Ettan MicroLC - GE Healthcare Life Sciences
Contents
1. WARNING In case of an emergency situation the mains switch must always be easy to access PPP 1 Connect a mains cable 2 supplied between Ettan microLC and a properly grounded mains socket see Fig 5 1 Do not switch on 2 Connect a mains cable 3 between the autosampler and a mains socket at the rear of Ettan microLC see Fig 5 1 3 Complete the two first sections of the Installation record on page 35 The installation phase of Ettan microLC is now completed Ettan microLC Installation Guide 18 1162 23 Edition AB 21 5 Installation of Ettan microLC 5 3 Connecting the mains cables 22 Ettan microLC Installation Guide 18 1162 23 Edition AB Installation test 6 6 Installation test Mains switch AI av UNICORN 4 01 The installation test checks the function of the solvent delivery and the UV monitoring system of Ettan microLC The installation test can also be used at any time to check the condition of the system e g for calibrating the system regularly or after a prolonged stop Correct gradient formation is tested by producing a linear gradient and a series of concentration steps of Uracil Correct UV monitoring is tested by monitoring the Uracil concentration at 214 nm WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation2. 11 Unpackthe microplate base and put it in place At this stage its position is no critical Waste solvent bottle Microplate base 12 Unpackthe waste solvent bottle and attach it to the Autosampler A 905 and insert the related tubing 13 Unpack the Accessory Box E1 The box contains the following components that should be attached to the system Ettan microLC Installation Guide 18 1162 23 Edition AB 17 5 Installation of Ettan microLC 5 1 Unpacking e Flow cell holder UPC 900 Attach to the side of the valve box see figure below UV flow cell Zn optics Conductivity cell Valve box Flow cell holder UPC 900 e Zn optics with 214 nm filter Attach to the Flow cell holder UPC 900 and connect to the rear of the UPC 900 see figure above e Conductivity cell 0 2 ul Attach to the underside of the Flow cell holder UPC 900 and connect to the rear of the UPC 900 see figure above e UV flow cell 6 75 for the Micro flow configuration default or Fast micro flow configuration and UV flow cell 6 150 for the Standard flow configuration Attach the UV flow cell into the detector housing no specific flow direction and tighten the screw underneath see figure above CAUTION Handle the UV flow cell carefully e Capillaries S5 S6 S8 for the Micro flow configurations Attach the capillaries by using f
3. 3 Select File Printer setup from the Main Menu bar Select the appropriate printer from the list and select Landscape Then click on OK to acknowledge the printer chosen Print Setup 2 m Printer Name PHARMACIA BIDTECHSHNDT TECHS Properties Status Ready Type Tektronix Phaser 340 Where BIOTECHARND1 TECH350C PS Q Comment r Paper Orientation Size Jas v Portrait Source Paper v Landscape Network OK Cancel Newer Cane 4 Return to the Main menu screen E 5 Click on the Instant Run button 6 Select Installation Test in the templates list and make sure that Any is selected for both Technique and For column Ettan microLC Installation Guide 18 1162 23 Edition AB 27 6 Installation test 6 4 Running the installation test method For system Use SYSTEMI v Wizard Template r Template selection Technique Method notes Any TEMPLATE Installation Test version 1 00 06 STRATEGY EULC 100 Template NOTE This template is designed for micro flow configuration only NOTE The compression compensation factor on pump B must be set to 4 6E 10 water Autosampler Injection Autosampler Injection Splitter Autosampler Injection Splitter Trap Autosampler Injection Tray Installation Test to check the function of the solvent delivery the autosampler and the UV monitoring system of ETTANmicroLC
