TS-100C - User manual
Contents
1. Electrical equipment for measurement control and laboratory use EMC requirements General requirements EN 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use General requirements EN 61010 2 010 Particular requirements for laboratory equipment for the heating of materials EN 61010 2 051 Particular requirements for laboratory equipment for mixing and stirring this product conforms to the requirements of the above Directive s Signature Signature Svetlana Bankovska Aleksandr Shevchik Managing director Engineer of R amp D n A 12 06 L01b A 06 204 x Date Dat Version 2 03 December 2014 How to choose a proper Shaker Rocker Vortex ES 20 60 with heating Applications Microbiology ans Extraction s Cell growing PSU 10i we amp Volume of liquids 10 10 ml Erlenmeyer flasks Cultivation flasks and 50 ml tubes E tate AA SS ee A a Multi Bio RS 24 Applications Multi RS 60 Microbiology Extraction Cell growing J J 1 e x gt a PE V 1 gJ i Applications SSS MR 1 mam Applications T Agglutination gg l Com Extraction i Gel staining m destaining Multi Bio 3D Applications Agglutination Extraction Blot hybridisation Gel staining destaining Sse 88 3 10 ml Petri dishes vacutainers and tubes up to 15 ml DNA an2. CHOW RI es seacasircacasesrvigusesncascen E EENES 1 8 C min e Time of thermoblock heating from 25 C till 37 C cecce 6 min e Temperature calibration option e Calibration coefficient range ceee from 0 936 to 1 063 0 063 6 2 General specifications e Speed setting range ccccceccccccceeeceeeeeececeeeeeseeeeeeeeeneaeeeees 250 1400 rpm e Speed setting resolution ccccececsececeeeecceceeeeeseeaeeeeeeeeeeeeeeeeeeteneees 10 rpm e Maximum speed deviation f r ZOO PIM iiaia n ai Anche tients eerie eed ouster TOR T40 TOI iarsin aa e Digital time setting e Mime Setting resolution 2 0 2 2 cece cececcece cece ce eeeecneaeeeeeeeeeaeceeeeseeaeeeeeeeeeaeeeees 1 min e Maximum continuous operation time ee eeeeeeeeeeeeenteeeeneeeeeees 96 hours recommended interval between operation sessions not less than 8 hours Orit ciriciri iein eea dee esi nee ates ane eee 2mm DIS PAY ce ates s ceecs Sea shee cast tetas each aden a e e 16x2 signs LCD e Current power CONSUMPTION 0 0 2 eee eeee ee eeeee teeter eeteeeeeee 12 V 4 9A 60 W e External power supply unit input AC 100 240 V 50 60 Hz output DC 12 V amp DIMO NSIONS asrar cei deni eine ieee een 205x230x130 mm gt WSIQIU ceee ae a eee a aa EEEN 3 7 kg Accurate within 10 Page 12 Optional Description Weight Catalogue accessories kg number SC 18C Thermoblock for 20x0 5 ml 12x1 5ml tubes 0 7 BS 010143 AK SC 18 02C Thermo
3. D Medical Biological Research amp Technologies TS 100C Thermo Shaker with cooling for microtubes and PCR plates mi 18 02C 20x0 2ml 12x15 St Operating Manual Certificate for version V 3EW Contents Safety Precautions General Information Getting Started Operation Calibration Specifications Maintenance Warranty and Claims OM NOWA RF WN S Declaration of Conformity Page 2 1 Safety precautions The following symbols mean A Caution Make sure you have fully read and understood the present Manual before using the equipment Please pay special attention to sections marked by this symbol A Caution Surfaces can become hot during use GENERAL SAFETY Use only as specified in the operating manual provided The unit should be used if dropped or damaged The unit must be stored and transported in a horizontal position see package label After transportation or storage keep the unit under room temperature for 2 3 hrs before connecting it to the electric circuit Use only standard qualitative tubes Use only cleaning and decontamination methods recommended by the manufacturer Do not make modifications to the design of the unit ELECTRICAL SAFETY Connect only to the external power supply unit with voltage corresponding to that on the serial number label Use only the external power supply unit provided with this product Ensure that the power switch and external power su
4. alysis Medical Biological Research amp Technologies Genome sequence CHB O gt PST 60HL p PST 100HL sel with heating PST 60HL 4 with heating or A Applications ELISA analysis PSU 2T Hybridization MPS 3500 w E lt TS 100 with heating TS 100C with heating and cooling 10 10 ml PCR plates microtest plates and Eppendorf type tubes www biosan lv Biosan Sia Ratsupites 7 build 2 Riga LV 1067 Latvia Phone 371 674 261 37 Fax 371 674 281 01 E mail marketing biosan lv
