Picofluor™ - Masterflex
Contents
1. Specification Description Size 1752x239 7 4 45 cm x 8 9 cm x 18 4 cm Weight 14 oz 0 4 kg Dynamic range 4 orders of magnitude Resolution 12 bits LCD Display 2 x 16 characters Case Meets IP 67 Standard dustproof and waterproof Temperature 41 104 F 5 40 C Detectors Photodiodes measurement capability from 300 1000 nm Calibration Type Single point and blank Alarms Low battery circuit failure high blank Cuvette Type 10 x 10 mm plastic Warm Up Time 5 seconds Automatic Power Down After 90 seconds of inactivity A2 Optical Specifications Light UV LED Blue LED Green LED Source Excitation 365 395 nm 475 15 nm 525 20 nm Optics Emission gt 430 nm 515 20 nm gt 570 nm Optics Limit of gt 50 pM lt 200 pM Detection Fluorescein Rhodamine Picofluor User s Manual 19 Appendix B Internal Datalogging B1 Shipping Checklist Instruments with internal data logging will also receive in their shipment e Interface cable e Spreadsheet Interface Software 1 CD Both of these items are necessary for retrieving the stored data in the Picofluor B2 Hardware Requirements e PC with Windows 95 or later e Ma Excel 5 0 or later e Atleast 1 available serial port B3 Installation Exit all Windows programs 2 Insert CD and run the setup program 3 The setup wizard will install the necessary2. Status Stop lt ENT gt to toggle Download data 5X lt ENT gt to start Erase Data 5X lt ENT gt to start lt ENT gt Y Status Logging lt ENT gt to toggle Download data All downloaded Erase Data All data erased If there is no data logged this screen will appear Data logger has no valid data Picofluor User s Manual 3 2 Instrument Power Up To turn on the Picofluor press the lt ON OFF gt button The instrument takes 5 seconds to warm up After the warm up the Picofluor is ready for operation 3 3 Fluorescence Channels Choose the appropriate channel for your analysis To do this press the lt A B gt button to toggle between the 2 channels The activated channel will be displayed in the lower left corner of the Home screen Model 8000 003 UV Ultra Violet excitation Blue Blue LED excitation Model 8000 004 RHOD Green LED excitation Blue Blue LED excitation See Appendix A for Optical Wavelength Specifications 3 4 Calibration Standard Value Before performing a calibration assign a numeric value between and 999 for your standard solution 1 Press the lt STD VAL gt button 2 Use the up and down arrows to adjust the standard value Holding this button down will activate faster scrolling Picofluor User s Manual 9 3 When finished Press lt ESC gt or lt ENT gt to accept the value an
3. files When the setup is complete an icon named Spreadsheet Interface Software will be found in the Programs menu 4 Restart your computer B4 Connecting 1 Using the provided cable connect the 9 pin adapter of the cable into the available serial port of your computer 2 Plug the opposite end of the cable into the base of the Picofluor 3 Open the Spreadsheet Interface Software Picofluor User s Manual 20 4 Click on the box to the right of the COM port icon to select the appropriate COM port This is usually COM port 1 5 Click Start The program will find and open MS Excel and should be ready for data transfer The boxes left of the COM port and MS Excel should both be green 6 Follow the directions from Section 3 7 for collecting and downloading data from the Picofluor Data will automatically appear in the excel spreadsheet BE SURE to save this data BEFORE closing the software B5 Troubleshooting Difficulties can arise when parameters are set incorrectly or connections with the cable are not tight enough Here are some common problems 1 Box to the left of the COM port is red This means that the COM port is not available Causes a Another instrument or program such as palm pilot hot sync could be occupying the port making it unavailable Make sure to close all programs of this type before downloading data b The port selected is incorrect Follow step 4 of
4. 15 5 Warranty 5 1 Terms Turner Designs warrants the Picofluor Fluorometer and accessories to be free from defects in materials and workmanship under normal use and service for a period of one year from the time of initial purchase with the following restrictions 1 The instrument and accessories must be installed powered and operated in compliance with the directions in this Picofluor User s Manual and directions accompanying the accessories 2 Damage resulting from measurement of samples found to be incompatible with the materials used in the sample system is not covered 3 Damage resulting from contact with corrosive materials or atmosphere is not covered 4 Damage from salts proteins and other moderately corrosive materials that are not promptly removed from the instrument are not covered 5 Damage caused by modification of the instrument by the customer is not covered Picofluor User s Manual 16 5 2 Warranty Service To obtain service during the warranty period the owner shall take the following steps 1 Write or call the Turner Designs Customer Service or Applications Departments and describe as precisely as possible the nature of the problem 2 Make minor adjustments or tests as suggested by Turner Designs personnel 3 If proper performance is not obtained ship the instrument prepaid to Turner Designs The instrument will be repaired and returned free of charge for all
