SPIN-2 User`s Manual (Rev. 050816)
Contents
1. include Y Dedicated design for bilayer applications Independent cis trans dipoles Minimized magnetic flux at bilayer membrane Steel side panels for attachment of magnetic components Liquid crystal magnet positioning indicator Vibration free operation External isolated controller NS Ss S S S XS Rack mount controller available Warner Instruments A Harvard Apparatus Company Spin 2 Manual Rev 050816 4 NOMENCLATURE Text conventions To minimize the potential for confusion we have employed several text conventions which are specified below Since our goal is to provide clarity rather than complexity we welcome any feedback you may wish to provide Warner Instrument product numbers are presented using bold type References to separate components controls are specified using UNDERLINED SMALL CAPS References to specific controls are specified using NON UNDERLINED SMALL CAPS References to control settings are specified in italic type Special comments and warnings are presented in highlighted text Any other formatting should be apparent from context Vv Vv ON WV Warner Instruments A Harvard Apparatus Company Spin 2 Manual Rev 050816 5 INTRODUCTION Stirring of solutions in a bilayer cup and chamber has traditionally been achieved using either a commercial stirplate or a home made device Unfortunately commercial stirplates are not designed for use in a bilayer rig and home made devices can be inadequate du2. product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for those Harvard Apparatus products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of Harvard Apparatus products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive Harvard Apparatus will continue to monitor the application of the RoHS Directive to its products and will comply with any changes as they apply Special Collection Disposal Required Do Not Dispose Product with Municipal Waste Warner Instruments A Harvard Apparatus Company
3. stirbar chamber collisions or from magnetic effects Here we discuss strategies useful in minimizing or abolishing these effects Collisions Collisions between the stirbar and chamber will most likely be the largest amplitude noise artefact Fortunately this is also the easiest artefact to address Take a moment to review the setup procedure described on page 8 The strategy described there is to first center the stirbar in the front chamber over the front dipole then to adjust the position of the rear dipole and chamber so that the rear stirbar becomes well centered over the rear dipole We recognize that this procedure may seem cumbersome at first but the approach allows tremendous flexibility in using the stirplate with cups and chambers of many sizes and geometries Note that once the dipole separation has been set for a particular chamber only small subsequent adjustments should be necessary to align the stirbars between uses Magnetic induction Magnetic induction effects are more difficult to control As stated earlier this effect stems from the raw physics and simple geometry of the setup and cannot be engineered away However steps can be taken to reduce the amplitude of the artefact The dipoles within the stirplate are digitally controlled to minimize the amplitude of the magnetic field present within the bilayer membrane Moreover the resulting magnetic field vector also resides entirely within the plane of the membra
4. the chamber so that the axes are in Warner Instruments A Harvard Apparatus Company Spin 2 Manual Rev 050816 10 alignment However this approach is effective whenever only one side of the membrane is being stirred and can become intractable if stirring on both sides of the membrane are attempted The Spin 2 addresses this difficulty by providing two rotating dipoles one for each side of the bilayer membrane This approach allows each stirbar to remain centered within its respective well without influencing the motion of the other Careful positioning of the dipoles beneath the cup and chamber can eliminate noise artefacts due to stirbar collisions Another source of chamber noise is associated with the movement of fluid during solution exchange and will not be considered here Surrounding air Acoustic coupling from the surrounding environment can product a large transient artefact into your data Some researchers can find themselves turning the radio down or asking a coworker to not speak loudly next to the setup during a recording Acoustic noise can also come from a noisy device with the Faraday cage Most notable are stirrers and pumps While acoustic coupling from a radio is often transient noise from a pump or stirrer can be continuous However this effect is usually of a much smaller amplitude when compared to the mechanical artefact these devices can produce Magnetic noise Magnetic noise is not usually con
