PLW20 – User manual - Prior Scientific, Inc.
Contents
1. 11 Click the Next button 12 The Scanning Hotel window will appear and the loader will automatically scan Hotel 1 pick up the well plate and move it to a safe position above stage SCANNING HOTEL When Scan compieted arm wil be retracted and ot tap of travel Then pren Mont to continue Fig 31 13 Click Next to continue to the Wait until Robot Stops Moving window WAIT UNTIL ROBOT STOPS MOVING Wart until monster comedete to tafe poniton above stage EXTEND Then press Naid to continue id ou Fig 32 14 Once the loader has finished moving the gripper arm click Next to continue 15 The Store Stage Setup Point window will appear vante Mowe ng Mowe STORE STAGE SETUP POINT g Ensure fst the stage is a the extreme laach right Grout CF fs movernent before continuing Using the arrow bes move ie Qricoer fp stage setup point Move Z to almost full extension auch Tat end of leew rail can ue be wenn Reter to user manus for further information Gam RIGHT Postion the mecroscope such thet well plate con be chopped J into stage Miast and je hah with left and beck dgis af l tho insert RETRACT ox Prem Mest to store the Sage postion end continue 5 x LEFT vv z Fig 33 16 Use the manual mover arrow buttons to the stage setup point NOTE The stage must be at its loading position As far back and as far right as it c2. Well plate hotel locking clasp Fig 08 Page 15 Locate dovetail on front of hotel under the plate dovetail on loader and lower rear of hotel down onto plate Fig 09 Fig 09 With the hotel in position move clasp to right Fig 10 to secure the hotel in place and activate hotel present switch Repeat for hotel 2 To Lock the clasp Fig 10 5 2 INSTALLING THE STAGE ONTO THE MICROSCOPE FITTING THE PROSCAN STAGE The Prior ProScan stage is supplied with the correct base plate to suit the microscope specified 1 Place the stage onto the microscope stage mount and attach using the fixing screws or clamping screw supplied Fig 11 2 Confirm that the ProScan controller unit is switched off before connecting the stage to the controller with the cable provided 3 The cable connections to the ProScan controller are located on the rear panel of the control box Before making any of these connections ensure that the ProScan controller is switched off Each connection is well labelled but great care should be taken not to try and connect your computer s serial port cable to the Z axis connector on the controller The RS232 connection from your computer should be made to the RS232 1 port on the controller For USB connection to your computer see separate section on this subject Mounting Cable to PLW20 Holes Front of Microscope Stage Locking Clamp Fig 11 5 3 POSITION OF THE MICROSCOPE Posi
3. 10 TROUBLESHOOTING 10 1 Error Codes SECTION 11 SECTION 12 Appendix 1 Appendix 2 Appendix 3 RETURNS AND REPAIRS APPENDIX How to Install The Barcode Reader How to Set Up And Use Prior Terminal PLW107 Nikon Microscope Fixing Kit 38 39 39 41 42 42 44 50 Section 1 1 1 SAFETY INFORMATION IMPORTANT SAFETY INFORMATION SAFETY WARNINGS Always observe the following safety precautions The PLW20 must only be connected to a power outlet which provides a protective earth Use only the mains cord supplied If this does not fit your power outlet contact your distributor for the correct power lead The unit is heavy and weighs 32kg Remove the microscope before transporting It should only be lifted by two people use side handle and lip of the rear cable entry area to lift Safely Always disconnect the equipment before moving This equipment is for use in moderate climates only NEVER use the equipment in damp or wet conditions Avoid excessive heat humidity dust amp vibration Do not use where the equipment may be subjected to dripping or splashing liquids This equipment contains no user serviceable parts Refer all repairs to qualified service personnel Keep fingers and hands clear of the PLW20 s moving parts when it is operating It is the user operators responsibility to ensure good laboratory practise is followed if well plates contain hazardous material SAFETY SYMBOLS Ret
4. Plate type Standard well plates with and without covers Plate change time of 28 seconds Input Voltage 100v to 240v Fuse rating for both fuses is T2A Communication Via USB section 3 UNPACKING THE SYSTEM 3 1 UNPACKING AND INSPECTING Carefully unpack the PLW20 and retain the packaging should the unit need to be returned If the PLW20 appears damaged in any way return it to your sales outlet in its original packaging No responsibility for damage arising from the use of non approved packaging will be accepted The packaging includes a pallet user appropriate pallet lifting equipment to transport the PLW20 in the packaging Ensure all items and accessories specified are present e PLW20 slide loader and microscope mounting plate e 10 position well plate hotel x2 e Installation Operating manual e Microscope Fitting kit e Cables mains amp USB If not contact your sales outlet or us at the address given in Section 12 Returns and Repairs Use only as specified by these operating instructions or the intrinsic protection provided by the unit may be impaired 3 2 SYSTEM INTEGRATOR The Prior PLW20 is designed and assessed to have no serious hazards However it is always used as a component within a complete microscopy system The PC terminal must be positioned close to the plate loader so that the PC operator has a clear unobstructed view of the plate loader This ensures that the PC operator will be aware
