Declaration of Conformity - GE Healthcare Life Sciences
Contents
1. transformed concentration values are required whether the thresholds are required if used and whether the graphic of the standard curve is required together with statistical calculations Press enter to move to the next method definition box Saving Method Definition The complete method has now been defined It can be saved over the top of an existing method F3 or more usually as anew method Use Y to locate an empty method slot and F4 to append Then enter the method name using the alphanumeric function and press enter This new method is then placed as 1 in the start up box that now appears Issue 1 1 02 2003 Biotrak II plate reader user manual English Running a method This can either be done immediately after defining a method press 1 or by recalling an existing method press 2 select using Y and F4 Each plate that is run for each method has to have an unique ID This should be entered all data is stored in the instrument memory 1 Mb and can be recalled for analysis at a later date using this ID and the Recall Plate option on the main menu To start the measurement press either key 1 RUN or 2 Select a method The plate carrier will move out now allowing to load the prepared microplate The F3 key allows to select that either the results is printed automatically after the measurement F3 Auto or that the data are stored in the memory only for later printing F3 Rec This can speed up the measurement2. Instrument A pop up window will appear which shows the download progress After the download is finished the methods are shown in the left pane When you want to store all these methods click on the Select all button then click on the button to copy the methods into the right pane Afterwards click on the Write to File button to save the methods into a desired file In case one or more methods should not be saved they can be selected by a mouse click and deleted Adding methods In order to add methods into the reader memory the existing methods must be downloaded and copied into the right pane first Then the methods in the left pane should be deleted select all and Delete Now the new methods can be loaded either from a file are from the simulation The desired files are copied now into the left pane As last step click on the Upload to Instrument button to start the upload process Note Uploading methods will override all methods currently stored in the reader memory Issue 1 1 02 2003 Biotrak II plate reader user manual English 11 Plate upload and download For plate up and download choose Arrange plates from the file menu Arrange plates Sources Selection only Download Upload Read file write file Si 4 13 02 05 22 12 256 5 g 13 02 03 2211 225 4 S 44 13 02 05 22 09 2567 5 OF 13 02 03 21 05 2224 S zad 13 02 03 16 55 testnew 5 94 2r 01 03 23 27 222 5 5 00
3. and methods are stored in different files on this Method download upload saving and sorting Connect the reader and the computer with the serial cable supplied Locate the folder Reader gt Upload Download on the CD and double click on the file vrsim exe to load the simulation software Yisual Reader Simulation 3 2 Files Special Arrange methods Arrange plates Seral communication Exit Select the correct COM port serial port in the File menu Serial communication Then select Arrange methods A new window like the one below will appear 10 Biotrak II plate reader user manual English Issue 1 1 02 2003 x Method Definition Sources Method Definition Targets Upload to Instrument Read from File winte to Bile Include Simulation Update Simulation gt Select Al Unselect Delete Select Al Unselect Delete Close Descriptions of keys Download from Instrument Clicking on this button will download the methods stored in the reader Read from File Load methods stored on the computer Include Simulation Load methods that are active in the simulation abet te Usui Uploads the methods shown in the right pane to the reader write to File Stores the methods shown in the right pane into a file Update Simulation Methods shown in the right pane will be active in the simulation Method download and storage Click on the button Download from
4. of a batch of plates significantly When less than the maximum number of samples should be processed the number of existing samples can be entered This will avoid that empty well positions show unwanted results When the plate ID has been entered press the RUN or the ENTER key to start the measurement During reading the screen shows a status message A soft key labelled STOP is the only key active during reading and subsequent printing Up to 100 plate data results can be stored in memory after this is full the new results overwrite the first results To avoid this results can be downloaded to PC for archiving Recalling a plate Previously run assays are stored using the unique plate ID and can be recalled from the home page by pressing key 3 and the ID Uploading and downloading plate data results and assay protocols The ability to download plate data results means that such results can be permanently archived in addition to having a print out or download to spreadsheet New assay protocols can be uploaded into the instrument easily meaning that they can be prepared away from the laboratory Note also that when the instrument s storage capacity of 120 methods and 100 plate results is full these can be downloaded for archiving and removed from the instrument to make space for new ones The procedures for doing this are easily achieved using the instrument simulation software on the CD supplied with the instrument since late data