5. 9 Marne de NOE teer ee te etie ee einge eoe ute eie Art Pene teret teeter i 10 Nois abatement ss sosire 11 COLLISIONS Ado REED He ea STE AE EEE EERE EE CEREALS 11 Magnetic ind ction TETUR 11 BOW DII MH 11 APPENDIX EEEE AEE EE EE PU 12 SDPOCHICALIONS tH ee 12 Warranty and SOP VICG siscciscsciscicsssecsescacssseessandsseccesscassdenssed esesonessoetsoesasssones enssessedsFecesaaesecsnssvenedivnvessses 13 Warranty ces ead cates eck reete es er sows Ere a Lea a a Re eR a eee 13 NEVIO eee Raed Rate e BO NN ERRANT 13 CertificationS eisasssisesetssiksssenensene I rc 14 Spin 2 Manual Rev 050816 3 The Warner Spin 2 Bilayer Stirplate is a unique device designed to allow the simultaneous stirring of two solutions one on each side of a bilayer membrane The unit incorporates two counter rotating dipoles and the phase angle between the dipoles is digitally controlled to minimize the influence of a time varying magnetic flux on the headstage circuitry In addition the electronics and mechanical components are well shielded The unit sports an external controller which allows the user to adjust the rotation speed without opening the Faraday cage The mechanical components are designed to present virtually no vibration to the bilayer chamber The result is a quiet and versatile device that can allow the recording of electrophysiological signals while stirring Features of the Spin 2
6. Spin 2 Manual Rev 050816 Warner Instruments Dual Dipole Stirplate Model Spin 2 Warner Instruments 1125 Dixwell Avenue Hamden CT 06514 800 599 4203 203 776 0664 203 776 1278 fax CE Spin 2 Manual Rev 050816 Table of Contents DES GRIP TION eM 3 NOMENCLATURE 2 ooe es rae aoee Ero ae eE oe caseo eo UE aae ipea s oos ep io re aUe a re Fe epe o ebenen 4 AKS SEID LC EE UL T 4 INTRODUCTION rcp 5 CONTROL DESCRIPTION e sdcssecusstcecsuorchsceucstesosuadsecseutsesosscsssscevsiderecostuedeos stesdseatvesdon cosesencesese 6 Spin 2 bivio 6 Front patiel 4 eei dc d ur eee Re TE ee bad Ras EH eti de RE Pr A Riad deer RH Re este ae ee 6 Rear panels cien iiisteei eve teste EHE AEG EE Otto pe t D SPHERE april qu 6 Saia a EE NE 6 Front panel 35d ntn b dte petis dcn d m SaaS dia si tse 6 WT 6 O DA A B uem E 7 Stand alone StU e siess sivos 7 Rack mount setup SunStir setup s cosssssnssessnnennonsennnsensnnsensnnennnssnsnssessnnnnnsnennsnsnsnsssssnnsessnnesnssssssssessnnee 7 OPERATION ass 8 THEORETICAL CONSIDERATIONS es sossdetssocseccasesncchsssevstesestesertecosesetssosdstvesssecsossnecesacsoctets 9 SOURCES A A M 9 Electrical noises tne eb ene Ree TR a e a aa Se CRE 9 Mechanical NOISE ii t ie aeui t dune ide
7. an be difficult to control The fundamental issue is that the bilayer membrane represents an exquisitely sensitive transducer for mechanical movements and any vibrations presented to the chamber are received and amplified by the system Relevant sources include those from the chamber holder from within the chamber itself and from the surrounding air Chamber holder Vibrations associated with the chamber holder include the opening and closing of Faraday cage doors and the motion of the stirrer and other items placed on the isolation table Peristaltic pumps placed on the table within the cage are notorious noise sources as are many fluid control systems placed in direct contact with the bilayer chamber These problems are often addressed by placing the chamber on an isolated stand alone platform or stage which effectively separates the chamber from the offending noise source The mechanical components within the Spin 2 have been precision engineered using high mass materials and the device operates smoothly as a result Furthermore the top of the stirplate is somewhat isolated from its base and so can function as a stage Chamber Vibrations within the chamber are usually a result of collisions between a stirbar and the chamber wall These large amplitude artefacts are reasonably expected whenever a misalignment between the rotational axes of a stirbar and the stirplate exists This misalignment can be easily corrected by repositioning
