Project Planning Document and Work Plan State of Florida
Contents
1. Kilroy Sensor Detection Limit Accuracy Temperature 55 to 150 C NA Depth 0 30 psi NA Flow Speed NA NA Flow Direction NA NA YSI Probe Detection Limit Accuracy Temperature 5 to 50 C 5 to 35 C 1 35 50 C 5 DO 0 50 mg L 0 200 1 of reading or 0 1 mg L whichever is greater 200 to 500 5 of reading pH 0 14 units 0 2 pH Turbidity 0 4000 FNU 0 999 FNU 0 3 FNU or 2 of reading whichever is greater 1000 4000 FNU 5 of reading Chlorophyll 0 400 ug L R gt 999 Blue Green Algae 0 280 ug L or 0 to R gt 999 PE 100 RFU Conductivity 0 200 mS cm 5 of reading ORP 999 999 mV 20 mV fDOM 0 300 ppb R gt 999 Salinity 0 70 ppt 1 of reading Nu Lab Sensor Detection Limit Sensitivity NO3 N 0 5 mg L 003 PO4 P 0 8 mg L 003 Data Quality Checks Each deployment site will be visited no less than once per month at which time YSI measurements will be verified using a YSI handheld sonde identical to the deployed instrument measuring salinity conductivity temperature dissolved oxygen pH ORP fDOM total algae and turbidity The handheld sonde will be calibrated and verified with applicable portions of DEP SOPs FT 1000 field testing general FT 1100 pH FT 1300 salinity FT 1500 DO and FT 1900 field continuous monitoring prior to the site visit and then verified after the m2. CRCA Project Planning Document and Work Plan State of Florida DEP Agreement Number S0769 Ocean Research amp Conservation Association 1 of 21 Project Background The Indian River Lagoon IRL is a diverse shallow water estuary stretching 156 miles along 40 of Florida s east coast The lagoon is an important economic resource to the state providing a total estimated annual economic value of 3 7 billion supporting 15 000 full and part time jobs and providing recreational opportunities for 11 million people annually In spring 2011 an algal super bloom occurred throughout most of the Brevard County and Indian River portion of the lagoon Approximately 47 000 acres of seagrasses were lost a reduction of about 60 valued at 235 million to 470 million in commercial and recreational fisheries losses Seagrasses provide food shelter and nursery areas for marine life in the lagoon but seagrasses need sunlight to grow In the southern portion of the IRL freshwater discharges from Lake Okeechobee and surrounding basins resulted in a 100 loss of oyster restoration areas in the St Lucie Estuary Throughout the lagoon the discharges prohibited light from reaching seagrass beds resulting in loss added nutrients to an already nutrient saturated system fueling large algae blooms and carried a variety of other foreign materials of aquatic and human health concern Many critical water quality parameters effecting pollution measurements chan
3. clean and dry or pre rinsed calibration cup making sure the sensor s glass bulb is in solution by at least lcm Allow at least 1 minute for temperature equilibration before proceeding 3 7 3 Calibrate gt pH or pH ORP gt pH Ocean Research amp Conservation Association 10 of 21 3 7 6 3 7 7 3 7 8 3 7 9 3 7 10 3 7 11 3 7 12 3 7 13 3 7 14 Select the number of points desired for the calibration Enter the value s of the pH buffer s that will be used for the calibration Observe the temperature reading above the standard value The actual pH value of all buffers varies with temperature Enter the correct value from the bottle label for your calibration temperature for maximum accuracy For example the pH of one manufacturer s pH 7 Buffer is 6 994 at 25 C but 7 012 at 20 C Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Confirm that the Pending data value is close to the Set Point value Click Proceed and wait for the software to prompt you to move the sensor to the next standard solution Rinse the sensor in deionized water If possible rinse sensor with extra pH buffer solution to avoid dilution of the next buffer solution Pour the correct amount of an additional pH buffer standard into a clean dry or pre rinsed calibration cu
4. gt 0 999 for serial dilution of 300 ppb QS solution ORP 20 mV in Redox standard solution pH 0 1 pH units within 10 C of calibration temp 2 pH units for entire temp range Blue Green Algae Phycoerythrin Linearity R gt 0 999 for serial dilution of Rhodamine WT solution from 0 to 280 ug mL BGA PE equivalents Chlorophyll Linearity R gt 0 999 for serial dilution of Rhodamine WT solution from 0 to 400 ug mL Chl a equivalents Turbidity 0 to 999 FNU 0 3 FNU or 2 of reading 1000 to 4000 FNU 5 of reading Salinity calculated from conductivity and temperature 3 4 Conductivity Ocean Research amp Conservation Association 1 0 of reading or 0 1 ppt 9 of 21 3 4 1 3 4 2 3 4 3 3 4 4 3 4 5 3 4 6 3 4 7 3 4 8 3 4 9 3 4 10 3 4 11 3 4 12 This procedure calibrates conductivity non linear function nLF conductivity specific conductance salinity and total dissolved solids Clean with soft brush Place the correct amount of conductivity standard into a clean and dry or pre rinsed calibration cup ORCA s in house NIST traceable Conductivity Temperature and Depth sensor Smart Conductivity Temperature and Pressure V1 15 SN 4893 CTD 1998 Applied Microsystems Ltd serves as a baseline for measuring calibration standard Carefully immerse the probe end of the sonde into the solution making sure the standard is above the vent holes on th
5. required 865 1 0 003440 004 mg L Chlorophyll a SM10200H 1 1mg m3 Grab sampling will be performed in accordance with DEP SOP 001 01 and PACE Analytical Services requirements and in compliance with Table FS 1000 4 of DEP SOP FS 1000 the following sample preservation will be conducted within 15 minutes of collection filtration for ortho phosphate acidification for nitrate and nitrite unless analysis will occur within 48 hours of collection thermal preservation immerse samples in ice for ortho phosphate nitrogen and chlorophyll a In accordance with FQ1230 field blanks will be collected at a frequency of 5 or one blank for every 20 samples One to two of the required 15 field blanks with be obtained during each monthly maintenance cycle for the duration of the project Lab results will be compared to instrument readings by ORCA engineers John Taylor Morgan Marmitt and or Billy Wells Values from lab results will be added to the ORCA database for reference At the end of the project ORCA engineers will compare paired lab and instrument results using paired t tests and comparative plots to determine the comparability of the results Readings from each instrument will occur every 4 hours In the event the average difference of 12 consecutive readings from a sensor exhibits a 25 drift from the previous 12 consecutive readings that cannot be explained by know environmental changes it will be rechecked for proper calibration in situ by compar