5. Test gt Start Soak Initiates an automatic process that cycles through all detected well plates moving them first to the stage and the reloads them to their original point The soak test will continue to load unload well plates until the Stop Soak is pressed at which point the system will stop when it has finished the current operation ie the load to stage step or the unload from stage step Soak Test gt Start Scan Soak Initiates an automatic process that first Scans the hotels before it cycles through all detected well plates moving them first to the stage and the reloads them to their original point The Scan Soak test will continue to load unload well plates until the Stop Scan Soak is pressed at which point the system will stop when it has finished the current operation ie the load to stage step or the unload from stage step Soak Test gt Rastor If this is checked then the stage will carry out a small rastor movement once the well plate been loaded onto the stage during the soak procedure Prior Wellplate Loader DisConnect Edit INI Fie SoakTest RedoSetup Wellplate Move Initialise Stage Single Step Enable Options gt RedoSetup Initiates the set up procedure should you need to redo the set up Options gt Initialise Forces loader to perform its auto initialisation sequence NOTE if the loader is in a compromised position the automatic re initialisation may result in a collision The user s
6. closing of the gripper jaws Y Axis Movement Gripper Arm Up amp Down Z Axis Movement Gripper Arm Extend amp Retract Out amp In Fast Stop Button X Axis Movement Gripper Arm Rotational left amp Right Fig 03 Page 12 G Axis Movement Gripper Jaws Open amp Close Fig 04 Page 13 section 5 INSTALLATION 5 1 INSTALLING YOUR SYSTEM Remove X axis assembly from packaging case and place on suitable bench Fig 05 Fig 05 Remove Y axis tower assembly from packaging and lower onto x axis assembly as shown Fig 06 Ensure mating faces are debris free and aligned correctly Align D connector on loader arm with the D connector on the loader base Fig 07 Remove Anti i Insert M5 x 16 socket rotation shipping button head screw into Insert M3 x 6 socket pan each of four positions and head screw to secure secure firmly screw Fig 06 Page 14 Once in place ensure that the bottom of the tower is flush with the base before securing with the four M5 screws and the single M3 screw Fig 06 amp 07 M5 Mounting holes M3 Mounting holes marked in Orange marked in Blue Fig 07 With Y axis tower installed remove anti rotation shipping screw Fig 06 Remove well plate hotels from packaging and fit onto loader plate Ensure locking clasp is positioned as shown below Fig 08 Hotel Present nn Hotel 1 Bay Switch b i gt D Hotel 2 Bay 4 i 4
7. cover of the drive box e Plug inthe barcode reader to its socket remember to make sure that the well plate loader is powered off The socket to connect the barcode reader is found on the reverse side of the drive box e Power on the well plate reader and the barcode reader will now be ready for use Fig 52 Appendix 2 HOW TO SET UP AND USE PRIOR TERMINAL Prior terminal can be found in the Downloads section of the Prior Website http www prioruk com downloadcentre dc_ software html Prior Terminal Set Up The installer will guide you through the steps required to install Prior Terminal on your computer WARNING This computer program is protected by copyright law and international treaties Unauthorized duplication or distribution of this program or any portion of it may result in severe civil or criminal penalties and will be prosecuted to the maximum extent possible under the law Click NEXT Prior Terminal _ Select Installation Folder The installer will install Prior Terminal to the following folder To install in this folder click Next To install to a different folder enter it below or click Browse Folder Ic Program Files x86 Prior Scientific Prior Terminal Install Prior Terminal for yourself or for anyone who uses this computer Everyone Just me Browse to a folder that you want to install the program into Confirm Installation The installer is ready to install Pri
8. of any person or obstructions in the vicinity of the plate loaders moving parts before starting the loader A red Fast Stop button can be found on the front of the Plate loader This can be used to instantly cut the power to the loader immediately halting any arm movements If the plate loader is to be operated remotely via a web link etc then adequate procedures should be put in place to either ensure that the operator can view the plate loader or ensure that no untrained personnel have access 3 3 PLW20 WHATS INSIDE THE BOX The PLW20 is securely packed into 3 sections inside of its shipping box Packing Section 1 2 x 10 Slot Hotels Packing Section 2 Plate Loader Tower Packing Section 3 X axis Assembly and base plate section 4 IDENTIFYING YOUR SYSTEM 4 1 THE PLW20 PLATE LOADER Z axis arm Barcode reader ontional Gripper Y axis tower Base Plate Hotel Rack 1 Hotel Rack 2 Apartment 10 Hotel Rack Z axis Linear Rail Gripper Arms Barcode Reader Fig 01 Apartment 1 Page 11 4 2 PLATE LOADER MOVEMENT AND AXIS IDENTIFICATION Plate loader movement is defined as X Y Z and G X axis being the rotational movement of the plate loader Y axis relates to the up and down movement of the loader arm Z axis Relates to the extend and retract movement of the extension on the gripper arm Finally G axis movement relates to the opening and
