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1290 Infinity Flexible Cube - User Manual

1. Notices Agilent Technologies Inc 2010 2011 2012 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 64227 90000 Rev B Edition 10 2012 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate wri
2. N 7 3 mY Y 2 P 1 A Ie us oR ae Figure 53 Column Switching Valve Parts 178 1290 Infinity Flexible Cube User M anual Identifying Cables 12 12 Pos 13 Port Valve Head 210 bar Bio inert 12 Identifying Cables Cable Overview 180 Analog Cables 182 Remote Cables 184 BCD Cables 187 CAN LAN Cables 189 External Contact Cable 190 Agilent Moduleto PC 191 Agilent 1200 M odule to Printer 192 This chapter provides information on cables used with the 1290 series of HPLC modules 1290 Infinity Flexible Cube User M anual 179 12 Identifying Cables Cable Overview 180 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EM C regulations Analog cables p n 35900 60750 35900 60750 01046 60105 Remote cables p n 03394 60600 03396 61010 5061 3378 01046 60201 BCD cables p n 03396 60560 G1351 81600 Description Agilent module to 3394 6 integrators Agilent 35900A A D converter Analog cable BNC to general purpose spade lugs Description Agilent module to 3396A Series integrators 3396 Series II 3395A integrator see details in section Remote Cables on page 184 Agilent module to 3396 Series III 3395B integrators Remote Cable Agilent module to general purpose Description Agilent module to 3396 integrators Agilent module to general purpose 1290 Infinity Flexible Cube User M anual Id
3. 86 For best performance of the Autosampler with Flexible Cube Place solvent cabinet with the solvent bottles always on top or at a higher level of the Flexible Cube It is recommended to purge the flush pump before starting a sequence to ensure air free solvent in Autosampler capillaries When changing solvent bottles prime the Flexible Cube to ensure purged solvent lines Use separate solvent bottle for external needle wash in Autosampler e Use inlet filters for flush solvent bottles It is recommended to sonicate solvent 3 which should be set to the chromatographic starting condition before use to avoid generation of air bubbles 1290 Infinity Flexible Cube User M anual Optimizing Lab Performance with Flexible Cube 6 Lia L A el r Ag How to Achieve Lowe How to Achieve Lowest Carry Over Carryover is measured when residual peaks from a previous active containing injection appear in a subsequent blank solvent injection There will be carry over between active injections which may lead to erroneous results The level of carryover is reported as the area of the peak in the blank solution expressed as a percentage of the area in the previous active injection Reducing the Carry over is an important part during any method development process The flush solvents and flush timing has to be adjusted to the sample solvent sample components injected and the mobile phase in use Sampling unit 6 port
4. Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whi d 69761 1290 Infinity Flexible Cube User M anual Hardware Information 13 Electrical Connections The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization One analog output provides signals for integrators or data handling systems The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Ma
5. 223 14 Appendix BCD 187 CAN 189 external contact 190 LAN 189 remote 184 RS 232 191 cables analog 180 BCD 180 CAN 181 external contact 181 LAN 181 overview 180 remote 180 RS 232 181 CAN cable 189 capillaries 163 install 54 cautions and warnings 135 cleaning 138 Communication settings RS 232C 208 compensation sensor open 110 compensation sensorshort 111 condensation 32 configuration Flexible Cube 76 one stack 43 control settings 74 D defect on arrival 40 delivery checklist 41 Diagnostic software 104 dimensions 33 electrical connections 224 1290 Infinity Flexible Cube User M anual descriptions of 197 EM F early maintenance feedback 213 error message position cluster partner missing 119 pressure cluster partner missing 119 valve tag violation 118 error messages compensation sensor open 110 compensation sensorshort 111 cover violation 114 fan failed 111 flexible cube 115 ignition without cover 112 112 initialization of valve failed 117 leaksensoropen 110 leak sensor short 109 leak 112 lost CAN partner 109 remote timeout 108 shutdown 108 timeout 107 valve switching failed 118 evalutation flush pump delivery test 131 external contact cable 190 F fan failed 111 features 10 firmware description 194 main system 194 resident system 194 update tool 195 updates 195 159 159 upgade downgrade 159 upgrade downgrade 159 flow connections 51 1290 Infinity Flexible C
6. Agilent 1200 M odule to Printer 13 Hardware Information Firmware Description 194 Electrical Connections 197 Rear view ofthe module 198 Interfaces 199 Overview Interfaces 202 Setting the 8 bit Configuration Switch without On board LAN 206 Communication Settings for RS 232C 208 Special Settings 210 Instrument Layout 212 Early Maintenance Feedback 213 This chapter describes the module in more detail on hardware and electronics 1290 Infinity Flexible Cube User M anual 193 13 Hardware Information Firmware Description The firmware of the instrument consists of two independent sections e a non instrument specific section called resident system an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are e the complete communication capabilities CAN LAN and RS 232C memory management ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like run synchronization through APG remote error handling diagnostic functions or module specific functions like internal events such as lamp control
7. Stator face assy Bio inert rotor seal PEEK Bearing ring Figure 50 Column Switching Valve Parts 1290 Infinity Flexible Cube User M anual 175 11 Parts and Materials for Maintenance Valve Head 6 Column Selector 600 bar Item p n Description 5067 4146 Valve head 6 column selector 600 bar 1 5068 0089 Stator screws 2 5068 0077 Stator Head 3 5068 0076 Rotor seal PEEK 4 1535 4045 Bearing ring A l S K p W 4 A Y 3 i M SA D S 2 e Figure 51 Column Switching Valve Parts 176 1290 Infinity Flexible Cube User M anual 1290 Infinity Flexible Cube User M anual e e e e e e Valve Head 6 alumn Selector 1200 bar e e e e e Item p n Description 5067 4142 Valve head 6 column selector 1200 bar 1 5068 0089 Stator screws 2 5068 0077 Stator Head 3 5068 0067 Rotor seal Vespel 4 1534 4045 Bearing ring SX i x a Y 4 3 te CS YZ d p 2 8 A gt 1 1 eo f J eae Figure 52 Column Switching Valve Parts ee Agilent Technologies 177 11 Parts and Materials for Maintenance 12 Pos 13 Port Valve Head 210 bar Bio inert For bio inert modules use bio inert parts only inert Item p n Description 5067 4159 Bio inert selector valve 12 position 13 port 210 bar 1 5068 0059 Stator screws 2 5068 0097 Bio inert stator head 3 0101 1288 Bio inert rotor seal and stator face kit 4 1535 4045 Bearing ring re Ny J 4 PZA N RD J aan l S 3 A A y p E
8. 1290 Infinity Flexible Cube User M anual 67 5 68 Using the Module Setting up the Flexible Cube with Agilent ChemS tation LC modules the flexible cube Stoptime stops the flexible cube only and does not stop any other modules Pump Flow Click the down arrow and select the pump flow in mL min from the list Solvent selection For each channel you can select which of the two solvents to deliver The text boxes allow you to type a brief description of the solvents see AO meng md pd om ee e yoo oe mamat hm batt woher 2 08 srs we et ete etat e 2 F Une ght nthe e wetted F Vee cereet vate pete EX G tsesa paia cy a Prater 2 Pet D ah R Fane de e La to lt f a en ee wy O wereeee votre pect Tae Le ee iiae O me egt roo Att gt J we Pi z o Mat he 7 a sran o TemrtsbS 4 100 ewett wen ne Left valve ave Position e Use current valve position Uses the current position of the valve Use valve position Specifies a valve position other than the current one Set the valve position by clicking the down arrow and selecting a position from the drop down list Position after run The Position after run section allows you to select the action to take at the end of the run You can select from e Donotswitch Leaves the valve at its current position 1290 Infinity Flexible Cube User M anual UsingtheModule 5 Switch to position at beginning of run Switches
9. 2 Witha14mmwrench on the flange closest to the pump head unscrew the valve 1290 Infinity Flexible Cube User M anual 141 10 Maintenance and Repair ye Flush Pump Outlet Valve 3 Remove the valve by lifting it upwards 4 Insert the new valve 5 Tighten the valve with a 14 mm wrench attached to the flange closest to the pump head 6 Reconnect the capillary with a 1 4 inch wrench at the outlet of the flush pump 7 Close the front cover and prime the flush pump before use to warrant trouble free usage 142 1290 Infinity Flexible Cube User M anual Exchange Valve Rotor Seal Maintenance and Repair 5 16 inch Hex key 9 64 inch 15 cm long T handle When When rotor seal is visibly damaged blocked or leaks Tools required p n Description 8710 0510 Wrench open 1 4 8710 2394 Parts required p n Description Part number Rotor seal depending on valve pod in use Preparations Open the front door of the module In order to avoid leaks place the solvent bottles at or below the level of the pump head 10 1 Remove all capillaries from the valve head 2 With a 9 64 inch hex key unscrew the stator screws 1290 Infinity Flexible Cube User M anual 143 10 Maintenance and Repair Exchange Valve Rotor Seal 3 Remove the stator head and the stator ring 4 Remove the rotor seal 5 Remove the Isolation seal 6 Replac
10. 80 Using the Module Titanium Ti Titanium is highly resistant to oxidizing acids for example nitric perchloric and hypochlorous acid over a wide range of concentrations and temperatures This is due to a thin oxide layer on the surface which is stabilized by oxidizing compounds Non oxidizing acids for example hydrochloric sulfuric and phosphoric acid can cause slight corrosion which increases with acid concentration and temperature For example the corrosion rate with 3 HCl about pH 0 1 at room temperature is about 13 im year At room temperature titanium is resistant to concentrations of about 5 sulfuric acid about pH 0 3 Addition of nitric acid to hydrochloric or sulfuric acids significantly reduces corrosion rates Titanium is sensitive to acidic metal chlorides like FeClg or CuCly Titanium is subject to corrosion in anhydrous methanol which can be avoided by adding a small amount of water about 3 Slight corrosion is possible with ammonia gt 10 Tantalum Ta Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and acids with free sulfur trioxide It can be corroded by strong bases e g hydroxide solutions gt 10 diethylamine It is not recommended for the use with fluoric acid and fluorides Stainless Steel SST Stainless steel is inert against many common solvents It is stable in the presence of acids and bases in a pH range of 1 to 12 5 It can be co
11. 90 mm length capillary Pos 1 Connection between ports 1 10 2 3 4 5 6 7 8 9 active column 1 left regenerating column right Pos 2 Connection between ports 1 2 3 4 5 6 7 8 9 10 active column 2 right regenerating column left Connect the capillaries connected directly to a column and fasten them immediately with a spanner Finger tighten all remaining capillaries 4 Fasten all fittings with a spanner Starting from position one through six ten respectively fasten the fittings on the valve head Fasten all fittings on attached modules autosampler detector additional pumps Fit all unused valve ports with a plastic plug Place the capillaries that go to another module or waste into the capillary guides to prevent squeezing them when closing the front cover 8 Stow any excess lengths of the capillaries Perform a final leak check 1290 Infinity Flexible Cube User M anual 1290 Infinity Flexible Cube User M anual oe Replacing M duleFirmware When Tools required OR OR Parts required Preparations The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or ifanew module with newer firmware is added to a system or e if third party control
12. Connection between ports 1 6 4 5 2 3 active column 1 left Pos 2 Connection between ports 1 2 3 4 5 6 active column 2 right Example shows setup with flow directed from bottom to top Flow direction from top to bottom needs switch of connected capillaries at ports 5 and 2 Also column inlet connections needed to be switched with outlet connections Port 4 to 3 and 6 to 1 154 1290 Infinity Flexible Cube User M anual Maintenance and Repair 10 Installing the Capillaries wl oe 2 ak 2 3 a oo 7 4 6 SK 8 9 Figure 39 Installing the capillaries for a sample enrichment set up 1 Analytical column 2 280 mm length column length gt 100 mm from analytical column to detector Not pre swaged on column side 3 340 mm length from autosampler and loading pump 4 To waste 5 150 mm length column length up to 100 mm 280 mm length column length gt 100 mm From column Not pre swaged on column side Enrichment column 90 mm length capillary to column 700 mm length 0 17 mm ID from analytical pump oO o N O 90 mm length capillary to column Pos 1 Connection between ports 1 6 4 5 2 3 active column 1 left enrichment column Pos 2 Connection between ports 1 2 3 4 5 6 active column 2 right analytical colum 1290 Infinity Flexible Cube User M anual 155 10 Maintenance and Repair Installing the Capillaries Figure 40 Installing the capillaries for
13. Finde Cube cleaning 7 Enable needle seat back fish Flos ES niian Stoptene Pome Dusrn wi As Purp tioled gt oF m mn lt gt Ok Cancel Figure 24 Method Parameter Settings Flexible Cube cleaning Fendi Cube chasing In order to activate the Flexible Cube cleaning process the Enable neede seat back fush Enable needle seat back flush has to be checked Flexible Cube settings Freebie Cube settings The setup includes choosing what solvent to use Note Fi Soker 1 421 that the Start Cond A1 cannot be deselected Solvert 2 82 Additionally the flow rate and duration can be selected Foes 3 aimn Duration ae 1290 Infinity Flexible Cube User M anual 75 5 Using the Module Module Configuration CAUTION Over pressure in the capillary Damage to the flush pump gt Do not leave the Autosampler to Flexible Cube capillary installed if the Flexible Cube is not part of the Software configuration or if turned off during operation These settings are available via menu Instrument gt More 1290 Infinity ALS gt Control Configuration 76 1290 Infinity Flexible Cube User M anual Using the M odule Setting up the Flexible Cube with Agilent ChemStation f 1100 1200 HipALS Configuration Instrument 1 Z Communication Device name 1290 Infinity ALS Type ID G42264 Serial number DE93055145 Firmware revision 7 06 30 008 Connection settings Options Syringe 20 Seat Capillary 1 2 Max injection v
14. Flexcube fan failed 116 Pump failed at ejecting or atinitializing 116 Valve failed to switch or didn t initialize 117 Initialization of Valve Failed 117 Valve Switching Failed 118 Valve Tag Violation 118 Pressure Cluster Partner Missing 119 Position Cluster Partner Missing 119 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions ee Agilent Technologies 105 8 Error Information W hat Are Error Messages 106 Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook If an error occurs outside a method run other modules will not be informed about this error If it occurs within a method run all connected modules will get a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example l
15. For details please refer to the Agilent 1200 Infinity Series M ethod Development System System M anual G4230 90002 Column Damage or Bias M easurement Results Switching the valve to a wrong position can damage the column or bias measurement results gt Fit the lobe to the groove to make sure the valve is switched to the correct position CAUTION The valve actuator contains sensitive optical parts which need to be protected from dust and other pollutions Pollution of these parts can impair the accurate selection of valve ports and therefore bias measurement results gt Always install a valve head for operation and storage For protecting the actuator a dummy valve head part of Transportation Lock Kit G1316 67001 can be used instead of a functional valve Do not touch parts inside the actuator The tag reader reads the valve head properties from the valve head RFID tag during initialization of the module Valve properties will not be updated if the valve head is replaced while the module is on Selection of valve port positions can fail if the instrument does not know the properties of the installed valve To have the valve correctly recognized by the Agilent Infinity Valve Drive you must have the valve drive powered off for at least 10 seconds 1290 Infinity Flexible Cube User M anual 149 10 Maintenance and Repair Replacing Valve Heads 1 Switch off the module 2 Remove all capillary connections from
16. Parts required p n Description 5067 4717 Inlet valve Preparations e In order to avoid leaks place the solvent bottles at or below the level of the pump head Open the front door of the module 1 Disconnect the finger tight fitting of the Inlet capillary at 2 With a 14mm wrench on the flange closest to the pump the bottom of the Flush pump head unscrew the valve 1290 Infinity Flexible Cube User M anual 139 10 Maintenance and Repair 3 Remove the valve by moving it downwards IS J 4 Insert the new valve 5 Tighten the valve with a 14 mm wrench attached to the flange closest to the pump head Sef big Wp be 6 Reconnect the finger tight fitting at the inlet of the flush pump WG aN 7 Close the front cover and prime the flush pump before use to warrant trouble free usage 140 1290 Infinity Flexible Cube User M anual 10 Maintenance and Repair Exchange Flush Pump Outlet Valve If the Flush pump delivery test fails or if the Outlet valve show signs of malfunction When Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 1924 Wrench open 14mm Parts required p n Description 5067 4716 Outlet valve Preparations Open the front door of the module In order to avoid leaks place the solvent bottles at or below the level of the pump head 1 With a 4inch wrench disconnect the capillary at the top of the Outlet valve
