Home

User Manual - ProGroup Instrument Corp.

1. e A H j Enpty last rou to e UFF Liquid handling speed e High Change tips _ Link files ra LINK TO FILE is used to indicate what the system should do after it completes the file it is currently running If you want the system to stop at the end of the file do not enter anything in the link file field If you want the system to perform another file after the present file is complete link to the desired file CHANGE TIPS is used to indicate if the tips should be removed after the first liquid is dispensed and a new set of tips from the tip magazine should be placed on the pipette head thus decreasing carryover If you don t want to change tips then the same tips will be used throughout the entire operation 41 13 42 49 Serial Di lution Actual Values Screen 3 43 Fill plate P gt D EE Gm H rus 00 eee First Tip Rou oH E mj A B Fill plate DB POLLS 19 39 22 Above screen represents WellPro 24 channel 384 well plate FILL PLATE ROWS TO DILUTE is used to indicate the rows of the plate are to be included in the serial dilution Rows are designated by letters A through P back to front for 384 well plates in the 24 across configuration and 1 through 16 in the 16 across configuration Rows are designated by letters A through H for 96 well plates in 12 across configuration and 1 through 8 in the 8 across configu
2. ae 1 TU 1 Tanster Yo lume 4 58uL sWe i Def Values Next I eb Hele 13 59 09 Plate to Plate chuil A Actual Values Screen 344 Plate 1 type FLAT Plate 7 tupe FLAT Link files 9 Rst NAT and NAT is used to reset the next available tip row to be used in the tip rack This will set the tip tracking feature and will keep track of last tip row used 59 8 HOST CONTROL CHERRY PICKING 6 1 Overview Host Control is the process for sending commands from a computer into the serial port of the WellPro This is also used for sending information from a computer such as excel spreadsheet data to the WellPro for our cherry picker single channel nozzle Refer to cherry picking manual for more information on the operation of the single channel module Hain Menu Plate Serial Plate Filling Dilution To Plate Cherry ess eere S SUPPORT Pressing the Host Control button will open up the Host Control screen Cherry Picking Program status OF Head Pos steps Deck Pos zteps Tip Unl BuL Connect the PC using the Exit ex Be Serial Portland run err I reo Picking of tudre Host Control Cherry Picking Screen allows for connection to a computer or other device via the serial port Head position Deck position and Tip Volume can be programmed from software such as hyper terminal net and others This function is used when the WellPro needs to be integrated into a larger platform whi
3. 9 A H Nix volume 53 uL Plate type FLAT Trof volume at start e rrmmL Liquid handling speed e Link file 8 PLATE ORIENTATION is used to indicate how the plate is positioned The value that is selected indicates the number of wells that will be under the pipette tips at one time Important this must be set at A H landscape at all times if you have a short bed WellPro The short bed can only fill plates in landscape but the long bed WellPro can fill in landscape and portrait orientation A H for 96 well plate landscape orientation 1 12 for 96 well plate portrait orientation A P for 384 well plate landscape orientation 1 24 for 384 portrait orientation MIX VOLUME is used to specify the volume of the aspirate delivery cycles used to mix the contents of the well after transfer PLATE TYPE is used to indicate the design of the bottom of the plate V shape U shape Flat This information is used to determine the height of the liquid in each well and thus calculate how far down 24 into the wells the tips should go If you are not using a standard microplate select custom one of the ten custom programs The custom programs for non standard plates are configured in the settings configuration from the main menu In the settings menu you can set the HEIGHT and determine the amount to raise or lower the tip height The height parameter can be set from 100 to 3500
4. 1 Aspirate sample from source plate 2 Deliver sample to receiver plate 3 Replace used tips with fresh ones and proceed to the next sample row etc 7 2 Types of Plate to Plate Transfer There are two general types of Plate to Plate Transfer 1 transfer any single row in one plate to any other single row in another plate 2 transfer any single row in one plate to two or more rows in another plate The program allows you to specify the direction of the transfer The default direction is from plate 2 to plate 1 but the system also allows you to transfer from plate 1 to plate 2 so that either plate can be used as the DESTINATION plate As examples you can transfer from row A of plate 1 to row B of plate 2 or you can transfer from row A of plate 2 to rows A B and C of plate 1 This enables you to set up an assay plate to do duplicates triplicates etc At the main menu screen you have the option of selecting a process and creating a file with your parameters and saving the file Select the Plate to Plate button for changing plate transfer parameters Hain Menu Plate Serial Plate Filling Dilution To Plate Cherry Pick ing sett ings 2 Saree S RESTI saree qe 47 7 3 Creating and Editing a Plate to Plate Transfer File Transferring an aliquot of sample from one plate to another is called plate to plate transfer or replica plating This technique allows for transfer of samples in a SOURCE plate to a RECEIVER pl
5. Air Gap This feature moves the liquid head one extra position after each dispense This feature helps dispense all liquid that remains in the tip after each dispense Factory settings should be acceptable for most applications but may need to be changed depending on viscosity of fluid A value of 20 steps for 24 channel head will result in approximately 1ul air gap A value of 8 steps for 12 channel head will result in approximately 1ul air gap The air volume should be sufficient to assist the separation of the droplet from the tip to the well bottom but not so great that air bubbles become a problem The air gap is parameter that is often optimized by trial and error Buzzer Clicking on the Disable Enable button will toggle the buzzer to disable or enable This will enable or disable sound when you touch icons buttons on the touch screen Backlight This feature will put the touch screen in a sleep mode after not using for a specified amount of minutes Sleep mode will extend the life of the touch screen Password The password comes with a factory setting of 1234 The password can be changed to your personal preference After choosing a password you should document the password and store in a safe place The password is required to access the settings area of the software Feature increases or decreases the contrast of the touch screen display Battery Tracks the battery life of the touch screen display Optimizing Pipetting Per
6. 1 2 Principle of Operation The ProGroup Instrument Corporation WellPro WP3000 Liquid Handler consists of a hand held touch screen controller and a liquid handling unit A variety of reagent containers can be used to hold liquids that will be added to the wells in a plate or have been removed from a plate 1 2 1 Touch Screen Controller The ProGroup Instrument Corporation WellPro WP3000 Liquid Handler includes a touch screen display that is used to program all operations of the unit The controller allows the user to control parameters such as the volumes to transfer mix or dispense the transfer speed and whether to change tips between transfers 1 2 2 Liquid Handling Unit The ProGroup Instrument Corporation WellPro WP3000 Liquid Handler is an electro mechanical transfer unit that is comprised of two basic parts a table and a vertical head assembly The table accommodates a series of interchangeable templates for the short base unit and a single template for the long base that position the reagent containers and a magazine with the disposable tips etc During operation the table slides back and forth to bring the appropriate component under the head assembly The short base unit is several inches shorter so that it can fit inside a hood The shorter base unit does not have the plate filling in portrait mode feature like the long base due to the table space being shorter The head assembly consists of three basic parts e aplung
7. Actual Values Screen 3 4 Plate 1 type FLAT Plate 7 tupe FLAT Link files 9 EB Help Above display for WellPro 12 channel 96 well plate Plate to Plate Transfer B uL Actual Values Screen 344 Plate 1 type FLAT Plate 2 type FLAT Link file 19 39 02 Above display for WellPro 24 channel 384 well plate ENTER FIRST TIP ROW is used to specify which row of tips to use Rows of the tip magazine are designated by letters A through P back to front 24 channel head or A through H back to front for 12 channel head SOURCE ROW is used to indicate which row of the SOURCE plate is used to withdraw samples from This parameter only appears if the 1 to SEVERAL mode is selected in this file In that instance the SOURCE row specified acts as the single source row for transfer to several receiving or DESTINATION rows First Tip Row NAT Setting the next First Tip Row to NAT will keep track of next available tip that has not been used This should be set at NAT if you are linking programs or wanting to keep track of next row of tips to be used while manual running programs If you want to reset the next available tip row to the beginning then at screen one press Rst NAT See below 22 IE Ba Plate to Plate SuULA A Actual Values Plate 1 tupe Plate 4 type Link file When you press Rst NAT located on screen one a Pop Up screen will be displayed asking to make sure the deck is OK If you press OK an
8. Enter in a 1200 value and press enter The liquid head will move down Click on the plunger Enter in a 1100 value and press enter The liquid head will move up Pick and place the trough so that the end of the pipette tip is submerged into the distilled water While the tip is submerged enter a value of 1000 in the Plunger setting and press enter This will aspirate 5ul of water Next remove the trough and press the 25 negative arrow key ONCE Now take a fine tip felt pin and carefully mark a line where the bottom of water line is located on the tip while still on the nozzle Now press 25 arrow once and then plus 25 arrow once Perform this sequence 16 times After performing this operation the water line should be at the original position and at the felt tip marked line If it is below the line then the backlash setting needs to be reduced and if water level is above the felt tip marked line then the backlash number needs to be increased Continue this process until a backlash setting is found so that the water movement distance level is repeatable 75 Tip Volume Volume pipette tip being used This will usually be 200ul for 96 well plate 12 channel head WellPro or 50ul for 284 well plate 24 channel head WellPro EJECT This setting determines how far the tip ejection bar moves to eject tips This setting should never need adjusting If tips are not ejecting from nozzle then please contact a ProGroup technician for technical ass
9. This User Manual also describes features and specifications of the WellPro Model 3000 Liquid Handler hardware and on board software This manual includes various options that may not be installed in your instrument If you desire to add these options please contact ProGroup Instrument Corporation or your local distributor for further information WellPro User Manual 01 03 07 iv TABLE OF CONTENTS SAFETY SYMBOLS AND MARKINGS coi esee is Ives desto oov ei ue a III ABOUT THE USER MANUAL Jive Saas reti VES conte AA TIR AGGHAC dadas IV SABEE OPIGONTENES 255 tai quate aee RA Lc eeu eed can D L LUE Oda V T ANTRODUCTION cui ios 1 1 1 OVER ata tido 1 12 HPRINCIPEE O OPERATION a 2 1 2 1 MOUCH Screen Controller a a e e a de ue 2 1 2 2 Liquid Handling Standard Base Unit ooooooooocoooccoroccorccrnocconrcconocrooocconoccnons 2 1 2 3 WellPro Reagent ContainerS sssesesseseecsscseesescesoesescsecsoeseecssceceseeceseecesoreeseee 4 1 2 4 WellPro Templates and configurations sssessssessesoescesseseecesoeseecescsceseecesoeseseee 5 2 ANSTALLARION PROCEDURES suas were dia eee 14 2 1 SSA O UM I T ML MC a MDC Ine Deon DE 14 2 2 LOCATION OF THE UNIT IN THE LABORATORY cccccccccccccccccccccccccccceccccccceccscecceccecescescecesces 14 2 3 POWER REQUIREMENTS ci dida 15 2 4 U NPACKING AND INSPECTION 454 cies Dno ster Peru veu E ke eto E UE E REO NEA E vo vw Rv edo better qe E CER 16 2 5 INITIAL INSTALLATION OF THE WE
