user instructions - Dolomite Microfluidics
Contents
1. MITOS P PUMP FLOW CONTROL imi USER INSTRUCTIONS The Dolomite Centre Ltd 1 Introduction 3 2 Fluidic Connections 4 3 Electrical Connections 5 4 Manual User Control 7 5 PC Software User Control 11 6 General Notes on Use 12 Appendix A Updating the Software 13 MAR 000051 v1 3 Page 2 of 14 EDD B 1 Introduction These user instructions describe how to use the Mitos P Pump with flow sensor connected in line in flow control mode Flow control mode allows a flow rate to be selected instead of a pressure and the P Pump pressure is automatically adjusted to achieve the target flow rate The benefit of flow control mode is that it accounts for changes in the flow resistance of the system to maintain constant flow rate To operate in flow control mode the Mitos Flow Rate Sensor Part No 3200096 3200100 must be connected to the Mitos P Pump using either the Mitos Sensor Display Part No 3200095 or Mitos Sensor Interface Part No 3200200 The table below shows the compatible configurations for newly purchased hardware Mitos Mitos P Pump Mitos P Pump Mitos P Pump Sensor Interface Type P Pump Basic Remote Remote Basic 3200016 3200175 3200176 3200177 Mitos Sensor Display Part No 3200095 Mitos Sensor Interface Part No 3200200 Notes For users who already own Dolomite products the following hardware compatibility applies e P Pumps with serial numbers lower than 160290 are not compatibl2. 0 a H a T Tare and leak no tips Misc test functions avallable in OS IDLE mode Select fluid type Stop flow and go to when in IDLE mode IDLE mode MAR 000051 v1 3 Page 11 of 14 The Dolomite Centre Ltd 6 General Notes on Use 6 1 System Stability The first rule for setting up a system is that it should be stable in pressure control mode for it to be stable in flow control mode Therefore the correct flow resistors should be selected to provide the desired flow rate range and stability for 2 phase systems with fluids of different viscosities An unstable system means that the flow rate is oscillating around the target value but is unable to reach stability The indicator is shown on the left Why is my system unstable e The flow rate selected requires a very low P Pump pressure e g lt 20 mb in order to reach the target The solution is to insert additional flow resistance into the system to increase the pressure required to reach the flow rate target e The flow rate selected requires a higher P Pump pressure than supply pressure in order to reach the target In this case the current flow rate displayed will always be lower than target The solution is to reduce the flow resistance in the system to reduce the pressure required to reach the flow rate target e he system is inherently unstable It is always possible to set up unstable systems when using 2 or more fluids of different viscosities o
3. no software update is required P Pumps and Sensor Units purchased before 1 July 2012 will need to be updated to the latest version of software Only P Pumps with a serial number greater than 160290 can be upgraded Please contact us for any enquiry regarding updating the software Updating P Pump Basic The P Pump Basic can be updated using the update icon in the PC software see section 6 and browsing to the update file provided by Dolomite MAR 000051 v1 3 Page 13 of 14
4. changing control mode This feature allows the user to set up a system using pressure control and then switch to flow control Or vice versa 4 4 Fluid Calibrations The Mitos Flow Rate Sensors are calibrated to provide an accurate reading when used with water The fluid menu option allows an alternative fluid type to be selected from a list of 4 common fluids Selecting an alternative fluid ensures that the flow sensor reading is now accurate for the selected fluid This menu option only appears when a Mitos Flow Rate Sensor is electrically connected to the Mitos P Pump see section 3 In the main menu select option 2 to select a calibrated fluid type Phe 4 Scroll to select the fluid type A star appears next to the current fluid type Mae Mese Fue a NU 200 0 2 m FC40 FC 40 Fluorinated liquid as used in Picosurf products NOVE Novec 7500 Hydrofluoroether as used in Picosurf products It should be noted that if the flow sensor type is subsequently changed then the fluid calibration will revert back to water and it will be necessary to re select if an alternative fluid is required MAR 000051 v1 3 Page 9 of 14 The Dolomite Centre Ltd 4 5 Taring Flow Rate Sensor It is recommended to tare the flow rate sensor after the system is set up and connected If a flow rate sensor tare is performed with little fluidic resistance connected then the tare may be inaccurate disconnect pneumatic In the main menu
5. e For example the Mitos Flow Rate Sensor 1 50 ul min Part No 3200098 has flow rate range of 0 2 50 ul min for the user to select current flow rate aM E 3E al 2 select flow rate click scroll click to select Unstable indicator Coefficient of Variation gt 596 E Js Initially the flow rate E will be unstable 5 E 5 Stable indicator Coefficient of Variation 596 After a short period the flow rate becomes stable EIL L8 scrolls to show current pressure wait 30 seconds Screensaver mode Scrolls between calibrated fluid target flow rate and current flow rate and pressure The stability indicator appears next to the flow rate value showing that the pump is in flow control mode When in pressure control mode the stability indicator appears next to the pressure value MAR 000051 v1 3 Page 8 of 14 The Dolomite Centre Ltd 4 3 Default Target Values When Changing Control Mode The user can change between pressure and flow control modes without stopping the flow When changing from flow control mode to pressure control mode the default target pressure will be the current pressure when the change is made 60 mb in the example above Similarly when changing from pressure control mode to flow control mode the default target flow rate will be the current flow rate when the change is made In this way there is minimal disruption to the flow system when