5. and A keys Fig 3 3 set the necessary temperature increment 0 1 C Pressing the key for more than 3 s will increase the increment Caution Thermoblock rotation finishing does not stop the heating tem perature maintenance process The set parameters can also be changed during operation Program execution 4 6 4 7 4 8 4 9 4 10 4 11 4 12 After the thermal stabilisation of the Thermo Shaker when the set and current temperature readings become the same Insert tubes into the thermoblock sockets or place the microwell plate on the thermoblock and close the lid Press the RPM RUN STOP key Fig 3 4 The thermoblock will start rotating and the timer indicator will start counting up the time interval with 1 min precision Note If the rotation speed is set to zero pressing RPM RUN STOP key will start the timer but the thermoblock will not move After finishing the program after the set time elapses the thermoblock motion will stop and the timer will show the flashing reading STOP accompanied by the repetitive sound signal until the RPM RUN STOP key is pressed If the working time is not set or is reset and the timer indicator in the upper line shows 00 00 pressing the RPM RUN STOP key will start continuous operation of the device with countdown timer in the lower line Actual until the RPM RUN STOP key is pressed again If required there is possibility to restart the timer when it is running Pr
6. block for 20x0 2 ml 12x1 5ml tubes 0 7 BS 010143 CK SC 24C Thermoblock for 24x2 0 ml microtubes 0 6 BS 010143 EK SC 24NC Thermoblock for 24x1 5 ml microtubes 0 7 BS 010143 GK SC 96AC Thermoblock for 96 well microplate for 0 7 BS 010143 AK PCR w o skirt with half skirt low and high profile Replacement parts Description Catalogue number Rubber belt 122x6x0 6 BS 000000 S18 Accurate within 10 A Note TS 100 blocks and TS 100C blocks are not interchangeable i e TS 100C blocks cannot be used on TS 100 and vice versa Biosan is committed to a continuous programme of improvement and reserves the right to alter design and specifications of the equipment without additional notice Page 13 Tihs i 7 3 7 4 8 1 8 2 8 3 8 4 Maintenance If the unit requires maintenance disconnect the unit from the electric circuit and contact Biosan or your local Biosan representative All maintenance and repair operations must be performed only by qualified and specially trained personnel Standard ethanol 75 or other cleaning agents recommended for cleaning of laboratory equipment can be used for cleaning and decontamination of the unit Rubber belt replacement For maintenance of reliable operation of the device the producer recommends to replace rubber belts after 1 5 years or 2000 hours of operation time Disconnect the external power supply unit from the dev
7. ess the TIME RUN STOP key once Fig 3 5 to stop the timer Press the TIME RUN STOP key again to restart the timer The thermoblock motion can be stopped at any time by pressing the RPM RUN STOP key In this case the program realisation and the thermoblock motion will stop and the timer will switch into the STOP mode saving previously set time Press the RPM RUN STOP key to repeat the operation with the same time and speed Caution Thermoblock rotation finishing does not stop the heating tem perature maintenance process After finishing the operation set the power switch located on the rear panel of the unit in position O Off and disconnect the external power supply from electric circuit Page 9 5 1 5 2 5 3 5 4 Calibration The device is pre calibrated at the factory calibrating coefficient is 1 00 for operation with temperatures measured by a sensor installed in the heating block To enter the calibration coefficient hold the TIME RUN STOP key Fig 3 5 pressed for more than 8 sec to activate calibration mode The calibration coefficient will be shown on the display Fig 5 1 Set 1 000 value using the A and W keys Fig 3 3 as shown on Fig 5 1 to restore the factory settings Press the RPM RUN STOP key once to exit the calibration mode Calibration procedure 5 5 5 6 5 7 5 8 5 9 1 Page 10 Install autonomic sensor 0 5 C accuracy into the tubes placed into the block soc
8. hem for future shipment or storage of the unit Examine the unit carefully for any damage incurred during transit The warranty does not cover in transit damage Caution Automatic balancing system in this product produces a light metal like noise when moving the unit which is likely to be heard during unpacking and also during operation acceleration deceleration of the platform Itis anormal occurrence and does not indicate a fault or aloose part Complete set Package contents Standard set TS 100C Thermo shaker Spake mubber belt ritenia eerie aene eaae rerea Ee Ea ER aai EdE EES external power supply unit POWT CO O wiv eed ah aes Operating Manual Certificate 20 0 0 2 cccceeececece cee ceeeeeeeeeeseeeeaeeeeeeenaees 1 copy Optional accessories SC 18C thermoblock for microtubes l e onrequest SC 18 02C thermoblock for microtubes oo eee eeeeeeeeeeeeeees onrequest SC 24C thermoblock for microtubes l e on request SC 24NC thermoblock for microtubes eee eter eeeeeeees onrequest SC 96AC thermoblock for microplate and hex key sec on request 3 3 Setup place the unit upon even horizontal non flammable surface 30 cm away from any flammable materials to provide optimum ventilation ensure clearance around the device 20 cm on rear and side faces remove protective film from the display plug the external power supply unit into the socket at the rear side of the unit Thermoblock installation if the
