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Agilent 218 Purification Solution System User

1. Tetrahydrofuran Toluene Trichloroethane Water Xylene v v o Z e 2 __ le 2 EJES o Q amp c o x rs o 5 o C S 2l56 6 2 3o o s Sia _ lclalSlEl olol SiS se S le 5l3lels lel fslsis 82 lalele l oO zT pad Z jc aak 4 Slo Ele 821s 5 5 815 SiS o 8 8 ola y oF o o S S 2 Ss 0 9 cje SIClSlelalS SlElElclSlolSHic 2icicja 15 22 6 58 2 SIL fSlolLllOislhsla lel lS lS lSlzlSlBlelCleiSrslolelalc S 2L2ILIN S 2 2 o o 8 o fE HIElE lala Z SIPS fololS SlelelS 21219 al x S SBE Jele g 5 S S 5 le 8 8 8 9 555 gt SL L E ElL S S o a L L Olo lo ltd lo lE S S lt lt lt la la lo lo JSA G G G G G OIG IT T 2 S 2 A 2 A E EA EE Figure 32 Solvent miscibility of some common solvents 146 Purification Solution System User Guide Appendix 6 Solvent Compressibility The values in Table 12 on page 147 should be used for the Agilent 218 Purification Solution compressibility factors when you are setting up the pumping system parameters For details on how to set up the pump system parameters refer to Agilent 218 Solvent Delivery Module User Manual p n G9300 90000 Table 12 Compressibility factors Solvent x L Water 46 3231 Acetone 128 9 956 Acetonitrile 97 4 1212 Benzene 96 7 1046 Carbon tetrachloride 106 7 998 Chloroform 97 4 1227 Cyclohexane 1
2. Purification Solution System User Guide 121 3 Installation Setup the System with OpenLAB CDS ChemStation Edition Control Panel 8 Set PS 325 parameters W P5325 S Fraton colector NOTE The Run time must be defined to the detector parameters 9 Click OK to confirm method 122 Purification Solution System User Guide Installation 3 Setup the System with OpenLAB CDS ChemStation Edition Control Panel 10 Set Fraction collector 11 Click OK to confirm method Setup is completed Purification Solution System User Guide 123 3 Installation Setup the System with OpenLAB CDS ChemStation Edition Control Panel 124 Purification Solution System User Guide Purification Solution System User Guide ee 4 Using Troubleshooting Maintenance and Parts Using Troubleshooting Maintenance and Parts 126 This chapter provides information on how to access further details on the system components gy Agilent Technologies 125 4 Using Troubleshooting Maintenance and Parts Using Troubleshooting Maintenance and Parts 126 For details on using troubleshooting maintenance and necessary parts for the individual modules please refer to the according manuals Agilent 218 Solvent Delivery Module User Manual p n G9300 90000 Agilent 325 UV VIS Dual Wavelength Detector User Manual p n G9309 90000 Agilent 410 Autosampler User Manual p
3. Installation 3 Fuses and voltage selector 2 Main input 3 Main switch 4 P1 I 0 connector 5 P2 I 0 connector 6 RS 422 communication interface connector 7 CE mark 8 Fan only if optional Peltier tray cooling option is installed Installing the Sample Tray 1 Place the sample tray in the autosampler and rotate it until the tray drops into the slot only one position is possible 2 Turn the tray fixation knob clock wise to fixate the tray see Overview of the Module on page 82 for an overview Connecting the Waste Tubings The following Waste Tubings must be connected to the autosampler e General waste tubing e Syring waste tubing e Water and leakage drain Connecting the General Waste Tubing 1 Connect the drain tubing to the right hand drain hose connector of the autosampler and put it in a bottle which is placed on the floor All the liquid dispensed while the autosampler is in the wash position is removed through this drain Purification Solution System User Guide 85 3 Installation CAUTION Connecting the Syringe Waste Tubing 1 Place the syringe waste tubing into a small bottle next to the autosampler If no injection volumes are programmed that are larger than the buffer tubing can contain the syringe waste will only be wash solvent Connecting the Water and Leakage Drain All solvents that result from a leak in the system and condensed water are drained through the left hand hose conne
4. Plumbing Connections IETT Damage to the pump heads gt Each individual pump head has a different set of tubing that comes with the pump head for connection to the solvent reservoir and to the rest of the system They also have individual nuts bushings and ferrules that are used to connect the pump to the rest of the system Use those parts to make the connections gt If you want to run the pump without liquid flow remove the liquid head or use demo mode Purification Solution System User Guide 53 3 Installation Pump Head Low Pressure Tubing and Inlet Filter Assembly The four types of inlet filter assemblies are shown below 10 mL min Heads a ee i Install filter on tubing To pump inlet 25 mL min Head Tefzel a translucent Delrin a white Bie _ To pump inlet Install filter on tubing T Flangeless fitting amp ferrule 100 mL min Heads Delrin aS s P To pump inlet Install filter on tubing Flangeless fitting amp ferrule 200 mL min Head Delrin fitting xN wa 1 1 Flangeless fitting amp ferrule To pump inlet Figure 7 Inlet filter assembly installation Installation of inlet tubing for 10 to 100 mL min heads 1 Remove the solvent inlet assembly from the accessories package and immerse the inlet filter into clean HPLC grade water 2 Assemble the inlet filter onto the end of the inlet tubing 54 Purification Solution System User Guide Installation 3 3 Connect the inle
5. Corfiguraton Q aer ps amp Ames IPS2 S011 9 HH eao P5225 EL amp hs Frecten collector HA0FC AYTIOIMOTS H once l J ho Foon Cotector Generic Modules emt crise ceriey wx Aak for configuration change at Oen Riton satus 2 Click OK You re back in Agilent OpenLab Control Panel Purification Solution System User Guide 3 117 3 Installation up the System witl ion Edition Control Panel Setup Method 1 Select Launch to start the OpenLab CDS Chemstation Edition is i i ssi 7x hx X Edit Delete Refresh Lock Create Configure Edit Notifications Shortcuts Instrument Instruments and Locations Properties Actions Navigation Not Connected mam Instruments X PrepLe gt Launch Offline EL instruments X Administration Your startup license expires in 60 days 118 Purification Solution System User Guide Installation 3 Setup the System with OpenLAB CDS ChemStation Edition Control Panel 2 Set Pumps parameters 3 Click OK to confirm method Purification Solution System User Guide 119 3 Installation Setup the System with OpenLAB CDS ChemStation Edition Control Panel 4 Set Autosampler parameters 5 Click OK to confirm method 120 Purification Solution System User Guide Installation 3 Setup the System with OpenLAB CDS ChemStation Edition Control Panel 6 Set Autosampler Injector Program 7 Click OK to confirm method
6. damage and accuracy of the module gt Select an area free from drafts corrosive atmospheres and vibration gt Select a dust free low humidity environment gt Use air conditioning for control of the environment Bench Space Make sure that the bench is designed to bear the weight of all modules For details on the space needed around the individual modules refer to the according manuals e Agilent 218 Solvent Delivery Module User Manual p n G9300 90000 Agilent 325 UV VIS Dual Wavelength Detector User Manual p n G9309 90000 Agilent 410 Autosampler User Manual p n G9331 90000 e Agilent 440 Fraction Collector User Manual p n G9340 90000 20 Purification Solution System User Guide Site Requirements and Specifications 2 Specifications For details on specifications of the individual modules please refer to the according manuals e Agilent 218 Solvent Delivery Module User Manual p n G9300 90000 e Agilent 325 UV VIS Dual Wavelength Detector User Manual p n G9309 90000 e Agilent 410 Autosampler User Manual p n G9331 90000 Agilent 440 Fraction Collector User Manual p n G9340 90000 Purification Solution System User Guide 21 2 Site Requirements and Specifications Specifications 22 Purification Solution System User Guide Purification Solution System User Guide 3 Installation Delivery 26 Damaged Packaging 27 Check Delivery 28 Delivery Checklists 28 Unpacking and I
7. 2 Lift and slide out the top of the door 80 Purification Solution System User Guide Installation 3 Installing the Autosampler Location of the Autosampler Module The best place to install your Agilent 410 Autosampler is at the top of the module stack This stack would normally be solvent delivery module on the bench detector on top of the SDM and finally the AutoSampler From the right hand side you have the shortest connection to the injection valve of the Agilent 410 Autosampler to the column Purification Solution System User Guide 81 3 Installation Installing the Autosampler Overview of the Module Figure 18 Autosampler overview 82 Purification Solution System User Guide Installation 3 FP wo N gt ol 11 12 13 Vial wash drain Condensed water and leakage drain Tray fixation knob Sample tray Syringe dispenser Keyboard Buffer tubing Needle arm Injection valve Oven compartment Tubing guide Wash position Position for transport solvent and reagent vials The rear view of the autosampler is shown in Figure 19 on page 84 Purification Solution System User Guide 83 3 Installation Installing the Autosampler WARNING RISK OF ELECTRIC SHOCK DISCONNECT POWER BEFORE SERVICING AVERTISSEMENT RISQUE DE CHOC ELECTRIQUE COUPER L ALIMENTAT TION AVANT LA MAINTENANCE Figure 19 Rear View of the autosampler 84 Purification Solution System User Guide
8. For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the modules for download 154 Purification Solution System User Guide Index 3 3 way pump head prime valve installation 51 A AC power 71 Agilent on internet 154 analog output 130 assembling fraction collector 93 autosampler connecting 89 lifting 32 location 81 service 13 B battery safety information 149 C cables other 70 power 70 checklist delivery 28 pump 28 column oven 13 communication cabling connecting 63 compliance CE 151 components 92 condensation 20 connections workstation control 129 connector external contacts 65 connectors external 65 D defect on arrival 27 delivery checklist autosampler 29 detector 28 delivery checklist 29 fraction collector 29 detector outlet back pressure restrictor 78 driver installation 109 E electronic waste 148 F features 11 flowcell types 74 flowcell install 75 G general waste tubing connecting 85 Purification Solution System User Guide Index hardware setup 104 high pressure tubing pump head 56 high pressure hazard 145 inlet filter assembly 54 inspection 30 installation internal mixer 44 mast kit 46 overview 93 pressure module 41 probe 95 pump head 39 tubing 95 installing door 79 internal mi
9. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctl
10. any time PA H Figure 5 Pump head installation Description Hex wrench 1 4 in 200 mL min head only 1 Remove the shipping plug from the liquid head aperture on the front of the pump 2 Insert the pump head into the front aperture of the pump The notch at the bottom of the pump head body must fit the matching pin on the pump below the aperture This notch ensures that the inlet and outlet ports are located in the correct position 3 Holding the pump head in place slide the clamp found in the pump head kit down over the head so the clamp flanges engage the slots on both sides of the pump head Purification Solution System User Guide 39 3 40 Installation Depending on the position of the pump cam you may have to push the pump head in to get the clamp on Make sure that the clamp flanges are in their slots on both sides and finger tighten the thumbscrew until the clamp holds the pump head securely In the case of the 200 mL min head use the supplied hex wrench to tighten hex screw on the clamp Tighten very securely Repeat this procedure for each pump head in your system There are three sizes of pump head clamps The smaller one fits all pump heads that do not have piston wash The larger one fits all heads that have piston wash The largest clamp is used only with the 200 mL min head 4 Enter the pump head size into the pump software and into the pump firmware from the key pad For details see Setting th
11. bubble prevention and to prevent evaporation of volatile samples 3 Check seal after crimping if the cap can be turned easily the seal is not airtight re adjust hand crimper When using uncapped vials the performance of the AutoSampler may not meet the specifications precision Do not re use a sample vial without replacing its cap or septum Purification Solution System User Guide 89 3 Installation Loading the Sample Tray 1 Place the vial in the tray and link them to methods in the Series For details see Agilent 410 Autosampler User Manual p n G9331 90000 Chapter Using section Programming the Run Sequence or Series 2 Ifthe autosampler ist not running manually rotate the tray to gain access to all vial positions 90 Purification Solution System User Guide Installation 3 Installing the Agilent 410 Autosampler Prep Option 1 Replace the standard injection valve with the special Agilent 410 Prep valve 2 Replace standard sample needle air needle and buffer tubing with the ones supplied in Prep Upgrade Option Includes Needle Syringe Injection Valve and Large Volume Sample Tray p n 393590791 Re connect all tubing to the injection valve Replace standard Syringe with the 2500 uL syringe Install the 24 vials LSV tray Choose Prep Mode in system ao oa A Q Purification Solution System User Guide 91 3 Installation Installing the Fraction Collector Installing the Fraction Collector Overview
12. cable can be used if available for the particular application Purification Solution System User Guide 137 5 Cables Synchronization Signals Figure 31 The 0 adapter board 138 Purification Solution System User Guide Purification Solution System User Guide 6 Appendix General Safety Information 140 Solvent Miscibility 146 Solvent Compressibility 147 The Waste Electrical and Electronic Equipment Directive 148 Batteries Information 149 Radio Interference 150 CE Compliance 151 Electromagnetic Compatibility 152 Agilent Technologies on Internet 154 This chapter provides addition information on safety legal and web ee Agilent Technologies 139 6 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual 140 Purification Solution System User Guide Appendix 6 Informat
13. hold the top of the piston steady with another wrench Purification Solution System User Guide 61 3 Installation Analytical PEEK and Narrowbore Analytical Titanium Mixers Plumbing Fittings Extra long fitting Inlet fitting and ferrule Figure 11 Compression fittings for 1 2 mL analytical PEEK and 0 6 mL narrowbore and 1 2 mL analytical titanium mixers Do not use tools to tighten 1 Slide the 0 159 cm 1 16 in OD outlet tubing through the one piece fitting and ferrule and push the end of the tubing into the port as far as possible 2 Holding the tubing in place finger tighten the fitting 3 For both inlet ports place a fitting and ferrule on the tubing and tighten in the same manner as the outlet fitting 62 Purification Solution System User Guide Installation 3 Preparative PEEK Titanium Mixers Plumbing Fittings Tools required Fitting Ferrule collar Ferrule Figure 12 Compression fittings for 10 mL preparative PEEK and titanium mixers Description Wrench 5 16 in All ports are identical 1 Slide the fitting and ferrule ferrule collar over the 0 318 mm 1 8 in OD tubing as shown and push the end of the tubing into the port as far as possible 2 Holding the tubing in place finger tighten the fitting When tightening the outlet port fitting hold the piston steady with another wrench 5 16 in When the system plumbing is complete check for any leaks If a leak is found stop the pumps and tight
14. included in the probe kit The longer piece of tubing with a nut and ferrule is used for the drain tube on the divert valve The shorter tubing with a nut and ferrule is used to connect fraction collector divert valve to the sample output from the HPLC system The third piece of tubing is 0 025 cm 0 010 in ID tubing and can be used for low delay volumes for applications with flow rates of 2 mL min or less For details on installing the tubing refer to Agilent 440 Fraction Collector User Manual p n G9340 90000 1 Turn off the fraction collector 2 Slide the Z axis slide to the top of the probe carriage 3 Manually rotate the probe arm so that it can be easily accessed Purification Solution System User Guide 95 3 Installation 4 Disassemble the probe kit by unscrewing the nut holding the metal probe from the bottom of the lower knurled mount nut and then the lower knurled mount nut from the top knurled mount nut Top knurled mount on the probe assembly I Lower knurled mount nut on the probe assembly 5 Insert the probe into the probe mounting block see Figure 20 on page 92 Push the tubing down into the probe so that at least 0 5 cm tubing protrudes from the end of the probe Secure the lower knurled mount nut to the top knurled mount nut Secure the nut holding the metal probe into the bottom of the lower knurled mount nut 9 Clip the tubing into the tube restraint w
15. installed by the user where dead volume and low holdup are critical is the line from the column exit to the flowcell inlet port This line should be as short as possible The Agilent 325 Detector can be fitted with any one of four flowcells ordered separately Each one has an inlet and outlet connection and quartz optics cell window Your chosen flowcell type is packed internally in the detector The four flowcell types are outlined in Table 8 on page 74 Table8 Compatible flowcells Flowcell type Flowcell p n pathlength Column ID Flow rate Maximum pressure Analytical 210181800 9mmxOmm 2 8mm 0 0001 69 bar 10 mL min 1000 psi Preparative 210181900 9mmximm 4 76mm 1 69 bar 500 mL min 1000 psi Scale Up 210224200 4mm x 4 76 mm 10 69 bar 0 25 mm 200 mL min 1000 psi Super Prep 210182000 4mm x 8 152 mm 30 69 bar 0 15 mm 1200 mL min 1000 psi Micro analytical 210182100 4mmxO0mm 1 4mm 0 20mL min 69 bar 1000 psi A pathlength of a mm x b mm means that the sample light path has a pathlength of a mm and the reference light path has a pathlength of b mm A reference light path of zero means there is no fluid in the cell air acts as the reference Extended range cell It is important for the extended range cells to run at least at the minimum flow rate 74 Purification Solution System User Guide Installing a Flowcell Installation 3 The Agilent 325 UV VIS Dual Wavelength Detector is not shipped with a flow
16. provides information on cables used with the instrument 6 Appendix This chapter provides addition information on safety legal and web Purification Solution System User Guide 3 Contents Contents Introduction 7 Introduction to the System 8 Introduction to the Solvent Delivery Module 9 Introduction to the Detector 11 Introduction tothe Autosampler 12 Introduction to the Fraction Collector 15 Site Requirements and Specifications 17 Site Requirements 18 Specifications 21 Installation 23 Delivery 26 Damaged Packaging 27 Check Delivery 28 Unpacking and Inspection 30 Optimizing the Stack Configuration 33 Installing the Solvent Delivery Module 37 Installing the Detector 69 Installing the Autosampler 81 Installing the Fraction Collector 92 Setup Hardware 104 Setup the System with OpenLAB CDS ChemStation Edition Control Panel 110 Using Troubleshooting Maintenance and Parts 125 Using Troubleshooting Maintenance and Parts 126 Purification Solution System User Guide Contents 5 Cables 127 Cable Overview 128 Cable Connections 129 Analog Output 130 Relay Output 131 Desktop PC Communications 134 Synchronization Signals 135 6 Appendix 139 General Safety Information 140 Solvent Miscibility 146 Solvent Compressibility 147 The Waste Electrical and Electronic Equipment Directive 148 Batteries Information 149 Radio Interference 150 CECompliance 151 Electromagnetic Compatibility 152 Agile
17. 0 3 9 34 Purification Solution System User Guide Installation 3 Optimizing the Stack Configuration Binary System Pressure module Detector Injection valve Pump master Prime purge valve aA oP 3 sf a Pump slave Low pressure solvent valve a 35 na A Mixer Figure 3 Binary System Table3 Binary system component heights on the mast measured from the bench to the bottom of the bracket Part Height in mm in Injection valve 485 19 1 Prime purge valve 385 15 2 Low pressure solvent valve 300 11 8 Low pressure solvent valve 100 3 9 Purification Solution System User Guide 35 3 Installation nizing ti Q r Pani Auto Preparative System Column Tee Detector m c7 Injection pump Q iN 4 Pump A i Pump B A Mixer Fraction collector Figure 4 Gradient Auto Preparative System JOULU The injection pump is used for automatic injection 36 Purification Solution System User Guide Installation 3 Installing the Solvent Delivery Module Electrical Setup CAUTION Damage to the module gt Use only correct fuses recommended for your voltage usage gt A label stating the operating power rating of your instrument as wired in the factory is affixed to the rear panel adjacent to the power receptacle and voltage selection assembly J1 gt The pumps are shipped with fuses instal
18. 0 VAC 10 50 60 Hz 1 Hz single phase without modification or the need to change fuses Before connecting power to the detector ensure that the power switch on the front of the instrument is OFF the rocker switch O is pressed The power switch is a rocker switch that connects from the front of the detector directly to the power receptacle on the rear panel Plug one end of the power cord into the power receptacle on the rear panel and the other end into your AC power source Purification Solution System User Guide 71 3 Installation WARNING Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region 72 All devices in the liquid chromatography system should be connected to the same power source using a properly grounded 8rd wire to earth multiple outlet power strip Do not turn on the AC power yet All required external devices and hydraulics must first be connected Avoiding Harmful interferences to Radio or Television Reception Operation is subject to the following two conditions e This device may not cause harmful interference e This device must accept any interference received including interferen