6. e with any flow sensor interface e P Pumps with serial numbers greater than 160290 but lower than 160444 are only compatible with Mitos Sensor display e P Pumps with serial numbers greater than 160444 are compatible with any flow sensor interface MAR 000051 v1 3 Page 3 of 14 EDD C 2 Fluidic Connections The flow rate sensor should be connected between the Mitos P Pump and the microfluidic system as shown below Connect flow sensor to system Connect P Pump to flow sensor Mitos Flow Rate Sensor connected to a Mitos P Pump When using the Mitos P Pump in flow control mode flow resistors will be required Flow resistors allow the user to bring a system into the desired flow rate range In experiments such as droplet generation where 2 fluids of different viscosity meet at a junction flow resistors are needed to provide stability Mitos Mitos Flow Flow P Pump Rate Sensor Resistor Mitos Mitos Flow Flow P Pump Rate Sensor Resistor Use of flow resistors in a flow control system diagram Microfluidic Chip MAR 000051 v1 3 Page 4 of 14 M Qs 3 Electrical Connections To enable flow control a Mitos Flow Rate Sensor must first be connected to either a Mitos Sensor Display or Mitos Sensor Interface Then connection must be made to the Mitos P Pump Electrical Connection of Mitos Flow Rate Sensor to Mitos Sensor Display or Mitos Sensor Interface Slide Mitos Flow Rate Sensor into position ensur
7. ing that the latch has clicked closed Electrical Connection of Mitos Sensor Display to the Mitos P Pump Use USB cable supplied with the Mitos Sensor Display Please note that only the top USB port of the Mitos P Pump can be used for connection of the Mitos Sensor Display MAR 000051 v1 3 Page 5 of 14 EDD B o a Electrical Connection of Mitos Sensor Interface to the Mitos P Pump Use circular multi pin cable attached to the Mitos Sensor Display to make connection to the Mitos P Pump MAR 000051 v1 3 Page 6 of 14 The Dolomite Centre Ltd 4 Manual User Control 4 1 Changing to Flow Control Mode The ctrl control menu option allows the user to switch to flow control mode This menu option only appears when a Mitos Flow Rate Sensor is electrically connected to the Mitos P Pump see section 3 Main control screen P indicates pump is in pressure control mode click to access menu In the main menu select option 1 to change to flow control In the control menu select option 1 to change to flow control Select Y to confirm F indicates pump is in flow control mode Press the back button to return to the main control screen MAR 000051 v1 3 Page 7 of 14 EDU UO 4 2 Setting Target Flow Rate When in flow control mode a flow rate can be selected based on the flow rate sensor type connected The available flow rate range is 20 of the minimum value to the maximum valu
8. r high flow rate ratios In general for 2 phase systems the flow resistance before the junction should be significantly greater than the flow resistance after the junction 6 2 Correction Factors for Alternative Fluids The Mitos Flow Rate Sensors are calibrated for water and 4 common fluids In order to obtain accurate flow rates for alternative fluids it is necessary to use correction factors to convert the displayed value into the actual value A method of providing a known flow rate is required to work out the correction factor for the selected fluid This could be a syringe pump or a P Pump delivering fluid onto a precision balance with volume calculated from known density For each flow sensor type it is necessary to pump the selected fluid through the flow sensor at a minimum of 5 different flow rates in the range and record the displayed value For example with the 1 50 ul min sensor displayed readings could be recorded at flow rates of 5 15 25 35 and 45 ul min This data can be plotted as a curve of actual flow rate against displayed flow rate Alternatively the correction factor can be calculated by dividing actual flow rate by displayed flow rate The correction factor can then be plotted against displayed flow rate and used to adjust the flow rate values for this selected fluid MAR 000051 v1 3 Page 12 of 14 EDD C Appendix A Updating the Software Flow control is ready to use on all new P Pumps and Sensor Units and
9. select option 3 to perform a tare Select option 2 to tare the flow sensor only or option 3 to tare the flow and pressure Click to confirm that supply is disconnected Click to start tare MAR 000051 v1 3 Page 10 of 14 EDD C 5 PC Software User Control The Mitos Flow Control Centre software enables flow control to be performed from a PC Mitos P Pump and Mitos P Pump Remote pumps connect to your PC via a standard USB cable Mitos P Pump Basic and Mitos P Pump Remote Basic connect to your PC via a serial RS232 cable or USB to RS232 adapter cable More information on detecting hardware is available in the Mitos P Pump Range User Manual o i n I FL ow A me Lj Ld udi i ONI ROL bied Stop flow a Pump State A i Label name can E _ E ioni er be edited to B 8 FLOW s eui eal identify pump CONTROL control mode CO Pressure Control Moni l onitor current Click on icon to pe 2 E Target mbar n pressures update software Type P Pump Chamber 132 mbar when in flow for P Pump Version 1 0 54 V sup 883 mbar control mode basics only Serial Number 12345 CZ Flow Control EN S uc me Set target and sor arget p min monitor current Details of current Type 3200087 Current 497 pl min flow rate when in connected flow Range 30 1000 l min Be flow control mode sensor type Fluid Calibration Vater
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