INSTRUCTION MANUAL Model F10BCI–6x500y
Contents
1. For certain applications round bottom or conical bottom tubes may also be used with appropriate adapters for support during centrifugation These types of bottles are typically specified with reduced g force rating Check with the manufacturer for the maximum g force rating of your bottle There are several different polymeric materials used in the construction of centrifuge bottles Each material offer different kinds of resistance to solvents pH temperatures environments autoclaving and stress crack Eventually however all of these materials will develop stress cracks from continued use Consult with the bottle manufacturer to select the correct type that will suit your application and deliver desired performance and cost Glass tubes or bottles are rarely used in a high speed rotor However if glass tubes are to be used follow manufacturer s instructions to sure that the material can withstand the required g forces 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 8 04 05B Rev 3 7 8 FIBERLite CENTRIFUGE ROTOR USER MANUAL 6 Rotor Care Cleaning and Disinfectin The rotor body does not need washing and cleaning after every run However periodic washing under warm water using a mild detergent solution will help reduce the amount of salt deposits from spills and permit easy placement of sample bottles in the rotor cavities FIBERLite CENTRIFUGE s specially formulated LabCARETM also known a2. RCF the ratio of the centrifugal acceleration at a specified radius and speed ro to the standard acceleration of gravity g according to the following formula raf RCF 2777 g where ris the radius in millimeters is the angular velocity in radians per second 27RPM 60 and g is the standard acceleration of gravity 9807 mm s Thus the relationship between RCF and RPM is RPM max the position of the liquid in the tube at the maximum distance from the axis of rotation when the rotor is at speed maximum radius in mm min the position of the liquid in the tube at the minimum distance from the axis of rotation when the rotor is at speed minimum radius in mm sedimentation the settling out of particles from a suspension in the earth s field of gravity in the centrifuge this process is accelerated and the particles move away from the axis of rotation sedimentation coefficient s sedimentation velocity per unit of centrifugal force dr 1 S x dt ar supernatant the liguid above the sedimented material following centrifugation Svedberg units S a unit of sedimentation velocity is 15 10 seconds swinging bucket rotor a rotor in which the tubes or bottles are carried in buckets or racks that swing up to the horizontal position during centrifugation sometimes referred to as a horizontal or swing out rotor vertical tube rotor a rotor in which the tubes or bottles are held parallel to the axis of rotation wettabl
3. Rev 3 7 8 1 Safety Marks and Notices CAUTION SAFETY MARK This mark points to an important safety instruction in connection with the operation and handling of the rotor Failure to do so may void warranty or result in damage to the rotor centrifuge and in the loss of samples The operator is instructed to follow the accompanying instructions before use or maintenance of FIBERLite CENTRIFUGE rotors and accessories WARNING SAFETY MARK This mark signifies a critical safety consideration that must be adhered to Failure to do so creates the potential of harm to the rotor centrifuge and even personal injury The operator is strongly urged to strictly follow all instructions that accompany this symbol IMPORTANT SAFETY CONSIDERATIONS Please read before using vour rotor Chemical and Biological Safety Use solutions samples or material that are chemically or biologically active or potentially dangerous mixtures only after you read understand e all safety documents data sheets or other information provided by the solutions samples materials or bio active material parts components instruments and tools involved in the experiment e all relevant federal and local safety regulations and e institute all necessary precautionary and safety steps If a supplier provides any of the biologic products involved in the experiment you must familiarize yourself with all cautionary information available from the sup