4. Installation TestI00 hhh tn 10096 9596 Gradient result Step response result 14 When the test is finished the printer automatically prints out the chromatogram and the test result Ettan microLC Installation Guide 18 1162 23 Edition AB 29 6 Installation test 6 5 Evaluating the installation test results 6 5 Evaluating the installation test results 6 5 1 Automatic evaluation The system automatically prints out the test result when the test is finished The print out consists of a chromatogram and an evaluation of the test result e If the gradient test result is OK the print out says Gradient linearity accepted e If the step response test result is OK the print out says Step response accepted e Ifthe autosampler peaks test result is OK the print out says Autosampler peaks accepted If any of the evaluated values falls outside the specified range go to section 6 6 Correcting faulty evaluation results 6 5 2 Manual evaluation If you suspect that the automatic evaluation does not give a reliable result a manual evaluation can be done 1 Minimise the run data window to access the Main menu 2 Clickon in the results window and then double click on the Installation Test01 icon to open the result file 3 Maximise the chromatogram window by clicking on El in the upper right corner 4 Clickinthe Curves window with the right mouse button and select Properties
5. 5 Click on the Curves tab and select the UV 214nm check box Deselect the other check boxes 6 Press OK 7 Double click on au in the upper left corner of the chromatogram window and read the absorbance for the steps corresponding to UV_214nm Move the vertical bar to the constant section of each plateau by dragging it Enter the absorbance values in mAU in column 2 in the Step response table of the Test record see page page 29 leaving out the decimals 8 Click on Print under File to print out the chromatogram 30 Ettan microLC Installation Guide 18 1162 23 Edition AB Installation test 6 Evaluating the gradient Place a ruler along the gradient part of curve UV 214nm in the printed report The curve should be linear between 1096 B and 9096 B and void of discontinuities Evaluating the step response Calculate the relative adsorption plateau heights for curve UV 214nm as follows 1 Subtract the base line value 096 B from each of the values in column 2 in the Step response table of the Test record see page page 29 and enter the results in column 3 2 Divide each value in column 3 by the base line corrected value corresponding to 10096 B multiply by 100 and enter the results in column 4 The values of column 4 should all fall within the intervals given in column 5 Evaluating the autosampler peaks 1 Inthe printed report read the Area and Area Peak area values for peaks no 1and 2 2 Fillin the Test record 3 _
6. Check that the values are within the given intervals Ettan microLC Installation Guide 18 1162 23 Edition AB 31 6 Installation test 6 6 Correcting faulty evaluation results 32 6 6 Correcting faulty evaluation results Should any of the evaluated values fall outside the specified range proceed as follows If the system differs from the standard configuration evaluate the result manually If the faulty evaluation result remains continue below 6 6 1 Faulty gradient The mixer is faulty e Disturbances may arise from air in the pump pump valves or bad sealings in the pump Refer to the Pump P 905 User Manual 6 6 2 Faulty step response e If all values are faulty air in the pump or a faulty pump 5 and 95 faulty bad sealing in the pumps 596 faulty pump module B 95 faulty pump module A 6 6 3 Faulty autosampler peak values If values are faulty make sure that the autosampler is properly purged and that all tubing fittings are properly tightened Ettan microLC Installation Guide 18 1162 23 Edition AB 7 Test record Date E ed 71 Gradient test result Gradient linear from 96 B to 7 2 Step response test result Step response table Testrecord 7 B 10 9096 m read md corrected vem value slad interval Conc B value 100 95 94 96 70 69 71 30 29 51 5 4 6 0 7 3 Autosampler peak test
7. Manual Injection Manual Injection Splitter Manual Injection Splitter Trap M jection T anual Injection Trap PLEASE NOTE you need 1 transport vial in position 1 filled with distilled water For column Any 7 Click on Run 8 When the Method window is shown click on Next and then Next again 9 Make sure that the compression compensation value for water 4 6 x 10 Pa is set in Pump P 905 refer to section 6 2 2 Setting compression compensation 10 Click on Start to start the Installation test method Note irrelevant information may be de selected by clicking in the Curves window with the right mouse button and then selecting Properties 11 Click on the Curves tab xi Y Axis Curve Style and Colour Flow Scheme Logbook Run Data Groups Run Data Colour Curves Axis r Display curves Select All Clear All Cancel Help 28 Ettan microLC Installation Guide 18 1162 23 Edition AB Installation test 6 12 Select the following curves to be displayed e UV 214nm e Conc e Pressure useful for trouble shooting the system De select all other highlighted curves 13 The curves may now be monitored on the screen as the test progresses The installation test method run time is approximately 48 minutes Evaluatio alla 00 00 TI allationTest100 0 x J Ele Edt View Integrate Operations Procedures Quantitste Mol Size Window Help TE DOES Installation TestH001hhhhhhhhhk 01 1 UV