19. 14 800 Dichloroethane 111 9 1020 Diethyl ether 188 700 Dimethylformamide 80 1500 Dioxane 60 1500 Ethanol 115 1100 Ethyl acetate 100 1800 Methylene chloride 97 4 1212 Methanol 125 1200 n Heptane 144 760 n Hexane 167 2 644 o Dichlorobenzene 95 1400 Propanol 98 1200 Tetrahydrofuran 95 1500 Toluene 93 1200 2 Methylformamide 80 1500 Purification Solution System User Guide 147 6 Appendix The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 148 Purification Solution System User Guide Appendix 6 Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA IC
20. 48616 Prime purge valve bracket for stainless steel purge valve Prime purge valve bracket for PEEK purge valve While stainless steel images are shown here the procedure is the same for PEEK or Titanium prime purge valves 1 Unpack the stainless steel purge valve and purge valve bracket t ca 2 Remove the knob and nut from the purge valve The knob is held in place with a grub screw which is loosened using a 2 mm hex key Grub screw 3 Secure the valve to the valve bracket using the nut 4 Replace the knob on the valve and tighten the grub screw using the provided 2 mm hex key 5 Attach the purge valve to the mast For correct height see Isocratic System on page 34 or Binary System on page 35 50 Purification Solution System User Guide Installation Installing the 3 way Pump Head Prime Valve Tools required Description Hex key 2 mm Parts required Description Pump head prime valve Pump head prime valve bracket 3 1 Unpack the 3 way valve and bracket 3 Unscrew the nut and then remove the two washers from the 3 way valve Purification Solution System User Guide 2 Using the provided 2 mm hex key loosen the grub screw and slide off the selection knob Valve body Flat washer Ridget washer Selection knob Grub screw Nut 4 Attach the bracket so that the three holes on the 3 way valve face away from the thumbscrew on the brac
21. AO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA Purification Solution System User Guide 149 6 Appendix Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 150 Purification Solution System User Guide Appendix 6 CE Compliance Your Agilent 700 Series ICP OES instrument has been designed to comply with the requir
22. Agilent 218 Purification Solution System User Guide et Agilent Technologies Notices Agilent Technologies Inc 2012 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 69300 90300 Edition 02 2012 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein
23. Detector User Manual p n G9309 90000 Purification Solution System User Guide 77 3 78 Detector Outlet Back Pressure Restrictor The Back pressure restrictor p n 110743300 should be added to all flow cells unless a fraction collector is installed after the detector The back pressure restrictor assembly that was supplied with your detector should be threaded into the outlet line from the flowcell The back pressure restrictor applies approximately 2 76 bar 40 psi back pressure on the flowcell This prevents outgassing and bubbles from forming or being trapped in the flowcell which can cause an unstable baseline Note the arrow stamped on the restrictor body This arrow must point away from the flowcell outlet port and toward the waste receiver The threaded plastic fittings should be finger tightened only enough to prevent leaks About 122 cm 48 of 1 6 mm 1 16 tubing is supplied at both the inlet and outlet of the restrictor The inlet tubing is provided with a 1 6 mm 1 16 plastic tubing fitting for connecting to the flowcell outlet port Either the outlet tubing can be directed to the waste container or the tubing removed and the restrictor itself dropped to the bottom of the waste bottle The restrictor pressure setting is not adjustable If the restrictor fails or becomes plugged replace the existing cartridge with a new 2 76 bar 40 psi replacement Purification Solution System User Guide Installa
24. Pressure Restrictor 78 Installing the Door 79 Installing and Removing the Door 80 Installing the Autosampler 81 Location of the Autosampler Module 81 Overview of the Module 82 Installing the Sample Tray 85 Connecting the Waste Tubings 85 Starting the Autosampler 87 Rinsing the System with Wash Solvent 88 Connecting the HPLC to the Autosampler 89 Filling and Sealing the Vials 89 Loading the Sample Tray 90 Installing the Agilent 410 Autosampler Prep Option 91 Installing the Fraction Collector 92 Overview of the Module 92 Installation Overview 93 Assembling the Fraction Collector 93 Installing the Spill Tray 94 Installing the Rack Location Mat 95 Installing the Probe and Tubing 95 Assembling the Sample Racks 98 Purification Solution System User Guide Installation 3 Installing the Sample Racks 99 Rack Orientation 100 Connecting the Power to the Fraction Collector 101 Connecting the Fraction Collector to the HPLC System 103 Determining the Volume Delay 103 Determining the Correct Probe Depth 103 Setup Hardware 104 Setup the Hardware 104 Setup the Pump 105 Setup the Autosampler 107 Setup the Detector 107 Setup the Fraction Collector 108 Install Agilent OpenLAB CDS ChemStation PrepLC Drivers 109 Setup the System with OpenLAB CDS ChemStation Edition Control Panel 110 Setup the System with OpenLAB CDS ChemStation Edition Control Panel 110 Setup Method 118 This chapter gives information about the in
25. See Hydraulic Connections Flowcells on page 74 for suitable flowcells Purification Solution System User Guide Installation 3 Delivery Checklist Autosampler The autosampler is packed in a single carton The following list shows all items delivered in a standard delivery Your personal list depends on your order therefore countercheck delivery with your order e Agilent 410 Autosampler G9331A G9332A e Power cord e Agilent 410 Reservoir Rack e Standard Tray Assy G9332A e Agilent and PrepStar Mast kit e Prime purge valve bracket e Prime purge valve stainless steel Delivery Checklist Fraction Collector The following list shows all items delivered in a standard delivery Your personal list depends on your order therefore countercheck delivery with your order e Agilent 440 LC Fraction Collector e Assy USB RS232 serial adaptor e Rack e 440 LC Hard Wired Interface kit Power cord Purification Solution System User Guide 29 3 Installation Unpacking and Inspection Unpacking and Inspection 1 Check carefully to make sure you received all the items listed on the packing list 2 Carefully unpack all the containers and inspect the contents for damage as soon as possible 3 Save the packing containers they will be useful if you have to file a claim for damage or in the case of future transit Unpacking the Solvent Delivery System WARNING Danger to hands and feet The instrument is heavy
26. Solution System User Guide 133 5 Cables Desktop PC Communications 134 Communication between the detector and a desktop PC occurs by an Ethernet connection Communication by an Ethernet connection is required to control the detector remotely by OpenLAB When the Workstation provides HPLC system control the synchronization cables from P9 and J10 are not used To create an Ethernet connection insert an RJ45 cable included in the ship kit into the J1 receptacle and into the PC The Ethernet cable that comes with the detector is a cross over cable which is appropriate for connecting the detector directly to a PC Connecting the detector to a network or a hub will usually require a patch cable A Ethernet cable for use in a network p n 392612901 can be purchased from Agilent or either locally Most PCs come pre configured with an Ethernet connection which is usually built into the motherboard or with an Ethernet network card installed However if you have a PC that has no network interface you will need to install and configure a Network Interface Card PCI bus The PC must have a spare PCI slot for the installation of this device You are also responsible for setting up and maintaining any LAN configuration where a detector may be used All network issues are to be dealt with by the user Purification Solution System User Guide Cables 5 Synchronization Signals Synchronization Signals The synchronization signals at P9 an
27. ach solvent The Agilent 218 Solvent Delivery Module operates with a variety of Agilent 218 Solvent Delivery Module heads to maintain specified performance over designated flow and pressure ranges The easily replaceable pump heads are Purification Solution System User Guide 9 1 10 Introduction self contained units including a spring loaded piston and check valve cartridges Pump heads are not included with individual drive modules A complete HPLC system can be controlled either by an Agilent 218 Solvent Delivery Module or PC based software When the computer controls pumps all pumps are slaves and programming is done on the computer On the pump rear panel there is a single RS 422 male connector This connector is used for bidirectional signals to and from the controller whether the controller is an external computer or another Agilent 218 Solvent Delivery Module Internal software in the Agilent 218 Solvent Delivery Module determines whether the pump is a master controller or a slave pump The possible system configurations depending on the type of pumps and controller being used are the following e Isocratic system e Gradient system with one Agilent 218 Solvent Delivery Module as the controller When several pumps are connected together the master Agilent 218 Solvent Delivery Module can control the other pumps in the liquid delivery system A master Agilent 218 Solvent Delivery Module can control up to three other sla
28. and refill speed For more information about setting the pump ID and pump head size see Setup in Agilent 218 Solvent Delivery Module User Manual p n G9300 90000 Purification Solution System User Guide Installation 3 Installing the Detector Location of the Detector Module Place the detector conveniently near your HPLC system The modular design of the detector enables you to locate it anywhere within the limitations imposed by the length of the power cord fluid lines and signal cables In order to keep liquid dead volume as low as possible and to minimize peak broadening in the lines the distance between the column outlet and the flowcell inlet should be kept to a minimum For best performance the detector should be located on a clean sturdy vibration free bench in an area free of e Heat sources such as direct sunlight or a heater vent Drafts such as an open doorway window or air conditioner vent e Smoke or UV absorbing vapor e Corrosive or dusty atmosphere Potential liquid spills Provide approximately 4 inches of space behind the unit so that the cooling fan intake is not impeded and to allow easy access to the rear panel services see Power Connection and Rear Panel Services on page 70 Purification Solution System User Guide 69 3 Installation Power Connection and Rear Panel Services WARNING Electrical shock Risk of stroke and other personal injury gt Turn off the power to the