17. Valve Head 6 Column Selector 600 bar 176 Valve Head 6 Column Selector 1200 bar 177 12 Pos 13 Port Valve Head 210 bar Bio inert 178 This chapter provides information on parts and material required for the module 1290 Infinity Flexible Cube User M anual 161 11 Parts and Materials for Maintenance Parts overview Parts overview Item p n Description 1 5067 4717 Inlet valve 2 5067 4716 Outlet valve 3 G4280 67304 Solvent selection valve to flush pump tubing 4 5067 4680 Tubing Kit 600 mm 130 bar 5 5067 4697 Solvent selection valve bridge tubing Figure 42 Parts overview 162 1290 Infinity Flexible Cube User M anual Capillaries Item p n 1 5067 4680 2 G4280 67304 3 5067 4697 1290 Infinity Flexible Cube User M anual Parts and Materials for Maintenance 11 Description Tubing Kit 600 mm 130 bar Solvent selection valve to flush pump tubing Solvent selection valve bridge tubing 163 11 Parts and Materials for Maintenance Accessory Kits HPLC System Tool Kit The HPLC System Tool Kit G4203 68708 contains some accessories and tools needed for installation and repair of the module p n 0100 1681 0100 1710 01018 23702 5023 0240 8710 0060 8710 0510 2x 8710 0641 8710 0899 8710 1534 8710 1924 8710 2392 8710 2393 8710 2394 8710 2409 8710 2411 8710 2412 8710 2438 8710 2509 8710 2594 164 Description Adapter syringe seal wash tube Mounting Tool for Tubing Connections Ins
18. column Backflushing prepares the pre column for the next injection Alternating Column Regeneration Only 2pos 10port valve G4232A B Advantages High sample throughput Increased productivity High efficiency Alternating column regeneration is a convenient way to increase the sample throughput The Agilent 1200 Infinity Series 2 position 10 port valves can be used to increase the efficiency in laboratories running large amounts of samples Gradient elution is a common technique for separation of complex samples in liquid chromatography which requires column regeneration before the subsequent run is started Using alternating column regeneration valuable time for the analysis is saved Core of the alternating column regeneration is the Agilent 1200 Infinity Series 2 position 10 port valves which allows simultaneous analysis on one column while a second identical column is flushed and equilibrated 1290 Infinity Flexible Cube User Manual Introduction 1 Valve solution Valve technique Position 1 Column 2 Eluent 10 pump Autosampler 1 Waste Regeneration Detector pump Column 1 Position 2 Column 2 Eluent pump Autosampler Regeneration gt Detector pump Column 1 Figure 10 Alternating Column Regeneration 1290 Infinity Flexible Cube User M anual 25 1 26 Introduction Valve solution Valve technique With sequential Analysis 1 Regene Analysis 2 Regene Analysis 3 rege
19. B2 Start cond Al Cancel 1290 Infinity Flexible Cube User M anual 73 5 Using the Module Setting up the Flexible Cube with Agilent ChemS tation Control Settings These settings are available via right click on the Active Area of the ALS GUI E Control m jo X Missing Vessel Illumination On Ignore missing vessel Dff Linked Pump G42204 DE92900139 Prime Flush Pump Linked Flexible Cube G42274 PP00000010 Cancel Help NOTE are blocked for other usage 74 Linked Flexible Cube To configure which Flexible Cube delivers flow to the Autosampler Prime Flush Pump Priming the Needle wash port for a given period of time If the Flexible Cube is linked with the pump the flush pump and the solvent selection valve 1290 Infinity Flexible Cube User M anual Using the M odule 5 sith Agilent ChemStation M ethod Parameter Settings These settings are available via Menu gt Instrument gt Setup Agilent 1290 Infinity Autosampler or via right click on the Active area om of G42764 DEVIO55145 i i BP x 1290 Infinity ALS G4226A mmm irector Mode Advanced Ingcchen vole 100 ol Injection Cleaning legection Vahm Dears a 9 Standard npcton inaction with neede wrath Tome mn Broom Terre 2 ma Margats Bypass Need wah Time 3 me Mawrguses B pans Tere 4 mn Margars Eypass Vave movererts ge Finette Cube mitinge Y Sobvert 1 A Solvers 2182
20. Flexible Cube User M anual Installing the Module 3 Optimizing the Stack Configuration Two Stack Configuration Thermostatted Instant Pilot Detector Solvent cabinet Flexible Cube Pump Autosampler Thermostat Column compartment Figure 17 Supported Two Stack Configuration Front View Thermostatted 1290 Infinity Flexible Cube User M anual 47 3 Installing the Module Optimizing the Stack Configuration LAN to control software CAN to Instant Pilot CAN Bus cable AC Power Figure 18 Supported Two Stack Configuration Rear View Thermostatted 48 1290 Infinity Flexible Cube User M anual Installing the M odule Installing the Module Parts required p n Description 64227 64010 Agilent 1290 Infinity Flexible Cube Software required Preparations Power cord Data System and or Instant Pilot G4208A with the appropriate revisions see Performance Specifications on page 34 Locate bench space Provide power connections Unpack the module Module is partially energized when switched off as long as the power cord is plugged in 3 Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instru
21. Table6 Available Tests in Agilent Lab Advisor Test Comment Flush pump delivery test For Flex Cube flush pump Pressure Test For valves For Agilent Instrument Utilities Agilent ChemStation and Agilent Instant Pilot no tests are available for the Flex Cube For details on the use of the interface refer to the interface documentation 1290 Infinity Flexible Cube User M anual TestFunctions 9 Flush Pump Delivery Test The test determines if the flush pump is capable of delivering the required pressure to flush the needle seat The test uses the pressure sensor of the 1290 Infinity binary pump to verify the maximum pressure the flush pump can deliver The flush pump will run against a dead end and therefore produce an audible noise that is normal It produces an error that will be visible in the logbook and will show on the Instant Pilot screen also this is normal The error is automatically restored as soon as the test finishes When In case of a suspected defective flush pump Software required Agilent Lab Advisor Software B 01 04 or higher Preparations G4220A B Binary Pump must be present 1290 Infinity Flexible Cube User M anual 123 9 Test Functions Flush Pump Delivery Test 1 Start Agilent Lab Advisor test screen 2 Click on G4227A in Module View hve Aoao bexrgenm Flush Pump Delivery Test Description The Flexitle Cube Flash Pump Delivery test uses the pressure sensor in the GAZ20 bnary pump
22. Valve 139 Exchange Flush Pump Outlet Valve 141 Exchange Valve RotorSeal 143 Replacing Parts of the Valve Head 146 Replacing Valve Heads 148 Installing the Capillaries 152 Replacing Module Firmware 159 This chapter describes the maintenance of the module 1290 Infinity Flexible Cube User M anual 133 10 Maintenance and Repair Introduction to Maintenance Introduction to Maintenance Figure 37 on page 134 shows the main user accessible assemblies of the Agilent 1290 Infinity Flexible Cube These parts can be accessed from the front simple repairs and don t require to remove the Flexible Cube from the system stack Leak tubing Valve actuator Solvent selection valve for 3 solvents Flush pump outlet valve Flush pump Flush pump inlet valve Power switch Leak sensor not visible in this figure Leak drain Postion for add on valve Figure 37 Front of the Flexible Cube 134 1290 Infinity Flexible Cube User M anual Maintenance and Repair 10 Warnings and Cautions WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volu
23. add on option with a second valve drive is available as well as a delete option without any valve drive if the Flexible Cube is needed for carry over reduction only Other options will follow later 1290 Infinity Flexible Cube User M anual 11 1 Flexible Cube Technical Capability 12 The Flexible Cube is added to the 1290 Infinity system to reduce carry over in the Autosampler This is achieved by flushing the needle seat and rotor seal with up to 3 different solvents which may have different properties and solvent strengths The flush pump outlet capillary is connected to the port 4 of the autosampler which normally holds the waste line If the autosampler is in bypass mode the flush pump connects to the needle seat and can flush backwards through the needle seat into the waste line attached to the needle seat outlet port Besides chemistry effects of solvents and sample the Agilent 1290 Infinity Flexible Cube will not influence injection precision or accuracy of the Autosampler in use The Flexible Cube is not part of the high pressure flow path The needle wash port and the needle seat back flushing function may generate large amount of waste M ake sure to have the waste tubing installed in the autosampler and a waste container with sufficient capacity available 1290 Infinity Flexible Cube User M anual Introduction 1 Flexible Cube Technical Capability Following the injection sequence using a Flexible Cube is described
24. at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed CAUTION Over pressure in the capillary Damage to the flush pump gt Do not leave the Autosampler to Flexible Cube capillary installed if the Flexible Cube is not part of the Software configuration or if turned off during operation This procedure shows the Flexible Cube outside of a system In an Agilent 1290 Infinity Liquid Chromatograph the Flexible Cube is located between a G4220A Binary pump below and the G4226A Autosampler above see Optimizing the Stack Configuration on page 42 1290 Infinity Flexible Cube User M anual 51 3 Installing the Module Flow Connections to the Module for Lowest Carry Over 1 Open the front cover by pressing the buttons on the side of the Autosampler and Flexible Cube modules 2 Install the capillary from the pump outlet into the port 4 of the autosampler injection valve Outlet 3 Install the bottle head assemblies into the solvent selection valve of the Flexible Cube 4 Install low restriction needle seat from accessory kit into autosampler see autosampler manual Maintenance section
25. can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only WARNING The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed 30 1290 Infinity Flexible Cube User M anual CAUTION Site Requirements and Specifications 2 Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate
26. damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1290 Infinity Flexible Cube User M anual In This Guide In This Guide This manual covers the Agilent 1290 Infinity Flexible Cube G4227A 1 Introduction This chapter gives an introduction to the module instrument overview and internal connectors 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Module This chapter gives information about the preferred stack setup for your system and the installation of your Flexible Cube 4 LAN Configuration This chapter provides information on connecting the module to the Agilent Data System 5 Using the Module This chapter provides information on how to set up the Flexible Cube for an analysis and explains the basic settings 6 Optimizing Lab Performance with Flexible Cube This chapter provides information on how to optimize the Autosampler and the Flexible Cube for a minimum carry over and fastest cycle time
27. for procedure 52 1290 Infinity Flexible Cube User M anual Installing the Module 3 Installing the Valve Heads The valve drives are factory installed in the Thermostatted Column Compartment in the Flexible Cube and in the 1290 Infinity Valve Drive The valve heads are interchangeable and can be easily mounted At the first installation the dummy valve has to be removed see Removing the Valve Dummy on page 53 The valve heads can be installed by mounting the valve heads onto the valve drives and fastening the nut manually do not use any tools Be sure that the guide pin snaps into the groove of the valve drive thread Removing the Valve Dummy 1 To remove the valve dummy loosen the nut manually SS SS NN I 1290 Infinity Flexible Cube User Manual 53 3 Installing the Module Installing the Valve Head and Connecting Capillaries For bio inert modules use bio inert parts only Linert CAUTION The valve actuator contains sensitive optical parts which need to be protected from dust and other pollutions Pollution of these parts can impair the accurate selection of valve ports and therefore bias measurement results gt Always install a valve head for operation and storage For protecting the actuator a dummy valve head can be used instead of a functional valve Do not touch parts inside the actuator CAUTION Column Damage or Bias M easurement Results Switching the valve
28. is done via CAN CAN connectors as interface to other modules LAN connector as interface to the control software RS 232C as interface to a computer REMOTE connector as interface to other Agilent products Analog output connector s for signal output 1290 Infinity Flexible Cube User M anual 201 13 Hardware Information 202 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software Some modules have neither on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flex Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card If an Agilent detector DAD MW D FLD VW D RID is in the system the LAN should be connected to the DAD MWD FLD VW D RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropri
29. of the injection valve and needle seat the flow rate chosen should be as high as possible The flow rate might be restricted if using highly viscous wash solvents due to back pressure or if using a very low viscosity wash solvent due to jet stream effects Recommended wash solution for most applications is a water methanol mixture run at 4 mL min flow rate The choice of dissolving solvents greatly depends on analyte and solvents in use Normally the Dissolving solvent 1 should be of a high organic concentration with appropriate modifiers If this is not sufficient to reduce the carry over a dissolving solvent 2 may be used with either different modifiers and or a really strong cleaning solvent Solvent 3 is always set for the final flush and should be the starting condition of the gradient or lower solution strength as this solvent will be in the needle seat and seat capillary at the point of injection Any flushing solvent used must be miscible with previous solvent If this is not the case it can result in precipitations blockages or reduced performance of the instrument 1290 Infinity Flexible Cube User M anual 91 6 Optimizing Lab Performance with Flexible Cube How to Achieve Lowest Carry Over Injection Valve Rinse Cavitations created due to wear will have an effect on the carry over performance of the injection valve By switching the valve back and forth these cavitations will be flushed and cleaned and long term carry over per
30. of the valve drive Whenever you want to power cycle the valve drive for a re boot it needs to be powered off for at least 10 seconds 118 1290 Infinity Flexible Cube User M anual e Rgessureluster Partner Missing 1290 Infinity Flexible Cube User M anual At connection from the valve drive to a defined pressure cluster partner is ost e Probable cause 1 Communication issues 2 Configuration mismatch Position Cluster Partner Missing Probable cause 1 Communication issues 2 Configuration mismatch Suggested actions Check the CAN cable connections of the modules Check and correct if necessary the valve configuration and presence of defined pressure cluster partner Suggested actions Check the CAN cable connections of the modules Check and correct if necessary the valve configuration and presence of defined position cluster partner 2 3 Agilent Technologies 119 8 Error Information Module Error Messages 120 1290 Infinity Flexible Cube User M anual TestFunctions 9 Module Error Messages 9 Test Functions Introduction 122 Flush Pump Delivery Test 123 Test Failed 131 Pressure Test 132 This chapter describes the tests for the module 1290 Infinity Flexible Cube User M anual 121 9 Test Functions Introduction 122 All tests are described based on the Agilent Lab Advisor Software B 02 01 or higher Other user interfaces may not provide any test or just a few
31. off for at least 10 s 1290 Infinity Flexible Cube User M anual 55 3 Installing the Module Installing the Valve Heads 1 The two different pins blue amp green on the valve shaft must fit into the corresponding grooves of the actuator axis The outside pin red must fit into the outside groove 2 It can be possible that you have to turn the valve head until the outside pin completely locks in While turning the valve you should feel some resistance from the drive m COC Otherwise the pins on the valve shaft are not properly fitted into the corresponding adapters on the valve drive 56 1290 Infinity Flexible Cube User M anual Installing the M odule Installing the Valve Heads 3 When the outer pin is locked into the groove manually 4 Install all required capillary connections to the valve screw the nut onto the valve head Fasten the nut manually Do not use any tools M 5 Power on or power cycle your module so the valve head gets recognized during module initialization NOTE Power Off the Infinity valve drive for at least 10 s 1290 Infinity Flexible Cube User M anual 57 3 Installing the Module Installing the Valve Heads 58 1290 Infinity Flexible Cube User M anual 1290 Infinity Flexible Cube User M anual o e ee 4 7 e LAN Configuration g e LAN Configuration 60 This chapter provides information on connecting