5. screen Method Definition Transformation Use this option to define a calculation equation to be applied to each well The equation should be written down first and is then entered in stages all typical mathematical functions are available sqr abs log log10 x etc and Pow means power of x There are three groups of elements that can be selected by pressing the F1 Alt key The first group contains controls single and average the second one operators like etc and the third one all available mathematical and logical functions Use F2 and F3 to select the desired element and F4 to place it Method Definition Transform Formula will be applied to each well s stands for original well value Uix avygl COjylayglQC j avglCOjy 1 avg lHP avg CO avg Qc Oc Press enter to move to the next method definition screen 8 Biotrak II plate reader user manual English Issue 1 1 02 2003 Method Definition Thresholds Use this option to define the threshold limits to determine whether a sample is negative positive or in the grey zone where they should be re tested Limits can be defined for the measured abs value OD transformed equation or concentration values depending on the test protocol This option enables results for screening tests to be grouped The example below shows show thresholds should be entered a negative result is any OD that is 0 8 or more above that of the positive control wherea
6. the mains power supply Disconnect the instrument from the computer 3 Carefully wipe all the outside surfaces of the instrument and the plate support area with a wad of cotton wool that has been soaked in the disinfection solution Ensure that disposable gloves are worn 4 Place the instrument in to a large plastic bag 5 Place a wad of cotton wool that has been soaked in the disinfection solution in to the plastic bag Ensure that the wad is not touching the instrument 6 Close and seal the plastic bag 7 Leave the instrument to stand in the plastic bag for at least 24 hours 8 After the standing time remove the instrument from the plastic bag and wipe all the outside surfaces of the instrument and the plate support area with a wad of cotton wool which has been soaked in a 50 Alcohol solution 9 Repeat the disinfection procedure on any accessories which are also being moved or returned Issue 1 1 02 2003 Biotrak II plate reader user manual English 17 SPECIFICATION AND WARRANTY Wavelength range 400 800 nm Filters Dielectric interference filters Filter wheel for up to six filters 405 450 492 and 620 nm supplied as standard Photometric method Multi channel optical system with self calibration and digital light control Tungsten Halogen expected life time 10 million readings Multiple silicon photodiodes 1 0 0 2 0 OD Specifications are measured at a constant ambient temperature and are typical o
7. 8S 27 01 03 23 26 222 5 25 4556 2f 01 03 21 41 222 5 S J 27 01 03 21 35 222 5 5 59 2r 01 03 21 34 222 5 5S 58 27 01 03 21 33 222 5 5 4567 27 01 03 21 25 222 5 5S gapga 27 01 03 20 10 222 5 5 888 27 01 03 20 04 222 5 5 44 27 01 03 19 55 222 5 Select All Unselect Delete Close The plate window is showing the following information from left to the right Sequential number of plates the newest plate has the number 001 Code for the data source S simulation R reader Fx file Plate ID Data and time of data generation Method name Storing plates on a file Connect the reader and computer with the serial cable supplied Press the download key the measured data are transferred from the reader to the PC Select those data who should be saved and click on the Write file key The screen prompts for a file name after entering the desired filename click on the save key 12 Biotrak II plate reader user manual English Issue 1 1 02 2003 Messages Most messages are self explanatory and relate to use of the instrument Set up Printer Use this option to define which printer is connected to the plate reader literally dozens of printer drivers are available on the instrument Press 1 to reveal the generic types and use Y with to select between Compatible Epson HP Other Use Y to highlight the printer required and press enter so that the options of paper size A4 or letter using F1 colou
8. NTS Declaration of Conformity 1 UNPACKING POSITIONING AND INSTALLATION 3 Power Requirements 3 Environmental Requirements 3 Essential Safety Notes 4 OPERATION 5 Introduction 5 Keypad and display 6 Method Definition 7 Method Definition Filters 7 Method Definition Layout for controls and standards 7 Method Definition Samples amp Method Definition Calculations amp Method Definition Factors amp Method Definition Transformation 8 Method Definition Thresholds 9 Method Definition Validation 9 Method Definition Calibration 9 Method Definition Printout 9 Saving Method Definition 9 Running a method 10 Recalling a plate 10 Uploading and downloading plate data results and assay protocols 10 Method download upload saving and sorting 10 Descriptions of keys id Method download and storage ll Adding methods 11l Plate upload and download I Storing plates on a file I Messages 13 Set up 13 Printer 13 Filters 13 Date Time 13 Contrast 13 Language 13 ACCESSORIES 14 MAINTENANCE 15 After Sales Support 15 Fuse Replacement 15 Filter Replacement 16 Cleaning an interference filter 16 External cleaning 16 Instrument disinfection 17 SPECIFICATION AND WARRANTY 18 2 Biotrak II plate reader user manual English Issue 1 1 02 2003 Unpacking Positioning and Installation E Visually inspect the container for damage before opening it Report any damage immediately to the forwarding agent or to the delivery carrier 2A Place
