Operation Manual FAIMS Data Analyzer
Contents
1. Modify Graph Modify Trace Appearance Reorder Traces Append Traces to Graph you can add other graphs into the display Remove from Graph removes data from the graph M kosca is Axis Properties Add Annotation Modify Trace Appearance opens a new panel There you can change m the colour the mode line dots markers etc of the displayed graphs Frame Quick Fit Modify Image Appearance is useable at the top displays opens a panel There you can change the colour type of the image e g to grays or the intensity to a concrete value Autoscale Axes scales the axes as before after you changed something by Axis Properties opens a panel There you can change the axis label the axis range the number of ticks etc Axis v Live Update Axis Auto Man Ticks Ticks and Grids Tick Options Anis Label Label Options Axis Range Mode Eategam f Linear Ais Font M Axis standoff Mirror Asis Ott Bold fa fault Itali Log Axis Thick 1 00 Axis offset jo m pus 7 Log Login Sie Auto Eine C Date Time Will change NN Ltlirie waves units Sick e Shadow Free Position f Dn ae of normal Color Distance from Margin Draw on Top of Traces 3 Ais mm Set All to Axis Color Distance Eategorn gap sy vs Bar aap E Asis Label m Tick Label um io IE Tio nnd Line To flip Help Cancel Ldetmdtme2. Add as much tags as you like To delete the last tag press Remove Tag button Confirm with Continue Button if all tags are right set or use Cancel to quit Creating a Reference A new window is shown Fig 7 it contains data according to your loaded measurement You can change the Sample Name and add Additional Note 13 Please fill in the right parameter at GC Parameter Confirm with the Continue button and choose a data folder and the save the name of the data liil GetSavelnfo Please complete the Inforamtion Sample Name Gori Esche 60 _1 Compensation Voltage V 0 29 Date Di 4 Mrz 2014 Additional Note GC Parameter Column Flow mL min 0 Purge Flow mL min 0 Split Flow mL min 0 Start Temp C 0 End Temp C 0 Heating Rate C min 0 Holdtime min 0 FAIMS Parameter Dispersion Field zz 60 Flow Rate L min 1 94 Hum 81 Fig 11 Editor to set GC Parameters Sensor Temp C 49 9 Loading a Reference file Press the Show button below the Int vs Time graph Notice You can display the Reference file even without having data overlayed by Overlay button The Overlay window appears Now press the Load Reference button A Load Dialog Window is opend choose the Reference you want to open confirm with Load The Reference file is append to the graph window and automatically the common GC and FAIMS parameter
3. Teck _ Hep _ Cancel Fig 5 Modifying the Axis properties Slider Intensity sliders The Intensity sliders change the intensity of the image for a better peak detection You can also do it manually by right click on the image and choose Modify Image Appearance Time vs Int sliders With this sliders you choose a constant compensation voltage row of the matrix which is extracted from the image and shown in the Time vs Intensity Display The compensation voltage you have chosen and the exact matrix s row for this voltage are displayed right below the image The horizontal line displays which one is extracted even when you have zoomed The slider is not on the right position if you have expanded the window CV vs Int sliders With this sliders you choose a constant time column of the matrix which is extracted from the image and shown in the Compensation Voltage vs Intensity Display The time you have chosen and the exact matrix s column for this time are displayed right above the image The vertical line displays which one is extracted even when you have zoomed The slider is not on the right position if you have expanded the window Buttons Pos Neg buttons With this buttons you can switch the recorded data between positive and negative ion mode Expand The expand button zoom in the area which you have to mark before by pressed left mouse button You can also expand manually by marking a