32. software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Diagnostic Software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module ot Agilent Technologies 159 10 Maintenance and Repair Replacing M odule Firmware 160 1290 Infinity Flexible Cube User M anual Parts and Materials for Maintenance 11 11 Parts and Materials for Maintenance Parts overview 162 Capillaries 163 Accessory Kits 164 Valve Options Overview 166 2 Pos 6 Port Valve Head 600 bar 168 2 Pos 6 Port Ultra High Pressure Valve Head 1200 bar 169 2 Pos 6 Port Valve Head 600 bar Bio inert 170 2 Pos 10 Port Micro Valve Head 600 bar 171 2 Pos 10 Port Ultra High Pressure Valve Head 1200 bar 172 8 Pos 9 Port Valve Head 600 bar 173 8 Pos 9 Port Ultra High Pressure Valve Head 1200 bar 174 Valve Head 4 Column Selector 600 bar Bio inert 175
33. specified Useselected The pressure limit for the cluster is set to that of the selected cluster partner Autodetect The pressure limit for the cluster is set to that of the module with the lowest pressure limit Right Valve Pressure Limit Cluster see above Illumination Toggles the illumination of the flexible cube On or Off 66 1290 Infinity Flexible Cube User M anual UsingtheModule 5 M ethod Parameter Settings A RA a tice se d Lil too sas ok erates Assembly Usage Use pump and solvent selection valve in method Mark this check box to activate the parameters in the section of the method setup When the check box is cleared the parameters are inactive Use left valve in method Mark this check box to activate the parameters in the section of the method setup When the check box is cleared the parameters are inactive Use right valve in method Mark this check box to activate the parameters in the section of the method setup When the check box is cleared the parameters are inactive Posttime Your instrument remains in a not ready state during the posttime to delay the start of the next analysis You can use the Posttime to allow your column to equilibrate after changes in solvent composition for example after gradient elution Stoptime Stoptime enables you to set a time at which the flexible cube stops an analysis If the flexible cube is used with other Agilent Modular
34. time is selected Volume Enter the required volume of solvent to be delivered in mL in this field The Volume field is available only when Pumpa specified volume is selected Left and Right Valve Position Cluster io Select partners tee panon cluster Type Module type Serial camber lnlermatan gt Flexible Cube right valve GA227A DEBAINO Chasteratle Select all Unselect af Ok Carcel Helo This dialog box allows you to configure multiple valves to switch synchronously It is available only if more than one valve has been configured The table shows all valves that are available as position cluster partners Mark the check boxes against those that you want to add to the position cluster Select all Selects all valves in the list as cluster partners Unselect all Clears all selections If a position cluster has been defined but the method settings use one of the cluster partners the module will remain in a Not Ready state Best working practice the master should always be the valve with the smallest number of ports This avoids conflicts if different valve pods are used 1290 Infinity Flexible Cube User M anual 71 5 Using the Module Setting up the Flexible Cube with Agilent ChemS tation The Flexible Cube User Interface in Autosampler GUI 1290 Infinity ALS a 1290 Infinity BinPump ei Ide ZZ D o O Idie M 0 000 ml min 0 000 ml min Switch off Tray Illumination Edit Wellplate Types R Hexib
35. to Mainpass a p Needle Analytical head Injection valve Solvent selection valve Figure6 Switch back to Mainpass When the complete flushing procedure has finished the autosampler switches back to Mainpass operation and thereby introduce the sample to the flow path 18 1290 Infinity Flexible Cube User M anual Introduction 1 Valve solution Valve technique Agilent 1200 Infinity Quick Change valves support a variety of challenging valve applications The G4227A Flexible Cube hosts up to two valves standard configuration one valve Besides Quick Change valves can be mounted in the external 1290 Infinity valve drive G1170A or in the 1290 Infinity TCC G1316C Examples of typical applications are Dual and multiple column selection Sample enrichment and sample cleanup Alternating Column Regeneration Solvent selection Dual and Multi Column Selection Dual column selection 2pos 6port valve G4231A B and 2pos 10port valve G4232A B Advantages Increase productivity Higher instrument up time Faster method scouting Quickly change between two different stationary phases to check your separation selectivity or use two identical stationary phases to have the second column immediately available after the first one loses efficiency for example with complex matrices 1290 Infinity Flexible Cube User M anual 19 1 Introduction Valve solution Va technique M
36. to test the Selwery of the Fimobie Cute tush pump WOTE T hdaviaegh barf madame orp the system must nude G1720 Binary Pump and a G4226 Hi Petonanes Advert ae ooh un tn OAIT Fleutle Cube Aa the end iof the test the results are evaltumted atometicaty tect paeses d the Sush pump of the C422 Flexible Cobe delvers s prowsure gt 40 ber Figure 31 Lab Advisor Test screen 3 Select Flush Pump Delivery Test 124 1290 Infinity Flexible Cube User M anual TestFunctions 9 Flush Pump Delivery Test 4 Click Run Test Now B 2p Ter hive Pents Figure 32 Flush pump delivery test Test started 1290 Infinity Flexible Cube User M anual 125 9 Test Functions Flush Pump Delivery Test 5 The Flush pump delivery test uses the pressure sensor in the G4220A Binary pump to measure the maximum pressure the flush pump is able to achieve For this the Flexible Cube to Autosampler capillary has to be reconnected from injection valve port 4 to port 6 Flush Pump Delivery Test X Please check whether the capillary has been connected correctly Also choose which port of the solvent selection valve is to be taken and select the purge time that is to be used to purge the flow path Flush pump capillary has been reconnected from port 4 to port 6 in Autosampler injection valve Run test from solvent selection valve position Al v Select the time for purging 1 Min Figure 33 Flush pump delivery test Initial setup dialo
37. uL injected and measured on Agilent 6460 QQQ in specified conditions MRM 1 505 5 170 CE 36 V MRM 3 505 5 201 2 CE 20 V fragmentor 150 V delta EM V 200 V 36 1290 Infinity Flexible Cube User M anual Site Requirements and Specifications 2 Table4 Specifications Agilent Quick Change Valve Heads Kit PN Valve PN Description max Fittings Port Port Liquid pH Range Pressure Volume Contact bar G4230A B 5067 4107 8 pos 9 port high 600 10 32 0 55 uL PEEK SST 0 141 pressure valve head G4230B 5067 4121 8 pos 9 port UHP valve 1200 10 32 0 55 uL Vespel 0 102 head SST G4231A 5067 4137 2 pos 6 port valve head 600 10 32 0 51 uL PEEK SST 0 14 G4231B 5067 4117 2 pos 6 port UHP valve 1200 10 32 0 51 uL Vespel 0 102 head SST G4232A 5067 4144 2 pos 10 port micro 600 M4 89 0 nL PEEK SST 0 14 valve head G4232B 5067 4118 2 pos 10 port UHP 1200 10 32 0 22 uL Vespel 0 102 valve head SST G4234A 5067 4146 6 pos 14 port valve 600 M4 special PEEK SST 0 14 head for column selection G4234B 5067 4142 6 pos 14 port UHP 1200 M4 special Vespel 0 102 valve head for column SST selection G4235A 5067 4159 12 pos 13 port 210 10 32 16 4 uL PEEK 0 141 selection valve head Ceramic bio inert G5631A 5067 4148 2pos 6portvalvehead 600 10 32 1 71 uL PEEK 0 141 bio inert Ceramic G5639A 5067 4134 4 pos 10 port valve 600 10 32 special PEEK 0 14 head for column Ceramic selection bio inert incom
38. with regards to cleaning the sampler 1 Mainpass E MAN Analytical head Needle Needle seat Flush pump Injection valve Solvent selection valve Al A2 B2 Figurel Mainpass The analytical pump is connected to the autosampler sampling loop and the mobile phase is flowing through the autosampler injection loop continuously and thereby cleaning the insides of the tubings 1290 Infinity Flexible Cube User Manual 13 1 Introduction Flexible Cube Technical Capability 2 Switch to Bypass Analytical head g e a l R ten yo Flush pump Injection valve Solvent selection valve A A A a fa Al A2 B2 Figure2 Switch to Bypass The injection valve is switched to bypass removing the injection loop from the high pressure flow path and connecting the flush pump with the needle seat 14 1290 Infinity Flexible Cube User M anual Introduction 1 Flexible Cube Technical Capability 3 Flush with dissolving solvent 1 A2 Needle Analytical head Needle seat E gt gt ee LS Ba J Flush pump r i Injection valve Solvent selection valve AA Al A2 B2 Figure 3 Flush with dissolving solvent 1 A2 The first cleaning solvent available for flushing is normally of a high organic concentration with appropriate modifiers This solvent should be able to dissolve most components of the sample and thereby remove them from t
39. 0 Sound Emission 221 Agilent Technologies on Internet 222 This chapter provides addition information on safety legal and web 1290 Infinity Flexible Cube User M anual 215 14 Appendix General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Symbols Table 20 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up Pie P 216 1290 Infinity
40. 1 Analytical B 2 T4 gt column a Waste b Position 2 Detector Enrichment Pump B column Figure8 Sample enrichment 22 1290 Infinity Flexible Cube User M anual Introduction Valve solution Valve technique PumpA Autosampler Pre column S Waste Analytical column Detector Position 1 Pump B Pump A Autosampler ae Analytical column Detector Position 2 Figure9 Sample clean up 1290 Infinity Flexible Cube User Manual 1 23 1 24 Introduction Enrichment methods Enrichment methods are the technique of choice to obtain highest sensitivity and to remove the sample matrix in such applications as proteomics drug metabolism and environmental trace analysis The analytes are retained and concentrated onto the pre column while the sample matrix is passed to waste After the valve switch a second pump backflushes the analytes out of the pre column onto the separation column This allows injection of large volumes onto the pre column significantly expanding sensitivity in the range of ten to several thousands Stripping methods Stripping methods handle analytes and matrices in the opposite way to enrichment methods Matrix components are retained on the pre column while the analytes pass through to the separation column After the valve switches an additional pump backflushes the matrix components out of the pre column to waste while the analytes are separated on the main
41. 1200 bar Item p n Description 5067 4117 2 pos 6 port ultra high pressure 1200 bar valve head 1 1535 4857 Stator screws 2 5068 0006 Stator head 3 5068 0008 Rotor seal Vespel 4 1535 4045 Bearing ring 4 3 Figure 44 Column Switching Valve Parts 5067 4117 1290 Infinity Flexible Cube User M anual 169 11 Parts and Materials for Maintenance 7D ha j ac amp Dart Valva Haard GNN har Rina inart os 6 Port Vaive head OUU Dar B10 inert 2 Pos 6 Port Valve Head 600 bar Bio inert yr For bio inert modules use bio inert parts only Item p n 5067 4148 5068 0020 5068 0060 0100 1851 0101 1409 1535 4045 oOo FF WwW N Ff Description Bio inert valve head 2 pos 6 port 600 bar Stator Screws 10 pack Bio inert stator head Stator face ceramic Rotor Seal PEEK Bearing ring Figure 45 Column Switching Valve Parts 170 1290 Infinity Flexible Cube User M anual Parts and Materials for Maintenance 11 2 Pos 10 Port Micro Valve Head 600 bar Item p n Description 5067 4144 Micro Valve Head 2 Position 10 Port 600 bar 1 5068 0054 Stator screws 10 Pk 2 0101 1421 Stator Head 3 0101 1415 Rotor Seal PEEK 4 1535 4045 Bearing ring Figure 46 Column Switching Valve Parts 5067 4144 1290 Infinity Flexible Cube User M anual 171 11 Parts and M ate rials for Maintenance 2 Pos 10 Port Ultra High Pressure Valve Head 1200 bar Item p n 5067 4118 1 5068 0019 2 5068 0011 3 5068 0012 4 1
42. 260 Infinity Bio inert Analytical Fraction Collector G5664A Bio inert Flow Cells Standard flow cell bio inert 10 mm 13 uL 120 bar 12 M Pa forMWD DAD includes Capillary Kit Flow Cells BIO p n G5615 68755 G5615 60022 for Agilent 1260 Infinity Diode Array Detectors DAD G1315C D M ax Light Cartridge Cell Bio inert 10 mm V s 1 0 uL G5615 60018 and M ax Light Cartridge Cell Bio inert 60 mm V s 4 0 uL G5615 60017 for Agilent 1200 Infinity Series Diode Array Detectors DAD G4212A B Bio inert flow cell 8 uL 20 bar pH 1 12 includes Capillary Kit Flow Cells BIO p n G5615 68755 G5615 60005 for Agilent 1260 Infinity Fluorescence Detector FLD G1321B Titanium gold platinum iridium ceramic ruby PTFE PEEK Upstream of sample introduction e Titanium gold PTFE PEEK ceramic Downstream of sample introduction e PEEK ceramic PEEK ceramic PEEK ceramic PTFE PEEK ceramic sapphire PTFE PEEK fused silica PEEK fused silica PTFE 1290 Infinity Flexible Cube User M anual 27 1 Introduction Table1 Bio inert materials used in Agilent 1260 Infinity Systems Module Materials Bio inert heat exchanger G5616 60050 PEEK steel cladded for Agilent 1290 Infinity Thermostatted Column Compartment G1316C Bio inert Valve heads G4235A G5631A G5639A PEEK ceramic Al 03 based Bio inert Connection capillaries Upstream of sample introduction e Titanium Downstream o
43. 535 4045 Description 2 pos 10 port ultra high pressure 1200 bar valve head Stator screws Stator head Rotor seal Vespel Bearing ring Figure 47 Column Switching Valve Parts 5067 4118 172 1290 Infinity Flexible Cube User M anual Parts and Materials for Maintenance 11 8 Pos 9 Port Valve Head 600 bar 8 Pos 9 Port Valve Head 600 bar Item p n 5067 4107 1 1535 4857 2 5068 0001 3 5067 4111 4 1535 4045 2 Description Valve Head 8 Position 9 Port 600 bar Stator screws Stator head Rotor Seal PEEK Bearing ring Figure 48 Column Switching Valve Parts 5067 4107 1290 Infinity Flexible Cube User M anual 173 11 Parts and Materials for Maintenance 8 Pos 9 Port Ultra High Pressure Valve Head 1200 bar 8 Pos 9 Port Ultra High Pressure Valve Head 1200 bar Item p n Description 5067 4121 8 pos 9 port valve head high pressure 1200 bar 1 1535 4857 Stator screws 2 5068 0001 Stator head 3 5068 0002 Rotor seal Vespel 4 1535 4045 Bearing ring A F I Figure 49 Column Switching Valve Parts 5067 4121 174 1290 Infinity Flexible Cube User M anual Parts and Materials for Maintenance 11 Valve Head 4 Column Selector 600 bar Bio inert For bio inert modules use bio inert parts only inert Item p n 5067 4134 1 5068 0059 5068 0044 5068 0093 5068 0045 1535 4045 un FSF WD N Description Bio inert valve head 4 column selector 600 bar Stator screws Bio inert stator head
44. 61 Parts overview 162 Capillaries 163 Accessory Kits 164 Valve Options Overview 166 2 Pos 6 Port Valve Head 600 bar 168 2 Pos 6 Port Ultra High Pressure Valve Head 1200 bar 169 2 Pos 6 Port Valve Head 600 bar Bio inert 170 2 Pos 10 Port Micro Valve Head 600 bar 171 2 Pos 10 Port Ultra High Pressure Valve Head 1200 bar 172 8 Pos 9 Port Valve Head 600 bar 173 8 Pos 9 Port Ultra High Pressure Valve Head 1200 bar 174 Valve Head 4 Column Selector 600 bar Bio inert 175 Valve Head 6 Column Selector 600 bar 176 Valve Head 6 Column Selector 1200 bar 177 12 Pos 13 Port Valve Head 210 bar Bio inert 178 12 Identifying Cables 179 Cable Overview 180 Analog Cables 182 Remote Cables 184 BCD Cables 187 CAN LAN Cables 189 External Contact Cable 190 Agilent Module to PC 191 Agilent 1200 Module to Printer 192 13 Hardware Information 193 Firmware Description 194 Electrical Connections 197 Interfaces 199 Setting the 8 bit Configuration Switch without On board LAN 206 Instrument Layout 212 Early Maintenance Feedback 213 1290 Infinity Flexible Cube User M anual Contents 14 Appendix 215 General Safety Information 216 The Waste Electrical and Electronic Equipment W EEE Directive 2002 96 EC 219 Radio Interference 220 SoundEmission 221 Agilent Technologies on Internet 222 8 1290 Infinity Flexible Cube User M anual 1290 Infinity Flexible Cube User M anual 1 Introduction Features 10 Overv
45. Cube Flexcube has a leakage Error ID 4726 A leak was detected in the module The signals of the two temperature sensors leak sensor and the board mounted temperature compensation sensor are used by the leak sensor algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fitting Ensure all fittings are tight 2 Broken tubing Exchange defective tubing 1290 Infinity Flexible Cube User M anual 115 8 116 Error Information Flexcube fan failed Error ID 4727 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain period of time the error message is generated Probable cause 1 Fan cable disconnected 2 Defective fan 3 Defective main board Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Pump failed at ejecting or at initializing The stepper motor of the flush pump did not reach its end position If the flush pump is pumping against a restriction it will lose steps and thereby loose the position It will then not be able to reach its home position
46. Cube A How to Achieve Lowest Carry Over Calculation of Time 1 Time 1 Sample flush out factor Total flush out volume Flow rate Total flush out volume Injection volume Seat capillary volume Valve volume 94 1290 Infinity Flexible Cube User M anual Optimizing Lab Performance with Flexible Cube 6 How to Achieve Highest Throughput Using the Flexible Cube A high throughput of the system can still be achieved by using the high throughput features of the autosampler Any wash cycle will increase the cycle time of the system The shortest cycle times using the flush function of the Flexible Cube can be achieved when only flushing with solvent 3 set to the chromatographic starting conditions only The volume which has to be flushed can be calculated by adding the volume of the autosampler valve and the volume of the autosampler seat capillary With the known flow rate of the pump in the Flexible Cube the flushing time can be calculated with the desired factor to flush the volume once or multiple times A minimum of 5 seconds is recommended in order to get solvent in the needle seat exchanged For sticky samples or if sample discrimination is a problem it can be necessary to use multiple solvents This will increase the minimum cycle time achievable Typically one strong dissolving solvent and a solvent set to the chromatographic starting conditions is sufficient but in case solubility issues between strong dissolving solvent and
