated chromatography systems for protein purification For more information on the BioLogic System family of products contact your local Bio Rad representative PharMed and Tygon are the registered trademarks of the Norton Company 23 z071 Bio Rad Laboratories Life Science Web site www bio rad com USA 800 4BIORAD Australia 02 9914 2800 Group Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 21 507 6191 Canada 905 712 2771 China 86 10 8201 1366 68 Denmark 45 44 52 1000 Finland 358 0 9 804 2200 France 01 47 95 69 65 Germany 089 318 84 177 Hong Kong 852 2789 3300 India 91 124 6398112 113 114 Israel 03 951 4124 Italy 34 91 590 5200 Japan 03 5811 6270 Korea 82 2 3473 4460 Latin America 305 894 5950 Mexico 52 5 534 2552 to 54 The Netherlands 0318 540666 New Zealand 64 9 4152280 Norway 47 23 38 41 30 Russia 7 095 979 98 00 Singapore 65 2729877 Spain 34 91 590 5200 Sweden 46 8 55 51 27 00 Switzerland 061 717 9555 United Kingdom 0800 181134 Sig 1200 M7318140 Rev J
11. System the high resolution liquid chromatography system Each of these applications is discussed below 12 7 1 Use of the Econo Pump with the Model 2110 Fraction Collector The Econo Pump is capable of controlling the Model 2110 Fraction Collector The pump must be calibrated in order to run a fraction collector program See Section 6 for calibration instructions Use System Cable 1 catalog number 731 8261 to connect the Econo Pump to the fraction collector Connect the 9 pin D connector to rear of the Model 2110 and the mini DIN plug to the rear port of the Econo Pump with the collector icon See Figure 4 2 To program a simple time based collection method follow these instructions 1 Press the Fraction Collector key on the pump front panel to enter the fraction collector Edit mode Note that the fraction size and ml indicator lights are flashing prompt ing entry of fraction size Enter the fraction size in 0 1 ml increments using the Arrow keys After fraction size is selected press the Fraction Collector key once more to confirm the fraction size 2 Enter a void volume V in 1 ml increments using the Arrow keys This feature allows the user to begin col lecting fractions only after a pre set volume of liquid i e the void volume has passed through the column Press the Fraction Collector key to confirm this value If a void volume is not desi
12. allows you to enter a flow rate rather than a nominal pump speed setting An uncalibrated Econo Pump will display pump speed as percent of maximum rpm There are two ways to calibrate the pump through a preset tubing size or through a user calibration procedure Flow Rate Calibration with Pre Selected Tubing Sizes 0 8 1 6 and 3 2 mm ID 1 With the pump in Stop mode install tubing as described in Section 5 2 ou OI 2 Press the key in the lower left front of the pump panel o aa o corresponding to the pump calibration feature and continue pressing until the indicator light corresponding to either 0 8 1 6 or 3 2 mm ID tubing is lit mo 3 The LED display reads ml min for any of these pre pro min O Hin grammed tubing settings If improved flow rate accuracy is desired see User Calibration Procedure below C3 D co oe 4 The pump is now automatically calibrated for the specific bed tubing size Press the Run Stop key to start flow Increase mi O ain or decrease the pump speed with the Arrow keys mimin J User Calibration Procedure 1 Install tubing as described in Section 5 2 Connect pump inlet to a container of water or buffer Connect a length of tubing to the pump outlet rO 2 Run pump to purge air from tubing then stop pump o Place the end of the outlet tubing in an empty graduated cylinder 11
13. ation and Down Arrow keys while turning the pump back on Hold the two keys until the display stabilizes 19 Appendix A Technical Specifications Number of channels Flow rate range per channel Pump head speed Tubing diameter Speed adjustment Speed stability Maximum counterpressure Motor Line voltage Dimensions Weight Operating temperature Material of construction Regulatory Compliance 20 2 0 1 20 ml min depending on tub ing diameter 25 rom maximum 0 4 mm ID to 3 2 mm ID 1 1 of full scale 30 psi 2 kg cm2 or bars DC speed controlled 55 watts 90 132 VAC 180 265 VAC 47 63 Hz 143 x 202 x 222 mm W x D x H with pumphead 3 3 kg 4 to 40 C polypropylene and other solvent resistant plastics CSA C22 2 No 151 M1986 TUV EN61010 1 1993 CE EN55011 1991 Class A EN50082 2 1995 Appendix B Operation of the Model EP 1 Econo Pump When Connected to Other Instruments The Model EP 1 Econo Pump provides a variety of output signals which can be used to control the operation of non Bio Rad fraction collectors or to communicate with non Bio Rad UV monitors and recording devices Communication is accomplished through two standard 8 pin mini DIN sockets on the rear panel of the pump The third socket is for the operation of the Model SV 3 Diverter Valve see Figure 4 2 Bio Rad offers an accessory breakout cable System Cable 7 which has an 8 pin mini DIN connector at one end a
