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Waters Inline Degasser AF User Manual

1. Front Panel Figure 5 7 Vacuum Tubing Schematic for a Degasser with Two Vacuum Chambers Control Board Rear Panel Front Panel Figure 5 8 Vacuum Tubing Schematic for a Degasser with Three Vacuum Chambers 71 Troubleshooting and Service Rear Panel Front Panel Figure 5 9 Vacuum Tubing Schematic for a Degasser with Four Vacuum Chambers Service 72 Appendix A Specifications This appendix lists the operational specifications of the In Line Degasser AF Table A 1 Operational Specifications Item Specification Chemical resistance Unaffected by full range of organic solvents and aqueous solutions of acids bases salts and surfactants pH range 0 to 14 Gas removal efficiency Varies with flow rate see Table 4 1 Typical operating flow range 200 uL min to 5 mL min Equilibration time lt 1 hour Pressure drop across one channel lt 0 08 psi 0 55 kPa at 1 mL min Milli Q water STP Wetted surfaces PPS Teflon AF Tefzel Inputs Enable Disable External control Output dc voltage Relative vacuum Eluent connections 1 4 28 rev
2. 6 Insert the other end of the tubing halfway into the TFE tubing 7 Slide the TFE tubing over the fitting of the draw off valve inlet on the pump 3 3 Installing with Non Waters Pumps This section describes how to connect the In Line Degasser AF to a non Waters pump Locate the In Line Degasser AF on the side of the pump closest to the eluent inlet of the pump The In Line Degasser AF is most effective when the fluid lines from the eluent reservoirs and to the pump are as short as possible Long sections of tubing allow ambient gases to dissolve back into the degassed eluents Installing with Non Waters Pumps 43 3 4 Install the In Line Degasser AF between the eluent reservoirs and the pump inlet Use the Tefzel tubing compression screws and ferrules in the Startup Kit to make connections to the In Line Degasser AF Use the fittings provided by the pump manufacturer to ensure leak free connections to the pump Installing the Vent Line In addition to removing dissolved gases from the eluents the In Line Degasser AF may remove some eluent components as vapor These vapors may condense to form droplets in the exhaust system The In Line Degasser AF exhausts these gases and droplets if any through a vent fitting on the rear panel as shown in Figure 3 9 This section describes how to safely vent these emissions LT Vent Fitting Figure 3 9 Vent Fitting on Rear Panel system such as a properly
3. The vent line is properly installed Powering Up To power up the In Line Degasser AF 1 Push the Power switch on the front panel to the On I position The LED on the front panel lights and the following events occur a The vacuum pump starts b The vacuum chambers pump down c The vacuum pump switches to low speed mode The vacuum pump begins operating after a few seconds and pumps down the vacuum chambers for approximately two minutes At the end of the pump down cycle the vacuum pump switches to low speed mode The In Line Degasser AF is ready for operation when the LED on the front panel is a steady green color Powering Up 46 4 2 If the vacuum is not within specification the LED on the front panel flashes on and off If this occurs refer to Chapter 5 Troubleshooting and Service to identify and correct the problem 2 Start the HPLC pump and begin your chromatography Note Start the In Line Degasser AF at least 2 5 minutes before you start to equilibrate your HPLC system This provides enough time for the In Line Degasser AF to reach maximum vacuum Running the In Line Degasser AF while you equilibrate the system minimizes baseline noise In Line Degasser AF Operation 4 2 1 47 This section describes Operating statuses of the In Line Degasser AF Degassing efficiency Operating Statuses The Waters In Line Degasser AF operates automatically while you perform your HPLC runs There are no control
4. Note To prevent eluent contamination use an ultra pure carrier UPC grade of helium The combination of inert gas nitrogen or helium sparging and inline degassing is an effective method of reducing dissolved oxygen to the lowest possible level See Section 1 2 2 Effects of Dissolved Oxygen for a discussion on the effects of dissolved oxygen A combination of helium not nitrogen sparging and inline degassing is best at flow rates above 5 mL min to maintain a minimum concentration of dissolved gases Note Degassing methods must be implemented carefully with eluents containing volatile components Chromatographic performance may be altered by minor changes in eluent composition Controlling the Degasser Externally This section describes how to control the Waters In Line Degasser AF from an external device Using the External Control terminals on the rear panel you can place the degasser in either of the following modes Enabled mode Disabled mode Enabled Mode An open circuit on the External Control terminals on the rear panel of the In Line Degasser AF places the degasser in the enabled mode This is the default setting when there are no connections to the terminals The following events occur when the In Line Degasser AF enters the enabled mode either from a power up sequence or from a switch open signal to the External Control terminals 1 The LED on the front panel turns on 2 The vacuum pump begins a pump
5. UL CSA 5 mm x 20 mm 230 187 to 264 1 6 A time delay IEC 5 mm x 20 mm The In Line Degasser AF is shipped with the correct fuses installed for 110 V operation If you plan to operate the degasser with 220 V power insert the proper fuses supplied in the Startup Kit into the fuse holder The fuse holder is located on the rear panel of the In Line Degasser AF Figure 2 1 Note Use the UL CSA rated fuse to meet North American agency standards and the IEC rated fuse to meet international agency standards Caution To avoid electric shock power down the In Line Degasser AF and unplug the power cord before you replace fuses Caution For continued protection against fire hazard replace fuses with those of the same type and rating 27 Making Electrical Connections 6 E B000 Fuse Holder Figure 2 1 Fuse Holder on Rear Panel Installing Fuses To install the fuses 1 Remove the power cord from the receptacle on the rear panel Caution To avoid electric shock power down the In Line Degasser AF and unplug the power cord before you replace the fuses 2 Using your fingers or a pair of needle nose pliers pinch in the two retaining clips on each side of the fuse cover Pull out the fuse holder Remove the incorrect or burned out fuses Refer to Table 2 1 to determine your fuse requirements Insert two new fuses into the fuse holder o a A o I
6. in each folder A right arrow between menu options indicates you should select each option in sequence For example Select File Exit means you should select File from the menu bar then select Exit from the File menu Related Adobe Acrobat Reader Documentation For detailed information about using the Adobe Acrobat Reader refer to the Adobe Acrobat Reader Online Guide This Online Guide covers procedures such as viewing navigating and printing electronic documentation from Adobe Acrobat Reader How To Use This Guide 13 Printing From This Electronic Document Adobe Acrobat Reader lets you easily print pages pages ranges or the entire electronic document by selecting Print from the File menu For optimum print quantity Waters recommends that you specify a Postscript printer driver for your printer Ideally use a printer that supports 600 dpi print resolution Conventions Used in This Guide This guide uses the following conventions to make text easier to understand Purple Text indicates user action For example Press 0 then press Enter for the remaining fields Italic text denotes new or important words and is also used for emphasis For example An instrument method tells the software how to acquire data Underlined Blue Color text indicates hypertext cross references to a specific chapter section subsection or sidehead Clicking this topic using the hand symbol automatically brings you to this topi
7. 18 Maximum external surface area exposed to the vacuum For greatest gas removal efficiency Maximum gas permeability For greatest gas removal efficiency Optimum chemical resistance For compatibility with a wide spectrum of liquids including all the mobile phases commonly encountered in HPLC The vacuum in the chamber accelerates the rate at which the dissolved gas diffuses through the polymer membrane into the vacuum chamber Section 1 2 Theory of Operation describes the principles of vacuum degassing The gases are exhausted through a vent on the rear panel As with all degassing methods some solvent vapor is also removed and vented along with the gas Section 1 5 Safety Summary describes the precautions necessary to minimize exposure to solvent vapors Vacuum System The vacuum system provides vacuum at a preset level to the connected vacuum chambers The vacuum system consists of the following elements Vacuum pump An electrical two speed stepper motor pump that creates a vacuum in the vacuum chambers Vacuum sensor A sensor on the control board that monitors the vacuum in the system The sensor signals the control board when the vacuum is below a preset level Vacuum chambers Contain the gas permeable eluent channel Gases are removed from the eluent in these chambers Each chamber is connected to the vacuum pump Electrical System The electrical system consists of the following comp
8. be valid Do not report this data until you ensure that chromatographic system performance is acceptable Table of Contents mi Mee p 12 Chapter 1 POTION MIT e Q 16 1 1 About the In Line Degasser AF eesseeseesesss 16 1 2 Theory of Operation PT 20 1 2 1 Operating Principles c2 RU ipo Et Uo REESE mde td 20 1 2 2 Effects of Dissolved Oxygen 21 1 2 3 Degassing Methods nne nete nnns 22 1 3 Installation Site Requirements ssssssssssss 23 1 4 Unpacking and Inspection eesesssssusssse 24 1 5 Safety Summary associer stops bd Ree re PIPE ER PEEI IS AE IA IM pMUS 25 Chapter 2 Making Electrical Connections scsscessspssccssedisnnssancaaareqcossesevsaaadenwnseoias 26 2 1 Voltage Selection and Fuse Installation 26 2 2 Connecting to Other Instruments ssssssssss 29 2 2 1 Connecting the External Control Terminals 30 2 2 2 Vacuum Terminals esuces esie ace dc DUREE ue dute ao sceesestecs 32 Chapter 3 Making Fluidic Connections E T uo 35 3 1 Installing with Waters 600 Series Pumps 37 3 2 Installing with Waters 510 515 or 1515 1525 APEG ule sees 40 Table of Contents 6 3 3 Installing with Non Waters Pumps ssssesss 43 3 4 Installing the Vent LIF