9. Ue lochrom Biochrom Ltd Certificate No Declaration of Conformity 890333 This is to certify that the Biotrak II Microplate Reader Part number 80 2115 80 81 82 83 96 well version Serial number 88000 onwards manufactured for Biochrom Ltd by its subsidiary Asys Hitech GmbH Nordstrasse 4 A 5301 Eugendorf Austria conforms to the requirements of the following Directives 73 23 EEC amp 89 336 EEC Standards to which conformity is declared EN 61 010 1 1993 and A2 1995 Safety requirements for electrical equipment for measurement control and laboratory use EN 50 081 1 1992 Electromagnetic compatibility Generic emission standard part 1 Residential commercial and light industrial environments EN 50 082 1 1997 Electromagnetic compatibility Generic immunity standard part Residential commercial and light industrial environments EN 1658 1996 In Vitro Diagnostics Requirements for marking Signed ah David Parr Managing Director Biochrom Ltd Postal address Biochrom Ltd 22 Cambridge Science Park Milton Road Cambridge CB4 OFJ England Registered in England No 974213 Dated 23 November 2002 Telephone Telefax 44 1223 423723 44 1223 420164 e mail enquiries biochrom co uk website http www biochrom co uk Registered Office 22 Cambridge Science Park Milton Road Cambridge CB4 4FJ England Issue 1 1 02 2003 Biotrak II plate reader user manual English CONTE
10. a filter wheel that contains up to 6 changeable filters To replace one or more of these filters please perform the following procedure l Switch off the instrument 2x Remove the holding screw of the filter compartment cover which you can find at the right side of the instrument Remove the filter compartment cover 3 Rotate the filter wheel until the required filter or filter position is at the front The filter position one is marked with a yellow sticker Filter position two is located above the filter one 4 Pull the filter that is to be changed out of the filter wheel 5 Carefully insert the new filter in to the filter wheel 6 Close the filter compartment cover 7 Switch the instrument on 8 After changing a filter a calibration run where the lamp intensity is for each wavelength is adjusted to a value that guarantees optimum measurement conditions and the highest resolution is performed Cleaning an interference filter If an interference filter is dirty it needs to cleaned For the cleaning procedure you need low lint cotton tipped swabs and a lens cleaning solution or reagent grade isopropyl alcohol First blow off dust and dirt with pressurised gas Apply a few drops of the cleaner on both sides of the filter and wipe off the dirt with the cotton swabs Allow the cleaner to evaporate and then visually check the filter surface for streaks or spots If there are any repeat the procedure When streaks or spots are v
11. e absorbance of the blank value s are automatically subtracted from the absorbance of the other wells NC Negative Control Use for qualitative screening tests PC Positive Control Use for qualitative screening tests LP Low Positive Use to see if results are outside pre defined thresholds HP High Positive Use to see if results are outside pre defined thresholds CO Control Use as required QC Quality Control Use as required ST Standards Use for quantitative determinations a maximum of 16 standards can be used e Press F4 to place the required well type in the plate layout that is shown on the display Enter replicates by repeat pressing of F4 as appropriate By alternating between F1 and F4 it is possible to build up the plate as required You can come back to edit the layout if required Use 4 to move around the plate layout if necessary as you do this you can see what has been assigned to each well e When ST is selected using F1 ST 01 option appears as F2 After placing these on the layout press F2 to get ST 02 A total of 16 standards with replicates can be built up in this way e When doing standards note that the concentrations are entered later on Method Definition Layout Locations of Blk Dd2 Std 03 A01 402 TItTL RRR ee Type QAFE nc LP HP co oc Std gt Options Place Press enter to move to the next method definition box Issue 1 1 02 2003 Biotrak II plate reader user manual English Me
12. f a production unit As part of our policy of continuous development we reserve the right to alter specifications without notice Warranty Your supplier guarantees that the product supplied has been thoroughly tested to ensure that it meets its published specification The warranty included in the conditions of supply 1s valid for 12 months only if the product has been used according to the instructions supplied They can accept no liability for loss or damage however caused arising from the faulty or incorrect use of this product 18 Biotrak II plate reader user manual English Issue 1 1 02 2003