4. h ol d eft Fie Edit Data Analysis Macros Windows Graph Misc Help mouse button and 8 i PeaksGraph tempColPos vs ZeitO SEE move mouse right click within and choose 20 Expand eer gi Cursor A and B Drag Intensity a u 1 0 and drop one to the start and the other one to the end of your JE choosed graph 200 Horiz Expand 600 800 Vert Expand i Shrink a L pnt X E ge CHG a Horiz Shrink g 0 6 sali Vert Shrink 08 1 0 E Baseline amp Integration mE AreaFromMarquee 42 Choose between five IgnorePeaksWithinMarquee 0 200 400 different baselines and Mark start and end of the peak with cursor A and B IdentifyPkStartEndwithMarquee Time click Integrate to get Integrate relative to he Ar f th k tne ea of the pea y 0 I i El 02 ud m m 44 Area 0 3s f Then you have to pick a baseline for your integration Click the popup and choose between five baseline types t e y 0 the area between y 0 and the graph is calculated t e y an input area appears You set a constant line as baseline E g you set 0 this would be the same as y 0 e Constant line through cursor A the same as y but the program sets the constant line at the high of cursor A e Line between Cursor A and Cursor B a line between both cursors is drawn e Create own Baseline a cursor named C appear
5. Integrati Overla Show Clear A EI bad stad a ee New Window z Matrix s Col si 4 lil i Browse tempColPos Edit tempColPos Remove tempColPos 1000 1200 Time 5 Duplicate Trace Save Overlay Show j Clear nabse Replace Wave lay Show Clear Copy Display Command File Name F3 mit_1 4 Modify tempColPos Gustomize at Point 3 Mode gt Line Style gt Line Size 1 Markers Marker Size Color gt C Write in Report V Automatically Show Report 3 3 Bring Eo Front Send Ea Back Forward Backward Intensity a u M I Sho Graphic Name Intensity vs Time Set Time 706 638 s CV 0 341589 v Intensity 0 533311 a u Intensity a u voltage v Modify Contour Edit wave in a table Modify Image Fig 7 To display the raw data of a curve press the right mouse button near the curve IM Schumann Analytics FAIMS Data Analyzer 2 030514 Igor Pro 6 324 File Edit Data Analysis Macros Windows Table Misc Help Gizmo Zoom Fes m iol x Expand Axis Reset Times 0 J gt Matirs s Row D 12 0 142994 0 ieee tal 0 14214 0 146977 0 151906 55 0 140217 69 0 143574 83 0 141194 9B 0 150044 11 0 156102 124 Ra RA RE pull 0 149434 138 Time Bl 0 152745 154 ET B eripit c Ovetay Show Clear i EE 0 143574 165 0 143315 179 0 143803 193 0 154164 207 0 151402 22 0 151341 234 0 154653 248 0 152698 262 Z p Save Rel Oveoy S
6. are displayed in a subwindow on the right hand side To display the tags you have added during Create a Reference press Add Ref Tag all tags are automatically added to the graph To remove the Reference press the Remove Ref button If you want to load another Reference file use the Load Reference button you don t have to use the Remove Ref button before If you press the Close button the Reference is automatically removed Sub4 Sub3 7555s 603 3 GC Parameter Tabak without 0 34 V p Tabak without ref ColumnFlow mL min 7 Holdtime min 2 FAIMS Parameter Dispersionfield 30 FlowR ate L min 1 6 Humidity 7 1 Sensortemp C 51 2 sation Voltage V 0 342786 7 Matrix s Col 142 v N 13504s 1547 8s 3 A TRA y vi jy Ji Jj pa A 200 400 600 800 1000 1200 1400 1600 masta Waca a ie m ey a A y Overlay Positiv D 1200 1400 ime Tabak without 0 34V p Load Reference Add Ref Tag Close 1 0 1 2 Compensation voltage v E Fig 12 Comparison of an unknown substance with a reference spectra 14 3 3 Integrate Single Peak Choose Macros gt Integrate Single Peak A panel appears below the displayed graph Now you have to place cursor A at the start and cursor B at the end of the peak by drag and drop Therefore it is easier if you first expand the peak and then put the cursors on it M ark th e p e ak
7. e he printing output is lightly over printed with Igor related words To avoid these limitations or to create your own procedures you can purchase an IGOR Pro license to restore the full functionality 1 1 Preparing your data for analyse Before starting the FAIMS Data Analyzer it is necessary to convert the format of your data First create a txt file with the Review Process File from the Lonestar software Export data from Review Process File Vsn Initially load your Icbdf file choose File gt Export Data and click Ok in the windows panel Now the txt file is created close the Review Process File and start FAIMS Data Analyser Lonestar Process Review BusrEwertimsDsa aon ER OED Qa i cm ng Sensor Conditions 0 Weightings 100 reset Flow Rate Pressure Humidity z e Size Type Date Mo 5 542KB Text Documen t 14 11 20 Temperature Field Intensity m ror o 3us44n UOJ aje2say i System Temperatures Sensor Head T T v pep o9 99r il ONU Compensation Voltage V p pa e 1 1 0 5 1 1 49 Ion Current A U 1 Filter 0 2 0 Motherboard Alarm leve Alarm Reporting Pos Ambient 48 D My Computer Pump Voltage E lt e d Configuration co 1 oe File name Export Gor FAIMS Sensor Disconnecte Fig 1 Export the Icbdf file to a txt file 2 How to use FAIMS Data Analyser If it
