OXYGEN MICROOPTODE USER MANUAL
Contents
1. 7 UNISENSE OXYGEN MICROOPTODE USER MANUAL Oxygen MICROOPTODE USER MANUAL OXYGEN MICROOPTODE USER MANUAL Copyright 2013 Unisense A S Version August 2013 UNISENSE A S TABLE OF CONTENTS SuPPORT ORDERING AND CONTACT INFORMATION OVERVIEW u se atk a utasa Sy a E le are ee GET TING STARTED u ur aaa ga ss UNPACKING A NEW MICROOPTODE CONNECTING THE MICROOPTODE MEASUREMENT 5 aaa HANDLING OF MICROOPTODES INTERFERENCE STORAGE AND STORAGE CLEANING THE SENSOR APPENDIX EQUILIBRIUM O CONCENTRATIONS WARRANTY AND LIABILITY NOTICE TO PURCHASER This product is for research use only Not for use in human diagnostic or therapeutic procedures WARNING Microsensors have very pointed tips and must be handled with care to avoid personal injury and only by trained personnel Unisense A S recommends users to attend instruction courses to ensure proper use of the products WARRANTY AND LIABILITY The oxygen MicroOptode Sensor is covered by a 1 year limited warranty or 1 million data points estimated 300 hours of measurements Wear off of dye resulting in reduced signal guality e g from profiling into biological matrices like sediments and bend and breakage of the fiber optic cable is not covered by the warranty MicroOptodes are consumables Unisense will only replace dysfunctional sensors if they have been teste2. d in accordance with the instructions in the manual within 14 days of receipt of the sensor s The warranty does not include repair or replacement necessitated by accident neglect misuse unauthorized repair or modification of the product In no event will Unisense A S be liable for any direct indirect consequential or incidental damages including lost profits or for any claim by any third party arising out of the use the results of use or the inability to use this product Unisense mechanical and electronic laboratory instruments must only be used under normal laboratory conditions and a dry and clean environment Unisense assumes no liability for damages on laboratory instruments due to unintended field use or exposure to dust humidity or corrosive environments REPAIR OR ADJUSTMENT Sensors and electrodes cannot be repaired Equipment that is not covered by the warranty will if possible be repaired by Unisense A S with appropriate charges paid by the customer In case of return of equipment please contact us for return authorization For further information please see the document General Terms of Sale and Delivery of Unisense A S as well as the manuals for the respective products 6 CONGRATULATIONS WITH YOUR NEW PRODUCT SUPPORT ORDERING AND CONTACT INFORMATION The Unisense MicroOptode is an oxygen measuring system based on the latest development within optical fiber technology Use the MicroOptode for fast and accurat
3. d setup using the Unisense lab stand LS18 and micromanipulator MM33 MM33 2 to avoid damages on the MicroOptode When the optical fiber is retracted into the needle in protected position the MicroOptode can be pushed through a relatively hard surface such as a rubber septum The optical fiber can then subseguently be adjusted to the exposed position when the needle tip is positioned in the environment Please avoid bending of the fiber cable 13 INTERFERENCE The oxygen sensitive dye on the optic fiber shows excellent brightness and their red light excitation significantly reduces stress in biological systems and interferences caused by autofluores cence The MicroOptodes can be applied in gas phases agueous solutions ethanol methanol and isopropanol Other organic solvents and gaseous chlorine CI induce interferences with the sensor reading No cross sensitivity is found for pH 1 14 CO CH H S and any ionic species STORAGE AND MAINTENANCE STORAGE Store the sensor in the protective plastic tube used for shipping and store it in a dry dark and secure place CLEANING THE SENSOR All oxygen MicroOptodes can be cleaned with peroxide 3 H O soap solution or ethanol and can furthermore be sterilized with ethylene oxide 15 TROUBLE SHOOTING Problem Possible cause 1 Solution Problem Possible cause Solution Problem Possible cause Solution Drifting signal Can indicate photobleachin
4. e oxygen measurement in a broad variety of research applications If you wish to order additional products or if you encounter any problems and need scientific technical assistance please do not hesitate to contact our sales and support team We will respond to your inquiry within one working day E mail sales unisense com Unisense A S Tueager 1 DK 8200 Aarhus N Denmark Tel 45 8944 9500 Fax 45 8944 9549 Further documentation and support is available at our website www unisense com REPLACEMENT OF MICROOPTODES Unisense will replace MicroOptodes that have been damaged during shipment provided that The MicroOptodes were tested immediately upon receipt in accordance with the delivery note and the manual The white seal is still intact The MicroOptodes are returned to Unisense for inspection within two weeks The MicroOptodes are correctly packed for return to Unisense in accordance with the note included in the MicroOptode shipping box RECOMMENDED METERS Unisense MicroOptode Meter OVERVIEW WARNING Unisense MicroOptodes are This manual covers all the Unisense MicroOptodes The neither intended MicroOptode relies on the latest developments within optical fiber nor approved for technology utilizing near infrared dyes for improved performance use on humans high precision high reliability low cross interference and fast response times The MicroOptodes are designed for research applications with
5. g of the dye Recalibration of the MicroOptode High signal Can occur from too much ambient light exposed to the MicroOptode Darken the surroundings Low signal Broken tip Replace the MicroOptode If you encounter other problems and need scientific technical assistance please contact sales unisense com for online support we will answer you within one workday APPENDIX EQUILIBRIUM O CONCENTRATIONS Detailed tables are available at our web page http www unisense com support tables html At 20 C and 1 atm 1 umol O l 0 032 mg O I 0 024 ml O Table 1 Equilibrium concentrations of oxygen umol O litre at ambient partial pressure of 0 21 atm in water as a function of temperature and salinity Sources Garcia and Gordon L I 1992 Limnol Oceanogr 37 1307 1312 Millero F J and Poisson A 1981 Deep Sea Res 28A 625 629 17 19 UNISENSE UNISENSE DENMARK www unisense com info unisense com