29. ash solvent The autosampler will give the best results if all air is removed from the syringe Purification Solution System User Guide Installation 3 Connecting the HPLC to the Autosampler To ensure reproducible injections the following connections to your HPLC system should be made 1 Connect the HPLC pump to port 1 of the injection valve 2 Connect the HPLC column to port 6 of the injection valve 3 Check for leakage and let the system equilibate for at least 5 min The instrument is flushed with Ilsopropanol Make sure that the mobile phase of your HPLC system is miscible with Isopropanol If your mobile phase is not miscible with lsopropanol then start with an intermediate solvent that is a transition to the mobile phase To avoid possible contamination of the column it is good practice to not have the column installed during this operation It is essential that the contents of the sample loop are injected as a back flush onto the column therefore do not exchange column and pump connections at the injection valve Filling and Sealing the Vials 1 Fill the standard vials as well as the conical vials by means of a narrow end pipette to allow air to escape when filling the vial Do not fill vials completely to the top In that case sample will be forced into the air needle risking cross contamination of samples and fouling of the needle pair 2 Check that the seal is airtight due to maintain a pressure on the vial for air
30. ation Solution System User Guide 55 3 Installation Pump Head High Pressure Tubing Tubing ns Fitting ER Ferrule Outlet check valve Liquid head B Figure 8 High pressure tubing connections A titanium or stainless steel heads B PEEK Heads Installation of high pressure tubing for titanium or stainless steel heads 1 Thread one of the 4 28 male nuts and ferrules onto the outlet tubing For orientation see A 2 With the nut and ferrule in place insert the tubing into the outlet check valve as far as it will go 3 Holding the tubing in place tighten the nut 1 4 turn beyond finger tight with an open end wrench to swage the ferrule to the tubing 4 Repeat this process at the other end of the outlet tubing for the next device in line mixer pressure monitor etc If the connection leaks when the system is pressurized tighten the nut slightly until the leak stops 56 Purification Solution System User Guide Installation 3 Installation of high pressure tubing for high pressure tubing for PEEK heads 1 Thread one of the polyacetal nuts and ETFE ethylene tetrafluoroethylene Tefzel 2 piece ferrules onto the outlet tubing For orientation see B 2 With the nut and ferrule in place insert the tubing into the outlet check valve as far as it will go 3 Holding the tubing in place tighten the nut turn beyond finger tight with an open end wrench to swage the ferrule to the tubing 4 Repeat th
31. ce that may cause undesired operation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try one or more of the following measures 1 Relocate the radio or antenna 2 Move the device away from the radio or television 3 Plug the device into a different electrical outlet so that the device and the radio or television are on separate electrical circuits 4 Make sure that all peripheral devices are also certified Make sure that appropriate cables are used to connect the device to peripheral equipment Purification Solution System User Guide Installation 3 6 Consult your equipment dealer Agilent Technologies or an experienced technician for assistance 7 Changes or modifications not expressly approved by Agilent Technologies could void the user s authority to operate the equipment Removing the Front Panel 1 If installed remove the door from the Agilent 325 Detector see Installing and Removing the Door on page 80 for more information 2 Remove the panel on the front right side of the detector by unscrewing the captive screw in the top left corner of the panel Figure 15 Removing the front panel Purification Solution System User Guide 73 3 Installation Hydraulic Connections Flowcells Hydraulic connections are located at the front of the Agilent 325 Detector The only line
32. cell installed You will need to install the flowcell that you purchased with the detector Each flowcell comes with a set of recommended nuts and ferrules that may be fitted to tubing 1 16 tubing is used on all flowcells However for the 4 mm x 0 15 mm super prep flowcell it is recommended to use 1 8 tubing at higher flow rates In this case you can add the Adaptor 1 8 1 16 p n 1610126800 This will require the 1 8 tubing and the 1 8 flowcell fittings Tubing connections are PEEK type except for the Super Prep flowcell which uses Tefzel tubing 0 125 mm x 0 062 mm Parts required p n 210181800 210181900 210224200 210182100 9910128300 OR OR OR 210182000 1610126900 1610126400 1610126800 a a a ee a ae a ee a Purification Solution System User Guide Description Flowcell 9 mm x 0 mm inert analytical Flowcell 9 mm x 1 mm inert prep Flowcell 4 mm x 0 25 mm inert scale up Flowcell 4 mm x 0 mm inert micro analytical Flow cell replacement fittings Flowcell 4 mm x 0 15 mm inert super prep Fitting 1 8 tube nut flat bottom Fitting 1 8 tube ferrule Pack of 10 Adaptor 1 8 1 16 75 3 Installation Installing the Detector 1 Fit the nuts and ferrules on the PEEK tubes 2 Screw the two tubes into the inlet and outlet connection of the flowcell Each flowcell has an inlet and outlet connection Thumbscrew Ferrule Nut Outlet connection PEEK tube Inlet c
33. ctor Risk of damaging the autosampler Drain and waste tubing are twisted thereby obstructing the flow path gt Be sure that the drain and waste tubing are not twisted 86 1 Connect the hose connector to a waste container on the floor Purification Solution System User Guide Installation 3 Starting the Autosampler Tools required OR Preparations p n Description Distilled water Distilled water lsopropanol Helium Helium Ultrasonic bath Allow the Agilent 410 Autosampler to reach ambient temperature for at least one hour Connect the waste tubings see Connecting the Waste Tubings on page 85 for more information Electrical shock Risk of stroke and other personal injury due to reduced safety protection or unwanted fusing gt Ensure that the code on the fuse cap matchs the information next to the fuse holders Risk of fire and damaging the module gt For 115 VAC 15 use two 5 A T fuses slow blow gt For 230 VAC 15 use two 2 5 A T fuses slow blow gt All fuses must be UL listed and CSA certified or IEC 127 type Keep the keyboard front cover closed during operation Remove the safety screw on the right hand side of the front cover Check fuses and voltage settings on the rear of the instrument Connect the power cable FP O N Turn the power switch to ON The Ready screen appears displaying the firmware revision number Purification Solution System User Gu
34. d for choices see Note above Refill 125 piston CIM 5 4 Press Enter 5 Reboot the module Purification Solution System User Guide 105 3 Installation Setup Hardware Setup Pump ID for Pump 2 if Exist 1 Turn on the Pump 2 2 Press on the Pump keyboard Setup and then Enter 3 With the left arrow key select ID of the Pump 2 available choices are 0 63 MC master controller or no ID NOTE For OpenLAB an ID between 1 63 must be used HDSZ Pump head xx depends on what pump head is installed for choices see Note above Refill 125 piston CIM 4 Press Enter 5 Reboot the module 106 Purification Solution System User Guide Installation 3 Setup the Autosampler Preparation for general and tray settings 1 Press Serial on the autosampler keyboard To set up the general system settings 1 Turn the module on 2 Press SYSTEM 3 Press GENERAL 4 Confirm or change each of the settings that appear on the display Volume of installed loop 0 5000 uL Volume of tubing needle valve 0 999 uL Syringe volume 250 1000 Syringe speed LOW NORMAL HIGH Skip missing vials YES NO Air segment YES NO Headspace pressure YES NO To set up the tray settings 1 Turn the module on 2 Press SYSTEM 3 Press Tray 4 Confirm each of the tray settings that appear on the display Tray type 84 3 9 24 Vial type STANDARD 2 5 mL 5 Reboot the module Setup th
35. d J10 are used to synchronize the operation of a group of instruments that are not interfaced to OpenLAB The synchronization signals come in four pairs and define how the detector will operate in a HPLC system These signals are important for controlling timing and synchronization of the detector with the other devices in the system Synchronization signals are closely tied to the detector states and transitions P9 and J10 pin designations are shown in Figure 29 on page 135 J10 P9 6 Start Out 15 5 Volts 8 Ground 7 Not used 14 Lamp Off 7 Lamp Off 8 Ground 13 Auto zero 6 Auto zero 9 5V 12 Start In 5 Fault In 11 Enable Out 4 Fault In 10 Enable Out 3 Ready Out 9 5 Volts 2 Ready Out 1 Start In Figure 29 Pin designations for J10 and P9 P9 provides connections when the Agilent 325 UV VIS Dual Wavelength Detector acts as a slave and receives control from another module J10 provides connections when the detector acts as a master and sends control to another device An input is activated or said to be present when its two signal wires are connected together This can be done with a relay contact closure If the inputs are driven from another instrument with optical isolators or other polarized devices then attention must be paid to the polarity of the signal wire Purification Solution System User Guide 135 5 Cables connections The posit
36. detector before making power and signal cable connections The detector can communicate with other modules and devices in the entire HPLC system All power and signal connections are made on the rear panel of the detector see Figure 14 on page 70 The connectors on the rear panel support communication configurations to a wide range of Agilent modules and non Agilent devices qm ce C amp ely 5 SA TE Main power receptacle J1 Comm J4 Relay out J14 Analog out P9 Sync signal J10 Sync signal Figure 14 Agilent 325 Detector rear panel services The following table summarizes the function of each connection 70 Purification Solution System User Guide Installation 3 Table 7 Rear panel services functions Connection Function Main power receptacle 3 pin receptacle with 2 fuses J14 Analog out 9 pin female D shell connector used for two channels A and B of analog output J4 Relay out 15 pin female D shell connector used for time programmed contact closures J1 Comm RJ 45 type connector used to interface the Agilent 325 to a desktop PC P9 Sync signal 15 pin male D shell connector used with synchronization signal cable J10 Sync signal 9 pin female D shell connector used with synchronization signal cable For more information about the connections see chapter Cables AC Power The Agilent 325 UV VIS Dual Wavelength Detector may be connected to any voltage in the range 100 24