10. Press Rst Volume and then Track and OK After pressing Rst Vol Track and OK the program takes you back to screen 1 of 3 When you press GO to run the program you will then be prompted to enter volume in Trof Enter Volume in Trof New Volume The software will now keep track of volume used in the trof during operation and running various programs and linking of programs LIQUID HANDLING SPEEDS is used to select the rate sample delivery from the tips rapid medium or slow LINK FILE is used to link and run a previously saved program immediately after current program If link file is left blank then system will run current program only The area immediately to the right of link file displays a number which represents a time delay between the link files if desired Plate Filling eur X Enter Mame SD Enter Delay Time 15 6 9999 sec 29 95 17 If you want the system to perform another file after the present file is complete enter the desired file name and time delay between programs if desired Use the displayed keypad to enter the text of the program to link with in the Enter Name field and select enter Use the displayed keypad to enter the seconds in the delay time field When complete click on Exit Now the Link File field should display the file you linked to and the specified time delay Pause Enter Delay Time Instead of a time delay you also have the option to have a pop up screen prompt you to press continue before
11. 8 1 OVERVIEW M P 9 60 9 SETINGS CONTROLJ vuelos EerUr EedieoSe V OUV dei ii 62 9 1 OVERVIEW cen a ERR ctor estas ecclesia kates shia MC REL MAL AR UM ADSL MALA DIOE 62 WellPro User Manual 01 03 07 V 10 ADJUSTING THE TIP HEIGHT sv oo NE AS NND PROF QUU MPEd seasons 70 10 1 OVERVIEW A a a E rM D Au UL MP ADD D DIAM DD US 70 10 1 1 Editing Running the Position Adjustment Me NU cccceeccceccceecceeccceececeecceees 71 11 LINKING FILES m T 78 11 1 OVERVIEW Soa ater eine PEINE DL OP A LI CON AM sae IL MALA ERI EDDA IRI 78 11 2 USING INKED FILES td dd dd 79 12 OPERATING HINTS AND MAINTENANCE occcccccccccccocococococononocnocnononcccocococononononononononons 80 12 1 OVERVIEW A A A LUAM Lr E A 80 PA A ca aucnd aan leeaecion enna suvatinnenac E Saeed eas edaecces od EERTE 80 12 3 POWER INTERRUPTIONS 255 vx rao uen Erde i ei tet hbri ie 80 12 4 MAINTENANCE sousteseetiitucvantetiet ue Eran tation gown d eere ind 80 12 4 1 Preventive Maintenalie6 cio tts ee a M eU caters eco d MR LC EE co ao Ue 80 12 4 2 Cleanine UP PER TTVTER 81 12 4 3 PISTON PUNTOS aia MERI Dad ita PD p erase usb ete ae ae Ru poit 81 12 4 4 Table Rails o ero a a a A a e Ni 81 12 4 5 Cleaning the Plastic Cover sd ax eed tan ntu O 81 12 4 6 Fuse Replacermelib sd ooo deos Sea RUIN VERE Edi PEORES ds 81 12 5 TROBBBESHOOTING becsdst oerte n RNV EE
12. After clicking on text a pop up screen with numbers will be shown Click on the numbers of desired value and click on enter when finished 74 Calibrating Liquids For liquids that require an accuracy adjustment during volume dispensing you can determine the calibration factor that adjusts to the specific gravity of the liquid The Volumetric Calculation Settings adjusts the movement of the piston motor to improve pipetting accuracy of specified liquids WellPro is factory calibrated for distilled water at room temperature however you can add calibration values to improve pipetting accuracy of liquids with specific gravity that is significantly different from water Baie e nd en mem 235 Backlash A str ir Gar ste Tie vol Disable Buzzer Eject i A tpP Back iaht B min Offset 28 ste Password 1234 aL steps A stp 2 Contrast Max vol Aste Battery 6 66 v Home All Bul s Next IR This is the calibration screen for adjusting settings of pipetting accuracy These settings should only be changed by an authorized technician or designated quality control technician Backlash This setting will compensate for backlash effects resulting from mechanical components To set the backlash setting you must first place a pipette tip on a nozzle Next fill a trough with distilled water and aspirated 5ul To aspirate 5ul perform the following 1 2 3 Click on the Plunger
13. Corporation shall not be liable for any damages whatsoever arising out of the use or inability to use this product Printed in the United States SAFETY SYMBOLS AND MARKINGS These symbols are intended to draw your attention to essential information and alert you to the presence of hazards as indicated Some of these symbols may not appear in the manual or on the product SAFETY SYMBOLS used in the WellPro Model 3000 Liquid Handler Power ON Power OFF O WARNING MARKINGS used in the documentation Caution risk of electric shock Caution biohazard risk Caution risk of personal injury to the operator or a safety hazard to the surrounding area Caution risk of damage to the instrument other equipment or loss of performance or function in a specific application gt gt pD WellPro User Manual 01 03 07 iii ABOUT THE USER MANUAL This User Manual has been written for the user e g laboratory technician and provides information on the WellPro Model 3000 Liquid Handler This manual contains the installation and operating instructions for the WellPro Model 3000 Liquid Handler Read the manual in its entirety prior to operating the instrument This User Manual has been designed to give you the information you need to e Review safety precautions e Install the WellPro Model 3000 Liquid Handler e Use the WellPro Model 3000 Liquid Handler in routine analyses and research e Perform basic maintenance procedures
14. Liquid Handler is shipped in one carton that contains the items listed in Table 2 1 When the system is received carefully unpack the unit and verify receipt of all components We recommend that you save the shipping container and packing material in case you need to move or ship the instrument Table 2 1 PACKING LIST Quantity Description Part Number 1 WellPro 96 Long base WP3000 96 1 WellPro 96 Long base w Single Channel WP3000 96 CP 1 WellPro 96 short base WP3000 96S 1 WellPro 96 short base w Single Channel WP3000 96S CP 1 WellPro 384 Long base WP3000 384 1 WellPro 384 Long base w Single Channel WP3000 384 CP 1 WellPro 384 short base WP3000 384S 1 WellPro 384 short base w Single Channel WP3000 384S CP WellPro 3000 Operators Manual OM3000 Controller Touch Screen 8025 Plastic Cover Tinted 76090 I IL AY Ll Templates for Short Bed Instrument Template for Long Bed Instrument Micro Trough 4 per pack SAMPLE Chilled Reservoir SAMPLE Benchtop lonizer for the 384 Instrument only 4009885 If there is external damage to the shipping carton report the damage to the shipping company and your local ProGroup Instrument Corporation representative lf internal parts are missing or damaged report this to the shipping company and your local ProGroup Instrument Corporation representative as soon as the problem is observed Note If there is any apparent damage to the system the user should investigate the nature 2 5 of th
15. appropriate plates are in position on the deck Once you have confirmed then touch press the OK button 13 45 10 Serial Di lution A OOOO OOOO BDO ODO ICC IO CODO OOO DOO OOO OOOO EO OOO OOO OOOO pil SE CITE OCTET Status Running CO He F Above screen is for WellPro 12 channel 96 well plate 44 Serial Dilution SuL A 000000000 00000 0001010 000000000 00000 a a a nogococonmnocgcoenmnogoongpogo 000000000 00000 a a a 000000000 00000 00000 000 000 O IO O IO IO 00 0000000 010000 a a a 000000000 00000 00017 00 0000000 000 000 0 ICO OO0O0OR DIO IO ICO 000 000 DIO O IO IO 0000000500 00000 000017 ol o ol ol ol ol o ol ol ol o a ol ol ol o 13 40 28 EH Above screen is for WellPro 24 channel 384 well plate The serial dilution will begin with a status screen showing the rows as they are being filled Pressing the STOP button while program is running will cause a pause in the program and pop up a program paused message with option to abort or continue 13 45 41 Serial Di lution E A UR HE EE B bort amp Return Tips Pressing the STOP button while program is running will cause a pause in the program and pop up a program paused message with option to abort continue or abort and return tips Pressing the Abort button will abort the program or pressing the continue button will continue with the program The abort and return tips option will return the tips to the rack and abort the p
16. are designated by letters A through P back to front or A through H The program will start with first tip row specified and if the change tips is YES it will go to change tip row For example The screen above will start with row P of tips and fill row A of well plate It will then eject tips and pick up row O of tips and then fill row B of well plate etc If change tips is set to NO then program will use row P of tip rack to fill all the rows selected for well plate CHANGE TIPS is used to indicate if the tips should be removed after the first liquid is dispensed and a new set of tips from the tip magazine should be placed on the pipette head thus decreasing carryover f you don t want to change tips then the same tips will be used throughout the entire operation Note The above does not track tip rows used If tip row tracking is needed for tasks such as linking programs or if you need to keep track of last row used after running a program so you know where to start with next available tip row then use the following setup below 28 Plate Filling Gaul Xx Actual Values Screen 343 A O EE E Fill pliate E E ET Et Ed n n E 2 K L H r4 CI F rows Change tips So Back 12 18 01 Eu Hele 13 12 35 Plate Filling Actual Values Screen 3 3 Fill plate P D E F G H rus BEBE First Tip Row e nar Ea BR Change tips TES First Tip Row NAT Setting the next First Tip Row
17. e WellPro Reagent Troughs and Pipette Tips 3 2 WellPro WP3000 Touch Screen Controller The WellPro WP3000 Controller includes a touch screen display which provides the operator with complete control of all liquid handling operations The basis of operation is via a user generated method which includes a series of primary operations The method can be stored and retrieved as desired Each primary operations provides for user control of a given aspect of the operation Typical operations include the volumes to transfer mix or dispense the transfer speed and whether to change tips between transfers 3 3 The WellPro WP3000 Liquid Handling Unit The WellPro WP3000 Liquid Handling Unit is an electro mechanical transfer unit that includes a table and a vertical head assembly The table accommodates templates which position reagent containers a magazine with rows of disposable tips etc During operation the table slides back and forth horizontally to bring the appropriate workstation under the head 17 The standard head assembly consists of three basic parts e A plunger housing which contains nozzles that press into the tips to form airtight seals e A plunger block which moves up and down to aspirate and deliver samples e Atip ejector plate which removes used tips The head assembly slides up and down vertically to bring the tips to the appropriate height to perform the designated operations A single channel nozzle for cherry picking i
18. first row in the tip rack Rst NAT is only active when NAT is selected on last screen in the First Tip Row field A GO button exists at the lower right hand corner of every screen This can be pressed at anytime to run the current program loaded into the system 13 29 07 Serial Di lution uL A Source well volume 166 uLL0 2808 hs Description This parameter determines Aow much liquid is in the source well before ang tranfer has made Touch the number to change value The value is anm integer etueen C and 2eee uL E VOLUME IN SOURCE WELL AT START is used to tell the system how much volume is in the initial source row 35 13 29 37 Serial Dilution GuLi A Actual volume in vell 166uLL6 7666 hs Description z This parameter determines ho much liquid is in eccl wel ou before any tranfer has made ee the number to change value e value is am integer etueen C and ze ul EU ACTUAL VOLUME IN WELL is used to tell the system how much diluent is in each well before any transfer has been made This parameter does not pertain to the first row which contains the sample The system uses this number to determine how far down into the wells the tips should go and also to prevent the instrument from attempting to withdraw more sample than is actually present This parameter can be set from 0 to 2000 LL in 1 uL increments Verify the capacity of the plate type you are using is greate
