Hematocrit Centrifuge Model 410E User Manual
Contents
1. 0 cccccccesscesseeeesseeesssoessseeessaeesseeeesseeessesesseeussaeesseesseeeseness GF Graham Field and Grafco are registered trademarks of GF Health Products Inc Packaging warranties products and specifications are subject to change without notice GF Health Products Inc is not responsible for typographical errors 410E Centrifuge 2 INTRODUCTION Your Grafco Hematocrit Centrifuge has been designed to be reliable safe and easy to use This manual contains important operation and safety information Carefully read and understand the contents of this manual before install ing or using the Grafco Hematocrit Centrifuge Always install this centrifuge in accordance with local electrical parameters Product Overview timer LED lid digital display brake OR unlocking button ON OFF timer set button switch ten minute increments timer set button one minute increments spin start button 410E Hematocrit Centrifuge The Grafco Hematocrit Centrifuge shown above features a digital timer up to 99 minutes fixed speed brake control and a reading scale This unit spins loads at a fixed speed of 12 000 RPM and includes a thermostat to regulate tem perature The Grafco Hematocrit Centrifuge can hold up to 24 capillary tubes of size 1 2mm or 1 5mm x 75mm 410E Centrifuge 3 Centrifugation of anticoagulated whole blood results in the packing of red blood cells The hematocrit reading is the2. 1 Unplug the unit and disconnect the power cord from the unit 2 Clean the outer case rotor lid and rotor tube holder grooves with a moist clean cloth 3 Ensure the equipment is thoroughly dry Attach the product s electrical cord to the cord port located on the back of the centrifuge 4 Plug the centrifuge s power cord into a properly ground ed 110V 60Hz electrical wall outlet ACaution Do not immerse the centrifuge in or saturate it with water ACaution DO NOT use benzine or paint thinner for cleaning Transportation ACaution When transporting the unit place in secure packaging preferably the original packaging material which will prevent the unit from tipping over ACaution Do not drop the centrifuge turn it upside down or subject it to vibration ACaution Transport within temperature limitations listed in following SPECIFICATIONS section 410E Centrifuge 13 Storage ACaution Do not place this equipment in a draft sun light or near a piece of equipment which emits heat or electromagnetic conduction emission ACaution Disconnect power supply when the centrifuge is not in use ACaution DO NOT place other equipment or objects on top of the centrifuge ACaution Store within temperature limitations listed in following SPECIFICATIONS section SPECIFICATIONS Model Grafco 410E Hematocrit Centrifuge CE marking Conforms to LVD 73 23 EEC safety standards Standard Complies with EC Elec
3. air flow on a sturdy flat level countertop with sufficient clearance to open the lid completely 2 Attach the product s electrical cord to the cord port located on the back of the centrifuge 3 Plug the centrifuge s power cord into a properly ground ed 110V 60Hz electrical wall outlet NWARNING Locate the unit so that the centrifuge will not be accidentally pulled or dislodged from the coun tertop and so the cord will not be stepped on tripped over or otherwise subjected to damage or stress SPECIMEN COLLECTION Materials Needed Capillary tubes If non anticoagulated blood is used for the procedure we recommend the use of heparinized capillary tubes If blood is already anticoagulated we recommend the use of plain capillary tubes Clay sealant Centrifuge with rotor which will accept capillary tubes and attain at least 11 500RPM Chart for determining hematocrit values Capillary Tube Specifications Capillary Tubes Heparinized I D 1 1 1 2mm wall 0 2mm 0 02mm Plain length 75mm Precalibrated I D 0 5 0 6mm O D 1 4 1 7mm Heparinized length 75mm Calibration line 60mm from one end Tube Preparation 1 Using 2 capillary tubes insert an end of each into the blood tilt slightly and allow the tubes to fill by capillary attraction to 2 3 3 4 full 410E Centrifuge 7 2 Create a sealing plug Insert the dry ends of the tubes into the sealing clay and withdraw them with a twisting