5. TURNER DESIGNS 845 W Maude Avenue Sunnyvale CA 5 408 749 0994 FAX 408 749 0998 www turnerdesigns com Picofluor Handheld Fluorometer User s Manual Dated 03 19 2003 Version 1 2 P N 998 0853 Table of Contents 1 Introduction LL 6 i usteet deeg A 1 2 Inspection and Setup 4 1 3 General Information and Precautions 5 2 Quick View Diagrams e 6 3 Instrument Parameters 3 1 Firmware Flowchart ees H 3 2 Instrument Power Up 9 3 3 Fluorescence 661 9 3 4 Calibration Standard Value pp 9 3 5 Calbranon 10 3 6 Sample Analysis pp 10 3 7 Internal Data Logging 1121 11 3 7 1 Activate Data Logging 11 3 7 2 Download Data 11 3 7 3 6 11 3 8 Diagnostic Information pp 12 4 General Considerations for Analysis 4 1 Handling Samples 13 4 2 Linear Range and Quenching 14 4 3 Temperature ConsiderationS pp 15 4 4 Positioning Samples 15 4 5 Data Quality RN 15 5 Warranty Jel KEE 16 5 2 Warranty Service pp 17 5 3 Out of Warranty SerVice ppp 18 Picofluor User s Manual Appendices A Instrument Specifications Al General 010010 19 A2 Optical 010 19 B Internal Data Logging B1 Shipping Checklist pp 20 B2 Hardware Requirements pp 20 B3 diss 20 B4 Connecting 20 B5 Troubleshooting ppp 21 Picofluor User s Manual 1 Introduction 1 1 Description The Picofluor is a
6. any air bubbles in your sample They can significantly affect the fluorescent reading Picofluor User s Manual 5 2 Quick View Diagrams Picofluor User s Manual 3 Instrument Parameters 3 1 Firmware Flowchart Indicates which channel is active Turner Designs TD HHIB 0500 5 5 second warm up Home Screen XXX X a A lt 4 _ Indicates activity of Fluorescence reading IDL l Press lt A B gt to toggle between XXX X A and XXX X B Press lt STD VAL gt Cal Val XXX X lt UP DOWN gt A Press lt CAL gt Press lt READ gt Calib A READING lt ENT gt to start A AA Insert Blank WAIT XXX X then press lt ENT gt A y Reading Blank XXX X XX X A Insert Cal Soln then press lt ENT gt Press lt DIAG gt y then lt ENT gt to Reading Cal Soln toggle between XX X Logging Points Left XXX Calibration Completed lt ENT gt SS If lt ENT gt is not pressed within 5 seconds this screen will appear Abort Cal lt UP gt Yes lt DOWN gt No FS Blk X XX FS STD XX XX Picofluor User s Manual i Press lt DATA gt Datalogger lt DATA gt to toggle v Press lt DATA gt 1X v Press lt DATA gt 2X l Press lt DATA gt 3X
7. asing of data 3 7 1 Activate Data Logging 1 Press the lt DATA gt button 2 times Press lt ENT gt to toggle between logging and stop Press lt ESC gt when finished to return to the Home screen 3 7 2 Download Data 1 LA Connect the Picofluor to the serial port of your PC using the provided interface cable Open the Spreadsheet Interface Software See Appendix B for computer requirements and installation Press the lt DATA gt button 3 times Press lt ENT gt 5 times to start the data download Press lt ESC gt when finished to return to the Home screen 3 7 3 Erase Data 1 Press the lt DATA gt button 4 times Press lt ENT gt 5 times to erase all logged data Press lt ESC gt when finished to return to the Home screen Picofluor User s Manual 11 3 8 Diagnostic Information 1 2 Press lt DIAG gt to access the diagnostic screens The first screen shows the number of data points available for internal data logging Press lt ENT gt to toggle to the FS Full Scale values from the calibration blank and standard Press lt ESC gt when finished to return to the Home screen Picofluor User s Manual 12 4 General Considerations for Analysis 4 1 Handling Samples 1 Take care not to spill samples into the sample chamber Promptly wipe any spills The Picofluor is very sensitive and even small amounts of material from a previous sample may re
8. connecting to choose another COM port 2 All lights are green but no data transferred even though the instrument says All data downloaded a The connection between the instrument and the computer is bad Check and tighten the cable connections Make sure both ends of the cable are plugged in tightly Picofluor User s Manual 21
9. customers in the contiguous continental United States For customers outside of the contiguous con tinental United States and who have purchased our equipment from one of our authorized distributors contact the distributor If you have purchased direct contact Turner Designs We will repair the instrument at no charge but we will not pay for shipment documentation etc These charges will be billed at cost NOTE Under no conditions should the instrument or accessories be returned without notice Prior correspondence is needed a To ensure that the problem is not a trivial one easily handled in your laboratory with consequent savings to everyone b To specifically determine the nature of the problem so that repair can be rapid with particular attention paid to the defect you have noted Picofluor User s Manual 17 5 3 Out of Warranty Service Proceed exactly as for Warranty Service above If our service department can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a basis of time and materials A complete statement of time spent and materials used will be supplied Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs 845 W Maude Ave Sunnyvale CA 94085 Picofluor User s Manual 18 Appendix A Instrument Specifications Al General Specifications