8. at 100968 distilled water Check on the pump display that the pressure reading is stable variation lt 5 5 Ifthe pressure is not stable consult the Pump P 905 User Manual for trouble shooting instructions 6 Click on END 6 5 Preparation for the installation test Installation Test Method Guide Buffer A Distilled water Buffer B 7 5 mg l Uracil in distilled water Test flow rate 300 l min Test run time Approximately 48 minutes 1 Prepare a flask containing 100 ml of 7 5 mg l Uracil in distilled water 2 Fill the wells A1 and A2 in a 96 well low microplate 3 Seal the microplate with a foil or a cap mat 4 Immerse inlet tubing B1 into the rest of the Uracil solution 5 Immerse inlet tubing A1 in a distilled water flask gt 200 ml 6 Filla transport solvent vial with distilled water and seal it with a septum and cap 7 Putthis vial in position 1 in the autosampler 26 Ettan microLC Installation Guide 18 1162 23 Edition AB Installation test 6 8 Inthe System Control make sure that plate type 96LOW is selected in Special in System Setting Note It is also possible to fill two vials instead of using a microplate Put the vials in position A1 and A2 and select 48VIALS in System Settings 6 4 Running the installation test method 1 Start UNICORN as described in the Making your first run booklet section 2 2 UNICORN Overview 2 The Toolbar guide is displayed after start up Click on Close
9. consideration the given compressibility for the liquid at the actual pressure Some common values are given below Liquid Value in Pa 1 Water 46 x 10 Methanol 1241 x 10 Most other organic solvents e g ethanol 110x107 Before starting the installation test make sure that the compensation value is set to 4 6 x 10 Pa for water To set the compression compensation 1 Select main menu Setup press OK 2 Select sub menu Setup Compr comp press OK 3 Select On to activate the compensation Press OK 4 Select sub menu Setup Start comp press OK 5 Select On to activate the start compensation Press OK 6 Select sub menu Setup Compr value A press OK 7 Enter compensation value 4 6 x 10 Pa for pump module A 8 Select sub menu Setup Compr value B press OK 9 Enter compensation value 4 6 x 10 Pa for pump module B Ettan microLC Installation Guide 18 1162 23 Edition AB 25 6 Installation test 6 3 Preparation for the installation test 6 2 3 Testing pressure stability Perform a pressure test to establish that all air has disappeared from the pump heads Perform as follows 1 Make sure that inlet tubing A1 and B1 are immersed in a distilled water flask 2 Connectacapillary between port 4 on the flow direction valve and the inlet of the UV optical unit 3 Run 0 2 ml min at 0 B distilled water Check on the pump display that the pressure reading is stable variation lt 5 4 _ Run 0 2 ml min
10. result Autosampler peak table 1 2 3 4 Peak no Area Area Peak area Allowed interval mAU x min 96 90 1 66 1 67 2 2 32 8 33 9 Ettan microLC Installation Guide 18 1162 23 Edition AB 33 7 Test record 7 5 Autosampler peak test result 34 Ettan microLC Installation Guide 18 1162 23 Edition AB 8 Installation record Installation record 8 1 Check Unpacking Contents according to packing lists All packing material removed No visible damage Sign Remarks Installation Injection valve waste tubings port 4 and 5 marked W1 and W2 to waste reservoir Column holder installed Computer and printer installed UniNet 1 cabling installed Mains power cabling installed Installation test Solutions prepared Tubings to piston seal rinsing system in 2096 ethanol Ettan micro LC prepared Installation Test method run Installation Test results evaluated Test Record completed Registration Form completed Test Record and copy of Registration form stored in System Logbook Registration form posted to Service Administration nstallation Guide stored in User Manual box for future use Ettan microLC Installation Guide 18 1162 23 Edition AB 35 8 Installation record 36 Ettan microLC Installation Guide 18 1162 23 Edition AB Registration form 9 x 9 Registration form IMPORTANT WARRANTY REGISTRATION INFORMATION Please ensure that this form i