8. Cose F3 mit_1_0 27V p F3 mit 1 0 51V p 0 Compensation voltage v Fig 9 Overlaying different chromatograms A click on the Close button will not delete the overlayed chromatograms it will only close the current window You can display the compared chromatograms again with a click on the Show button left under the chromatogram in the main window To clear these data from the temporary storage please press the Clear button left under the chromatogram in the main window 12 3 2 Creating and Using the Reference The save reference option is useful to create a library for chemicals you want to detect in an unknown sample Creating a Reference spectra First record a Continuous Mode file with a pure substance with the Lonestar software Then load it into the FAIMS Data Analyzer Choose a compensation voltage with the Time vs Int slider where your substance has a perfect peak Press the Overlay button left under the chromatogram window After that please press the Show button the and the current Int vs Time graphic is shown in a new window Here you have the ability to add tags on the graph 3 o m Add Subs Fig 10 Creating a Reference spectra Place the cursor initially placed bottom left at this place where you want to add the tag In the subwindow below enter a substance name Confirm with Add Subs button Your tag is shown in the graph window
9. Operation Manual FAIMS Data Analyzer File Edit Data Analysis Macros Windows Panel Misc Help W FAIMS Data Analyzer m Matrix s Row tutatrix s Row J Time 76 lt J Time 148 3 3 2 2 e gt EE S 1 ELLOS 1 15 E e E 4 g Ss 0 2 s 0 a i E L9 vi vi a 1 1 a a E E 8 2 2 3 3 200 400 600 s00 Mistrix s Column 200 400 600 200 Mistrix s Column Time s Cv 72 le Time s Cv 60 s i iM Show w Show i 0 0 ow e P 02 m m 1 04 s 06 2 0 0 8 1 0 1 2 oO 200 400 600 a00 D 200 400 600 800 um E 5 Show jr Show 7 d 02 m m i 0 4 1 E E 0 6 d pg 1 0 3 2 4 D 1 2 3 Clear ALL 3 2 4 D 1 2 3 Compensation voltage v Load next Wave Compensation voltage v Readu Fast data analysis and mstant report G peaks trapezoidal area templClolPos wy PkThreshold wy Pk StartEndThresh Vy Baseknots Vy Peake nots VW BaselineF it Intensity a u o s2s8 tats rm57137 107795 46 6 626 1 asa mssras ara 18 389609 S72 108 2 1424 11382 590956 7 00639 avs 151 3 193 urossz3 sea 5 2357 173 2 195 3 al 2125 usisors 5 64054 S5 76513 2063 223 5 8l 352 3 0 440191 T a348 3 58722 353 7 a7 4 6 el 324 2 0 88684 6 48989 5 84603 318 1 332 8 0 685313 8 50687 32 3173 383 1 851 2 Notices Copyright 2014 Schumann Analytics All rights rese
10. acros gt Concentration Handler gt either Initialize Concentration Handler if you want to create a new concentration curve or Show Concentration Hander if you want to add a point or see the last concentration curve you have taken Here you see your added points Type the concentration of the sample you detected Choose the peak number from the report or enter the peak area directly First enter concentration and peak area then add to ConcentrationHandler Concentration ubstanzname Enter a Mame Concentration NaN PeakMr 1 gt or Peak rea Maf Add ja Graph Zalculate unknown Concentration Area O Calculated Concentration D Max Enter the Name of the sample Do a linear fit If you want to see the fitting results first mark the Area vs Concentration display Enter an area from a sample with unknown concentration after you did the linear fit The concentration belonging to the sample will be automatically calculated graph By pressing Save Curve the curve displayed on top will automatically A popup will appear choose the concentration curve you need The choosen curve will appear in be saved with Substanzname as title the graph Fig 17 Concentration Handler 18 4 PCA Principal component analysis There is the possibility to arrange a PCA Therefore choose Start PCA in the submenu of Macros A new window will o