9. disabled 31 enabled 31 N Non Waters pumps 43 O Offline degassing 22 Online degassing 22 Operating principles 20 78 Operating status 47 55 Operational specifications 73 74 Outgassing 17 Outlet lines connecting 510 515 or 1515 1525 Pumps 42 connecting 600 Series Pumps 39 P Parts spare 75 Power connector on control board 63 Power requirements 26 Power supply 19 replacing 64 testing 62 63 Powering down 51 Powering up 46 Power up sequence 46 Pressure limit 36 Pumps 510 515 1515 1525 40 43 600 Series 37 39 non Waters 43 R Replacing control board 65 power supply 64 vacuum chamber 69 vacuum pump 67 Required materials fluidic connections 35 Required tools servicing 57 Requirements fuse 27 installation site 23 power 26 S Safety summary 25 Servicing required tools 57 Signal levels 32 Spare parts 75 Sparging 23 50 Specifications operational 73 74 Startup Kit 24 Status LED 47 52 operating 47 52 T Terminals description 30 External Control 30 vacuum 32 Testing control board assembly 65 power supply 62 vacuum chambers 68 vacuum pump 66 vacuum sensor 61 Theory of operation 20 Troubleshooting electrical 55 56 vacuum 53 55 Troubleshooting trees electrical 56 vacuum 54 55 79 U Unpacking the degasser 24 UV Vis detectors 21 V Vacuum chambers 19 additional 17 draining 60 61 installing in series 49 purging 58 replacing 69 70 testing 68 69 Vacuum pump 19 replacin
10. disconnected before proceeding 2 Remove the eluent lines from the front panel of the In Line Degasser AF as described in Section 5 2 2 Disconnecting Eluent Lines 59 Troubleshooting and Service N o 0 A co Remove the cover as described in Section 5 2 1 Removing and Installing the Cover Locate a section of vacuum tubing that contains no eluent Disconnect the end of the tubing at the connection farthest from the eluent Insert a syringe into the tubing and draw out the eluent Cut off 1 4 to 1 2 inch from the free end of each tubing or replace the vacuum tubing This ensures airtight connections Reconnect the vacuum tubing 9 Reinstall the cover as described in Section 5 2 1 Removing and Installing the Cover Draining the Vacuum Chambers To drain a vacuum chamber te AN 10 11 Power down the In Line Degasser AF and remove the power cord from the receptacle on the rear panel Caution To avoid possible electric shock ensure that the degasser is powered down and the power cord is disconnected before proceeding Remove the eluent lines from the front panel of the In Line Degasser AF as described in Section 5 2 2 Disconnecting Eluent Lines Remove the cover as described in Section 5 2 1 Removing and Installing the Cover Drain the eluent in the eluent tubing as described in Section 5 2 3 Purging the Eluent Tubing in Vacuum Chambers Drain any eluent in the vacuum
11. performance of refractive index detectors reducing baseline drift and irregularity Degassing Methods Methods for degassing may be performed Offline Online Inline Note Degassing methods must be implemented carefully with eluents containing volatile components Chromatographic performance may be altered by minor changes in eluent composition Offline Degassing Offline degassing involves procedures that you perform away from the HPLC system Some common offline degassing methods are Sonication with vacuum assistance Vacuum filtration Boiling Offline methods do not maintain the degassed condition Net diffusion of gases back into the eluent begins immediately after you stop the degassing procedure Within one to four hours the eluent is again saturated with ambient gases Online Degassing Online methods of degassing involve procedures that you perform at the eluent reservoirs during a chromatographic run The most commonly used method of online degassing is sparging 2 Williams D D and R R Miller Anal Chem May 1962 34 6 3 Bakalyar S R M B T Bradley R Hoganen J Chromatogr 1978 158 277 Theory of Operation 22 Sparging consists of bubbling an inert less soluble gas through the eluent reservoir before and during a chromatographic run Sparging although considered to be a degassing method does not degas the eluent Sparging replaces the air in solution with a lower concentratio
12. the Off 0 position The following events occur when you power down the degasser 1 The vacuum pump turns off 2 The LED on the front panel turns off Using the In Line Degasser AF Chapter 5 Troubleshooting and Service This chapter describes how to troubleshoot and service problems that may occur with the In Line Degasser AF The chapter consists of two sections Troubleshooting including troubleshooting trees Service instructions to use with the troubleshooting trees Note Under normal operating conditions the In Line Degasser AF requires no routine maintenance 5 1 Troubleshooting This section provides information for troubleshooting the In Line Degasser AF It covers Vacuum problems Electrical problems Overview Use the troubleshooting trees in this section to identify and locate problems within the In Line Degasser AF The degasser is designed to be serviceable by you Refer to Section 5 2 Service for descriptions of the procedures indicated in the troubleshooting trees safely glasses and gloves Know the chemical and physical properties of the eluents you 1 Caution Always observe safe laboratory practices when you are troubleshooting Wear are using Refer to the Material Safety Data Sheet for each eluent in use LED Indications The LED on the front panel indicates the status of the In Line Degasser AF as described in Table 5 1 Troubleshooting 52 Table 5 1 LED Indications LED S
13. 3 2 1 2 2 2 3 2 4 3 1 3 2 3 3 3 4 3 5 3 6 3 7 3 9 4 1 5 1 5 2 5 3 5 4 5 5 5 6 Waters In Line Degasser AF Two Channel 16 Major Systems in the In Line Degasser AF 18 Vacuum Chamber Schematic rti eiie e epe penus 20 Fuse Holder on Rear Panel sse 28 Hear Panel Connector aucexsenkFuximipdusaiedu uiua Edd R Xi Eo pEK Nx EX HEC QUKe 29 Waters 600 Series Controller Connection sss 32 Comparison of Vacuum Terminal Signal Output 33 Ferrule and Compression Screw Assembly 36 Positioning the In Line Degasser AF in a Waters HPLC System 37 Connecting an Inlet Line to the In Line Degasser AF 38 Connecting an Outlet Line to the In Line Degasser AF 39 Positioning the In Line Degasser AF with the Waters 510 or 515 Pump 1515 SIOWI u uc rtisoartun pn nsn drHdasa asa en bUb cc sw lu aC e ud 40 Positioning the In Line Degasser AF with the Waters 1515 or 1525 PUMP eee anne ere ee ena nan re een e nnn ee eee ree ee een 41 Connecting an Inlet Line to the In Line Degasser AF 42 Connecting an Outlet Line to the In Line Degasser AF 43 Vent Fitting on Rear Panel cceeeeeeeeeneeeeeeeeeeeeeeeneeeeeeeeeeenaaees 44 Two Vacuum Chambers in Series cccccccceeeeeeee
14. 4 15 16 Install the four M3 x 6 TORX screws and one star washer that secure the power supply board to the housing Reinstall the two connectors to the power supply board 12 Reinstall the housing cover Install the three M3 x 6 TORX screws securing the cover Reinstall the power supply housing into the chassis Install the two M3 x 6 TORX screws on the bottom panel and the M3 x 6 TORX screw on the rear panel securing the housing to the chassis Reinstall the power connector on the control board Reinstall the cover as described in Section 5 2 1 Removing and Installing the Cover Insert the power cord into the receptacle on the rear panel Service 64 5 2 7 Testing the Control Board Assembly Test the control board assembly after you have determined that the power supply is operating properly To verify that the control board is working correctly you need to check e LED Vacuum pump To test the control board 1 Power up the In Line Degasser AF 2 Check that the LED on the front panel lights up 3 Check that the vacuum pump turns on after a few seconds If the LED lights up and the vacuum pump turns on the control board is functioning properly If the LED does not light up or the vacuum pump does not turn on the control board is faulty Replace the control board as described next in Replacing the Control Board Replacing the Control Board Caution To avoid possible electric shock do not ope
15. AT073630 Control Board 0 700001351 Vacuum Pump 0 700001352 LED Assembly Multi Colored 0 700001353 Power Supply 0 WAT034507 Fuse Drawer 0 WAT079711 Compression Screw 1 8 inch package of 5 1 WAT037372 Ferrule 1 8 inch yellow package of 5 1 WAT037373 Tubing Tygon 2 feet 0 WAT079794 Tubing Tygon 0 125 in x 0 25 in 50 feet 1 WAT079731 Terminal Connector 6 Pin 0 WAT057241 Fuse 1 6 A UL CSA time delay package of 5 1 WAT079713 Fuse 1 6 A IEC time delay package of 5 1 WAT079716 75 Table B 1 Spare Parts Continued Recommended Item Stock Part Number Waters In Line Degasser AF Operator s Guide 0 71500030802 Startup Kit 0 WAT079718 76 Index C Connecting external control terminals 30 inlet lines 510 515 and 1515 1525 Pumps 41 inlet lines 600 Series Pumps 37 outlet lines 510 515 and 1515 1525 Pumps 42 outlet lines 600 Series Pumps 39 Waters 600 Series Controller 31 Connections making electrical 29 making fluidic 36 Connector power on control board 63 rear panel 29 Contacting Waters Technical Service 24 53 Control board 19 65 66 Control board assembly replacing 65 66 testing 65 65 Cover removing and installing 57 D Damage reporting 24 53 Degasser efficiency 23 48 inspecting 24 major systems 18 spare parts 75 specifications 73 unpacking 24 Degassing benefits 17 inline methods 23 offline methods 22 online methods 22 Detectors electrochemical 22 fluore
16. Caution hot surface or high temperature In Line Degasser AF Information Intended Use The Waters In Line Degasser AF can be used for in vitro diagnostic testing to analyze many compounds including diagnostic indicators and therapeutically monitored compounds When you develop methods follow the Protocol for the Adoption of Analytical Methods in the Clinical Chemistry Laboratory American Journal of Medical Technology 44 1 pages 30 37 1978 This protocol covers good operating procedures and techniques necessary to validate system and method performance Biological Hazard When you analyze physiological fluids take all necessary precautions and treat all specimens as potentially infectious Precautions are outlined in CDC Guidelines on Specimen Handling CDC NIH Manual 1984 Calibration Follow acceptable methods of calibration with pure standards to calibrate methods Use a minimum of five standards to generate a standard curve The concentration range should cover the entire range of quality control samples typical specimens and atypical specimens Quality Control Routinely run three quality control samples Quality control samples should represent subnormal normal and above normal levels of a compound Ensure that quality control sample results are within an acceptable range and evaluate precision from day to day and run to run Data collected when quality control samples are out of range may not
