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MYECL™ Imager Manual - Thermo Fisher Scientific

1. 18 MYECL Imager User Manual Thermo Fisher Scientific 19 Set Image Gallery Location Allow Remote Access MYECL Imager User Manual The Set Image Gallery Location button allows the administrator to set up a default pathway for saving acquired images The imager has a default image gallery location Computer Local Disk D Gallery_1 The image gallery location can be changed to another folder in a local or network drive When Set Image Gallery Location is selected the program automatically creates a Gallery_1 folder if there are no previously saved images in the selected folder 1 Select the Set Image Gallery Location button 2 Browse for the appropriate gallery location using the folder menu 3 Select OK after choosing the appropriate storage location Browse For Folder Please choose Gallery Folder to save captured images Desktop EN E My Documents E3 My Computer D My Network Places o Recycle Bin iC myECL j Cancel Remote access for maintenance and troubleshooting is possible with the imager After selecting the Allow Remote Access button the administrator views the following screen x You have selected to allow remote access by Thermo Scientific Pierce Technical Support Please call one of the following numbers to speak to a Technical Support Scientist and initiate a remote access session USA 800 8743723 or 1 815 9680747 Europe 49 228 8502820 Asia Pacific 81 3 58
2. Quick start guide USB memory device otylus otylus holder Power cord with C13 connector Optional items including additional filters and UV bulbs 365nm may be added to the above list depending on the items ordered with the imager Note Upon receiving your imager please verify you have received all of the items listed above Please contact Thermo Scientific Technical Support for assistance Thermo Fisher Scientific Functionality The MYECL Imager provides UV and white light illumination source for imaging of samples Acquisition of chemiluminescent Western blot and electrophoresis gel images with varying resolution 1x1 2x2 3x3 4x4 and 8x8 binning Low noise high dynamic range CCD camera detection Hardware and software optimized for image acquisition One touch image acquisition Multiple image acquisition with pre set and user defined settings Thermo Scientific Each imager arrives loaded with the myECL Imager Acquisition Software This MYECL Imager manual gives detailed information on using all software functions and modes Acquisition Software The acquisition software allows you to acquire images using three different modes Chemiluminescence Chemi mode for image acquisition of self emitting chemiluminescent light Ultraviolet UV mode for image acquisition of UV transmitted light Visible Vis mode for image acquisition of reflected and or transmitted light 3 MYECL Imager User Manual Thermo Fisher Sc
3. TM f Thermo SCIENTIFIC Table of Contents Chapter 1 Overview 1 Thank You and Welcome Letter eee 1 Contacting Thermo Fisher Schenttte ee 2 Imager Overview ou sss 2 Included Accessories eese 2 PU EEn TA A E A E AE A E AE EA 3 MYECL Imager Acquisition Software eene 3 mylmageAnalysis Software sss 4 Chapter 2 Imager cscs 5 Installation Guidelines 5 SEET eeneg 5 Electrical e HIE eee 5 Environmental Requirements 6 Computer Requirements eene nennen nnne 6 Imager Images with Feature Locations nne f ina Uc A E 10 OT et f 11 USB Features sss 12 Imager Gettnge 0 cescessesseesseesseesesiesisessesees 13 ln 13 Date and Hr 14 Nol 15 SU 16 Administrator Settings esse 18 Set Image Gallery Location eeeemmemmem 19 Allow Remote ACCESS adici ba oen BACON IR Om UR SM UE aeea 19 Other Operating System OS Settings esee 21 Reset Default Focus Position enne 21 Update Software ebe 21 Change Username and Password eee 21 Create Dark and Bias Images eem 22 BOUT oes iem m E E 24 Chapter 3 Image Acquisition 25 Imager Settings 25 LVO G aO E 25 Lus Camera in Chemi EE
4. United States Tel 815 968 0747 or 800 874 3723 Customer Assistance E mail Pierce CS thermofisher com thermoscientific com pierce Therm SCIENTIFIC Part of Thermo Fisher Scientific
5. 250V 4A Table 2 3 Imager electrical requirements for major countries Region Voltage Frequency Amps A VAC Hz US and Canada 120 60 1 0 Europe 220 50 0 5 UK 240 50 0 5 Japan 100 50 1 2 China 220 50 0 5 India 230 50 0 5 South Korea 220 60 0 5 Environmental For optimal performance maintain a constant laboratory temperature range of 15 Requirements 29 C 59 77 F with relative humidity between 15 70 To allow adequate camera cooling do not place the imager in direct sunlight Computer Requirements The imager is operated by an integrated computer which is accessed through the touchscreen The mylmageAnalysis Software program is included with the imager purchase To run the mylmageAnalysis Software properly workstations must meet the minimum performance requirements listed in Table 2 4 Table 2 4 Recommended computer requirements for MYImageAnalysis Software Description Recommended Operating System Windows 7 Vista or XP Processor Pentium 4 2 4GHz Memory RAM 512MB Hard Drive 2GB screen Resolution 1280 x 1024 Refer to the MYImageAnalysis Software User Manual for more information 6 wvECL Imager User Manual Thermo Fisher Scientific Imager Images with Feature Locations Touchscreen Imager Front View Upper USB Port Soft Power Switch SCIENTIFIC ll Lower USB Ports Sample Chamber Door j MYECL Imager Figure 2 1 Imager front and side views detailing main components 7 MYECL Imager
6. Image Info MYECL Imager User Manual Information for each captured image is stored within the individual xml file With the captured image displayed select the Image Info button A box appears with all of the relevant image information for that image Beneath the box is a keyboard for inputting personal data into the open fields Only the fields for File name User Project and Notes can be modified Note After each image capture the imager will assign a default name to the image file This image file name can be changed within the Acquisition Information screen Acquisition Information File name UV 200ms 20121004 0351 User mE Project RN Exposure time 200ms Mode UV Acquisition time 3 51 AM Acquisition date 2012 10 04 Camera temperature 250C 1x1 binning Sensitivity resolution File name Image title imager generated or user defined User Person using the MYECL Imager user defined Project Name of project user defined Notes helevant notes pertaining to image or experiment user defined Exposure time Chosen exposure time for image Thermo Fisher Scientific 45 MYECL Imager User Manual Export Print Delete Mode Single multiple custom UV or Vis Acquisition time Time of day image was taken Acquisition date Date image was taken Sensitivity Resolution Binning used for image acquisition select Export to save the highlighted image to a USB flash drive External flash drives
7. Select the point of interest on the image to display the estimated pixel intensity of the corresponding pixel coordinates The displayed pixel intensity estimated is updated when the exposure time is further adjusted Interactive Chemi r 09m 4ts Long Short Exposure Calculated Display Exposurt Saturation 6 Select the Acquire button to capture an image with the indicated exposure time Select the Cancel button to return to Chemi mode Thermo Fisher Scientific Capture Settings Single Image Acquisition ig N Select the Chemi tab on the touchscreen Optional Select the Live Camera button if the live image is not displayed The epi white lights and live image display are activated Open the imager door pull out the transilluminator and install the chemiluminescence exposure screen black on the transilluminator glass Insert and center the sample on top of the chemiluminescence exposure screen black using the live image display for reference Close the imager door While viewing the live image adjust the settings for Focus Digital Zoom and Resolution Sensitivity in the Camera Settings if needed Note Camera Settings are available only with the live image display select a Pre set or Custom exposure time Note ChemiV images are captured and automatically saved to the Gallery however they are not displayed in the image tab if Live Camera is not enabled before image acquisiti
8. Reflected Light usa ooa ya o rh ern ro rr hr rn a 59 Pre set EXDOSHIOTIIO cts cceeeieceeceteeeenateeeseaeteak sen einene LE EP LE 59 EIST E 54 VAG te 54 Display Saturation ccccccceeeeccceeeececeeeeeeceeeeeeeeeeeseneeeeeenaeeeeeaeeeeneaeeeeeeas 55 JET E 59 e E 56 D EE TEE 56 Bien Thy tege 56 E 56 za E 96 image Tu RENTRER 56 Su RR 56 XOT RR 57 Image Gallery 57 Acquisition Information 57 ie VIC WING sees 58 To ET 58 leegent 59 OO oee acinaiesecinsttee spaaaitrastsestad AS DUNS OH tU MEER AC DUE LM EIN LEIEI 59 Segre E IC DU FE NANAON E 60 TURNER 60 WINNER 60 SUERTE RR 61 SEITE RTT 61 U a 61 B t File Manager EQ E 61 Chapter 4 Maintenance 62 Cleaning the Imager and Sample Screens 62 LCD Touchscreen sss 62 Use of the Stylus 62 UV Transluminator cc ea 63 Band EXCISION EE 63 Cleaning the Translluminator 64 Replacement of Iransilluminator Bulbs 64 Emission Filters ssssssssee 65 Changing PISIS iiis aseptpui t irtituti iuro taki Ere DbuaHuteiusn tr owe bue Das Ud Pda Che up Up 65 Appendix A Technical Specifications 67 Appendix B Arcceseortesg 20 ccccccccccccssccssssesssesseeeee 68 Appendix C Related Thermo Scientific Products c9 Glossary cc cc 70 1 Thank You and Welcome MYECL Imager User Manual Chapte
9. and vibration sources IMPORTANT The imager weighs 65lbs 29 5kg Use two people to lift it by grasping the bottom and the handle at the rear of the imager Using two people place the imager onto the benchtop given proper space electrical and environmental requirements Remove the outer and inner packaging materials The imager requires a stable laboratory bench or table capable of supporting 65lbs 29 5kg Provide a minimum clearance of 3 inches 62cm at the rear of the imager to allow adequate ventilation for camera cooling and access to the power cord and switch In addition allow sufficient space to open the front facing imager door to place sample within the imager Instrument dimensions are listed in Table 2 1 Table 2 1 Imager dimensions Dimensions Inches Centimeters Width 12 25 31 1 Depth 22 55 9 Height 21 53 3 A grounded circuit capable of delivering the appropriate current and voltage is required for installation Electrical requirements can be located on the imager label on the rear panel of the imager and in Table 2 2 The imager electrical system adjusts to the proper voltage for the respective country Table 2 3 Connect the imager power cord to the rear left side panel of the system and plug into a grounded power outlet Thermo Fisher Scientific Table 2 2 Imager electrical ratings Electrical Parameter Rating supply Voltage VAC 120 240 Frequency Hz 50 60 Power Rating W 120 Fuse power center T4AL