6. the probe end of the sonde into the water Calibrate gt Turbidity gt Turbidity FNU Click 3 Point for the Calibration Points Enter 0 FNU for first standard value 124 FNU for second standard value and 1010 for the third value 0 must be calibrated first Start Calibration Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point If the temperature of your field site is substantially different from the lab temperature allow the sensor to sample for 3 5 minutes at each calibration point before accepting it This step ensures the best possible temperature compensation when deployed Next place the sensors in the second calibration standard Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Ocean Research amp Conservation Association 12 of 21 3 9 11 Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor Click Complete View the Calibration Summary screen and QC score 3 9 12 Click Exi
7. 00 1 Field Testing Acceptance Criteria Acceptance Criteria Acceptance Criteria a program criteria 0 5 C of NIST traceable value with correction factors Verification over range of applicable values FT 0 1 10 NTU 10 of standard value 11 40 NTU 8 of standard value 41 100 NTU 6 5 of standard value gt 100 NTU 5 of standard value Data Access Data from the Kilroy Monitoring Systems will be reviewed for accuracy and quality of data on a daily basis by ORCA engineers John Taylor Morgan Marmitt and Billy Wells During monthly maintenance cycles ORCA s database management will review the data stream with field data to ensure accurate data is transmitted from deployed sensors Real time data from the most recent measurement and a historical graph of the previous 3 days can be accessed through ORCA s home page at www teamorca org Historical data ranging from installation to current date can be viewed by requesting access to ORCA s database Access is granted through log in to ORCA s internal website with user name and password Viewing of data tables or exporting of csv files are accessible through this site Suspect data will be marked as questionable until confirmed valid Data determined to be not viable will be removed from the dataset Kilroy System Calibration Calibration of instruments will be performed in laboratory conditions employing methods outlined by the manufacturer and in accordance with applicable por
8. 3 10 25 ug L l or 2 point This procedure will zero your fluorescence sensor and use the default sensitivity for calculation of phycocyanin containing BGA in ug L allowing quick and easy fluorescence measurements that are only semi quantitative with regard to BGA PC However the readings will reflect changes in BGA PC from site to site or over time at a single site 3 10 26 Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water 3 10 27 Calibrate gt BGA PC Chlor gt BGA pg L gt 1 or 2 point calibration 3 10 28 Enter 0 for first standard value and 16 for second standard value 3 10 29 Start Calibration Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point 3 10 30 Next place the sensors in the Rhodamine WT standard Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point 3 10 31 Click Complete View the Calibration Summary screen and QC score Click Exi
9. No 7 Taylor Creek 27 466811 80 326105 Yes No No 8 Queen s Cove 27 517234 80 325823 Yes No No 9 Queen s Cove N 27 533574 80 322024 Yes No No 10 Vero Shores 27 563693 80 359567 Yes No No 11 Main Relief Canal 27 650523 80 375658 Yes No No 12 Grand Harbor 27 678010 80 386750 Yes No No 13 Pine Island 27 752312 80 411712 No Yes Yes 14 Sebastian River 27 830499 80 503874 Yes No No 15 C53 27 834865 80 513469 Yes No No 16 Goat Creek 27 968335 80 544755 No Yes Yes 17 Turkey Creek 28 032744 80 580925 Yes No Yes 18 Crane Creek 28 076103 80 599700 Yes No No 19 Eau Gallie River 28 124573 80 623762 Yes No No 20 Grand Canal N 28 211031 80 617468 Yes No No 21 Sykes Creek 28 316768 80 672030 No Yes No 22 Barge Canal 28 406353 80 719578 Yes No No 23 Addison Creek 28 545222 80 787710 Yes No No 24 Banana Creek W 28 593821 80 722643 Yes No No 25 Turnbull Creek 28 798334 80 850268 No Yes Yes Ocean Research amp Conservation Association 3 of 21 Map of Installation Sites Kilroy Systems Locations Rees x G Kilroy Monitoring Systems Banana ie West End Addison Creeki tusville Barge Canali TAN Siecle F Chand Canal North e G Eau Gallie River Crane cat Goat ereek Falls Bray conten d FIE l pS 0 Beach WET Relief eas Send reboot Y N Gu 2 Cove Queens Gove Northy Moores Cree glaylor Creek CORN Bluefield Preserve hE OFS Willoughby CreerSouth Fork Palm City Bridge lt 4 a i f A Sewells Poin
10. Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point 3 10 18 Next place the sensors in the Rhodamine WT standard Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles 3 10 19 When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point Ocean Research amp Conservation Association 13 of 21 3 10 20 Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu 3 10 21 Rinse the sonde in tap or purified water and dry the sonde 3 10 22 Blue green Algae Phycocyanin This procedure calibrates BGA RFU or BGA ug L 3 10 23 If the user has both units selected then this procedure must be performed twice once for each unit to completely calibrate the parameter 3 10 24 For the 2 point calibration one of the standards must be clear water 0 ug L and this standard must be calibrated first The other standard should be in the range of the suspected BGA PC content at the environmental site We will use a 625 ug L Rhodamine WT dye solution for detailed instructions see section on calibrating for chlorophyll and the solution is used in the calibration steps below
11. e conductivity sensor Gently rotate and or move the sonde up and down to remove any bubbles from the conductivity cell Allow at least one minute for temperature equilibration before proceeding In the Calibrate menu select Conductivity and then a second menu will offer the options of calibrating conductivity nLF conductivity specific conductance or salinity Calibrating any one option automatically calibrates the other parameters After selecting the option of choice specific conductance is recommended enter the value of the standard used during calibration Be certain that the units are correct and match the units displayed in the second window at the top of the menu Click Start Calibration Observe the readings under Current and Pending data points and when they are stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point If the data do not stabilize after 40 seconds gently rotate the sonde or remove reinstall the cal cup to make sure there are no air bubbles in the conductivity cell Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Rinse the sonde and sensor s in tap or purified water and dry 3 5 Dissolved Oxygen Sensor 3 5 1 3 5 2 3 5 3 3 5 4 3 5 5 3 5 6 3 5 7 3 5 8 These directions are for a 1 point calibration For a 2 poin