9. hest expectations of users according to many parameters e Fastreaching of specified mixing speed and maintenance of equal amplitude of rotation throughout the Thermo shaker block e Stability of maintaining the set temperature in a wide range throughout the Thermo shaker s block surface e With the help of the temperature calibration function the user can calibrate the unit approx 6 of the selected temperature to compensate differences in the thermal behaviour of tubes from different manufacturers e LCD display indicates preset and current values of temperature speed and time of operation e Quiet motor operation compact size prolonged service life Functions of heating up to 100 C cooling up to 15 C below room temperature and mixing can be performed both simultaneously and independently i e the unit implements three devices in one 1 Shaker 2 Dry block thermostat 3 Thermo shaker There are five heating and cooling blocks available including a block with a plastic lid for PCR plates All blocks are mutually interchangeable and can be easily installed on thermo shaker The device is applicable in e genetic analyses in extraction of DNA RNAand further sample preparation e biochemistry for studying of enzymatic reactions and processes e extraction of metabolites from cellular material Page 5 3 1 3 2 Page 6 Getting started Unpacking Remove packing materials carefully and retain t
10. ice Remove 4 fixation screws on the device bottom and remove the bottom plate Replace the rubber belt fig 7 Reassemble the device Fig 7 Rubber belt replacement Warranty and Claims The Manufacturer guarantees the compliance of the unit with the requirements of Specifications provided the Customer follows the operation storage and transportation instructions The warranted service life of the unit from the date of its delivery to the Customer is 24 months Contact to your local distributor to check availability of extended warranty If any manufacturing defects are discovered by the Customer an unsatisfactory equipment claim shall be compiled certified and sent to the local distributor address Please visit www biosan lv Technical support section to obtain the claim form The following information will be required in the event that warranty or post warranty service comes necessary Complete the table below and retain for your records Model TS 100 Thermo Shaker for microtubes and PCR plates Serial number Date of sale Page 14 9 Declaration of Conformity Declaration of Conformity Equipment name TS 100C Type of equipment Thermo Shaker with cooling Directive EMC Directive 2004 108 EC Low Voltage Directive 2006 95 EC RoHS 2011 65 EC WEEE 2002 96 EC amp 2012 19 EU Manufacturer SIA BIOSAN Ratsupites 7 build 2 Riga LV 1067 Latvia Applied Standards EN 61326 1
11. kets Set the required temperature in operation mode e g 40 C After the unit reaches the set temperature when the set and current temperature readings equal leave the unit for 30 min for thermal stabilization Let us assume that the readings of independent sensor is 39 C but the display s actual temperature is 40 C Fig 4 Then it is necessary to add 1 C correction set temperature Actual p STOP 0000 40 0 hr min RPM T C Fig 4 Control panel in operation mode Set p STOF 1000 40 Hold TIME RUN STOP key Fig 3 5 pressed for more than 8 sec to activate calibration mode The following parameters will be shown on the display Fig 5 calibration temperature from set mode 2 the internal sensor temp sct 40 0 40 0 1 000 40 0 40 0 calibration E temp with actual temp multiplied by last stored coefficient multiplier coeff actual coefficient Fig 5 Control panel in calibration mode 5 10 AN 5 11 5 12 K Use the temperature with multiplier coefficient readings to set the new temperature value Using the Temp A and W keys Fig 3 3 change the calibration coefficient Fig 6A 1 so that the new temperature value Fig 6A 2 corresponds to the autonomic sensor temperature In our example the calibration coefficient will be 0 974 in range 0 936 up to 1 063 increment 0 001 Caution This calibrating coefficient will correct temperature through al
12. l the operation range After finishing the calibration press the RPM RUN STOP key Fig 3 4 once to save the changes and exit the calibration mode The display will show calibrated temperature as shown on fig 6B 3 and the unit will continue thermal stabilization according to the previously set temperature sct 40 0 40 0 00 00 1000 40 0 0 974 39 0 40 0 STOP 0000 39 0 set temperature calibrating temp with 3 calibrated temperature coaticrent 1 multiplier coeff 2 4 Fig 6 Control panel in calibration and operation mode Page 11 6 Specifications The unit is designed for operation in cold rooms incubators and closed laboratory rooms at ambient temperature from 4 C to 40 C in a non condensing atmosphere and maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C 6 1 Temperature specifications SEWN MANGE wtiiiciade ea A eae iin ie edie 4 to 100 C CONMOM RANGE scepsis n aei iian 15 below RT to 100 C e Setting TESOlUtON niesi eae kak hina NAYEE ae 0 1 C SUADIIY esio enaa ereina o isan eek sce 0 1 C a ACCULACY al 437 Caines Man ai ae e i Aad ie 0 5 C e Uniformity over the thermoblocks at 37 C oo eeeeeeeseeeeeeeeeneeeeeeees 0 1 C e Average heating speed from 25 C to 100 C ee eeeeeeeteeeeees 5 C min e Average cooling speed from 100 C tO RP orsa eaa aerar saveensetavecsavnevceunisbace 5 C min om RIOTS