4. 29 mm cap 356264 red 29 mm cap 3573597 green 29 mm cap 357360 vellow 29 mm cap 357361 blue 29 mm cap 357362 polypropylene 357007 thickwall open top 29 x 104 mm 50 adapter 356996 10 000 pkg 25 t b 50 mL polypropylene pkg 1 363664 polycarbonate tube 29 x 104 mm 40 adapter 356996 10 000 pkg 25 w snap on cap 50 mL polypropylene pkg 1 i d 29 x 115 mm d adapter 356965 conical tube 50 ml polypropylene pkg 1 10 000 d 17 x 120 mm d adapter 356960 conical tube 15 mL polypropylene pkg 1 10 000 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 04 05B 7 Rev 3 7 8 Tube Max Fill Reguired Accessory Max CANE un fa 9 g round bottom tube uf oe sae ay 10 000 a round bottom tube 14 pe dE adapter Delrin aa 10 000 d round bottom tube le core adapter Delrin AR 10 000 Maximum speeds listed are guidelines only j Polyethylene teraphthalate Polypropylene package of 25 Commercially available Observe manufacturer s speed and fill volume guidelines Delrin is a registered trademark of E l Du Pont de Nemours amp Company To ensure leak proof performance especially at forces greater than 10 000xg or when spinning hazardous materials sealing cap assemblies cap and gasket should be used Other special purpose caps made for low temperature storage or to resist aggressive chemicals are specified as such by their respective manufacturers
5. connecting vacuum tubes that might have been exposed to the sample If not certain call the manufacturer of your centrifuge for instructions Mechanical Limitations and Worker Safety The failure mode of FIBERLite CENTRIFUGE rotors is much different than rotors made from metals In case of a total failure and disruption FIBERLite CENTRIFUGE rotors will cause less damage to the centrifuge than their metallic equivalents However total disruption of a large or high energy rotor may still result in some damage to the centrifuge and loss of samples Make sure you follow instructions in this manual and in other documents provided by manufacturers of the centrifuge bottles and tubes and by suppliers of sample mixtures or reagents Follow all operational procedures correctly for each run Never use a rotor without a lid Super speed or General Purpose rotors not tied down securely on the spindle may jump off and cause damage to the drive shaft and the centrifuge chamber Never attempt to touch or stop a rotor by hand Failure to do so may damage the surface finish of the rotor and over time expose some pointed ends of carbon filaments which may break through the skin like a wooden splinter 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 04 05B 3 Rev 3 7 8 gt Trying to stop a rotor also presents a serious personal hazard loose items like scarves or sleeves of a lab coat can be caught by the spinning rotor and
6. FIBERLite CENTRIFUGE Inc Piramoon Technologies INSTRUCTION MANUAL Model F10BCI 6x500y High Speed Rotor For use only in Beckman Coulter Avanti J or HP centrifuges except the Avanti J HC Not for use in any other Beckman Coulter centrifuge or any other centrifuge Type Fixed Angle Volumetric Capacity 6x 500 mL Maximum Speed 10 000 rom Maximum RCF 17 696 xg Weight 13 2 kg 29 Ib Material of Construction Carbon Fiber Composite Rotor Codes F500 in Avanti J and HP series centrifuges 500 in Avanti J E centrifuges Rotor Code F500 was previously F10 TRotor Code 500 was previously 10 1 422 Aldo Avenue Santa Clara CA 95054 USA 800 PTI FUGE www piramoon com Rev 3 7 8 FIBERLite CENTRIFUGE Rotor Products User Reference Manual Rev 3 7 8 FIBERLite CENTRIFUGE ROTOR USER MANUAL Con ten ts Page A GENERAL INFORMATION 1 Safety Marks and Notices 2 2 Rotor Model Designations 5 3 Characteristics of Composite Materials 6 4 Rotor Preparation 6 5 Tube and Bottle Preparation 6 6 Rotor Care Cleaning and Disinfecting 9 7 In Case of Trouble What To Do 10 8 Product Return Procedure 10 9 Field Rotor Inspection Program FRIP 11 10 Warranty Statement 12 e Glossary including essential centrifugation formulas 14 e Patents and Trademarks Notice 15 B RELATIVE CENTRIFUGAL FORCE RCF Table 16 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 04 05B 1
7. HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT AND EXCEPT AS OTHERWISE PROVIDED IN THIS LIMITED WARRANTY FIBERLite CENTRIFUGE S LIABILITY SHALL NOT UNDER ANY CIRCUMSTANCES EXCEED THE CONTRACT PRICE FOR THE PRODUCTS FOR WHICH LIABILITY IS CLAIMED 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 04 05B 13 Rev 3 7 8 e Glossary Angular velocity rate of rotation measured in radians per second 2x rom 60 or 0 10472 x rpm Anodization an electrochemical coating on aluminum surfaces for corrosion resistance buoyant density the density of a particle in a specific medium centrifugal effect accumulated value of b o dt ty where tis time and o is angular velocity Centrifugal Force in a centrifugal field the force which causes a particle to move away from the center of rotation clearing factor k calculated for FIBERLite CENTRIFUGE rotors as a measure of the rotor s relative pelleting efficiency Te 10 NETTO x o 3600 OR 253303 x IN Fmax lmin k RPM 1000 clearing time t in this simple formula t k s where tis time in hours k is the clearing factor of the rotor and s is the sedimentation coefficient in Svedberg units S density mass per unit of volume g mL density separation a centrifugal separation process based on differences in particle densities differential separation a centrifugal separation process based on differences in size between part