4. percentage of the volume of packed red cells in rela tion to the volume of whole blood The hematocrit tube is filled with anticoagulated whole blood one end sealed by heat or insertion into a sealing clay and then centrifuged Interpretation of the resulting Volume of Packed Red Cells VPRC may be used to detect the presence of anemia or polycythemia Glossary Hematocrit Centrifuge An instrument which uses centrifu gation to measure the ratio of the volume of red blood cells to the total volume of blood G Force The measurement for samples undergoing the stress of acceleration in a centrifuge RCF Relative Centrifugal Force The centrifugal force that the samples undergo which relies on the speed of ro tation N in RPM and the rotating radius R The rotating radius is measured from the center axis of the rotor to the extreme ends of the centrifuge tubes RCF Formula RCF 11 18 x R x N 1000 Intended use The Grafco Hematocrit Centrifuge is intended to provide laboratory tests for hematocrit values HCT in the fields of biochemistry microbic immunity genetics and blood separation as well as general clinic tests Replacement parts Replacement parts available for the Grafco 410E Hemato crit Centrifuge from your Graham Field authorized dis tributor Graham Field Item 410E 1 Replacement Carbon Brushes package of two 410E Centrifuge 4 IMPORTANT SAFETY GUIDELINES PLEASE READ BEFORE USE The safety s
5. telephone 800 347 5678 770 447 1609 fax 800 726 0601 678 291 3232 www grahamfield com July 2008 GF Health Products Inc
6. Gra co A Graham Field Brand Hematocrit Centrifuge Model 410E User Manual Read this manual before operating the 410E Hematocrit Centrifuge Save this manual for future reference 410E INS LAB RevAO8 CONTENTS INTRODUCTION e 3 PRODUCT OVERVIEW 3 GLOSSARY 4 INTENDED USE 4 REPLACEMENT PARTS esseessseseeeeeeeetna ternera tnn tns tna ta iata 4 IMPORTANT SAFETY GUIDELINES PLEASE READ BEFORE USE 5 CAPILLARY TUBE SPECIFICATIONS TUBE PREPARATION esses nnne nnn tnnt tnsa nna U Ndatan kasak Tiue N nan 7 e d 6qe 8 BALANCED LOADS we 8 LOADING THE ROTOR ss 8 9 9 SETTING THE DIGITAL TIMER OPERATING THE CENTRIFUGE eene INTERPRETING RESULTS USING THE READING SCALE 10 EXPECTED VALUE 11 LIMITATIONS AND FACTORS AFFECTING RESULTS esee 11 DISASSEMBEY ieseisstcsscszssssisseetsarscsencecescessreuastescisarseantannenties TROUBLESHOOTING eoe coatensrecrsacecdeeseocecsdecdeensecossdgccrensenerscuczoareseececeeioese hi mjzilec CLEANING TRANSPORTATION yjin ic SPECIFICATIONS gu nn nn A nn LIMITED WARRANTY
7. ge or injury caused by im proper installation or use of this product 410E Centrifuge 5 SETUP Unpacking l Check for obvious damage to the carton or its contents If damage is evident please notify the carrier and GF Health Products Inc Open the product box carefully This may include cut ting external packaging strips which surround the prod uct shipping box Also for added safety when shipping the centrifuge may sometimes be packaged inside the outer shipping box meaning two boxes must be opened Carefully remove the Hematocrit Centrifuge from the packaging including removing the plastic bag in which the centrifuge is placed for shipping purposes Do not drop the centrifuge Ensure that all of the equipment listed in Contents following is present Keep the packing materials for transportation and or storage Contents Your Grafco Hematocrit Centrifuge contains Equipment Quantity Description supplied ESTE with 4106 1 Rotor with lid Hr Detachable power cord zo eese Installation 4 Caution The operation of AC motor is affected by the 410E Centrifuge surrounding power consumption which will result in a fluctuation of speed To assure an accurate RPM and its corresponding RCF do not place this equipment in draft sunlight or near a piece of equipment that emits heat and or electromagnetic conduction emission 1 Place the centrifuge in a large open space with unen cumbered
8. he ON Off switch to ON position Setting the Digital Timer The Grafco Hematocrit Centrifuge is equipped with an LED digital display timer which allows the user to spin the load from 1 to 99 minutes Press the triangle directly below the left side of the timer to change the spin time in increments of ten 10 minutes Press the triangle directly below the right side of the timer to change the spin time in single minutes For example if the desired spin time is twenty five minutes press the left arrow twice to display a 2 on the timer and press the right arrow five times to display a five on the timer Operating the Centrifuge Z WARNING As a safety feature the centrifuge has a power shut off device to turn the power off automati cally when the lid is opened during spinning This is a safety feature of the centrifuge DO NOT open the lid when the rotor is spinning After the desired spin time is displayed on the timer press the START button to initiate rotor spinning The timer will display the spin time remaining during operation When the spin cycle is complete the unit will slowly stop spinning To accelerate the deceleration press the BRAKE SWITCH 410E Centrifuge 9 6 Once the rotor has completely stopped spinning turn the ON OFF switch to OFF position Press the unlocking button located on the front of the centrifuge to unlock the lid Open the centrifuge lid Carefully open the rot