9. search for software Yes this tine only Co Yes now and every time connect a device Click Next to continue lt Back Fig 14 Select Install from a list or specific location advanced Found Mew Hardware Wizard This wizard helps you install software for USB Seral Converter If your hardware came with an installation CD J or floppy disk insert it now What do you want the wizard to do Install the software automatically Recommended Click Next to continue Fig 15 Browse to the location of FTDI USB Driver click NEXT Browse For Folder Select the folder that contains drivers for your hardware C2 Prior Prior Scientific O Prior Software 2 Beiter CD amd 4 iC i386 LD Headers E O PLX Drivers C2 ProScan2 OptiScan2 USB Driver 2 ProScan3 USB DRIVER C2 Prior Terminal amp O PriorScientific Image Grab To view any subfolders click a plus sign above Found New Hardware Wizard Please choose your search and installation options F Use the check boses below to limit or expand the default search which includes local paths and removable media The best driver found will be installed Search removable media floppy CD ROM Include this location in the search C Documents and Settings vw Browse Don t search will choose the driver to install Choose this option to select the device driver from a list Windows does not gua
10. setup position Click Yes to continue WellPlateLoader oy rt v i 2 Confirm Stage Setup Position Preus West to autornatically wansi weligiste hat to hotel Fig 38 26 Click Next on the Stage Setup Point Stored OK window to continue 27 The loader will now pick up the well plate from the stage and position it back into apartment 1 of hotel 1 and the following window will appear d _ TRANSFERRING WELLPLATE BACK TO HOTEL WAIT UNTIL WELLPLATE HACK IN HOTEL Thee geese Fieiah to oul Leg npn Heat ove Fig 39 28 Click Finish to complete the setup 29 Using the microscope locking blocks in position and gently rest the pads against the microscope to fix the microscope in position Section 7 OPERATION The Prior demo software can be used to run and test the installation and set up of the Well Plate Loader The functions of the application can be located in the menu bar Prior Weilplate Loader DisConnect Edit INIFile SoakTest Options ManualMove Stage Help Start Soak Wellplate Move System Status Start Scan Soak Stage Ho i5 Rastor Fig 40 Connect DisConnect This will establish a connection from the PC to the WellPlate Loader When the Loader is connected to the PC the button changes to DisConnect Edit Ini File Starts up notepad exe so that the setup configuration parameters can be manually edited Use with caution Soak
11. the loader and make sure that nothing is in a position where the loader can collide with it 1 Switch on the Well Plate Loader 2 Open Prior Well Plate Loader software Fig 19 3 Click Connect on the menu bar Prior Wellplate Loader Connect Edit INI File SoakTest Options Manual Move Stage Help gt zaz bip Wellplate Move System Status Spain Shui Hotel 2 a Se NOT CONNECTED STATE_UNKNOWN s Fig 19 4 Enter or confirm the Well Plate Loader Comm port number and the Prior Stage Controller Comm port Fig 20 The port address is automatically assigned by Windows but can be confirmed through the systems Device Manager Well Plate Loader Comm port Prior Stage Controller Comm port Enter port Enter port Cancel Cancel Fig 20 5 The manual mover screen will appear Fig 21 use this to move the loader to a position clear of the stage and about half way up the Y axis If a warning appears indicating that a well plate is in the gripper manually remove this well plate and continue Use the G button in the manual mover screen to open the gripper remember to place a hand under the well plate to catch it as the gripper arms open INFTIALISATION NOTE X Left amp Right Movement Y Up amp Down Movement Z Extend amp Retract Movement G Gripper Jaws Open amp Close Fig 21 Clo
12. the some of the most frequently used commands to a quick start button Right Click on and Quick Start Button and a dialogue box should appear to enter the command Button Value Button alue 1 Quick Start e Type in the command to be saved to the Quick Start Button In the above example the command is Version e Press OK e The button is now saved with this command Clicking this button will issue the command to the controller Appendix 3 PLW107 NIKON MICROSCOPE FIXING KIT To fix the Nikon Ti microscope to the base plate of the PLW20 Plate Loader you must use the PLW107 fixing kit Fixing Mase Brackets Screws M5 x 50 Slip Plate Screws This consists of the following items 1 off Slip Plate H3990 2 off Fixing Bracket Nikon Ti H3989 4 off M5x50mm Socket Cap Head Screws T238 4 off M5 x 20mm Socket Cap Head Screws T302 Installation Mount the fixing brackets onto the base plate of the plate loader using the appropriate screws Position the slip plate between the two fixing brackets M5 x 20 Screws Mount microscope onto the slip plate Use the Slip Plate to easily position the microscope to plate loader see page 30 of manual Once you are happy with the microscope position locks this in place using the 4 M5 x 50mm Screws