8. NTRIFUGE Piramoon Technologies Inc PTI 6 04 05B Rev 3 7 8 FIBERLite CENTRIFUGE ROTOR USER MANUAL Tube Max Fill Reguired Accessory Max Part i Dimensions Volume Part Speed Number PESO eral and Volume mL ie la Number rom 355605 polycarbonate bottle 69x 160 mm pkg 6 w cap assembly 500 mL 107 nong 40 000 361691 polypropylene bottle 69 x 160 mm pkg 6 w cap assembly 500 mL AAS nong 2 998 polypropylene wide mouth bottle sa r n 250 adapter PET jE 10 000 pg w screw cap prg polycarbonate wide 356013 mouth bottle SA EON Ss adapter PET A 10 000 pkg 6 250 mL pkg 2 W SCrew cap 363647 polycarbonate open 29 x 104 mm 40 adapter 356996 10 000 pkg 25 top tube 50 mL polypropylene pkg 1 i 363664 polycarbonate tube 29 x 104 mm 40 adapter 356996 10 000 pkg 25 w snap on cap 50 mL polypropylene pkg 1 i polycarbonate bottle aE assy wiliquid tight 2 z Pm sa aa A Ji 10 000 cap assy polyallomer bottle var assy w liguid tight 2 ra Snell zad annie 10 000 cap assy 357002 polycarbonate bottle 29x 104 mm 34 adapter 356996 10 000 pkg 25 w screw cap 50 mL polypropylene pkg 1 i 357003 polyallomer bottle 29 x 104 mm 42 adapter 356996 10 000 pkg 25 w screw cap 50 mL polypropylene pkg 1 357005 polypropylene 29 x 104 mm 31 adapter 356996 10 000 pkg 25 thickwall tube 50 mL polypropylene pkg 1 w snap on cap natural
9. TY Effective April 2005 and subject to the conditions specified below the FIBERLite CENTRIFUGE F10BCI 6x500y rotor is warranted against defects of material and workmanship which develop within 7 years after delivery by an authorized FIBERLite CENTRIFUGE distributor provided that investigation and factory inspection by FIBERLite CENTRIFUGE discloses that such defect developed under normal and proper use such use including but not limited to speeds not in excess of the maximum speed of the rotor properly reduced for certain fluid densities fluid gradients tube assemblies and adapters as specified in the instruction manual FIBERLite CENTRIFUGE will correct either by repair or at its discretion by replacement any defects of material or workmanship which develop within the warranty period Furthermore should a centrifuge be damaged due to the failure of a FIBERLite CENTRIFUGE rotor covered by this limited warranty FIBERLite CENTRIFUGE will at its discretion repair or replace the rotor and will assume the cost of repairing the damaged centrifuge CONDITIONS 1 This warranty may not be assigned extended or otherwise transferred to a third party without the prior written consent of FIBERLite CENTRIFUGE 2 This warranty covers rotors only and FIBERLite CENTRIFUGE shall not be liable for damage to accessories or ancillary supplies including but not limited to seals o rings bottles and tubes bottle and tube caps bottle a
10. cause injury gt Excessive vibration of a high speed centrifuge will indicate a grossly unbalanced rotor Stop the run immediately remove all bottles from the rotor and check counterbalancing of the bottles in accordance with the manufacturer of your centrifuge Most high super centrifuges require counterbalancing within 1 0 gram Do not exceed maximum rotor speed under any circumstance Speed reduction may be necessary because of weight considerations of tubes adapters condition of the rotor or the density of the solution being centrifuged Be sure to follow appropriate instructions contained in this rotor manual ag Do not put a rotor covered with moisture on the pre cooled drive spindle or it can freeze into place Never leave the rotor on the hub for long periods Keep mating surfaces of the rotor and spindle clean P If any unusual vibrations sounds or odors occur turn off power to the centrifuge immediately and do not operate the centrifuge until the cause of the improper behavior is determined gt Do not try to lift a rotor by force or by swaying it If not removed immediately rotors may stick to the spindles of some high speed centrifuges due to condensation that can quickly freeze over the spindle Leave the rotor in the centrifuge and wait until the centrifuge comes down to room temperature Then try lifting the rotor again If you still cannot remove the rotor contact Beckman Coulter Field Service for adv
11. e tubes tubes made of a material that is impervious to adherence of solutes e Patents and Trademarks Notice FIBERLite CENTRIFUGE products are covered by a series of U S and International invention patents which protect the proprietary nature of the company s carbon fiber composite rotors as well as to distinguish them from conventional metallic rotors For detailed description of said patents please see the company s web site www piramoon com 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 04 05B 15 Rev 3 7 8