10. are the default settings for the imager Reset Button used to reset all imager settings to their default values 28 MYECL Imager User Manual Thermo Fisher Scientific Settings Only Available in UV and Vis Modes Camera Settings In Ol Out H Open Aperture Close i II 4 b m B I e oe Orange 565 620nm ve Reset Aperture Slider bar for adjusting the camera lens opening to control sensitivity and depth of field The aperture can be fully closed far right position or fully opened far left position depending on the light sensitivity needed Digital Zoom 4 Focus Qut UV Light Button for turning on off the transilluminator UV bulbs Filter Wheel Position Drop down menu for moving the filter wheel position to switch filters Note Filter Wheel Positions 2 4 can be set for different filter colors by accessing Tools gt Filters e Q Orange 545 630nm emmmer 1 Chemi 2 Orange 545 630nm SE 3 Green 515 560nm visp M 4 Red 620 645nm Saturatic 29 MYECL Imager User Manual Thermo Fisher Scientific Chemiluminescence Chemiluminescence Chemi Mode is the default mode Chemi mode is used for the capture of sample images using sample emitted light A visible image of the sample Chemi Mode i automatically captured in Chemi mode using reflected light ChemiV image which is useful for imaging colorimetric molecular weight markers Camera Settings Focus Digital Zoom and Resolution S
11. can access the imager through three different ports on the right side of the imager Note Users may need to momentarily wait for the imager to detect the external drive once it is connected to the USB port The selected image is exported only to the first USB flash drive if there are more than two drives connected to the imager The Print button allows the highlighted image to be printed to a selected external printer A Print Preview window appears before printing begins The Delete button permanently deletes the highlighted image Thermo Fisher Scientific Ultraviolet UV Mode 46 2 AA Camera Settings Capture Settings Image Acquisition MYECL Imager User Manual Ultraviolet UV mode is used for the capture of images using transmitted UV light CAUTION UV mode uses the UV light in the transilluminator Long term exposure to UV light can be harmful Use the appropriate eye and skin personal protective equipment PPE if there is any chance of exposure to the UV light Note The transilluminator UV light is initially on for 5 minutes User input is requested for further use of the UV light If the Keep UV on button is not selected the UV light automatically turns off lf the UV light is needed after opening the imager door the white door peg can be pulled out to turn on the UV bulbs Appropriate PPE must be worn if performing this operation The white door peg returns to a normal position once the door is fully close
12. conversion screen using the live image display for reference 5 Once the sample is centered close the imager door The UV light turns on and shows as visible light transmitting through the white light conversion screen 6 While viewing the live image adjust the necessary settings for Focus Digital Zoom Sensitivity Resolution Filter and Aperture to produce the desired image 7 Select a Pre set or Custom exposure time Thermo Fisher Scientific Image Acquisition Reflected Light Pre set Exposure Time 1 T J m select the Vis tab on the touchscreen Changing to Vis mode automatically turns on the UV lights within the transilluminator and the epi white lights Optional Select the Live Camera button and enable the UV button if the live image is not displayed Open the imager door confirm that the UV light is off and install the white light conversion screen on top of the transilluminator glass Insert and center the sample on top of the white light conversion screen using the live image display for reference Once the sample is centered close the imager door The UV light turns on select the UV light button on the right side of the screen to switch OFF the UV light This produces only reflected light While viewing the live image display adjust the Camera Settings for Focus Digital Zoom Resolution Sensitivity Filter and Aperture to produce the desired image if needed oelect a
13. filter for use in Chemi applications Position 2 Orange filter for use in UV and Vis applications Position 3 No filter Position 4 No filter The imager is supplied with an orange filter in Position 2 but green red and blue filters can also be purchased for additional applications Install additional filters into Positions 3 and 4 Assign filters to the different positions in the Filter Menu Selections in the Tools menu 1 Select the Filters button The following menu appears Thermo 2 chemi E uv 7 vis BS Gallery o e Serial Number KR01211106 Filter Menu Selections Position Description Wavelength NoFilter Orange 545 630n m Green 515 560nm Q Blue 445 480nm Save e Red 620 645nm 2 Positions 2 3 and 4 use selectable drop down menus to enable filter assignment Chemi empty Orange Green Blue and Red Select the choice for each filter position Thermo Fisher Scientific 3 When complete select the Save button at the bottom of the menu to save the changed defaults Filter Menu Selections Position Description Wavelength No Filter Chemi Chemi Orange 545 630nm Green 515 560nm Blue 445 480n m Save Red 620 645nm Table 2 5 Imager filter specifications and corresponding dyes Filter Color Wavelength Range Compatible Dyes sample Screen nm and Stains Blue 445 480 DAPI Clear screen AMCA Clear screen Green 515 560 SYBR Green Clea
14. is not displayed 3 he epi white lights and live image display are activated Open the imager door pull out the transilluminator and install the chemiluminescence exposure screen black on the transilluminator glass 4 nsert and center the sample on top of the chemiluminescence exposure screen black using the live image display for reference 5 Once the sample is centered close the imager door 6 While viewing the live image adjust the settings for Focus Digital Zoom and Resolution Sensitivity in the Camera Settings menu if needed Note Camera Settings are available only with the live image display 7 Select a Pre set or User defined Multiple Exposure Time Note A Stop button appears over the keypad once the Acquire button is selected Image acquisition can be stopped by selecting the Stop button 36 MYECL Imager User Manual Thermo Fisher Scientific 37 Pre set Multiple Exposure MYECL Imager User Manual Settings The five pre set multiple exposure settings allow you to select a time series of exposures Multi Exposure Settings Pre set Tap the button to capture images Exposure 1 Pre set 5s Pre set 10s Pre set RI Pre set4 im Pre set5 im 1 Select the Multi button from the touchscreen number keypad 2 10s 30s 5m 146m bm 10m 10m 15m 10m 4m 20m 30m X Total tm 16 40m 31 30m 38m im 2 Select one of the Pre set option buttons with an a
15. selection of Live Camera in Chemi mode turns on the epi white lights ChemiV images are captured and saved to the Gallery however they are not displayed in the image tab if Live Camera is not enabled before image acquisition in Chemi mode Thermo Fisher Scientific Live Camera in Selection of Live Camera in UV mode turns on the UV light The live image is UV mode displayed with the exposure time used in the previous image acquisition in UV mode unless the user changes the exposure time or changes the mode Live Camera in Selection of Live Camera in Vis mode turns on the UV and epi white lights The live Vis mode image is displayed with the exposure time used in the previous image acquisition in Vis mode unless the user changes the exposure time or changes the mode 26 MYECL Imager User Manual Thermo Fisher Scientific 2 Camera Settings Available in Chemi UV and Vis Modes MYECL Imager User Manual Thermo UV Vis Gallery SCIENTIFIC Chemi Live Display 2013 April 15 16 12 45 Camera Settings In cus ut OF I Out Digital Zoom In ah i E Note Camera Settings are only available with the live image display Focus Icon for performing minimal focusing adjustments that may be needed as varying object depths change Note An Imaging Reference Target is supplied with the imager Use this target in Vis mode when performing camera focusing Digital Zoom Icon for increasing the camera zoom level Slider bar
16. the exposure time using the pre set exposure time buttons 0 1s 0 2s 0 3s 0 4s 0 5s 0 6s 0 7s 0 8s 0 9s L De Custom Camera Settings Jw select the Acquire button to acquire an image Captured images are displayed on the screen and automatically stored in the Gallery for further viewing and selection Thermo Fisher Scientific Custom Exposure Time Select the Custom exposure time button using the touchscreen keypad 2 Enter the desired time in milliseconds ms seconds s or minutes m using the touchscreen keypad numbers The m s box is used to input seconds and or minutes and is active by default box is gray in color For a custom exposure time of 1 second select the ms box to input milliseconds The shortest exposure time that can be entered is 10 milliseconds selecting 1 from the Keypad appears as 10 in the ms box m s ms 1 2 3 4 5 I 6 7 8 9 Acquire T 0 Cancel Camera Settings Z w 3 Select the Acquire button to acquire an image 4 Captured images are displayed on the screen and automatically stored in the Gallery for further viewing and selection 48 MYECL Imager User Manual Thermo Fisher Scientific 49 Stop Image Acquisition Image Tools Display Saturation MYECL Imager User Manual Upon selecting the Acquire button a Stop window appears over the keypad selecting the Stop button stops the current image acquisition exposure and return the screen to th