8. e right is an external device used to power the STIRPLATE and to adjust the rotation speed of the dipoles Front panel The CONTROLLER front panel has an ON OFF SWITCH a ROTATION SPEED INDICATOR and a SPEED ADJUSTMENT KNOB The ON OFF SWITCH powers the stirring mechanism in the associated STIRPLATE SPEED ADJUSTMENT is via a continuously variable knob used to set the rotation speed of the dipoles in the STIRPLATE Slow and fast speed positions are indicated A GREEN LED indicates power on status as well as the relative rotational speed of the dipoles The faster the rotational speed the more rapidly the LED blinks Rear panel Several attachment points are located on the rear of the control unit These include a 6 pin DIN connector for connecting the STIRPLATE to the CONTROLLER a green grounding plug and a power input jack Stirplate Front panel The stirplate has a single control located on the front panel used to adjust the relative separation between the rotating dipoles Minimum separation is 0 4 in Top panel The top panel has a liquid crystal window to reveal the position and rotation of the magnets Two steel strips are located on either side of the liquid window under the overlay for attachment of magnetic supports Warner Instruments A Harvard Apparatus Company Spin 2 Manual Rev 050816 7 SETUP Stand alone setup 1 2 6 Place the Spin 2 CONTROLLER in a convenient loca
9. e to the limited engineering resources often available in a biochemistry lab In either case the most common design for the resulting stirplate is that of a single rotating magnetic dipole being presented to the bilayer chamber A result of this approach is that the chamber must be repositioned if the stirbar is moved from the cis or trans well to the other side More importantly it is virtually impossible to simultaneously stir both wells since the respective stirbars will be drawn to the common rotational axis defined by the stirplate magnet The resulting collisions between stirbars and the bilayer cup introduces a large noise artefact into the acquired data A second obstacle is associated with the interaction of a rotating magnetic dipole with the headstage circuitry The time varying magnetic flux through this closed loop generates an electromotive force resulting in the appearance of a sinusoidal current artefact in the data Many researchers avoid these problems by not stirring while recording which is often undesirable The Spin 2 has been specifically designed to address these difficulties and is the only apparatus commercially available to provide an integrated solution to these issues Several design strategies have been implemented in the Spin 2 Most notable is the presence of dual rotating dipoles one each for the cis and trans wells This approach allows the stirbar within each well to be independently manipulated which abo
10. lishes cup stirbar collisions The relative separation between dipoles is adjustable allowing the apparatus to be used with bilayer cups and chambers of various sizes Second the rotation phase and speed of the two spinning dipoles is digitally controlled This approach allows the Spin 2 to present the smallest possible magnetic flux to the bilayer membrane resulting in a reduced induction current in the headstage circuit A liquid crystal display on the top of the stirplate allows the researcher to view the rotating dipoles in real time Third the mechanical assembly is very quiet This feature introduces the possibility of placing the bilayer chamber directly onto the stirplate during use Most researchers currently place their chambers on an independent support stage which de couples the chamber from the mechanically noisy stirring mechanism The magnetic strength of the Spin 2 dipoles permits the use of either strategy to minimize noise in their setup Finally the apparatus is electrically isolated and well shielded allowing the rotation speed controller to remain outside of the Faraday cage during use Warner Instruments A Harvard Apparatus Company Spin 2 Manual Rev 050816 6 CONTROL DESCRIPTION The Spin 2 has two control points A DIPOLE POSITIONING KNOB on the front of the STIRPLATE used to adjust the relative separation of the dipoles and an external CONTROLLER Spin 2 Controller The Spin 2 CONTROLLER pictured to th