12. ecessary 6 0 Data Quality 6 1 Device Operation The Kilroy Monitoring System is comprised of an ORCA designed and constructed monitoring instrument that measures flow speed flow direction water depth and water temperature The Kilroy Monitoring system is integrated with a YSI EXO2 sonde with probes measuring ph ORP Conductivity Temperature Optical DO Turbidity total algae that includes chlorophyll and blue green algae sensors and f DOM Additionally the Kilroy is integrated with a NuLab nutrient monitoring system that monitors nitrate nitrite and ortho phosphate by utilizing wet chemistry which is not affected by bromides water color changes and other contaminants that affect optical nutrient sensors Ocean Research amp Conservation Association 20 of 21 The integrated package is coupled to an ORCA designed and constructed communications system that reports to the ORCA designed database at 4 hour intervals The complete system is solar powered and utilizes a battery backup for times when sunlight is not available Each deployment site will be visited no less than once per month at which time YSI measurements will be verified using a YSI handheld sonde identical to the deployed instrument measuring salinity conductivity temperature dissolved oxygen pH ORP fDOM total algae and turbidity The handheld sonde will be calibrated and verified with applicable portions of DEP SOPs FT 1000 field testing general FT 1100 pH FT 1300 salini
13. econds click Apply to accept this calibration point 3 11 16 Remove the central wiper from the EXO2 sonde before proceeding to the next step 3 11 17 Next place the sensors in the correct amount of 300 ug L quinine sulfate standard in the calibration cup Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing verify that no air bubbles reside on the sensing face of the sensor If there are bubbles gently shake or move the sensor to dislodge When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point 3 11 18 Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu 3 11 19 RFU 1 or 2 point 3 11 20 Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water 3 11 21 Calibrate gt fDOM gt RFU gt 1 or 2 point calibration 3 11 22 Enter 0 for first standard value and 100 RFU for second standard value 3 11 23Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point 3 11 24 Remove the central wiper from the EXO2 sonde before proceeding to t
14. erface 3 Allow at least 1 hour for reagents to equilibrate before testing 4 DONOT leave the system running without physically monitoring the first measurement cycle in case of a leak or pinched line Procedure 1 Initial Reagent Blank As with any reagent change a reagent blank needs to be re calculated to compensate for any nutrients potentially present within the system The inlet line must be placed in D I de ionized water for a proper blank to be obtained A standard 70 micron filter needs to be installed as well in the event a contaminant gets into the sample as well as loading the mechanics with equivalent resistance to be seen in the field Once configured run a 5 measurement reagent blank Run both Prime and Inlet Flush macros to ensure no air is trapped in the lines Enter values in the table below after completion the dd KION command will display the results Ifthe OBS is out of range then re activate the cadmium column and repeat the test Initials Date Sample OBS 0 03 0 04 Absorption lt 0 008 1 2 3 4 5 Average Abs Deviation D I Creation Date Note 1 DO NOT wipe the records on the KION resulting from the testing as these will be uploaded to the database for recording Procedure 2 Known Test Standard Once a reagent blank is correctly completed the instrument is to be run overnight in order to stress both the mechanics and to monitor measu
15. esults obtained from more typical methods Comparable data from this project will allow counties cities and agencies to assess compliance with established TMDLs and make informed decisions for meeting compliance and other matters that affect the waters of the IRL During the process the Grantee will employ services of a statistician to analyze data obtained and create analysis plots for distribution from Grantee s website The Grantee will obtain permission and or permits for pilings needed to mount the monitoring systems Upon Agreement execution the Grantee will order components to assemble 12 of the 25 required systems for the project Assembly of the first 12 systems will be completed no later than April 2015 Assembly of the remaining 13 systems will run concurrent with the deployment of the initial 12 systems Deployment of all systems will be completed by June 2015 For the duration of the project the Grantee s engineers will check calibration and clean each system at two 2 week intervals At each two 2 week interval but no longer than a four 4 week interval reagents for the NuLab nutrient monitoring system will be checked and replenished as necessary and the waste container emptied and water disposed of as required Installation Plan Method of Installation The above water portion of the Kilroy Monitoring System is comprised of the communications wet chemistry and solar power attached to a 2 x2 fiberglass mounting pole with bracket
16. ge during daily cycles reducing the usefulness of sporadic sampling Kilroy Monitoring Stations can be installed to measure flow speed flow direction water temperature wave conditions water depth and can accommodate additional instruments to monitor other water quality parameters almost constantly Kilroys communicate in near real time to a land based database with data available to the public via Internet access Stationing Kilroys at key discharge points into the IRL will allow us to identify discharge sites that are contributing the most pollution and prioritize those for mitigation Project Description Working with local agencies and non governmental organizations NGOs 25 discharge sites will be selected along the IRL from Volusia County to Martin County Monitoring instruments will be placed in these discharge sites to monitor water parameters and nutrient content from these discharge sites into the IRL The Grantee will consult with agencies and NGOs in the counties along the IRL to identify discharge sites of interest in each respective area The Grantee will install 25 Kilroy monitoring systems to monitor for flow speed flow direction water temperature water depth salinity turbidity pH dissolved oxygen chlorophyll a blue green algae and oxygen reduction potential Monitoring will also be conducted for nitrates and phosphates Data from these sondes will be compared with independent sonde and laboratory data to show comparability to r