17. I oec Detto cove tto Dod e EERu 44 Chapter 4 Using the In Line Degasser AF auscasusssiesitdccdni nd baapiPi niti Ro aiias 46 41 Powering UD e a ste 46 4 2 In Line Degasser AF Operation ssseussuussse 47 4 2 1 Operating Statuses cocconsicainrcreansantanamieraaadadowmnnanannd 47 4 2 2 Degassing Efficiency iscritte reete recent 48 4 3 Controlling the Degasser Externally 50 4 4 Powering DOWN t 51 Chapter 5 Troubleshooting and Service use ues trier ea esisviilae 52 5 1 Troubleshooting seis rc 52 BSNS prr CC 57 5 2 1 Removing and Installing the Cover 57 5 2 2 Disconnecting Eluent Lines 58 5 2 3 Purging the Eluent Tubing in Vacuum Chambers 58 5 2 4 Draining the Vacuum Tubing and Chambers 59 5 2 5 Testing the Vacuum Sensor sssssss 61 5 2 6 Testing the Power Supply 62 5 2 7 Testing the Control Board Assembly 65 5 2 8 Testing the Vacuum PUMP ccr it tii nee eene 66 5 2 9 Testing the Vacuum Chambers 68 5 2 10 Replacing a Vacuum Chamber 70 Table of Contents 7 Appendix A cena pom IM eevee ewan enn yori a Appendix B Spare Parts Index Table of Contents 8 List of Figures 1 1 1 2 1
18. Waters In Line Degasser AF Operator s Guide Waters 34 Maple Street Milford MA 01757 71500030802 Revision A NOTICE The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation Waters Corporation assumes no responsibility for any errors that may appear in this document This manual is believed to be complete and accurate at the time of publication In no event shall Waters Corporation be liable for incidental or consequential damages in connection with or arising from the use of this manual 2001 WATERS CORPORATION PRINTED IN THE UNITED STATES OF AMERICA ALL RIGHTS RESERVED THIS BOOK OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER Waters is a registered trademark of Waters Corporation Kalrez Teflon and Tefzel are registered trademarks of E du Pont de Nemours and Company Milli Q is a trademark of Millipore Corporation TORX is a trademark of Camcar Division of Textron Inc Tygon is a registered trademark of Norton Company All other trademarks or registered trademarks are the sole property of their respective owners Q UALITY MANAGEMENT The Installation Category Overvoltage Category for this instrument is Level Il The Level Il category pertains to equipment that receives its electrical power from a local level such as an elect
19. Waters recommends that you place the In Line Degasser AF on the side of the pump that is closest to the pump inlet 1 4 Unpacking and Inspection The Waters In Line Degasser AF is packed and shipped in one carton that contains the following items In Line Degasser AF Unit Startup Kit Validation certificate e Waters In Line Degasser AF Operator s Guide Unpacking To unpack the In Line Degasser AF 1 Unpack the contents of the shipping carton 2 Check the contents of the Startup Kit against the Startup Kit List enclosed with the kit 3 Save the shipping carton for future transport or shipment Inspection If you see any damage or discrepancy when you inspect the contents of the carton immediately contact the shipping agent and your local Waters representative You can contact Waters Technical Service at 800 252 4752 U S and Canadian customers only Other customers call your local Waters subsidiary or your local Waters Technical Service representative or call Waters corporate headquarters for assistance at 508 478 2000 U S If any items are damaged use the shipping container for subsequent claim purposes Note Make sure the serial number on the nameplate located on the side panel matches the number on the validation certificate Unpacking and Inspection 24 1 5 Safety Summary The In Line Degasser AF vents the gases it removes through an outlet on the rear panel These gases may contain vapors of
20. c within the electronic document Right clicking and selecting Go Back from the popup context menu brings you back to the originating topic For example Section 4 2 2 Degassing Efficiency discusses the effect of different flow rates on the concentration of remaining gas Notes Attentions and Cautions Notes call out information that is helpful to the operator For example Note Record your result before you proceed to the next step Attentions provide information about preventing possible damage to the system or equipment For example STOP Attention To avoid damaging the equipment do not touch the flow cell window Cautions provide information essential to the safety of the operator For example Caution To avoid possible burns turn off the lamp at least 30 minutes before removing it for replacement or adjustment How To Use This Guide 14 instrument and unplug the power cord before performing maintenance f Caution To avoid possible electrical shock and injury always turn off the procedures Caution To avoid chemical or electrical hazards always observe safe laboratory practices when operating the system How To Use This Guide 15 Chapter 1 Introduction This chapter introduces the Waters In Line Degasser AF The following topics are covered Description of the In Line Degasser AF Theory of vacuum degassing Installation site requirements Unpacking instructions Safety consideration
21. connections to the screw type barrier terminal strips In addition ensure you always connect the shield of the cable to chassis ground at one instrument only You can control the operation of the In Line Degasser AF through the External Control terminals on the rear panel An open circuit between the External Control terminals enables the operation of the degasser A switch closure signal applied to the External Control terminals disables the operation of the degasser If you do not make connections to the External Control terminals the In Line Degasser AF remains in the enabled state when it is powered up Connecting to Other Instruments 30 Enabled Mode When you enable the In Line Degasser AF when it is powered up the power up sequence begins as described in Section 4 1 Powering Up The sequence includes an initial vacuum pump down cycle followed by continuous operation at low RPM to maintain the vacuum Disabled Mode When you disable the In Line Degasser AF the vacuum pump stops The In Line Degasser AF remains disabled until an open circuit signal is applied to the terminals You can send enable and disable signals to the In Line Degasser AF from a Waters 600 Series Controller that is connected to the In Line Degasser AF Connecting a Waters 600 Series Controller To connect a Waters 600 Series Controller to the External Control terminals 1 Use a signal cable included in the Startup Kit to make the connection
22. creases smoothly and stabilizes the vacuum pump is in good condition Go to step 8 If the voltage signal is unstable or remains at 0 5 V the vacuum pump is faulty Replace the motor pump assembly as described in Replacing the Vacuum Pump Reconnect the tubing to the original configuration Before reconnecting cut off 1 4 to 1 2 inch from the end of each tubing or replace the vacuum tubing This ensures airtight connections Reinstall the cover as described in Section 5 2 1 Removing and Installing the Cover Insert the power cord into the receptacle on the rear panel Replacing the Vacuum Pump power supply does not contain user serviceable components f Caution To avoid possible electric shock do not open the power supply cover The If the diagnostic flowchart indicates that the vacuum pump is faulty use the following procedure to replace the pump 67 Troubleshooting and Service 1 Power down the In Line Degasser AF and remove the power cord from the receptacle on the rear panel Caution To avoid possible electric shock ensure that the degasser is powered down and the power cord is disconnected before proceeding 2 Remove the vacuum tubing from the old pump 3 Remove the pump connector from the control board 4 Remove the three M4 x 16 screws securing the pump to the chassis Remove the pump 5 Install the new pump securing it to the chassis with the three M4 x 16 screws 6 Install the new pum
23. cuum terminals to Monitor the vacuum with a voltmeter Troubleshoot the In Line Degasser AF Signal Levels The Vacuum terminals deliver a 0 5 Vdc signal when the vacuum is at atmosphere For every decrease of 1 inch of mercury the voltage increases by approximately 0 759 Vdc The maximum voltage on the Vacuum terminals is approximately 2 7 V 0 2 V at maximum vacuum Connecting to Other Instruments 32 Note The In Line Degasser AF uses an absolute type pressure sensor Earlier versions of the In Line Degasser used a relative type pressure sensor The absolute and relative sensor types produce different signal readings for the same pressure value Figure 2 4 compares the vacuum terminal signal output of the In Line Degasser AF to the In Line Degasser internal mechanism with that of the In Line Degasser AF If your degasser has been updated in this manner the vacuum terminal signal output will reflect the absolute type pressure sensor of the In Line Degasser AF STOP Attention In some cases the In Line Degasser is updated by replacing the entire 3 5 3 0 2 5 Key In Line Degasser AF 2 0 Signal V In Line Degasser 0 5 10 15 psi Absolute 0 4 8 12 16 20 24 28 32 Inches Hg Pressure Figure 2 4 Comparison of Vacuum Terminal Signal Output 33 Making Electrical Connections Procedure To connect a voltmeter to the Vacuum terminals 1 Use a signal cable or the voltmeter leads to make the co
24. down cycle 3 Normal operation begins when the pump down cycle is complete Controlling the Degasser Externally 50 Disabled Mode A closed circuit on the External Control terminals on the rear panel of the In Line Degasser AF places the degasser in the disabled mode The following events occur when the In Line Degasser AF enters the disabled mode from a switch close signal 1 The vacuum pump turns off 2 The LED on the front panel flashes yellow 0 5 seconds on and 2 seconds off The In Line Degasser AF remains in the disabled mode until a switch open signal occurs at the External Control terminals External Control 4 4 You can externally control when the In Line Degasser AF turns on and off using a switch open and switch close signal from a Waters 600 Series Controller or any other device with contact closure outputs You can program a Waters 600 Series Controller to generate a switch open or close signal at a specific time This signal when sent to the External Control terminals on the In Line Degasser AF automatically enables the degasser to begin the pump down cycle before the start of arun The In Line Degasser AF is then ready to provide degassed eluent when a run is initiated Refer to Section 2 2 1 Connecting the External Control Terminals for the procedure to connect a Waters 600 Series Controller to the In Line Degasser AF Powering Down 51 To power down the In Line Degasser AF push the Power switch to