17. the images 8 Captured images are displayed on the touchscreen and stored in the Gallery for further viewing and selection 39 MYECL Imager User Manual Thermo Fisher Scientific 40 Image Tools ChemiV Overlay Display Saturation MYECL Imager User Manual Note The Image Tools only function while viewing or printing the image ChemiV Overlay Display Saturation Invert Auto Contrast and Adjust Image adjustments are NOT saved with the image file After capturing an image in Chemi mode the ChemiV Overlay button can be selected to superimpose the corresponding ChemiV image on top of the Chemi image Image adjustment can be applied to the overlaid image and the intensity display from the original Chemi image can be displayed on the overlaid image The ChemiV Overlay button is enabled only when user is viewing a Chemi image Original Chemi Image With ChemiV Overlay In Chemi mode after capturing an image the Display Saturation button can be selected to highlight image areas that are considered to be saturated A red false color is superimposed on the areas deemed to be saturated Adjustment to the saturation can be done by adjusting the exposure time for subsequent images shorter exposure times reduce the light exposure saturation 1 After capture of an image select the Display Saturation button under the time Keypad 2 The screen displays the saturated pixels on the image with a red false color D
18. 26 1659 Close Thermo Fisher Scientific Follow the instructions provided and contact our Technical Support team for questions or to troubleshoot the imager U S contact 800 874 3723 International contact 1 815 968 0747 Europe contact 49 228 8502820 Asia Pacific contact 81 3 5826 1659 Note Remote access requires the imager to be connected to a network with internet access Contact your facility s IT department regarding correct set up and use of network connections 20 MyYECL Imager User Manual Thermo Fisher Scientific Other OS Settings This function allows the administrator to access the Windows Control Panel to change system and user configurations as needed Note Only qualified administrators should have access to this area Altering configurations can cause imager errors l n I O 2 OD 3 lt a Vis E Gallery ej Esc EE BEE K A L Control Panel All Control Panel Items gt Search Control Panel 2 CIENTIFI Adjust your computer s settings View by Smallicons v Action Center 1 Administrative Tools We Backup and Restore Credential Manager Date and Time e Default Programs ge Device Manager Devices and Printers P Display E Folder Options Ei HomeGroup e Indexing Options Internet Options Keyboard J Mouse E Network and Sharing Center Wi Performance Information and Tools E persona lization 3 Power Options A Programs and Features R
19. Connector United Kingdom Ireland T Replacement part for accessory supplied with the MYECL Imager Package Size 1 CD 1 filter 1 filter 1 filter 1 filter screen screen screen 1 bulb 1 bulb 1 package 1 package 1 cord 1 cord 1 cord Thermo Fisher Scientific Appendix C Related Thermo Scientific Products Product No 34087 34075 34095 46640 32209 32132 24615 88518 88018 88610 26616 26619 26614 26634 26625 26628 26612 69 wmyECL Imager User Manual Product Name Supersignal West Pico Chemiluminescent Substrate Supersignal West Dura Extended Duration Substrate Supersignal West Femto Maximum Sensitivity Substrate Supersignal Western Blot Enhancer Pierce ECL Western Blotting Substrate Pierce ECL Plus Western Blotting Substrate Imperial Protein Stain 1L PVDF Transfer Membrane 0 45um 26 5cm x 3 75m Nitrocellulose Membrane 0 45um 30cm x 3 5m Western Blotting Filter Paper extra thick 8 5cm x 9cm PageRuler Prestained Protein Ladder 10 170kDa 2 x 250uL PageRuler Plus Prestained Protein Ladder 10 250kDa 2 x 250uL PageRuler Unstained Protein Ladder 10 200kDa 2 x 250uL opectra M Multicolor Broad Range Protein Ladder 10 260kDa 2 x 250uL Spectra Multicolor High Range Protein Ladder 40 300kDa 2 x 250uL Spectra Multicolor Low Range Protein Ladder 1 7 40kDa 250uL Pierce Prestained Protein Molecular Weight Marker Thermo Fisher Scientific Glossary Aperture Diame
20. N 20 Live Camera in UV Mode svessccrtarsinsrvnrsaesteapeauaeedvaensnaniaasivedtianrenteaenreen 26 Live Camera in Vis Mode sssccccceeesssesssceeeceeeseeseseeeeeeeeessesseeeeeeeeesseseaes 26 Camera Settings Available in Chemi UV and Vis Modes n SE settings Only Available in UV and Vis Modes ascceceenn 29 Chemiluminescence Chemi Mode esssssssssn 30 Camera Settings ccccccccscscsessscsesssssessssseessseeessseseseceuessseeesssesessnenesenenes 30 WSR AC TIVO Chemi ere ms 30 KEE eessen 33 Singe Image Vere T EEN 33 Pre set Exposure RITE RR 34 Ges SS orc TIRE eee EEN 35 Multiple Image ACQUE OR seeeee cas cencecac eneteasGascecaeaeanecarcasemedetesrocaieencenaee 36 Pre set Multiple Exposure Settings NENNEN 37 User defined Multiple Exposure Setting 38 Mage e 40 E E E E u SEU ME MED UE UE Bend 40 Mise ERES ETE T RR 40 IER 41 ENEE 42 WEE 42 REENEN EE 43 tege 43 SITT 43 ECTS Tu t 44 Zu NN 45 MET UE day adse A ET 45 Ultraviolet UV Mode 46 Camera dl LEE 46 EH 46 mage EI TTE TNR RR 46 Mich MS TTE E 47 Custom Exposure E 48 Sion Image ee EE 49 Mage WOON E 49 Display Sata EE 49 eg LATI s NE E T E 50 PING ELI ERN PRETI PTT 50 WEE 50 IT 51 Ee 51 SITT 91 Tur MING ERR RC 51 PO 51 BER AEE ANE AAE EAA EN E A EEE 51 MN TE silanes Tm 51 Visible Vis Mode sssssss 52 EE 52 Image Acquisition Transmitted Light EEN 52 Image Acquisition
21. Pre set or Custom exposure time select one of the Pre set exposure time buttons using the keypad 0 1s 0 1s 0 2s 0 3s 0 4s 0 5s 0 6s 0 7s 0 8s 0 9s Custom Acauire 1 Us 2 select the Acquire button to capture an image 53 MYECL Imager User Manual Thermo Fisher Scientific 54 Custom Exposure Time Image Tools MYECL Imager User Manual 3 Captured images are displayed on the screen and automatically stored in the 1 Gallery for further viewing and selection Note A Stop button appears over the keypad once the Acquire button is selected Image acquisition can be stopped by selecting the Stop button Select the Custom exposure button using the keypad Enter the desired time using the keypad numbers The m s box is used to input seconds and or minutes and is active by default box is gray in color For a custom exposure time of 1 second select the ms box to input milliseconds The shortest exposure time that can be entered is 10 milliseconds this appears as 10 in the ms box m s ms 1 2 3 4 5 6 7 8 9 Acquire 0 Cancel 3 Once a value is entered into the Custom time field the Acquire button will become active Select the Acquire button to capture an image Captured images are displayed on the screen and automatically stored in the Gallery for further viewing and selection Note A Stop button appears over the keypad once the Acquire button i
22. User Manual Thermo Fisher Scientific 8 Imager Front View with Sample Door Open White Door Peg Epi white Light Bars o Magnetic Door Latch Glass Sample Platform Transilluminator Figure 2 2 Imager view with the sample door open and the transilluminator extended MYECL Imager User Manual Thermo Fisher Scientific Imager Rear View Thermoelectric Camera Cooler Lifting Handle Computer Hard Power Cooling Switch and Fan Power Cord Connector Ethernet Network Connection Figure 2 3 Rear imager view 9 wvECL Imager User Manual Thermo Fisher Scientific Start Up IMPORTANT Allow the imager to equilibrate for 20 30 minutes after each initial hard power up This ensures that the camera is optimally cooled before image acquisition Hard StartUp To perform the Hard Start Up procedure 1 Turn on the hard power switch located at the bottom of the rear imager panel The hard power switch provides power to all of the imager components 2 Turn on the soft power switch on the front of the imager This switch activates the internal computer touchscreen and thermoelectric camera cooler 3 The computer boots up 4 The user is prompted to enter the current date time and time zone Upon completion the screen automatically moves to the default Chemi acquire Thermo zs zz SCIENTIFIC z Chemi UV S Vis g Gallery Date and Time d Date and Time Additional Clocks Internet Time Date Friday Fe
23. ager User Manual Thermo Fisher Scientific Intensity Display Zoom File Menu Image Info Export Print Delete 51 MYECL Imager User Manual oelect a point of interest on the acquired image to view the Intensity Display containing the pixel intensity and pixel coordinates of the corresponding region When multiple images of the same sample are acquired and displayed on the screen the pixel intensities of the same location can be viewed and compared between multiple images by selecting the corresponding image tabs A Zoom slider bar is located under acquired images for zooming in and out on the image The zoom level percentage is displayed next to the slider bar The File Menu consists of buttons for Image Info Export Print and Delete Image Info can be selected for any captured image This lists the acquisition information for the highlighted image select Export to save the highlighted image to a USB flash drive External flash drives can access the imager through three different ports on the right side of the imager Note Users may need to momentarily wait for the imager to detect the external drive once it is connected to the USB port The selected image is exported only to the first USB flash drive if there are more than two drives connected to the imager The Print button allows the highlighted image to be printed to a selected external printer A Print Preview window appears before printing begins The Delete
24. and environmental requirements Remove the outer packaging materials IMPORTANT Imager use outside of the workflows described in this manual may put the operator at risk of dangerous exposure to electrical shock or radiated emissions Do not use this instrument for any purposes or in any configurations not described in this manual Thermo Fisher Scientific 2 Contacting Us Imager Overview Included Accessories MYECL Imager User Manual For technical or service assistance on the MYECL Imager U S Customers 1 800 874 3723 International Customers 1 815 968 0747 European Customers 49 228 8502820 Asia Pacific Customers 81 3 5826 1659 Technical Support email for U S and international customers pierce ts thermofisher com Technical Support email for European customers perbio eurotech thermofisher com Technical Support email for Asia Pacific customers Info bid jp thermofisher com For more information about the Thermo Scientific Protein Biology product line please visit our website www thermoscientific com pierce The MYECL Imager is designed to be a useful tool for the researcher needing intuitive equipment and software with fast imaging capabilities Your imager purchase includes the following accessories each quantity 1 Thermo Scientific M mylmageAnalysis Software MYECL Imager User Manual CD Chemiluminescence exposure screen White light conversion screen UV exposure screen Imaging reference target