6. ignal reading is the calibration value for zero oxygen partial pressure S The value should be less than 10 of the signal for atmospheric saturation otherwise see Troubleshooting When the calibration procedure has been successfully complete test that the MicroOptode responses as expected by inserting it in oxygen saturated solution and anoxic solution If the MicroOptode meets these reguirements the white seal can be removed CALIBRATION As oxygen MicroOptodes respond linearly to changes in oxygen concentrations a two point calibration is sufficient MEASUREMENTS HANDLING OF MicRoOPTopEs NOTE The MicroOptode fiber can be either retracted into the needle or Avoid bending of exposed the flexible design of the MicroOptodes enables optimal the cable as this positioning of the optic fiber tip in various samples might break the MicroOptode Locking a E d optical fiber n xpose optical Needle Optical fiber cable Adjustment ofthe fiber position ADJUSTING THE EXPOSURE OF THE OPTIC FIBER Positioning of the optical fiber is adjusted by loosening the locking nut on the MicroOptode and pushing the optical fiber cable towards the plastic shaft The optical fiber can be pushed maximally 1 cm out from the needle protection Tighten the locking nut after positioning the optical fiber MouNTING OF THE MICROOPTODE When the optical fiber is exposed Unisense recommends mounting the MicroOptode in a stabilize
7. in physiology biotechnology environmental sciences and related areas MEASURING PRINCIPLE The very tip of the optical fiber is coated with a specialized dye that is excitable with red light 610 630 nm When the MicroOptode is connected to a Unisense MicroOptode Meter the dye is excited with sinusoidal modulated red excitation light The dye show an oxygen dependent luminescence in the near infrared NIR 760 790 nm and by detecting guenching of the luminescence the oxygen concentration can be determined in the sample Measuring principle The near infrared dye shows oxygen dependent gt luminescence in the near infrared NIR after RedHight exposure to excitation light High NIR emission at low oxygen l concentration and B low at high oxygen concentration GETTING STARTED WARNING Do not remove the seal and protective UNPACKING A NEW MICROOPTODE plastic tube N 2 N N before these steps The MicroOptode sensor is shipped in a protective tube with the calibration optic fiber positioned half exposed in the needle Please do not successfully remove the white seal or move the fiber further out of the needle completed before the calibration procedure has been successfully completed CONNECTING THE MICROOPTODE Remove the black cap from the MicroOptode connector and remove the red cap from the MicroOptode Meter Insert the male fiber plug of the MicroO
8. ptode cable into the ST receptable of the MicroOptode Meter and turn the bayonet coupling gently clockwise until the plug is locked firmly The MicroOptode and the connecting male fiber plug CALIBRATION Please consult the software manual for instructions on how to calibrate the MicroOptode in the software Calibration of the oxygen MicroOptode sensor is performed as a two point calibration by measuring the MicroOptode response in a solution saturated with oxygen and an anoxic solution Remember that a temperature sensor is required for correct oxygen measurements unless the measurement and calibration is completed at stable temperature OXYGEN SATURATED SOLUTION Place the MicroOptode in a well aerated calibration solution e g by bubbling with air in the Unisense calibration chamber After 5 min of vigorous bubbling turn off the air follow the MicroOptode signal and when the signal is stable add the point to the calibration in the software This signal is your calibration value for 100 oxygen saturation at atmospheric partial pressure Zero reading ZERO READING An anoxic solution can be prepared in one of several ways below we describe two methods recommended by Unisense 1 Prepare a solution of sodium ascorbate and NaOH both to final concentrations of 0 1M 2 g sodium ascorbate in 100 ml of 0 1M NaOH This zero calibration solution can be stored in a closed container for 1 2 weeks Keep the MicroOptode in its pro
9. tective tube and expose only the fiber tip and the needle part of the sensor to the ascorbic solution place the MicroOptode in the anoxic solution read the signal and add the point to the software calibration Clean the MicroOptode thoroughly in either tap water or demineralized water to avoid any carry over of ascorbic solution to your sample 2 Vigorous bubbling with oxygen free inert gas e g N It is important to apply vigorous bubbling over a time period gt 5 min sufficient to ensure that all oxygen has been removed Furthermore it is important to prevent any contact of oxygen with the water during bubbling as oxygen will otherwise be continuously reintroduced to the water In practice this means that the headspace above the water must be closed except for a hole slightly larger than the MicroOptode shaft This effectively prevents IMPORTANT Calibration must be performed after pre polarization when the sensor signal has stabilized Always use acalibration solution with the same temperature and salinity as the sample solution IMPORTANT The O sensor signal is sensitive to temperature and the O solubility depends on both salinity and temperature 11 ambient air from entering the vessel We recommend the Unisense calibration chamber CAL300 Place the MicroOptode in the anoxic solution read the signal and add the point to the software calibration Place the MicroOptode in the anoxic solution The s
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