47. Flexible Cube User M anual Appendix 14 WARNING DAU alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circui
48. If the needle seat or rotor seal becomes contaminated it will have to be back flushed by manually changing the flow connections to clean it This is one of the tasks that can be automated using the Flexible Cube module Analytical head Needle Needle seat Gig Flush pump i Injection valve Solvent selection valve Figure 26 Schematic of Autosampler with Flexible Cube Setup 90 1290 Infinity Flexible Cube User Manual Optimizing Lab Performance with Flexible Cube 6 If compounds are analyzed which are highly sticky and cannot be removed completely by an exterior needle wash they will contaminate the injector needle seat To avoid the manual procedure of back flushing the Flexible Cube can be introduced into the LC system The Flexible Cube contains a single piston pump and up to three solvent selection valves This setup allows flushing the needle seat backwards with three different solvents of different strength The flushing procedure starts prior to the injection to remove residues from the last injection while the needle is drawing the sample and then flushed from the outside in the flush port Thereby it is recommended to start with the strong dissolving solvent 1 and if necessary to continue with strong dissolving solvent 2 Prior to injection the seat and seat capillary should be flushed with solvent 3 to set it similar to chromatographic starting conditions To get the most efficient washing
49. a sample clean up set up 1 Analytical column 2 280 mm length column length gt 100 mm from analytical column to detector Not pre swaged on column side 3 340 mm length from autosampler and loading pump 4 150 mm length column length up to 100 mm 280 mm length column length gt 100 mm From column Not pre swaged on column side Pre column 90 mm length capillary to column 700 mm length 0 17 mm ID from analytical pump To waste ola nti Di wm 90 mm length capillary to column Pos 1 Connection between ports 1 6 4 5 2 3 active column 1 left enrichment column Pos 2 Connection between ports 1 2 3 4 5 6 active column 2 right analytical colum 156 1290 Infinity Flexible Cube User M anual Maintenance and Repair 10 Installing the Capillaries Figure 41 Installing the capillaries for alternating column regeneration column of the second position omitted 1290 Infinity Flexible Cube User M anual 157 10 Maintenance and Repair 158 oOo ao nti Ds wo Valve Valve connector 120 mm length 150 mm length column length up to 100 mm 280 mm length column length gt 100 mm From column Not pre swaged on column side 200 mm length to detector 150 mm length column length up to 100 mm 280 mm length column length gt 100 mm From column Not pre swaged on column side Column 90 mm length capillary to column To waste From autosampler 700 mm length 0 17 mm ID from regeneration pump
50. able7 Maintenance Procedures Procedure Typical Frequency Notes Exchange Flush Pump Inlet When leaking Run Flush pump delivery test Valve on page 139 for verification Exchange Flush Pump Outlet When leaking Run Flush pump delivery test Valve on page 141 for verification Exchange Valve Rotor If damaged blocked or leaking Run Pressure Test for Seal on page 143 verification Installing the Capillaries on When new application page 152 requires a change Replacing Valve Heads on If the valve performance page 148 shows indication of leakage or wear Replacing M odule If required Firmware on page 159 Preventive maintenance is usually not necessary only for the rotor seal 1290 Infinity Flexible Cube User M anual 137 10 Maintenance and Repair Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 138 1290 Infinity Flexible Cube User M anual Maintenance and Repair 10 Exchange Flush Pump Inlet Valve When If the Flush pump delivery test fails or if the Inlet valve show signs of malfunction Tools required p n Description 8710 1924 Wrench open 14mm
51. al contains technical reference information about the Agilent 1290 Infinity Flexible Cube G4227A introduction and specifications installation using and optimizing troubleshooting and diagnose maintenance e parts identification hardware information safety and related information 232 1290 Infinity Flexible Cube User M anual
52. all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in this manual For details refer to the Agilent Lab Advisor software help files The Instrument Utilities is a basic version of the Lab Advisor with limited functionality required for installation use and maintenance No advanced repair troubleshooting and monitoring functionality is included 1290 Infinity Flexible Cube User M anual 1290 Infinity Flexible Cube User M anual e0 0 ee 8 7 e Error Information e E e What Are Error Messages 106 e General ErrorMessages 107 Timeout 107 Shutdown 108 Remote Timeout 108 Lost CAN Partner 109 Leak Sensor Short 109 Leak SensorOpen 110 Compensation Sensor Open 110 Compensation SensorShort 111 Fan Failed 111 Leak 112 Open Cover 112 Cover Violation 114 Module ErrorMessages 115 Flexcube has a leakage 115
53. and an error message is generated Probable cause 1 Blocked needle seat 2 Tubing not correctly installed 3 Flexible Cube not configured in software 4 Defective flush pump Suggested actions Replace blocked needle seat Configure tubing to position 4 of injection valve Configure Flexible Cube correctly Please contact your Agilent service representative 1290 Infinity Flexible Cube User M anual Error Information 8 Valve failed to switch or didn t initialize Lost steps of the valve encoder The valve drive has lost its position information and is not able to initialize Probable cause Suggested actions 1 Valve drive mechanically blocked or defect Check installation of valve head e Please contact your Agilent service representative Initialization of Valve Failed Error ID 24000 During the initialization process the motor of the valve drive moves to some special positions depending on the installed valve head A failure in this process means either that the movement couldn t be performed properly or it was not noticed correctly by the sensor Probable cause Suggested actions 1 Mechanical problems Friction too high or e Check valve head for correct installation blockages on the valve drive s motororon Try to identify the source of trouble by the valve head installing a different valve head if possible Contact your Agilent Service representative 2 Defect Sensor on the Valve Drive M otor e Check val
54. ar OR 1 5067 4117 2 pos 6 port ultra high pressure 1200 bar valve head OR 1 5067 4144 Micro Valve Head 2 Position 10 Port 600 bar OR 1 5067 4118 2 pos 10 port ultra high pressure 1200 bar valve head OR 1 5067 4146 Valve head 6 column selector 600 bar OR 1 5067 4142 Valve head 6 column selector 1200 bar OR 1 5067 4148 Bio inert valve head 2 pos 6 port 600 bar OR 1 5067 4134 Bio inert valve head 4 column selector 600 bar OR 1 5067 4159 Bio inert selector valve 12 position 13 port 210 bar WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt Be sure that no solvent can drop out of the solvent connections when removing them from your valve head gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice 148 1290 Infinity Flexible Cube User M anual CAUTION Maintenance and Repair 10 Valve Damage Using a low pressure valve on the high pressure side can damage the valve gt When using multiple column compartments as part of a method development solution make sure that the high pressure valve head is connected to the autosampler and the low pressure valve head is connected to the detector CAUTION
55. ate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as 1290 Infinity Flexible Cube User M anual Hardware Information 13 Table 11 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD RX 2 2 RX TX 3 3 TX DTR 4 4 DTR GND 5 5 GND DSR 6 6 DSR RTS 7 7 RTS CTS 8 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 56 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board 1290 Infinity Flexible Cube User M anual 203 13 Hardware Information APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysi
56. by a metal component representative Leak Sensor Open Error ID 0083 The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the main Please contact your Agilent service board representative 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being Please contact your Agilent service pinched by a metal component representative Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the main board in the module has failed open circuit 110 1290 Infinity Flexible Cube User M anual Error Information 8 The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the main b
57. d M aterial section 1x 8Pos 9Port 1200bar valve head Table9 Agilent Quick Change Valve heads Bio inert G4230A includes 2 x 8Pos 9Port 600bar valve heads G4230B includes 1x 8Pos 9Port 600bar and Kit description Valve head Rotor seal Stator heads 2pos 6port valve Bio inert valve head 2 bio inert G5631A pos 6 port 600 bar 5067 4148 12pos 13port valve Bio inert selector Bio inert rotor seal Bio inert stator head bio inert G4235A valve 12 position 13 and stator face kit 5068 0097 port 210 bar 0101 1288 5067 4159 4 column selector Bio inert valve head4 Bio inert rotor seal Bio inert stator head valve bio inert column selector 600 PEEK 5068 0045 5068 0044 G5639A bar 5067 4134 1 kit with stator face and rotor seal 1290 Infinity Flexible Cube User M anual 167 11 Parts and Materials for Maintenance Dre IA Dart V A ENNA har POS 0 Port Vaive read OUU Da 2 Pos 6 Port Valve Head 600 bar Item p n 5067 4137 1 1535 4857 2 0101 1417 3 0101 1409 4 1535 4045 Description Valve Head 2 Postion 6 Port 600 bar Stator screws 10 Pk Stator head Rotor Seal PEEK Bearing ring Figure 43 Column Switching Valve Parts 5067 4137 168 1290 Infinity Flexible Cube User M anual Parts and Materials for Maintenance 11 Dac C Dart Ilira Hinh Dracciira Vial aan 1900 har Pos 6 Port Ultra High Pressure Valve Head 1200 bar 2 Pos 6 Port Ultra High Pressure Valve Head
58. e substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice 146 1290 Infinity Flexible Cube User M anual Maintenance and Repair 10 1 Use the Hex Key to open and remove the Stator Screws from the Stator Head 2 Carefully disassemble the necessary Valve Head parts to gain access to the one you want to replace While doing so please observe the orientation of the parts 3 Independent of the part you want to replace always inspect all parts for signs of damage 4 Replace the proposed part Always mind the correct orientation of the parts and avoid to touch their surfaces 5 Turn each of the screws an equal amount until they are finger tight then tighten them for another half turn 1290 Infinity Flexible Cube User M anual 147 10 Maintenance and Repair Replacing Valve Heads Several optional valve heads are available which can be installed and exchanged easily Micro valves offer small internal volumes for minimum peak broadening ideal for low flow rates in the nl min and ul min range yy For bio inert modules use bio inert parts only Parts required p n Description 1 5067 4121 8 pos 9 port valve head high pressure 1200 bar OR 1 5067 4107 Valve Head 8 Position 9 Port 600 bar OR 1 5067 4137 Valve Head 2 Postion 6 Port 600 b
59. e the Isolation seal 144 1290 Infinity Flexible Cube User M anual Maintenance and Repair 10 7 Install new rotor seal R CN A 8 Install the stator head and the stator ring 9 With a 9 64 inch hex key alternately tighten the stator screws 10 Reinstall all capillaries to the valve head depending on the type and usage of the valve 11 Close the front cover and prime the flush pump before use to warrant trouble free usage 1290 Infinity Flexible Cube User M anual 145 10 Maintenance and Repair Replacing Parts of the Valve Head inert When Tools required For details about the needed parts and orientation please refer to Parts and Materials for Maintenance on page 161 Disassembling and reassembling the valve head For bio inert modules use bio inert parts only Stator head Scratches and damages on the inner surface blockages Stator face assy When visibly scratched or when the valve performance shows indication of leakage or wear Rotor seal assy After approximately 10000 to 20000 injections or when the valve performance shows indication of leakage or wear Description Hex key Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks gt Be sure that no solvent can drop out of the solvent connections when removing them from your valve head gt When working with thes
60. entifying Cables 12 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n Description G1103 61611 External contact cable Agilent module interface board to general purposes RS 232 cables p n Description G1530 60600 RS 232 cable 2 m RS232 61601 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m 1290 Infinity Flexible Cube User M anual 181 12 Identifying Cables Analog Cables 182 JEIO One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent M odule to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog Agilent M odule to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module G Shield Shield Analog ia Center Center Analo
61. ert tool Hex driver 1 4 slitted Hex key wrench 9 64 inch Wrench open 1 4 5 16 inch Hex key set 1 5mm Pozidriv screwdriver Wrench 4mm both ends open end Wrench open 14mm Hex key 4 mm15 cm long T handle Hex key 1 5 mm straight handle 10 cm Hex Key 9 64 inch 15 cm long T handle Wrench open end 5 16 3 8 inch Hex key 3mm12 cm long Hex key 2 5 mm 15 cm long straight handle Hex key 2 0 mm Screwdriver Torx TX8 Double open end wrench 4 mm 1290 Infinity Flexible Cube User M anual p n 9301 0411 9301 1337 Parts and Materials for Maintenance 11 Description Syringe Plastic Adapter syringe solvent tube with fitting Flexible Cube Accessory Kit The Flexible Cube Accessory Kit G4227 68705 contains some accessories and tools needed for installation and repair of the module p n 0100 1816 0890 2207 5067 4680 5181 1519 9301 1420 G4220 60007 64226 87012 5043 0909 1290 Infinity Flexible Cube User M anual Description Fitting Waste Tube to Purge Valve Tubing Sleeving Flex Tubing Kit 600 mm 130 bar CAN cable Agilent module to module 1 m Solvent bottle transparent Bottle Head Assembly Needle seat Tubing Flex PE 3m 165 11 Parts and Materials for Maintenance Valve Options Overview This overview gives a summary of the main parts and assemblies More details are available with each valve option in this chapter Table8 Agilent Quick Change Valve Heads Kit description Val
62. es 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler Special Interfaces There is no special interface for this module 1290 Infinity Flexible Cube User M anual 205 13 Hardware Information Setting the 8 bit Configuration Switch without On board LAN 206 The 8 bit configuration switch is located at the rear of the module This module does not have its own on board LAN interface It can be controlled through the LAN interface of another module and a CAN connection to that module Figure 57 Configuration switch settings depend on configured mode All modules without on board LAN e default should be ALL DIPS DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no
63. f sample introduction e Agilent uses stainless steel cladded PEEK capillaries which keep the flow path free of steel and provide pressure stability to more than 600 bar 28 To ensure optimum bio compatibility of your Agilent 1260 Infinity Bio inert LC system do not include non inert standard modules or parts to the flow path Do not use any parts that are not labeled as Agilent Bio inert For solvent compatibility of these materials see M aterial Information on page 78 1290 Infinity Flexible Cube User M anual 1290 Infinity Flexible Cube User M anual e e t M o e ee 2 7 e Site Requirements and Specifications e i e j Site Requirements 30 Physical Specifications 33 Performance Specifications 34 This chapter provides information on environmental requirements physical and performance specifications ot Agilent Technologies 29 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 2 on page 33 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING Hazard of electrical shock or damage of your instrumentation
64. filter movements raw data collection and conversion to absorbance 194 1290 Infinity Flexible Cube User M anual Hardware Information 13 Firmware Updates Firmware updates can be done using your user interface PC and Firmware Update Tool with local files on the hard disk Instant Pilot G4208A with files from a USB Flash Disk Agilent Lab Advisor software B 01 03 and above The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315AB for the G1315A B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 102 is revision 1 02 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Main FW update Main System Resident System Resident FW Update Figure 54 Firmware Update M echanism 1290 Infinity Flexible Cube User M anual 195 13 Hardware Information 196 Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A Xx xx
65. formance can be achieved Ateach time the valve is switched twice from mainpass to bypass to mainpass These additional switches must be taken into account when calculating the number of runs that can be executed until the rotor seal of the injection valve needs to be replaced EM F The rotor seal of the Autosampler has a lifetime of around 30000 switches When other measures have failed to eliminate carryover it might be that analyte is sticking inside the injector valve The injector valve can be set to make additional switching movements to clean out the flow path in the valve if problems occur here with carryover Bore of the stator Groove of the rotor Figure 27 The Problem Zone of the Injection Valve If the problem compounds need a high percentage of organic phase for elution it is recommended to switch the injection valve at the high percentage of organic phase after the last peak has eluted It is also recommended to switch the injection valve again after the initial conditions for the mobile phase have stabilized This ensures that the bypass groove in the rotor seal of the valve contains the gradient start conditions which is especially important when using short narrow bore columns The Injection Valve Cleaning section allows you to specify the valve switching times anytime appropriate 92 1290 Infinity Flexible Cube User M anual Optimizing Lab Performance with Flexible Cube 6 Organic Phase Inject