37. e gt Create Instrument Create Refresh Edit Notification Columns Create instrument RE Create Location Instruments Ef instruments X Administration Your startup license expires in 60 days 2 Define the Name Example PrepLC 110 Purification Solution System User Guide Installation 3 m r lition Contro Management Q wstruments and Locabons Properties Name Description Appicaton instrument controler instrument type Contact 0G Wstruments SK Administration Your startup license expires in 60 days 4 Select Configure Instrument ro 7x ah X Edit Delete Refresh Lock Create Configure Edit Notifications Shortcuts Instrument Instruments and Locations Navigation PrepLc Not Connected EEEE Instruments Start Instrument J Prep EE instruments K Administration Your startup license expires in 60 days Purification Solution System User Guide 111 3 Installation 5 Select No to avoid the auto configuration It doesn t work with this version Otora Agilent LC System Z 20 weer evehaton J Enable rtobgert Repotng 2 Low Plow HP Sampler There is ne configuration salable for thes instrument De you want to i My wse ceotqurest Soom m Aro Ye a2 Aak tor configuration change at ChemStaton statue Ato Cortigraon she o toa ter Empty configuration N kI x Cancel Hep 6 The left panel of the Configurable Modules co
38. e Detector The detector is set up at the factory with a BOOTP or DHCP IP address If need be this can be changed by a trained service engineer who will own a copy of the diagnostics software Purification Solution System User Guide 107 3 Installation Setup the Fraction Collector The Fraction Collector is ready to be set up in OpenLab and does not need to have the hardware set up 108 Purification Solution System User Guide Installation 3 Install Agilent OpenLAB CDS ChemStation PrepLC Drivers Parts required p n Description M8301AA LC Core SW M8500AA M8500AA LC driver G9287 60001 PrepLC Drivers Hardware required Computer with OpenLAB CDS ChemStation Edition installed For details refer to the OpenLAB WorkStation Installation Guide 1 Copy AgilentDriversPrepLCChemstationSet_up msi from CD G9287 60001 to your computer 2 Doubleclick on AgilentDriversPrepLCChemstationSet_up msi and follow the steps in the setup wizard Purification Solution System User Guide 109 3 Installation Setup the System with OpenLAB CDS ChemStation Edition Control Panel Setup the System with OpenLAB CDS ChemStation Edition Control Panel Preparations e Latest driver package installed Modules are wired as described in Cable Connections on page 129 All modules are setup as described in Setup the Hardware on page 104 All modules are switched on OpenLAB configuration 1 Open the Agilent OpenLAB Control Panel and select Creat
39. e Pump ID and Pump Head Size When Using HPLC Control Software on page 67 or Setting the Pump ID and Pump Head Size When Using an Agilent 218 Pump as a Master Controller on page 68 Purification Solution System User Guide Installation 3 Pressure Module Installation Tools required Each Agilent 218 Pump can have a pressure module installed in the panel on the right side of the pump The pressure module dampens pump pulsations and supplies the current pressure value to the Agilent 218 software The pump needs this information to implement compressibility compensation and flow rate accuracy corrections and to ensure that system pressure is within the limits entered during setup Four pressure module ratings are available 60 0 MPa 600 bar 8700 psi 41 4 MPa 414 bar 6000 psi e 27 6 MPa 276 bar 4000 psi 13 8 MPa 138 bar 2000 psi 8 3 MPa 83 bar 1200 psi These have different flow rate max min see Agilent 218 Solvent Delivery Module User Manual p n G9300 90000 The pressure module is identified on the front of its panel The connector from the pressure module should be plugged into the master pump Only one pump in the HPLC system needs to have a pressure module installed Description Phillips head screwdriver You do not need to remove the top cover to install the pressure module or mixer The covers are removed in some photographs for clarity If you are using an HPLC system with a Agilent 218 as co
40. e end of the power cable into the module both the switch and the power socket are located on the connection panel on the pillar and the other into the mains power outlet In some countries it may be necessary to fit a suitable three pin power plug to the cord A three pin earthed power outlet must be used Ensure the module is always connected to the mains supply protective earth 3 Turn on the module The module will go through initialization tests and set the probe position If the initialization process is not successful refer to chapter Troubleshooting and Diagnostics in Agilent 440 Fraction Collector User Manual p n G9340 90000 If the fraction collector does not start up check each fuse as described in chapter maintenance in Agilent 440 Fraction Collector User Manual p n G9340 90000 During the initialization sequence 1 The probe rises to the full extreme of the Z axis 2 The probe travels to the full extremes of the X axis and rotates to the full extremes of the Theta axis 3 Then the probe is positioned at the front left of the fraction collector Instrument Communication Port The instrument communication port see Figure 24 on page 101 is used to connect the Agilent 440 Fraction Collector G9340A to the computer or MIB controlling the instrument Auxiliary Communication Port The auxiliary communications port provided on the Agilent 440 Fraction Collector G9340A is not used with current Agilent softwar
41. e or hardware Purification Solution System User Guide Installation 3 Connecting the Fraction Collector to the HPLC System For details on setting up the fraction collector to the HPLC System see Setup the System with OpenLAB CDS ChemStation Edition Control Panel on page 110 Determining the Volume Delay 1 For information refer to the help of the control software Determining the Correct Probe Depth 1 For information refer to the help of the control software Purification Solution System User Guide 103 3 Installation Setup Hardware Setup the Hardware This is done on the modules regardless of whether you are using HPLC control software or using one of the pumps as a master controller including following modules e Different pumps e 325 UV VIS Dual WL Detector e 440 Fraction Collector and e 410 Autosampler 104 Purification Solution System User Guide Installation 3 Setup Hardware Setup the Pump NOTE The correct setup of the pump depends on the pump head installed Choices are 5 10 25 50 100 and 200 mL min 10P 25P 50P 100P The P designation stands for PEEK The compressibility compensation for PEEK heads is different than for stainless steel or titanium heads Setup Pump ID for Pump 1 1 Turn the module on 2 Press on the Pump keyboard Setup and then Enter 3 With the left arrow key select ID of the Pump 1 HDSZ Pump head xx depends on what pump head is installe
42. e rack 1 Follow the manufacturer s instructions to assemble the sample racks 2 If needed Insert the overlay by placing the overlay over the top of the rack 98 Purification Solution System User Guide Installation Installing the Sample Racks 1 Standard racks Fit the pegs on the rack into the holes on the rack location mat The rack closest to the pillar of the fraction collector is considered rack number one OR Autosampler rack Type 128 or Type 200 Slide the extended portion on the bottom of the rack into the long slit on the rack location mat _ Figure 22 Rack location mat Cut outs on the rack location mat Holes for regular tube racks Holes for the autosampler rack Purification Solution System User Guide 99 3 3 Installation Rack Orientation 1 Place the rack in the correct orientation to get your samples in the desired order The following image shows an example of where the first sample is delivered to and the orientation of the regular racks provided with the fraction collector First sample dispensed in this position rack 1 tube 1 Rear of the fraction ele next to the 50000000000 O pillar OOO0OO0O00000000 OO0OO0O000000000 OOOOQOQ00Q000000 OOO0O000000000 OO0O0000000000 OOO0OO000000000 Front of the fraction collector Figure 23 Tube positions on the fraction collector 100 Purification Solution System User Guide Installation 3 Connecting t
43. e than 4 program lines Multi reagent addition is also possible two large volume reagent vials are available on the sample tray Service Autosampler Low instrument down time is accomplished by a high Mean Time Between Failure and quick instrument service Special attention has been paid to these aspects of the concept as is illustrated by the injection valve The Agilent 410 Autosampler will alert you when the lifetime of the seal is exceeded or if the switching torque becomes too high This allows preventive maintenance before injection performance degrades And if necessary the entire injection valve can be replaced in seconds with the unique Quick fit valve mounting mechanism Purification Solution System User Guide 13 1 Introduction Autosampler G9332A By just choosing PREP in your system settings you can use the Agilent 410 Autosampler to inject all of your sample into a Preparative LC system or in other areas where large injection volumes are required The combination of large sample vials 10 mL a large sample volume needle and a 2 5 mL syringe enable you to inject large volumes very reproducible with high speeds and only 45 uL of sample loss The installed large bore valve 0 75 mm with 10 mL sample loop enables you to inject from microliters to milliliters with the same AutoSampler Flow rates up to 200 mL per minute are possible when in the Prep mode Table 1 Tubing of the Agilent 410 Autosampler prep option Materia