25. bruary 17 2012 gt l j M J Time 4 1K 9 54 36 AM y Change date and time tO Time zone UTC 06 00 Central Time US amp Canada Change time zone ECL li lager Daylight Saving Time begins on Sunday March 11 2012 at 2 00 AM The clock is set to go forward 1 hour at that time Notify me when the clock changes Get more time zone information online How do set the clock and time zone ji Thermo 10 MYECL Imager User Manual Thermo Fisher Scientific 11 Soft Start Up Shut Down Soft Shut Down Hard Shut Down MYECL Imager User Manual If the imager is powered down by the Soft Shut Down procedure then a Soft Start Up can be done using the front soft power switch The soft power switch is the blue lighted switch located just above the front door and below the touchscreen This switch allows the user power down the imager computer and touchscreen while maintaining power to the camera cooling unit This feature allows the user to power down the imager but once needed quickly restart the imager without having to wait for the camera temperature to re equilibrate 1 Turn on the front soft power switch The computer restarts and the touchscreen initiates with the default Chemi screen 2 Begin normal operation of the imager Note For extended periods of inactivity the imager can be hard powered off however for typical daily use optimal camera temperature and best image qualit
26. button permanently deletes the highlighted image Thermo Fisher Scientific 52 Visible Vis Mode SAA Camera Settings Image Acquisition Transmitted Light MYECL Imager User Manual Visible Vis mode is used for the capture of gel images using reflected and or transmitted light CAUTION Vis mode uses the UV lights from the transilluminator Long term exposure to UV light can be harmful Use the appropriate eye and skin PPE if there is any chance of exposure to the UV light Note Ihe transilluminator UV light is initially on for 5 minutes User input is requested for further use of the UV light If the Keep UV on button is not selected then the UV light will automatically turns off Note The live image is displayed with the exposure time used in the previous image acquisition in Vis mode unless the user changes the exposure time or changes the mode Vis mode uses the same functional Camera Settings as the UV mode 1 Select the Vis tab on the touchscreen Changing to Vis mode automatically turns on the UV lights within the transilluminator and the epi white lights 2 Optional Select the Live Camera button and enable the UV button if the live image is not displayed and the transmitted light is off 3 Open the imager door confirm the UV light is off pull out the transilluminator and install the white light conversion screen on top of the transilluminator 4 nsert and center the sample on top of the white light
27. color imaging Note The Display Saturation button is disabled when the Auto Contrast or Adjust Image functions are enabled Proceed with the steps for acquiring an image Invert image he Invert button is a useful tool for viewing faint gel bands This tool inverts the standard white background with black highlights to a black background with white highlights in Vis mode 55 MYECL Imager User Manual e 1 hd a e Ges BC zep TZEE Original Vis Image Inverted Vis Image Thermo Fisher Scientific 96 Auto Contrast Adjust Image Intensity Display Zoom File Menu Image Info Export MYECL Imager User Manual selecting the Auto Contrast button increases the contrast between most bands and the background to increase visibility This adjustment can be used only for touchscreen viewing or printing of the image The contrast adjustment is not saved with the image file Note Auto Contrast is disengaged when the Adjust Image button is selected select the Adjust Image button to modify the Black White and Gamma levels of the image to increase sample visibility This adjustment is only used for viewing or printing the image Image adjustments are NOT saved with the image file select the Reset button to restore Black White and Gamma to original levels Select the Close button to return to the previous screen Note Adjust Image is disengaged when the Auto Contrast button is selected oelect a point of inter
28. creen allows access to basic imager settings and functionality Tools 1 Select the Tools button to open the Tools screen Thermo s chemi E uv vis I een B Tools button Serial Number KR01211106 Date and Time Administrator Settings The Tools screen displays the serial number of the system and the current software version installed in the system Large buttons for Date and Time Printer Filters and Administrator Settings are displayed on the screen Select the appropriate button to enter setup for each item User Name Password 13 MYECL Imager User Manual Thermo Fisher Scientific 14 Date and Time MYECL Imager User Manual To correct the date time or time zone 1 Select the Date and Time button A Windows clock and calendar is displayed 2 Choose the correct settings as needed 3 Select the Apply button followed by the OK button to save the settings gf Date emi TN d x Date and Time Additional Clocks Internet Time A e 7 EN Date fa PR b Wednesday May 02 2012 KR01211106 KE x Sy ie wf Time N E vl 1 02 49 PM C y Change date and time Time zone Filters UTC 05 00 Eastern Time US amp Canada Change time zone Daylight Saving Time ends on Sunday November 04 2012 at 2 00 AM The clock is set to go back 1 hour at that time Notify me when the clack changes Get more time zone information online How do I set the cloc
29. d Extended UV on position Retracted UV off position Note The live image is displayed with the exposure time used in the previous image acquisition in UV mode unless the user changes the exposure time or changes the mode UV mode uses the same functional Camera Settings as the Chemi mode but adds the camera setting functions for Aperture UV light and Filter wheel position 1 Select the UV tab on the touchscreen 2 Optional Select the Live Camera button if the live image is not displayed Thermo Fisher Scientific Pre set Exposure Time 1 4 D 47 MYECL Imager User Manual Open the imager door and confirm that the UV light is off The epi white lights are activated once the door is opened Pull out the transilluminator and place the UV exposure screen clear on top of the transilluminator Insert and center the sample on top of the UV exposure screen clear using the live image display for reference Once the sample is centered close the imager door While viewing the live image adjust the necessary settings for Focus Digital Zoom Sensitivity Resolution Filter and Aperture to produce the desired image Note Camera Settings are only available with the live image display Optional Select the Live Camera button if the live image is not displayed select one of the pre set exposure time buttons using the touchscreen keypad The live image with the new exposure time is displayed If needed modify
30. d bias master creation by opening the door or selecting the Stop button stops the process and discards all acquired masters In this case previous dark and bias masters are used for subsequent image acquisition Thermo Fisher Scientific 8 Once the dark and bias image acquisition process is complete the following screen will appear 9 Select OK to return to the Administrator Settings screen Return Select Return to return to the previous Tools menu 24 MYECL Imager User Manual Thermo Fisher Scientific Imager Settings Live Camera Live Camera in MYECL Imager User Manual Chapter 3 Image Acquisition Upon powering up the imager the MYECL Imager welcome screen is displayed Welcome to Thermo Scientific myECL Imager Note When performing the Hard Start Up procedure the thermoelectric camera cooler is NOT at the ideal temperature of 25 C After powering up the imager allow 10 30 minutes for the camera to cool to the optimal temperature Enabling the Live Camera button displays the streaming image from the camera and allows access to Camera Settings Focus Aperture Resolution Sensitivity Filter and Digital Zoom Disabling the Live Camera button displays previously acquired images Opening the imager door enables Live Camera and turns on the epi white lights Refresh rate of live image display depends on the binning setting selected in Resolution Sensitivity greater resolution requires more time to display
31. e Consult Table 2 5 in Chapter 2 to confirm the filter choice is appropriate for the application Changing Filters The filter wheel allows for easy installation and removal by using threaded filters The filters are fragile so use caution when handling and installing or removing IMPORTANT When installing filters ensure that the filter threads are correctly aligned with the filter wheel threads Forcing a filter to screw in can result in cross threading and damage to the filter wheel 1 Turn off the system power 2 Open the imager door and locate the filter wheel at the rear of the cabinet Each filter position is labeled with a red position number Position 1 Permanently empty for chemiluminescent imaging Position 2 Orange filter default filter supplied with the imager Position 3 Open for additional filter Position 4 Open for additional filter 65 wvECL Imager User Manual Thermo Fisher Scientific 3 To install an emission filter insert the threaded side of the filter into an open port on the filter wheel Turn clockwise to screw the filter in place until hand tight 4 To remove an emission filter grasp the outer edge of the filter housing and turn counterclockwise to unscrew and remove 5 When filter installation removal is complete turn the system power on 6 Update the filter settings in the Tools menu See Chapter 2 Imager for directions on changing the filter settings 66 myECL Imager User Manual Thermo Fish