17. he next step 3 11 25 Next place the sensors in the correct amount of 300 ug L quinine sulfate standard in the calibration cup Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing verify that no air bubbles reside on the sensing face of the sensor If there are bubbles gently shake or move the sensor to dislodge When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point 3 11 26 Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu 3 11 27 Rinse the sonde in tap or purified water and dry the sonde Discard the used standard 4 0 NuLAB 4 1 NuLab Nitrogen Procedure Instrument Identification NuLAB Specific Serial Number Wavelength nm Master Macro Revision ORCA Specific NuLAB N N Device ID database ref KION S N KION F W Ocean Research amp Conservation Association 16 of 21 Baseline Reagent Configuration Reagent Database Reference ID NEDA Sulphanilamide Imidazole OBS N N Standard OBS Concentration mg l Notes 1 DONOT use reagents that have EOL end of life dates that are beyond date of first maintenance cycle 2 Register reagents being used in database before deployment via engineering web int
18. ic item AA35651K7 3 11 6 Weigh 0 100 g of solid quinine sulfate dihydrate and quantitatively transfer the solid to a 100 mL volumetric flask Dissolve the solid in about 50 mL of 0 05 M 0 1 N sulfuric acid H dilute the solution to the mark of the volumetric flask with additional 0 05 M sulfuric acid and mix well by repeated inversion This solution is 1000 ppm in quinine sulfate 0 1 3 11 7 Transfer 0 3 mL of the 1000 ppm solution to a 1000 mL volumetric and then fill the flask to the top graduation with 0 05 M sulfuric acid Mix well to obtain a solution of 300 ug L 300 QSU or 100 RFU 3 11 8 Store the concentrated standard solution in a darkened glass bottle in a refrigerator to retard decomposition The dilute standard prepared in the previous step should be used within 5 days of preparation and should be discarded immediately after exposure to EXO s metal components Degradation of quinine fluorescence by copper and chloride Exposure of the quinine sulfate solution to any copper based component of the EXO sonde and sensors primarily the wiper assembly will begin to degrade the solution significantly within minutes Quinine fluorescence is also degraded by the presence of chloride or halide ions found in estuarine or seawater conductivity standards and Zobell solution Thus clean your sensors thoroughly and perform your calibration as quickly as possible on immersion of the sensors into the quinine sulfate solution Discard the
19. ill be uploaded to the database for recording Procedure 2 Known Test Standard Once a reagent blank is correctly completed the instrument is to be run overnight in order to stress both the mechanics and to monitor measurement stability Replace the D I with a premixed standard Connect sample chamber and pump system to allow the chamber to be operational for this test The sample chambers inlet outlet lines need to be placed in a bucket with some clean water does not have to be D L Disable all replicates and use the alt sample table hourly sampling Initials Date Test Standard Concentration mg l Test Standard Database ID Sample OBS 0 03 0 04 Conc mg l 1 2 3 4 5 6 7 8 Average Pump Current Dropped OBS bad count good count Ocean Research amp Conservation Association 19 of 21 Post Operation If the unit is to be stored prior to deployment drop all the lines into D I and prime the system several times to wash all reagent from both lines and valve 5 0 Field Maintenance and Operation 5 1 General Procedures 5 1 1 Each mooring is to be visited every 4 weeks Each station is equipped with a call button which allows the field crew to register the visit with the database This allows for consistent record keeping and assists in locating invalid data resulting from any maintenance operations Each visit will require device specific operations listed belo
20. ison with a calibrated instrument If found to be out of calibration the instrument will be removed from the field and recalibrated before being redeployed or will be replaced by a calibrated instrument provided an instrument is available In the event a grab sample differs from the in situ instrument reading by greater than 25 an additional grab sample will be obtained for verification of the results If 2 consecutive grab samples differ from the instrument measurement by more than 25 the instrument will be checked for malfunction and removed from the field for repairs if confirmed At no less than 6 month intervals instruments will be removed from the field for laboratory controlled calibration checks These checks will be conducted per applicable portions of DEP SOPs FT 1000 FT 1100 pH FT 1300 salinity FT 1500 DO and FT1900 continuous monitoring using acceptance criteria in Table FT 1000 1 see below Calibrations will be checked for instruments removed from the field for laboratory controlled calibration prior to re calibration and readings will be recorded in the ORCA database before and after laboratory calibration Due to the harsh conditions instruments may from time to time fail and require repair or replacement All efforts will be made to repair instruments in a timely manner and when possible a replacement instrument will be deployed until repairs can be completed Ocean Research amp Conservation Association 6of21 Table FT 10
21. ndard Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu Rinse the sonde in tap or purified water and dry the sonde F ffect of temperature on ORP The oxidation reduction potential value shows an inverse relationship with temperature This effect must be accounted for when calibrating the EXO ORP sensor with Zobell solution Enter the mV value from the table below that corresponds to the temperature of the standard Ocean Research amp Conservation Association 11 of 21 3 9 EXO2 Turbidity Sensor 3 9 1 3 9 2 3 9 5 3 9 6 3 9 10 Before calibrating be certain that the probe is clean and free of debris Use a clean spare sonde guard For proper calibration you must use a NIST Traceable standard YSI offers different standards designed to specifically calibration the turbidity standard O FNU EXO YSI 6 Series 1 gallon LANAT ANN OU 400 In addition to the standards available through YSI there is a 100 NTU standard by AMCO AEFPA Item No 8021 and a 1 000 NTU standard by Ricca Chemical Cat NO 8825 16 Due to the non linear function of the sensor calibration ranges may be limited point 2 point 3 point I FNU i5 199FNU 200 42 O FNU lor NTU i lor NTU i for NTU For a 3 point calibration Pour the correct amount of 0 NTU standard clear deionized or distilled water into the calibration cup Immerse