19. from corrosion or salt accumulation To ensure maximal safety and trouble free operation clean up spills immediately Turn off and unplug the WellPro liquid handler before wiping up spills 12 4 3 Piston Plungers On a periodic basis gently apply a light silicone grease to the piston plungers of the 12 or 24 channel liquid head using a clean cloth or lint free towel This will keep them sealing well and inhibit corrosion 12 4 4 Table Rails The rails on which the WellPro table runs must be kept clean and free of foreign matter to ensure smooth operation Periodically clean these by wiping with a clean cloth ALWAYS BE CERTAIN POWER IS TURNED OFF BEFORE CLEANING THE TABLE RAILS 12 4 5 Cleaning the Plastic Cover The plastic covers should be cleaned using a nonabrasive cleaner and then rinsed with a soft clean damp cloth Decontamination may be performed by chemical means Chlorine bleach is an acceptable decontamination solution Ethanol and gluteraldehyde should not be used on the plastic surfaces DO NOT AUTOCLAVE 12 4 6 Fuse Replacement Turn the power switch off and disconnect the power cord from the power outlet before replacing the fuse This procedure will insure against electrical shocks For continued protection against electrical overload and fire hazard replace the fuse only with one of the same type and rating 3 amps 250V Normal blow 12 5 Troubleshooting If you encounter difficulties in the use of the instrumen
20. in increments of 1 or 25 13 0456 Plate Filling uL raf volume at start TY mL Current Wool ure trof 2 mL trof ecmL traf SomL e Cool Trof EE Description This is the volume in the trof integer between Touch the number to change value ess Track t us MICRO TROUGH VOLUME AT START is used to indicate how much liquid is in the reservoir you have chosen before any liquid has been removed The system uses the delivery volume and the number of rows to be filled to calculate the minimum volume needed to run the file and indicates it on the display as the lower limit You can set the value of this parameter in 1 mL increments from this lower limit to 20 mL 58 mL or 77 mL for the front middle and rear reservoirs respectively for the MICRO TROUGH reservoir The Cool Trof can hold 100 mL Enter the amount of liquid volume present in trof at start of the program and press OK Note If tracking of volume of liquid in trof during change of programs or linking of programs then set Track on See screen below 132554 Plate Filling So X Trof volume at start Current Wool ure trof 22 mL trof ecmL trof SomL a Cool Trof hs Description This is the volume in the trof Touch the number to change value The value is an integer between 1 and 19 mL Press Track the remaining volume 25 Pressing the Track button will turn and activate the tracking volume feature of software on
21. number to change 9wvalue The value is an integer etueen CU and See uL Eli Hele MICRO TROUGH MIX VOLUME is used to indicate how much liquid to aspirate into the tip and dispense back into the MICRO TROUGH in order to mix the MICRO TROUGH contents This parameter can be set from 0 to 200 uL in 1 uL increments 0 indicates no mixing for 12 channel head and O to 50 uL in 1 uL increments for 24 channel head 13 00 42 Plate Filling Delivery volume 166 ul 6 266 OK EE Description J This parameter Sos Ran much liquid to di ense nto eccl from the MICA TROF Touch the number to change value The value is an integer etween QC anda See xL FI He r DELIVERY VOLUME indicates how much solution should be dispensed into each well from the MICRO TROUGH reservoir This parameter can be set from 0 to 200 uL in 1 uL increments for 12 channel head and 0 to 50 uL in 1 uL increments for 24 channel head 23 o5429 Plate Filling ouL E X dE x Description HYou can zpecifu mixing mof H tle well contents after A tranzfer Touch the number to change value The value is an integer eiueen c Zero indicates NO mixing Eu Cele MIXES AFTER TRANSFER is used to specify the number of mixes after the aliquot has been delivered to the plate This parameter may be set from 0 9 in increments of 1 0 indicates no mix after transfer 13351 Plate Filling eur ES actual Values Screen 2 3 Plate orientat ion
22. starting the next program This is done by entering the number 9999 which will create the pause before proceeding command 26 13 08 38 Plate Filling Gull A Actual Values Screen 34 3 Fill plate E b E F amp H rus HBB First Tip Row H al el Change tips 13 10 11 Plate Filling Actual Values Screen 3 3 Fill prote B IRR a d A I ros m IL e Change tips TES S Gack The above display is for the WellPro 12 channel 96 well plate in landscape or portrait Plate Filling Gull X Actual Values Screen 343 E O F G H ru cos A a D0Q0HQ Q amp EGOHGE First Tip Rou e P E e Change tips NN Back mm aie EB Helper m gt The above display is for WellPro 24 channel head 384 well plate 27 FILL PLATE ROWS is used to indicate the rows of the plate to fill and which to leave unfilled Rows are designated by letters A through P for 384 well plates and A through H for 96 well plates To select row A press the A button which will then fill in with red If you want all rows to be filled press each button For rows that you don t want filled then do not press the button with corresponding row letter Non filled rows will have no red filled in on the box Actual Values Screen 3 3 Fill plate P D rous EIEIEIEIEIEIEIEH Change tips TES soi Bec E ENTER FIRST TIP ROW is used to specify which row of tips to use Rows of the tip magazine
23. to L De run the application L De Mo AA a rial EAR Next INE Touching Pressing the GO button will pop up a message to confirm that the appropriate plates are in position on the deck Once you have confirmed then touch press the OK button 131958 Plate Filling cul EA E A O E OOOO A CRT LLL Status Running EB Help mm The above display is for the WellPro 12 channel head 96 well plate 30 Plate Filling Gaul x cono muinin cono cono cono cono misin cono cono cono misin ooo cono cono cono misin 000000500 00000 0001017 00 0000000 000 0000 a a a P Status Running pela ets B Help The above display is for the WellPro 24 channel head 384 well plate The plate filling will begin with a status screen showing the rows as they are being filled Pressing the STOP button while program is running will cause a pause in the program and pop up a program paused message with option to abort or continue or abort and return tips 13 20 34 Plate Filling O E E eee a AN Program Paused Lu T dacmrmamsc usi Pressing the STOP button while program is running will cause a pause in the program and pop up a program paused message with option to abort continue or abort and return tips Pressing the Abort button will abort the program or pressing the continue button will continue with the program The abort and return tips option will return
24. to NAT will keep track of next available tip that has not been used This should be set at NAT if you are linking programs or wanting to keep track of next row of tips to be used while manual running programs If you want to reset the next available tip row to the beginning then at screen one press Rst NAT See below When you press Rst NAT located on screen one a Pop Up screen will be displayed asking to make sure the deck is OK If you press OK another screen will Pop Up asking Change the magazine and select the next tip row Next tip row Note Changing magazine is not required if you would like to continue to use same tips CHANGE TIPS is used to indicate if the tips should be removed after the first liquid is dispensed and a new set of tips from the tip magazine should be placed on the pipette head thus decreasing carryover If you don t want to change tips then the same tips will be used throughout the entire operation 29 1334828 Plate Filling eu X Actual Ualues Screen 1 3 Volume in wel 8 uL Deliver from a L20nil 1 Mixes in Trof 3 rof mix volume 2688 uL Delivery volume e 188uL Nixes after transfer 2 uille Next INR Eu Cele Buses Plate Filling cuLA A Actual Ualues Screen 1 3 Volume in uell amp amp uL De Y 7 Hi Nake sure the deck Tri is set Press UK
25. used the WellPro liquid handler stops automatically and displays the ENTER FIRST TIP ROW prompt again Change the magazine and specify the row of tips that you want to start with Pause Enter Delay Time Instead of a time delay you also have the option to have a pop up screen prompt you to press continue before starting the next program This is done by entering the number 9999 which will create the pause before proceeding command 79 12 OPERATING HINTS AND MAINTENANCE 12 1 Overview The WellPro 3000 Liquid Handler is designed to be used with a minimum of maintenance In this chapter we describe routine operations that can be performed by the operator cAUTION CAUTION Do not open the Backpack Electronics or disassemble the controller unit There are no operator serviceable components in the instrument other than those describe in the SERVICE AND MAINTENANCE and OPERATING INSTRUCTIONS sections WARNING WARNING For protection from injury by the WellPro liquid handler moving parts Do Not Remove the blue plastic cover while the WellPro liquid handler is operating or the power is on 12 2 Tips The WellPro liquid handler has been calibrated using WellPro 96 or 384 disposable tips The design of the tips and the manufacture of their internal volumes orifice diameters lengths etc to tight tolerance limits assure the accuracy and precision of the liquid handler system The length of the tips is of particular importance since the
26. 6 268668 ES Description S This parameter determines lh eu much liquid is im each well before any tranfer has made ee the number to change volue e value is a integer etueen cC ang ze UL EB Help a uil VOLUME IN WELL PLATE 2 is used to indicate how much liquid is in each well of plate 2 The system uses this value to determine how far down into the wells the tips should go and also to prevent exceeding the volume capacity of the wells eds ag Plate to Plate Sul A Actual Ualues Screen 144 Transfer Direct ion e P2 To P1 Volume in vell Plate 1 B uL Volume in vell Plate 2 e 168 uL Transfer Mode e Several Tanster Yo lume 4 58uL siWexi Def Values Nest EB Helper TRANSFER MODE is used to tell the system whether to transfer 1 to 1 one row of plate 1 to the corresponding row of plate 2 or to transfer one row of plate 1 to several different or multiple rows of plate 2 50 13 52 22 Plate to Plate Transfer volume 56 uL 6 766 Hype Description This parameter determines how much liquid ta transfer fram the source plate Touch the number to change wal ue The value is an integer etween C and ze uL TRANSFER VOLUME is used to tell the system how much sample to withdraw from a source well for transfer to a new well during a plate transfer This parameter can be set in 1 uL increments The system calcula
27. LLPRO 3000 LIQUID HANDLER cccccccccccccccccsccccccscsccccsccceecs 16 3 SYSTEM COMPONEN g m mmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmrmrmmmmmmkcky rimmmmmmmmmrmmmmmmmmmmmmmmmmmmiiiymmmmccrmmrmaHNI 17 3 1 INTRODUCTION rra ii ansias 17 3 2 WELLPRO WP3000 TOUCH SCREEN CONTROLLER cccccccccccccccccccccccccsccccscvccccscvccsceccecscesvecs 17 3 3 THE WELLPRO WP3000 LIQUID HANDLING UNIT ccce e e nn hh he e eher esee hereto 17 3 4 THE WELLPRO REAGENT CONTAINER cecccccccccccccccsccccccsccccscecvccececcccscescccecvscscecesesceceecs 18 4 USER INTERFACE cta cuit tora tese sent ine deed Anl n oa E MEL UA RED DS 19 4 1 TOUCH SCREEN INTRODUCTION mmmmmmmmmmmmmrmmmmmmrmmmmrmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmHH 19 D PEATE SIBI com o 20 5 1 OVERVIEW dae rbat sc ine a o CLIE se a Uo ED PAL MALA ee 20 5 2 CREATING AND EDITING A PLATE FILLING FILE cc cece ccc ccc c ccc cccccccccccccececcccsccccccscvccscecesesees 20 6 SERIAL DILUTION ts leads 34 6 1 OVERVIEW smod eccL A A end A IU uo CUAL M ALII P DM MAL D E 34 6 2 CREATING AND EDITING A SERIAL DILUTION FILE cc cece cccccccccccccccccccccccccecvccecvccccscecvscscecs 34 F PLATE TO PEATE TRANSFER coito ia das 47 7 1 E 47 7 24 YPES OF PLATE TO PLATE TRANSFER rs dd dis 47 7 3 CREATING AND EDITING A PLATE TO PLATE TRANSFER FILE cccccccccccccccccscccccccscccccccccceccsceceecs 48 8 HOST CONTROL AND MANAGING FILES coincida is 60