9. lts 1 Inadequate centrifugation either time or RPM will re sult in erroneously high reading 2 Ensure that the capillary tube is sealed properly to pre vent red cell loss during centrifugation The rubber gas ket encircling the interior of the rotor must be smooth and pliable This will aid in clay sealing plug retention and reduce breakage during centrifugation 3 Take the reading from the top of the clay sealing plug to the top of the column of VPRC 4 Hemolized blood due to over anticoagulation or im proper finger stick can produce erroneous results 5 Hematocrit determinations are not accurate immediate ly following blood transfusions or blood loss DISASSEMBLY Z WARNING Disassembly or dismantling of this centri fuge is limited to authorized service personnel only Dismantling or disassembly by non qualified person nel will result in the voiding of the unit s warranty Please contact your Graham Field authorized distribu tor or customer service representative to identify a qualified technician 410E Centrifuge 11 TROUBLESHOOTING Z WARNING Unplug the centrifuge before performing troubleshooting corrective actions Z WARNING Do not disassemble or dismantle the cen trifuge Can tturn 1 Blown fuse 1 Contact Graham Field authorized centrifuge 9 Disconnected electrical distributor to replace fuse 10A ON cord 2 Connect electrical cord 3 Worn carbon brushes 3 Contact Graham Field authori
10. motion OPERATION Balanced Loads 4 Caution Your centrifuge must contain a balanced load in order to work properly Spinning unbalanced loads may shorten the life of the device and produce un predictable results Observe the following rules when loading the rotor Opposing rotor grooves must be empty or loaded with equally weighted samples e If an odd number of samples is to be spun fill a tube with water to match the weight of the unpaired sample and place it across from the unpaired sample Loading the Rotor The Grafco Hematocrit Centrifuge can hold up to 24 capil lary tubes of size 1 2mm or 1 5mm x 75mm 1 Open the centrifuge lid if centrifuge lid is locked press the unlocking button on front of centrifuge to unlock it 2 Turn the rotor lid knob counter clockwise to unlock the it Open the rotor lid 3 Carefully place the test tubes in the rotor grooves en suring the capillary tubes touch the rotor s outer rubber gasket 4 Make sure the load is balanced by placing similar tubes across from one another This will keep the rotor in bal ance see previous section Balanced Loads 5 Close the rotor lid gently Turn the knob clockwise to lock it 410E Centrifuge 8 Z WARNING Closing the rotor lid improperly may result in broken capillary tubes which could cause injury 6 Close the centrifuge lid completely Press the locking button on the side of the centrifuge to lock the lid 7 Turn t
11. or lid and remove the specimens Z WARNING DO NOT open the centrifuge lid until the rotor has completely stopped spinning Opening the lid before the rotor has stopped spinning could result in personal injury INTERPRETING RESULTS USING THE READING SCALE l Remove the capillary tubes from the rotor Take care not to let the blood drain from the tubes Place the capillary tubes on the grooves of the tube holder carefully Line up the bottom red blood cells por tion of the tube with the straight line at the bottom of the reading scale so that the start of the red blood cells portion lines up with this horizontal fixed line Move the tube holder from left to right on the reader until the top of the Leucocyte line matches up with the fixed diagonal line on the reader platform Move the adjustable bar to meet the demarcation place where the Leucocyte and red blood cells separate The diagonal line on the adjustable bar should be directly on top of the demarcation The percentage of HCT can then be read directly from the scale by reading the number the adjustable diagonal bar crosses at the right side of the reader 410E Centrifuge 10 Expected Value Normal hematocrit values will vary somewhat depend ing upon such factors as age sex size of red cells and altitude Generally accepted normal values with range of variation are e Women 36 46 e Men 42 5296 Limitations and Factors Affecting Resu