13. PRI cientifie PLW20 User manual PRIOR Scientific Prior Scientific Ltd Prior Scientific Inc Prior Scientific GmbH Prior Scientific KK Cambridge UK Rockland MA USA Jena Germany Tokyo Japan T 44 0 1223 881711 T 1 781 878 8442 T 49 0 3641 675 650 T 81 3 5652 8831 E uksales prior com E info prior com E jena prior com E info japan prior com Table of Contents Table of Contents SECTION 1 SAFETY INFORMATION 1 1 Important Safety Information 1 2 General Guidelines for Use Operation SECTION 2 SPECIFICATIONS 2 1 Specifications SECTION 3 UNPACKING THE SYSTEM 3 1 Unpacking and Inspection 3 2 System Integrator 3 3 PLW20 Whats in the Box SECTION 4 IDENTIFYING YOUR SYSTEM 4 1 The PLW20 Plate Loader 4 2 Plate Loader Movement and Axis Identification SECTION 5 INSTALLATION 5 1 Installing Your System 5 2 Installing the Stage onto a Microscope 5 3 Position of the Microscope 5 4 Connection SECTION 6 SETUP OF THE PLW20 6 1 Installing the USB COM port for PLW20 in Windows 7 6 2 Installing the USB COM port for PLW20 in Windows XP 6 3 The PLW20 Software Interface 6 4 Initialisation of PLW20 6 5 Setup of the PLW20 SECTION 7 OPERATION 7 1 Testing Set Up via Single Step Moves 7 2 Testing Set Up via Soak Test SECTION 8 ROUTINE MAINTENANCE ON CO N NN 10 11 13 13 16 16 17 18 18 18 21 22 24 32 34 35 37 SECTION 9 SOFTWARE INTEGRATION SECTION
14. ain these instructions The following symbols mean Warning read instructions to understand possible hazard Warning hazardous voltage Safety System Integrator The Prior PLW20 is designed and assessed to have no serious hazards However it is always used as a component within a complete microscopy system 12 GENERAL GUIDELINES FOR USE OPERATION The PLW20 forms part of an automated microscopy system which may be used in research or production laboratories It will pick up present sort and return plates to either of the two preloaded racks that are positioned at the front Whilst operating if a plate becomes misaligned do not attempt to correct this during the operation cycle Turn off the mains power before correcting plate position or removing any obstruction Reinitialise the system The PLW20 rotary arm may move unexpectedly under automatic control section 2 SPECIFICATIONS 2 1 SPECIFICATION This equipment is for indoor use and will meet its performance figures within an ambient temperature range 5 to 40 C with maximum relative humidity of 80 humidity deteriorating linearly to 50 above 312C Size 570mm deep x 850mm wide x 680m high Weight 57Kg gross includes Plate loader all accessories and packaging 32Kg Net plate loader fully assembled with accessories but no packaging The weight of the microscope is not included in these figures Capacity of 20 slides 2 racks of 10
15. an be 17 First extend the gripper arm to almost its maximum limits along the Z axis You should just be able to see the end of the Z axis linear rail End of Gripper Mechanism Fig 34 18 Move the gripper arm down the Y axis until it come to rest just above the motorised microscope stage NOTE Make sure that the stage clamp is in the open position Fig35 Stage Clamp Movement Fig 35 Page 30 19 Position the microscope such that the well plate can be dropped into the stage insert without the gripper arm clashing with the stage 20 Using the manual mover arrow keys carefully position the well plate inside the stage insert 21 Fine adjustment of the microscopes position maybe required now Fig 36 Fig 36 22 Position the microscope so that the well plate rests against the tabs located on the back and left side of the stage insert Fig 34 COCOREDTAES The well plate must be flush 8008808090 68 Well plate 29 il with tabs on the back and 000000000000 left hand faces of the stage 000000000000 606000000000 JM insert Fig 37 23 Using the manual mover arrow keys slowly adjust the height of the Y axis to carefully lower the well plate inside of the stage insert The well plate should be positioned so that it just rests on the insert face of the stage 24 Click Next to store the stage setup position and continue 25 The software will ask for confirmation of the stage
16. artments Stage bay i ae Pause NO FLATE ON ARM STAGE CLAMP OPEN Pause then Stop Well Plate Load Unload r Present sequence g Continue Load Unload seq uence STATE_IDLE F 1743s Last Err NOT INITIALISED Fig 43 3 Goto Options and check Single Step Enable Fig 41 The Do Single Step button will now appear 4 To transfer a well plate from the hotel to the stage position left click on the appropriate well plate in either Hotel 1 or Hotel 2 then left click on the Stage box and the loader will begin to load the well plate onto the stage 5 Using the Do Single Step button click through the sequence of steps until the plate is loaded onto the stage The Status will read Active will the loading procedure is in process 6 The single steps allows you to check the position of the plate at every stage of the load unload sequence If the plate does not load correctly go to Options Redo Setup and go through the set up procedure again 7 The sequence of events will end when the status reads back Idle 7 2 TESTING A SET UP VIA SOAK TEST 1 Add plates to the hotels and mount hotels onto the well plate loader base 2 Press Scan and the loader will automatically scan the hotel and record the location of any well plates present If a well plate is located it will be indicated as a green bar 3 Go to Opti