44. eady in Enable ready in Dac ne m ok Cart Help 0K cance Hep n ee 3 Verify the connection by clicking on Activate communication Information Communication established with COM1 is visible on the screen 4 Click OK Configuration dialog closes Purification Solution System User Guide 115 3 116 Installation Fraction Collector Configuration 1 Doubleclick in Configurable Modules on Fraction Detector Fraction Collector Configuration dialog opens 2 Select the COM Port as COM 2 r Fraction Collector Configuration Instrument 1 COM Pot COM2 X Activate communication Serial number AY1101M015 Flow settings Volume delay 30 0s Rack and tube settings Probe depth 5mm Max tube time 1 00 min i Communication established with COM2 OK Cancel e Help 4 3 Fill in the Flow settings and the Rack and tube settings 4 Verify the connection by clicking on Activate communication 5 Verify the connection by clicking on Activate communication Information Communication established with COM2 is visible on the screen Purification Solution System User Guide Installation Setup the System with OpenLAB CDS ChemStation Edition Control Panel Terminating configuration 1 Before closing the Configure Instrument windows deselect the 3D spectral evaluation check box Selected Modes Agitent Prep iC Ato
45. ed input signal will start the active method if the Agilent 325 Detector is in the Ready state This polarized output signal will be activated for 600 ms when the Agilent 325 Detector starts a time program This polarized edge triggered input signal informs the Agilent 325 Detector that a fault condition exists in another instrument in the system The Agilent 325 Detector halts the time program and sends a Fault Out signal The lamp can be programmed to either remain on or turn off upon receiving a fault signal This polarized output signal will activate for 600 ms when either of the following conditions occurs The Agilent 325 Detector discovers an internal fault condition that warrants aborting the run The Agilent 325 Detector receives a Fault In signal and it has no internal fault condition itself This edge triggered contact closure causes an auto zero adjustment This edge triggered contact closure switches the lamp off It is possible to turn the lamp back on manually if the contact is still closed An optional Assy PWB sync interface 325 p n 210186590 is available to interface between the Agilent 325 UV VIS Dual Wavelength Detector synchronization signals and other devices This board is inserted into the P9 and J10 connectors and connects to a terminal strip on the adapter board This terminal strip accepts bare wire leads from cables connecting other devices These cables may originate from the other device or a dedicated
46. ements of the Electromagnetic Compatibility EMC Directive and the Low Voltage electrical safety Directive commonly referred to as the LVD of the European Union Agilent has confirmed that each product complies with the relevant Directives by testing a prototype against the prescribed EN European Norm standards Proof that a product complies with these directives is indicated by e the CE Marking appearing on the rear of the product and e the documentation package that accompanies the product containing a copy of the Declaration of Conformity The Declaration of Conformity is the legal declaration by Agilent that the product complies with the directives listed above and shows the EN standards to which the product was tested to demonstrate compliance Purification Solution System User Guide 151 6 Appendix Electromagnetic Compatibility 152 EN55011 CISPR11 Group 1 ISM equipment group 1 contains all ISM equipment in which there is intentionally generated and or used conductively coupled radio frequency energy which is necessary for the internal functioning of the equipment itself Class A equipment is equipment suitable for use in all establishments other than domestic and those directly connected to a low voltage power supply network which supplies buildings used for domestic purposes This device complies with the requirements of CISPR11 Group 1 Class A as radiation professional equipment Therefore there may be poten
47. en the affected fitting just enough to stop the leak Connecting Communication Cabling Using Serial Interface On the rear panel there is a single RS 422 male connector for serial communications Communications between slave pumps and controller a computer or master pump are established through the serial interface channel This bi directional communication protocol uses the EIA RS 422 RS 485 interface specification for data transmission Internal software in the pump determines whether the pump is a master controller or a slave pump One software controlled connector located on the back panel sends and receives signals from the controller master pump or external computer This connector is used on all pumps both when the pump Purification Solution System User Guide 63 3 64 Installation is used as a controller or as a slave the software informs the controller of the status of each pump The various configurations are discussed below Connection with a computer is covered in the interface manual of the specific software system used Using OpenLAB as the System Controller 1 Cable your system see Cable Connections on page 129 2 Go to Setting the Pump ID and Pump Head Size When Using HPLC Control Software on page 67 or Setting the Pump ID and Pump Head Size When Using an Agilent 218 Pump as a Master Controller on page 68 to set the pump ID and pump head size Using a Agilent 218 Pump as a System Controll
48. er 1 Enter the ID number of each Agilent 218 Pump using its own keypad Each pump in the system is connected with the serial interface cable To distinguish one module from another each module has a unique ID number This number must be entered in each Agilent 218 Pump using its own keypad The ID number of the master pump is set by the user to MC Slave pump ID numbers can be set as desired Each system can have only one master pump Slave pump IDs can also be set from a master pump from the BUS IDs menu Purification Solution System User Guide Installation 3 Installing the Solvent Delivery Module External Contacts Connectors J3 J2 INJECT Figure 13 External contacts connector The Agilent 218 Pump has a number of analog and digital connections on the rear panel These can be used to digitize data from an analog detector start and stop other devices through contact closures and to receive contact closures to start and stop the pump Connections are made to this strip by two connectors included in Standard accessory kit p n 393550991 These two connectors are then attached to another device Purification Solution System User Guide 65 3 66 Installation Table5 J3 terminal strip Name Connection INTGR HI Positive wire from detector INTGR LO Negative wire from detector INTGR GND Ground wire from detector CHASSIS GND Ground wire to chassis ANALOG OUT 0 10 V output signal Specifies which optio
49. evant Material Safety Data Sheets MSDS gt Be sure to handle each solvent properly gt Wear proper personal protective clothes e g safety goggles so that a solvent will not come into direct contact with the skin gt Ventilate the laboratory room adequately to prevent accidental inhalation of harmful solvent vapor Cuts gt When working with glass or quartz parts take care to prevent breakage 144 Other Precautions Airflow to the cooling fans of the liquid chromatograph must be unobstructed Do not block the ventilation grills on the liquid chromatograph and accessories Consult the manuals supplied with your PC monitor and for their specific ventilation requirements Purification Solution System User Guide Appendix 6 High Pressure Hazards WARNING High velocity stream of volatile and or toxic liquids If a line ruptures a relief device opens or a valve opens accidentally under pressure potentially hazardous high liquid pressures can be generated by the pump gt Wear personal protective equipment when you inject samples or perform routine maintenance gt Never open a solvent line or valve under pressure Stop the pump first and let the pressure drop to zero gt Always keep the doors and covers closed during operation gt Read and adhere to all Notes Cautions and Warnings in the manual Ultraviolet Radiation WARNING Irritation to the skin eyes and upper respiratory sy
50. gt Always use a fork lift or other suitable lifting device when moving the instrument CAUTION Overheating of the pump Objects interfering with airflow to the pump gt Maintain at least 15 cm 6 inches clear space next to the fan 30 Purification Solution System User Guide Installation 3 Unpacking the Detector Ensure there is enough room on the bench for the detector Heavy weight The Agilent 325 UV VIS Dual Wavelength Detector weighs in excess of 15 kg 33 Ib gt Carry the instrument at least with 2 people gt Avoid back strain or injury by following all precautions for lifting heavy objects gt Ensure that the load is as close to your body as possible gt Ensure that you can cope with the weight of your load 1 Carefully unpack the unit from the shipping carton and place it on the bench 2 Make sure to check carefully for all miscellaneous components that might be contained in the inner compartments The detector is a sensitive instrument and should always be handled with the degree of care appropriate for laboratory instrumentation Keep the shipping carton as it provides excellent protection if you have to transport or store the detector in the future Purification Solution System User Guide 31 3 Installation Unpacking the Autosampler CAUTION Risk of damaging the autosampler gt Do not lift the Agilent 410 Autosampler by the front cover 1 Lift the Agilent 410 Autosampler as show
51. he Power to the Fraction Collector The connection panel of the Agilent 440 Fraction Collector G9340A is located on the pillar also called a control column The panel contains an I O port indicators DIP switches the power socket and switch and a RS 232 port for communication between the Agilent 440 Fraction Collector G9340A and the system or computer that is running instrument control software Error indicators 1234 Error unun Reset button Dip switches DIP A OW A A 70 port Relay status indicators showing the status of relays A B and 0 which are used forthe hardwired 1 0 circuit 0 rd Console 3 software configuration anun Status indicators ei Auxilliary zi a ommunication port fnot supported S Instrument mj communication gt port o Power suppl inlet switch and fuses Figure 24 Connection panel Three power cables are supplied with the module Select the correct one for your location WARNING Electrical shock Electrical power for the module must be provided through a three wire outlet with ground connection The outlet must be rated for at least 75 VA gt Ensure that power receptacles are earth grounded at the grounding pin Purification Solution System User Guide 101 3 102 Installation Ensure that the probe arm s movement is not interfered with during operation initialization 1 Ensure that the power switch is turned off 0 2 Plug on