32. e current session exposure settings Note The Image Tools only function while viewing or printing the image Display Saturation Invert Auto Contrast and Adjust image adjustments are NOT saved with the image file In UV mode while viewing the live image or after capturing an image the Display Saturation button can be selected to highlight the image pixels that are considered to be saturated A red false color is superimposed on the pixels deemed to be Saturated Adjustment to the saturation can be done by adjusting the Aperture and or exposure time for subsequent images Shorter exposure times reduce the light exposure saturation 1 While viewing the live image display select the Display Saturation button under the time keypad 2 he screen displays the saturated areas of the image with a red false color 3 While viewing the live image display in Camera Settings use the Aperture slider bar to adjust the saturation by opening moving slider to left or closing moving slider to right the aperture Opening the aperture increases image brightness and saturation Closing the aperture decreases image brightness and saturation 4 If required the image exposure time may need to be increased or decreased to display a properly saturated live image Exposure time can be adjusted before image capture by using the number keypad 5 Select the Display Saturation button again to turn off the false color imaging Note The Display Saturation but
33. e lit round button located on the front imager panel Stylus Pen shaped device used to input commands on the touchscreen Transilluminator Sliding sample work platform containing the UV bulbs Ultraviolet UV mode Captures images using transmitted ultraviolet light Visible Vis mode Captures image using transmitted light and or reflected visible light Thermo Fisher Scientific thermoscientific com pierce 2013 Thermo Fisher Scientific Inc All rights reserved Windows Windows 7 Windows Vista Windows XP Excel PowerPoint and Word are registered trademarks of Microsoft Corporation SYBR and SYPRO are registered trademarks of Life Technologies Corporation Deep Purple is a trademark of GE Healthcare Limited UK Pentium is a registered trademark of Intel Corporation All other trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Life Science Research Belgium and Europe The Netherlands the Middle East and Africa Tel 076 50 31 880 Distributors Germany Tel 32 53 85 71 84 Tel 0228 9125650 France United Kingdom Tel 0 800 50 82 15 Tel 0800 252 185 2162390 2 Switzerland Tel 0800 56 31 40 Email perbio euromarketing thermofisher com thermoscientific com perbio For other regions visit thermoscientific com piercedistributors
34. ealtek HD Audio Manager Recover y amp Region and Language E RemoteApp and Desktop Connections B System ii Taskbar and Start Menu a Troubleshooting Si User Accounts Windows Firewall T Windows Update Reset Default Focus Position he default focus position can be reset or changed by selecting the Reset Default Focus Position button The focus position may need resetting if the current default focus position does not provide in focus images The administrator can control this position by going to Tools gt Administrator Settings gt Reset Default Focus Position and using the slider bar to focus in or out to achieve the required sharpness in Chemi UV and Vis modes Update Software This function installs and updates the acquisition software when a program update becomes available Change Username and This function allows the administrator to change the username and password for Password accessing the administrator settings 21 MyYECL Imager User Manual Thermo Fisher Scientific 22 Create Dark and Bias Images MYECL Imager User Manual Dark current is the thermal noise generated from a CCD charge coupled device This thermal noise is proportional to a power law of CCD temperature The bias noise is introduced as the analog signal is converted to a digital signal The MYECL Imager applies dark and bias noise compensation using dark and bias masters upon image acquisition This function allows the admi
35. ensitivity camera settings are available in Chemi mode Interactive Chemi Interactive Chemi is a patent pending interactive tool used to evaluate and optimize imaging exposure time before acquiring a Western blot image The Interactive Chemi button automatically calculates the exposure time of the Western blot with maximum dynamic range and minimal pixel saturation from a short exposure image The feature displays real time changes of the calculated Western blot image with adjusted exposure time and allows further exposure time refinement through user input 1 Select the Interactive Chemi button The imager acquires a 15 second exposure Vis W Gallery 6 e emi Live Display 2013 April 18 14 53 23 e Interactive e SE Chemi Interactive Chemi button ime SCIENTIF Note A Stop button appears over the keypad area once the Interactive Chemi button is selected Image acquisition can be stopped by selecting the Stop button 30 MYECL Imager User Manual Thermo Fisher Scientific 2 The imager calculates and displays the exposure time with maximum dynamic range and minimum pixel saturation The slider bar location and Time Display Input button correspond to the calculated exposure time Note Exposure time ranges from 1 second to 99 minutes 99 seconds If the 15 second sample image is saturated then the imager automatically sets the calculated exposure time to 5 seconds Thermo Chemi SCIENTIFIC Interact
36. er Scientific 67 MYECL Imager User Manual Appendix A Technical Specifications Imager Parameter Power Maximum Frequency Range AC Voltage Current Fuse Dimensions Weight Wavelength Range Camera Lens Filter Wheel Touchscreen Light Source Manufacturer opecification 120W 50 60Hz 100 240V 1 2A maximum dependent on supply voltage T4AL 250V 4A fuse 21 inches H x 12 25 inches W x 22 inches D 53 3cm H x 31 1cm W x 55 9cm D 65lbs 29 5kg Specific wavelengths between 370 700nm High resolution thermoelectrically regulated at 25 C 4 2MP digital camera with 16 bit dynamic range 50mm f 0 95 aperture 4 filter positions motorized 10 4 inch LCD screen 306nm trans UV epi white Thermo Fisher Scientific 3747 N Meridian Rd Rockford IL 61105 0117 USA Thermo Fisher Scientific MYECL Imager User Manual Appendix B Accessories Product No 62237T 62238T 62239 62240 62241 62242T 62243T 622441 62255 62258 622611 622671 EW84857 84858T 84859T 848601 Product Name MylmageAnalysis Software Orange Filter Green Filter Red Filter Blue Filter Chemiluminescence Exposure Screen White Light Conversion Screen UV Exposure Screen Transilluminator Bulb 365nm Transilluminator Bulb 306nm Touchscreen Stylus and Holder Imaging Reference Target One Year Extended Warranty Power Cord C13 Connector North America Power Cord C13 Connector Continental Europe Power Cord C13
37. est on the acquired image to view the Intensity Display containing the pixel intensity and pixel coordinates of the corresponding region When multiple images of the same sample are acquired and displayed on the screen the pixel intensities of the same location can be viewed and compared between multiple images by selecting the corresponding image tabs A Zoom slider bar is located under acquired images for zooming in and out on images The zoom level percentage is displayed next to the slider bar The File Menu consists of buttons for Image Info Export Print and Delete 68 6 Image Info Export Print Delete Image Info can be selected for any captured image This lists the acquisition information for the highlighted image oelect Export to save highlighted images to a USB flash drive External flash drives can access the imager through three different ports on the right side of the imager Thermo Fisher Scientific 57 Print Delete Image Gallery Acquisition Information MYECL Imager User Manual Note Users may need to momentarily wait for the imager to detect the external drive once it is connected to the USB port The selected image is exported only to the first USB flash drive if there are more than two drives connected to the imager The Print button allows the highlighted image to be printed to a selected external printer A Print Preview window will appear before printing begins The Delete button perma
38. f Images may not be recovered after deletion images may nat be recovered after deletion ud um Are you Sure you want to delete fhese images bh E Ave you Bate you wart to delete hese mages e ve No x No As Uv Ze 200 XC tt P xw wa pasia MMM at UN e 23 x NM TE 8 e _ re ERES ES ee ee RI H gt be ie Uv fa 200200 r 2024 es 0 Uv fa W e Ek eg i y marmre Sy Sree E SS SP 60 MyYECL Imager User Manual Thermo Fisher Scientific 61 Export The Export button allows users to copy the selected images to the first USB flash drive plugged into the imager 2 Chemi 10s 20130205 1339 tif 3 Chemi 1s 20130204 1142 tif 4 Chemi 1m59s 20130201 1232 tif 5 Chemi 2m1s 20130201 1234 tif 6 Chemi 10s 20130201 0959 tif Exporting image 1 D Gallery_17 Chemi 1m59s 20130201 1232 Chemi 1m59s Gallery Edit Info The Edit Info button allows users to create or change the User Project Notes fields of the acquisition information for the selected images File names cannot be changed using Edit Info from File Manager Unselect The Unselect button removes all of the selections in the File Manager Exit File Manager Selecting the Exit File Manager button returns to the previous Gallery mode screen MYECL Imager User Manual Thermo Fisher Scientific Chapter 4 Maintenance Cleaning the Imager IMPORTANT When using any cleaning products with the imager consult
39. hemi Image Auto Contrast Adjusted Chemi Image Adjust Image Select the Adjust Image button to modify the Black White and Gamma levels of the image to increase sample visibility This adjustment is only used for viewing or printing the image Image adjustments are NOT saved with the image file Image Adjustments Gamma ji White Black d Auto Adjust Contrast Image Display Saturation Invert oelect the Reset button to restore Black White and Gamma to original levels Select the Close button to return to the previous screen Note Adjust Image is disengaged when the Auto Contrast button is selected 42 myYECL Imager User Manual Thermo Fisher Scientific Intensity Display X Select a point of interest on the acquired image to view the Intensity Display containing the pixel intensity and pixel coordinates of the corresponding region When multiple images of the same sample are acquired and displayed on the screen the pixel intensities of the same location can be viewed and compared between multiple images by selecting the corresponding image tabs ns X m Zoom A Zoom slider bar is located under acquired images for zooming in and out on the image The zoom level percentage is displayed next to the slider bar File Menu me File Menu consists of buttons for Image Info Export Print and Delete E EN Image Info Export Print Delete 43 wYECL Imager User Manual Thermo Fisher Scientific 44