13. ided Operating Temperature 10 to 35 C Operating Humidity 0 to 80 Non condensing Environmental conditions Humidity 5 to 95 non condensing storage only Maximum altitude 2000m Before the instrument is installed and switched on it should be left to stand for at least 2 hours so there is no possibility of condensation causing a damage or malfunction Please Note e Only use the power cable supplied with the instrument or a power cable with protective earth connection carrying the CE mark e The power outlet the instrument is connected to has to meet the applicable technical safety requirements e When the instrument is switched on part of the instrument initialization process involves the microplate tray coming out and going back in again Issue 1 1 02 2003 Biotrak II plate reader user manual English Essential Safety Notes e If inflammable toxic or biologically hazardous substances are used when operating the equipment please observe the instructions and precautions enclosed with such substance e Never spill fluids in or on the equipment e Wash your hands thoroughly after handling test fluids e If equipment has been in contact with hazardous substances it must be disinfected prior to shipment in accordance with the effective provisions e Do not touch the plate during movement of the plate transport risk of injury e This instrument should be serviced by an authorized service person only since it contain
14. isible which cannot be removed by the procedure as described above most probably they are inside the filter In such a case the filter must be replaced Note Interference filters do have a limited lifetime of a few years depending on the humidity and ambient temperature Under tropical conditions a filter may get unusable within less than two years External cleaning This instrument is a precision instrument and requires regular cleaning to ensure the continued precision Liquid Spills If any liquid is spilled in the instrument it should be IMMEDIATELY removed so that the liquid does not run in to the Optical System and causes a loss of accuracy Regular cleaning The housing of the instrument should be cleaned regularly with a mild household cleaning agent Warning Do not use aggressive solutions 16 Biotrak II plate reader user manual English Issue 1 1 02 2003 Instrument disinfection All parts of the instrument that come into contact with patient sera or positive controls must be treated as potentially infectious It is therefore very important that the instrument is thoroughly disinfected before it is removed from the laboratory or any servicing is performed on it Disinfection Procedure If the laboratory has no specific disinfection procedure the following procedure should be used to disinfect the instrument The instrument should be disinfected using a suitable disinfection solution l Disconnect the instrument from
15. ll benefit of the instrument results have to be downloaded either to printer or to spreadsheet the instrument is delivered with a serial and parallel cable for connection to PC and printer respectively The optical system consists of a visible lamp condenser lens interference filters on a six position filter wheel an optical fibre bundle to take light to a focusing lens for each of the eight measurement channels and eight silicon photo detectors with another lens in front of each of these The instrument features digital light control The lamp intensity is monitored and controlled so that measurement errors caused by undetected light control problems are prevented Thus every time a filter and wavelength is changed a calibration run is initiated and the lamp intensity for each wavelength is adjusted to a value that guarantees optimum measurement conditions and the highest resolution When the first of the calibration runs is done the light intensity for each wavelength is recorded by the system so that for any subsequent measurements the light intensity can be set to the stored value and a check is only necessary This output for each channel is known as the 100 value or zero measurement and several readings are taken in the centre of each well When samples are measured absorbance is calculated relative to the 100 values The resulting signal is then filtered and displayed The advantages of digital light control are e Prevention of drift
16. of a specific PIN oe Text entry when required or appropriate is also easy and described when its use first appears The main menu screen is displayed on start up This and all the other displays are divided into three main sections Biotrak II Reader o the top line which shows the current topic lt SELECT Method RECALL Plate b DEFINE Method SETUP Statt measurement using current method o the bottom line which indicates the description of the soft keys e the main section which serves to present the entry parameters In some cases especially when a graphical content is shown the top and or the bottom line are not shown Run This option starts a measurement with the last used or defined method This option selects a pre defined or previously stored method to use This option recalls previously stored measurement data beginning or by modification of a previously stored or default method up calibration to be set up 6 Biotrak II plate reader user manual English Issue 1 1 02 2003 Method Definition Method Definition Filters e Measurement Use the 4 gt keys to select the filter to be used for measurement e Reference Use the 4 keys to select the filter to be used as a reference use none for no filter The reference value usually 620nm is subtracted from the measured value to correct for imperfections etc on the plastic microplate these can have a great effect on low OD measuremen