25. eeeeeeeeeeeeeeseeeees 49 Vacuum Troubleshooting Tree eeeeeeeeeeeee 54 Electrical Troubleshooting Tree esseeesssssssss 56 Testing the Vacuum Sensor isi sieucuicsicusateaswaatannkinaunasciunceveinunionsneudtbune 61 Power Connector Receptacle on Control Board Assembly 63 Vacuum Connections to Test the Vacuum Pump 67 Vacuum Connections to Test a Vacuum Chamber 69 Table of Contents 9 5 7 5 8 5 9 Vacuum Tubing Schematic for a Degasser with Two Vacuum Ghatmmbels saos o Hp s Hn M PME Vacuum Tubing Schematic for a Degasser with Three Vacuum Champers creates ce deste bet ul meade este dme re pies bue duse seas essct ases e edi Vacuum Tubing Schematic for a Degasser with Four Vacuum ODaltiDBES sca ee Rn bibet eR a a ECO Oe cin nicae Cn nue Table of Contents 10 List of Tables 2 1 2 2 4 1 Installation Site Requirements sessseeeeeees 23 Voltage and Fuse Selection nonet rn ute e tetas 27 Hear Panel Terminals iore ei ete accen tbe d or tb tad ED a dap ERDAV 30 Effect of Flow Rate on Final Dissolved Gas Concentration 48 Mz Mec ETT ooo TT 53 Tubing Schematics 5 ii aihcc setsleen ceviectnclageadsacenateaiageiGanceteennupasdunatiene 70 Operational Specifications eeecececeeeeeeeeeeneeeeeeeeeeeceeeeeeeeeeeeeaaees 73 Spare PA TS 35 pu Hi Du Or d ERST E PL PUR i e
26. egasser AF does not light when you power up the degasser The electrical troubleshooting tree helps you identify electrical problems in the following components Power supply Control board Vacuum pump 55 Troubleshooting and Service LED Off Power Up Degasser Insert Power Cord Replace Fuses Power at Wall Outlet Use Good Wall Outlet Degasser Yes Disabled Enable Degasser Replace LED Go to Vacuum Troubleshooting Tree Figure 5 1 Test Power Supply Replace Power Supply Test Control Board Control Board Replace Control Good Board Test Pump Electrically Replace Pump Test Vacuum Sensor Sensor No Good Yes Go to Vacuum Troubleshooting Tree Figure 5 1 Figure 5 2 Electrical Troubleshooting Tree Replace Control PCB Assembly Troubleshooting 56 5 2 Service The Waters In Line Degasser AF is designed to be serviced by you the customer The troubleshooting trees in Section 5 1 Troubleshooting describe the sequence of procedures to follow to identify a malfunctioning component This section describes how to perform the procedures indicated in the troubleshooting tree Required Tools Use the following tools to service the In Line Degasse
27. eries Controller which can be used to control the In Line Degasser AF Waters Licenses Warranties and Support Provides software license and warranty information describes training and extended support and tells how Waters handles shipments damages claims and returns How To Use This Guide 12 Documentation Conventions The following conventions may be used in this guide Convention Usage Bold Italic Courier Courier Bold Keys Bold indicates user action such as keys to press menu selections and commands For example Click Next to go to the next page Italic indicates information that you supply such as variables It also indicates emphasis and document titles For example Replace file_name with the actual name of your file Courier indicates examples of source code and system output For example The SVRMGR gt prompt appears Courier bold indicates characters that you type or keys you press in examples of source code For example At the LSNRCTL gt prompt enter set password oracle to access Oracle The word key refers to a computer key on the keypad or keyboard Screen keys refer to the keys on the instrument located immediately below the screen For example The A B screen key on the 2414 Detector displays the select channel Three periods indicate that more of the same type of item can optionally follow For example You can store filename1 filename2
28. ersed ferrule fittings 4 inlet and 4 outlet fittings Vacuum chambers One chamber eluent two channels standard third or fourth channel optional 0 5 mL internal volume channel Tubular membrane 1 vacuum chamber Teflon AF Vapor exhaust 1 8 inch 3 mm barbed fitting 73 Table A 1 Operational Specifications Continued Item Specification Vacuum source Built in 2 head diaphragm pump solvent resistant Vacuum sensor Detects vacuum from 0 to 15 6 psiA Maximum pressure on inlet and outlets 10 psi 70 kPa Dimensions Width 5 25 inches 13 33 cm Height 5 75 inches 14 60 cm Depth 12 0 inches 30 48 cm Weight 7 5 pounds 3 4 kg Power requirement 115 Vac 85 to 132 V 50 60 Hz 230 Vac 187 to 264 V 50 60 Hz Autosensing Ambient temperature 4 to 40 39 to 104 F Relative humidity 10 to 90 noncondensing High Density Polyethylene Polytetrafluoroethylene Ethylenetetrafluoroethylene 74 Appendix B Spare Parts The following spare parts are recommended for customer installation Use the number in the Recommended Stock column as a guide to the quantity of each item to keep on hand Table B 1 Spare Parts Item Rerarmmended Part Number Stock Vacuum Chamber 0 700001218 Vacuum Manifold Tube Assembly with Sipper 0 700001333 Vacuum Tube Replacement Kit 0 W
29. functioning fume hood T Caution To avoid contact with eluent gases connect the outlet vent to a suitable exhaust 44 To install the gas vapor vent 1 Use a length of 1 8 inch id thick wall tubing long enough to reach from the rear of the In Line Degasser AF to an appropriate exhaust system The Startup Kit contains a 15 foot 4 5 m length of FEP lined PVC tubing for this purpose If you need additional tubing see Appendix B Spare Parts for the Waters part number Making Fluidic Connections 2 Attach one end of the tubing to the barbed vent fitting on the rear panel 3 Place the other end of the tubing in a waste container in the venting outlet This container catches any incidental leaks or condensates from the In Line Degasser AF 1 Fluoroethylene propylene copolymer 2 Polyvinyl chloride Installing the Vent Line 45 Chapter 4 Using the In Line Degasser AF This chapter describes how to use the Waters In Line Degasser AF after it has been successfully installed The topics covered in this chapter describe Powering up the In Line Degasser AF Degasser operation Controlling the In Line Degasser AF from an external source Powering down the In Line Degasser AF 4 1 Powering Up Before Powering Up Before you power up the In Line Degasser AF make sure that Correct fuses are installed The power cable is connected Eluent tubing is correctly connected to the eluent reservoirs and the pump s
30. g 67 68 vacuum test 66 67 Vacuum sensor 19 testing 61 62 Vacuum system 19 Vacuum terminals 32 signal levels 32 Vacuum troubleshooting 53 Vacuum tubing draining 59 60 schematics 70 72 Validation certificate 24 Vent line installation 44 45 Venting 25 Voltage selection 26 Voltmeter connections 34 W Waters 510 515 1515 1525 Pumps 40 43 Waters 600 Series Pumps electrical connection 31 32 fluidic connections 37 39 Waters Technical Service contacting 24 53 80
31. h as methanol or tetrahydrofuran THF These complexes increase the background absorbance especially at lower wavelengths This leads to a small decrease in sensitivity of detection but more importantly to baseline shifts or ghost peaks during gradient separations Also a change in the dissolved oxygen level over time especially from reabsorption of ambient gases after using an offline degassing technique results in baseline drift and irregularity Removing dissolved oxygen to a reproducible level greatly enhances the performance of UV Vis detectors especially below 254 nm and in gradient systems This also improves sensitivity in certain fluorescence detection applications Effects on Fluorescence Detectors Oxygen may cause quenching of fluorescence response under certain mobile phase conditions with certain analytes at certain wavelengths Aromatic hydrocarbons aliphatic aldehydes and ketones are particularly susceptible to quenching Response decreases of 95 have been observed in some cases 1 Rollie Mae E Gabor Patonay Isaiah M Warner Ind Eng Chem Res 1987 26 1 6 21 Introduction Effects on Electrochemical Detectors Oxygen may interfere with various electrochemical detection techniques particularly reductive electrochemistry Effects on Refractive Index Detectors 1 2 3 Refractive index detectors are sensitive to changes in solvent density Removing dissolved gases to a consistent level enhances the
32. he dissolved gases diffuse through the polymer membrane into the vacuum chamber The gases are then carried away by the vacuum pump Figure 1 3 is a simplified schematic diagram of the vacuum chamber More Gas Gas Out Less Gas in Solution j to vacuum pump in Solution Eluent In gt L LR O Degassed XO g i e gt Eluent Out Eluent Channel Vacuum Chamber Figure 1 3 Vacuum Chamber Schematic The longer the eluent is exposed to the vacuum the more dissolved gases are removed Two factors affect the amount of time the eluent is exposed to the vacuum Flow rate Surface area of degassing membrane Theory of Operation 20 Flow Rate A lower flow rate increases the amount of time the eluent is exposed to the vacuum Section 4 2 2 Degassing Efficiency discusses the effect of different flow rates on the concentration of remaining gas Surface Area of Membrane The length of the degassing membrane is fixed in each vacuum chamber To increase the length of membrane you can connect two or more vacuum chambers in series This procedure is described in Section 4 2 2 Degassing Efficiency 1 2 2 Effects of Dissolved Oxygen Dissolved oxygen in the mobile phase may be of special concern as it can interfere under certain circumstances with the detection of analytes by UV Vis fluorescence or electrochemical detectors Effects on UV Vis Detectors Oxygen can form UV absorbing complexes with certain solvents suc
33. hown in Figure 3 3 Figure 3 3 Connecting an Inlet Line to the In Line Degasser AF 5 Install a filter on the end of the tubing 6 Mark the inlet line with a line marker A or B included in the Startup Kit to identify the reservoir supplying the eluent 7 Repeat steps 1 through 6 for each eluent line 38 Making Fluidic Connections Connecting Outlet Lines To connect an eluent line from the In Line Degasser AF to the gradient proportioning valve GPV 1 Drain any eluent from the eluent line that is connected to the GPV 2 Using the appropriate tool cut the eluent line on the pump to a length of approximately 18 inches 45 cm or long enough to reach the In Line Degasser AF 3 Install a compression screw and ferrule included in the Startup Kit to the free end of this line as described in the Assembling Fittings discussion at the beginning of this chapter 4 Connect the free end of the eluent line to the channel outlet on the front panel of the In Line Degasser AF that corresponds to the inlet line of the eluent Figure 3 4 To Pump Figure 3 4 Connecting an Outlet Line to the In Line Degasser AF 5 Mark the line with a line marker A or B included in the Startup Kit to identify the reservoir supplying the eluent 6 Check that the compression screw on the GPV is tight 7 Repeat steps 1 through 6 for each eluent line Installing with Wate