22. nsors The unit is then sealed and an internal positive pressure is applied to it before being submerged for leak testing After confirming no leaks then circuit board is reinstalled and reconnected to the sensors 2 3 Pressure Calibration 2 3 1 Once the Kilroy is reassembled a recalibration of the pressure sensor is done The Kilroy is secured to the test equipment and the ambient pressure is logged Measurements of the pressure sensor s output are polled and recorded at known levels of head pressure These are then plotted and compared to what the head pressure and the atmospheric pressure are exerting and a linear relationship is plotted From this linear plot an offset and gain value are recorded to be written to the Kilroy software package Once the Kilroy is reassembled a recalibration of the pressure sensor is done The Kilroy is secured to the test equipment and the ambient pressure is logged A test cylinder is filled with water and the control board polls both Kilroy s pressure gauge output and the NIST pressure gauge The two values are recorded and the cylinder is gradually drained of water while the two gauges continue to record measurements The two data sets are then plotted and a linear relationship is found between them This provides gain and offset coefficients for pressure which are recorded and then written to the Kilroy 2 4 Temperature Calibration 2 4 1 A temperature bath is prepared at a specified range Using a CTD to monitor the
23. onthly site visits Verifications for pH and salinity will be conducted with standards that bracket the sample readings from the field If post sampling verifications do not meet acceptance criteria listed associated sample data will be qualified with J estimated Any deployed instrument found out of calibration will be returned to the lab for calibration and check before redeployment Calibration checks will be recorded in the ORCA database prior to and after recalibration At each monthly site visit grab samples will be obtained by ORCA team members Billy Wells and Morgan Marmitt Grab samples will be sent overnight to PACE Analytical Services Inc for comparability assessment of Nitrate Nitrite Orthophosphate and Chlorophyll a At each Ocean Research amp Conservation Association 5 of 21 monthly maintenance check reagents for the NuLab nutrient monitoring device will be replenished and a grab sample will be obtained for verification For this project PACE Analytical Services Inc 8 East Tower Circle Ormond Beach FL has been selected State of Florida certification E83079 and PACE Quality Assurance Manual attached PACE method and detection limits for this project as follows Test Description Method MDL PQL Unit Nitrogen Nitrate 300 0 353 2 0 025 0 05 mg L Nitrogen Nitrite 300 0 353 2 0 0250 05 mg L Nitrogen Nitrate Nitrite calculation 300 0 353 2 0 0250 05 mg L Ortho Phosphate field filtration
24. oroughly with de ionized water DIW A 3 ww minimum of three rinses will suffice To avoid diluting calibration standards perform three rinses with the standard to be used after the DIW rinse When using a full sensor load fill the calibration cup to the first line If not using an entire sensor load fill to the second higher line Actual volumes of standard will vary depending on how many and what instruments are being calibrated ensure the sensor head is completely submerged in standard prior to beginning calibration If different types of sensors are being calibrated be sure to do a complete rinse of the cup and sensors to avoid cross contamination of different types of standards Also a dedicated calibration cup and sensor guard is maintained on hand in the lab to ensure a greater degree of cleanliness and accuracy This guard and cup will never be used in the field Basic Calibration in KOR Software Once connected to the YSI host software the user has the option to select what type of instrument is being calibrated Individual instrument calibration procedures are described below Some instruments have one parameter to calibrate while others have more than one Users can also choose to perform 1 2 or 3 point calibrations Again these options are described below for individual sensors Always ensure the inputs for the calibration standards are correct and matching e g microSiemens vs milliSiemens There s also the option to in
25. p and carefully immerse the probe end of the sonde into the solution Allow at least 1 minute for temperature equilibration before proceeding Repeat the calibration procedure and click Apply when the data are stable Rinse the sensor and pour additional pH buffer if necessary Repeat calibration procedure for the third point and click Apply when data are stable Click Complete View the Calibration Summary screen and QC score A green check indicates a successful calibration Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Rinse the sonde and sensors in tap or purified water and dry 3 8 Oxidation Reduction Potential ORP Sensor 3 8 1 3 8 2 3 8 3 3 8 4 3 8 5 3 8 6 3 8 7 3 8 8 ORP calibration standard is made by mixing 125ml of DIW with YSI s 3682 Zobell ORP Calibration Solution This NIST traceable ORP standard is created specifically by YSI to calibrate the EXO s ORP sensor Pour the correct amount of standard with a known oxidation reduction potential value in a clean dry or calibration cup Calibrate gt pH ORP gt ORP mV Start Calibration Observe the readings under Current and Pending data points When stable for 1 min click Apply to accept calibration point Do not leave sensors in Zobell solution for a long time A chemical reaction occurs with the copper on the sonde that will degrade the sonde materials over time Discard the used sta
26. put the type of standard manufacturer of the standard and lot number for tracking purposes As a standard procedure always fill in all available information for each standard used When ready click the Start Calibration button The probes begin to sample and data will be displayed on the screen Clicking the Graph Data button will show current samples as they are taken Initially for all instruments the data will appear unstable and will slowly move to stable Users can also compare the pre cal and post cal values in graph form After confirming that the values are within the acceptable margin of error as listed in chart below click Apply to accept the updated calibration This procedure needs to be repeated for each calibration point and each parameter Click Complete when all points have been calibrated A calibration summary appears with a QC score A green check mark indicates a successful calibration while a red X indicates an improper calibration In the case of an unsuccessful calibration repeat the calibration procedure At the end of every calibration description in this document a sample Calibration Worksheet is provided Sensor Acceptable margin of error Conductivity 0 to 100 55 of reading or 0 0001 mS ch w i g 100 to 200 1 of reading Temperature 5 to 35 0 01 C 35 to 50 C 0 05 C Dissolved Oxygen 0 to 20 mg L 0 1 mg L 20 to 50 mg L 5 of reading fDOM R2