11. dwndwecwanecieel aqaetceniandesenauedodieranesetant 12 3 2 Creating and Using the Reference ssssssssssssessssseeeme me nee nnns 13 eiae e RE ONCA NOn E E EEE CT 13 Loading a Reference file cccecceeccsecceecceecccueceueceueceueccueceueceseecseeeueeeaeseueeeaeseeeseaeenass 14 3 3 Integrate Single Peak ccccecccceeccececeeeeseeeseeeeseeeeseeeseueeseeeeseeeseueeseessueeseeeeseeeseeeseeesaes 15 3 4 Auto Identify and Measure Peaks cccccccseccceeeeseeeeeeeeceeeeaeeseueesaeeseeesaeeeseeeseueeseeeenes 16 3 0 Concentration miae NETT TT TT TT OTT 18 4 Principal component analysis PCAY 1 Leeeeee LLL eL ieeeeee eene sena nana nana 19 Schumann Analytics Grimsehlstrasse 62 37574 Einbeck Deutschland Tel 49 0 5561 31328 47 Fax 49 0 5561 31328 51 Email info schumann analytics de 3 Web schumann analytics de 1 Information about FAIMS Data Analyser The software FAIMS Data Analyser is an optional data analysis tool created with IGOR PRO from WaveMetrics Inc Portland OR 97223 US To use the FAIMS Data Analyzer with all its functions it is necessary to install the program IGOR You can download the fully functional IGOR Pro 6 3 Demo and try it for 30 days after installation After 30 days the demo will lapse into limited functionality mode The limitations of this mode are e Saving is disabled e The clipboard contents cannot be transferred to other programs
12. er peak start Peak Start End Threshold smooth data for search min 1 end threshold 5 7 7 7 split shoulder peaks Yes w Choose Yes if a shoulder of a peak shoud be seen as as own peak Press Continue if you have finished entering the settings You were asked if you want to construct a baseline from the BaseKnots In the background you see is updated Analyse panel 16 Threshold the amplitude of the peaks have to be Correct above to be detected detected peaks LN m BEA False detected peak the amplitude is above the threshold but only the begin of the peak is below the Start End Threshold The end of the peak is automatically set at the end of the graph tempColPos VW PkThreshald Wo PkStartEndThresh X V Baseknots OQ WV PeakkKnots Intensity a u Start End Threshold the begin and Not detected peak the Not detected peak the begin and end of the peak have to be below else amplitude is under the the end is above Start End Threshold the peak is not or false detected threshold If you choose Yes for Construct Baseline from W_Baseknots a line through the Baseknots cross at start and end of a peak is drawn In the next panel you choose either the area should be detected in relation to the baseline or to y 0 and you choose the measure period After pressing continue an automatically created report appears If
13. h activated 3 D graphic You can rotate the global 3D surface as required by pressing and holding down the left mouse button and moving the mouse in the desired direction Integration The Integration button starts the fast integration for one peak Just follow the instructions in the Text Information field For the 3D integration you have two more options if your peak you want to integrate is an ellipsoid mark the Ellipse Signal checkbox tf you want to see the image in a new window while integration not advised mark New Window checkbox Save Ref This button allows you to save the current Int vs Time graph It is useful to create a library with substances You can load the saved file into the Overlay Panel to compare to your measurements A new window is opened this function is detailed described in the chapter Creating a Reference Write in Report This button located in the Text Information field writes the integration data into a table If the checkbox Automatically is marked you don t have to press the button There two different tables for each integration button one Show Report This button shows the table with your integration data The table according to the popup menu next to the button is shown Local Max Min oame as Global Max Min but only for the area you have marked before Overlay buttons The Overlay buttons temporarily store the current displayed image CV vs Int o