28. NE tado OE A DIE 81 WellPro User Manual 01 03 07 vi 1 INTRODUCTION 1 1 Overview THANK YOU for purchasing the WellPro 3000 multichannel automated pipetting workstation We are proud to send you our flexible 12 or 24 channel automated workstation which is capable of performing various routine liquid manipulations It is an intelligent alternative to tedious and potentially error prone manual pipetting operations Now applications such as reagent addition serial dilution microplate replication and sample dilution can be easily performed The ProGroup Instrument Corporation WellPro WP3000 Liquid Handler Figure 1 1 is designed to perform the following liquid handling activities on a user programmable basis e Precisely transfer liquids in volumes from 1 uL to 50 uL using disposable tips with the 24 channel head e Precisely transfer liquids in volumes from 1 uL to 200 uL using disposable tips with the 12 channel head Perform serial dilutions in almost any ratio Perform serial dilutions of 16 wells or 24 wells simultaneously with 24 channel head Perform serial dilutions of 8 wells or 12 wells simultaneously with 12 channel head Precisely mix the contents of a microplate Transfer samples to or from microplates Perform mother daughter repli plating Perform well to well transfer Cherry Picking Optional Feature and is not standard w instrument Fig 1 1 ProGroup Instrument Corporation WellPro WP3000 Liquid Handler
29. Reagent Containers The WellPro liquid handler can be used with the MICRO TROUGH reagent container each of which consists of three reservoirs Each reservoir holds a different amount of liquid e g 20 mL 58 mL and 77 mL You can use the reservoirs to hold reagents that will be added to a plate or waste that has been removed from a plate The WellPro liquid handler can be used with a 100 ml chilled reagent container You can use this reservoir to hold reagents that does or does not required chilled The trough also has disposable liners as an added benefit Chilled Trough Disposable Liner 1 2 4 WellPro Templates The WellPro WP3000 liquid handler uses a template to position microplates reagent containers and tip magazines on the movable table Standard templates provided e Standard Template for WellPro with a Long Bed One general purpose template is used for WellPro long bed liquid handling operations The well plates can be orientated with the 96 well plates 12 well rows A H or 384 well plates 24 well rows A P landscape as shown in Figures 1 2 1 3 1 4 1 5 With the same template the plates can be placed in a portrait orientation 96 well plates 1 12 or 384 well plates 1 24 well columns as shown in Figures 1 6 and 1 7 E i En ICR Pg a I m f 1i Lua b a Ao Ip Rack 46 or 364 ips Landscape Orientation Shown Deck Position 2 96 or 364 well plate in Landscape Orientation Position also used fo
30. STINATION rows DESTINATION ROW is used to indicate which row of the DESTINATION plate is used to place liquid in during transfer This parameter only appears if the 1 to SEVERAL mode is selected in this file In that instance the SOURCE row specified acts as the single source row for transfer to several receiving or DESTINATION rows Plate to Plate Transfer B uL Actual Values Screen del Hake sure the deck is set Press OK to run the appl ication zie une Touching Pressing the GO button will pop up a message to confirm that the appropriate plates are in position on the deck Once you have confirmed then touch press the OK button 56 Plate to Plate Transfer A OU Above display for WellPro 12 channel 96 well plate nogogcoegoogaoemoegcaemoigaamagoegcaongmpog aaa lada 010 10 10 G G III 000 I OO UI IO O IO IO ood o col 0 00 O G fala da D00 DDD G G G D O G O G O G G O O G r 7 ae 1 Above display for WellPro 24 channel 384 well plate The plate filling will begin with a status screen showing the rows as they are being filled Pressing the STOP button while program is running will cause a pause in the program and pop up a program paused message with option to abort or continue 5 Plate to Plate Transfer eu Bs A ooaoananaoanooonaocoaoananoanao nogcgcogogocgoaoemogcaempiganmgogoogog o ol ol ol o ol ol ol D o ol ol 20 05 41 Ely Hel P Pressing the STOP button wh
31. WellPro 3000 Liquid Handler ProGroup Instrument Corporation i E UN Ae ea UN H xA ems l y T i fi l a o 1 MM i ia as ao CE i LL ml gt il hs l T SN d i Bas IA TNI A I Lig dui P M a a i Wel IL a7 dai is em i b i ii a Omur rmz xS LII a A 5 E o Li b WellPro 3000 Liquid Handler User Manual User Manual Rev 2 4 February 18 2007 Copyright Copyright 2005 ProGroup Instrument Corporation All rights reserved Reproduction of the accompanying user documentation in whole or in part is prohibited Trademarks WellPro Automated Liquid Handling System is a registered trademark of Progroup Instrument Corporation All other trademarks and registered trademarks are property of their respective holders Disclaimer ProGroup Instrument Corporation reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy ProGroup assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions No liability for consequential damages ProGroup Instrument
32. ack PLATE ORIENTATION is used to indicate how the plate is positioned The value that is selected indicates the number of wells that will be under the pipette tips at one time A H for 96 well plate landscape orientation 1 12 for 96 well plate portrait orientation A P for 384 well plate landscape orientation 1 24 for 384 portrait orientation 40 EMPTY LAST ROW TO is used to tell the system where to place the extra volume of liquid transferred into the last row of wells after a serial dilution 1 2 If you select OFF the extra liquid will be left in the last row of wells If you select 1ST ROW the extra liquid will be removed from the last row of wells and return it to the first row of wells you chose in ROWS TO DILUTE If you select MAGAZINE the system will remove the extra liquid and discard it into the tip magazine If you select MICRO TROUGH the system will remove the extra liquid and discard it into the rear reservoir of a MICRO TROUGH container located in the P2 cavity of the template lC Note MICRO TROUGH will appear on the display only when the 12 or 24 wells landscape across PLATE ORIENTATION selected If performing a serial dilution in portrait then this option is not available LIQUID HANDLING SPEEDS is used to select the rate sample delivery from the tips rapid medium or slow 13 48 41 Serial Di lution Actual Values Screen 273 Plate Type FLAT Plate orientation
33. an example if you are diluting cells which may settle the MIX BEFORE TRANSFER step might be used to agitate them back into suspension before transfer by mixing This parameter can be set from O to 9 0 indicates no mixing 37 IS EI em Serial Di lution Nixes after transfer 3 8 9 ki Description You can speci fu mixing of the well contents atter an galiquat is transfered Touch the number to change value The value is an integer Between 3 Zeroa ingicates MO mi insg MIXES AFTER TRANSFER is used to indicate the number of aspiration delivery cycles that should be performed after transferring a sample This parameter can be set from 0 to 9 0 indicates no mixing is sa ia Serial Di lution Hell mix volume S uL 8 188 hs Description This parameter determines the volumne to mix the contents of the well before transfer Touch the number to change value The value is an integer etween C and See ul MIX VOLUME is used to tell the system how much solution should be used in the Mix step Typically this volume is 50 of the total volume in the well to be mixed but will be more or less depending on the application when mixing cells it may be desirable to use a lower volume of the total volume 38 13 32 53 Serial Dilution ouLE A Actual Values Screen 2 3 Plate Type FLAT Plate orientat ion 5 A H Empty last rou to e UFF Liquid han
34. ate for assay culture etc The Plate To Plate button is used to establish a user file which can be stored and used as desired 13 49 26 Plate to Plate A Actual Values Screen 14 Transfer Direct ion e P2 To P1 Volume in vell Plate Fe 6 uL Volume in vell Plate 2 e 166 uL Transfer Mode ae 1 TO 1 Tanster Yo lume ex 58uL Sexi Def Values Nest ie Press the Def Values button to change back to system default values 13 49 51 Plate to Plate A Actual Values Screen 244 Mixes before transfer Nixes after transfer nix Yo lume _ Liquid handling speed Change Tips _ Above display for WellPro 12 channel 96 well plate 48 Plate to Plate Transfer oul X Actual Ualues Screen 14 Transfer Direct ion e P2 To P1 Volume in vell Plate 1 a uL Volume in vell Plate 2 e 56 uL Transfer Mode __ e 1 TU 1 Tanster Yo lume e e 25uL E i Def Values Next Hele Above display for WellPro 24 channel 384 well plate 19 32 11 The TRANSFER MODE DIRECTION is a feature that is especially useful when it is combined with FILE LINK because it allows you to transfer duplicate samples from the rows of one plate to another plate Thus using these functions in combination makes it possible to
35. ch controls the software commands 60 Implemented Serial Commands Initialize the WellPro machine It s also highly recommended HOME Arg HOME ALL r HOME ALL OK r that you use it inside your programs to update the remaining volume in the controller and avoid float calculation s offset It makes an absolute movement in the specified MOVE X 125 2 OK r axis Arg X Y Z ALL MOVE Arg Pos Spd Arg X Y Z Pos 0 to axis limit Spd 1 4 FILL_TIPS FILL_TIPS r FILL_TIPS OK r Fills the tips to maximum volume from any previous position HOME_TIPS HOME TIPS HOME TIPS OK Nr Dispense all the remaining volume in the tips from any previous position WITHDRAW value WITHDRAW 50 WITHDRAW 50 OK r This command indicates to the WellPro the amount of liquid to withdraw in uL Value must be an integer number in the range of 1 to 200 This command indicates to the WellPro the amount of liquid to dispense in uL Value must be an integer number in the range of 1 to 200 REMAINING REMAINING r Returns the remaining volume in uL PICK_TIPS col mag PICK_TIPS 3 PORT r PICK_TIPS 3 PORT OK r The WellPro will pick up the Column 1 8 tips from the specified column Magazine LAND PORT You must specifie the orientation of the tip magazine LAND or PORT EJECT_TIPS column EJECT_TIPS 2 r EJECT_TIPS 2 OK r The WellPro will eject the tips Column 1 8 to the specified column When Column 0 eject only co
36. ction The basis of operation of the ProGroup Instrument Corporation WellPro 3000 Liquid Handler is the file which is a set of instructions and conditions that are used to perform the desired operation The operator can establish store and execute files via the touch screen controller module The WellPro user interface consists of a touch panel that activates the liquid crystal display LCD From this touch panel all program modes and variables can be easily accessed and adjusted To pause a program simply press the STOP Icon on the touch panel during a run Touching the GO Icon resumes the program All modes commands and data input operations are accomplished using the touch panel The instructions and prompts are in dialog format Variables can be changed by touching the text highlighted in blue ProGroup FroGiroup Instrument Corp WellPro Autamated Liquid Handling system Mum cu SS Figure 4 1 When the system is powered up the display will be as shown in Figure 4 1 Touching the Main Menu button will pop up the main menu screen shown below At the main menu screen you have the option of selecting a process and creating a file with your parameters and saving the file 19 Hain Menu Plate Ser ial Plate Filling Dilution To Plate Cherry Picking settings 2 53 19 5 PLATE FILLING 5 1 Overview Plate filling refers to the addition of a user specified quantity of liquid to the wells in a