14. cesicsesscecsoscontscodnecssasesedsssvessosedecedsedossbesessesesssceseses 1 Introduction scccsccssscssscsssssccescssseccssscssscessnsssssseenees 2 Unpacking and Setting Up ssccsssssscsseeescceeseees 3 Unpacking Instructions ccccccesceeseceecseceseeeceseeeecesceeeneeees 3 Voltage COonversion csccescceccesecesceseseeceseeeeceseeeeeeseeseeeseeneeenes 4 Physical Description and Control Features 5 Front Panel Functions konsene nie ani ni 5 Rear Panel Functions c cceccescescesseeeceseeeeceseeeeceseeneesaeeneeenes 7 Pump Base Features iis cccccsccsesssccceccessceveccveceevsasevecieinouiweceensvsese 8 Tubing Selection and Installation ccccscsssosseees 8 Tubing Selections nnn iniri ini 8 Installation of Precut Bio Rad Tubing ssseeseeeeeseeeeseseeses se 9 Installation of Uncut Tubing eceeseeceeeereeseeeneeneeenees 10 Stand Alone Operation cccsccsccsssccecssscesceeseecees 11 Operation with other Instruments c cscssceceseee 12 Use of the Pump with the Model 2110 Fraction Collector sse menneen e Abe tn aes 13 Use of the Econo Pump with the Model 2128 Fraction Colle ctr ecreis ieie enis e asaan 16 Use of the Econo Pump with the Biologic System ireen naa ree E aa ae aA 17 Cleaning and Maintenance eesssessosssesssesssessessseessees 17 Troubleshooting eessessesesoeesoesooesoossoosssesssesssessesssessseoo 18 Embedded FunctionS
15. ction collector parameters such as fraction size void volume and total run volume A membrane key panel with graphic icon displays allows easy user interface Section 3 Unpacking and Setting Up 3 1 Unpacking Instructions Carefully remove the unit from the shipping box lifting from the handle on the back of the pump or from the bottom of the instrument Remove the plastic bag and inspect the instrument for any external damage Parts included with the Model EP 1 Econo Pump are illustrated in Figure 3 1 Check off all parts against the supplied packing list Your Model EP 1 Econo Pump was carefully tested at the factory and was shipped in good working order If any part is missing or damaged contact Bio Rad Laboratories immediately Refer to Figure 3 1 for the proper identification designation of any missing or damaged part s Tubing and Fitting Starter Kit Power Cord Model EP 1 Econo Pump Also includes Instruction Manual Fig 3 1 Parts supplied with the Model EP 1 Econo Pump 3 2 Voltage Conversion Ay Warning The Model EP 1 Econo Pump is shipped in its 120 V or 220 V version To operate at other voltages refer to the procedure below Failure to follow this procedure may result in damage to the unit and invalidation of the warranty Prior to connecting the power cord to the power entry module and wall outlet verify that the voltage indicated on the power entry module matches your line voltage If it does no
16. es for obstructions Check pump tubing for damage Replace damaged tubing Replace tubing Adjust platen Re select tubing size on front panel of pump Problem Possible Cause Solution Large change in Re calibrate flow back pressure or fluid viscosity Restriction on inlet Remedy side Excessive tubing Platen too tight Turn platen adjustment screw wear clockwise to stop flow then clockwise until flow starts Cannot calibrate Pump must be Insure that pump is stopped pump stopped in order to calibrate pump Error message Contact Bio Rad Technical displayed upon Service powering up ER Section 10 Embedded Functions During a technical service call you may be asked to perform tasks which require you to press combinations of keys These tasks are defined below 10 1 Software Confirmation To check the version of software installed in the Econo Pump simul taneously hold down the direction key in the upper left corner and the down arrow key on the front panel of the Econo Pump The four digit display should display v2 01 or higher 10 2 Warm Reboot You may be asked to perform a warm reboot if the Econo Pump soft ware is not functioning properly To initiate a warm reboot press the Tubing Calibration and Down Arrow keys simultaneously and hold for 3 seconds 10 3 Cold Reboot A cold reboot may be necessary if the warm reboot was unsuccessful Turn off the pump and hold the Tubing Calibr
17. g Remove the platen and relax the tubing by unhooking one end of the tubing from the tubing bracket To prevent the formation of precipitates around the pump head and on the membrane key panel promptly remove any spills Clean with deionized water 17 Section 9 Troubleshooting Problem Possible Cause Solution Pump displays OUT Pump displays FAIL Pump will not run no LEDs lit Liquid remains stationary in tubing Flow rate not consistent Power surge or brownout Power outage No power Fuses Platen not adjusted Clogged tubing valves or fittings Damaged tubing Pump tubing damaged or worn out Platen adjustment incorrect Wrong tubing size selected 18 1 Turn the power switch off 2 Press and hold down the down arrow key and the CAL key simultaneously while turning the power switch on This will reset the pump If the pump was previously calibrated you will have to recalibrate it If you wish to continue a run in progress press program run key Otherwise press the run stop key Verify that powercord is plugged in and the power switch is turned on Check that proper fuses are installed and intact Insure that the calibration indica torlight is not flashing For pump to run this light must either be off or steadily lit Platen too loose Turn platen screw clockwise just until liquid begins moving within tubing Check tubing fittings and valv