11. the lamp is ON WARNING Ettan microLC must be connected to a grounded mains socket WARNING There must always be a sample loop connected to ports 2 and 6 of the injection valve This prevents liquid spraying out of the ports when switching the valve which is especially dangerous if hazardous chemicals are being used WARNING Two people are required to lift the system WARNING Never block the flowpath outlet since this will create over pressure and may result in injury WARNING Only spare parts approved or supplied by GE Healthcare may be used for maintaining and servicing the system WARNING Never place waste containers on the top of the system If they become full and overflow liquid may penetrate the system causing a short circuit WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation and maintenance of the system gt gt PPP BRRR Ettan microLC Installation Guide 18 1162 23 Edition AB 9 2 Safety 10 Ettan microLC Installation Guide 18 1162 23 Edition AB Pre requisites 3 3 Pre requisites A WARNING Ettan microLC must be connected to a grounded mains socket e Two people are recommended to lift Ettan microLC onto the working bench e install Ettan microLC a working area of ab
12. vitro use or for diagnostic purposes Ettan microLC is assembled and fully tested before shipping For safe transportation however some components have been secured and thus need to be detached before the system can be tested and used Cables capillaries accessories etc are enclosed in paper boxes This guide describes how to install Ettan microLC The guide is divided into two parts one describing the installation and one describing how to run the installation test After the installation procedure has been performed your Ettan microLC is ready for purification work For full details of specifications methods maintenance etc refer to the respective User Manuals and Instructions Ettan microLC Installation Guide 18 1162 23 Edition AB 1 About this installation guide 8 Ettan microLC Installation Guide 18 1162 23 Edition AB Safety 2 2 Safety e The system is designed for indoor use only e Do not use in a dusty atmosphere or close to spraying water Refer to Technical Specifications in the System Manual for detailed environmental pre requisites WARNING The Ettan microLC system operates under high pressure Eye protection must be worn at all times WARNING The individual modules must not be opened by the user They contain high voltage circuits that can give a lethal electric shock WARNING Monitor UPC 900 uses high intensity ultra violet light Do not disconnect the optical unit while
13. 2 416 8206 Canada Tel 1 800 463 5800 Fax 1 800 567 1008 Central East amp South East Europe Tel 43 1 972 720 Fax 43 1972 722 750 Denmark Tel 45 70 25 24 50 Fax 45 45 16 2424 Eire Tel 1 800 709992 Fax 44 1494 542010 e Finland Baltics Tel 358 9 512 3940 Fax 358 9 512 39439 France Tel 01 69 35 67 00 Fax 01 69 41 98 77 Germany Tel 0800 9080 711 Fax 0800 9080 712 Greater China Tel 852 2100 6300 Fax 852 2100 6338 e Italy Tel 02 26001 320 Fax 02 26001 399 Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 Korea Tel 82 2 6201 3700 Fax 82 2 6201 3803 Latin America Tel 55 11 3933 7300 Fax 55 11 3933 7304 Middle East amp Africa Tel 30 210 96 00 687 Fax 30 210 96 00 693 Netherlands Tel 0800 82 82 82 1 Fax 0800 82 82 82 4 e Norway Tel 47 815 65 777 Fax 47 815 65 666 Portugal Tel 21 417 7035 Fax 21 417 3184 e Russia amp other C I S amp N I S Tel 7 495 956 5177 Fax 7 495 956 5176 Spain Tel 902 11 72 65 Fax 935 94 49 65 e Sweden Tel 018 612 1900 Fax 018 612 1910 Switzerland Tel 0848 8028 10 Fax 0848 8028 11 UK Tel 0800 515 313 Fax 0800 616 927 USA Tel 1 800 526 3593 Fax 1 877 295 8102 imagination at work 18 1162 23 AB 07 2006 K Elanders sterv la 2006