34. ith reduced drift and pressure fluctuations Reduced detector baseline noise for improved signal to noise ratio and more reliable quantitation Introduction Major Systems Figure 1 2 shows the three major systems within the degasser Eluent system Vacuum system Electrical and control system Key Eluent Lines Electrical and Control System Vacuum Chambers 4 Vacuum Lines Electrical Lines De oes Eluent IU um CORRI a Front Panel Rear Panel Vacuum System Figure 1 2 Major Systems in the In Line Degasser AF Eluent System The In Line Degasser AF removes dissolved gases from the eluent as it passes through a tubular membrane The membrane enclosed in a vacuum chamber is in the eluent flow path between the reservoir and the pump inlet The eluent enters and exits through inlet and outlet fittings on the vacuum chamber These fittings are labeled on and accessible from the front panel of the In Line Degasser AF Each tubular membrane is made of a proprietary specially engineered fluorocarbon polymer The membrane is designed for Minimum internal volume For rapid solvent changeover Minimum resistance to flow or pressure drop For ease in priming and accommodation of high flow rates About the In Line Degasser AF
35. ling Fittings discussion at the beginning of this chapter 4 Connect this end of the tubing to the inlet of an available channel on the front panel of the degasser Figure 3 7 Installing with Waters 510 515 or 1515 1525 HPLC Pumps 41 Figure 3 7 Connecting an Inlet Line to the In Line Degasser AF 5 Mark the line with a marker A or B included in the Startup Kit to identify the reservoir supplying the eluent Connecting Outlet Lines To connect an eluent line from the In Line Degasser AF to the draw off valve inlet of the pump 1 Using the appropriate cutting tool cut a length of Tefzel tubing from the Startup Kit long enough to reach from the In Line Degasser AF to the draw off valve inlet of the pump 2 Attach a compression screw and ferrule included in the Startup Kit to one end of this tubing as described in the Assembling Fittings discussion at the beginning of this chapter 3 Connect this fitting to the channel outlet on the front panel of the In Line Degasser AF that corresponds to the inlet line of the eluent Figure 3 8 42 Making Fluidic Connections To Pump Figure 3 8 Connecting an Outlet Line to the In Line Degasser AF 4 Mark the line with a line marker A or B included in the Startup Kit to identify the reservoir supplying the eluent 5 Cut a piece of 0 149 inch od TFE tubing from the Startup Kit approximately 2 inches 5 cm long
36. ling the Cover 10 Insert the power cord into the receptacle on the rear panel 5 2 8 Testing the Vacuum Pump Test the vacuum pump after you have determined that the vacuum valve vacuum sensor power supply and the control board assembly are operating properly Vacuum Test Caution To avoid possible electric shock do not open the power supply cover The power supply does not contain user serviceable components After performing the electrical test test for vacuum leaks 1 Power down the In Line Degasser AF and remove the power cord from the receptacle on the rear panel Caution To avoid possible electric shock ensure that the degasser is powered down and the power cord is disconnected before proceeding 2 Remove the cover as described in Section 5 2 1 Removing and Installing the Cover 3 Connect the voltmeter to the Vacuum terminals on the rear panel See Section 2 2 2 Vacuum Terminals for the procedure 4 Connect the vacuum tubing from the pump directly to the control board assembly as shown in Figure 5 5 Service 66 Rear Panel 10 Control Board Front Panel Figure 5 5 Vacuum Connections to Test the Vacuum Pump Insert the power cable into the receptacle on the rear panel Power up the In Line Degasser AF Monitor the vacuum signal on the voltmeter If the voltage signal in
37. n of inert gas typically helium Inline Degassing 1 3 Inline methods of degassing operate within the chromatographic fluid path The Waters In Line Degasser AF operates between the eluent reservoirs and the inlet to the pump Because degassing occurs close to the pump this method minimizes reabsorption of ambient gas into the eluent The flow rate of eluent through an In Line Degasser determines the efficiency of the degassing At low flow rates most of the dissolved gas is removed as the eluent passes through the vacuum chambers At higher flow rates lesser amounts of gas per unit volume of eluent are removed Section 4 2 2 Degassing Efficiency discusses the efficiency of the In Line Degasser with respect to the flow rate Installation Site Requirements 23 Install the In Line Degasser AF at a site that meets the requirements in Table 1 1 Table 1 1 Installation Site Requirements Parameter Requirement Ambient temperature 4 to 40 C 39 to 104 F Relative humidity 10 to 90 noncondensing Bench space 2 inches 5 cm clearance at rear Zero clearance on the sides and top To prevent instability make sure that the four rubber feet of the degasser are secure on the bench top Inline degassing is most effective when the fluid lines from the degasser to the pump are as short as possible Long sections of polymeric tubing allow gases to dissolve back into the degassed eluents Introduction
38. n the power supply cover The power supply does not contain user serviceable components If the control board is faulty use the following procedure to replace the control board 1 Power down the In Line Degasser AF and remove the power cord from the receptacle on the rear panel Caution To avoid possible electric shock ensure that the degasser is powered down and the power cord is disconnected before proceeding 2 Place the new control board next to the old control board 3 Remove each connector from its receptacle on the old control board and reconnect it to the corresponding receptacle on the new control board and install the sensor tubing The sensor tubing is FEP lined Tygon with an FEP insert If you need to replace this tubing use only the correct part number Refer to Appendix B Spare Parts for the correct part number STOP Attention To avoid damage to the sensor use great care when you remove 65 Troubleshooting and Service 4 Remove the sensor tubing from the old control board and install it on the new control board 5 Remove the M3 x 6 TORX screw and MG star washer from the upper left corner of the old control board 6 Press the old control board off the posts at the other three corners 7 Press the new control board onto the posts 8 Reinstall the M3 x 6 TORX screw and M3 star washer in the upper left corner of the control board 9 Reinstall the cover as described in Section 5 2 1 Removing and Instal
39. nnections between the In Line Degasser AF and a voltmeter as indicated in the following table Degasser Terminal Voltmeter Vacuum Vacuum Gnd Not connected 2 Set the voltmeter to the 5 Vdc range Refer to Chapter 5 Troubleshooting and Service for further details on using the Vacuum terminals for troubleshooting Connecting to Other Instruments 34 Chapter 3 Making Fluidic Connections This chapter describes how to make fluidic connections between the Waters In Line Degasser AF and Waters 600 Series Pumps Waters 510 and 515 HPLC Pumps Waters 1515 and 1525 HPLC Pumps Non Waters pumps Required Materials To make fluidic connections to the In Line Degasser AF you need the following materials Tubing cutter as appropriate for the type of tubing razor knife or blade or file with a cutting edge Tubing either 1 8 inch outside diameter od thick walled Tefzel included in the Startup Kit or 1 16 inch od stainless steel Four ferrules and compression screws included in the Startup Kit for each channel Tubing 0 149 inch od TFE included in the Startup Kit Assembling Fittings The fluidic connectors on the front panel of the In Line Degasser AF are 1 4 28 flat bottom fittings The Startup Kit contains a supply of compression screws and ferrules that you use to make connections with these fittings To assemble each connection 1 Use the appropriate to
40. nsert the fuse holder into the receptacle and snap it into place Voltage Selection and Fuse Installation 28 2 2 Connecting to Other Instruments The In Line Degasser AF can be enabled disabled and monitored externally through electrical connections to other instruments You make these connections through the rear panel connector of the degasser Figure 2 2 The connector allows signal output to and signal inputs from other instruments Table 2 2 describes the function of each terminal on the rear panel connector Rear Panel Connector Figure 2 2 Rear Panel Connector 29 Making Electrical Connections 2s Table 2 2 Rear Panel Terminals Connector Function Refer to External Control A switch closure signal applied to these Section 2 2 1 and terminals disables the operation of the Connecting the In Line Degasser AF A switch open signal External Control applied to these terminals enables the Terminals operation of the In Line Degasser AF Chassis ground N A Vacuum Provides a dc voltage output that is Section 2 2 2 and proportional to the vacuum in the degasser Vacuum Terminals Chassis ground N A Connecting the External Control Terminals Attention To meet the regulatory requirements of immunity from external electrical disturbances that may affect the performance of this instrument do not use cables longer than 9 8 feet 3 meters when you make