40. ientific A Thermo Scientific MYlmageAnalysis Software MYECL Imager User Manual The mylmageAnalysis Software is supplied with the MYECL Imager as a vital tool for extracting information from your captured images The analysis software features Indentify lanes and bands automatically View and compare up to six images simultaneously Open multiple image formats e g tif jpeg png etc Export images and data reports directly to Microsoft Excel Word and PowerPoint software for further analysis and presentations Analyze molecular weight determine the R and molecular weight of protein or nucleic acid bands using installed or custom added molecular weight markers Edit digital images image editing tools include contrast adjustment image rotation cropping de speckling false color application and text annotation Calculate sample purity automatically based on band and lane intensity Perform lane profile densitometry Quantitate bands or regions relatively or absolutely Acquired images are saved and exported as tif files which can be directly opened in the analysis software Thermo Fisher Scientific 5 Installation Guidelines Space Requirements Electrical Requirements A MYECL Imager User Manual Chapter 2 Imager IMPORTANT Review and implement guidelines for proper set up before installation Locate the imager away from water solvents corrosive materials strong magnetic fields
41. ion The search results are displayed in the Search Result window the results listings are sortable by field Thermo Fisher Scientific Search Results The Search Results button allows for viewing results from the previous search Results listings are sortable by field File Manager The File Manager button allows for selection of multiple image files for copying deleting exporting and editing image information Images selected in File Manager are highlighted in a light green color The File Manager button can be selected from the Thumbnail File List or Search Results screens Note File Manager in Search Results contains a Select All Unselect All button in place of an Unselect button Copy The Copy button allows users to copy the selected images from one or more gallery folders to the desired location Thermo se chem EEE uv vs T Gatery Epon mme Bet Dem 2555052222000 KEE III ben ELSA Y p E Bi ee tebe E E BE ens en nee e A X008 One on fee en e L 9 n wI Jem in J gt XD EE EEr Les R teens z 2 D E Delete The Delete button allows users to delete the selected images from one or more gallery folders Thermo Chemi gv vw CODY me Export GT Unselect Ean File Manager Se ee Drege Me ertery Pay 7 oo o ee 08 fo c te WEI 7340 Gumery MN wed The tolowing 14 mages are selected hem 105 D E
42. isplay Auto Adjust Saturation Invert Contrast Image ez Display Auto Adjust Saturation Invert Contrast Image Thermo Fisher Scientific If the image appears to be over or under saturated the image exposure time may need to be increased or decreased to acquire a properly saturated image Exposure time can be adjusted for subsequent image capture by using the number keypad 3 he Display Saturation button can be selected again to turn off the false color imaging Note The Display Saturation button is disabled when the Auto Contrast or Adjust Image functions are enabled Invert image The Invert button is a useful tool for viewing faint gel bands This tool inverts the standard black background with white highlights to a white background with black highlights or vice versa Original Chemi Image Inverted Chemi Image 1 In the active live image or acquired image select the Invert button under the time keypad 2 The inverted image can be reverted to the standard image by again selecting the Invert button 41 MECL Imager User Manual Thermo Fisher Scientific Auto Contrast Selecting the Auto Contrast button increases the contrast between the signal and the background to increase visibility This adjustment can only be used for viewing or printing the image The contrast adjustment is NOT saved with the image file Note Auto Contrast is disengaged when the Adjust Image button is selected Original C
43. ive Chemi 00m 25s demum Time Display Input button Exposure 3 Use the exposure time slider bar and updated image preview to obtain the desired exposure time Drag and release the slider bar to update the image preview Alternatively select the Time Display Input button to enter a custom exposure time that updates the image preview and slider bar location Interactive Chemi Note Once a number is selected the Enter button is enabled and the Cancel button becomes a Glear button Select Enter or the Time Display Input button to accept the exposure time 31 MYECL Imager User Manual Thermo Fisher Scientific ER MYECL Imager User Manual 4 Select the Reset Calculated Exposure button to return to the imager calculated exposure time Note The Invert button is a useful tool for viewing faint gel bands This tool inverts the standard black background with white highlights to a white background with black highlights The inverted image can be switched back to the standard image by again selecting the Invert button Note The Display Saturation button is automatically selected in Interactive Chemi to highlight the image pixels that are considered saturated a red false color is superimposed on the pixels deemed to be saturated Adjustment to the saturation can be done by adjusting the exposure time with the slider bar or Time Display Input button Select the Display Saturation button to turn off the false color imaging 5 Optional
44. k and time zone on Administrator Settings Thermo Fisher Scientific 15 MYECL Imager User Manual Printer To select a default printer for imager exporting 1 Select the Printer button A Printers and Other Devices screen is displayed 2 Choose the appropriate printer from the listed selections Plug and play printers automatically populate the list Select Add a Printer from the left column if the printer is not a local printer or plug and play type A network printer can also be selected as a default printer Note Consult your facility s IT department for assistance in setting up network printers eme Ss M E UV E Gallery Lt Devices and Printers i E m K K j Control Panel All Control Panel Items Devices and Printers e Search Devices and Printers Add a device Add a printer e Devices 4 23 Add Printer Ka ef j e Add Printer lt What type of printer do you want to install D Add a local printer Pr Use this option only if you dont have a USB printer Windows automatically installs USB printers when you plug them in gt Add a network wireless or Bluetooth printer Make sure that your computer is connected to the network or that your Bluetooth or wireless printer is turned on Lr Cancel Thermo Fisher Scientific 16 MYECL Imager User Manual Filters Ihe imager has four default filter positions Position 1 No
45. loth or paper towel with cleaning solution e g general purpose cleaner isopropanol or 70 ethanol Wipe the surface of the transilluminator Note To ensure uniform illumination replace all six UV transilluminator bulbs even if only one bulb fails 1 Shut down the imager and unplug the power cord 2 Open the imager door and completely slide out the transilluminator Ensure the transilluminator is cool to touch 3 Loosen the four thumbscrews on the transilluminator coverplate 4 Lift the coverplate to expose the bulbs Use caution to avoid damaging the electrical cord 5 Remove the transilluminator bulbs by rotating 90 counterclockwise until the pins align with the bulb socket A click is heard when the bulb pins are aligned Lift the bulb up and out of the socket 6 Insert the new transilluminator bulbs by sliding pins into the bulb socket and rotating 90 clockwise A click will be heard when the bulb pins are set 7 Repeat Steps 5 and 6 for the remaining five bulbs 8 Replace all necessary components of the transilluminator and avoid damaging the electrical cord Replace and hand tighten the four thumbscrews 9 Push the transilluminator back into the imager 10 Plug the imager power cord back in and turn the system power on Thermo Fisher Scientific Emission Filters variety of emission filters can be used in the imager The appropriate filter choice is dependent on the emission wavelength of the sampl
46. nce a number is selected the lower Cancel button becomes a Clear button Use this to remove entered numbers Note Selecting the Cancel button returns the custom exposure time keypad to the pre set exposure time keypad 4 Select the Acquire button to capture the image 5 he image displays in a tab on the touchscreen and is saved to the Gallery Note A Stop button appears over the keypad once the Acquire button is selected Image acquisition can be stopped by selecting the Stop button Note Up to six recent images can be displayed on the touchscreen 35 MYECL Imager User Manual Thermo Fisher Scientific Multiple Image Acquisition Thermo zz chemi ERE UV Vis ES Gallery o0 Multi Exposure Settings X Q Interactive Pre set C Chemi Tap the button to capture images amera Exposure 1 2 3 4 5 Total H a SES s s Pre set2 10 30 1m 5m 10m 16m40s Pre set3 30 4m 5m 40m 45m 31m30s 10s 15s 30s Pre set4 1m 2m 5m 10m 20m 38m im 2m 5m Pre set5 1m 5m 10m 15m 30m 61m 10m 15m 30m User defined Edit Tap green button to capture tap edit button to update Clear ih Cuisten Exposure 1 2 3 4 5 Total s uem 10ms 20ms 30ms 40ms 100ms Camera Settings A Display Auto Adjust em Saturation Invert Contrast Image 1 Select the Chemi tab 2 Optional Select the Live Camera button if the live image