37. d Rate 9600 Parity None Data Bits 8 Stop Bits 1 e Before performing the procedure the first time you need to setup the Hypterminal parameters located on your computer Go to start menu All programs Accessories Communications then choose Hyperterminal A popup up menu will now appear for you to enter a name for this You can enter a name such as WellPro and continue or give it no name to continue Now you should see the following popup menu 64 Mew Connection HyperTerminal e Hyperterminal New Connection Properties a New Connection Phangan L ountry regior United States 1 H Enter the area code without the long distance prefis Area code Connect using Conexant HDA D110 MDC W 92 b se Conexant HDA D110 MOC 92 Mode COM TCP IP Miingock Use country region code and area code L Redial on busy uw 65 wellpro Properties Connect To Settings Function arrow and ctrl keys act as Terminal keys Windows keys Backspace key sends CH Del C2 Ctrl H Space Ctrl H Emulation ans Telnet terminal ID VT10U Backscrall buffer limes 500 E Play sound when connecting or disconnecting Input Translation ASCII Setup wellpro Properties Connect To Settings ASCII Setup Function arrow and ctrl keys act as Terminal keys C Windows keys ASCII Sending Send line ends with line feeds Echo typed characters locall
38. dilutions As an example if equal volumes of sample and diluent were mixed during each step of the series the sample concentration would be 1 2 1 4 1 8 1 16 1 32 and so on in sequential rows Hain Menu Plate Serial Plate Filling Dilution To Plate Cherry Picking sett ings 2 53 19 6 2 Creating and Editing a Serial Dilution File At the main menu screen you have the option of selecting a process and creating a file with your parameters and saving the file Pressing the Serial Dilution button will open up a screen for changing the serial dilution parameters eim Serial Dilution cuLA A Actual Values Screen 1 73 Volume in source wel Volume in uel a Transfer Vo lume _ Nixes before transt ere s Nixes after transfer s Nix volune e Sexi t Def Values West EE Help Press the Def Values button to change back to system default values 34 Serial Dilution SuL kK Actual Values Screen 143 Volume in source uell e Volume in wel Transfer Volume e Nixes before transfers Nix volume Plate Type ae a Exit Def Values bet PSS eS E Pressing the text values parameters in blue will lead to other screens shown below so that parameters can be changed Rst NAT Reset next available tip row Pressing this will reset the next available tip row to the
39. dling speed e High Change tips e Link files T Back Next Serial Dilution cul kK Plate Type ki Description This parameter deternines the bottom shape of the E cre Miele EH PLATE TYPE is used to indicate the design of the bottom of the plate V shape U shape Flat This information is used to determine the height of the liquid in each well and thus calculate how far down into the wells the tips should go If you are not using a standard microplate select custom for one of the ten custom plate types available The custom programs for non standard plates are configured in the settings configuration from the main menu In the settings menu you can set these parameters and determine the amount to raise or lower the tip height The height parameter can be set from 100 to 3500 in increments of 1 or 25 39 13 41 28 Serial Dilution cuLA A Empty Last Row ta UFF ki Description This parameter determines uliere to place the extra val ume transferred into the last row E Empty Last Row to This parameter determines where to place the extra volume transferred into the last row 13 40 41 Serial Dilution uL A Actual Values Screen 2 3 Plate Type FLAT Plate orientat ion A H Empty last rou to e UFF Liquid handling speed e High Change tips _ Link file x WH B
40. e damage before plugging the unit into the mains to ensure that powering up of the system will not create a hazardous condition or damage internal components If the damage appears significant call your local ProGroup Instrument Corporation representative before connecting the unit to the mains Initial Installation of the WellPro 3000 Liquid Handler Assembly installation and final inspection of the WellPro 3000 Liquid Handler will be done by the user To install the unit 1 Connect the Touch Screen Cord to the back of the WellPro and slide the Touch Screen onto the bracket connected to side of the unit 2 Place the power cord to the line into the socket into the rear panel of the liquid handling unit and plug the unit into the line 16 WellPro Autamated Liquid Handling System Wo c iE Figure 2 1 The Controller Screen when Powered Up Power up the unit The liquid handler will initialize and the controller will present Figure 2 1 The pipette tip head will move in the Z upward direction and the table will move in the Y outward direction to determine the zero If the head does not move contact your local ProGroup Instrument Corporation service representative for assistance 3 SYSTEM COMPONENTS 3 1 Introduction The ProGroup Instrument Corporation WellPro 3000 Liquid Handler system includes the following components e WellPro Controller Section 3 2 e WellPro WP3000 Liquid Handling Unit Section 3 3
41. en out compromising accuracy To avoid this use the mix option prior to a programmed aspiration to equalize the air in the tips before pipetting the liquid Proper vapor pressure will be maintained even if the device is left idle with filled tips for a prolonged period Tip Immersion is important to ensure that pipet tips are properly immersed in liquid before aspirating Tips should be immersed just below the level of the liquid and the depth should be consistent throughout the pipetting cycle This will prevent air from being aspirated into the tips as well as avoid extra liquid adhering to the outside of the tip 3 4 The WellPro Reagent Trough The WellPro liquid handler is used with a Micro Trough which is a reagent container The Micro Trough container consists of up to three reservoirs each of which is used to contain a reagent or solution In some cases fluid from the wells is deposited in the Micro Trough The WellPro also uses a 1 ml chilled trough with disposable liners for reagents that require to be kept cool during transfer The WellPro WP3000 liquid handler uses templates to position microplates reagent containers and tip magazines on the movable table Note that all WellPro s can fill plates in landscape mode but the long bed WellPro is the only instrument that can fill plates in both landscape and portrait orientation The short bed can not fill in portrait orientiation 18 4 USER INTERFACE 4 1 Touch Screen Introdu
42. ep to allow the droplet to contact the bottom of the well Air Gap The air volume should be sufficient to assist the separation of the droplet from the tip to the well bottom but not so great that air bubbles become a problem The air gap is parameter that is often optimized by trial and error Approximately every 20 steps 1ul of air gap for 24 channel head and 8 steps ul of air gap for 12 channel head 77 Single Channel Head DISABLED 4th fixis Offset 8 0 o ESH Vol Adj 20 ul Pos fd j 99 Turn Gt after ED SEE Home Al Enable or Disable WellPro Supplies 200 ul Tips SO racksfcase Pana TOU 200 ul Tips Sterile SO racks case______ Part TOUSU2 120 ul Tips Sterile Filter S racksicase Part TOOSOS 50 ul Tips Man Filter 50 racksicase Part TODG04 50 ul Tips Sterile 50 racksicase_ _ Part TOUSOS 3l ul Tips Sterile Filter 50 racks case Part TODSO06 30 ul Tips Sterile Mon Filter SO racksicase_ Par TODSO 3l ul Tips Non Sterile 50 racks case Partz TOOSO0S MicroaTroughs Sterile 48 troughsicase Part MTOUSU2 WellPro Support screen can be accessed from the support button located in lower right corner of the main menu This screen lists available pipette tips and troughs with corresponding part numbers Please use these part numbers when ordering supplies 11 LINKING FILES 11 1 Overview The FILE LINK feature is provided to allow you to join
43. er housing which contains nozzles that press into the tips to form airtight seals e aplunger block which moves up and down to aspirate and deliver samples e atip ejector plate which removes used tips The head assembly slides up and down to bring the tips to the appropriate height to perform the designated operations The WellPro WP3000 can be configured with a 24 channel tip air displacement pipetting head or a 12 channel tip air displacement pipetting head Serial dilutions can be performed in the 16 or 24 channel mode with 24 channel head to allow for maximum dilution capability with the 384 well plate or can be performed in the 8 or 12 channel mode with 12 channel head to allow for maximum dilution capability with the 96 well plate An optional single channel well to well cherry picker head can be added to the instrument The single channel head can perform well to well transfers and dilutions in a 1 200 ul range for the 96 WellPro and 1 50 ul for the 384 WellPro This module uses an excel spreadsheet and requires a computer to operate 12 or 24 Channel Liquid Head s 2 LU OC E a a i LIB E P i I Pu En iste E Es i Interchangeable Template for snort bed WellPro and a single Ie Template for the long bed sliding Table that moves forward and backward Standard WellPro Optional single channel cherry picking head not shown TIU III 12 Channel Liquid Head 24 Channel Liquid head 1 2 3 WellPro
44. erial dilution reagent addition Serial dilution reagent addition mixing plate replication sample mixing plate replication transfer plate to plate and within sample transfer plate to plate plate and within plate Technical Specifications Dimensions Controller Height 6 0 Width 9 5 Depth 8 5 Weight 3 Ibs Power Requirements 120 220 240 VAC 47 63 Hz 2A 120 VAC 1A 240 VAC Fuse 3A normal blow Operating Temperature 23 3 C 68 79 F 82 83
45. ext Folder EA Documents and Settings Aussell Press Start Go to Touch Screen and into Manage Files Click on Download Settings to PC IMPORTANT WAIT FOR THE END COMMAND TO SHOW IN HYPERTERMINAL MENU AND LISTEN FOR A BEEP sound could take a couple of minutes before you continue Now press capture text and then Stop very important now you can disconnect and close the Hyperterminal menu e For downloading the customer backup program files to Wellpro repeat the same process used for downloading the settings except for now click on Download all files to PC in the manage user file section of touchscreen 10 ADJUSTING THE TIP HEIGHT POSITION ADJUST SECTION 10 1 Overview The depth of the tips in the wells must be properly set in order for the system to accurately aspirate fluid When standard microplates are used this depth is set by choosing one of the preset values FLAT U or V that is presented in the PLATE TYPE parameter in the various files However when other plates are used in a few special situations it will be necessary to manually set the tip height using custom file in the settings menu under the position adjust section 70 Actual Rack Magazine Land aj ka Save current position as Deck pos B Tire Bottom Tie Sate Ba Tig TOP I Orient LAND Well wal I a uL e LEE This position adjust screen allows for settin
46. fer has been made This number is used to determine how far down into the wells the tips should go and also to prevent the instrument from attempting to add more liquid than each well can hold This parameter can be set from 0 to 2000 uL in 1 uL increments Do not exceed the volume capacity of the plate type you are using ie 350 uL in a standard 96 well flat plate or 1 2mL in a 1 2mL deep well plate etc 21 12 58 08 Plate Filling cuLA A Deliver from Trof 88mL Trof zeomLidTrof amp eomLgTIrof SocmL en els Wreck EE Description This parameter determines Whether it should aet solution from the trof Il He lr DELIVER FROM is used to indicate the MICRO TROUGH reservoir that contains the liquid to be dispensed 155255 Plate Filling Gull A Mixes in trof 3 8 9 ES Description You can speci u mixing of the TROF contents before an aliquot is taker Touch the number to change woalue The value is an integer eiueen C and A Zero indicates NO mixing Eu Cele MIXES IN MICRO TROUGH is used to specify mixing of the MICRO TROUGH contents before an aliquot is taken Mixing of a MICRO TROUGH is especially important with cells or other inhomogeneous samples This parameter can be set between 0 and 9 0 indicates no mixing 22 12 59 54 Plate Filling Trof mix volume 266 ul 8 788 ka Description This parameter determines hou much liquid to aspirate into the tip and dispense into the traf Touch the