17. ort We supply support agreements that help you to fulfil the demands of regulatory guidelines concerning GLP GMT Calibration certification using filters traceable to international standards e Certificated engineers and calibrated test equipment e Approved to ISO 9001 standard Choice of agreement apart from break down coverage can include e Preventative maintenance Certification Observe all necessary precautions if dealing with hazardous samples or solvents User maintenance is restricted to changing the filters and mains fuses For any other maintenance operation or rectification contact your local supplier Fuse Replacement The following steps must be performed to replace the fuse which is located above the power cable connection in the rear panel of the instrument l Switch off the instrument and unplug the power cord Open the plastic cover of the fuse compartment by inserting a screw driver into the slot at the left side of the cover and pushing the cover out 3 Pull the fuse holder away from the instrument 4 Replace the fuses only with the same type and rating Ensure that the fuses have the correct rating T 3 5 Amp slow blow type 5 Replace the fuse holder 6 Reconnect the power cord and switch the instrument on WARNING If the fuse continues to blow please call for service Issue 1 1 02 2003 Biotrak II plate reader user manual English 15 Filter Replacement The instrument 1s supplied with
18. out of the optimum operating range a problem with analogue light control systems e Detection of lamp failure e Active filter detection if the light value is different to the value stored in memory due to a wrongly inserted or defective filter an error message will be displayed In addition to the four filters supplied as standard a wide range of other filters 1s available to enhance the capability of the product User knowledge of ELISA techniques and microplate handling is assumed Issue 1 1 02 2003 Biotrak II plate reader user manual English Keypad and display The 240 x 128 pixel high resolution graphical liquid crystal display provides the user with set up parameters and experimental results and the keypad is a hard wearing spill proof membrane Navigating around the menu structure is simple 1 press the corresponding number on the keypad to enter the user mode choices or numeric data 2 press the soft key on the keypad directly below the corresponding option on the display F1 F2 F3 and F4 to select that option use the four 4 Y cursor keys to navigate around the options when the prompts appear use the green enter key to move forward to the next display page use the red stop key as an escape mechanism to go back incorrect entries can be removed using the lt key the keypad can be locked when not in use using the lock key default PIN is 9999 Service engineers can access service functions from here by entry
19. r or black white F2 serial or parallel output F3 can be selected Press F4 to complete printer selection NOTE If using a Canon printer use the Epson FX printer drivers Download to Spreadsheet Results can be downloaded directly to Excel when the PC has the Spreadsheet Interface Software installed and the two are linked with the serial cable supplied with the instrument detailed instructions are supplied with the software Using the spreadsheet results can be formatted or manipulated as appropriate prior to inclusion in reports or archiving saving to hard disk Filters Filters can be easily changed or removed for cleaning see Maintenance When this is done they must be calibrated using this option by pressing the F3 or F4 key Date Time Use this option to change the date and time with Y and the keypad A choice of am pm or 24 hour clock is available Contrast Use this option to change the contrast of the liquid crystal display using the F1 and F2 keys Language Use Y to select the language that is used on the displays and print outs Issue 1 1 02 2003 Biotrak II plate reader user manual English 13 ACCESSORIES Part Number 80 2112 79 Hewlett Packard DeskJet printer Biotrak II washer 80 2115 90 405 nm filter 80 2115 91 450 nm filter 80 2115 92 492 nm filter 80 2115 98 80 2115 93 562 nm filter 14 Biotrak II plate reader user manual English Issue 1 1 02 2003 MAINTENANCE After Sales Supp
20. s a positive result is 0 6 or below that of the positive control Anything in between these is in the grey zone and should be re tested Method Definition Thresholds Data source avgtPCi 0 6 avgtPCi 0 6 Press enter to move to the next method definition box Method Definition Validation Use this option to apply parameters to ensure that the test 1s valid within the requirements stated by the assay test protocol If the parameters go outside of the verification limits defined by the test the results are considered not valid Press enter to move to the next method definition box Method Definition Calibration Use this option to enter the values of the standards if they have been selected in the plate layout you can go back and edit the plate if required using the stop key Use Y to move onto the next Use F1 as a quick way of entering a serial dilution series Units can be defined using the alphanumeric routine Select the analysis model for the standard curve using 4 Linear regression Parabolic Cubic Point to Point linear interpolation Spline 4 parameter Select the y and x axis format required for presentation of the results linear linear log linear linear log log log Press enter to move to the next method definition box Method Definition Printout Use this option to define what is to be printed for the results Use 4 lt Y to select if the plate layout is required whether the absorbance