47. nently deletes the highlighted image The image gallery allows the user to view and open previously captured images Information for each captured image is displayed under the Browse button When an image is selected in the Gallery screen File Name User Project Notes Exposure Date Time Resolution Filter and Aperture information is populated in the Acquisition Information table Select Image Using Browse Button File Name Image title imager generated or user defined User Person using the wECL Imager user defined Project Name of project user defined Notes Relevant notes pertaining to image or experiment user defined Thermo Fisher Scientific Exposure Chosen exposure time for image Date Time Date and Time image was taken Resolution Binning used for image acquisition 1x1 highest resolution 8x8 lowest resolution Temperature CCD Temperature when image was taken Filter Emission Filter used for image acquisition Aperture Aperture change applied when image was taken Image Viewing 1 Select the Gallery tab at the top of the touchscreen 2 Click on the Browse button to open the gallery emm Thermo T Chemi Ld UV Wa Vis EF Galery 6 t Browse Acquisition informaton Select Image Using Browse Button IH ae Thumbnail Thumbnail displays previously captured images Images are separated into sections of days weeks months and years You can use the two right middle arrow buttons or the vertical scrollbar
48. nistrator to create new dark and bias master files Dark and bias masters need to be updated only when strong CCD noise appears on acquired images It takes 14 hours to create all of the dark and bias masters in the system therefore creation of new dark and bias masters is best accomplished by beginning the process at the end of the day and allowing the masters to be created overnight 1 Select the Tools button 2 Select the Administrator Settings button Enter the User Name and Password select the Login button 3 Select the Create Dark and Bias Images button The following screen is displayed Dark and bias masters created 2 13 2013 Dark and bias image collection requires approximately 14 hours 12 minutes 34 seconds Create bias masters Create dark masters Start Cancel 4 Select Start To create only dark or only bias masters choose the corresponding check box next to the desired selection Note Select the Cancel button to return to the Administrator Settings screen 5 Place the black sample screen on the transilluminator and close the door Thermo Fisher Scientific 23 MYECL Imager User Manual 6 Select OK to begin or Cancel to return to the Administrator Settings screen 7 Select OK The following screen will appear Note To avoid inaccurate dark and bias master creation the door must remain closed during the entire dark and bias image acquisition process Note Interruption of dark an
49. on in Chemi mode Saved ChemiV images can be displayed in Gallery mode along with the Chemi images 33 MYECL Imager User Manual Thermo Fisher Scientific 34 Pre set Exposure Time MYECL Imager User Manual 1 Select one of the pre set exposure time buttons on the touchscreen keypad The imager takes an image as soon as the exposure time button is selected Exposure Time 10s 1m 10m 15s 2m 15m 30s 5m 30m Multi 1h Custom 2 Captured images are displayed on the screen and automatically stored in the Gallery for further review and selection Note A Stop button appears over the keypad area once an exposure time button is selected Image acquisition can be stopped by selecting the Stop button Note Up to six recent images can be displayed on the touchscreen Thermo Fisher Scientific Custom Exposure Time 1 Select the Custom exposure button using the touchscreen keypad 2 Enter the desired time in milliseconds ms seconds s or minutes m using the touchscreen keypad numbers The m s box is used to input seconds and or minutes and is active by default box is gray in color For a custom exposure time of 1 second select the ms box to input milliseconds The shortest exposure time that can be entered is 10 milliseconds selecting 1 from the Keypad appears as 10 in the ms box 1 2 3 1 2 3 4 5 6 4 5 6 7 8 9 7 8 9 Acquire 0 Cancel Acquire 0 Clear 3 O
50. ppropriate time series of exposures 3 The images are automatically acquired 4 Captured images are displayed on the screen and stored in the Gallery for further viewing and selection Thermo Fisher Scientific User defined Multiple Exposure The User defined Multiple Exposure settings allow the user to set custom times in Settings situations where the pre set exposure times do not meet your needs User defined e Please click the red button to Edit an entry 1 Select the Multi button from the touchscreen number keypad 2 To create or edit the user defined exposure time settings select the Edit button to highlight the five Exposure fields 3 Choose one of the User option buttons to highlight the set of cells for user input 4 Using the pre set or custom exposure time keypad input the desired time into each cell The highlighted cell moves to the right as each time is input User defined Tap the Save button first then click the button to capture r7 38 MYECL Imager User Manual Thermo Fisher Scientific 5 The Total time is calculated and displayed as each new time is input into a cell 6 After all desired exposure times are entered select the Save button former Edit button in the top right corner of the table This saves the User time settings for further uses All five User time settings are entered and saved the same way 7 select the User button to automatically capture
51. r 1 Overview Dear Researcher We thank you for your investment in our Thermo Scientific M wECL M Imager We have worked to design a highly functional yet intuitive imager that will provide you with valuable images and data of high resolution and quality This manual has been written as a concise guide for the start up use and customization of your MYECL Imager unit and acquisition software Detailed sections covering the imager image acquisition file management maintenance and troubleshooting provide comprehensive information We encourage you to read the entire manual for a full understanding of the imager but a Quick Start guide is provided if you want to begin using your new imager immediately Thermo Scientific products are always at the forefront of scientific instrument design and we strive to design and produce instruments that can help you achieve your research goals Your feedback is a valuable tool that we use for future product development so please contact us if you have any questions comments or suggestions IMPORTANT Review and implement guidelines for proper set up before installation Locate the imager away from water solvents corrosive materials strong magnetic fields and vibration sources IMPORTANT Ihe imager weighs 65lbs 29 5kg Please use two people to lift it by grasping the bottom and the handle at the rear of the imager Using two people place the imager onto the benchtop given proper space electrical
52. r screen SYBR Gold Clear screen Orange 945 630 Ethidium Bromide Clear screen Hoechst Blue Clear screen Coomassie Blue White screen Imperial Wnite screent Red 620 645 SYPRO Ruby Clear screen Deep Purple Clear screen S With the MYECL Imager the orange filter is optimal for Hoechst Blue imaging t Coomassie Blue and Thermo Scientific Imperial Stains do not require the orange filter however these stains require the white light conversion screen UV and Vis modes use the orange filter in Position 2 by default 17 MYECL Imager User Manual Thermo Fisher Scientific Administrator Settings Selection of Administrator Settings displays the following screen requiring a User Name and Password Only the designated administrator should access this section User Name Password Users do not typically need to access Administrator Settings Only the facility administrator should have access to these functions through password access The default user name and password are myecl and 1234 respectively To avoid improper access the designated administrator needs to change this username and password once the imager is installed To change the password select the Change User Name Password button Thermo Chemi EMAa UV vis Gallery Oe ee O a l M o REM DEEN En Administrator Settings Set Image Set Default Other OS Gallery Location Focus Position Settings Allow Remote Change User Create Dark and Bias Images
53. re to the UV light 1 Wearing UV protective eyewear and clothing open the imager door and slide out the transilluminator 2 Place the UV exposure screen clear on the transilluminator and place the gel Sample on top of the screen IMPORTANT Use of the UV exposure screen clear protects the transilluminator from nicks and scratches as a result of excision 3 In UV mode select the UV button A message appears asking if the user wants to turn on the UV light with the door open Select Yes 4 The software then displays a warning message requiring the appropriate PPE and asks the user to pull out the white door peg Extended UV on position Retracted UV off position 5 Excise the appropriate bands from the gel sample Note As a safety precaution the UV light automatically turns off after five minutes With 30 seconds remaining a message appears on the touchscreen indicating that the UV light is turned off unless the Keep UV on button is selected Thermo Fisher Scientific 64 Cleaning the Transilluminator A Replacement of Transilluminator Bulbs MYECL Imager User Manual 6 When finished turn off the UV light by pushing in the white door peg Push the transilluminator back into the imager Alternatively push the transilluminator back into the imager and close the door which automatically pushes the white door peg back into place Ensure the transilluminator surface is cool Saturate a clean soft c
54. s touched Image acquisition can be stopped by touching the Stop button Note The Image Tools only function while viewing or printing the image Display saturation Invert Auto Contrast and Adjust Image adjustments are NOT saved with the image file Thermo Fisher Scientific Display Saturation In Vis mode while viewing the live image or after capturing an image the Display Saturation button can be selected to highlight live and captured pixels that are considered to be saturated A red false color is superimposed on the pixels deemed to be saturated Adjustment to the saturation can be done by adjusting the Aperture and or exposure time for subsequent images Shorter exposure times reduce the light exposure saturation jM While viewing the live image display select the Display Saturation button under the time keypad The screen displays the saturated areas of the image with a red false color Using the Aperture slider bar in Camera Settings adjust the saturation by opening moving slider to left or closing moving slider to right the aperture Opening the aperture increases image brightness and saturation Closing the aperture decreases image brightness and saturation If required the image exposure time may need to be increased or decreased to display a properly saturated live image Exposure time can be adjusted before image capture by using the number keypad oelect the Display Saturation button again to turn off the false