11. ls and workmanship for a period of one year from the date of shipment If a failure occurs within this period we will either repair or replace the faulty component s This warranty does not cover failure or damage caused by physical abuse or electrical stress inputs exceeding specified limits In the event that repairs are necessary shipping charges to the factory are the customer s responsibility Return charges will be paid by Warner Instruments Service We recommend that all questions regarding service be referred to our Technical Support Department Normal business hours are 8 30 AM to 5 00 PM EST Monday through Friday Our offices are located at 1125 Dixwell Avenue Hamden CT 06514 We can be reached by phone at 800 599 4203 or 203 776 0664 Our fax number is 203 776 1278 We can be reached by e mail at support warneronline com or through the web at http www warneronline com Warner Instruments A Harvard Apparatus Company Spin 2 Manual Rev 050816 14 Certifications Declaration of Conformity CE MARKING EMC Application of Council Directive 89 336 EEC Standards To Which Conformity EN55022 Class A Is Declared EN61000 3 2 EN61000 3 3 EN50082 1 1992 EN61000 4 2 EN61000 4 3 ENV50204 EN610000 4 4 EN610000 4 8 EN610000 4 11 Manufacturer s Name Warner Instruments LLC Manufacturer s Address 1125 Dixwell Avenue Hamden CT 06514 Tel 203 776 0664 Equipment Description Stirplate Eq
12. ne While this design reduces the amplitude of the induced current further reduction can be achieved by 1 reducing the rotation speed of the stirplate and 2 increasing the separation between the cup chamber and the stirplate Conclusions We hope this discussion has given you a deeper understanding regarding several issues associated with the process of stirring while recording We also hope that this discussion has provided you with the tools necessary to address these extra stirplate issues Warner Instruments A Harvard Apparatus Company Spin 2 Manual Rev 050816 APPENDIX Specifications Power Requirements Source DC connector Controls Controller Stirplate Display Controller Stirplate Speed range Rotor Synchronization Position adjustment range center to center Dimensions Controller Stirplate Weight Controller Stirplate 12 12VDC 11 to 17 VDC at 100 mA Wall mount DC power supply 2 1 mm power jack pos to inside pin Power On Off switch Speed rotary control Position adjust rotary LED flashes once per complete rotation Magnetic field display passive LCD 300 to 600 RPM Counter rotating 0 4 to 2 5 inches 5 4 x 0 5 x 2 0 in Wx D x H 5 5 x 8 0 x 2 3 in W x Dx H 0 5 Ib 3 0 Ib Warner Instruments A Harvard Apparatus Company Spin 2 Manual Rev 050816 13 Warranty and service Warranty The Spin 2 is covered by our Warranty to be free from defects in materia
13. rd Apparatus Company Spin 2 Manual Rev 050816 oo OPERATION The Spin 2 stirplate contains two rotating dipoles One dipole is positionally fixed relative to the stirplate case while the other is adjustable using the POSITION ADJUST control We will refer to the fixed dipole as the front dipole and the adjustable dipole as the rear dipole The general setup procedure is to first orient one well in the bilayer chamber to the front dipole and then to dial in the rear dipole to the other well In general a mechanical noise artefact is usually generated by collisions between a rotating stirbar and the cup Since the Spin 2 is designed to independently control two stirbars one in each well it is critical that all stirbars are optimally centered within their respective wells 1 Begin by assembling your cup and chamber Apply lipids add solutions and agar bridges 2 Place a single stirbar into the chamber s front well and place the chamber on top of the stirplate 3 Turn the stirplate on and position the chamber so that the stirbar rotates quietly within the well and on top of the front dipole Move the rear dipole away from the front dipole if needed 4 Now without moving the chamber place a second stirbar into the rear well and begin moving the rear dipole into position 5 Continue to adjust the position of the rear dipole until the rear stirbar rotates quietly on top of the rear dipole 6 Once the magnet
14. s within both wells have been positioned turn the stirplate off insert your electrodes and continue with membrane formation 7 Check for mechanical artefacts by turning on the Spin 2 once a stable membrane is formed Make final positional adjustments as necessary Warner Instruments A Harvard Apparatus Company Spin 2 Manual Rev 050816 9 THEORETICAL CONSIDERATIONS Sources In a bilayer stirplate there are three possible sources of noise electrical mechanical and magnetic Electric sources include the motor used to drive the rotation of the dipoles and the power control wires entering the Faraday cage Mechanical sources include the mechanism providing the rotation to the dipoles and collisions between the stirbars and the chamber The interaction of the rotating magnetic field with the headstage electrodes represents the primary magnetic noise source in a stirplate Electrical noise Electric noise sources are well controlled in the Spin 2 The stirplate enclosure as well as the associated connecting cable are internally shielded which effectively makes the inside of the stirplate topologically outside the Faraday cage In addition the drive motors are of the dual bearing DC type which provides for very quiet operation This approach allows the Spin 2 to be adjusted using an external controller with no electrical impact on the Faraday cage contents Mechanical noise Mechanical artefacts are usually a stirplate bane and c
