GOW-MAC Series 600 GC
Contents
1. GOW MAC Series 600 Gas Chromatograph GC May 14 2008 Instrument instructions can be found at http academic bowdoin edu chemistry resources instructions shtml If you have any problems with the instrument or would like to get trained please contact Celeste Moody 725 3756 cmoody bowdoin edu Druckenmiller 256 1 Protocol a Read instructions carefully before using instrument Reading the bold sentences in each category will tell you what you need to know to run the instrument i Bullets are under the bold sentences when more detail is required ii At the end of the instructions is a frequently asked questions troubleshooting section 2 Method Development a Methods are developed using the Edit soft key on the Home page b Depress the Edit key You will be presented with a choice of what part of the method to edit Timed events are set outside the analytical period these must be set up separately c Use the gt key to move the arrow to chromatography method press Do This d The screen will now show the equipment available for your method 3 Isothermal Run a Move the gt to Detector 1 press Do This b Move the gt key to Detector Temperature use the Up and Down Keys to change the temperature Press Accept when the desired temperature is reached c Move the gt to Injector 1 on the Edit Chromatography screen and press Do This d Use the Up and Down keys to set the desired temperature Press Acc2. d the heat turned on the GC starts toward the parameters called for in that method During this period the Not Ready signals is displayed at the top left of the screen As soon as the GC is ready for an injection the Ready message will appear in the top left of the screen You are now ready to make an injection and initialize a run by pressing the Run key on your Action page 2 8 Running a Method When Not Ready a When you want to start a run even though the GC is not ready you can from the Action page There are three soft keys on the Action page i Run identical in operation to the Run key ii R C Run Confirm is similar to the run key except that pressing the Run soft key first and then the R C will start the GC whether the system is ready or not iii Shutdown this button is used to cool the oven down in a controlled way That is the fans remain running the vent door is open and the heaters and detectors are turned off b Therefore to start a run when the GC is not ready first go to the Action page then press the Run key followed by the R C key Integrator Functions LIST LIST lists current run parameters REA ENTER checks system readiness SY ENTER lists current system configuration START starts run PLOT starts plot without integration STOP stops run or plot SHFT SEQ START Starts a sequence of runs CHT SP 1 ENTER sets chart speed to 1 cm min SHFT ENTER ad
3. e the Up and Down keys to set the first required rate Use the gt key to point to upper temperature Set this with the Up and Down keys The next press of the gt key advances to segment 2 If you wish only one ramp make sure the ramp rate for segment 2 is zero When the program is set press Accept until the Edit screen appears 5 Saving a Method a b When the method is complete press the soft key Save on the Edit page You will be presented with a menu which will allow you to save your new method First use the Up and Down keys to select the method number under which the method is to be stored When this number is chosen press Accept d Next move to the name field Use the Up Down and Accept keys to name the method Set the characters using the Up and Down keys and when correct press the Accept key to move to the next space Now press the Save soft key You will be asked to overwrite an existing method Proceed by pressing Save 6 Retrieving a Method a b C To retrieve a stored method press the soft key Retrieve on the Edit page A list of stored methods will appear Use the soft keys to locate the method you would like to retrieve When it is selected press Retrieve The selected method is loaded and becomes active 7 Running a Method a b Turn the heat on by pressing Home Then press the Action soft key followed by the Heat On key Once a method has been created or retrieved an
4. ept when done Select the Column Oven setting with the gt key and press Do This f When the screen changes select Isothermal and press Do This Use the Up and Down keys to raise and lower the oven temperature When the desired temperature is reached press Accept Press Home on the Main screen To turn on the heaters press Action on the Home page When the action screen comes up select Heat On i Monitor the temperature from the status screen found on the Home page 4 Temperature Programmed Run a Selecting Temperature Programmed presents you with a series of two menus b The first menu will ask you for an initial temperature and time The initial time is the time the oven will stay at the initial temperature after the run has started p09 With the gt alongside Initial Temperature use the Up and Down keys to set the required temperature Now move the gt alongside the Initial Time and use the Up and Down arrows to set the desired time d When they are at the desired values press Accept i The next screen will ask for the program parameters The GC is capable of doing a 10 segment ramp The segments are divided on two screens The second screen can be reached by pressing Accept The temperature program will terminate after the last segment with a nonzero value It will also terminate if the ramp rate for the next segment is set to zero regardless of what follows Move the gt to Ramp Rate in segment 1 us
5. vances the paper continuously LIST TIME ENTER lists all current timetable entries TIME 5 CHT SP 7 ENTER sets chart speed to 7 cm sec at 0 5 minutes into the run DEL TIME ENTER deletes entire timetable DEL TIME 1 ENTER deletes all entries for 1 min SHFT PREP SHFT METH prepares a method step by step SHFT EDIT SHFT METH edits an existing method SHFT press the Shift key BOLD words indicate a key that is pressed Everything else is typed in using the keypad Adapted from Series 600 Gas Chromatograph User s Manual by GOW MAC Instrument Co 1995 Adapted from HP 3395 Integrator Operating Manual by Hewlett Packard 1997
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