12. tatements presented in this chapter refer to the basic safety information that the operator of the centrifuge shall pay attention to and abide by There are additional safety statements in other chapters or sections which may be the same as or similar to the following or specific to the operations Z WARNING Indicates a potential hazard situation or unsafe practice that if not avoided could result in death or serious personal injury ACaution Indicates a potential hazard or unsafe prac tice that if not avoided could result in minor personal injury or product property damage Note Provides application recommendations or other useful information to ensure that you get the most from your product Z WARNING Important Read and understand these instructions before installing or using the Grafco 410E Hematocrit Centrifuge If you do not understand any part of these instructions contact your medical pro fessional or Graham Field authorized distributor for direction in the use of this product Z WARNING For the safety of both the operator and ser vice personnel take care when handling substances that are known to be toxic radioactive or contaminat ed with pathogenic microorganisms when using this centrifuge NWARNING If components are damaged or missing contact your Graham Field authorized distributor im mediately DO NOT use substitute parts Z WARNING GF Health Products Inc assumes no re sponsibility for any dama
13. tromagnetic Compatibility requirement of EN60601 1 2 EMI EMI filter is designed in conformity with 89 336 EEC EMC Directive is ee ore ee ae ES in EE with 89 336 EEC EMC Directive Ww EEE poor Wem Baie Wad SCS S C cl 410E Centrifuge 14 LIMITED WARRANTY Your Grafco Hematocrit Centrifuge product is guaranteed to be free from defects in materials and workmanship for one 1 year under normal use from the date of purchase This WARRANTY does not apply to any product damaged by misuse abuse neglect improper line voltage dropping fire water submersion or saturation The warranty is void ed if non authorized representatives service or dismantle the centrifuge The warranty is voided if damage occurs as a result of the lid being opened when the rotor is spinning The liability of GF Health Products Inc is limited to re pair or replacement and under no circumstances shall GF Health Products Inc be liable for any collateral con sequential damages or losses This guarantee specifically excludes the expendables and consumables Direct warranty claims to GF Health Products Inc must be directed to the distributors or agents authorized by GF Health Products Inc responsible for the sale of this equip ment Manufactured for GF Health Products Inc 410E Centrifuge 15 USA Corporate Headquarters GF Health Products Inc 2935 Northeast Parkway Atlanta Georgia 30360
14. zed distributor to replace carbon brushes Erratic Worn carbon brushes Contact Graham Field authorized speed distributor to replace carbon brushes Unbalanced rotor Balance rotor load Imbalance 1 Tubes are improperly 1 Balance load positioned unbalanced load screw rotor knob to lock lid 2 Rotor lid is not locked Broken test 1 Tubes are improperly 1 Balance load tubes positioned unbalanced load place tubes against rubber gasket 2 Tubes are not placed all on rotor the way down 3 Ensure rotor lid is placed flat 3 Rotor lid is tightened against rotor incorrectly MAINTENANCE Z WARNING Centrifuge disassembly or dismantling is limited to authorized service personnel only Z WARNING Always use a properly grounded electrical outlet of correct voltage and current handling capacity ACaution Do not lubricate the drive motor or linkage assembly ACaution Do not immerse the centrifuge in or saturate it with water ACaution Do not drop the centrifuge turn it upside down or subject it to vibration 410E Centrifuge 12 ACaution Clean the chamber and rotor after each use ACaution In order to spin balanced loads the centrifuge must be level To this end confirm the rubber supports are in good condition before each use ACaution Contact your Graham Field authorized distribu tor to replace the carbon brushes yearly or as needed Cleaning Clean the equipment as described below after each use
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