14. how Clear Analyse Integration Over Show Clear Fie Name F3 mit_1 o olw anne wn el o Graphic Name Intensity vs Time Time B60 484 s CV 0 341599 v Intensity 0 630039 a u Intensity a u Write in Report v Automatically Show Report 0 Compensation voltage v Fig 8 Table of the raw data Hi 3 Data Analysis 3 1 Overlaying chromatograms To be able to compare different measuring chromatograms with each other it is possible to overlay them as shown in figure 9 Select the first chromatogram to compare and press the Overlay button left under the chromatogram window Select the next chromatogram you want to compare and press again the Overlay button Press the Show button and enable the names of the curves in the text box under the graph window A click on the Annotation Button will display the legend of the curves with the selected compensation voltages ki Schumann Analytics FAIMS Data Analyzer 2 030514 Igor Pro 6 324 File Edit Data Analysis Macros Windows Graph Misc Help Gizmo Zoom Ld pmEMNTUENTTU MM mm Ov IvT F3 mit 1 0 27V p vs F3 mit 1 Zeit3 Reference Info x F3 mit 1 0 27V p F3 mit 1 0 51V p mjm oltage V 0 506328 2 3trix s Col pss Jil 1 cce ARR T 0 200 400 600 800 1000 1200 Time s Overlay Positiv xj Load Reference Remove Ref Add Ref Tag Set4 SetA
15. is not installed download and install Wavemetric s Igor Pro http www wavemetrics com support demos htm As described in the section Information about FAIMS Data Analyzer the demo version of Igor is completely sufficient 2 1 Starting FAIMS Data Analyser Double click the FAIMS Data Analyser pxp file Igor is going to start If you will asked for a serial number and the user name please fill in the information or click on limited function demo x Schumann Analytics FAIMS Data Analyzer 2 030514 Igor Pro 6 324 Edit Data Analysis Macros Windows Misc Help Gizmo Zoom Schumann Analytics FAIMS Data Analyzer 2 030514 Fig 2 Starting the FAIMS Data Analyser Start your analysis by choosing Macros gt Analyse Start Notice Starting the FAIMS Data Analyser for the first time choose Clear All and Use Default Options before starting the analysis File Edit Data Analysis Windows Misc Help Gizmo Zoom Analyse Start Clear All and Use Default Options Concentration Handler Integrate Single Peak Initialize Most Everything Baseline Fit at Regions Identify And Measure Peaks in Steps Auto Identify And Measure Peaks Table of Peak Measurements Graph of Peak Measurements Zoom To Peak Ctrl 8 Manual Areas Hide Area Tags Show Only Data 4nd Base Ctrl 0 Color Waves Fig 3 Clear all Use default options when starting the first time 2 2 Loading Data After launchi
16. n Voltage 0 45850 gt C New Window vey Show Clear Matris s Col 108 1500 2000 gt lt gt 0 0 0 2 doa Hm na Intensity vs Time M Display negative n Ch romatog ram 0 500 1000 m 1500 2000 Intensity vs File Name GoriEsche 50 1 00 Compensation 3 Voltage Display negativ Text jn Information i field T Wie in Repo Y Automatically li i Eapana M Fig 4 Overview of the FAIMS Data Analyser Main Displays positive and negative mode 2 4 Display The main displays in Fig 4 positive and negative lon currents show your data as an image The button axis is the time axis the left axis is the compensation voltage axis and the ion current is illustrated by intensity similar to the Lonestar Review Additional there are two black lines The horizontal one belongs to the Time vs Int slider The vertical line belongs to the CV vs Int slider Both indicate the position which is displayed in the Time vs Int display or Compensation Voltage vs Int display The Time vs Intensity displays middle show the current chosen intensity by Time vs Int slider The Compensation Voltage vs Intensity displays bottom right show the current chosen intensity by CV vs Int slider 2 5 Modify Displays and Graphs To modify a display right click on it A popup shows your options The uad nase ine emove rrom tarapn core options are