18. he Arrow keys to display the ml of output the progression of the collection in min the flow rate in ml min or the percent of total run time To abort the fraction collection scheme at any time during the program press the Run Stop key twice The pump will stop and the LED display will read OFF indicating abortion of the fraction collection program Note When a program is aborted it can only be restarted from the beginning To place a collection program that has begun on Hold press the lit Program Run key once The pump will stop and the program will be held To resume the collection program press once again Pressing the Run Stop key while the program is on hold will abort the fraction collection scheme When the fraction collection program is complete the pump stops and the display reads END Press any key to return to the ready mode Notes on Fraction Collection 1 After a fraction collection program has begun the pump cannot be turned off without terminating the fraction collection program 14 2 During the V period of the fraction collection scheme as indicated by the V indicator light the fraction collector is not active To collect fractions during this void volume period cancel V by pressing the Fraction Collector key The fraction collector will start with the LED display indicating the time progression of the collection scheme 3 When the fraction collector is disabled by pressing the Fraction Col
19. ioLogic System See Section 10 1 for instructions on how to check the software version If an upgrade is required contact your local Bio Rad representative To connect the Econo Pump to the BioLogic use System Cable 7 catalog number 731 8267 Connect the mini DIN end of the cable to the AUX port of the Econo Pump and make a bare wire connection to the AUX port of the BioLogic Workstation RED to port 6 BLUE to port 9 To connect the wires to the AUX port of the BioLogic System turn the small flathead screwdriver counterclockwise to back out the screw Insert the bare wire into the port and turn the screwdriver clockwise to secure the connection Be sure to calibrate the pump and set a flow rate prior to using the Econo Pump with the BioLogic System since the BioLogic sends only on off commands to the Econo Pump To program the Econo Pump as a sample loading pump with the BioLogic System refer to the BioLogic System user manual Section 8 Cleaning and Maintenance The Model EP 1 Econo Pump requires very little maintenance to assure reliable operation The procedures outlined below will insure maximum pump life e Check tubing regularly for signs of cracking and wear If any exist replace the tubing e For optimal tubing life use a slow to medium pump speed To increase throughput increase tubing size to obtain the desired output e When not in use tubing should be removed from the pump to prevent deformation of the tubin
20. lector key the fraction collector carousel will advance one tube 4 When the fraction collector indicator light on the pump is lit the Model 2110 Fraction Collector will be put into remote mode Only the manual advance keys will be active and the Model 2110 display reads 5 The fraction collection control feature of the Model EP 1 Econo Pump can be used to turn off the pump even if a fraction collector is not connected Simply enter 0 for fraction size After confirming the V value entered the V light will be lit solid start the pump if not already running then press the flashing Program Run key The LED display will show time elapsed The pump will stop automatically when the total run volume has been delivered Use of the Diverter Valve The optional Model SV 3 diverter valve catalog number 731 8235 provides a means to divert the eluant stream from a fraction collector to a waste receptacle or other collection device during a fraction collection program If the void volume function V of the collection scheme is enabled with the diverter valve in place the eluant stream will be diverted away from the fraction collector during that period When that period is over the eluant stream will be diverted to the fraction collector Whenever the diverter valve changes from waste to collect or vice versa an event mark is generated This will appear as a deflection on the chart recorder For connection of the diverter valve see Figure 7