27. rement stability Replace the D I with a premixed standard Connect sample chamber and pump system to allow the chamber to be operational for this test The sample chambers inlet outlet lines need to be placed in a bucket with some clean water does not have to be D L Disable all replicates and use the alt sample table hourly sampling Ocean Research amp Conservation Association 17 of 21 Initials Date Test Standard Concentration mg l Test Standard Database ID Sample OBS 0 03 0 04 Conc mg l 1 2 3 4 5 6 7 8 Average Pump Current Dropped OBS bad count good count Post Operation If the unit is to be stored prior to deployment drop all the lines into D I and prime the system several times to wash all reagent from both lines and valve If storage is longer than 3 days remove the Cadmium column and store in a solution D I plus and Imidazole to avoid damage and the necessity of an additional reactivation 4 1 1 NuLab PO4 Procedure Instrument Identification NuLAB Specific Serial Number Wavelength nm Master Macro Revision ORCA Specific NuLAB PO4 Device ID database ref KION S N KION F W Baseline Reagent Configuration Reagent Database Reference ID Ascorbic Acid Ammonium Molybdate Wash 35 isopropyl OBS PO4 Standard OBS Concentration mg l Notes 5 DO NOT use reagents that ha
28. s attached to facilitate attachment to the piling On some locations the Kilroy device along with the YSI sonde and the water pump for the wet chemistry system is also installed on the 2 x2 fiberglass mounting pole and deployed below the water surface Attachment to the piling is completed by strapping the brackets of the mounting structure to the piling at the upper midpoint and lower portions of the mounting structure On locations where the below water devices are not attached to the piling they are attached to a benthic mounting structure comprised of a 3 foot concrete pyramid with 2 x2 fiberglass mounting structure embedded in the concrete pyramid The instruments are attached to the device and the structure is lowered into the water to rest on the bottom Ocean Research amp Conservation Association 2 of 21 Installation Sites Sites are listed in order of installation Site coordinates are approximate Final coordinates will be noted at installation Site Site Description Latitude Longitude Mounting Piling Permit Structure Needed Required in place 1 Palm City Bridge 27 171312 80 259958 Yes No No 2 C23 27 204825 80 284285 Yes No No 3 North Fork 27 260502 80 330308 Yes No No 4 Willoughby Creek 27 170789 80 202144 Yes No No 5 Sewell s Point 27 186279 80 189168 Yes No No 6 Moore s Creek 27 451736 80 322274 Yes No
29. t Marina W i gt N 60 mi Pilings Installed pilings will be comprised of 6 PVC electrical conduit jetted in Two pilings may be installed in each location needing pilings to accommodate 2 weatherproof boxes housing communications and wet chemistry functions of the Kilroy Monitoring System Kilroy Monitoring System Technology OVERVIEW The Kilroy Monitoring System is comprised of an ORCA designed and constructed monitoring instrument that measures flow speed flow direction water depth and water temperature The Kilroy Monitoring system is integrated with a YSI EXO2 sonde with probes measuring ph ORP Conductivity Temperature Optical DO Turbidity total algae that includes chlorophyll and blue green algae sensors and fDOM Additionally the Kilroy is integrated with a NuLab nutrient monitoring system that monitors nitrate nitrite and ortho phosphate by utilizing wet chemistry which is not affected by bromides water color changes and other contaminants that affect optical nutrient sensors The integrated package is coupled to an ORCA designed and constructed communications system that reports to the ORCA designed database at 4 hour intervals The complete system is solar powered and utilizes a battery backup for times when sunlight is not available Ocean Research amp Conservation Association 4 of 21 Detection Limits and accuracy for each of the sensors used in the Kilroy Monitoring system are as follows
30. t calibration refer to user manual Fill calibration cup with water and saturate with a running air stone for a minimum of 1 hour Place DO sensor and thermistor in saturated water Wait approximately 5 minutes before proceeding to allow the temperature and oxygen pressure to equilibrate Calibrate gt ODO gt ODO sat Enter current barometric pressure in mm of Hg NIST Traceable barometer Inches of Hg x 25 4 25 4 mm of Hg Click 1 Point Calibration and enter the standard value Start Calibration Once data are stable for 1 min click Apply to accept this calibration point For further information refer to Section 5 3 Page 73 of EXO Sonde 2 User Manual 3 6 Depth and Level Sensor 3 6 1 Remove depth sensor from solution in air 3 6 2 Calibrate gt Port D Depth gt Depth or Level 3 6 3 Click 1 Point Calibration Enter 0 3 6 4 Start Calibration After 1 min of stable data click Apply to accept calibration point This process zeros the sensor with regard to current barometric pressure 3 6 5 Click Exit to return to the sensor calibration menu 3 6 6 Ensure the orientation of the sonde remains constant while taking reading 3 7 pH Sensor 3 7 1 Select the 3 point option to calibrate the pH probe using three calibration standards In this procedure the pH sensor is calibrated with a pH 7 buffer and two additional buffers ideally pH buffers of 4 and 10 3 7 2 Pour the correct amount of pH buffer in a
31. t to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu 3 10 32 Rinse the sonde in tap or purified water and dry the sonde 3 10 33 RFU l or 2 point RFU is a percent full scale output it outputs relative fluorescence from 0 100 This calibration procedure is recommended if you are also using grab samples to post calibrate in vivo algae readings 3 10 34 Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water 3 10 35 Calibrate gt BGA PC Chlor gt BGA RFU gt 1 or 2 point calibration 3 10 36 Enter 0 for first standard value and 16 for second standard value 3 10 37 Start Calibration Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point 3 10 38 Next place the sensors in the Rhodamine WT standard Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point 3 10 39 Click Complete View the Calibration Summar