41. ny of the following Waters 600 Series Pumps 600 616 626 Position the In Line Degasser AF on the right side of the pump as shown in Figure 3 2 For information about selecting a suitable installation site refer to Section 1 3 Installation Site Requirements The In Line Degasser AF is most effective when the fluid lines from the degasser to the gradient proportioning valve GPV or pump inlet are as short as possible Long sections of polymeric tubing allow ambient gases to dissolve back into the degassed eluents Install the In Line Degasser AF between the eluent reservoirs and the GPV TP01172 Figure 3 2 Positioning the In Line Degasser AF in a Waters HPLC System Connecting Inlet Lines To connect an eluent line from the In Line Degasser AF to an eluent reservoir 1 Using the appropriate cutting tool cut a length of Tefzel tubing from the Startup Kit just long enough to reach from the In Line Degasser AF inlet to the eluent reservoir Installing with Waters 600 Series Pumps 37 2 Install a compression screw and ferrule from the Startup Kit to one end of this tubing as described in the Assembling Fittings discussion at the beginning of this chapter 3 Connect the end of the tubing with the compression screw and ferrule to an available inlet fitting on the In Line Degasser AF 4 Insert the other end of the tubing into the cap of the eluent reservoir as s
42. ol to cut the tubing to the required length Make sure the end is straight and free from burrs or debris 1 Polytetrafluoroethylene 35 2 Slide the compression screw over the tubing end followed by the ferrule as shown in Figure 3 1 Be sure the tapered end of the ferrule faces away from the end of the tubing Compression Screw Ferrie Tubing Tubing End Straight and Smooth Figure 3 1 Ferrule and Compression Screw Assembly Making Connections To make a tubing connection to the In Line Degasser AF inlet or outlet 1 Firmly seat the tubing end in the appropriate fitting 2 Complete the connection by tightening the compression screw until it is finger tight Attention To avoid damaging the ferrule do not overtighten the compression screw Pressure Limit The degassing membrane in the Waters In Line Degasser AF can withstand a maximum pressure of 10 psi 70 kPa Attention To avoid damaging the In Line Degasser AF do not apply more than 10 psi 70 kPa to the eluent reservoirs Increasing Efficiency To minimize the equilibration time for the In Line Degasser AF fill the degas tubing in any unused vacuum chambers with a fluid such as water or eluent Install the caps on the inlet and outlet of each fluid filled chamber to prevent leaks 36 Making Fluidic Connections 3 1 Installing with Waters 600 Series Pumps This section describes how to connect the In Line Degasser AF in HPLC systems that include a
43. oltage is above 15 75 V or below 14 25 V the power supply is faulty Replace the power supply as described next in Replacing the Power Supply Reinstall the power connector into the power receptacle on the control board Reinstall the cover as described in Section 5 2 1 Removing and Installing the Cover Insert the power cord into the receptacle on the rear panel Troubleshooting and Service Replacing the Power Supply If the power supply is faulty use the following procedure to replace the power supply 1 N 2 N o on A co Power down the In Line Degasser AF and remove the power cord from the receptacle on the rear panel Caution To avoid possible electric shock ensure that the degasser is powered down and the power cord is disconnected before proceeding Remove the two M3 x 6 TORX screws on the bottom panel and the M3 x 6 TORX screw on the rear panel Lift the power supply out of the chassis The control board remains attached to the supply Remove the three M3 x 6 TORX screws that secure the cover of the housing Remove the cover Remove the two connectors on the power supply board inside the housing Remove the four M3 x 6 TORX screws that secure the power supply board to the housing The screw in the lower left corner has a star washer beneath it Remove the power supply board from the power supply housing 9 Install the new power supply board into the housing 11 13 1
44. onents Power supply Converts the ac voltage to dc voltages that are used by the control board and the pump Control board Contains the circuits that perform the following tasks Monitor the vacuum Turn on the vacuum pump and control the vacuum pump speed Control the two color front panel LED LED Indicates the status of the In Line Degasser AF Section 4 2 In Line Degasser AF Operation and Table 5 1 describe the operating statuses and the LED indicator 19 Introduction 1 2 Theory of Operation 1 2 1 This section presents information on the following subjects Operating principles Effects of dissolved oxygen in the mobile phase Methods of removing gases from eluents Operating Principles The Waters In Line Degasser AF operates on the principle of Henry s Law to remove dissolved gases from the eluent Henry s Law states that the mole fraction of a gas dissolved in a liquid is proportional to the partial pressure of that gas in the vapor phase above the liquid If the partial pressure of a gas on the surface of the liquid is reduced for example by evacuation then a proportional amount of that gas comes out of solution The In Line Degasser AF uses a gas permeable polymer membrane channel to carry the eluent through the vacuum chamber When the eluent enters the vacuum chamber the vacuum maintains a large differential in gas concentration across the membrane This accelerates the rate at which t
45. oratory practices when handling eluents Wear safety using Refer to the Material Safety Data Sheet for each eluent in use To disconnect the eluent lines 1 Place the eluent reservoir below the level of the inlet fitting on the In Line Degasser AF 2 Loosen the inlet compression screw on the In Line Degasser AF The eluent in the tubing drains into the reservoir 3 Remove the inlet tubing from the In Line Degasser AF 4 Return the eluent reservoir to its normal position 5 Remove the outlet fitting on the In Line Degasser AF or the inlet fitting on the pump whichever is higher 6 Drain the tubing into a suitable waste container 7 Remove the outlet fitting on the In Line Degasser AF or the inlet fitting on the pump 5 2 3 Purging the Eluent Tubing in Vacuum Chambers glasses and gloves Know the chemical and physical properties of the eluents you are f Caution Always observe safe laboratory practices when handling eluents Wear safety using Refer to the Material Safety Data Sheet for each eluent in use To purge the eluent tubing in a vacuum chamber 1 Place the eluent reservoir below the level of the inlet fitting on the In Line Degasser AF Service 58 2 Disconnect the eluent inlet line and let the eluent drain into the reservoir 3 Remove the outlet fitting on the In Line Degasser AF or the inlet fitting on the pump whichever is higher 4 Drain the tubing into a suitable waste container 5 Remove the
46. outlet fitting on the In Line Degasser AF if you did not disconnect it in step 3 6 Attach a short piece at least 2 cm of tubing to the outlet fitting on the In Line Degasser AF 7 Insert a syringe into the tubing and draw out any remaining eluent The eluent tubing in the vacuum chamber holds approximately 8 mL of eluent 5 2 4 Draining the Vacuum Tubing and Chambers If the eluent tubing inside a vacuum chamber develops a leak eluent can accumulate in the vacuum chambers and vacuum tubing When this occurs you need to drain eluent from the vacuum system If you find eluent in more than one vacuum chamber do not assume that all the chambers are faulty Test each vacuum chamber separately Refer to Section 5 2 9 Testing the Vacuum Chambers for the procedure for testing a vacuum chamber Before you test each vacuum chamber drain any eluent from the vacuum tubing and vacuum chambers using the procedures that follow Caution Always observe safe laboratory practices when handling eluents Wear safety glasses and gloves Know the chemical and physical properties of the eluents you are using Refer to the Material Safety Data Sheet for each eluent in use Draining the Vacuum Tubing To drain the eluent in the vacuum tubing 1 Power down the In Line Degasser AF and remove the power cord from the receptacle on the rear panel Caution To avoid possible electric shock ensure that the degasser is powered down and the power cord is
47. p connector on the control board 7 Reconnect the vacuum tubing to the new pump Before reconnecting cut off 1 4 to 1 2 inch from the end of each tubing or replace the vacuum tubing This ensures airtight connections 8 Reinstall the cover as described in Section 5 2 1 Removing and Installing the Cover 9 Insert the power cord into the receptacle on the rear panel 5 2 9 Testing the Vacuum Chambers If you suspect that there is a vacuum leak in a vacuum chamber perform the test in this section If there is eluent in the chamber or in the tubing remove the eluent using the procedures in Section 5 2 4 Draining the Vacuum Tubing and Chambers You do not need to remove the eluent lines on the front panel to perform this test Caution To avoid possible electric shock do not open the power supply cover The power supply does not contain user serviceable components 1 Power down the In Line Degasser AF and remove the power cord from the receptacle on the rear panel Caution To avoid possible electric shock ensure that the degasser is powered down and the power cord is disconnected before proceeding 2 Remove the cover as described in Section 5 2 1 Removing and Installing the Cover Service 68 69 3 4 Rear Panel Connect the voltmeter to the Vacuum terminals on the rear panel See Section 2 2 2 Vacuum Terminals for the procedure Set the voltmeter on the 5 Vdc range Identify the vacuum chamber you wan
48. r use great care when you remove 4 Disconnect the sensor tubing at the vacuum manifold as shown in Figure 5 3 5 Insert the power cable into the receptacle on the rear panel Power up the In Line Degasser AF The voltmeter should read 0 5 V until the pump starts Control Board Vacuum Manifold Syringe Rear Front Panel Panel Figure 5 3 Testing the Vacuum Sensor 61 Troubleshooting and Service 6 Insert a syringe into the open tubing Make sure the syringe seals against the tubing 7 Monitor the vacuum signal on the voltmeter while you draw out the plunger of the syringe You should feel resistance as you withdraw the syringe If the voltage increases as you draw out the plunger the sensor is functioning properly Go to step 8 If the voltage remains at 0 5 V as you draw out the plunger the vacuum sensor is faulty Disconnect power and replace the control board assembly as described in Section 5 2 7 Testing the Control Board Assembly The part number for the Control Board is listed in Appendix B Spare Parts 8 Cut a 1 4 to 1 2 inch length from the end of the tubing This ensures an airtight connection 9 Reconnect the tubing 10 Reinstall the cover as described in Section 5 2 1 Removing and Installing the Cover 11 Insert the power cable into the receptacle on the rear panel 5 2 6 Tes