66. g 6 Click OK 126 1290 Infinity Flexible Cube User M anual TestFunctions 9 Flush Pump Delivery Test During the test the flush pump delivers at 3 mL min against the blocked line to the G4220A Binary pump The test is looking for the maximum pressure the flush pump can generate before it errors out The pressure increase can be observed in the signals tab a 2p ten Desenpton Qed eeta tne think pump n capable of generating tree hredi enough rassure Module Approx Tino Statur Munning TIT Prevare ber 0 Figure 34 Flush pump delivery test Signals screen 1290 Infinity Flexible Cube User M anual 127 9 Test Functions Flush Pump Delivery Test The maximum pressure the flush pump should be able to generate during the test should be above 40 bar magnast Results a opter GLAC Figure 35 Flush pump delivery test Limits 128 1290 Infinity Flexible Cube User M anual TestFunctions 9 Flush Pump Delivery Test At the end of the Flush pump delivery test the capillary connection should be restored The Flexible Cube to Autosampler capillary should be removed from the injection valve port 6 and reconnected to the port 4 Please don t Forget to reconnect your system capillaries 7 After reconnection of your system capillaries click OK 1290 Infinity Flexible Cube User M anual 129 9 Test Functions Flush Pump Delivery Test Descepbhon Checks rhehes the Rush pane n capable of geneiatin
67. g 1290 Infinity Flexible Cube User M anual Agilent M odule to General Purpose Identifying Cables 12 p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog 3 Red Analog _ ee 1290 Infinity Flexible Cube User M anual 183 12 Identifying Cables Remote Cables 184 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent M odule to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent SignalName Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low A 3 3 Gray Start Low 2 NC 4 Blue Shut down Low S NC 5 Pink Not w connected o NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent M odule to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1290 Infinity Flexible Cube User M anual Identifying Cables 12 Agilent M odule to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent SignalName Active module TTL 9 1 White D
68. g oreaz pacman Figure 36 Flush pump delivery test Test Passed 130 1290 Infinity Flexible Cube User M anual 1290 Infinity Flexible Cube User M anual o est Failed e o 70 Probable cause 1 Flush pump solvent inlet filter blocked 2 No solvent in flush pump bottle 3 Solvent lines not configured correctly 4 Defective flush pump Suggested actions Change solvent inlet filters Check for sufficient solvent amount available Check for correct setup of solvent bottles Replace the flush pump Ee Agilent Technologies 131 9 Test Functions Pressure Test For running a Pressure Test please refer to the corresponding pump manual The Pressure Test may be used for testing the tightness of a valve installed in the TCC or Flex Cube CAUTION Wrong use of Pressure Test may damage valve The current implementation of the Pressure Test automatically uses the maximum pressure generated by the pump used in the system gt Do not use the test for modules having a lower maximum pressure than the pump as this will damage the valve For example do not use 400 bar valve in a TCC or Flex Cube in combination with a 600 bar pump e g G1312B Binary Pump 132 1290 Infinity Flexible Cube User M anual Maintenance andRepair 10 Pressure Test 10 Maintenance and Repair Introduction to Maintenance 134 Warnings and Cautions 135 Overview of Maintenance 137 Cleaning the Module 138 Exchange Flush Pump Inlet
69. he Autosampler flow path 1290 Infinity Flexible Cube User Manual 15 1 Introduction Flexible Cube Technical Capability 4 Flush with dissolving solvent 2 B2 i Analytical head i Needle seat D er a Flush pump F at Injection valve Solvent selection valve AN VN Figure 4 Flush with dissolving solvent 2 B2 If this is not sufficient to reduce the carry over a dissolving solvent 2 may be used with either different modifiers and or a really strong cleaning solvent 16 1290 Infinity Flexible Cube User Manual NOTE Introduction 1 Flexible Cube Technical Capability 5 Flush with Starting condition A1 Analytical head Needle Needle seat LS p Flush pump D E Injection valve Solvent selection valve Di Figure 5 Flush with Starting condition A1 Solvent 3 is always set for the final flush and should be the starting condition of the gradient or lower solution strength as this solvent will be in the needle seat and seat capillary at the point of injection and might lead to reduced resolution if stronger elution strength than mobile phase Any flushing solvent used must be miscible with previous solvent If this is not the case it can result in precipitations blockages or reduced performance of the instrument 1290 Infinity Flexible Cube User M anual 17 1 Introduction Flexible Cube Technical Capability 6 Switch back
70. higher noise levels since the detector is the last module in the flow path this problem is less common How to Prevent and or Reduce the Algae Problem Always use freshly prepared solvents especially use demineralized water which was filtered through about 0 2 um filters e Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle Solvent bottle amber 9301 1450 supplied with the instrument for your aqueous mobile phase 1290 Infinity Flexible Cube User M anual 83 5 Using the Module Algae Growth in HPLC Systems If possible add a few mg l sodium azide or a few percent organic solvent to the aqueous mobile phase 84 1290 Infinity Flexible Cube User M anual 1290 Infinity Flexible Cube User M anual 6 Optimizing Lab Performance with Flexible Cube Preparing the Autosampler and Flexible Cube 86 How to Achieve Lowest Carry Over 87 Internal Needle Wash 88 External Needle Wash 89 Needle Seat Back Flush 90 Injection Valve Rinse 92 How to Achieve Highest Throughput Using the Flexible Cube 95 How to Achieve Higher Injection Volumes 96 How to Achieve High Throughput 97 This chapter provides information on how to optimize the Autosampler and the Flexible Cube for a minimum carry over and fastest cycle times ot Agilent Technologies 85 6 Optimizing Lab Performance with Flexible Cube Preparing the Autosampler and Flexible Cube
71. iew ofthe Module 11 Flexible Cube Technical Capability 12 Valve solution Valve technique 19 Dual and Multi Column Selection 19 Sample Enrichment and Sample Cleanup 21 Alternating Column Regeneration 24 Solvent Selection 26 Bio inert Materials 27 This chapter gives an introduction to the module instrument overview and internal connectors a Agilent Technologies 1 Introduction Features 10 The Agilent 1290 Infinity Flexible Cube introduces an unprecedented flexibility to the Agilent 1290 Infinity System It includes options to reduce carry over and optionally you can add one or two valve pods to be able to use the G4226A HiP Autosampler as a multipurpose valve box The G4227A Flexible Cube is capable of reducing carry over to a minimum and maintains that level between the maintenance intervals of the Autosampler For specifications see Performance Specifications on page 34 This 1290 Infinity Flexible Cube has been introduced as part of the Agilent 1290 Infinity Liquid Chromatograph 1290 Infinity Flexible Cube User M anual Introduction 1 Overview of the Module The Agilent 1290 Infinity Flexible Cube comprises a flush pump that can deliver flow between 1 and 4 mL min from 3 different solvent bottles The system also includes slots so you can add one or two valve drives optionally The standard version of the Agilent 1290 Infinity Flexible Cube supports the needle seat back flush and one valve drive Another
72. igital ground NC 2 Brown Prepare run Low 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected aa NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected 185 1290 Infinity Flexible Cube User M anual 12 Identifying Cables Agilent M odule to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 Pin Agilent SignalName Active A D module TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low Agilent M odule to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL White 1 Digital ground O go Brown 2 Prepare run Low 00 DE KEY Gray 3 Start Low oo Hoe Blue 4 Shut down Low oe AG on Pink 5 Not oo connected s 15 Q Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 186 1290 Infinity Flexible Cube User M anual Identifying Cables 12 BCD Cables a O O A g 4 yE ee ee VENO 0BBGOOOF ANS One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrumen
73. ion Injection a Sample flush out time Time 5 amp 5 Times 1 to 4 are the times when the valve switches to bypass for time 1 or to mainpass and bypass for times 2 3 and 4 The times must be specified in ascending order You can also switch the times to off Between the first and second and second and third valve switches a rinse is executed using the rinse volumes specified in the Injector Cleaning section Injection Valve M ovements You specify the number of times that the injection valve switches from mainpass to bypass at times 2 3 and 4 in the field The maximum value is 2 default is 1 Time1 Valve switch directly after sample has been flushed out at high water might be useful if sample is highly soluble in water Time 2 Valve switch at high organic phase to effectively remove sample from injection valve grooves while avoiding sample retardation on the column Time 3 Valve switch at low organic phase to effectively remove organic solvent from injection valve groove Time 4 Used to repeat valve switch 1 2 or 3 in the case of extremely sticky sample or solvents At each time the valve is switched twice from mainpass to bypass to mainpass These additional switches must be taken into account when calculating the number of runs that can be executed until the rotor seal of the injection valve needs to be replaced EM F 1290 Infinity Flexible Cube User Manual 93 6 Optimizing Lab Performance with Flexible
74. iquid from a leak has been dried Errors for presumably single time events can be recovered by switching on the system in the user interface Special handling is done in case of a leak As a leak is a potential safety issue and may have occurred at a different module from where it has been observed a leak always causes a shutdown of all modules even outside a method run In all cases error propagation is done via the CAN bus or via an APG remote cable see documentation for the APG interface 1290 Infinity Flexible Cube User M anual Error Information 8 General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 A not ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required 1290 Infinity Flexible Cube User M anual 107 8 108 Error Information Shutdown Error ID 0063 An external instrument has generated a shutdown signal on the remote line The module c
75. is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause 1 Loose fittings 2 Broken capillary Open Cover Error ID 0205 The top foam has been removed Suggested actions Ensure all fittings are tight Exchange defective capillaries 1290 Infinity Flexible Cube User M anual Error Information 8 aneral Error Me jeneral Error Me Probable cause Suggested actions 1 Foam not activating the sensor Please contact your Agilent service representative 2 Dirty or defective sensor Please contact your Agilent service representative 1290 Infinity Flexible Cube User M anual 113 8 Error Information Cover Violation Error ID 7461 The top foam has been removed The sensor on the main board detects when the top foam is in place If the foam is removed while the lamps are on or if an attempt is made to switch on for example the lamps with the foam removed the lamps are switched off and the error message is generated Probable cause Suggested actions 1 The top foam was removed during Please contact your Agilent service operation representative 2 Foam not activating the sensor Please contact your Agilent service representative 114 1290 Infinity Flexible Cube User M anual Error Information 8 Module Error Messages These error messages are specific for the Flexible
76. le Cube ie Sequence DEF_LCS 72 A right click into the Active Area will open a menu to Show the Control User Interface special module settings e Show the Method User interface same as via menu Instrument Setup G4226A And Flexible Cube specific items e Autoclean Purge Prime 1290 Infinity Flexible Cube User M anual UsingtheModule 5 Setting up the Flexible Cube with Agilent ChemStation Autoclean TEETE oog The Autoclean function allows for a complete cleaning of UERN the Autosampler This process has been automated for N ease of use and can be configured according to needs and l setup Needle Wash Activate needle wash and setting of Solvent 1 A2 the wash time Solvent 2 82 Purge Configure which solvents to use for needle seat back flushing and with what flowrate for how Start cond 41 long Flow 3v ml min e Injection valve Can be selected for cleaning of the Dui E injection valve This will switch the injection valve s ry sec between mainpass and bypass a number of times Injection Valve Switch injection valve Stat Cancel Help Prime Priming can be used when the flush lines have run dry or flush solvents have been changed This feature primes the _ flush solvent inlet lines with fresh solvent Clean wash port V It can be configured what solvent lines to prime and additionally the needle wash port can also be primed with Solvent 1 A2 solvent Solvent 2
77. le under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm Slowly to room temperature to avoid condensation 32 1290 Infinity Flexible Cube User M anual Physical Specifications Site Requirements and Specifications 2 Table 2 Physical Specifications Type Specification Comments Weight 8 2 kg 18 1 Ibs Dimensions 345 x 435 x 140 mm height x width x depth 13 5 x 17 x 5 5 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 80 VA 45W 154BTU Maximum Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC CSA UL 4 55 C 39 131 F 40 70 C 4 158 F lt 95 r h at 40 C 104 F Up to 2000 m 6562 ft Up to 4600 m 15091 ft Installation category Il Pollution degree 2 Non condensing For storing the module For indoor use only 1290 Infinity Flexible Cube User M anual 33 2 Performance Specifications 34 Site Requirements and Specifications Table3 Performance Specifications Type Specification Comment Injection range Precision Accuracy Sampler pressure range Flush types available Pressure range of flush pump Flow rate of flush pump Number of solvents Samp
78. le viscosity range 0 1 20 uL 0 1 40 uL 0 1 100 uL in 0 1 uL increments Precision of G4226A sampler will not be influenced Accuracy of G4226A sampler will not be influenced Up to 1200 bar Needle seat back flushing Needle wash intern Needle wash extern Up to 50 bar Up to 4 mL min Up to 3 solvents 0 2 5cp With 20 mL loop installed With 40 mL loop installed With 100 mL loop installed as provided by G4226A sampler by G4227A Flexible Cube by G4226A sampler by G4226A sampler 1290 Infinity Flexible Cube User M anual Table 3 Type Site Requirements and Specifications Performance Specifications Specification 2 Comment Flush time Carry over Control and data evaluation Local control Communications Flush time depends on method parameters used e g number of solvents and selected flush duration Flushing is done in parallel to running analysis Needle seat flush time to flush with 1 mL of solvent at maximum flow including solvent exchange typically requires 38 s Typically lt 0 001 10 ppm Agilent ChemStation for LC EZChrom Elite M asshunter Agilent Instant Pilot G4208A Controller area network CAN RS 232C APG Remote ready start stop and shut down signals optional four external contact closures and BCD vial number output For measurement conditions see 1 2 3 4 OpenLAB CDS ChemStation Edition C 01 04 OpenLAB CDS EZChrom Editio
79. me of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module WARNING Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides 1290 Infinity Flexible Cube User M anual 135 10 Maintenance and Repair Warnings and Cautions CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 136 1290 Infinity Flexible Cube User M anual Maintenance and Repair 10 Overview of Maintenance The following pages describe maintenance procedures simple repairs that can be done without opening the main cover T
80. ment while the covers are removed 1 Place the Flexible Cube in the stack see Optimizing the Stack Configuration on page 42 2 Ensure the line power switch on the front of the module is OFF switch stands out 1290 Infinity Flexible Cube User Manual 49 3 Installing the Module Installing the Module 3 Connect the power cable to the power connector at the rear of the module SE Agile Techeatopies Procect No GIA UL Sossi No OESrextt0 TT VUUeEUEUYUYY Figure 19 Module rear view 4 Connect the CAN cable to other modules 5 Turn on power by pushing the button at the lower left hand side of the module The status LED should be green NOTE The module is turned on when the line power switch is pressed and the green indicator lamp is illuminated The module is turned off when the line power switch is protruding and the green light is off 50 1290 Infinity Flexible Cube User M anual Installing the Module 3 Flow Connections to the M odule for Lowest Carry Over Parts required Hardware required Software required Preparations p n Description G4227 68705 Flexible Cube Accessory Kit System Data System and or Instant Pilot G4208 with the appropriate revision see Performance Specifications on page 34 Flexible Cube is installed in system Module is partially energized when switched off as long as the power cord is plugged in Repair work