47. formance for Small Volumes Precision and accuracy can be difficult to obtain when pipetting small liquid volumes such as 1 0 pL 76 Critical parameters affecting performance included Pipetting speed slower is better for small volumes Plate height tip should be 0 1 0 3 mm above the well bottom Slower pipetting speed prevented droplets that formed at the ends of the tips from contacting the sides or top of the wells as the plate was raised Optimal plate height ensured that the droplets made contact with the well bottom and were removed from the tip during the dispense step Piston Speed Slow piston speed is recommended for small volume aspiration and dispensing Plate Height Plate height requires some trial and error to determine the optimal distance from the well bottom A height that places the tips too deep in the wells will seal the tip to the well bottom and not allow the liquid to leave the tip If the tips are not deep enough the dispensed droplet will not make contact with the well bottom and will not remove the droplet from the tip see the following diagrams 4 Pip tip Papel tip al d 1 0 mm Position of the tip in the plate before dispense Position of the pipet tip during dispense of Liquid and air blowout air gap When positioning the plate under the tips for a small volume dispense allow approximately 0 1 0 3mm of space above the well bottom prior to executing the dispense st
48. g of positions for non standard plates This menu also allows for manual positioning of the head and deck positions 10 1 1 Editing Running the Position Adjustment Menu You can change the value of each parameter as desired to provide the desired program to fill the plates Positions can be moved at one or at twenty five microsteps at a time Caution Only change settings for Custom under actual rack Do not change settings for any other type of plate under the actual rack options All other actual rack options besides custom are set at the factory and changing could result in damage to the instrument and misalignment during operation For setting positions of your non standard plate Press Home All button will home the machine Place non standard plate onto deck of Wellpro Manual press a pipette tip onto nozzle one of liquid head Choose the custom plate number you want associated with this plate Have option to choose from 1 to 10 custom files 5 Select land landscape or Port portrait for the well plate in the Orient button JA WYN 71 6 Using the arrow keys press left or right until deck moves the plate so the pipette tip on head is aligned to center of the plate well When aligned then press the deck pos button in the save current position as and enter the deck position value 7 Using the arrow keys press up or down arrow to move the tip to the top surface of the well plate Then press the tip top butto
49. ile program is running will cause a pause in the program and pop up a program paused message with option to abort or continue Pressing the Abort button will abort the program and pressing the continue button will continue with the program Plate to Plate Transfer PuL A Enter Name PP4 CEE Cancel Save DRDS 9T T aa aja c e r e n1 t w o aja s r v v u x Y 2 eee Jos Jae enter l 20 08 44 EIE He lr After setting the variables to desired value go back to screen 1 of the plate to plate transfer menu and press the Save button to save the parameters Enter the desired file name in the Enter Name field and click on enter button then the save button The save can be performed after setting the variables and before pressing GO to run the program Or the program can be saved after running the program by pressing the back button to screen 1 of the plate to plate transfer menu and then press save button 58 Plate to Plate Transfer ou B A Select File Be Description llse the arrows to find the file Fress CLoqad to load the file Fress Cancel to exit i uu GO ui fee Help To load a saved program press the load button and then use the up or down scroll button to select the program to run 135751 Plate to Plate eut X Actual Ualues Screen 14 Transfer Direct ion e P2 To P1 Volume in vell Plate 1 uL Volume in vell Plate 2 e 168 uL Transfer Mode
50. istance Adjusting this setting can result in damage to the liquid head Offset Is for adjusting volume for viscous liquids Contact ProGroup technician for technical assistance Max Vol This will set the upper liquid calibration limit The max volume should initial be set at 1000 for a 50ul tip 24 channel head and 4000 for a 200ul tip 12 channel head and then press home all Now use the plate filling routine to fill with volume of 50ul 24 channel or volume of 200ul 12 channel then use a gravametric method or reader to determine accuracy Determine the percentage difference from the actual result to the theoretical value Now reduce or increase the 1000 for 50ul tip or the 4000 for a 200ul tip setting by the same percentage difference Run the plate routine again with 200ul or 50ul and record the results The results should now be in acceptable range 1 ul Steps This will set the lower liquid calibration limit To calibrate this setting the value should be changed to 8 steps for 12 channel head and 20 steps for 24 channel head Now use the plate filling routine to fill with volume of 1ul and then use a gravimetric method or reader to determine accuracy Determine the difference from actual result to the theoretical value If the liquid is low then increase the motor step by 1 if the liquid result is high then decrease the value by 1 step Run the plate routine again and record the results Repeat process until acceptable results are produced
51. lumn 0 the machine will no eject the tips in it s actual motion position Magazine LAND PORT You must specifie the orientation of the tip magazine LAND or PORT DELAY SEC value DELAY_SEC 5 r DELAYSEC 5 OK r Causes the program to pause for the number of seconds Delay for the number of specified Seconds must be a seconds specified whole number in the range of 1 to 1x10 MOVE X 125 2 r Value 1 200 DISPENSE value DISPENSE 41 r DISPENSE 41 OK r Value 1 200 61 DELAY_MS value DELAY_SEC 5 r DELAYSEC 5 OK r Causes the program to pause for the number of milliseconds Delay for the number of specified Milliseconds must be milliseconds specified a whole number in the range of 1 to 1x108 RATE speed RATE 2 OK r Changes the withdraw and dispense speed 1 is the lowest Speed 1 4 speed and 4 is the highest speed RATE BEN Uo Rem the current rate speed Returns an integer number Uo Rime 1 and 4 BEEP value ES BEEP 350 OK r Causes the controller to emit a beep sound for the number of Causes the controller to milliseconds specified beep for the number of milliseconds specified WARNING WARNING r WARNING OK Wr Causes the controller to emit a 3 beep tone Useful as an attention getting warning signal 9 SETTINGS CONTROL 9 1 Overview Settings section allows the user to set the internal clock and alter the settings for serial port configuration It also allows
52. n in the save current position as and enter the value shown for the head position 72 Using the arrow down key move the pipette tip head position until the tip touches the bottom of the well Use caution when moving the head down with arrow key when nearing the bottom of well use the microstep 1 arrow When the tip touches bottom of well enter the value shown in head position into the tip bottom value shown under save current position To determine if tip is at bottom of well try slightly moving plate by hand while moving head down Once you feel resistance then that means tip has reached bottom of well At the correct tip bottom position the plate can be moved side to side but not up or down 73 9 Using the arrow up key move the head pipette tip above the top surface of plate The bottom of the tip should be moved to a position above the plate that it has no chance of hitting any objects on the deck if the deck was to be moved This is the position that the tip will be at while the deck is moving the plate so that it will clear the plate This is considered the tip safe position Enter the Head position value into the save current position as tip safe value 10 Enter the volume of liquid in the wells of the well plate Actual Rack Magazine Land E Deck Pos zl I Orient IL f pelo enter To enter values in the save current position as click on the blue text next to position that requires changing
53. other screen will pop up asking Change the magazine and select the next tip row Next tip row Note Changing magazine is not required if you would like to continue to use same tips 12 S81 Plate to Plate SuULA A hs Description This parameter deter the bottom shape of PLATE 1 TYPE is used to indicate the design of the bottom of plate 1 This information is used to determine the height of the liquid in each well and thus calculate how far down into the wells the tips should go If you are not using a standard microplate select CUSTOM and determine the amount to raise or lower the tip height 23 Sea Plate to Plate SuL A Plate Type hs Description This parameter determines the bottom shape of the plate PLATE 2 TYPE is used to indicate the design of the bottom of plate 2 This information is used to determine the height of the liquid in each well and thus calculate how far down into the wells the tips should go If you are not using a standard microplate select CUSTOM and determine the amount to raise or lower the tip height LINK TO FILE is used to indicate what the system should do after it completes the file it is currently running If you want the system to stop at the end of the file do not enter anything in the link file field Plate to Plate Transfer eu M Enter Delay Time 8 60 9999 sec CEGET DEDOS 9T TT T 7 ais c e r e 1 t sw o ie a a s r v v u x v z eee Jos c
54. plate from a Micro Trough Reservoir The Plate Filling button is used to establish a user file which can be stored 12 56 07 Plate Filling Gull A Actual Ualues aran ls Volume in wel amp uL Deliver from eIL88rmL 1 Mixes in Trof 3 Trof mix volume 288uL Delivery vo lume e 166uL Mixes after transfer E 5 2 Creating and Editing a Plate Filling File When you touch the Plate Filling button the opening plate filling screen is presented You can change the value of each parameter to provide the desired program to fill the plates To change a plate filling parameter touch the text in blue and enter the desired value 20 12 56 55 Plate Filling cuLA A Actual Ualues Screen 143 Volume in uell amp B uL Deliver fram eTr88nL 1 ni Change actual values to default values e Mi me Next RR EB Helper F Press the Def Values button to change back to system default values Plate Filling ZuL A Actual volume in well gul ra 2888 bi Description J This parameter determines haw much liquid is in each well before any tranfer has made Touch the number to change woalue The value is an integer etween C and See uL 12 37 06 B Helper ACTUAL VOLUME IN WELL AT START is used to indicate how much liquid is in each well before any trans
55. position adjustment for non standard microplates in a custom file and manual moving head and deck positions Hain Menu Plate Ser ial Plate Filling Dilution To Plate Cherry Pick ing Sett ings 2 35 19 Pressing the Settings button will open the Settings screen 62 Settings Hanga e Files 14 11 11 Pressing the Set Clock button will open a screen where the settings can be changed Pressing the Set Serial Port will open the screen for changing the Serial Port configuration These settings will be set at the factory Monday 26 X February gar 14 11 41 Pressing the text in blue will allow you to change the variable date settings Press OK button after changing the variable to accept the change 63 Serial Port Conf igurat ion Baud Rate 9608 E Por ity None v 2 Data bits 8 Im El Stop bits 1 iw El Gy Press l v 1 to change the values The serial port configuration allows for setting values required to communicate to external computers or other devices The values are preset at the factory Manage User Files Doun load Al Files to PC Restore All Files from PC Delete ALL User Files Download Settings to PC Restore Settings from PC Status The Manage user files DownLoad Position Settings and Files from Computer to the WellPro e Connect serial cable to back of WellPro and to Computer e Confirm on Touch screen that settings are Bau