49. r AF Phillips screwdriver 1 4 inch open end wrench or adjustable wrench Syringe 10 mL Tubing cutter razor knife or razor blade TORX TX 10 and TX 20 screwdrivers 1 each Eluent waste container Volt ohmmeter or voltmeter and ohmmeter 5 2 1 Removing and Installing the Cover Caution To avoid possible electric shock do not open the power supply cover The power supply does not contain user serviceable components Removing the Cover To remove the In Line Degasser AF cover 1 Power down the In Line Degasser AF and remove the power cord from the receptacle on the rear panel Caution To avoid possible electric shock ensure that the degasser is powered down and the power cord is disconnected before proceeding 2 Using a Phillips screwdriver loosen the four screws located on the lower four corners of the cover You do not have to remove the screws 3 Slide the cover straight up and off the chassis 57 Troubleshooting and Service Installing the Cover To install the cover 1 Slide the cover over the chassis The screw slots are keyed to ensure that you install the cover correctly 2 Using the Phillips screwdriver tighten the four screws located on the lower four corners of the cover 3 Insert the power cord into the receptacle on the rear panel 5 2 2 Disconnecting Eluent Lines glasses and gloves Know the chemical and physical properties of the eluents you are T Caution Always observe safe lab
50. rical wall outlet Attention To meet the regulatory requirements of immunity from external electrical disturbances that may affect the performance of this instrument do not use cables longer than 9 8 feet 3 meters when you make connections to the screw type barrier terminal strips In addition ensure you always connect the shield of the cable to chassis ground at one instrument only Attention Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment Caution To protect against fire hazard replace fuses with those of the same type and rating protection provided by the equipment may be impaired Caution Use caution when working with any organic polymer tubing under pressure e Always wear eye protection when near pressurized polymer tubing e Extinguish all nearby flames Do not use Tefzel tubing that has been kinked or severely stressed Caution To avoid possible electric shock do not open the power supply cover The power supply does not contain user serviceable components 4 Caution If the equipment is used in a manner not specified in this document the Symbols Used on the In Line Degasser AF Direct current Alternating current Protective conductor terminal Frame or chassis terminal Caution risk of electric shock high voltage Caution or refer to manual p gt
51. rs 600 Series Pumps 39 3 2 Installing with Waters 510 515 or 1515 1525 HPLC Pumps This section describes how to connect the Waters In Line Degasser AF to the Waters 510 515 or 1515 1525 HPLC Pump Position the In Line Degasser AF on the left side of the pump as shown in Figure 3 5 and Figure 3 6 Refer to Section 1 3 Installation Site Requirements The In Line Degasser AF is most effective when the fluid lines from the In Line Degasser AF to the draw off valve inlet are as short as possible Long sections of polymeric tubing allow ambient gases to dissolve back into the degassed eluents Install the in Line Degasser AF between the eluent reservoir and the draw off valve inlet to the pump EWENT UT MMM TP01771 Figure 3 5 Positioning the In Line Degasser AF with the Waters 510 or 515 Pump 515 Shown 40 Making Fluidic Connections TP01770 Figure 3 6 Positioning the In Line Degasser AF with the Waters 1515 or 1525 Pump Connecting Inlet Lines To connect an eluent line from the In Line Degasser AF to an eluent reservoir 1 Disconnect the eluent tubing from the fitting on the pump draw off valve inlet 2 Using the appropriate cutting tool cut off approximately 1 inch 2 5 cm from the end of the tubing 3 Attach a compression screw and ferrule included in the Startup Kit to the end of this tubing as described in the Assemb
52. s 1 1 About the In Line Degasser AF The Waters In Line Degasser AF Figure 1 1 provides HPLC systems with an automatic continuous method of removing dissolved gases from mobile phases Figure 1 1 Waters In Line Degasser AF Two Channel The standard configurations of the Waters In Line Degasser AF provide either two or four independent fluid channels in separate vacuum chambers Each vacuum chamber is fitted with fluid and vacuum connections About the In Line Degasser AF 16 17 Adding More Vacuum Chambers You can add one or two additional vacuum chambers to a two channel In Line Degasser AF for a total of three or four channels Appendix B Spare Parts contains the part number for the vacuum chamber and manifold You can install additional vacuum chambers in your laboratory using common hand tools Benefits of Degassing Removing dissolved gases from the mobile phase improves the performance and reliability of the pump and the detector Dissolved gases in an eluent can result in Outgassing in a piston chamber causing pressure fluctuations flow rate inconsistency and noise in the detector baseline Vapor locked check valve which stops eluent flow from that pump head Outgassing downstream of the column Outgassing can create bubbles that pass into the detector cell causing baseline disruptions such as spikes Optimal operation of the pump and detector provides the following benefits Stable baselines w
53. s between the In Line Degasser AF and a Waters 600 Series Controller as indicated in the following table and illustrated in Figure 2 3 Degasser Terminal 600 Series Controller Terminal Ext Control S1 S2 S3 or S4 Ext Control Gnd Gnd Do not connect 2 Connect the shield of the cable to chassis ground at one end only This minimizes the chance of creating a ground loop which can adversely affect system performance 31 Making Electrical Connections Waters 600 Series Controller Waters In Line Degasser AF CHART 5 a SWITCHES PRESSURE 2 9 m 9 8 al RM a a z Pa o eo STOP FLOW CHART l PRESSURE Figure 2 3 Waters 600 Series Controller Connection 3 Use the Program Events screen on the 600 Series Controller to program the opening and closing of the degasser External Control switch 4 Program the 600 Series Controller to open the External Control switch at least 2 5 minutes before you begin to equilibrate the system 5 Close the External Control switch at the end of the last run to disable the In Line Degasser AF Refer to the Waters 600E Multisolvent Delivery System User s Guide for instructions on using the Program Events screen 2 2 2 Vacuum Terminals The Vacuum terminals provide an analog voltage signal that corresponds to the vacuum in the vacuum chambers You can use the Va
54. s to adjust as it removes gases from the eluents The LED on the front panel indicates the status of the In Line Degasser AF For more information about the operating statuses see Table 5 1 Using the In Line Degasser AF 4 2 2 Degassing Efficiency Effect of Flow Rate The flow rate of eluent through the In Line Degasser AF determines the efficiency with which the degasser removes gases As the flow rate increases the In Line Degasser AF has less time to remove dissolved gases from the eluent Table 4 1 shows the relationship between the flow rate of an eluent water and the concentration of a gas oxygen dissolved in the eluent Table 4 1 Effect of Flow Rate on Final Dissolved Gas Concentration Flow Rate Final Oxygen mL min Concentration ppm 1 lt 1 2 lt 1 3 5 lt 2 3 Methods to Improve Degassing Efficiency If you need to reduce the dissolved gases below the levels shown in Table 4 1 while maintaining the flow rate you can perform any combination of the following techniques Fill the degas tubing in any unused vacuum chambers with fluid Increase the time the eluent is exposed to vacuum by connecting one or more vacuum chambers in series in the In Line Degasser AF Sparge with an inert gas preferably helium in addition to using the In Line Degasser AF Filling Unused Chambers If you do not use all the chambers in the In Line Degasser AF during a run fill the degas tubing in unused vacu
55. scence 21 UV Vis 21 Disabled mode 31 51 Dissolved oxygen 21 Draining vacuum chambers 60 vacuum tubing 59 E Efficiency increasing 36 48 of degasser 23 Electrical connections 26 Waters 600 Series 31 32 Electrical system 19 Electrical troubleshooting 55 Electrochemical detectors 22 Eluent lines connecting 36 disconnecting 58 purging 58 Eluent system 18 Enabled mode 31 50 External control disabled mode 31 51 enabled mode 31 50 terminals 30 using 50 51 Waters 600 Series 31 32 Index 77 F Fittings assembling fluidic 35 Flow rate effect of 48 Fluidic connections connector type 35 fittings 35 line length 37 Non Waters pumps 43 required materials 35 ventline 44 Waters 510 515 1515 1525 Pumps 40 43 Waters 600 Series Pumps 37 39 Fluorescence detectors 21 Fuses IEC and UL CSA rated 27 installing 28 requirements 27 27 H Henry s Law 20 I Inlet lines connecting 510 515 or 1515 1525 Pumps 41 connecting 600 Series Pumps 37 In Line Degasser AF description 16 how to use 46 major systems 18 overview 16 specifications 73 74 standard configuration 16 Inline degassing 23 Inspecting the degasser 24 Installation site requirements 23 Installing cover 58 fuses 28 site requirements 23 vent line 44 with 510 515 or 1515 1525 Pumps 40 with 600 Series Pumps 37 with Non Waters pumps 43 L LED 19 status 47 52 M Major degasser systems electrical 19 eluent 18 vacuum 19 Modes
56. subsidiary or your local Waters Technical Service representative or call Waters corporate headquarters for assistance at 508 478 2000 U S Vacuum Troubleshooting If you suspect that there is a problem with the In Line Degasser AF start with the vacuum troubleshooting tree in Figure 5 1 53 Troubleshooting and Service Vacuum Unstable but Degasser OK Is Enable Yos Degasser Degasser Disabled No Flashing Green Yes Yellow Flashing _____________y Remove Cover Yes Disconnect Eluent Tubing Is Eluent in Vacuum Tubing Purge Eluent from Vacuum Tubing Drain Eluent from Chambers Test Vacuum Sensor Sensor Good Test Pump for Vacuum Leaks Pump Good Yes No Power Up Degasser Go to Electrical Troubleshooting Tree Figure 5 2 Replace Control PCB Assembly Replace Pump Figure 5 1 Vacuum Troubleshooting Tree Troubleshooting 54 Test Vacuum Chambers Chamber Replace Good Chamber Test Vacuum Tubing Replace Tubing Call Waters Service Figure 5 1 Vacuum Troubleshooting Tree Continued Electrical Troubleshooting Use the electrical troubleshooting tree in Figure 5 2 if the LED on the front panel of the In Line D