14. GE Healthcare Ettan MicroLC Installation Guide e Ettan Important user information All users must read this entire manual to fully understand the safe use of Ettan MicroLC WARNING The WARNING sign highlights instructions that must be fol lowed to avoid personal injury It is important not to pro ceed until all stated conditions are met and clearly understood CAUTION The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Recycling This symbol indicates that the waste of electrical and elec tronic equipment must not be disposed as unsorted munici pal waste and must be collected separately Please contact an
15. an microLC Installation Guide 18 1162 23 Edition AB Installation of Ettan microLC 5 5 Installation of Ettan microLC Begin by creating a clean and dry working area of 200 x 80 cm that allows easy access Then follow the step by step instructions below and fill in the installation record as you go along see page page 35 Note Some components are packed in Accessory Box E1 Note Some packing lists are included in the paper boxes Note It is important that the filters flow cells and lamps are not handled during unpacking For protection of these items they should remain in their packing materials until required for use 5 1 Unpacking 1 Cutthe straps and remove the plastic foil from the cardboard hood 1 2 After having removed the cardboard hood 1 check the contents against the attached packing list Check also all included boxes Store all the enclosed paper boxes and plastic bags in a convenient nearby place 3 Release and remove the strap 2 holding the system to the pallet 4 Remove the staples holding the bottom support 3 to the pallet 5 Lift Ettan microLC onto the work area Two people are required to lift the system 6 Raisethe system to an upright position 7 TurnEttan microLC on its swivel platform to access the fluid component side of the system Ettan microLC Installation Guide 18 1162 23 Edition AB 15 5 Installation of Ettan microLC 5 1 Unpacking 8 Remove the protective foam
16. and maintenance of the system 6 1 Startup the Ettan microLC system 6 1 1 Start up the Ettan microLC separation unit 1 Switchonthe separation unit using the mains switch located to the left on the base platform 6 1 2 Startup the computer and the UNICORN software 1 Switch on the computer the display and the printer according to the instructions in the manufacturer manuals ND Log into Windows by first pressing Ctrl Alt Del and then clicking on OK 3 When the Windows desktop appears start UNICORN by double clicking on the UNICORN icon 4 Select user default and enter default as password Click on OK x 45 UNICORN logon User name default v Password Ettan microLC Installation Guide 18 1162 23 Edition AB 23 6 Installation test 6 2 Preparation of Pump P 905 5 Click on the System Control button in the task bar System Control button A Start 45 UNICORN Main Menu lt 44 Method E ditor untitled 1 System Control 1 Bjorn 3 Evaluation 6 2 Preparation of Pump P 905 6 2 1 Priming and purging Pump P 905 Q 1 Immerse the rinsing tubing in a flask 1 containing 20 ethanol in distilled water 2 Connect a syringe to the rinsing tubing that is connected to the underside 2 i of the left pump head on pump A Slowly draw rinsing solution to the syringe When rinsing solution starts to enter the syringe continue to draw a few milliliters 3 Loosen the syringe and immerse t
17. and the straps from the system Cut here and remove protective foame Cut here Note Use cutting pliers Be careful not to cut any capillaries by accident Make sure not to lose any of the capillary marking tags 9 Saveallthe original packing material If for any reason the equipment has to be repacked for transportation or otherwise it is important that the system can be safely packed using the original packing material CAUTION Do not lift the Ettan microLC by the valves on the side Instead grasp the unit firmly in the gap between the swivel platform and the base of the main unit and lift 10 Unpack the Autosampler A 905 and place it on top of the system Note Do NOT lift the instrument by the front cover Front cover Locking screw Red cap 16 Ettan microLC Installation Guide 18 1162 23 Edition AB Installation of Ettan microLC 5 The Autosampler A 905 has two transportation safety devices which must be removed e Remove the locking screw holding the front cover It is located at the right side of the front cover see figure above Save the locking screws e Ared plastic cap is attached over the needle washing mechanism below the front cover see figure above Remove the plastic cap and save it CAUTION Make sure that none of the ventilation holes are blocked Blockage of the ventilation holes can cause malfunctioning of the Autosampler A 905 or even damage the electronics