56. proper draw off of liquid from the tip depends heavily on its depth in the liquid that it is in contact with Tips not manufactured by ProGroup Instrument Corporation may decrease the accuracy and precision of the system 12 3 Power Interruptions If the power supplied to the WellPro has been interrupted the following actions will happen when the power is restored 1 If you were editing a file you will lose the file and will have to re edit the file 2 If you had just stored a file the WellPro will store the file 3 f you are in the middle of a run you will lose the run Homing the instrument will sequence it back to a point where you can start over 12 4 Maintenance 12 4 1 Preventive Maintenance To ensure trouble free operation keep the WellPro liquid handler clean and free of chemicals dirt and dust To ensure trouble free operation you should keep your WellPro liquid handler clean and free of chemicals dirt and dust Any liquids spilled on the WellPro liquid handler should be wiped off immediately 80 Keep your WellPro liquid handler free of any corrosive reagents Wipe spills immediately Clean non contaminated work surfaces regularly with a damp cloth 12 4 2 Cleaning up Spills If solution is accidentally spilled on the instrument clean them up immediately Although all electrical components within the instrument have been either shielded or sealed against fluid contact there may be long term deleterious effects
57. r Reagent Troughs Deck Position 1 96 or 384 well plate in Landscape Orientation This position can also accomodate portrait Fig 1 2 Standard Template for Long Bed WellPro showing plates positioned in landscape orientation Position 2 can accomodate well plates roughs deep well blocks and tube racks EE shown plates tube racks and deep well blocks Fig 1 3 Standard Template for Long Bed WellPro showing plates positioned in landscape orientation Fosition 2 Chilled rough with disposable liner far plate filling Fig 1 4 Standard Template for Long Bed WellPro showing plates positioned in landscape orientation Position 2 with 3 cavity rough shown s ml 50 ml 20 ml for plate filling Fig 1 5 Standard Template for Long Bed WellPro showing plates positioned in landscape orientation Position 1 with well plate in portrait orientation serial dilution In portrait Fig 1 6 Standard Template for Long Bed WellPro showing plates positioned in portrait orientation Albo lap aa alk i TEJ 5 T Fig 1 7 Standard Template for Long Bed WellPro showing plates positioned in portrait orientation 10 e Standard Templates for WellPro with a Short Bed The short bed requires two templates One template is for positioning plates in a landscape orientation and the other template for portrait orientation Landscape template can accept well plates orientated 96 well plates 12 well ro
58. r than the volume selected 13 30 06 Serial Di lution Transfer volume 56 uL 6 288 Lt Description This parameter determines Aow much liquid to withdraw from the original well Touch the number to change woalue The value is an integer etueen C and 2ee uL I BIDS The above display is for WellPro 12 channel 96 well plate 36 Serial Dilution SuL kK Transfer volume 26 ul 6 56 ES Description This parameter determines how much liquid to withdraw from the original wel Touch the number to change walue The value is an integer etueen c and ee uL 13 3202 15 I Fe ir The above display is for WellPro 24 channel 384 well plate TRANSFER VOLUME indicates how much sample should be withdrawn from the original well for transfer to the new well during a serial dilution This parameter can be set from 0 to 50 LL in 1 uL increments for 384 well plates and O to 200 LL in 1 uL increments for 96 well plates A transfer volume of 0 may be used if you wish to simply mix the contents of the well 13 30 42 Serial Di lution Nixes before transfer 3 8 9 ki Description You can speci fuy mixing of the well contents before an aliquot is transfered Touch the number to change wol ue The value is an integer hetween and SS zero indicates NO mixing MIXES BEFORE TRANSFER is used to indicate the number of aspiration delivery cycles that should be performed before transferring a sample As
59. ration To select row A press the A button which will then fill in with red If you want all rows to be filled press each button For rows that you don t want filled then do not press the button with corresponding row letter Non filled rows will have no red filled in on the box ENTER FIRST TIP ROW is used to specify which row of tips to use Rows of the tip magazine are designated by letters A through P back to front or A through H 42 First Tip Row NAT Setting the next First Tip Row to NAT will keep track of next available tip that has not been used This should be set at NAT if you are linking programs or wanting to keep track of next row of tips to be used while manual running programs If you want to reset the next available tip row to the beginning then at screen one press Rst NAT See below 13 43 53 Serial Di lution Actual Values Screen 343 Fill plate E P os a Soy Back When you press Rst NAT located on screen one a Pop Up screen will be displayed asking to make sure the deck is OK If you press OK another screen will pop up asking Change the magazine and select the next tip row Next tip row Note Changing magazine is not required if you would like to continue to use same tips 43 13 44 21 Serial Dilution ulf A Actual Ualuez Fill plate E D screen 3573 Hake sure the deck is set Press OK to run the appl ication Touching Pressing the GO button will pop up a message to confirm that the
60. rear Jenter T c ue ID E If you want the system to perform another file after the present file is complete enter the name of the file in the enter name field and enter amount of seconds would like for time delay before running the next file Press enter after entering text in the fields to accept and then exit Pause Enter Delay Time Instead of a time delay you also have the option to have a pop up screen prompt you to press continue before starting the next program This is done by entering the number 9999 which will create the pause before proceeding command 54 14 01 05 Plate to Plate Sul A Actual Values Screen del Irons er A B BE IILICICICICICICI a ERE eke Hele 1 to 1transfer Above display for WellPro 12 channel 96 well plate Plate to Plate Transfer PuL A Actual Values Screen del A B cC D Tronstor M ER rou ad Bee E Gack 2i eS i1 1 to 1transfer Above display for WellPro 24 channel 384 well plate TRANSFER ROW is used to indicate which row of the source TRANSFER plate is used to place liquid in during transfer to the corresponding row on destination plate 55 ETE Plate to Plate Actual Values Screen 4 4 esa A B D aooododa Destination E BE PL apodo soi Bec 1 to Several Above display for WellPro 24 channel 384 plate SOURCE ROW the SOURCE row specified acts as the single source row for transfer to several receiving or DE
61. rogram 45 Im Seri ial Di lut i ion oua x Enter Mame SD j2 3 4 s e v 8 9 e jel Ale ic ole lr ie ali ly JK L in m oj ejoa n s r uju u x v je AMES Beet eters lates GU 13 49 50 Help Saving a Program after setting the variables to desired value go back to screen 1 of the serial dilution menu and press the Save button to save the parameters as a program file Use the keyboard on the display to enter the alpha numeric name of the program and then press enter and save The save can be performed after setting the variables and before pressing GO to run the program Or the program can be saved after running the program by pressing the back button to screen 1 of the serial dilution menu and then press save button Serial D MA EN Select File SD a al Be Description llse the arrows to find the file Fress CLoqad to load the file Fress Cancel to exi 19 50 28 scr SEIEN To load a saved program press the load button and then select a file to load by using the up or down arrow scroll buttons Press the load button then go to next screen and press GO button to run program 46 7 PLATE TO PLATE TRANSFER 7 1 Overview Transferring an aliquot of sample from one plate to another is called plate to plate transfer or replica plating This technique allows for transfer of samples in a SOURCE plate to a RECEIVER plate for assay Culture etc The steps required to perform the plate transfer are
62. s an additional configuration option A 12 or 24 channel tip air displacement pipetting head is included on the WellPro liquid handler as most protocols are designed to be performed in the 12 or 24 channel mode but serial dilutions can be performed in the 8 or 16 channel mode to allow for maximum dilution capability with the 96 or 384 well plate The table assembly drives the plate which holds the well plates and the Micro Trough reagent containers The precision of this sensitive device is affected by a series of variables These include the degree to which the pipette tips are able to be wet which is dependent on the viscosity of the pipetted liquid the dimensional accuracy of the tip orifice piston speed and delay time Therefore please keep the following in mind when using the WellPro Liquid is pipetted with vacuum pressure generated by the pistons This technique requires dwell time to equalize air pressure in the pipet tips during pipetting Pipetting time is highly dependent upon the properties of the liquid factors It is necessary to allow some dwell time during pipetting operations particularly for smaller volumes and viscous liquids Changing the aspirate speed to a lower setting of medium or slow will provide more dwell time Rinsing if dry tips are used more time is required to saturate the dry air with moisture Vapor pressure increases above the pipetted liquid inside a dry tip As a result dry tips may cause liquid to be driv
63. s to find e to erase 9a ile to erase to exit Help To delete a program you can choose delete to delete specific files you have saved in the serial dilution plate to plate and filling sections Choose delete file to delete a specific file program A message will pop up Do you want to delete this file you can choose yes or no to continue 13 25 43 Plate Filling Gull A Actual Ualues Screen 1 3 Volume in uell amp B uL Deliver fram eTLr88nmnL 1 ni Change actual values Tre to default values De Hi Rst NAT Reset next available tip row Pressing this will reset the next available tip row to the first row in the tip rack Rst NAT is only active when NAT is selected on last screen in the First Tip Row field Rst VOL Will reset the volume in the trough This allows tracking the level of liquid in the trough as it is used This is only active if Tracking is set to ON A GO button exists at the lower right hand corner of every screen This can be pressed at anytime to run the current program loaded into the system 33 6 SERIAL DILUTION 6 1 Overview Serial Dilution is the progressive dilution and redilution of a liquid In this process a sample is added to a diluent and the two are mixed An aliquot is then removed for addition to the next diluent containing well This sequence is repeated until the desired number of dilutions have been obtained serial
64. t please contact your local ProGroup Technical Service Department A ProGroup Instrument Corporation technical service representative may be able to resolve the problem over the telephone using a basic check out procedures If the problem cannot be resolved in this manner the technical service representative will arrange for a field service representative to visit your lab to repair the unit If the unit cannot be repaired in your lab arrangements will be made to return the unit to ProGroup for factory repair Only qualified personnel should attempt service or repair work on the WellPro operating circuits or mechanism 81 Performance Specifications Pipetting Channels 24 way format Volume Range 1ul to 50ul in 1ul increments Precision A 100ul lt 1 0 CV 50pl Do f 096CV 10pl sul lt 3 0 CV 1l pod 5096 CV Accuracy AAN 100pl 50 OO 10l sl lt 2 0 ul BO Throughput mess Serial Dilution OOOO 50ul Transfer Volume asas No Mixing No Tip Change 25 seconds 40 seconds Landscape Format Plate to Plate Transfer AA 50ul Transfer Volume No Mixing With Tip 1 minute 25 seconds 2 minutes 50 seconds Change on each row Landscape Format Plate Filling p 100ul Transfer Volume MO MIRE NO 1110 Gene 50 seconds 1 minute 30 seconds Landscape Format Plate Formats 96 well standard shallow deep 384 well standard shallow well microtube V U and flat deep well V U and flat bottom bottom Functions S