52. he mixer compartment The flat sides of the connector should be toward the rear of the pump OS Figure 6 Mixer internal connections 4 Fitthe mixer panel onto the module and use the screws to fasten it to the pump Purification Solution System User Guide 45 3 46 Installation Mast Kit and Components Installation 1 2 3 4 5 Installing the Mast on page 47 Installing the Manual Injection Valve and Bracket on page 48 Installing the Prime Purge Valve and Bracket_system on page 50 Installing the 3 way Pump Head Prime Valve on page 51 Installing the Column Hanger on page 53 Purification Solution System User Guide Installation 3 Installing the Mast Parts required Description Mast kit 2x mast clamp bracket assembly 2x 10 32 socket scres stainless steel mast 1 Remove the mast brackets and two screws from the Mast Kit Assembly 2 Attach a mast clamp to the fittings at the front corner of the pump OR Attach a mast clamp to the fittings one each on the lower front corner of the pump and top front corner of the detector if fitted 3 Secure the stainless steel mast within the clamps so the lower end of the mast is level with the bench Purification Solution System User Guide 47 3 Installation Installing the Manual Injection Valve and Bracket Tools required Description Hex key 2 mm provided with the injection valve Parts required p n Description In
53. hich is the small hook at the top of the Z axis slider Allow for a small length in the tubing to prevent kinking the tubing 10 Run the tubing along the back side of the probe arm housing 96 Purification Solution System User Guide Installation 3 Installing the Fraction Collector 11 Attach the split ring also called the retaining ring as shown below The retaining ring is split to attach it through the loop on the underside of the probe arm housing ee rT Split ring retaining ring 12 Feed the tubing through the retaining loop attached to the underside of the housing 13 Screw the tubing fitting into the bottom port 3 of the valve Port 1 to waste Port 2 from detector outlet or back pressure restrictor Port 3 to the probe NOTE It is important to ensure that the length of the tubing from the probe to the valve allows the probe arm to move freely in all axes If it is too tight it will restrict movement and may cause movement failure If it is too loose the probe arm may become tangled in the tubing as it moves Tubing length between the valve and the detector depends on fraction collector placement When choosing the length of tubing ensure it is long enough to allow free movement of the arm but not too long to delay sample delivery into the tubes If the tubing is too long between the detector output and the fraction collector probe end your samples may not correspond correctly to the fraction col
54. ide 87 3 Installation Rinsing the System with Wash Solvent Tools required OR CAUTION p n Description Distilled water Distilled water lsopropanol Helium Helium Ultrasonic bath Damage to the system Crystals from salts or buffer solutions may block or damage the system gt Never use salts or buffer solutions as wash solvents gt Only use water organic solvents 88 ao oO A WwW Place a clean bottle for the wash solvent at the left hand side of the autosampler Use a mixture of distilled water and isopropanol 80 20 v v or the mobile phase as wash solvent Degas the wash solvent with Helium or an ultrasonic bath Put the wash solvent tubing in the filled was solvent bottle Press MAINTENANCE to go the autosampler maintenance functions Fill the tubing using the autosampler soft function keys SYR END and SYR HOME With SYR END a syringe volume of wash solvent is aspirated from the wash solvent bottle and the wash solvent tubing is filled With SYR HOME the syringe contents will be dispensed to the syringe waste Repeat this action until the wash solvent tubing and the syringe are completely filled and no air bubbles appear in the syringe Press Escape to leave the maintenance screen 10 After the wash solvent tubing and the syringe are filled press WASH to perform a standard wash routine Repeat the wash routine 2 or 3 times All tubing connected to the syringe valve will be rinsed with w
55. ion Collector Purification Solution System User Guide 129 5 Cables Analog Output Analog Output For analog output signals install the optional Analog signal cable p n 110744200 into the J14 receptacle Pin designations are shown below J14 2 Channel A 4 Channel B Figure 26 Pin designation for J14 The open ends of the analog output cable have labels with the signal names Channel A Channel A and Channel B Channel B 130 Purification Solution System User Guide Cables 5 Relay Output Relay Output For time programming external events a contact closure Relay output is available To configure the Relay output install the optional Assy PWB relay interface 325 p n 210187590 into the J4 receptacle Pin designations are shown below 1 Relay 1 2 Ground 3 Relay 2 4 Relay 3 5 Ground Figure 27 Pin designation for J4 There are four general purpose output relays and one dedicated Peak relay Each output uses a DIP relay that is capable of handling 500 mA of contact current At reset or power up the output relay contacts are set to the default parameters open After loading a method they will be set as defined in the method s time 0 parameters Purification Solution System User Guide 131 5 Cables The Peak relay is software programmable for duration delay and active sense At power up the relay contact will be set to the inactive state as defined by
56. ion Symbols The following is a list of symbols that appear with warnings in this manual or on the liquid chromatograph The hazard they describe is also shown A triangular symbol indicates a warning The meanings of the symbols that may appear alongside warnings in the documentation or on the instrument itself are as follows Table 10 Warning symbols gt gt gt Broken glass Chemical Electrical hazard shock Explosion Eye hazard Fire hazard hazard gt gt gt Heavy weight Heavyweight Hot surface danger to danger to l D D 2 u Moving parts Respiratory Attention hazard 1 The symbol may be used on warning labels attached to the instrument When you see this symbol refer to the relevant operation or service manual for the correct procedure re ferred to by that warning label Purification Solution System User Guide 141 6 Appendix Table 11 Information symbols Symbol Description Mains power on Mains power off Fuse m AAA Single phase alternating current Direct current When attached to the rear of the instrument indicates that the product complies with the requirements of one or more EU directives 142 Purification Solution System User Guide Appendix 6 Solvent Hazards WARNING Explosion fire asphyxiation This instrument is not explosion proof Certain solvents may cause weakening and leaks of tubings or fitthings with possible bur
57. is process at the other end of the outlet tubing for the next device in line mixer pressure monitor or drain valve If the connection leaks when the system is pressurized tighten the nut slightly until the leak stops Connections for the outlet check valve 200 mL min Head The 200 mL min head uses 0 318 cm 1 8 in tubing and fittings on its outlet The pressure module used with the 200 mL min head also uses 0 318 cm 1 8 in tubing and fittings 100 mL min Head The pressure module comes with two 0 318 cm 1 8 in to 0 159 cm 1 16 in adapters for use with the 100 mL min head The outlet of the 100 mL min head is 0 159 cm 1 16 in tubing and fittings Refer to Figure 9 on page 60 for the appropriate outlet connections for your pump The appropriate compression fitting is in the accessories package Typically they are 4 28 male nuts and ferrules Purification Solution System User Guide 57 3 Installation Table 4 Connections for the outlet check valve Outlet valve Ferrule Tubing SST SST Ferrule Tubing Ferrule Tubing PEEK PEEK Titanium Titanium Analytical SST Compression fitting SST tubing il SST ferrule Analytical Titanium Compression fitting Compression fitting LE ales e nr ad pi PEEK tubing l Ferrule collar M Titanium tubing Titanium errule PEEK ferrule Idk _ _ over 172 bar up to 172 bar 58 Purifica
58. ive output signal must be connected to the positive input signal and the negative output to the negative input The color coding and physical design of the cable connectors ensure that correct signals and polarity are matched The outputs are optical isolators and simulate a relay contact closure when they are activated see Figure 30 on page 136 The minimum requirement for an input signal to be detected is 200 ms OUTPUT 57 Figure 30 Input output schematics 136 Purification Solution System User Guide Table 9 Signal Enable Out Ready In Ready Out Start In Start Out Fault In Fault Out Auto zero Lamp off Cables 5 Signal Description J10 and P9 Description A non polarized constantly active output a short This output can be used to activate Enable In on the next instrument When Enable Ready In is set software switch this polarized input signal must be present before the Agilent 325 Detector can go to the Ready state Specifically when the Agilent 325 Detector is in the NOT Ready Lamp On state on receiving a Ready In signal a monitor period will occur after which the Agilent 325 Detector goes to the Ready state It must stay active until the Agilent 325 Detector starts Ready In will be ignored in all other states This polarized output signal indicates that the Agilent 325 Detector is in the Ready state and is ready to start a time program This polarized edge trigger
59. jection valve R000048605 Injection valve bracket 1 Unpack the injection valve with attached black cable Selection valve grub screw Dust cover with _ needle port inside it Selction valve 2 Remove the red dust cover and gently pull out the needle port from the valve 3 Remove the selection handle from the valve by loosening the two grub screws securing it to the injection valve body using the provided 2 mm hex key Flat side of metal post 4 Remove the two screws and injection valve bracket from the accessories bag that came with the injection valve 5 Attach the bracket to the injection valve with the two screws 48 Purification Solution System User Guide Installation 3 6 Replace the selection handle and tighten the two grub screws securing the handle using the 2 mm hex key The grub screws should be flush with the flat side of the metal post Needle port Next Steps 7 Replace the needle port in the valve 8 Attach the injection valve to the mast For correct height see Isocratic System on page 34 isocratic system or Binary System on page 35 binary system Purification Solution System User Guide 49 3 Installation Installing the Prime Purge Valve and Bracket_system Parts required p n Description 1 Prime purge valve consult the documentation that came with the valve for the part number 1 CP16267 Hex key 2 mm 1 000048606 1 0000