17. der and Plate Loader USB drivers and PCI card drivers Or 64bit software download for Window XP and Window 7 v8 4 35 Demonstration programs for ProScan OptiScan Slide Loader and Plate Loader USB drivers and PCI card drivers After installation of the software you can locate the WellPlateLoder demo program under the Prior Scientific Visual Basic folder of the Windows start menu Fig 13 Microsoft Silverlight Microsoft SQL Server 2005 Microsoft Visual Studio 2005 D Connect To NVIDIA Corporation Prior Scientific Control Panel DLL Visual Basic Default Programs Network Y Controller Demo k t PriorTerminal sor Y SlideLoader Demo J WellPlateLoader Demo Back Help and Support Fig 13 6 1 Installing the USB COM port for PLW20 in Windows 7 Plug in the USB cable to the PLW20 and the computer power on the PLW20 Windows 7 should automatically recognize the device and install the appropriate drivers 6 2 Installing the USB COM port for PLW20 in Windows XP Plug in the USB cable to the PLW20 and the computer power on the PLW20 When prompted select No not at this time then click NEXT Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CO or on the Windows Update Web site with your permission Read our privacy policy Can Windows connect to Windows Update to
18. des Note with some error messages it is necessary to reinitialize the loader before continuing NOT INITIALISED Plate Loader has not initialised Goto Options gt Initialise to begin initialisation process NOT SETUP Plate Loader has not been set up Goto gt Redo Setup to begin set up procedure STAGE NOT AT LOAD POSITION The motorised stage is not at the load Move the stage to the load position using position ready to accept the well plate either the joystick or Stage gt Move to Load function WELLPLATE CLAMP CLOSE Stage clamp is closed preventing the well Move the Stage Clamp to the open plate from successfully being loaded position See Fig 35 HOTEL IN USE REMOVED The software believes the Hotel is not Check to see if hotel is present present This could be due to Check to see if the sensor status changes The hotel being physically removed as you move the hotel location sensor during the process of transferring the from open to close If there s no change plate to or from the hotel in the sensor status the sensor has failed Or please contact Prior Scientific Failure of the hotel location sensor STAGE MOVED OF LOAD POINT The stage has moved from its loading Make sure the stage is returned to the position during transfer of the well plate load position either with the joystick or Stage gt Move to Load function COMMS ERROR Disconnect Connect Resullts from failure to establish Ch
19. eck power connections Check Device communication with the loader This Manager to make sure USB driver loaded could be due to either No Power to correctly Follow steps in Sections 6 1 or robot USB connection failed 6 2 to install USB driver AXIS STALLED The loader arm has stalled on either X Y Remove the physical obstruction if any or Z axis This could have been caused by present either Indicates problems with the encoder or A physical obstruction preventing motor please contact Prior Scientific movement Or It could also occur if some discontinuity exists between the true position of the loader and where the software actually thinks it is GRIPPER HOMING FAILED Gripper fails to home during initialisation Problem with the sensor please contact Prior Scientific WRONG GRIPPER SENSOR READING The sensor incorrectly detects if a well Possible causes could be plate is present or not Plate removed from hotel after a scan gt replace the plate in rack and restart scan Or Gripper malfunction contact Prior Scientific section 11 RETURNS AND REPAIRS Should you experience problems with your Plate Loading System and want to send it back for service warranty or otherwise a Return Material Authorisation RMA number must be obtained from the appropriate Prior Scientific office before returning any equipment For North and South America contact Prior Scientific Inc and for the rest of the world call Prio