12. hat the rotor has 6 cell cavities with 500 mL capacity each The y designates Enhanced Liquid Containment 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 04 05B 5 Rev 3 7 8 3 Characteristics of Composite Materials A composite structure is a non chemically combined material created by the synthetic assembly of two or more components These components include selected filler or reinforcing agent and a compatible matrix binder i e a resin in order to yield specific characteristics and properties The components of a composite material do not dissolve or otherwise merge completely into each other but they act in concert The components and interface between them can be physically identified and characterized In addition it is the behavior and properties of the interface that generally control the properties of a composite structure The key advantage the composite technology is that it provides wide design flexibility and manufacturing latitude to meet the technical performance and cost demands imposed by different applications The goal in creating a composite structure is to combine similar or dissimilar materials in order to develop specific properties that meet desired requirements From the centrifugation point of view what make composite materials specifically carbon fiber and epoxy combinations highly desirable are that they do not corrode and that they provide significantly better strength to density and
13. ice gt Lifting carrying and reaching in and out of the centrifuge poses the potential of lower back injuries Lift carry and handle the rotor with care with your body in complete balance at all times Ask your institution s safety officer or write to Occupational Safety and Health Administration OSHA for advice Dropping the rotor on the floor from a bench top 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 4 04 05B Rev 3 7 8 FIBERLite CENTRIFUGE ROTOR USER MANUAL distance may render it unusable If a rotor is dropped on the floor you must contact Beckman Coulter Field Service to arrange for a free inspection Never use any abrasive tools to clean the rotor If needed uses soft brushes and wash only with mild soap or detergent solutions High or super speed rotors need to be screwed onto the spindle using the tie down knob on the rotor lid IMPORTANT It is the customer s responsibility to decontaminate disinfect or sterilize the rotor and ensure complete safety before contacting Beckman Coulter Inc for service or inspection 2 Rotor Model Designations FIBERLite CENTRIFUGE high and super speed rotors are named to provide information about them following some conventional designations For example the model name F10BCI 6x500y indicates that the rotor is a Fixed angle high speed rotor capable of achieving a maximum speed of 10 000 rpm equipped with a Beckman Coulter Inc hub and t
14. icles fixed angle rotor a rotor in which the tubes are held at an angle usually 20 to 45 degrees from the axis of rotation isopycnic a method of particle separation or isolation based on particle buoyant density sedimentation equilibrium often done with CsCl gradients particles are centrifuged until they reach a point in the gradient where the density of the particle is the same as the density of the gradient at that point maximum volume the maximum volume to which a tube should be filled for centrifugation sometimes referred to as maximum fill volume or nominal fill volume near vertical tube rotor a rotor in which the tubes are held at a slight angle usually 7 to 10 degrees from the axis of rotation pelleting a centrifugal separation where particles in a sample sediment to the bottom of the tube differential centrifugation differential centrifugation separates particles of different sizes by successive centrifugation steps of progressively higher g force and or longer run duration polyallomer random block copolymer of ethylene and propylene used for some thin wall thick wall ultra centrifuge tubes 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 14 04 05B Rev 3 7 8 FIBERLite CENTRIFUGE ROTOR USER MANUAL rate zonal a method of particle separation based on differential rate of sedimentation using a pre formed gradient with the sample layered as a zone on top of the gradient relative centrifugal field
15. lem is spillage of sample during the run from loosely capped bottles and the resulting imbalance bent shafts and or rotor freezing over the spindle If a bottle develops a significant leak in the middle of the run there could be some damage to the drive assembly and the centrifuge chamber caused by the grossly unbalanced rotor It is strongly recommended therefore that bottles are re used only for the number of times or less if subjected to aggressive chemicals recommended by the manufacturer Do not try to lift a frozen rotor by excessive force or by swaying it If not removed immediately rotors may stick to the spindles of some high speed centrifuges due to condensation that quickly freeze over the spindle In such a case leave the rotor in the centrifuge and let the centrifuge chamber come down to room temperature Then try lifting the rotor again If you still cannot remove the rotor call Beckman Coulter Field Service for assistance 8 Product Return Procedure RETURNING A ROTOR Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc This RGA form may be obtained from your local Beckman Coulter sales office and should contain the following information 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 10 04 05B Rev 3 7 8 FIBERLite CENTRIFUGE ROTOR USER MANUAL e serial number of rotor e history of use ap