21. nd loose wires on the other for connection to other instruments Output signals are TTL compatible To use these signals you must insure that the circuit external to the Model EP 1 Econo Pump does not draw more than 15 milliamperes of current The following table describes the two rear panel mini DIN connector pinouts Table B Rear Panel Pinouts i gt AUX y v Pinout Signal Type Pinout Signal Type i Shield Shield 1 Fraction Advance 1 Start Enable low going output output 2 No Contact 2 No Contact 3 No Contact 3 Mark A output 4 No Contact 4 No Contact 5 Fraction Advance 5 No Contact high going output 6 No Contact 6 Pen output 7 Ground 7 Ground 8 Fraction Advanced Marks to 8 Mark B output Recorder low going input 21 Appendix C Ordering Information Catalog Number Product Description Model EP 1 Econo Pump 731 8140 Model EP 1 Econo Pump 110 V USA power cord with tubing set and starter fittings kit 731 8142 Model EP 1 Econo Pump 220 V no power cord with tubing set and starter fittings kit 731 8145 Model EP 1 Pumphead Assembly 731 8235 Model SV 3 Diverter Valve Tubing and Accessories 731 8210 Silicone Tubing 0 8 mm ID 0 8 mm wall 10 m 731 8211 Silicone Tubing 1 6 mm ID 0 8 mm wall 10 m 731 8212 Silicone Tubing 3 2 mm ID 0 8 mm wall 10 m 731 8214 Tygon Tubing 0 8 mm ID 0 8 mm wall 10 m 731 8215 Tygon Tubing 1 6 mm ID 0 8 mm wall 10 m 731 8207 PharMed Tubing
22. red enter 0 Note The optional Model SV 3 Diverter Valve catalog number 731 8235 is required to use the void volume feature of the Model EP 1 Econo Pump oh 3 Select the total run volume V in 1 ml increments rO using the Arrow keys If zero is entered and confirmed mn aA ae the fraction collection scheme is disabled To resume V Deeg data entry see Step 1 above All values previously entered remain in the memory until other values are entered to replace them Note The Econo Pump will automatically shut off upon reaching the total run volume V 13 Gop E mn Jor J Q odo C3 ae m JIC End After selecting a V value press the flashing Fraction Collection key to enter the value The flashing LED display shows the estimated number of fractions to be collected Press the flashing Fraction Collection key once more to enable the fraction collector To actuate the fraction collector press the Program Run key after starting the pump The fraction collector can be programmed with the pump running or stopped After the fraction collection program is initiated the LED display shows the progression of the fraction collection scheme in minutes to the nearest tenth of a minute While a program is running the user can use t
23. seseessesseroessessoseesoesoessesoesossseeoee 19 Technical Specifications essoessoessosssessoesssessesssessseeo 20 Operation of the Econo Pump When Connected to Other Instruments e sesosssesssrosssesoesseeo 21 Ordering Information eeesosssoessoessosssessossssessessseossese 22 Section 1 Safety A Disconnect supply before servicing No user serviceable parts inside refer servicing to Bio Rad service personnel This instrument is intended for laboratory use only This product conforms to the Class A standards for electromagnetic emissions intended for laboratory equipment applications It is possible that emissions from this product may interfere with some sensitive appliances when placed nearby or in the same circuit as those appliances The user should be aware of this potential and take appropriate measures to avoid interference Section 2 Introduction Fig 2 1 Model EP 1 Econo Pump The Model EP 1 Econo Pump is a two channel bi directional variable speed peristaltic pump for low pressure chromatography and general laboratory use It offers a full range of features to facilitate ease of use as a stand alone pump or as a part of the BioLogic System As a stand alone pump the Model EP 1 Econo Pump delivers flow rates from 0 1 to 40 ml min with the ability to self calibrate the flow rate for 0 8 1 6 and 3 2 mm ID tubing displaying pump output in ml min The pump can be programmed to control fra
24. t use the following procedure to make the conversion Refer to Figure 3 2 l 2 3 Disconnect the power cord from the unit Remove the fuse drawer with a small blade screwdriver or similar tool Pull the fuse holder out of the fuse drawer and if necessary replace the fuses with ones having the correct current rating Use 0 50 A fuses for 100 V and 120 V operation 0 25 A fuses for 220 V and 240 V operation Rotate the voltage selector through the window in the fuse drawer until the proper voltage shows Reinsert the fuse drawer into the power entry module with the locking tab to the left The voltage indicator will read right side up if the drawer is oriented correctly Press gently until it snaps into place Insert the power cord plug into the power entry module Plug the power cord into a properly grounded outlet Entry Module Voltage Selector Fuse Draw r Window Fig 3 2 Voltage conversion Section 4 Physical Description and Control Features 4 1 Front Panel Functions lok ECONO PUMP 3 3 min O ml min O O Fig 4 1 Front panel controls Display Function LED Display The four character display will show 8888 various parameters during programming and operation of the Econo Pump The indicator lights located immediately to the right of the display indicate the units displayed ml minutes ml minute or percent A Arrow Keys These keys are used for setting system parameters when
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