V200RS-UV200RS Series-OPR
Contents
1. Readings differ greatly from expected results Display not zero but does display some value Readings drift 1 Internal connection between the mode switch and the main PC board is bad 2 Mode switch is defective 3 Main PC board is defective 1 Internal connection between the sensitivity switch and the main PC board is bad 2 Sensitivity switch is defective 3 Main PC board is defective 1 Internal connection between the digital display meter and the main PC board 2 Main PC board is defective 3 Digital display meter is defective 1 Check wavelength calibration 2 Check your application procedure and sampling technique 1 Bad internal connection between the resistor of the 0 T control knob and main PC board 2 Detector is dirty or defective 3 Sample compartment is not securely seated in the instrument 4 Main PC board is defective 5 Digital display is defective 1 Check your application procedure and sampling 19 technigue 2 Fumes from sample are affecting the optics of the instrument 3 Power in the line is fluctuating or instrument 4 Instrument is not grounded properly 5 Line voltage is incorrect 6 Bulb is defective 7 Detector is defective 8 Main PC board is defective Readings are not repeatable 1 Check that the wavelength and 100 T are set correctly 2 Is there any bubbles and degassing in your sample 3 make sure that test tube is clean 4 Tub2. Carefully unpack the contents and check the materials against following packing list to ensure that you have received everything in good condition Packing List for 200 Series Spectrophotometer Table 2 Description Ouantity Spectrophotometer 1 set Square Cuvettes 1 box pk of 4 Dust cover 1pc User s manual 1 book Place the instruments in suitable location In order to have the best performance from your instrument Keep it as far as possible from any strong magnetic or electric fields or any electrical device that may generate high frequency fields Set the unit up in an area that is free of dust corrosive gases and strong vibration WN Remove any obstructions or materials that could hinder the flow of air under and around the instrument vibration PA Plug the power cord into an outlet Turn on the instrument and allow it to warm up for 15 minutes before taking any readings WN You spectrophotometer is now ready for operation SELECT LIGHT SOURCE for UV only This function is only used for UV 200 RS spectrophotometer You may use the FUNC key to selecting an available lamp for great measuring depend you samples The light sources can be exchange between 325nm to 355nm E Press the FUNC key until display shows LAMP EXCHANGE 340nm P Press ror key for setting the wavelength point of light source exchanging Press ENT key to store the parameter to be
3. entered TURN ON OFF THE LAMP The lamps of UV 200 RS spectrophotometer may turn on or off the lamp if needed P Press the FUNC until display shows W _lamp on W lamp off or D2 lamp on D2 lamp off then press the ENT key to turn on or off the lamps CONNECT INSTRUMENTS TO PC The 200 series spectrophotometers has been built in RS 232C interface it enables you to connect a personal computer and use PC running Windows 98 to operate your spectrophotometer When the instrument is connecting to the PC the display is showing CONNECTING PC When the instrument is connected to the PC the display is showing PC CONNECTED When the instrument is not connected to PC the display is showing CONNECT PC Press the FUNC key until display shows CONNECT PC then press the ENT key OPERATING FEATURES 1 DIGITAL READOUT displays the data of Transmittance Absorbance Concentration 2 3 WAVELENGTH SELECTOT KEYS used to select the desired analytical wavelength 9 and used to select the special functions as setting the parameter of standard concentration concentration value or factor light source exchange selection Lamp ON OFF etc 4 FUNCTION SELECTOR select the functions as setting the parameter of standard conc
4. filter in the well of the cuvette holder record the T reading on the digital display Check the wavelength calibration with Didymium Filter Repeat steps EJ through A to measure separately the wavelength of the point between 519nm and 539nm 16 FEW Looking for the minimum Transmittance along this record the minimum Transmittance should be obtained between 526nm and 532nm The wavelength accuracy of the SP 2100 series spectrophotometers is 2nm TROUBLESHOOTING Problem Display shows 1XX X in Yotransmittance mode and shows a negative reading in absorbance mode Display shows 000 0 in T mode or 1 XXX in absorbance mode where XX are two blank digitals What to do 1 Check the sample compartment lid is closed or not make sure that the lid is closed 2 Make sure that the sample compartment is empty and the light door is shut 3 Adjust the sensitivity switch to low position 4 Turn 100 T A knob until display is on scale 1 Check the bulb if it is not lit replace the bulb 2 Check whether or not tube is inserted the sample compartment if not insert it Note there is a light door is built in the front of the sample compartment To set full scale you have the insert the tube with blank solution 2 Check whether or not light affects the reading O Open the lid of sample compartment make sure nothing block in the light path OCheck the light door with a tube if