55. s appear after increasing the zoom level to center the image acquisition area Image acquisition with Digital Zoom results in capture of the zoomed image Resolution Sensitivity Icon for adjusting the camera image sensor s degree of response to light the number of camera pixels used to capture an image To enhance detection sensitivity the pixels or light capturing units of the CCD chip can be combined or binned The process of binning bundles multiple pixels which effectively increases the amount of light collected Figure 3 1illustrates the binning process using um pixels as examples for 1x1 2x2 and 3x3 binning settings Thermo Fisher Scientific 7ym 7um m gt Zum 7ym 7pm 144m Zum Y WA P Uj 7Tum 7ym Zum 21 777 Tum Ep A 21pm im fh Figure 3 1 The binning process The Resolution Sensitivity selection allows for choosing among five different sensitivities 1x1 far left setting 2x2 3x3 middle default setting 4x4 and 8x8 far right setting binning Selection of 1x1 binning provides minimum sensitivity maximum resolution while selection of 8x8 binning provides maximum sensitivity minimum resolution Table 3 1 provides multiple specifications related to the varied binning sizes Table 3 1 Binning specifications for sensitivity settings Binning Size PPI Pixel x Pixel Image size Image size Pixel size kb pixels inches x mm x mm um x um inch inches T 3 x 3 binning in 16 bit grayscale tiff image files
56. sure Project User and Resolution Thermo ze 1 Chemi EMAaA ov vs BE Gallery o0 Thumbnail Search Search Result Back to Gallery File Manager Images Directory D Gallery_21 a Gallery UY 200ms 20130415 1538 2013 04 15 4 38 PM 200ms Binning 3x3 Vis 200ms 20130415 1638 2013 04 15 4 38 PM 20ms Binning 3x3 UY 2s 20130324 1206 2013 03 24 12 06 PM Sacl BsrGl cut 2s cmv no Binning 3x3 Uv 600ms 20130324 1206 2013 03 24 12 06 PM 600ms Binning 3x3 UY 600ms BJO 32213 gels2 3 2013 03 22 5 14 PM 600ms Binning 3x3 UV 600ms BJO 32213 gels1 2 2013 03 22 5 10 PM 600ms Binning 3x3 UY 200ms 32113 gel2 3 2013 03 22 3 06 PM 200ms Binning 3x3 UY 200ms 32113 gel1 2 Uv 200ms green filter 2013 03 22 3 06 PM 2013 03 22 3 04 PM 200ms 200ms Binning 3x3 Binning 3x3 LI 300ms orange filter 2013 03 22 3 00 PM 300ms Binning 3x3 gst gstsyc gels 7 andv8 2min 32213 2013 03 22 1 54 PM Binning 3x3 LR Gallery gst gstsyc gels 7 and 8 30sec 32212 2013 03 22 1 51 PM Binning 3x3 gst gstsyc gels 5 and 6 2min 32213 4 2013 03 22 1 49 PM Binning 3x3 Open Chemi image The Search tab allows you to search through the full gallery Images can be searched by File Name Date Notes Project or User by entering the needed informat
57. ter of the lens opening that limits the amount of light entering the camera CCD Charge coupled device that converts a photoelectric Signal into electronic data CCD Camera Camera with CCD sensor used to capture moving and still images Chemiluminescence Chemi mode Captures image of sample emitted luminescent as well as sample image under epi white light Display Saturation Displays red painted image pixels if pixel intensity reaches a value near saturation Emission filter Optical glass filter mounted in the Filter Wheel used for filtering specific wavelengths of light Filter Wheel Automated wheel that can hold multiple filters in front of camera lens Gallery Holding space for captured images Hard Main Power Switch Main power rocker switch located on the rear imager panel Lens Optical device that controls light transmission Noise Imperfections in the image data Plug and play Ability of a computer system to automatically configure and operate other connected devices Remote Access Imager access by technical support to a remote location Reset Returns camera settings to the standard or home value 70 MYECL Imager User Manual Resolution Sharpness and clarity of an image defined by the number of camera pixels used to capture an image Sample screens The clear black and white panels placed over the transilluminator during image acquisition sensitivity CCD camera s degree of response to light Soft Power Switch Blu
58. the dS le S appropriate material safety data sheet MSDS to confirm compatibility with the anda oampte ocreens imager and proper usage of the cleaning product LCD Touchscreen Spray a soft cloth or paper towel with cleaning solution e g general purpose cleaner isopropanol or 70 ethanol Wipe the surface of the transilluminator the sample screens and the imager s exterior housing Cleaning the Touchscreen Use a pre moistened LCD screen cleaning tissue to clean the surface of the touchscreen IMPORTANT Use of ammonia based cleaners to clean the touchscreen is NOT recommended Do not spray cleaner directly onto the LCD screen IMPORTANT Do not press hard while cleaning the screen Do not leave excess cleaning solution on the LCD screen as this may cause long term damage Use of the Stylus The stylus is recommended for interacting with the touchscreen Use the tip of the stylus to tap the touchscreen for selection of menus icons and buttons Note Fingertips can be used for interaction with the touchscreen however the stylus is easier to use with gloved hands and prevents fingerprints on the screen 62 MYECL Imager User Manual Thermo Fisher Scientific 63 UV Transilluminator 2 AA Band Excision MYECL Imager User Manual CAUTION The UV transilluminator contains UV bulbs Long term exposure to UV light can be harmful Use the appropriate eye and skin personal protective equipment PPE if there is any chance of exposu
59. to move and view between the sections If the current gallery contains images from more than 40 image acquisitions the program automatically creates a new gallery folder and move to the new gallery Other galleries can be accessed using the up and down Gallery arrow buttons Thermo ow BH v gt oe em e ou EN 58 MYECL Imager User Manual Thermo Fisher Scientific 59 File List Search MYECL Imager User Manual Choose an image or series of images up to six images including ChemiV image by selecting the images on the touchscreen Selected images are highlighted in blue Double tap on an image or select the Back to Gallery button to return to the main image gallery screen Note Before selecting an image users can choose to view only the Chemi image or both the Chemi and ChemiV images of the same sample by selecting the Open ChemiV Image button at the bottom right of the Gallery screen This function is useful for establishing band locations in a Chemi image against known protein weight markers in a ChemiV image Note Select one ChemiM image to open all of the images associated with that specific multiple exposure Up to six images including ChemiV images can be selected and displayed at one time The File List button displays image information in a list format for ease of searching and sorting The list is sortable in eight different fields by selecting the appropriate column heading File Name Date Notes Mode Expo
60. ton is disabled when the Auto Contrast or Adjust Image functions are enabled 6 Proceed with the steps for acquiring an image Thermo Fisher Scientific 50 Invert Image The Invert button is a useful tool for viewing faint gel bands This tool inverts the Standard black background with white highlights to a white background with black highlights L d um pm em pm mm wm ben e a 12 Original UV Image Inverted UV Image 1 In the active live image select the Invert button from under the time keypad 2 Ihe inverted image can be switched back to the standard image by again selecting the Invert button Auto Contrast Selecting the Auto Contrast button increases the contrast between the signal and the background to increase visibility This adjustment can be used only for viewing or printing of the image The contrast adjustment is NOT saved with the image file Note Auto Contrast is disengaged when the Adjust Image button is selected Adjust Image Select the Adjust Image button to modify the Black White and Gamma levels of the image to increase sample visibility This adjustment is only used for viewing or printing the image Image adjustments are NOT saved with the image file oelect the Reset button to restore Black White and Gamma to original levels Select the Close button to return to the previous screen Note Adjust Image is disengaged when the Auto Contrast button is selected MYECL Im
61. y avoid hard powering on and off the imager The Soft Shut Down procedure uses only the front soft power switch 1 Save any current image analyses 2 safely remove any USB flash drives 3 Turn off the soft power switch 4 The computer and touchscreen shut down and turn off The fans continue running as needed he camera cooler remains powered and continue to maintain the camera at the optimal 25 C 5 he transilluminator and epi white lights turn off The Hard Shut Down procedure uses the hard power switch on the rear of the imager This turns off all power to the imager including the computer and camera Cooler 1 Follow the Soft Shut Down procedure 2 urn off the hard power switch at the rear of the imager Thermo Fisher Scientific USB Features The three external USB ports on the right side of the imager can be used for flash drives which can be connected through any of the USB ports for easy file management Note The Export button always exports files to the E drive which is automatically the first additional drive connected to the imager When connecting an external hard drive to the imager assign the external drive to a letter other than C D or E Touchscreen Upper USB Port Hard Power Button Lower USB Ports Figure 2 4 Location of lower USB ports 12 MYECL Imager User Manual Thermo Fisher Scientific Im ager Settings The Tools button in the top right corner of the acquisition software s

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MYECL™ Imager Manual - Thermo Fisher Scientific