20. he tower by LEFT RIGHT buttons Y Arm s height Move up and down the arm by UP DOWN buttons Z Arm s position Retract extract the arm by RETRACT EXTRACT buttons G Gripper cycles open close when pushing the G button The speed of movement along each axis can be controlled using the axis slider bar Moving the slider to the left slows the movement of the loader moving the slider to the right increases the speed of the loader Enable Disable Axis Fig 24 When making final adjustments to the Setup positions it is highly recommended to use the slowest speed to gain the most accurate control over the gripper arm position or use the Jog Move described below Jog Move INITIALISATION Fig 25 The Manual Move window Fig 25 allows you to move the loader along its X Y and Z axis using appropriate arrows keys The buttons relate to the following movements X Tower rotation axis Rotate the tower by LEFT RIGHT buttons Y Arm s height Move up and down the arm by UP DOWN buttons Z Arm s position Retract extract the arm by RETRACT EXTRACT buttons G Gripper cycles open close when pushing the G button The speed of movement along each axis can be controlled by changing the value of the encoder counts for each of the axis Fig 26 The larger the number the further the arm will move per click X Jog Distance encoder counts Enable Disable 10 Y Jog Distance encoder counts Axis Z Jog Distance encoder count
21. hould ensure that the loader is clear to move before continuing Options gt Single Step Enable If checked then the loader will enter single step mode whereby the user can step the loader through its cycle The Single Step button will appear on the main software interface This option is useful during installation to verify operation in a controlled manner Manual Move Initiates the manual move window This can be used to manual move the position of the loader arm Stage gt Move Stage to Load Moves the stage to the load position ready to receive the well plate Prior Wellplate Loader _ DisConnect Edit INIFe SoakTest Options Manual Move Move Stage to Load Wellplate Move Stage Hotel 2 Hotel 1 E Move Stage To Scan Fig 42 Stage gt Move Stage to Scan Moves the stage to the scan position ready to scan the well plate Help Contains useful information relating to the Prior Demo software 7 1 TESTING A SET UP VIA SINGLE STEP MOVES 1 Add plates to the hotels and mount hotels onto the well plate loader base 2 Press Scan and the loader will automatically scan the hotel and record the location of any well plates present If a well plate is located in an apartment it will be indicated as a green bar Prior Wellplate Loader eg U nscanne d DisConnect Edit INI File SoakTest Options Manual Move Stage Help Hotel Wetec Move System Status Stage Hotel 2 Hotel 1 of Empty Ap
22. it software or 64bit software depending on your Windows operating system Make sure the version number is v8 4 35 or above Fig 47 32bit software download for Window XP and Window 7 v8 4 35 Demonstration programs for ProScan OptiScan Slide Loader and Plate Loader USB drivers and PCI card drivers Or 64bit software download for Window XP and Window 7 v8 4 35 Demonstration programs for ProScan OptiScan Slide Loader and Plate Loader USB drivers and PCI card drivers 5 SSS s amp Neme A FTDL USE Driver Header PLX Drivers 2 ProScanz OptiScan2 U PraScen3 USE DRIVER ENDOFZTRAVEL 10 01 2042 1601 L Poor be HOTELSETUPI 01 01 2003 0100 I Prior Scientific E WOTELSETUPZ 01 01 2003 0101 E ProScan OptiScan2 USB Driver A ProScand USB DRIVER E STAGEPOSITIONL 01 01 2003 0100 L Prior Terminai EC STAGEPOSITION 01 01 2002 01 01 L QuickTime CWetPlsteLoader L Reference Assemblies i ma SASS Prior DII Help Dete modfisik 01 05 2012 1552 Compiled HTML Help file Low 120 KB Dete crested 01 05 2012 1552 Contents index Search Favorites Prior DLL Help 2 An introduction to the Prior DLL 2 Using the Prior DLL in Visual Basic 2 Using the Prior DLL in Visual C 2 Using the Prior DLL in C Q Prior DLL Classes The Encoder Class The Filter Class The LED Class The Piezo Class The Scan Class The Shuttle Class Fig 47 section 10 TROUBLESHOOTING 10 1 Error co
23. ng for the Baud Speed is 9600 however some software can change this so if you do not get a connection please try the other Baud speed setting If you have connected to the Prior Controller successfully it will be indicated in the Status Bar of Prior Terminal Working With Prior Terminal Once connected to the Prior controller Terminal can be used either to interrogate the controller current status or send commands direct to the controller A list of commands can be found in the back of the Prior Proscan III manual Type in the required command into the Input window of Prior Terminal and click Enter lt CR gt Some common commands are as follows General Commands Reports information about the peripherals currently connected to the controller DATE Reports the Instrument name version number and compiles time VERSION Reports the units software version number ERRORSTAT Reports any errors within the system RESET Resets the controller back to factory settings Any saved data will be lost Prior Terminal 2 5 Com ClearButtons ClearList DisConmect Macro Start Log OPTISCAN INFORMATION DRIVE CHIPS 00100 JOYSTICK IS DIGIPOT ONLY STAGE NONE FOCUS NORMAL FILTER_1 NONE FILTER_2 NONE SHUTTERS 000 An example of the command This responds back the information about the peripherals connected to the controller P EEE E e e Connected COM22 01 37 17 Quick Start Buttons It is possible to save