81. mption 33 powercords 31 power supply indicator 101 powerswitch 49 R remote cable 184 repairs cautions and warnings 135 overview 137 replacing firmware 159 159 replace valve heads 148 rinse injection valve 92 RS 232C cable 191 communication settings 208 S safety class 217 Safety general information 216 standards 33 symbols 216 shutdown 108 site requirements 29 powercords 31 special interfaces 205 Special settings boot resident 210 forced cold start 210 specification 228 1290 Infinity Flexible Cube User M anual physical 33 Specifications 29 stack configuration two stack standard 45 two stack thermostatted 47 two stack 45 Status indicator 102 system setup and installation optimizing stack configuration 42 system tool kit 164 T technical capability flexible cube 12 temperature sensor 112 test functions 100 throughput highest 95 timeout 107 troubleshooting error messages 100 106 status indicators 100 101 U unpacking 40 user interfaces 103 user interface flexible cube 65 V valve dummy remove 53 valve heads replace 148 valve head install 54 valve rotor seal exchange 143 voltage range 33 1290 Infinity Flexible Cube User M anual Appendix 14 229 14 Appendix Index WwW warnings and cautions weight 33 230 135 1290 Infinity Flexible Cube User M anual Appendix 14 Index 1290 Infinity Flexible Cube User M anual 231 14 Appendix In This Book This manu
82. n A 04 04 Masshunter B 05 01 TOF amp Q TOF M asshunter B 06 00 QQQ M odules require Firmware Set A B C 06 5x or higher and LC CE Driver Version A 02 06 B 02 08 or later 1290 Infinity Flexible Cube User Manual 35 2 Site Requirements and Specifications Table 3 Type Performance Specifications Specification Comment Safety and maintenance GLP features Housing Extensive diagnostics error detection and display through Control M odule and ChemStation leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas Early maintenance feedback EM F for continuous tracking of instrument usage with user settable limits and feedback messages Electronic records of maintenance and errors All materials recyclable 1 Chromatographic conditions Column Agilent ZORBAX SB C18 2 1 x 50 mm 1 8 um 827700 902 mobile phase A 0 1 TFA in water B 0 1 TFA in acetonitrile isocratic B 35 flow rate 0 5 mL min temperature 30 C solvent A 15s Cleaning procedure Wash solution 2 min with water 0 1 TFA at 4 mL min needle wash with UV detection Sample 1200 ng ul chlorhexidine dissolved in mobile phase A 1 uL injected and measured on G4212A DAD 10 mm cell Wavelength 257 nm 4 ref 360 nm 16 slit 4 nm 10 Hz MS detection Sample 50 ng pl chlorhexidine dissolved in mobile phase A 1
83. n or contact Agilent Disclaimer Subsequent data were collected from external resources and are meant as a reference Agilent cannot guarantee the correctness and completeness of such information Data is based on compatibility libraries which are not specific for estimating the long term life time under specific but highly variable conditions of UHPLC systems solvents solvent mixtures and samples Information can also not be generalized due to catalytic effects of impurities like metal ions complexing agents oxygen etc Apart from pure chemical corrosion other effects like electro corrosion electrostatic charging especially for non conductive organic solvents swelling of polymer parts etc need to be considered Most data available refers to room temperature typically 20 25 C 68 77 F If corrosion is possible it usually accelerates at higher temperatures If in doubt please consult technical literature on chemical compatibility of materials 1290 Infinity Flexible Cube User M anual UsingtheModule 5 PEEK PEEK Polyether Ether Ketones combines excellent properties with regard to chemical resistance mechanical and thermal stability It is stable in a pH range of 1 to 12 5 and inert to many common solvents There are several known incompatibilities with chemicals such as chloroform methylene chloride THF DMSO gt 1 strong acids nitric acid gt 10 sulphuric acid gt 10 trichloroacetic acid sulfonic acid
84. n which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations the plastic layers help cushion the electronic and mechanical parts from physical shock and the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 212 1290 Infinity Flexible Cube User M anual Hardware Information 13 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EM F Counters EMF co
85. neration ee E E TS ST Wich alternating Anasii Regane Analysis 3 TOgmnerion N Analysis2 Regene Figure 11 Alternate Column Regeneration Time Scheme Solvent Selection The 12pos 13port valve G4235A can be used for solvent selection flow rate lt 10 mL min as illustrated in Figure 12 on page 26 It offers automated access to 12 different eluents up to 12 bottles Pump Figure 12 Solvent Selection Schematic Setup 1290 Infinity Flexible Cube User Manual Bio inert Materials Introduction 1 For the Agilent 1260 Infinity Bio inert LC system Agilent Technologies uses highest quality materials in the flow path also referred to as wetted parts which are widely accepted by life scientists as they are known for optimum inertness to biological samples and ensure best compatibility with common samples and solvents over a wide pH range Explicitly the complete flow path is free of stainless steel and free of other alloys containing metals such as iron nickel cobalt chromium molybdenum or copper which can interfere with biological samples The flow downstream of the sample introduction contains no metals whatsoever Table1 Bio inert materials used in Agilent 1260 Infinity Systems Module Materials Agilent 1260 Infinity Bio inert Quaternary Pump G5611A Agilent 1260 Infinity Bio inert High Performance Autosampler G5667A Agilent 1260 Infinity Bio inert M anual Injector G5628A Agilent 1
86. ngs for RS 232C Communication without on board LAN Mode Select 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up Table 15 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table 16 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 7 Bit Communication 1 8 Bit Communication 1290 Infinity Flexible Cube User M anual Hardware Information 13 Table 17 Parity Settings without on board LAN Switches 7 8 0 0 0 1 1 1 Parity No Parity Odd Parity Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity 1290 Infinity Flexible Cube User M anual 209 13 Hardware Information Special Settings The special settings are required for specific actions normally in a service case Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode I
87. ns to the Module for Lowest Carry Over 51 Installing the Valve Heads 53 LAN Configuration 59 LAN Configuration 60 Using the Module 61 Instrument Configuration 62 Setting up the Flexible Cube with Agilent ChemStation 64 Solvent Information 78 Algae Growth in HPLC Systems 83 1290 Infinity Flexible Cube User M anual Contents Optimizing Lab Performance with Flexible Cube 85 Preparing the Autosampler and Flexible Cube 86 How to Achieve Lowest Carry Over 87 How to Achieve Highest Throughput Using the Flexible Cube 95 How to Achieve Higher Injection Volumes 96 How to Achieve High Throughput 97 Troubleshooting and Diagnostics 99 Overview of the M odule s Indicators and Test Functions 100 Status Indicators 101 User Interfaces 103 Agilent Lab Advisor Software 104 Error Information 105 What Are Error Messages 106 General Error Messages 107 M odule Error Messages 115 Test Functions 121 Introduction 122 Flush Pump Delivery Test 123 Pressure Test 132 10 Maintenance and Repair 133 Introduction to Maintenance 134 Warnings and Cautions 135 Overview of Maintenance 137 Cleaning the Module 138 Exchange Flush Pump Inlet Valve 139 Exchange Flush Pump Outlet Valve 141 Exchange Valve Rotor Seal 143 Replacing Parts of the Valve Head 146 Replacing Valve Heads 148 Installing the Capillaries 152 Replacing M odule Firmware 159 1290 Infinity Flexible Cube User M anual Contents 11 Parts and Materials for Maintenance 1
88. oard in the module has failed short circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated 1290 Infinity Flexible Cube User M anual 111 8 112 Error Information Leak Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause 1 Fan cable disconnected 2 Defective fan 3 Defective main board Error ID 0064 A leak was detected in the module Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak
89. olume External contacts board installed use BCD port for Locatior Binary Output BCD port output format BCD Binary Thermostat installed V Flexible Cube installed Rinse valve installed Rinse valve enabled Define Wellplates 1290 Infinity Flexible Cube User Manual Options Lists installed options for the G4226A Autosampler Flexible Cube installed If the Flexible Cube option is checked the Flexible Cube cleaning features becomes activated in the Method and Configuration screens 5 77 5 Using the Module Solvent Information Introduction 78 Observe the following recommendations on the use of solvents Follow recommendations for avoiding the growth of algae see Algae Growth in HPLC Systems on page 83 Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters Avoid or minimize the use of solvents that may corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Material Information Materials in the flow path are carefully selected based on Agilent s experiences in developing highest quality instruments for HPLC analysis over several decades These materials exhibit excellent robustness under typical HPLC conditions For any special conditions please consult the material information sectio
90. olvents There are no documented incompatibilities for HPLC applications Fused silica and Quartz Si0 Fused silica is used in 1290 Infinity Flow Cells and capillaries Quartz is used for classical flow cell windows It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides It is corroded by strong bases and should not be used above pH 12 at room temperature The corrosion of flow cell windows can negatively affect measurement results For a pH greater than 12 the use of flow cells with sapphire windows is recommended Gold Gold is inert to all common HPLC solvents acids and bases within the specified pH range It can be corroded by complexing cyanides and concentrated acids like aqua regia Zirconium Oxide Zr0 Zirconium Oxide is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications 1290 Infinity Flexible Cube User Manual 81 5 82 Using the Module Platinum Iridium Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fluorinated polymers PTFE PFA FEP FFKM Fluorinated polymers like PTFE polytetrafluorethen PFA perfluoroalkoxy and FEP fluorinated ethylene propylene are inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications FFKM is perflu
91. on Switches the valve to the next position Decrease valve position Switches the valve to the previous position Use valve position Switches the valve to a position other than the current one Set the valve position by clicking the down arrow and selecting a position from the drop down list Right valve change position see above Pump volume At the specified time the pump delivers the specified volume of solvent Pumpfor time At the specified time the pump is switched on for the specified time Execute Pump Command Execute pump command x _ C Pump a specified volume Flow 3 0 mUmin Duration i s Volume mL Ok Cancel Help The Execute pump command dialog box allows you to set up and start the operation of the pump for a specified solvent volume or a specified time You can select from None No pump command is executed Pump fora specified time The pump delivers the specified flow rate for the specified time Pumpa specified volume The pump delivers the specified volume of solvent at the specified flow rate Flow Enter the required solvent flow rate in mL min in this field The Flow field is available only when Pump fora specified time or Pumpa specified volume is selected 1290 Infinity Flexible Cube User M anual UsingtheModule 5 Duration Enter the required duration of pumping in seconds in this field The Duration field is available only when Pump fora specified
92. ontinually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system Remote Timeout Error ID 0070 Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause 1 Not ready condition in one of the instruments connected to the remote line Suggested actions Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis 1290 Infinity Flexible Cube User M anual Error Information 8 Probable cause Suggested actions 2 Defective remote cable Exchange the remote cable 3 Defective components in the instr
93. or green yellow red w DK PalisatTechociogioe 1200 innty BJ n y Line power switch with green light Figure 30 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 1290 Infinity Flexible Cube User M anual 101 7 102 Troubleshooting and Diagnostics M odule Status Indicator The module status indicator indicates one of six possible module conditions When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is in a not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e a red LED may indicate a p
94. orinated rubber which is also resistant to most chemicals As an elastomer it may swell in some organic solvents like halogenated hydrocarbons Sapphire Ruby and Al 03 based ceramics Sapphire ruby and ceramics based on aluminum oxide AlgOg are inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications 1290 Infinity Flexible Cube User M anual UsingtheModule 5 Algae Growth in HPLC Systems The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems Algae grow in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental Problems Associated With Algae Algae deposit and grow everywhere within the HPLC system causing Blocked solvent filters or deposits on inlet or outlet valves resulting in unstable flow composition or gradient problems or a complete failure of the pump Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure PTFE frits blockage leading to increased system pressure Column filters to plug giving high system pressure Flow cell windows of detectors to become dirty resulting in
95. parity for RS 232 DIP 1 DOWN and DIP 2 UP allows special RS 232 settings for boot test modes DIPS 1 2 must be UP plus required mode For normal operation use the default best settings Switch settings provide configuration parameters for serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN 1290 Infinity Flexible Cube User M anual Hardware Information 13 The following tables represent the configuration switch settings for the modules without on board LAN only Table 13 8 bit Configuration Switch without on board LAN Mode Select RS 232C Reserved TEST BOOT 1 0 3 4 Baudrate RSVD SYS 5 Data Bits Reserved RSVD Parity RSVD FC The LAN settings are done on the LAN Interface Card G1369B C Refer to the documentation provided with the card 1290 Infinity Flexible Cube User M anual 207 13 Hardware Information 208 Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in order to store the values in the non volatile memory Table 14 Communication Setti
96. patible with some mineral acids For more information see Sovent Information Incompatible with concentrated mineral acids compatible with pH less than 10 compatible with most organics may expe rience swelling with some organics containing functional groups such as nitro benzene at elevated temperatures 3 pre column volume G4234A B 0 51 uL G5639A 0 81 uL post column volume G4234A B 1 28 uL G5639A 1 33 uL 1290 Infinity Flexible Cube User M anual 37 2 Site Requirements and Specifications Performance Specifications 38 1290 Infinity Flexible Cube User M anual 1290 Infinity Flexible Cube User M anual 3 Installing the Module Unpacking the Module 40 Optimizing the Stack Configuration 42 One Stack Configuration 43 Supported Two Stack Configurations 45 Installing the Module 49 Flow Connections to the Module for Lowest Carry Over 51 Installing the Valve Heads 53 This chapter gives information about the preferred stack setup for your system and the installation of your Flexible Cube aa Agilent Technologies 39 3 Installing the Module Unpacking the M odule Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module In
97. port valve 0100 1259 Plastic fittings 0890 1713 Waste tubing 5067 4647 SST Capillary 340 x 0 12 mm ps ps 1sh 1xlg 5067 4648 SST Capillary 700 x 0 17 mm ps ps 1sh 1xlg 5067 4649 SST Capillary 90 x 0 12 mm ps ps Ish 1xlg 5067 4650 SST Capillary 150 x 0 12 mm ns ps 1lg 1xlg 5067 4651 SST Capillary 280 x 0 12 mm ns ps 1lg 1xlg 5067 4652 SST Capillary 120 x 0 12 mm ps ps 1xlg 1xlg 5067 4653 SST Capillary 200 x 0 12 mm ps ps 1sh 1xlg Identify the required capillaries for your set up 1290 Infinity Flexible Cube User M anual Maintenance and Repair 10 Installing the Capillaries NOTE Use outmost care to avoid any void volumes caused by poor connections 1 Install the capillaries depending on your application Following configurations are available for listed applications Please choose your appropriate configuration from this list Figure 38 Installing the capillaries for a dual column selection set up column of the second position omitted 1290 Infinity Flexible Cube User M anual 153 10 Maintenance and Repair 1 150 mm length column length up to 100 mm 280 mm length column length gt 100 mm From column Not pre swaged on column side 2 200 mm length to detector 3 150 mm length column length up to 100 mm 280 mm length column length gt 100 mm From column Not pre swaged on column side 90 mm length capillary Column 90 mm length capillary y OD oO A 90 mm length capillary to column Pos 1