17. n area and right click in it Axis Reset This button scales the axis to default after expanding the image You can also set the scale to default by right click on the image graph and choose Autoscale Axes Copy Cont This button opens a new window with the current shown image and the selected intensity so you can copy it and past to other programs Ctrl C Global Max Min The Global Max Min button searches the whole image for the maximum value positive ion current or the minimum value negative ion current Both sliders are set to it and information about this point is displayed in the Text Information field 3D Graphic The 3D Graphic button displays the current displayed image as a 3D surface in a new display File Edit Data Analysis Macros Windows Gizmo Misc Help Gizmo Zoom 7 E FAIMS Data Analyzer Pos Neg Load Da ace Expand Axis Reset Time s m Matirs s Row 0 m CompensationVottage V n PS m h O tion Vol 0 329374 Copy Cont LocalMax 3D Graphic j na Segen al Matrix s Col isl D Global 3D Surface B nj xj e M 3 5 mod c a E e 100 D Overlay Show Clear Analyse integration File Name Raeuchersp40 _1 Time s Graphic Name Intensity vs Time CV Time 305 589 s CV 5 48534 v Intensity 0 0154342 a u Intensity a u Write in Report Automatically Show Report Fig 6 Overview Display wit
18. ng FAIMS Data Analyser please load your data into the program Select the folder in which your txt file is saved Mark this and click the Load Data button Now the program automatically opens the analysis panel and loads your measurements in the screens shown below 2 3 Overview E FAIMS Data Analyzer elk 3 The positiv ion current is shown CV vs Int Slider positiv 4 4 gt Matirz s Row 227 as an image N Intensity Slider positiv Time vs Int Slider positiv o e CompensationVoltage V N L gt 3 0 500 1000 1500 2000 Time s 2 C Ellipse Signal Compensation Voltage V 0 435725 gt 0 6 04 Time vs Intensity in Display positive ET Chromatogram D 500 1000 1500 2000 Time s 2200 LI File Name Gori Esche 50z 1 D 6 Com pensation Text 3 Voltage vs Information a Intensity Display field HE positiv x 0 0 iei V Automatically Show Report 2D v i i 2 i Write in Report V Automatically Show Report 2D I Compensation voltage V The negativ ion current is shown as an image Matirs s Row 145 Save to CV vs Int Slider negativ 4 0 L3 1 CompensationVottage V Time vs Int Slider negativ 2 3 Intensity Slider negativ 500 1000 Time s 0 Ellipse Signal Compensatio
19. ntegration File Name F3 1_50 0 Compensation voltage v Ready S A Fig 18 Example for Principal Component Analysis PCA 19
20. pen where you can choose with which matrix positive or negative you want to start the PCA Detected Components NaN Plot PC components to use a Then press the Run PCA button The process may take several minutes Afterwards the number of detected components are shown in the display next to the button In the display below you can now enter the number of components you want to displayed by Plot PC or Varimax The PCA operation and procedures follow the text by E R Malinowski Factor Analysis in Chemistry John Wiley and Sons 1991 if Schumann Analytics FAIMS Data Analyzer 2 030514 Igor Pro 6 324 e xi File Edit Data Analysis Macros Windows Panel Misc Help Gizmo Zoom IZiTable0 w Figen W IE W IND W RSD W P3SL P Tw Positiv lon Current Run PCA Detected Components 301 3 53293e 08 0 052588 93 7951 5497 92 1 51564e 08 0 0285791 98 7072 Pope tt components touse B 2 849 497 1 04013e 08 00183711 99 4662 m arimax 451042 5 15855e 09 000909771 99 8592 93 5336 3 10596e 09 000546937 99 9528 27 8276 2 15029e 09 0 00378081 99 9774 11 1021 1 61256e 09 000283104 99 9874 4 00909 1 36762e 09 000239741 99 9909 219617 121348e 09 0 00212399 99 9929 Cory Coni Global Mas Local Mar 3D Graptie Integration Ellipse Sio D New Wine Oyo mM amp wl Ny oO Time e 2 m gt a c a e MN A a eo 500 D Save Rel Overlay Show Clear Analyse I