13. pply are easily accessible during use Do not plug the unit into an ungrounded power socket and do not use an ungrounded extension lead Disconnect the unit from electric circuit before moving Disconnect the external power supply unit from power socket to turn off the unit If liquid penetrates into the unit disconnect it from the external power supply unit and have it checked by a repair and maintenance technician Do not operate the unit in premises where condensation can form Operating conditions of the unit are defined in the Specifications section Page 3 DURING OPERATION Do not leave the operating unit unattended Do not impede the platform motion Do not operate the unit in environments with aggressive or explosive chemical mixtures Please contact manufacturer for possible operation of the unit in specific atmospheres Do not operate the unit if it is faulty or has been installed incorrectly Do not use outside laboratory rooms Do not check the temperature by touch Use a thermometer BIOLOGICAL SAFETY Page 4 It is the user s responsibility to carry out appropriate decontamination if hazardous material is spilt on or penetrates into the equipment 2 General Information TS 100C Thermo shaker provides mixing and temperature control of samples in microtest tubes or PCR plate This model of thermo shaker differs from TS 100 with a possibility of cooling samples down to 4 C Features of TS 100C meet the hig
14. rmoblock is notinstalled Caution Thermoblock installation and replacement have to be performed only when the Power switch is turned off and external power supply is disconnect from the device Choose the thermoblock connect the plug to the contact terminal according to the scheme on Fig 1 1 on the underside of the thermoblock Make sure that the connector is mounted tightly Align the thermoblock so that the warning label A is facing the front of the unit Secure with the four knurled screws fig 2 1 Note When installing SC 96AC thermoblock drive one by one 4 screws for several turns and fix them with an hex key included in SC 96AC thermoblock set Changing blocks Disconnect the external power supply unit from the device Remove the four knurled screws fig 2 1 disconnect the plug fig 1 1 Select the new thermoblock and install it according to the point 3 4 Fig 1 Thermoblock connection Fig 2 Thermoblock set up Page 7 4 Operation Recommendations during operation 4 1 4 2 Please check the tubes microwell plates before using be sure that tubes microwell plates are thermoresistant Don t heat the tubes microwell plates over the melting point of the material they are made of use thermoresisting polypropylene tubes Remember that thin walled tubes have a_ higher thermoconducting factor Tube caps can open under the action of high temperature gt 85 C thus causing sample volume shrinkage or poten
15. tial health risk when working with infected material To prevent such cases it is recommended to use tubes with cap lock of Safe Lock type It is recommended to fill test tubes up to 75 of the rated volume for efficient mixing Connect external power supply unit to a grounded power socket and set the power switch located on the rear panel of the unit to position ON The display will turn on with the upper line Set showing time speed and temperature set earlier and the lower line Actual showing current readings of the same parameters thermoblock temperature C which automatically starts rising according to the temperature set in the upper line The time of temperature stabilisation depends on the initial temperature Setting the parameters 4 3 4 4 Page 8 Use the readings in the upper line of the display Set while setting the required parameters Setting time TIME Using the V and A keys Fig 3 1 set the required working time interval in hours and minutes increment 1 min Pressing the key for more than 3 s will increase the increment Setting speed RPM Using the V and A keys Fig 3 2 set the required speed increment 10 rpm Pressing the key for more than 3 s will increase the increment 4 L 5 00 00 1000 35 0 set ws Bi Z Y STOP 000 35 0 Actual f hr min RPM TECO VY oe TIME RPM T C RUN STOP Thermo Shaker Fig 3 Control panel 4 5 AN Setting temperature T C Using the W
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