10. d return to the Home Screen 3 5 Calibration We recommend that you always calibrate before performing any sample analysis 1 2 3 Press the lt CAL gt button Press lt ENT gt to start the calibration Insert your blank and press lt ENT gt The Picofluor will average the blank fluorescence for 10 seconds Insert the calibration standard and press lt ENT gt The Picofluor will average the standard fluorescence for 10 seconds Press lt ENT gt when the calibration is complete to accept the calibration If lt ENT gt is not pressed within 10 seconds you will be asked if you want to abort the calibration Press the up or down arrow to abort or accept the calibration respectively If at anytime during steps 1 4 you want to stop the calibration press lt ESC gt This will return you to the Home screen and default back to the previous calibration 3 6 Sample Analysis 1 2 Insert your sample Press either lt READ gt button The instrument will autorange then measure and average the fluorescence signal over a 5 second interval The result will be displayed at the top and center of the Home screen Picofluor User s Manual 10 4 The top left corner will display WAIT for 5 seconds Once WAIT disappears another sample reading can be performed 3 7 Internal Data Logging IDL Your Picofluor can log up to 1000 data points The DATA screens control logging downloading and er
11. dual channel fluorometer designed for quick easy and accurate fluorescence measurements When properly calibrated with a known standard the Picofluor can be used to determine concentrations of unknown samples 1 2 Inspection and Setup 1 2 1 Inspection Upon receiving your instrument please inspect everything carefully and make sure all accessories are present All shipments include The Picofluor The User s Manual 4 AAA batteries Internal Data Logging IDL package e PC interface cable e Spreadsheet Interface Software 1CD 1 2 2 Setup Before the Picofluor can be used the supplied batteries must be installed 1 On the back of the instrument loosen the screw and remove the battery panel see Section 2 for diagram 2 Install the 4 AAA batteries into the appropriate spaces 3 Replace the battery panel and tighten the screw The panel has an o ring which creates a watertight seal The battery panel may be difficult to install if there is no lubrication on Picofluor User s Manual 4 the o ring Use a silicon based grease to lightly lubricate the o ring if necessary 1 3 General Information and Precautions e The sample compartment cannot accept glass or quartz cuvettes but readily accepts methacrylate or polystyrene cuvettes e Aminimum volume 01 2 mL ina 10 10mm cuvette is required for best results e A minimum volume of 75uL in a Minicell adaptor is required e Avoid having
12. ncentrations are increasing This effect is known as signal quenching Picofluor User s Manual 14 4 3 4 4 5 Linearity may be checked by diluting a sample 1 or some other convenient ratio be sure to use an appropriate blank for the dilutions If the sample is within the linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the sample is beyond the linear range of your fluorophore Temperature Considerations Fluorescence is temperature sensitive As the temperature of the sample increases the fluorescence decreases For greatest accuracy read the blank standard and samples at the same temperature Positioning Samples For low concentration samples cuvettes often will give slightly different measurements depending upon their orientation in the sample compartment This is due to defects in the shape of the cuvette that are not visible to the human eye We recommend that the cuvette be marked at the top and positioned in the sample compartment the same way each time to minimize error Data Quality The Picofluor is only as accurate as the standards that are used to calibrate it This is why it is important to take care when preparing standards samples and blank One should follow good laboratory practices when preparing all solutions and reagents Picofluor User s Manual
13. sult in errors Use a clean cuvette for each reading Thorough and proper cleaning of cuvettes between sample readings is essential and is especially important if you are using the same cuvette for samples and the blank Fill the cuvette at least 50 full significant error will result if it is not filled enough The cuvette MUST BE CLEAN AND DRY on the outside when taking readings Moisture and condensation on the outside can result in error Minute bubbles in samples will cause errors in the readings Take care not to introduce bubbles into samples Particular care must be taken with the Minicell adaptor Slight tapping on the outside cuvette wall will often help dissipate bubbles Picofluor User s Manual 13 4 2 Linear Range and Quenching The linear range is the concentration range in which the readout of the Picofluor is directly proportional to the concentration of the fluorophore The linear range begins with the smallest detectable concentration and spans to an upper limit concentration that is dependent upon the properties of the fluorescent material the filters used and the path length See the diagram below Fluorescence Fluorophore conc A nonlinear relationship is seen at higher concentrations where the fluorescence signal increases at decreasing rates in comparison to the change in concentration At even higher concentrations readings will begin to decrease even though the sample co
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