32. t to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu 3 9 13 Rinse the sonde in tap or purified water and dry the sonde 3 10 EXO2 Chl BGA PC Total Algae Sensor There are two different parameters that must be calibrated independently Chlorophyll and BGA PC Total Algae 3 10 1 This procedure calibrates Chlorophyll Relative Fluorescent Unit RFU or Chlorophyll ug L If the user has both units selected then this procedure must be performed twice once for each unit to completely calibrate the parameter 3 10 2 For 2 point calibrations one standard must be clear water 0 ug L and this standard must be calibrated first The other standard is a 625 ug L Rhodamine WT dye solution Detailed instructions on preparation come directly from the EXO2 User Manual 3 10 3 The solution is used in the calibration steps below 3 10 4 ug L l or 2 point This procedure will zero your fluorescence sensor and use the default sensitivity for calculation of chlorophyll concentration in ug L 3 10 5 Pour enough deionized water into the calibration cup to immerse the probe end of the sonde in the water 3 10 6 Calibrate gt BGA PC Chlor gt Chl ug L gt 2 point calibration 3 10 7 Enter 0 for first standard value and 66 for second standard value 3 10 8 Start Calibration Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate
33. temperature the Kilroy is allowed to soak while the heater chiller is allowed to run The computer polls both the CTD and Kilroy for the values of their onboard thermometers these values are then stored for processing After running the desired temperature range the data is plotted and a linear relationship between the two data sets is found The resultant gain and offset values are then used as coefficients for Kilroy s temperature sensor 2 5 Acoustic Verification Test 2 5 1 During construction and at 6 month intervals a representative number of Kilroy device acoustics will be verified by comparison to a calibrated acoustic monitoring device 3 0 YSI EXO2 Multi parameter Sonde 3 1 Basic Calibration Procedures EXO Sonde 2 sensors require periodic calibration to ensure peak performance For calibration all basic steps are the same with some specific variations for individual instruments and standards All EXO Sonde 2 instruments are calibrated after 6 months of continuous deployment or when data is deemed questionable through hand sampling comparisons or erratic unrealistic data samples whichever comes first All EXO2 calibrations produce a Calibration Worksheet This worksheet will be catalogued and Ocean Research amp Conservation Association 8 of 21 maintained by ORCA s engineering department in each instrument s history file and will be readily available upon request 3 2 Set Up The EXO calibration cup should always be rinsed th
34. the wiper to remove any bubbles 3 10 9 When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point 3 10 10 Next place the sensors in the Rhodamine WT standard Click Proceed on the pop up window Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are Stable or data shows no significant change for approximately 40 seconds click Apply to accept this calibration point 3 10 11 Click Complete View the Calibration Summary screen and QC score Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu 3 10 12 Rinse the sonde in tap or purified water and dry the sonde 3 10 13 RFU 1 or 2 point RFU is a percent full scale output it outputs relative fluorescence from 0 100 3 10 14 Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water 3 10 15 In the Calibrate menu select BGA PC Chlor then select Chl RFU Select either a 1 or 2 point calibration 3 10 16 Enter 0 for first standard value and 16 4 for second standard value 3 10 17 Click Start Calibration Observe the readings under Current and Pending data points While stabilizing click the Wipe Sensors button to activate the wiper to remove any bubbles When data are
35. tions of DEP FT guidelines as listed in Data Quality Checks on page 5 of this planning document Calibrations will be recorded on calibration worksheets and calibrations logged into the ORCA database Instrument removed from the field will be checked for calibrations prior to recalibration and values from pre calibration and post calibration checks will be recorded and logged into the ORCA database Handheld calibration checks will be performed using a YSI EXO handheld display attached to a YSI EXO2 sonde The YSI EXO2 sonde used for handheld calibration checks will be calibrated prior to monthly site visits and checked for calibration after site visits are completed Values will be checked at each monthly site visit for conductivity temperature dissolved oxygen pH ORP fDOM total algae and turbidity Values from pre and post calibration checks for the handheld device will be recorded in the ORCA database 1 0 Kilroy System Database 1 1 ORCA maintains a relational database for storing both field measurements and the Kilroy System control and configuration The database will allow for an automated system of checks and balances to help ensure proper operation of the Kilroy System 1 2 Calibration Constants 1 2 1 Calibration constants developed for the instruments will be imported into the database This will produce a history of what has been done and when it was done Secondly it will be use to check for unusual trends in calibration for instrumen
36. ts on a calibration to calibration cycle Ocean Research amp Conservation Association 7 of 21 1 3 Chemical Reagents 1 3 1 A system of tracking each received reagent is employed to ensure proper tracking of chemicals used throughout the system 1 4 Calibration Timelines 1 4 1 The ORCA database holds the calibration timestamps and constant to identify timeframes when an instrument must be returned to the laboratory for maintenance calibration 1 5 Maintenance Instrument Reports 1 5 1 Each field maintenance trip report will be recorded in the database to create an easily accessible record of operations involving specific moorings and instruments 2 0 Kilroy 2 1 Board Inspection 2 1 1 The mother board and sensors are striped from the housing which can then be cleaned and refurbished A testing communications cable is connected to the board and a PC and communications are verified The pressure and temperature sensors are reconnected to the board and the device is polled for the Analog to Digital Converter values coming from the sensors these values are compared to expected values and recorded to verify that the sensors are operational before moving onto more thorough testing 2 2 Housing Inspection 2 2 1 The aluminum housing is cleaned of any bio fouling and inspected for any signs of corrosion Zinc anodes are removed and either discarded or set aside for reuse depending on condition Acoustic legs are replaced along with pressure and temperature se