5. measured ANote Solution must be at least 20 mm high in a standard square cuvette 11 7 0 Close the sample compartment cover put the blank solution into the light path set 100 T or OAbs by pressing the 0Abs 100 T KEY until display reads 100 0 T or 0 000A 8 0 You may then index the cuvette holder by pulling the cuvette selector arm into the next position and reading the T or Abs value of the samples CONCERTRATION MODE QNote This mode is used only if the linearity of the standard curve has been verified Using in C Standard mode 9 0 Press MODE selector key until the Abs is displayed 10 0 Place the blank solution in the sample compartment then close the sample compartment cover 11 0 Press the 100 T OA control key to zero the blank solution 12 0 Remove the blank solution from the sample compartment cover 13 0 Press the FUNC selector key until the the STD CONC 19099 jis lit 14 0 Using the r or O key to setting the concentration of the standard on the digital display then press the ENT key 12 15 0 Insert the sample solution to be measured into the cuvette holder Close the sample compartment cover read results directly in concentration units on the digital display Using in C Factor mode 9 0 Press MODE selector key until the LED underthe Abs is lit 10 0 Place the blank
6. solution in the sample compartment then close the sample compartment cover 11 0 Press the 100 T 0A control key to zero the blank solution 12 0 Remove the blank solution from the sample compartment 13 0 Press the MODE selector key until the STD FACTOR 1999 is lit 14 0 Using the n or O key to setting the factor of the standard on the digital display then press the ENT key 15 0 Insert the sample solution to be measured into the cuvette holder Close the sample compartment cover read results directly in concentration units on the digital display 13 APINN TTEN GANO SE This spectrophotometer has been designed to be durable and reliable and reliable Therefore the routine maintenance of the instrument is minimal However you will need to replace the lamp or you may want check the wavelength calibration and photometric linearity to verify the instruments s pweformance NA ZS Warning The manufacturer recommends only authorized service representatives perform procedures reguiring removal of the instrument s cover and replacement of electrical component s To protecting both of yourself and your instrument be sure to contact MRC authorized service representative to perform any service procedure you do not feel comfortable performing KE Turn off the power and unplug the instrument EA Remove the two
7. emistry laboratory petrochemistry laboratory environmental protection and various fields of guality control This user s manual provides information of 200 series of spectrophotometers a list of main specifications and available accessories After carefully unpacking the content check the materials against the packing list to ensure that you have received everything in good condition If you find part is missing damaged or any other defect contact MRC sales representative immediately However to keep pace with technological advance the specifications and operating instructions may be modified or changed as needed MRC reserves the right to make design modification and changes SPECIFICATIONS Power Requirement Size mm Weight Standard Table 1 Model V 200 RS UV 200 RS Optical System Littrow type single beam grating system 1200line mm Spectral Siltwidth 4nm 5nm Wavelength Range 320 1000 nm 200 1000 nm Wavelength Accuracy 2 nm Repeatability 1nm Stray Radiant Energy lt 0 1 T 340nm lt 0 5 T 220nm and340nm Photometric Range 0 T to 125 0 T OA to 2 5A OC to 1999C 0 1999F Stability 0 002A hr Photometric Accuracy 0 008A 0 5A 0 004A 0 5A 115 230volt 10 60 50Hz adjustable 465 18 3 Wx365 14 3 Dx175 6 9 H 8 5kg 11 5kg Instruction manual Dust cover 10x10x45mm rectangular cell T A C application software for WIN 9X 5 UNPACKING INSTRUCTIONS P
8. entration concentration value or factor light source exchange selection Lamp ON OFF etc 5 ENTER KEY is used to entering the parameter of sample to the memory or confirming the function that is selected 6 100 T 0A CONTROL is used to set the display to 100 T or OAbs It must be reset whenever the analytical wavelength has been changed 7 MODE SELECTOR selects the Transmittance Absorbance Concentration mode 8 0 T CONTROL is used to set the display to 0 T before setting OAbs 9 CLEAN KEY is used for cleaning the wrong parameter 10 OPERATING PROCEDURES 1 2 Plug the instrument into a grounded outlet 2 0 Turn the instrument on Allow the instrument to warm up for at least 20 minutes 3 0 Set to desired wavelength with the WAVELENGTH UP and DOWN ARROWS 4 0 Select the desired operating mode as TRANSMITTANCE ABSORBANCE or CONCENTRATION when using the MODE key 5 0 Choose matched cuvettes of appropriate pathlength for analytical method you are using you have to use the same pathlength cuvette for all blanks standards and samples amp NOTE All of cuvettes supplied with your instrument are 10mm pathlength and have been matched Glass cuvettes are useable above 325nm only Quartz cuvettes must be used below 325nm 6 0 Fill one of the matched cuvettes with a blank solution and place in position one of the cuvette holder The other positions with sample solutions to be