57. t to test then disconnect the remaining vacuum chambers from the vacuum manifold Plug the open ends of the vacuum manifold with wooden pencils or other tapered objects as shown in Figure 5 6 Plug Control Board Front Panel Vacuum Pump Figure 5 6 Vacuum Connections to Test a Vacuum Chamber Insert the power cable into the receptacle on the rear panel Power up the In Line Degasser AF Monitor the vacuum signal on the voltmeter If the voltage signal increases smoothly and stabilizes the vacuum chamber is in good condition Repeat steps 4 through 7 to test another chamber or proceed to step 8 If the voltage signal is unstable or remains at 0 5 V the vacuum chamber is faulty Replace the vacuum chamber as described in Section 5 2 10 Replacing a Vacuum Chamber Reconnect the tubing to the original configuration Before reconnecting cut off 1 4 to 1 2 inch from the free end of each tubing or replace the vacuum tubing This ensures airtight connections Power down the In Line Degasser AF and remove the power cable from the receptacle on the rear panel Reinstall the cover as described in Section 5 2 1 Removing and Installing the Cover Insert the power cord into the receptacle on the rear panel Troubleshooting and Service 5 2 10 Replacing a Vacuum Chamber To replace a faulty vacu
58. tate Description Unlit Unit powered off Steady yellow color Unit operating with pump at high RPM vacuum level above 47 mmHg 0 91 psiA Usually a brief transitional state during initial pump down Steady green color Unit operating with pump at low RPM vacuum level below 47 mmHg 0 91 psiA Typical operating conditions Green flash 0 5 seconds on and 0 5 seconds off Unit operating but vacuum level unstable Indicates a sudden change in degasser work load Yellow flash 0 5 seconds on and 2 seconds off Unit not operating External control jumper or contact closure installed on rear panel connector J2 Operation will resume when the jumper or contact closure is removed Yellow flash 2 seconds on and 0 5 seconds off Unit not operating Vacuum signal out of usable range indicating an electronic or pressure sensor failure Yellow flash 0 5 seconds on and 0 5 seconds off Unit not operating Vacuum pump down level not reached within 10 minutes of power on or enable Indicates a vacuum leak Alternating yellow and green flash Unit not operating Vacuum pump down level reached but then rose above high limit Indicates a vacuum failure Contacting Waters Technical Service If you cannot resolve a problem using the troubleshooting information in this chapter contact Waters Technical Service at 800 252 4752 U S and Canadian customers only Other customers call your local Waters
59. the eluents in use Caution To avoid exposure to eluent vapors connect the outlet vent on the rear panel of the In Line Degasser AF to a suitable fume hood Check local building and health codes for specific requirements regarding the venting of eluent vapors Section 3 4 Installing the Vent Line describes the procedure to connect a vent line to a fume hood 25 Introduction Chapter 2 Making Electrical Connections This chapter describes how to make all necessary electrical connections to the In Line Degasser AF The Waters In Line Degasser AF operates in any standard laboratory environment The unit requires connections for Electrical power Inlet and outlet fluid lines Fume hood or other suitable vent 2 1 Voltage Selection and Fuse Installation Power Requirements For electrical power the Waters In Line Degasser AF requires One properly grounded ac outlet Correct amperage fuse for your ac voltage The Waters In Line Degasser AF automatically senses the input voltage You do not have to change any settings when you change the input voltage between the ranges indicated in Table 2 1 Voltage Selection and Fuse Installation 26 Fuse Requirements The fuses required for sites where the ac line voltage is 120 V nominal or 230 V nominal are listed in Table 2 1 Table 2 1 Voltage and Fuse Selection Nominal Voltage Voltage Range Vac Vac Fuse Required Type 115 85 to 132 1 6 A time delay
60. ting the Power Supply Before you test the power supply verify that Power is on Degasser is enabled no connection to External Control terminals on rear panel Fuses are good supply If you have any doubts about your ability to safely test the power supply contact f Caution To avoid possible electric shock use extreme caution when testing the power your Waters Technical Service representative To test the power supply 1 Power down the In Line Degasser AF and remove the power cord from the receptacle on the rear panel 2 Remove the cover as described in Section 5 2 1 Removing and Installing the Cover 3 Remove the power connector from the power connector receptacle on the control board as shown in Figure 5 4 Service 62 63 10 Power Connector Receptacle d m 3 2 77 A LIII e NS 9 99 9000 0924 REV A Figure 5 4 Power Connector Receptacle on Control Board Assembly Insert the voltmeter leads into the connector Place the positive lead into the connector terminal with the red wire Place the negative lead into the connector terminal with the black wire Set the voltmeter to the 20 Vdc range Insert the power cable into the receptacle on the rear panel Power up the In Line Degasser AF Check that the voltmeter reads 15 V 0 75 V If the voltage is within this range the power supply is functioning properly Go to step 8 If the v
61. tre ie 75 Table of Contents 11 Preface The Waters In Line Degasser AF Operator s Guide describes the procedures for unpacking installing operating maintaining troubleshooting and servicing the Waters In Line Degasser AF It also includes appendixes describing the specifications and spare parts for the degasser This guide is intended for use by personnel who need to install operate maintain troubleshoot and service the Waters In Line Degasser AF Organization This guide contains the following Chapter 1 Introduction describes the degasser including the theory of operation and provides a safety summary installation site requirements and directions for unpacking the degasser Chapter 2 Making Electrical Connections describes the procedures for making electrical connections Chapter 3 Making Fluidic Connections provides procedures for installing the In Line Degasser AF with Waters pumps and HPLC systems with non Waters pumps Chapter 4 Using the In Line Degasser AF explains how to use the In Line Degasser AF Chapter 5 Troubleshooting and Service describes troubleshooting and service procedures for the In Line Degasser AF Appendix A Specifications lists the specifications of the In Line Degasser AF Appendix B Spare Parts provides a list of recommended and optional spare parts Related Documentation Waters 600E Multisolvent Delivery System User s Guide Provides instructions for using the 600 S
62. tubing as described earlier in Draining the Vacuum Tubing Disconnect the vacuum tubing from the chamber Lift the rear of the vacuum chamber unsnapping it from the mounting bracket Remove the vacuum chamber from the chassis Use a wrench to remove the 1 4 inch plug on the front panel of the vacuum chamber Drain the eluent in the chamber into a suitable waste container Replace the plug Reinstall the chamber into the chassis Cut off 1 4 to 1 2 inch from the end of each tubing or replace the vacuum tubing This ensures airtight connections Service 60 12 Reconnect the vacuum tubing 13 Reinstall the cover as described in Section 5 2 1 Removing and Installing the Cover 5 2 5 Testing the Vacuum Sensor The vacuum sensor is mounted on the control board assembly To test the vacuum sensor 1 Power down the In Line Degasser AF and remove the power cord from the receptacle on the rear panel 2 Remove the cover as described in Section 5 2 1 Removing and Installing the Cover 3 Connect a voltmeter to the Vacuum Level terminals on the rear panel as described in Section 2 2 2 Vacuum Terminals Set the voltmeter to the 5 Vdc range and install the sensor tubing The sensor tubing is FEP lined Tygon with an FEP insert If you need to replace this tubing use only the correct part number Refer to Appendix B Spare Parts for the correct part number STOP Attention To avoid damage to the senso
63. um chamber 1 Remove the eluent lines as described in Section 5 2 2 Disconnecting Eluent Lines Purge the vacuum chamber of eluent as described in Section 5 2 3 Purging the Eluent Tubing in Vacuum Chambers Drain the vacuum tubing and or vacuum chamber if necessary as described in Section 5 2 4 Draining the Vacuum Tubing and Chambers 4 Disconnect the vacuum manifold from the rear of the vacuum chamber 5 Lift the rear of the vacuum chamber unsnapping it from the mounting bracket Remove the vacuum chamber from the chassis Mount the new vacuum chamber on the locating pin on the inside of the front panel 7 Snap the rear of the vacuum chamber into the mounting bracket 8 Reconnect the vacuum manifold to the vacuum chamber 9 Reinstall the cover as described in Section 5 2 1 Removing and Installing the 10 11 Cover Reconnect the eluent lines Insert the power cord into the receptacle on the rear panel Vacuum Tubing Schematics Table 5 2 summarizes the schematics for the vacuum tubing inside the In Line Degasser AF Use these schematics as a guide for replacing the vacuum tubing The tubing lengths shown in the figures are approximate Table 5 2 Tubing Schematics Number of Chambers Refer to 2 Figure 5 7 3 Figure 5 8 4 Figure 5 9 Service 70 Control Board Rear Panel
64. um chambers with a fluid such as water or eluent Install the caps on the inlet and outlet of each fluid filled vacuum chamber to prevent leaks This procedure minimizes the equilibration time for the In Line Degasser AF In Line Degasser AF Operation 48 Connecting Vacuum Chambers To connect two or more vacuum chambers in series 1 Detach the outlet line from the first vacuum chamber 2 Attach this outlet line to the outlet connector on a second available vacuum chamber 3 Using an appropriate cutting tool cut a new piece of Tefzel tubing included in the Startup Kit to a length of approximately 8 inches 20 cm 4 Install a compression screw and ferrule included in the Startup Kit on each end of this tubing 5 Connect one end of the tubing to the outlet of the first vacuum chamber as shown in Figure 4 1 To Pump To Reservoir TP01175 Figure 4 1 Two Vacuum Chambers in Series 6 Connect the other end of the tubing to the inlet of the second vacuum chamber as shown in Figure 4 1 7 Repeat steps 1 through 6 if you want to connect more than two vacuum chambers in series 49 Using the In Line Degasser AF 4 3 Sparging Helium sparging reduces the total dissolved gas in the eluent reservoirs and maintains this condition during operation You can sparge the eluents and use the In Line Degasser AF at the same time Refer to your pump operator s manual for specific instructions on connecting sparge lines

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