65. tes the maximum TRANSFER VOLUME for the row by dividing the VOLUME IN WELL AT START by the number of transfers specified for that row 13 52 57 Plate to Plate Actual Values Screen 24 Nixes before transfer 6 Mixes after transfer e 8 nix Yo lume amp Liquid handling speed amp Change Tips _ gl Back Next mie MIXES BEFORE TRANSFER is used to indicate the number of aspiration delivery cycles that should be performed before transferring a sample For example when you are diluting cells which may settle you should agitate them back into suspension before transfer by mixing This parameter can be set from O to 9 MIXES AFTER TRANSFER is used to indicate the number of aspiration delivery cycles that should be performed after transferring a sample For example when you are diluting cells which may settle you should agitate them back into suspension after transfer by mixing This parameter can be set from 0 to 9 MIX VOLUME is used to tell the system how much solution should be used in the Mix step CHANGE TIPS is used to indicate if the tips should be removed after the first liquid is dispensed and a new set of tips from the tip magazine should be placed on the pipette head thus decreasing carryover If you don t want to change tips then the same tips will be used throughout the entire operation 21 Iss So Plate to Plate Sul A
66. the operation and maintenance of the ProGroup Instrument Corporation WellPro WP3000 Liquid Handler Read the manual in its entirety prior to operating the instrument This User Manual has been designed to give you the information you need to This User Manual also describes features and specifications of the ProGroup Instrument Corporation Review safety precautions Install the system Use the system in routine jobs and research Perform basic maintenance procedures WellPro WP3000 Liquid Handler This manual includes the following sections Installation System Components Plate Filling Serial Dilution Plate to Plate Transfer Position Settings Managing Files Linking Files Adjusting the Tip Position for Custom Well Plates Operating Hints and Maintenance Specifications 13 2 INSTALLATION PROCEDURES 2 1 Overview The ProGroup Instrument Corporation WellPro 3000 Liquid Handler is designed to be installed by the operator This chapter describes how the unit is to be unpacked and assembled 2 2 Location of the Unit in the Laboratory The unit should be installed in a facility with the following conditions e The temperature should be maintained between 20 26 C 68 79 F To prevent temperature fluctuations do not place the WellPro 3000 liquid handler in direct sunlight near equipment that gives off heat or under heating or air conditioning outlets e If flammable or toxic solvents are to be used a suitable ventila
67. the tips to the rack and abort the program 31 Plate Filling euLB X Enter Name PF1 Et Cancel Save 3T2T3 TS Te T T9 T T T T7 aja c e r e 1 x e w o aja s r v v u x Y z seee Def 191357 Ep H Saving a Program After setting the variables to desired value go back to screen 1 of the plate filling menu and press the Save button to save the parameters as a program file Use the display keypad to enter the desired program name The name can be any alpha numeric name After entering the name click on enter button and then the save button You are now ready to click on the exit button and go back to main plate filling screen The save can be performed after setting the variables and before pressing GO to run the program Or the program can be saved after running the program by pressing the back button to screen 1 of the plate filling menu and then press save button Plate Filling eu gx Select File PF1 aj Cance imu EE Description E llse the arrows to find the file Fress CLoagd to load the file Fress Cancel to exit 19 15 02 WHat Heip To load a saved program use the up or down arrow keys to scroll and select the program to load and run This will load the parameters for the program to be run Then go to the next screen and press GO button to run program 32 13 22 44 Plate Filling Gull A Select File Es Description se the arrow
68. tion system should be provided e The use of open flames in the laboratory should be prohibited e Corrosive vapors or dust should not be present as these materials can adversely affect the long term performance of the system The Well Pro 3000 module requires approximately 24 5 x 11 50 x 16 5 of lab bench space 14 lC Note Place the ProGroup Instrument Corporation WellPro 3000 on a stable level bench or hood Excessive jarring or vibration during operation may affect its performance 2 3 Power Requirements The ProGroup Instrument Corporation WellPro 3000 Liquid Handler is designed to operate at 100 120 220 or 240 V 50 60 Hz with a maximum power consumption of 360 watts The operating potential is set at the factory and is indicated on the serial number tag on the rear panel of the system WARNING WARNING Verify that this voltage is correct for your location before installation cAUTION CAUTION ProGroup Instrument Corporation WellPro 3000 Liquid Handlers shipped to locations in the United States and Canada are supplied with a standard three conductor power cable and a three terminal plug that provides an earth ground The unit MUST be connected to a three terminal socket that is properly wired according to the U S National Electrical Code and is grounded to a true earth ground The use of an adapter to a two terminal outlet is PROHIBITED 15 2 4 Unpacking and Inspection The ProGroup Instrument Corporation WellPro 3000
69. to Printer 67 wellpre Hyper Terminal File Edt View Call Transfer Help Send Text File Look in EJ Desktop O ge i my Documents E My Computer My Recent 8 My Network Places Documents 3 Desktop My Documents hy Computer e File name ve My Network Files of type Text file TXT e IMPORTANT WAIT FOR THE END COMMAND TO SHOW IN HYPERTERMINAL MENU AND LISTEN FOR A BEEP sound before disconnecting Now you can press call disconnect and close the Hyperterminal menu e For downloading the program files to Wellpro repeat the same process used for downloading the settings except for now click on Restore all files from PC in the manage user file section of touchscreen instead of Restore all files from PC 68 Upload Customer Settings and Files from WellPro to a Computer e Connect serial cable to back of WellPro and to Computer e Confirm on Touch screen that settings are Baud Rate 9600 Parity None Data Bits 8 Stop Bits 1 e Click on the Hypterminal Icon located on Desktop e Go back to Hyperterminal menu on computer and Transfer then Click on Capture Text and pick the location and file name you would like to save it as amp wellpro Hyper Terminal File Edit View Call BER O me sf Send File Receive File Capture Text Send Text File Capture to Printer 69 e wellpro HyperTerminal Capture T
70. transfer from row A of plate 1 to rows A and B of plate 2 then automatically continue with another file which transfers from row B of plate 1 to rows C and D of plate 2 and so on TRANSFER MODE DIRECTION also allows you to use a 384 well plate 24 channel head or 96 well plate 12 channel head as a reagent reservoir to deliver multiple reagents to a single assay plate You can place the same or different reagents in each of the 24 or 12 columns and or each of the 16 or 8 rows of the source plate These reagents can be delivered to specified rows on the destination plate This arrangement minimizes reagent waste TRANSFER DIRECTION is used to indicate whether the system should transfer from plate 2 to plate 1 or from plate 1 to plate 2 i e either plate 1 the plate in the front cavity of the template or plate 2 the plate in the middle cavity can be used as the source plate IEEE Plate to Plate SuL Plate 1 volume well uL 8 726808 EE Description E This parameter determines haw much liquid is in each well before anu tranfer has made Touch the number to change woalue The value is an integer etween and ze uL VOLUME IN WELL PLATE 1 is used to indicate how much liquid is in each well of plate 1 The system uses this value to determine how far down into the wells the tips should go and also to prevent exceeding the volume capacity of the wells 49 Sian ters Plate to Plate SuL A Plate 2 volume well 166 uL
71. two or more stored files as a sequence enabling the user to automate very complex liquid handling operations This prompt permits you to direct the WellPro liquid handler to transfer control to another file immediately after completing the file that it is currently running instead of terminating the operation of the file 78 Plate to Plate Transfer B uL Actual Values Screen 3 4 Plate 1 tupe FLAT Plate type FLAT Link files CEGET DEDOS 9T eT na ais c e r e w 1 x e m fof aja s r v v u x v z ses os Tere Jer 11 2 Using Linked Files When the system is operating using a linked file sequence the sequence will use the tips indicated in the ENTER FIRST TIP ROW prompt for the first file in the sequence This prompt appears regardless of whether the tips are to be changed in the first file The user should respond by specifying the row of tips that you want the WellPro liquid handler to select first The WellPro uses the same counter to keep track of which tips have been used whether you have specified tip changes or not Accordingly each time the WellPro liquid handler links to the next file in a chain it sequences through the tip magazine to select the next row of fresh uncontaminated tips Likewise if you start a new file or restart the same file the counter automatically increments causing the WellPro liquid handler to select the next row of unused tips When the last row of tips row A has been
72. ws A H or 384 well plates 24 well rows A P landscape as shown in Figure 1 8 The portrait template accepts plates in a portrait orientation 96 well plates 1 12 or 384 well plates 1 24 well columns as shown in Figure 1 9 1 La 7 z g EL S LE th PL a PF a F Fm TAE EATAZA TA RA RA ARA ROS orientation for plates ubes and troughs Position 1 for well plates and tube racks in landscape orientation Fig 1 8 Standard Landscape Template for Short Bed WellPro showing plates positioned in landscape note for portrait orientation this template must be removed and replaced with a portrait template 11 EID On ora cb dti 2 ie Es i T AS P F a mw RE Fortrait orientation for serial dilution This Is fo short bed vvellPro only emplate must be changed If landscape orientation Is required Fus Teo E EIE EXCESS USA HR mae he j a g div T1 b r T m Mote Plate filling in portrait orientation on he short bed WellPro can not be done If ling In portrait Is required then must use along bed vvellPro Fig 1 9 Standard Portrait Template for Short Bed WellPro showing plates positioned in Portrait note for landscape orientation this template must be removed and replaced with a landscape template 12 About the User Manual This User Manual has been written for the end user of the system e g a laboratory technician and provides information about
73. y Line delay E milliseconds Character delay milliseconds ASCII Receiving Backspace key sends CH Del C3 EtrH Space Ctrl H Emulatian las Terminal Setup Telnet terminal ID 427100 an Append line feeds to incoming line ends Force incoming data to 7 bit ASCII Wrap lines that esceed terminal width Backscroll buffer lines Flay sound when connecting or disconnecting Input Translation ASUII Setup 66 wellpro Properties Connect To settings Function arrow and ctrl keys act as Terminal keys CO Windows keys Host System Encoding Method fx Select Host System Encoding Method Backspace key sends ChitH Del 5 Shift JI5 C2 Standard JIS EtrH Space Ctrl H E mulation nsw Telnet terminal ID TI Dt 300 Backscrall buffer lines Play sound when connecting or disconnecting Input Translation ASCII Setup e Go to Touch Screen and into Manage Files e Click on Restore settings from PC e Go back to Hyperterminal menu on computer and Transfer then Click on Send Text File and pick the file to send from WellPro Backup folder on desktop amp wellpro HyperTerminal ence Help Send File Receive File Capture Text Send Text File Capture

User Manual - ProGroup Instrument Corp.

Contents

Download Pdf Manuals

Related Search

Related Contents