18. authorized representative of the manufacturer for infor N mation concerning the decommissioning of eguipment WARNING This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to make adequate measures Contents Contents 1 About this installation guide 2 Safety 3 Pre requisites 4 Installation overview 5 Installation of Ettan microLC 5411 JUnpackitig 5 notte ete 5 2 Installing the computer and the UniNet cables 5 3 Connecting the mains cables 6 Installation test 6 1 Start up the Ettan microLC system 6 2 Preparation of Pump 905 6 3 Preparation for the installation test 6 4 Running the installation test method 6 5 Evaluating the installation test results 6 6 Correcting faulty evaluation results 7 Testrecord 7 1 Gradient test result s 7 2 Step response test result 7 5 Autosampler peak test result 8 Installation record 9 Registration form Ettan microLC Installation Guide 18 1162 23 Edition AB Contents 6 Ettan microLC Installation Guide 18 1162 23 Edition AB About this installation guide 1 1 Aboutthis installation guide IMPORTANT Ettan microLC is intended for laboratory use only not for clinical or in
19. croLC 5 2 Installing the computer and the UniNet cables 20 5 2 Installing the computer and the UniNet cables 1 Unpackandinstall the computer and printer according to the manufacturer s instructions Place them to the left of the system Do not switch them on CAUTION The mains power to Ettan microLC must be switched OFF before the UniNet 1 cabling is installed 2 Complete the UniNet 1 data communication chain by connecting a UniNet cable between the computer and Pump P 905 CAUTION The UniNet connection to the computer MUST be made to the board with four LEDs 1 3 Connect a UniNet cable between one of the UniNet 1 sockets in the autosampler and a UniNet 1 socket in the Monitor UPC 900 see Fig 5 1 All other UniNet 1 cables are connected at delivery An MS unit used with the Ettan microLC system communicates through serial standard cables with the computer See the documentation of the MS unit to connect the unit Ettan microLC Installation Guide 18 1162 23 Edition AB Installation of Ettan microLC 5 10000000 10000000 11000000 11000000 100000 MS UniNet 1 n s CD Fig 5 1 Connecting UniNet 1 and mains cables 5 3 Connecting the mains cables WARNING Ettan microLC must be connected to a grounded mains socket WARNING Only use mains cables delivered or approved by GE Healthcare
20. he tubing in the rinsing solution 1 Purge Pump P 905 as follows 1 Immerse the inlet tubing of all pump modules with filters in a distilled water flask Note Never place the flask below the level of the pump inlet 2 Connect a male Luer syringe of about 30 ml to the open end of the purge tubing 3 Connect a male Luer connector at the other end of the purge tubing to the left purge valve at pump module A 4 Turnthe purge valve counter clockwise half a turn to open it and slowly draw eluent into the syringe 5 When fluid starts to enter the Purge valve syringe continue to draw a few f milliliters before closing the purge valve Check that there is no visible air left in the inlet tubing Outlet tubing Purge tubing 6 Repeat steps 3 to 5 for the other pump heads 2 T CTTT 24 Ettan microLC Installation Guide 18 1162 23 Edition AB Setup Compr comp off off on Setup Start comp off off on Setup Compr value A 4 6E 10 4 6 Setup Compr value B 4 6E 10 4 6 Installation test 6 6 2 2 Setting compression compensation When running Pump P 950 at high pressures flow losses might occur due to the compression of the eluent Pump P 950 has functions that compensate for these flow losses Note The compensation functions should be activated at all times With the compensation function activated the pump takes into