98. r especially when toxic or hazardous solvents are used 1290 Infinity Flexible Cube User M anual The Waste Electrical and Electronic Equipment W EEE Directive 2002 96 EC The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex this product is classed as a Monitoring and Control instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1290 Infinity Flexible Cube User M anual 219 Radio Interference Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and M easurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating condi
99. roblem of a different module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of main firmware A fast blinking indicator indicates that the module is in a low level error mode In such a case try to re boot the module or try a cold start see Special Settings on page 210 Then try a firmware update see Replacing Module Firmware on page 159 If this does not help a main board replacement is required 1290 Infinity Flexible Cube User M anual Troubleshooting and Diagnostics 7 User Interfaces Depending on the user interface the available tests and the screens reports may vary see chapter Test Functions and Calibrations Preferred tool should be the Agilent Lab Advisor software see Agilent Lab Advisor Software on page 104 The Agilent ChemStation B 04 02 and above may not include any maintenance test functions Screenshots used within these procedures are based on the Agilent Lab Advisor software 1290 Infinity Flexible Cube User M anual 103 7 Troubleshooting and Diagnostics Agilent Lab Advisor Software 104 The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or
100. rroded by acids below pH 2 3 It can also corrode in following solvents e Solutions of alkali halides their respective acids for example lithium iodide potassium chloride and so on and aqueous solutions of halogens High concentrations of inorganic acids like nitric acid sulfuric acid and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2 CHCl O gt 2 COCl 2 HCI 1290 Infinity Flexible Cube User Manual UsingtheModule 5 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Diamond Like Carbon DLC Diamond Like Carbon is inert to almost all common acids bases and s
101. rt Autosampler G4226A ALS 2 Yes No Yes No Yes Yes CAN DC OUT for CAN Slaves Yes CAN DC OUT for CAN Slaves Yes THERM OSTAT for G1330B Yes THERM OSTAT for G1330B CAN DC OUT for CAN Slaves Yes 1290 Infinity Flexible Cube User M anual 199 13 Hardware Information Table 10 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes G4212A B DAD 2 No Yes Yes 1 Yes G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 2 Yes G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No 1 G1316A C TCC 2 No No Yes No Yes G1322A DEG No No No No No Yes AUX G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes G4227A Flex Cube 2 No No No No No CAN DC OUT for CAN slaves 1 G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERM OSTAT for G1330A B NOT USED 1 Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or with ad ditional G1369C LAN Card 200 1290 Infinity Flexible Cube User M anual Hardware Information 13 The detector DAD M WD FLD VW D RID is the preferred access point for control via LAN The inter module communication
102. rt 1 gt 2 Pump On Flow 0 00 mL min Solvent A A1 Water Solvent B B1 Bridge Figure22 ChemStation M ethod amp Run Control Flex Cube GUI 1290 Infinity Flexible Cube User M anual UsingtheModule 5 Setting up the Flexible Cube with Agilent ChemStation 1290 ALS Ide M Injection Volume 0 0 pl Wash Cycle Idle Figure 23 ChemStation Method amp Run Control Autosampler GUI The Flexible Cube User Interface A right click in the Active Area of the Flexible Cube will open a menu e show the Control user interface show the Method user interface Error Method Identify Device Switch Pump On Execute Pump Command e Left Valve Position Cluster e Right Valve Position Cluster 1290 Infinity Flexible Cube User Manual 65 5 Using the Module Control Settings 101 x Pump Switch Pump On C Off V Automatic initialization on first command Left Valve Pressure Limit Cluster C None Use Selected Autodetect Right Valve Pressure Limit Cluster C None C Use Selected Autodetect Illumination On O Off Ok Cancel Help The Pump section enables you to switch the pump On or Off Automatic initialization on firstcommand Mark this check box to ensure that the pump is initialized automatically when it is switched on Left valve Pressure Limit Cluster Use this section to specify how the pressure limit for the cluster is to be determined None No cluster has been
103. s 7 Troubleshooting and Diagnostics This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces 1290 Infinity Flexible Cube User Manual 3 In This Guide 8 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how to recover from error conditions 9 Test Functions This chapter describes the tests for the module 10 Maintenance and Repair This chapter describes the maintenance of the module 11 Parts and Materials for Maintenance This chapter provides information on parts and material required for the module 12 Identifying Cables This chapter provides information on cables used with the 1290 series of HPLC modules 13 Hardware Information This chapter describes the module in more detail on hardware and electronics 14 Appendix This chapter provides addition information on safety legal and web 1290 Infinity Flexible Cube User M anual Contents Contents Introduction 9 Features 10 Overview of the Module 11 Flexible Cube Technical Capability 12 Valve solution Valve technique 19 Bio inert Materials 27 Site Requirements and Specifications 29 Site Requirements 30 Physical Specifications 33 Performance Specifications 34 Installing the Module 39 Unpacking the Module 40 Optimizing the Stack Configuration 42 Installing the Module 49 Flow Connectio
104. s halogenes or aequous halogene solutions phenol and derivatives cresols salicylic acid etc When used above room temperature PEEK is sensitive to bases and various organic solvents which can cause it to swell Polyimide Agilent uses semi crystalline polyimide for rotor seals in valves and needle seats in autosamplers One supplier of polyimide is DuPont which brands polyimide as Vespel which is also used by Agilent Polyimide is stable in a pH range between 1 and 10 and in most organic solvents It is incompatible with concentrated mineral acids e g sulphuric acid glacial acetic acid DMSO and THF It is also degraded by nucleophilic substances like ammonia e g ammonium salts in basic conditions or acetates Polyethylene PE Agilent uses UHMW ultra high molecular weight PE PTFE blends for yellow piston and wash seals which are used in 1290 Infinity pumps and for normal phase applications in 1260 Infinity pumps Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12 5 It is compatible to many organic solvents used in chromatographic systems like methanol acetonitrile and isopropanol It has limited stability with aliphatic aromatic and halogenated hydrocarbons THF phenol and derivatives concentrated acids and bases For normal phase applications the maximum pressure should be limited to 200 bar 1290 Infinity Flexible Cube User M anual 79 5
105. s These configurations optimize the system flow path ensuring minimum delay volume For other possible configurations please refer to the Agilent 1290 Infinity System Manual 1290 Infinity Flexible Cube User M anual Installing the M odule Optimizing the Stack Configuration One Stack Configuration Instan Pilot Solvent cabinet Detector T o cooo CCOO Column compartment Autosampler Flexible Cube Figure 13 Supported One Stack Configuration Front View 1290 Infinity Flexible Cube User M anual 3 43 3 Installing the Module Optimizing the Stack Configuration LAN to control software CAN to Instant Pilot CAN Bus cable AC Power Figure 14 Supported One Stack Configuration Rear View 44 1290 Infinity Flexible Cube User Manual Installing the Module 3 Optimizing the Stack Configuration Supported Two Stack Configurations Two Stack Configuration Standard Instant Pilot Detector Q 9000 0600 0000 Column compartment Solvent cabinet Pump Autosampler Flexible Cube Figure 15 Supported Two Stack Configuration Front View Standard 1290 Infinity Flexible Cube User M anual 45 3 Installing the Module Optimizing the Stack Configuration LAN to control software CAN to Instant Pilot CAN Bus cable AC Power Figure 16 46 Supported Two Stack Configuration Rear View Standard 1290 Infinity
106. s with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUTDOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined as standard TTL levels 0 V is logic true 5 0 V is false e fan out is 10 input load is 2 2 kOhm against 5 0 V and output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal 204 1290 Infinity Flexible Cube User M anual Hardware Information 13 Table 12 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activiti
107. sample it might be necessary to use a second strong dissolving solvent Typically a flushing time of the needle seat of about 15 s at 4 mL min for each solvent is sufficient or at least a good starting condition 1290 Infinity Flexible Cube User M anual 95 6 Optimizing Lab Performance with Flexible Cube How to Achieve Higher Injection Volumes One way to achieve larger injections is to use a trapping column selected by a switching valve to capture and concentrate the injection before switching it i e injecting it onto an analytical column see Figure 28 on page 96 The valve can be conveniently located in the Thermostatted Column Compartment or in the Flexible Cube PumpA Autosampler Analytical column Position 1 al Detector Enrichment Pump B column PumpA Autosampler Position 2 Enrichment Pump B column Figure 28 Sample enrichment 96 1290 Infinity Flexible Cube User M anual Optimizing Lab Performance with Flexible Cube 6 How to Achieve High Throughput The column equilibration step can be a significant part of the cycle time Typically the column needs flushing with three to five times the column volume to stabilize it ready for the next injection and this can be 50 or more of the separation time in some applications It is an essential process but can be taken out of the cycle time by using automated alternating column regeneration For this a two position ten port 1200 bar valve head is required in
108. spection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 40 1290 Infinity Flexible Cube User M anual Installing the Module 3 Delivery Checklist Ensure all parts and materials have been delivered with your module The delivery checklist is shown below For parts identification please check the illustrated parts breakdown in Parts and Materials for Maintenance on page 161 Please report any missing or damaged parts to your local Agilent Technologies sales and service office Table5 Flexible Cube Checklist Description Quantity Flexible Cube 1 Power Cable 1 User M anual 1 Standard Accessory Kit see HPLC System Tool Kit on 1 page 164 Flexible Cube Accessory Kit see Flexible Cube Accessory 1 Kit on page 165 Second 1290 Infinity Flexible Cube Generic Valve Drive optional No 1290 Infinity Flexible Cube Generic Valve Drives optional For the valve drive the respective valve heads need to be ordered in addition 1290 Infinity Flexible Cube User M anual 41 3 Installing the Module Optimizing the Stack Configuration 42 If your module is part of a complete Agilent 1290 Infinity Liquid Chromatograph you can ensure optimum performance by installing the following configuration
109. strument Configuration Instrument Configuration Figure 20 Instrument configuration 62 1290 Infinity Flexible Cube User M anual UsingtheModule 5 Instrument Configuration e Configurator Instrument 1 Figure 21 Flexible Cube configuration 1 Configure your instrument by choosing Flexible Cube from the list of available modules 2 Choose your valve drive s valve head s from the list of configurable Valve Types NOTE If you choose the wrong valve configuration your system will show up in offline modus 1290 Infinity Flexible Cube User M anual 63 5 Using the Module Setting up the Flexible Cube with Agilent ChemStation 64 The setup of the Flexible Cube is shown with the Agilent ChemStation C 01 04 Depending on the controller e g Agilent Instant Pilot EZChrom Elite the screens look different This section describes the Flexible Cube settings only For information on the Agilent ChemStation or other 1290 Infinity modules refer to the corresponding documentation or the 1290 Infinity System M anual After successful load of the ChemStation you should see the Flexible Cube module as an active item If the Autosampler is linked to the Flexible Cube you will see this additional icon in the Autosampler GUI as Flexible Cube 0o g Ide M _ 0 00 Al A mL min B1 Wa Ga Position 2 Position 2 Port 1 gt 2 Port 1 gt 2 Left valve Position 2 Port 1 gt 2 Right valve Position 2 Po
110. t is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 18 Boot Resident Settings without on board LAN Mode Select sw1 sw2 sw3 sw4 SW5 SW6 SW7 sws TEST BOOT 1 1 0 0 1 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been completed 210 1290 Infinity Flexible Cube User M anual 1290 Infinity Flexible Cube User M anual e e e e e e Table 19 G Oyced ColdBpangsettings without on board LAN Mode select Ssn SW2 SW3 SW4 SW5 SW6 SW7 SW8 TEST BOOT 10 1 0 0 1 0 0 1 ad d Apg Agilent Technologies 211 13 Hardware Information Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers i
111. t to be connected to Agilent M odule to General Purpose module Green 1 BCD 5 20 Violet 2 BCD 7 80 a Blue 3 BCD 6 40 m Yellow 4 BCD 4 10 SS Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14 notconnected 15 5V Low 1290 Infinity Flexible Cube User M anual 187 12 Identifying Cables Agilent M odule to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent SignalName BCD Digit module Z 1 1 BCD 5 20 2 2 BCD7 80 3 3 BCD 6 40 4 4 BCD 4 10 5 5 BCDO 1 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 188 1290 Infinity Flexible Cube User M anual Identifying Cables 12 CAN LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN Cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection 1290 Infinity Flexible Cube User M anual 189 12 Identifying Cables External Contact Cable 190 10 6 Lee o o 7 11 One end of this cable pro
112. ten the fittings correctly Internal Needle Wash Due to adsorption of sample compounds to the surface of the needle sample discrimination and or carry over effects can occur This effect might be eliminated by having the entire gradient profile flow through the sampling loop of the Autosampler 1290 Infinity Flexible Cube User M anual Optimizing Lab Performance with Flexible Cube 6 Automated delay volume reduction ADVR and overlapped injection will reduce the delay volume but will also reduce the internal flushing of the Autosampler needle and should not be used with analytes where sample discrimination might be a problem External Needle Wash Adsorption of sample compounds to the outer surface of the needle might lead to contamination of sample vial septa and needle seat The outside of the needle can be washed using a wash vial in a specific location or the needle can be washed using the flush port If a wash vial in a tray location specified by the user is chosen then this vial should have no septum and should contain a solvent suitable for washing the sample from the needle The septum is not used to avoid wiping contamination off the needle on the downstream only to re apply it on the upstroke The needle can be dipped into the vial multiple times This will be effective in removing a small degree of carryover but for more effective washing of the outside of the needle use the flush port The flush port is located above and behind
113. the column compartment a second analytical column identical to the first and a second pump is needed As one column is being used in the separation run the other column is being flushed with the starting composition of the mobile phase gradient and to start the next injection the newly re equilibrated column is switched into the analytical flow path The two columns then alternate in this way for the whole sequence of injections The second pump is only required to flush an isocratic mixture through the column and so can be a simpler pump than the 1290 Infinity pumps For instance a 1200 Series isocratic pump would be sufficient to perform this task The setup is illustrated in Figure 29 on page 98 1290 Infinity Flexible Cube User M anual 97 6 Optimizing Lab Performance with Flexible Cube How to Achieve High Throughput Position 1 gt Column 2 Eluent 10 pump Autosampler Waste Regeneration Detector pump Column 1 Position 2 Column 2 Eluent pump Autosampler Regeneration gt Detector pump Column 1 Figure 29 Alternating Column Regeneration 98 1290 Infinity Flexible Cube User M anual 1290 Infinity Flexible Cube User M anual 7 Troubleshooting and Diagnostics Overview of the M odule s Indicators and Test Functions 100 Status Indicators 101 Power Supply Indicator 101 Module Status Indicator 102 User Interfaces 103 Agilent Lab Advisor Software 104 This chapter gives an overview about the tro
114. the module to the Agilent Data System Agilent Technologies 59 4 LAN Configuration LAN Configuration 60 The G4227A Flexible Cube is a hosted module so it has neither an on board LAN nor an interface slot for a LAN card The connection to other modules is established via CAN The G4212A Diode Array Detector is producing the most data in the stack followed by the G4220A Binary Pump and it is therefore highly recommended to use either of these modules for the LAN connection If there are no modules with on board LAN in the stack the LAN Card G1369C can be used for the hosted module The G1369C CAN must connect to CAN port of the module that hosts the LAN card typically the detector Then the hosted module CAN must connect to free CAN port of the system All other combinations with not work 1290 Infinity Flexible Cube User M anual 1290 Infinity Flexible Cube User M anual 5 Using the Module Instrument Configuration 62 Setting up the Flexible Cube with Agilent ChemStation 64 The Flexible Cube User Interface 65 The Flexible Cube User Interface in Autosampler GUI 72 Control Settings 74 Method Parameter Settings 75 Module Configuration 76 Solvent Information 78 Algae Growth in HPLC Systems 83 How to Prevent and or Reduce the Algae Problem 83 This chapter provides information on how to set up the Flexible Cube for an analysis and explains the basic settings ot Agilent Technologies 61 5 Using the Module In