21. s live parts There are a number of warning labels and symbols on your instrument These are there to inform you where potential danger exists or particular caution is required Before commencing installation please take time to familiarise yourself with these symbols and their meaning Caution refer to accompanying documents Background colour is yellow symbol and outline are black 4 Biotrak II plate reader user manual English Issue 1 1 02 2003 OPERATION Introduction Your visible microplate reader is intended for in Vitro Diagnostic use The software supplied with the reader sets a new benchmark for flexibility powerful evaluation options and ease of use Your instrument e can save up to 120 methods e note that this includes approx 80 pre programmed Biotrak assay protocols which can be deleted if required to free up additional method storage capability for other assay protocols e goto www amershambiosciences com for information on the range of cell biology assays available measures a wide range of colorimetric end point assays performs kinetic measurements evaluates the results with on board software outputs results to a range of printers uploads new assay protocols from PC if required can download results directly to spreadsheet via the serial interface for manipulation in Excel and for data storage and archiving e can save up to 100 sets of plate results these can be downloaded to PC for archiving if required To get the fu
22. the carton in an upright position and open it 3 Remove the upper cardboard box carefully 4 Lift the instrument out of the carton and place it on a flat surface free from dust vibration and away from direct sunlight 3 Visually inspect the instrument for loose bent or broken parts Report any damage immediately 6 Compare the instrument s serial number attached on the rear panel of the instrument against the serial number of the instrument on the delivery shipping note and packing list 7 Check the instrument accessories against the delivery shipping note and packing list 8 Please save all packing materials as they may be required for later transportation Power Requirements The instrument has a universal power supply that requires no setting for the local voltage The voltage must be in the range from 85 to 264 V AC 48 to 400 Hz WARNING For safe operation of the equipment it is mandatory that it is connected to a wall socket equipped with a ground earth connector Ensure that the voltage supplied to the instrument is correct to this specification and the correct rating and type of fuses fitted Fuse Ratings T3 5A For instructions how to change fuses please refer to chapter 8 of this manual Environmental Requirements The instrument should be placed on an even surface that is free from dust solvents and corrosive vapours 50 cm x 35 cm length x width Vibration and direct sunlight should be avo
23. thod Definition Samples e Use gt lt v to move around the plate layout if necessary e Place the first sample and replicates using F4 the press F1 to get Smp 02 Use F4 to place the second sample and repeat as necessary e Press F2 to fill define all remaining wells as samples no replicates To erase all already defined sample positions press the Backspace for a few seconds Press enter to move to the next method definition box Method Definition Calculations Use the 4 gt to select if an option is required and Y to move onto the next Selected options are displayed in sequence when this method definition box is left Factors Transformation Thresholds Validation Factors and Validations are not used for Biotrak assays Press enter to move to the next method definition screen Depending on the selected options different screens will be shown Method Definition Factors Use this option to enter a constant specific to a particular test for example to compensate for batch variations if biological materials are being used as part of the assay kit Thus if the material is less biologically active than it should be the manufacturer will specify the use of a factor or factors to normalise results to what they should be Note that factors entered here are not used as factors per se in user defined equations but that the normalised results are used in such equations Press enter to move to the next method definition
24. ts e Shake Press F2 if plate shaking is required before measurement this is often used if working with cells so they do not settle on bottom of well Enter the shaking time required in seconds and the degree of shaking The options are slow medium fast extreme shake and a combination of speed and travel distance trail and error will identify the most relevant option for your application Press F4 to accept the entries e Kinetics Press F4 if kinetics is required The assay time is defined from the assay measurement interval and the number of measurements required note that a measurement is taken at time zero Press F4 to accept the entries Press enter to move to the next method definition box Method Definition Layout for controls and standards e Press F3 to define default directions horizontal or vertical for replicates and samples horizontal and vertical respectively are recommended Press F4 to accept the entries e Press F1 to select the well type required from the options listed at the bottom of the display box Note that the terms are just names and whatever you want them to be they are not tied For example LP and HP can be used to substitute for terms T1 and T2 defined in a specific test kit LP and HP do not have to be used in threshold determinations This is so that terms used previously by the user are not forgotten e Suggested descriptions and typical uses where appropriate are given below Blk Blank Th
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