24. ons Soak Test gt Start Soak The loader will begin to load and unload the well plates in sequence until the Soak Test gt Stop Soak command is clicked 4 The Soak test can be paused or continued using the buttons on the screen 5 If the Soak Test Rastor is selected then during the Soak test the stage will also perform a rastor move Typical Screens Shots e Begining of a load Sequence loader Active Prior Wellplate Loader DisConnect Edit INI Fie SoakTest Options Manual Move Stage Help Wellplate Move System Status Stage 2 Continue Do Single Step Hotel 1 smn z z STATE_TXF_TOSTAGE 717435 Last Err NOT INITIALISED Fig 44 Plate selected and status now shows Active indicating the load process has started Status reading Plate on Arm indicating that the gripper has picked up the well plate e Well Plate loaded onto Stage loader Idle PEE Last Er NOT INTIALTIND Fig 45 Plate loaded to the stage position indicated by green bar in the Stage bay Status now reads Idle indicating the move is now complete e Well Plate scanned and Returned to Hotel Loader Idle ei Gk L UE ARE gt E e T Fig 46 Blue Bar in the Hotel 1 area indicates a plate that has successfully been loaded to the stage and returned back to the hotel Status reading Idle indicating m
25. or Terminal on your computer Click Next to start the installation Click NEXT Installation Complete Prior Terminal has been successfully installed Click Close to exit Please use Windows Update to check for any critical updates to the NET Framework Click CLOSE Page 46 Prior Terminal can be launched from the Start Menu ta Windows Photo Gallery iy Windows Update m YouSendit Alan Kidger Accessories cellSens Dimension Desktop Documents Dropbox EFACS Client Extras and Upgrades Pictures Music Games Hercules Favorites Maintenance Microsoft Office Recent Items Microsoft Silverlight Microsoft SQL Server 2005 Sampan Microsoft Visual Studio 2005 NVIDIA Corporation Prior Scier tr IC Network rm k k k k k k k k Connect To _ Visual basic Control Panel Controller Demo a A Default Programs Y SlideLoader Demo QuickTime Printers Skype Help and Support SolidWorks 2010 Back Start Search P Prior Terminal GUI Prior Terminal 2 5 Com ClearButtons Clearlist DisConnect Macro Start Log Connect COM Disconnect Port Main Terminal Input Window Quick Start Connected COM22 00 00 04 Configure Prior Terminal Using the COM Port Options menu choose the COM port and Baud Rate Speed The Default Setting for Baud Rate is 9600 If you do not know your COM port please check Windows Device Manager Default setti
26. ove is now complete section 8 ROUTINE MAINTENANCE SAFETY MAINTENANCE e Clean with a clean slightly dampened cloth Do not wet or allow moisture to penetrate the unit Do not use solvents on the plate loader as these may stain or damage the plastic covers e To ensure correct plate handing it is important that the interface between the plate hotels and the plate hotel mounting face is kept clean and free of debris e Remove dust and debris from the plate hotel mounting plate using a brush e Ifthe plate hotels should become soiled they can be cleaned using your laboratories standard cleaning procedure e The PLW20 only contains primary circuits with EMC filters If PAT testing apply DC flash tests only Avoid repeated application of such tests as this may damage insulation e SERVICING only to be undertaken by a competent person e This equipment contains no user serviceable parts Refer all repairs to qualified service personnel e Opening the product voids the warranty e Oil and residue removal Rinse the plate hotels in your laboratories designated cleaning solvent to remove the oil Refer to your health and safety procedures on using this solvent section 9 SOFTWARE INTEGRATION The information required for software integration is included in the software development kit This can be downloaded from the Prior Scientific website Please go to the Prior software download centre and download either the 32b
27. r Scientific Instruments Limited on the telephone numbers shown below Prior Scientific Instruments Ltd Prior Scientific Inc Prior Scientific GmbH 3 4 Fielding Ind Estate 80 Reservoir Park Drive Wildenbruchstr 15 Wilbraham Road Rockland D 07745 Fulbourn MA 02370 1062 Jena Cambridge USA GERMANY ENGLAND Tel 781 878 8442 Tel 49 0 3641 675 650 CB21 5ET Fax 781 878 8736 Fax 44 0 3641 675 651 Tel 01223 881711 email info prior com email jena prior com Fax 01223 881710 email uksales prior com Prior Scientific KK Kayabacho 3 Nagaoka Bldg 10F 2 7 10 Nihonbashi Kayabacho Chuo Ku Tokyo 103 0025 JAPAN Tel 81 0 3 5652 8831 Fax 81 0 3 5652 8832 email info japan prior com section 12 APPENDIX Appendix 1 HOW TO INSTALL THE BARCODE READER e Make sure the well plate loader is powered off and locate the barcode reader mounting bracket on the plate loader arm Barcode Reader a gt mm Mounting Bracket p ES Fig 48 e Fix the barcode reader to the mounting bracket with the 2 screws provided Barcode Reader LT Fixing Screws Fig 49 e Remove the cover from the Z axis drive box see Fig 50 to allow you to feed the barcode reader cable though the cable holes Fig 50 e Feed the barcode reader cable through the lower of the cable sockets through the drive box and out through the other side see Fig 51 Fig 51 e Once you have fed through the cable replace the