16. nd tube adapters or bottle and tube contents 3 This warranty is void if the rotor is operated or maintained in a manner contrary to the instructions in the User s manual for either the rotor or the centrifuge or if the rotor is modified without the written permission of FIBERLite CENTRIFUGE 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 12 04 05B Rev 3 7 8 FIBERLite CENTRIFUGE ROTOR USER MANUAL Rotor Replacement Any rotor claimed to be defective must if requested by FIBERLite CENTRIFUGE be returned to the factory transportation charges prepaid and will be returned to the owner transportation charges collect unless the product is found to be defective in which case FIBERLite CENTRIFUGE will pay all transportation charges DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE LIMITED WARRANTY STATES FIBERLite CENTRIFUGE S ENTIRE AND EXCLUSIVE LIABILITY AND BUYER S EXCLUSIVE REMEDY FOR ANY CLAIM OR DAMAGES OF GOODS OR PARTS THEIR DESIGN SUITABILITY FOR USE INSTALLATION OR OPERATION THIS LIMITED WARRANTY SHALL BE IN LIEU OF ALL OTHER EXPRESS OR IMPLIED WARRANTIES INCLUDING BUT NOT LIMITED TO THE WARRANTY OF FITNESS AND THE WARRANTY OF MERCHANTABILITY WHICH ARE HEREIN EXPRESSLY DISCLAIMED FURTHERMORE FIBERLite CENTRIFUGE SHALL HAVE NO LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND WHATSOEVER INCLUDING BUT NOT LIMITED TO LOST PROFITS ARISING OUT OF THE MANUFACTURE USE SALE
17. ou have rotors that have been in use for many years you may wish to have an evaluation conducted at your premises to ensure their continued viability without potential danger of failure For more information on requesting this service from your local representative please call 1 800 742 2345 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 04 05B 11 Rev 3 7 8 10 Warranty Statement FIBERLite CENTRIFUGE INC Piramoon Technologies Inc designs manufactures and sells centrifugation products incorporating advanced composites technology FIBERLite CENTRIFUGE is the exclusive and sole provider of this limited warranty the warranty for its Products offered at the time of purchase FIBERLite CENTRIFUGE s composite rotors are manufactured to be direct replacements of conventional rotors and to run in certain existing models of centrifuges sold by other manufacturers The F10BCI 6x500y rotor has been designed and tested for safety and compatibility when run in the Beckman Coulter Inc Avanti J and HP series centrifuges except Avanti J HC Rotors manufactured of advanced composite materials have performance characteristics and maintenance requirements that are different from rotors of metallic construction The owners are strongly urged to read the instruction manual to familiarize themselves with these requirements Failure to follow the instructions may void the limited warranty provided below LIMITED WARRAN
18. plier s literature with respect to the origin and properties of these products If such information is inadequate or unavailable contact your institution s safety officer 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 2 04 05B Rev 3 7 8 FIBERLite CENTRIFUGE ROTOR USER MANUAL In addition you must keep the following under consideration at all times before during and after centrifugation e Spills may generate aerosols Be familiar with and adhere to safety precautions for aerosol containment Implement aerosol containment particularly when working with virulent organisms such as Hepatitis B and C HIV all forms mycobacteria etc Infectious sample mixtures must be handled according to appropriate laboratory procedures and methods to prevent potentially lethal consequences e Handle all body fluids whether human or animal as if they are capable of transmitting life threatening infectious disease Available laboratory tests do not eliminate the hazard e Follow local and federal guidelines related to health safety and environment to dispose waste solutions e lf at the end of a centrifuge run there is evidence of leakage of biologically active or radioactive samples you should assume that some fluid escaped the rotor In that case you should immediately start appropriate decontamination procedures for the rotor see section 2 3 3 centrifuge and all related accessories such as vacuum pump s and
