Fast LC Column User Manual
Contents
1. NOTICE This document contains references to Varian Please note that Varian Inc is now part of Agilent Technologies For more information go to www agilent com chem te Agilent Technologies Fast LC Column User Manual Guidelines for Optimizing Performance with Pursuit XRs 2 8 amp Pursuit UPS HPLC Columns Small particles especially delivered in small column dimensions shorter lengths internal diameters lt 3 mm are being increasingly used for high speed separations To achieve the high efficiency of separations these columns can deliver the HPLC system has to minimize system dead volume and other band broadening contributions All low volume columns perform best when used with proper attention to the following factors gt Connecting Tubing Use the shortest possible lengths of connecting tubing with narrow internal diameters at most 0 010 inch 0 25 mm I D to connect the column to the injector and the detector cell Varian delivers the Pursuit UPS columns with a pre fitted capillary to connect the column to your auto sampler Please use this adapter to connect the column to your optimized HPLC system Detector Detector flow cells must have a small internal volume preferably less than 2 uL Detector Response Time The detector response time should be set to the fastest setting 0 1 second to minimize peak broadening Injector The injection system should be a low volu2. me design e g Rheodyne Model 8125 Sample Solvent For isocratic separations the sample should be dissolved in the mobile phase or in a solvent that is weaker than the mobile phase For gradient separations the sample should be dissolved in the initial mobile phase or in a solvent substantially weaker than the final mobile phase Samples should be particulate free To remove particulates the sample may be filtered through a 0 2 um filter Please consult the instrument manufacturer for details Injection Volume For isocratic separations the volume of sample injected should be kept as small as possible typically 2 uL or less Sample volumes are less critical for gradient separations especially if the sample is dissolved in a weak solvent Inspiring Excellence ap S VARI gt For ultrahigh pressure Fast LC systems it is recommend that only the highest grade of solvents filtered through a 0 2 um filter are used Please consult the instrument manufacturer for details gt Maximum Pressure Pursuit UPS up to 400 bar 5800 psi gt Maximum Pressure Pursuit XRs 8 up to 1034 bar 15 000 psi Please visit http www varianinc com for ordering information Inspiring Excellence
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