9. es are not aligned in exactly the same position for each reading 5 Sample compartment is not securely seated in the instrument 6 Cuvette holder is not securely seated in the adapter 7 Diameter of tube may be too small for a snug fit the sample compartment MRC OFFICES P O B 1684 TEL AVIV 61016 ISRAEL TEL 972 3 5593105 972 3 5595252 FAX 972 3 5594529 20
10. mrc UV200RS V200RS SERIES Spectrophotometers UV 200 RS V 200 RS USER S MANUAL TABLE OF CONTENTS Introduction Specifications Unpacking Instructions Select the Light Source Turn On Off the Lamps Connect the Instrument to PC Operating Features Operating Procedures Maintenance A l N O 0N000 rN INTRODUCTION Thank you for your purchase of analytical instrument The 200 series spectrophotometers are single beam system The instruments are compact lightweight easy to use value and ruggedness A sleek new design houses up to date electronics and the latest optical components for improved performance capabilities 2 line 20 character liguid crystal display readout displays data and gives easily understood Large sample compartment accepts from 5mm to 100mm pathlength cuvette and various accessories Th built in RS 232 serial interface enables the instrument to be connected to a computer or printer Windows amp 98 based application software is available for 200 series spectrophotometer The new 200 series Spectrophotometers are available in two models an instrument which cover the Ultraviolet and Visible wavelength range from 200 1000nm and an instrument which cover the Visible wavelength range from 320 1000nm The nominal 5nm spectral bandpass are constant over the entire wavelength range The 200 series Spectrophotometers are ideal for use in a clinical laboratory bioch
11. screws on the back side of the instruments EJ Remover the lamp from lamp holder 14 EU Carefully replace the new lamp A Note Do not handle Halogen lamp with bare fingers Use a piece of tissue or cloth when handling the lamp The oil from your fingers can cause the lamp to burn out prematurely VAZA VEN SINE TAL PP NIE OD MN TOKOIN J gt SN N A VEIL SIN Grd CA LIISIRRA KONI DD As usual the 200 series spectrophotometer retains its wavelength calibration indefinitely However if the instrument receives a severe shock or is abused use the following methods to check the wavelength calibration In the Didymium filter method the filter has two special absorbance peak at the 529 nm and the 808nm when the instrument is calibrated properly you will find minimum Transmittance maximum absorbance at the range of 529nm or 808nm 2nm Note that the specific transmittance values are not important you are only looking for the wavelength where minimum transmittance maximum absorbance occurs EE Turn on your instrument and allow it to warm up for 20 minutes 2 Select the Transmittance operating mode by press the MODE key 15 K Set the wavelength to 519nm ZW Insert the cuvette filled with distilled water in the well of the cuvette holder R Pressing the 0Abs 100 T Key until display reads 100 0 T or 0 000A then remove the cuvette with water SX Insert the didymium
12. you cannot insert tube into sample compartment perhaps the door is blocked clean and remove anything in it 17 Nothing at all appears on the display Check the display to see if it changes when you open and close the lid If the display doesn t change Either the detector or the main PC board needs to be replaced Olf the display does change check the light beam with the following steps Set the wavelength to 580nm Place a test tube with a long strip of white paper into sample compartment you should see a yellowish light from the bottom of the paper coming from the right sight of the sample compartment If you dont see any light at all the internal optical path is blacked in some way if you see light check that cable from the detector to the main PC board is connected Replace detector assembly Replace main PC board 1 Check the power indicative lamp if it is not lit Power cable not plugged Power switch on unit is off or defective Power cable is defective Fuse is blown is located inside in the bottom of the instrument 2 Main PC board is defective 3 Internal power transformer is defective 4 Internal wiring connections are bad 18 No difference in the readings on the display when changing between Abs and Trans mode makes No difference in the readings on the display when changing sensitivity switch between high and low positions Bulb is lit but no display at all
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