19. proximate frequency of use e reason for the return e original purchase order number billing number and shipping number if possible name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory and name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that the parts are free from pathogens and or radioactivity Sterilization and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be enclosed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the outside of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label printed on the RGA form when mailing the rotor and or accessories to Beckman Coulter Inc 1050 Page Mill Road Palo Alto CA 94304 Attention Returned Goods Customers located in the United States should call 1 800 742 2345 for assistance Customers located outside the United States should contact their local Beckman Coulter office 9 Field Rotor Inspection Program FRIP Beckman Coulter Inc offers a free Rotor Inspection Program that is available to our customers at no charge If y
20. s FIBERClene is designed to extend the life of your carbon fiber rotor as well as to provide an effective ecologically safe cleaner for general cleaning purposes Please observe the following e Do not use any sharp objects or tools on the rotor and any of its components Use only soft bristle brush to remove dry salts that might be deposited in the cavities or other locations unreachable by hand e The rotor contains some anodized metallic components such as the hub lid knob and tie down screw Do not allow any salts or corrosive chemicals to accumulate over these components Wash them periodically or as required after each run e Regularly check the condition of o rings Replace worn cracked or damaged o rings This rotor utilizes o rings for proper sealing of the cavities to maintain atmospheric pressure in the rotor during the run All rotor o rings and the surfaces of the o ring slots they are placed in must be kept clean and always lightly greased using a silicon vacuum grease O ring refresher kit 393032 includes two lid O rings two tie down screw O rings and 1 oz of silicone vacuum grease e Air dry all rotor components do not wash any rotor components in a dishwasher Do not soak in detergent solutions for long periods i e overnight After some usage the painted and unpainted surfaces of the rotor may develop hairline checking This checking is not structural and will not adversely affect the life and performance of yo
21. stiffness to density ratios than both aluminum and titanium These properties when applied properly result in better safer higher performance centrifugation products 4 Rotor Preparation It is advisable to keep your FIBERLite CENTRIFUGE carbon fiber rotor in a cold room so that the run can start immediately after bottles are placed in the rotor eliminating the need to wait for temperature equilibration in the centrifuge If kept at room temperature FIBERLite CENTRIFUGE rotors will take approximately 1 3 hours to cool down to under 10 C after it is placed in a refrigerator However because carbon fiber rotors provide effective temperature insulation you only need to chill your sample Once placed in the rotor the rotor will maintain up to 95 of the sample temperature throughout the spin cycle It is always important to visually inspect the rotor and its components before and after each run for any unusual nicks checking or other abnormalities See Rotor Care Cleaning and Disinfecting 5 Tube and Bottle Preparation Commonly used rotor bottles have a flat bottom with rounded edges and may be covered with either a screw closure or a sealing cap Other types of bottles with conical or rounded bottoms may be used with inserts Typically these bottles are supplied with a non contaminating screw closure without a liner The following Beckman Coulter tubes bottles and accessories can be used in this rotor 2005 FIBERLite CE
22. ur rotor Disinfecting sterilization decontamination e Cold disinfection methods such as ethanol 70 or bleach 10 are recommended for this rotor However ethanol disinfecting should be done away from the centrifuge at a location i e a vent hood safe for handling flammable liquids Wash all rotor components thoroughly with water to remove residual ethanol bleach or other solutions e Autoclaving is not recommended for this rotor 2005 FIBERLite CENTRIFUGE Piramoon Technologies Inc PTI 04 05B 9 Rev 3 7 8 Note FIBERLite CENTRIFUGE offers no guarantee implied or expressed effectiveness of sterility or disinfecting with respect to procedures and materials mentioned in this document Sterility disinfecting or decontamination methods should be selected in consultation with the laboratory safety officer of your institution e A rotor contaminated with radioactive material must be decontaminated with a solution that will not damage the anodized surfaces of the metallic components or painted surfaces of the rotor body Solutions such as Radcon Surface Spray or IsoClean Solution for soaking and Radiacwash are suitable Most of these solutions are available from most supply catalogues such as those published by VWR Fisher Cole Parmer etc e For regular cleaning and maintenance purposes FIBERLite CENTRIFUGE s LabCARE FIBERClene will suffice 7 In Case of Trouble What To Do The most common prob
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