21. r Time vs Int depending on the location of the button It will not be deleted by pressing Load Data but they are all deleted by choose Macros Clear All and Use Default Options Show buttons The Show buttons display the stored data by Overlay in a new panel but each one only shows the data for the display they are next to Clear buttons Whit the Clear buttons you delete the temporarily stored data by Overlay Only the data they are next to Load Data This button opens the load panel where you choose the next txt file you want to load Analysis button The Analysis buttons opens the current selected Time vs Int in a new window and initiate everything for data analysis 10 Raw Data To edit the raw data of a chromatogram or a individual spectra place the cursor near the curve and press the right mouse button a submenu is displayed in which you can choose the point edit tempColPos or tempColNeg for the data in the negative mode Fig 7 and Fig 8 H Schumann Analytics FAIMS Data Analyzer 2 030514 Igor Pro 6 32A il n 3 Fie Edit Data Analysis Macros Windows Panel Misc Help Gizmo Zoom FAIMS Data Analyzer Pl x aE Load Daal ae Expand Axis Reset Time 0 Matirs s Row fo Ja N CompensationVoltage V o N 100C Time s Ellipse Signal Compensation Voltage v 0 341599 Copy Cont Global M Local M 3D Graphic
22. rved This user manual is copyright protected and no part of this publication may be reproduced transmitted transcribed stored in a retrieval system or translated into any language or computer language in any form or any means without the prior written permission of Schumann Analytics Content Page AO AAG T E E E E esos ances ora E E vein exion nasa ap aie rer E E mene 3 1 Information about FAIMS Data Analyser cessere nnne 4 1 1 Preparing your data for analyse esseeesseeesee eene nennen 4 Export data from Review Process File Vsn esssssssssssssse menn 4 2 How to use FAIMS Data Analyser 11 e eee ieee ee ieeee nenne nennen nnn anna nana nnns 5 2 1 Starting the FAIMS Data Analyser ssssssssssssesssssse meme nennen nns 5 Be KoelneuB ice P E 6 2RCSQII HH ooC M 6 i 2 8 16 2 M T 2 5 Modify Displays and Grapns ccccccccseseccceeeeseceeceeeeeeeeceeeeceeseeeeeeeeeeeaeeeeseaaeeeesseeeessaaeeees 7 wl m 8 sop e M N 8 3 Data ANAIS iS M 12 3 1 Qverlaying chromalogr alis sedeo setedgututUtd setei
23. s in the middle of the display Set this cursor to the start point of your baseline Click the appeared Add cursor button to display the next cursor in the middle of the display You can work with up to eight cursors They were connected alphabetical after pressing the Construct Baseline button 15 After pressing Integrate the area is calculated and shown next to the button Now note the area by Concentration Handler or use the Add Tag button Auto Identify and Measure Peaks To start the automatic peak analyse choose Macros gt Auto Identify And Measure Peaks A new panel is shown There you declare the parameters for peak integration s FAIMS Data Analyzer 1 0 2 Igor Pro 6 32 Sete Windows Panel Misc Help i Load Data Concentration b Initialize Most Everything Baseline Fit at Regions Identify And Measure Peaks in Steps gt a Auto Identify And Measure Peaks 1 Table of Peak Measurements Choose positive unipolar if you are If you have loaded external analysing the Time vs Int pos else baseline data choose negative unipolar for analysing Time here vs Int neg The data you want to integrate is automatically choosen InitldentifyPeaks AA peak data wave incly ing baseline coordinates for 9 data wave Choose an Ze y integration type kaseline peak polarity positive Unipolar Peak Threshold or 0 For auto thresholds default integration type Ent
24. the cursor is marked you can change the size and position of the objects If the A is marked you can change the title by clicking on Your Report Title Here You can delete an object by pressing delete while it is flagged The graph as seen before Peak Report Layout ie 6 peaks trapezoidal area Your Report Title Here tempColPos MW PkThreshold W PkStartEndThresh X WV BaseKnots m grum W Peakknots MW BaselineFit A table with number of the peak time of the peak center peak amplitude Intensity a u peak area start and end time of the peak S w_PRSenters W PkAmps W PkFWHMs W_PKX1 W PKX2 2 1424 anen 575990 10312 13755 asal 3 nas tas S44559 T46731 2063 2223 4 aaa namaa amp 4605 aeeoe 31945 3328 s 3e3 1 03904 845620 arios 3031 8512 DN al Sms cues nose E eal on 1 lt 100 w PeaksGraph Left 0 99 Top 1 45 Width 6 27 Height 3 06 gt Concentration Handler s FAIMS Data Analyzer 1 0 2 Igor Pro 6 32A Ems Windows Layout Misc Help Load Data ccu Show Concentration Handler amp yc ok nitialize Concentration Handler The Concentration Handler is an easy way to take a RII p concentration curve Open the Concentration Handler by pressing M
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