28. rantee that the driver you choose will be the best match for your hardware Fig 16 The FTDI USB Driver folder can be found in the Prior Scientific Prior Software folder Fig 16 This is part of the 32bit or 64bit software downloaded from the Prior website downloads section Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for USE Senal Converter Click Finish to close the wizard Click Finish Finish Cancel Fig 17 6 3 THE PLW20 SOFTWARE INTERFACE E Prior Wellplate Loader Continue Hotel 2 Apartments Status Bar Ny Se n a Qe T Pif RUN fea ES p Sa E JOUONS ManualMove Stage hep Eem System Status e NOT CONNECTED System Status Hotel 1 Apartments Hotel Scan Button Fig 18 6 4 INITIALISATION OF PLW20 Note A red Fast Stop button can be found on the front of the Plate loader This can be used to instantly cut the power to the loader immediately halting any arm movements This button must be release before power can be returned to the loader It is important for the plate loader to initialize to known datum points before you can proceed with the setup procedure The datum points are the limit switches which define the end points of the travel for the X Y and Z of the loader Before beginning the initialisation procedure remove any hotels well plate cassettes from
29. s Fig 26 NOTE A red Fast Stop button can be found on the front of the Plate loader This can be used to instantly cut the power to the loader immediately halting any arm movements This button must be release before power can be returned to the loader 1 Use the manual move buttons to step the gripper to the Hote 1 reference point 2 Check that the gripper arms are central in the insert flush with the base and the leading edges of the gripper paddles are flush with the set up tabs and the base of the paddles are just resting on the base tabs Fig 027 Fig 27 3 Click Next then the system will save the hotel setup position 4 The software will ask for confirmation of the Hotel setup position Click Yes WellPlateLoader 2 Confirm Hotel Setup Position _ HOTEL POSITION STORED OK 1 Se nate tatoos Fig 28 5 To continue Click Next Page 27 6 The loader will now move the gripper arm to a safe position ready for the next step of the setup EXTRACTING ARM as EXTEND Then press Went to comtinue Fig 29 7 Click Next to continue 8 The Fit Hotel window now appears FIT HOTEL Pace hotel en poste 1 wih a well plete ia apantment 1 Then press Noat to scan hotel Fig 30 9 Place a Hotel onto its bay in Position 1 of the well plate loaders main body Fig 01 10 Insert a well plate into apartment 1 of the Hotel 1 Fig 01
30. sing the manual move screen will bring up the following window WellPlateLoader Caution Stage will move to load position before auto initialisation starts Fig 22 6 Click OK to continue The stage will move to its Load position and the loader will automatically perform its initialisation Once the auto initialisation has been completed the Setup procedure can begin 6 5 SETUP OF THE PLW20 After the initialisation step has been completed you are required to complete a set up procedure If a set up has not already been completed then the manual mover screen will re appear It can be controlled in two ways Velocity Move or Jog Move Velocity Move EXTEND L Postion at dun arcs poor Craure robot arm de ponton in font of hoirh doat hall way 10 Robot mws can be mowed at cometant werd by holding down lelt maaie betine on he aporepeite ato i the Vetocity Mowe tab appaste Redeqang le barton ell cog the ats The speed can be varied by The shidir ba e Rabet cm moved by a podik armour by b mous dkkhg RIGHT oo he approeriate och arrow Ip The Jog Mowe tab opposte Jog Aane ln eecodk Courts can be speciied in the moult bos Prem Close te Sart suto intiainaton procedure DOWN RETRACT Fig 23 The Manual Move window Fig 23 allows you to move the loader along its X Y and Z axis using appropriate arrows keys The buttons relate to the following movements X Tower rotation axis Rotate t
31. tion the microscope and stage to approximately the correct position on the well plate base plate close to the plate loader The fine adjustment of the microscope position is carried out during the set up of the plate loader see Section 6 5 Setting up the PWL20 5 4 CONNECTION Rear view of loader shows cable entry points Fig 12 e Mains socket e 4way binder socket For cable from well plate on stage sensor e USB to PC Above the 4 Way binder socket are two fuse housings Fuse Housing Mains Connector On Off Switch 4 way binder socket Fig 12 Connect the Well Plate Loader to the PC with a standard USB cable Connect the Well Plate Loader to the Well Plate Stage using the cable from the well plate stage connected to the 4 way Binder socket 3 Connect Well Plate Stage to a Proscan III controller using the Prior Stage cable After checking that the Well Plate Loader and Proscan III controller switched off connect them to the mains supply section 6 SETUP OF THE PLW20 Before working with the PLW20 you must first install Prior software on your PC Visit Prior software download centre website and download either the 32bit software or 64bit software depending on your Windows operating system Make sure the software is version v8 4 35 or above http www prioruk com downloadcentre dc_software html 32bit software download for Window XP and Window 7 v8 4 35 Demonstration programs for ProScan OptiScan Slide Loa
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