115. the needle seat and a peristaltic pump delivers the wash solvent It has a volume of 0 68 mL and the peristaltic pump delivers 6 mL min which means the flush port volume is completely refilled with fresh solvent in 7 s If the flush port is selected the user can set how long the outside of the needle is to be washed with fresh solvent This may be as low as two or three seconds in routine situations where carryover is less of a problem and 10 20 s for more complete washing It is recommended that washing the outside of the needle in the flush port should be standard procedure to avoid contaminating the needle seat For samples where the outside of the needle cannot be cleaned sufficiently with water or alcohol from the flush pump use wash vials with an appropriate solvent With an injector program several wash vials can be used for cleaning The flush port and its peristaltic pump and tubing should be regularly flushed to ensure the lowest carryover For example before using the system each day prime the flush port for three minutes with appropriate solvent 1290 Infinity Flexible Cube User Manual 89 6 Optimizing Lab Performance with Flexible Cube How to Achieve Lowest Carry Over Needle Seat Back Flush Due to wear and tear in the needle seat and the rotor seal the carry over performance of the Autosampler is being reduced over time To eliminate this effect a back flushing of the needle seat can be used to restore performance
116. the valve head 3 Unscrew the valve head 4 Put the new valve head onto the valve drive such that the lobe fits to the groove see also Installing the Valve Heads on page 53 5 Screw the valve head onto the valve drive using the union nut see also Installing the Valve Head and Connecting Capillaries on page 54 150 1290 Infinity Flexible Cube User M anual Maintenance and Repair Replacing Valve Heads 10 6 Install all required capillary connections to the valve head 1290 Infinity Flexible Cube User M anual 7 Switch on the module 151 10 Maintenance and Repair Installing the Capillaries Parts required Preparations 152 The 2pos 10port valve can be used here in the same way as a 2pos 6port valve just follow the re routing diagram below The red arrows mean that you have to take the according installation diagram of the 2pos 6port valve Figure 38 on page 153 Figure 39 on page 155 Figure 40 on page 156 but have to mount for example the capillary connected to port 6 of the 2pos 6port valve at port 2 of the 2pos 10port valve The ports 1 and 8 have to be connected with a 120 mm length capillary 0 12 mm i d or 0 17 mm i d depending on the capillary kit 5067 4652 and the ports 9 and 10 need to be plugged with Plastic fittings 0100 1259 Plastic fitting 120 mm length p n Description G4231B 2pos 6port valve G4232B 2pos 10
117. the valve to the position it was in at the start of the run Increase valve position Switches the valve to the next serial position Decrease valve position Switches the valve to the previous serial position e Use valve position Switches the valve to the specified position The available positions depend on the installed valve Position Names For each valve position you can specify a description that appears on the method report and in the instrument actuals alni xj eseman saoe ho ve OS Wd bal eet peter ee wear R woke i 3a ATT J Une nt cave setot magit woken 4 tm Seer ante e Femetobie 4 100 reat ee Fte ta emee Emy ta ame Pre Dis Fie bema Opre i Oswa E E aata Mase no fete tne Pree i fior listna Chamat i Chanel E Prg Ute Phew iletin O AD Omt Arg Wie Pine iauna Oura t Coma B BT Timetable Use the Timetable to program changes in the flexible cube parameters during the analysis by entering a time in the Time field and appropriate values in the following fields of the timetable The values in the flexible cube timetable change instantaneously at the time defined in the timetable The following parameters can be changed Left valve change position Enables you to change the valve position during a run Changes in valve position are initiated at the specified time You can select from 1290 Infinity Flexible Cube User Manual 69 5 70 Using the Module Increase valve positi
118. ting of fuse holders must be avoided 1290 Infinity Flexible Cube User M anual 217 14 Appendix 218 Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendo
119. tions the radio interference limits are still met within the premises 220 1290 Infinity Flexible Cube User M anual Sound Emission Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A At Operator Position Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 1290 Infinity Flexible Cube User M anual 221 www agilent com Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Agilent Technologies 2010 2011 2012 Printed in Germany 10 2012 C1957 30000 G4227 90000 Rev B wie Agilent Technologies Index 8 8 bit configuration switch without On Board LAN 206 A accessory kit flexible cube 165 Agilent Diagnostic software 104 Agilent Lab Advisor software 104 Agilent Lab Advisor 104 Agilent on internet 222 ambient non operating temperature 33 ambient operating temperature 33 analog signal 203 analog cable 182 apg remote 204 autosampler user interface flexible cube 72 back flush needle seat 90 BCD cable 187 bench space 32 bio inert 54 materials 27 C cable analog 182 1290 Infinity Flexible Cube User M anual Appendix 14
120. to a wrong position can damage the column or bias measurement results gt Fit the lobe to the groove to make sure the valve is switched to the correct position CAUTION Valve Damage Using a low pressure valve on the high pressure side can damage the valve gt When using multiple column compartments as part of a method development solution make sure that the high pressure valve head is connected to the autosampler and the low pressure valve head is connected to the detector CAUTION Sample degradation and contamination of the instrument Metal parts in the flow path can interact with the bio molecules in the sample leading to sample degradation and contamination gt For bio inert applications always use dedicated bio inert parts which can be identified by the bio inert symbol or other markers described in this manual gt Do not mix bio inert and non inert modules or parts in a bio inert system 1290 Infinity Flexible Cube User M anual Installing the Module 3 Installing the Valve Heads NOTE OTE The tag reader reads the valve head properties from the valve head RFID tag during initialization of the module Valve properties will not be updated if the valve head is replaced while the module is on Selection of valve port positions can fail if the instrument does not know the properties of the installed valve NOTE To have the valve correctly recognized by the Flex Cube you must have the module pow ered
121. tten agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 J une 1995 oras a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 J une 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 J une 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 J une 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in
122. ube User M anual Appendix 14 225 14 Appendix flush pump delivery test evaluation 131 flush pump inlet valve exchange 139 frequency range 33 G general error messages 107 H humidity 33 injection valve rinse 92 installation module 49 installation bench space 32 site requirements 29 installing capillaries 152 instrument layout 212 interfaces 199 internet 222 L LAN configuration 60 LAN cable 189 leaksensoropen 110 leak sensor short 109 leak 112 line frequency 33 line voltage 33 lost CAN partner 109 M maintenance 226 1290 Infinity Flexible Cube User M anual feedback 213 introduction 134 replacing firmware 159 159 materials bio inert 27 message cover violation 114 ignition without cover 112 112 initialization of valve failed 117 position cluster partner missing 119 pressure cluster partner missing 119 remote timeout 108 valve switching failed 118 valve tag violation 118 method parameter settings 75 N needle seat back flush 90 needle wash external 89 internal 88 non operating altitude 33 non operating temperature 33 0 operating Altitude 33 operating temperature 33 optimization stack configuration 42 P packaging damaged 40 parts identification cables 179 parts damaged 41 1290 Infinity Flexible Cube User M anual Appendix 14 227 14 Appendix missing 41 overview 162 system tool kit 164 physical specifications 33 power consideration 30 powerconsu
123. ubleshooting and diagnostic features and the different user interfaces ot Agilent Technologies 99 7 Troubleshooting and Diagnostics Overview of the M odule s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Tests and Calibrations Diagnostic Signals The module has several signals internal temperatures voltages and currents of lamps that can be used for diagnosing baseline problems These can be added like normal signals in the Agilent ChemStation software 100 1290 Infinity Flexible Cube User M anual Troubleshooting and Diagnostics 7 Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status Status indicat
124. ulti Column Selection With the 6 position selection valve G4234A B and the capillary kit for Column Selection you can set up your system for use with up to 6 columns as displayed in Figure 7 on page 20 Or you can use the system with 5 columns and one flow path for flow injection analysis or for flushing the system This setup allows you to switch between these columns for faster method development or method validation The multi column setup might also be used if several operators are sharing system Auto Pump sampler Figure7 Multiple Column Selection Example of Schematic Setup for 6 Column Selector 20 1290 Infinity Flexible Cube User M anual Introduction 1 Sample Enrichment and Sample Cleanup 2pos 6port valve G4231A B and 2pos 10port valve G4232A B Advantages Easy automation of sample preparation Higher reproducibility Increased productivity and sensitivity Sample cleanup is essential for samples with complex matrices such as biological fluids food extracts and waste water Before injection into a LC or LC MS system the sample matrix must be separated from the analytes of interest Otherwise contaminants can disrupt separation and detection or even damage the analytical column 1290 Infinity Flexible Cube User M anual 21 1 Introduction Valve solution Valve technique PumpA Autosampler Analytical column Position 1 ul Detector Enrichment Pump B column Pump A Autosampler
125. ument Check the instrument for defects refer to the showing the not ready condition instrument s documentation Lost CAN Partner Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected e Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system Leak Sensor Short Error ID 0082 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor 1290 Infinity Flexible Cube User M anual 109 8 Error Information current to change within defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Defective leak sensor Please contact your Agilent service representative 2 Leak sensor incorrectly routed being Please contact your Agilent service pinched
126. unters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EM F Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1290 Infinity Flexible Cube User M anual 213 13 Hardware Information Early Maintenance Feedback 214 1290 Infinity Flexible Cube User M anual Appendix 14 aintenan 14 Appendix General Safety Information 215 The Waste Electrical and Electronic Equipment W EEE Directive 2002 96 EC 219 Radio Interference 22
127. valve Flush port Figure 25 Potential Sources of Carry Over 1290 Infinity Flexible Cube User M anual 87 6 88 Optimizing Lab Performance with Flexible Cube 1 Adsorption to needle and capillary material or bad design 2 Needle seat design improper sealing worn out 3 Valve Rotor Stator bad design wear 4 Capillary fittings misadjusted at all connections contaminated with the sample 5 Column related carry over all fittings and connections of the column packing stationary phase sample interaction The Agilent 1290 Infinity Autosampler is optimized for lowest carryover by careful design of the flow path and use of materials in which sample adsorption is minimized A carryover amount of 0 002 should be achievable even when a triple quadrupole mass spectrometer is used as detector Operating settings of the Autosampler allow the user to set appropriate parameters to minimize carryover in any application involving compounds liable to stick in the system The following functions of the Autosampler can be used to minimize carryover Internal needle wash External needle wash Needle seat back flush Injection valve cleaning The flow path including the inside of the needle is continuously flushed in normal operation providing lowest carryover for most applications Not correctly applied fittings are the sole largest contributor to system carry over Make sure to use correct fittings and to tigh
128. ve head Rotor seal Stator heads Method development Valve Head 8 Rotor Seal PEEK Stator head valves kit G4230A 2 Position 9 Port 600 5067 4111 5068 0001 bar 5067 4107 Method development 8 pos 9 port valve Rotor seal Vespel Stator head valves kit G4230B 2 head high pressure 5068 0002 5068 0001 1200 bar 5067 4121 2pos 6port valve 2 pos 6portultrahigh Rotor seal Vespel Stator head G4231B pressure 1200 bar 5068 0008 5068 0006 valve head 5067 4117 2pos 6port valve Valve Head 2 Postion Rotor Seal PEEK Stator head G4231A 6 Port 600 bar 0101 1409 0101 1417 5067 4137 2pos 10port valve 2 pos 10 port ultra Rotor seal Vespel Stator head G4232B high pressure 1200 5068 0012 5068 0011 bar valve head 5067 4118 2pos 10port valve Micro Valve Head 2 Rotor Seal PEEK Stator Head G4232A Position 10Port 600 0101 1415 0101 1421 6 column selector valve G4234A 6 column selector G4234B bar 5067 4144 Valve head 6 column selector 600 bar 5067 4146 Valve head 6 column selector 1200 bar 5067 4142 Rotor seal PEEK 5068 0076 Rotor seal Vespel 5068 0067 Stator Head 5068 0077 Stator Head 5068 0077 1290 Infinity Flexible Cube User M anual Parts and Materials for Maintenance 11 Valve kits include the valve head optional capillary kits manual access material and installation and familiarization service For more details refer to the Parts an
129. ve head for correct installation e Try to identify the source of trouble by installing a different valve head if possible Contact your Agilent Service representative 1290 Infinity Flexible Cube User M anual 117 8 Error Information Valve Switching Failed Error ID 24001 The valve drive was not able to operate the valve head correctly Either due to mechanical reasons or the movement couldn t be detected correctly Probable cause 1 Mechanical problems Friction too high or blockages on the valve drive s motor or on the valve head 2 Defect Sensor on the Valve Drive M otor Valve Tag Violation Error ID 24006 The valve drive identified a different during the last initialization Probable cause 1 A valve head has been exchanged hot plugged while the valve drive was still powered on Suggested actions Chec k valve head for correct installation Try to identify the source of trouble by insta ling a different valve head if possible Contact your Agilent Service representative Chec k valve head for correct installation Try to identify the source of trouble by insta ling a different valve head if possible Contact your Agilent Service representative valve head than it had identified Suggested actions Change the valve head It is important to have the valve switched off for at least 10 s after or before a new valve head has been installed Soft power down power supply
130. vides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent M odule Interface Board to general purposes p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 1290 Infinity Flexible Cube User M anual e J e e i E 090 Agilen M odubedoPC a o Ove e e e J e Pin G1530 60600 RS232 61601 5181 1561 1290 Infinity Flexible Cube User M anual Description RS 232 cable 2 m RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null M odem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 RS 232 cable 8 m Apg Agilent Technologies 191 12 Identifying Cables Agilent 1200 M odule to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control M odule 192 1290 Infinity Flexible Cube User M anual Hardware Information 13
131. ximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EM C regulations 1290 Infinity Flexible Cube User M anual 197 13 Hardware Information Electrical Connections Rear view of the module CAN Bus CAN DC Out Figure 55 Rear view of the Flexible Cube 198 1290 Infinity Flexible Cube User M anual Interfaces Hardware Information 13 The Agilent 1200 Infinity Series modules provide the following interfaces Table 10 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog optional on board APG Special Remote Pumps G1310B Iso Pump 2 Yes No Yes 1 G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump G4220A B Bin Pump 2 No Yes Yes No G4204A Quat Pump G1361A Prep Pump 2 Yes No Yes No Samplers G1329B ALS 2 Yes No Yes No G2260A Prep ALS G1364B FC PS 2 Yes No Yes No G1364C FC AS G1364D FC uS G1367E HiP ALS G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio ine
132. your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1290 Infinity Flexible Cube User Manual 31 2 Site Requirements and Specifications Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Bench Space The module dimensions and weight see Table 2 on page 33 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your modu

1290 Infinity Flexible Cube - User Manual

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