21. ingertights and sleeves see item 15 below 1 Connect capillary S5 between the flow direction valve port 6 and the autosampler port 6 2 Connect capillary S6 between the autosampler port 1 and the injection valve port 7 3 Connect capillary S8 between the flow direction valve port 4 and the conductivity cell WARNING RISK OF EYE INJURIES The fused silica capillaries are strong and flexible However if sharply bent the capillary may split into small pieces of glass 18 Ettan microLC Installation Guide 18 1162 23 Edition AB Installation of Ettan microLC 5 Note Avoid touching the capillary end Proteins from your finger may harm the measurements Note Whenever attaching a silica capillary to a valve ensure the capillary is levelled with the sleeve as shown in the figure below Do not push in the silica capillary further it may scratch the valve channel plate Fingertight Sleeve Silica capillary Silica capillary levelled with sleeve e Put the capillaries 55 and S6 in the slot on the Autosampler A 905 lid see figure A piece of tape will hold them in place 14 Unpack the Contr CU 900 PCI box It contains the following e PCI board 15 Unpack the Accessory kit Ettan microLC It contains the following e Mains power cable e UniNet cable e Fingertights and sleeves for the capillaries 55 S6 and S8 Ettan microLC Installation Guide 18 1162 23 Edition AB 19 5 Installation of Ettan mi
22. out 200 x 80 cm is required e Ettan microLC requires 100 120 220 240 VAC 50 60 Hz electrical supply with safety grounding e Cutting pliers are recommended for cutting plastic straps e Awaste flask The installation test requires the following solutions e 200 ml of distilled water for priming and purging the pump e 100 ml of 0 4 acetone in distilled water e 100 ml of 20 ethanol in distilled water e 10mg of Uracil Biopur 18 1160 37 10 g pack Ettan microLC Installation Guide 18 1162 23 Edition AB 11 3 Pre requisites 12 Ettan microLC Installation Guide 18 1162 23 Edition AB 4 Installation overview 4 Installation overview Unpack Ettan microLC page 15 Unpack and install the computer and the UniNet cables page 20 Connect mains power cabling page 21 Complete the first two sections of the installation record page 35 Start up the Ettan microLC system page 23 Prepare Ettan microLC for the installation test page 26 Run the installation test method page 27 Evaluate the gradient page 31 Evaluate the step response page 51 Evaluate the autosampler peaks page 31 Complete the test record page 53 Complete the registration form page 57 Complete the final section of the installation record page 55 Store photocopies of all records and forms in the System Logbook Store the Installation Guide in the User Manual box Ettan microLC Installation Guide 18 1162 23 Edition AB 13 4 Installation overview 14 Ett
23. s completed and returned to Service Administration to register the users equipment under warranty Institute comipany usa sa A BT 164 Phone Number ccssssescssssessesseessessscscsseesseeeeers Fax Number oossssssssssrssesessrsrsrsersrnrnrrrrsrrnrnrnrrrrrnrn nr rr nt Date of Installation eene Quote NO eR etd mio E Customer INVOICE NO vise Support Agreement purchased with the instrument Y N Note Fill in serial numbers over leaf Ettan microLC Installation Guide 18 1162 23 Edition AB 37 9 Registration form Components Ettan microLC serial number ttti QTY Part Number Description System rack Serial Number Mixer M 925 Monitor UPC 900 Pump P 905 Autosampler A 905 Valve INV 917 Valve SV 903 A Valve SV 903 B Computer Computer display 38 Ettan microLC Installation Guide 18 1162 23 Edition AB www gehealthcare com GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden Ettan and UNICORN are trademarks of GE Healthcare companies GE imagination at work and GE monogram are trademarks of General Electric Company Windows is ei
24. ther a registered trademark or trademark of Microsoft Corporation in the United States and or other countries All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them GE Healthcare reserves the right subject to any regulatory and contractual approval if required to make changes in specifications and features shown herein or discontinue the product described at any time without notice or obligation Contact your local GE Healthcare representative for the most current information UNICORN Any use of this software is subject to GE Healthcare Standard Soft ware End User License Agreement for Life Sciences Software Products 2006 General Electric Company All rights reserved GE Healthcare Bio Sciences AB a General Electric Company GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Pacific Tel 85 65 62751830 Fax 85 65 62751829 e Australasia Tel 61 2 8820 8299 Fax 61 2 8820 8200 Austria Tel 01 57606 1613 Fax 01 57606 1614 Belgium Tel 0800 73 890 Fax 0
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