15. sidered to be a problem since most researchers avoid stirring while recording Nevertheless magnetic effects remain the only artefact which cannot be eliminated by the careful design of a stirplate In general a time varying field produced by a rotating magnetic dipole induces a small but detectable sinusoidal current in any current loops present In the case of a bilayer setup this loop would be the headstage electrodes While magnetic artefacts cannot be eliminated their effects can be reduced by 1 decreasing the strength of the magnetic field 2 slowing the rate of change of the magnetic flux spin rate or 3 by altering the geometry of the wire loop within the magnetic field The Spin 2 uses highly focused magnets to reduce the strength of the magnetic field in the vicinity of the electrodes and the variable speed control allows the user slow the rate of change of the magnetic flux Warner Instruments A Harvard Apparatus Company Spin 2 Manual Rev 050816 11 Noise abatement The above discussion regarding various noise sources should make it apparent that a critical understanding of the principals involved can contribute immensely to the successful use of a stirplate while recording Since the Spin 2 has been designed specifically for this purpose care has been taken to abolish or minimize noise contributions from within the stirplate itself As a result most noise artefacts a user will experience will be from either
16. tion near your setup Do not place it inside the Faraday cage Place the STIRPLATE within your Faraday cage and route the attached connector cable to the CONTROLLER The cable carries its own internal shielding and so no additional steps need be taken to shield the unit Connect the two units by attaching the 5 pin DIN connector to the rear of the CONTROLLER Complete the ground circuit by connecting the CONTROLLER GROUND the green banana jack on the rear panel to your amplifier or cage ground otherwise known as the setup common ground Plug the power supply into a convenient outlet The Spin 2 is now ready to use Rack mount setup SunStir setup l 2 7 Place the SunStir RACK MOUNT CONTROLLER into your rack Place the STIRPLATE within your Faraday cage and route the attached connector cable to the CONTROLLER The cable carries its own internal shielding and so no additional steps need be taken to shield the unit Connect the STIRPLATE to the CONTROLLER by attaching the 5 pin DIN connector to the rear of the CONTROLLER If using the Sun 1 Halogen lamp make a similar connection from the lamp to the rear of the CONTROLLER Complete the ground circuit by connecting the CONTROLLER GROUND the green banana jack on the rear panel to your amplifier or cage ground otherwise known as the setup common ground Plug the SunStir into a convenient outlet The Spin 2 is now ready to use Warner Instruments A Harva
17. uipment Class ITE Class A Model Numbers SPIN 2 I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place Hamden Connecticut USA ah Fre Full Name Burton J Warner Signature Position President Warner Instruments A Harvard Apparatus Company Spin 2 Manual Rev 050816 15 Declaration of Conformity CE MARKING LVD Application of Council Directive 73 23 EEC Standards To Which Conformity Is EN61010 1 1993 Declared Manufacturer s Name Warner Instruments LLC Manufacturer s Address 1125 Dixwell Avenue Hamden CT 06514 Tel 203 776 0664 Equipment Description Stirplate Safety requirements for electrical equipment for measurement and laboratory use Equipment Class Class I Model Numbers SPIN 2 I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place Hamden Connecticut USA Signature xb Grr Full Name Burton J Warner Position President Warner Instruments A Harvard Apparatus Company Spin 2 Manual Rev 050816 16 WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers Harvard Apparatus is committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on
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