37. ty FT 1500 DO and FT 1900 field continuous monitoring prior to the site visit and then verified after the monthly site visits Verifications for pH and salinity will be conducted with standards that bracket the sample readings from the field If post sampling verifications do not meet acceptance criteria listed associated sample data will be qualified with J estimated Any deployed instrument found out of calibration will be returned to the lab for calibration and check before redeployment Calibration checks will be recorded in the ORCA database prior to and after recalibration At each monthly site visit grab samples will be obtained by ORCA team members Billy Wells and Morgan Marmitt Grab samples will be sent overnight to PACE Analytical Services Inc for comparability assessment of Nitrate Nitrite Orthophosphate and Chlorophyll a At each monthly maintenance check reagents for the NuLab nutrient monitoring device will be replenished and a grab sample will be obtained for verification 6 2 Measurement Checks Data from deployed Kilroy Monitoring systems will be reviewed on a daily basis by ORCA engineers for data accuracy In addition the database will monitor for missing devices and moorings and generate automated alerts via email as necessary Data will also be reviewed to recognize any trends or drift of the instrument data Ocean Research amp Conservation Association 21 of 21
38. used standard When quinine sulfate standards are required in the future perform another dilution of the concentrated solution 3 11 9 Effect of temperature on fluorescence 3 11 10 The intensity of the fluorescence of many dyes shows an inverse relationship with temperature This effect must be accounted for when calibrating the EXO fDOM sensor with Quinine Sulfate Solution Enter the QSU or RFU value from the table below that corresponds to the temperature of the standard 3 11 11 Do not leave sensors in quinine sulfate solution for a long time A chemical reaction occurs with the copper on the sonde wiper assembly sonde bulkhead copper tape that degrades the solution and causes it to drift Also start with very clean sensors as the Ocean Research amp Conservation Association 15 of 21 presence of chloride and halide ions from estuarine or seawater conductivity standards and Zobell solution can compromise QS fluorescence 3 11 12 QSU 1 or 2 point 3 11 13 Pour the correct amount of clear deionized or distilled water into the calibration cup Immerse the probe end of the sonde in the water 3 11 14 Calibrate gt fDOM gt QSU ppb gt 1 or 2 point calibration 3 11 15 Enter 0 for first standard value and 300 ug L for second standard value Click Start Calibration Observe the readings under Current and Pending data points and when they are Stable or data shows no significant change for approximately 40 s
39. ve EOL end of life dates that are beyond date of first maintenance cycle 6 Register reagents being used in database before deployment via engineering web interface 7 Allow at least 1 hour for reagents to equilibrate before testing 8 DO NOT leave the system running without physically monitoring the first measurement cycle in case of a leak or pinched line Ocean Research amp Conservation Association 18 of 21 Procedure 1 Initial Reagent Blank As with any reagent change a reagent blank needs to be re calculated to compensate for any nutrients potentially present within the system The inlet line must be placed in D I de ionized water for a proper blank to be obtained A standard 70 micron filter needs to be installed as well in the event a contaminant gets into the sample as well as loading the mechanics with equivalent resistance to be seen in the field Once configured run a 5 measurement reagent blank Run both Prime and Inlet Flush macros to ensure no air is trapped in the lines Enter values in the table below after completion the dd KION command will display the results Ifthe OBS is out of range then re activate the cadmium column and repeat the test Initials Date Sample OBS 0 03 0 04 Absorption lt 0 008 1 2 3 4 5 Average Abs Deviation D I Creation Date Note 2 DO NOT wipe the records on the KION resulting from the testing as these w
40. w A standardized checklist will be provided as a reminder to the field crews of necessary operations Record keeping will be handled via a web interface into the database This will allow us to correlate mooring and device operations with the measurements being taken Hand sampling will be entered as well as a secondary measurement reference 5 2 Post Mooring visit 5 2 1 Field maintenance notes will be transferred to the database shortly after the visit to the mooring These notes will be correlated with the call button times to maintain temporal coordination Both mooring and device specific notes will be kept and entered into the database 5 3 Device Specific operations 5 3 1 Kilroy The Kilroy needs to be visually inspected every 4 weeks Inspection Replacement of anti fouling mechanism for depth sensor Re application of anti fouling compound on the acoustic sensor pods as needed Every 6 months the instrument will be returned to ORCAs manufacturing facility for recalibration of depth temperature and acoustics 5 3 2 YSI EXO2 Multi parameter Sonde Visual inspection every 4 weeks Laboratory calibration every 6 months Return YSI for recalibration should the laboratory calibration indicate further attention warranted 5 3 3 Green Eyes NuLAB Chemical reagent change every 4 6 weeks Each change will result in a reagent blank being created as well as an on board standard being updated Installation of new sample chamber filters as n
41. y screen and QC score 3 10 40 Click Exit to return to the sensor calibration menu and then the back arrows to return to main Calibrate menu Ocean Research amp Conservation Association 14 of 21 3 10 41 Rinse the sonde in tap or purified water and dry the sonde 3 11 EXO2 fluorescent dissolved organic matter DOM Sensor 3 11 1 Before calibrating be certain that the sensing window is clean cleaning instructions section 6 10 of EXO User Manual This procedure calibrates fDOM Relative Fluorescence Units RFU or DOM Quinine Sulfate Units QSU ppb If the user has both units selected then this procedure must be performed twice once for each unit to completely calibrate the parameter 3 11 2 For 2 point calibrations the first standard must be clear water 0 ug L The second standard should be a 300 ug L quinine sulfate solution Detailed mixing instructions 3 11 3 This reagent is particularly dangerous Remember that only trained personnel should handle chemicals 3 11 4 Preparation Use the following procedure to prepare a 300 ug L solution of quinine sulfate 300 QSU that can be used to calibrate the EXO fDOM sensor for field use 3 11 5 Purchase solid quinine sulfate dihydrate with a high purity gt 99 Recommended supplier Fisher Scientific item 6119 70 6 Purchase 0 1 N 0 05 M sulfuric acid to avoid the hazards of diluting concentrated sulfuric acid to make this reagent Recommended supplier Fisher Scientif
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