60. ket Flat side of metal il post Holes in 3 way valve body 51 3 Installation 6 Slide the selection knob onto the 3 way valve The CAUTION engraved lines on the top of the selection knob should Damage to the fittings point to the holes in the black body of the 3 way valve The grub screw should be flush with the flat side of the metal post on the 3 way valve gt Finger tighten the nut and use the provided hex key to tighten the selection knob 5 Place the flat washer and then ridged washer on the 3 way valve Screw on the nut to secure the two washers Next Steps 7 Secure the selection knob to the 3 way valve using only the provided 2 mm hex key Do not over tighten 8 Attach the 3 way valve to the mast See Table 2 on page 34 for the correct height If you have a binary system repeat this procedure for the second 3 way pump head prime valve For correct height see Table 3 on page 35 52 Purification Solution System User Guide Installation 3 Installing the Column Hanger Parts required p n Description 1 R000048610 Analytical column hanger for 1 4 in OD OR 1 R000048602 Preparative column hanger for 1 in OD OR 1 R000048601 Preparative column hanger for in OD 1 Attach the column hanger on to the mast so that the column inlet is close to the injection valve Avoid blocking the other components 2 Secure the column hanger by tightening the black thumbscrew on the bracket
61. l Dimensions Volume Tubing LSV sample needle Stainless Steel 70 mm x 0 81 mm o d x 0 51 mm i d 45 uL and tubing Tefzel 155 mm x 1 16 o d x 0 50 mm i d 45 uL Buffer tubing from PTFE 2550 mm x 1 16 o d x 1 mm i d 2000 pL high pressure valve to syringe valve 14 If the Prep option is factory installed the installation instructions can be skipped If the Prep option is bought as a kit carry out the installation instructions see Installing the Agilent 410 Autosampler Prep Option on page 91 for more information Purification Solution System User Guide Introduction 1 Introduction to the Fraction Collector The Agilent 440 Fraction Collector is a random access single probe fraction collector and can accommodate a variety of racks It is designed to automate the sample collection process This fraction collector is designed to meet the diverse requirements of high throughput laboratories Three racks are included with the fraction collector and are made of polypropylene to resist most chemical spills Up to three racks of many configurations can be placed on the fraction collector Additional sample racks can be set up in sequence and manually changed during an analysis as each rack s analysis is completed The rack closest to the rear of the fraction collector next to the pillar is considered rack number one Purification Solution System User Guide 15 1 Introduction Introduction to the Fraction Collec
62. l form and transmit the data to a computer system Five programmable analog inputs and three programmable relay outputs are available to further automate the HPLC system The Agilent 218 Solvent Delivery Module is easy to use and very flexible in operation It can be used in several different modes of operation as a standalone isocratic pump as either a master pump or a slave pump ina high pressure gradient system as a sample inject pump in a preparative system or in a fully automated HPLC system controlled by an external computer In each case the Agilent 218 Solvent Delivery Module provides outstanding accuracy over its entire range of pressures flow rates and solvents The Agilent 218 Solvent Delivery Module operates very quietly because of minimal motor noise and resonance vibrations A complete Agilent 218 Solvent Delivery Module includes a drive module a pump head and a pressure module One of the Agilent 218 Solvent Delivery Modules in the HPLC system needs to have a pressure module installed in its compartment in the pump side panel The pressure module dampens pulsations and supplies the current system pressure value to the drive module Software in the drive module ensures that the system pressure is within pre set maximum and minimum limits Flow rates are automatically corrected for solvent compression effects based on the system pressure value read from the pressure module and a compressibility factor entered by the user for e
63. lector markers on your chromatogram The delay volume calculation will compensate for this effect 14 Move the probe arm to the front right side of the fraction collector and rotate the probe arm fully to the right to check that the tubing is completely free to move 15 Manually position the probe arm in the middle along the X axis 16 Rotate the probe arm through its full extent to check that the tubing is free to allow full movement in all directions Purification Solution System User Guide 97 3 Installation If the length is not correct remove and then replace the tubing install the fittings and tubing onto the fraction collector Repeat step 14 on page 97 step 16 on page 97 to test the tubing length 17 Adjust the length of the sample line from the HPLC system In port 2 see Figure on page 97 screw in the fitting with Tefzel tubing Connect the other end of this tubing to the HPLC system s detector outlet or to the back pressure restrictor if one is present This tubing should be as short as possible 18 In port 1 see Figure on page 97 screw in the fitting with Teflon tubing Put the other end of the tubing into your waste container Assembling the Sample Racks CAUTION Loss of sample If a tube sits at an angle in the rack the probe may hit the side of the tube as it enters or leaves the tube This can affect the alignment of the probe and or damage the tube gt Ensure that the tubes sit vertically within the sampl
64. led ready for operation on 220 230 V You will need to reconfigure the voltage for 115 V usage gt Check that the voltage configuration is set correctly for your local power requirements gt If it is necessary to change the voltage configuration see the Agilent 218 Pump Voltage Conversion Instruction p n 8510249500 1 Setup electrical connections 2 Check that the ON OFF power switch is off in the O position 3 Connect the power cord to the back panel of the module and plug it into a grounded power socket A good ground connection is necessary to ensure safety for users and proper communications 4 Turn on the power switch Purification Solution System User Guide 37 3 Installation If the pump does not start check following items 1 Proper connection of the power cord 2 Power at the wall receptacle 3 Functionality of the main power fuse F1 For fuse installation see maintenance procedure in Agilent 218 Solvent Delivery Module User Manual p n G9300 90000 The fuse rating and operating voltage is printed on the rear panel next to the power receptacle 38 Purification Solution System User Guide Installation 3 Installing the Solvent Delivery Module Pump Head Installation Pump head Pump head clamp Thumbscrew or hexscrew Tools required NOTE The pumps are shipped without the pump head installed You will have to install the pump head before beginning to run You can also change pump heads at
65. n G9331 90000 Agilent 440 Fraction Collector User Manual p n G9340 90000 Purification Solution System User Guide This chapter provides information on cables used with the instrument Purification Solution System User Guide 5 Cables Cable Overview 128 Cable Connections 129 Analog Output 130 Relay Output 131 Desktop PC Communications 134 Synchronization Signals 135 ee Agilent Technologies 127 5 Cables Cable Overview Necessary cables p n Description 392612901 Ethernet cable for use in a network 5023 0203 Ethernet cable cross over for standalone use 392607969 Inject marker cable 392607975 Next injection cable 393546291 Serial communication ribbon 393597601 Converter RS232 to RS422 7910046300 Serial cable Optional cables p n Description 110743800 Relay interface cable for relay interface board one relay contact per cable 110744200 Analog signal cable 128 Purification Solution System User Guide Cables 5 Cable Connections Cable Connections Back connectors for 218 to internal CIM 9 Pin connectors Out Gao Serial cable communication a Converter RS 232 i O E Ethernet GPIB mi at Next injection 218 Pump 410 Autosampler S 325 Detector e Inject marker cable 440 Fraction lel U cPu Figure 25 Cable connections for workstation control of Agilent 218 Pumps Agilent 325 Detector Agilent 410 Autosampler and Agilent 440 Fract
66. n in Figure 1 on page 32 with both hands under the instrument or with one hand under the front and the other hand grasping the rear top of the Agilent 410 Autosampler Figure 1 Agilent 410 Autosampler lifting instructions 32 Purification Solution System User Guide Installation 3 Optimizing the Stack Configuration This section contains information on how to stack your modules depending on the preparative method For optimal performance in other configurations than in the given examples respect the following rules e The orientation is also suggested if you are only installing the Agilent 218 Pump If an autosampler is present it should be placed on top of the detector e Position the master pump as highest pump in the stack The pump with the pressure module is the master pump In gradient systems the remaining pumps are slave pumps This position allows easy access to keypad and display to control the HPLC system via pump instead of software Purification Solution System User Guide 33 3 Installation Optimizing the Stack Configuration Isocratic System Detector Injection valve Prime purge valve Low pressure solvent valve P Pressure module Figure 2 Isocratic System Table2 Isocratic system component heights on the mast measured from the bench to the bottom of the bracket Part Height in mm in Injection valve 285 11 2 Prime purge valve 185 7 3 Low pressure solvent valve 10
67. n to output as an analog signal to a recording device Programmable options are A B C D solvent composition pressure system pressure nm wavelength specified in the 1 0 window flow system flow rate or off Full scale voltage is 10 V ANALOG OUT GND Ground wire for Analog Out 1 2 3 Contact closure relay outputs These can be used to start external devices such as an autosampler 1 Do not connect to Chassis Gnd Table6 J2 terminal strip Name AUX 5V AUX GND STOP D GND HOLD D GND TRANSFER D GND Connection 5 V positive signal Ground for auxiliary voltage Contact closure input to stop the pump from an external device Digital ground for Stop signal Contact closure input from an external device to Hold a running method at the current time and flow composition conditions Digital ground for Hold signal Contact closure input to Transfer to another method from an external device Transfer can be immediate deferred until the end of the current method pass or automatic at the end of run if no contact closure is received Digital ground for Transfer signal Purification Solution System User Guide Installation 3 Table6 J2 terminal strip Name Connection INJECT Contact closure input from an external device which cancels a programmed Inject Wait or Hold D GND Digital ground for Inject signal MARK Contact closure input to perform an Event Mark a 10
68. ng shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions Purification Solution System User Guide 27 3 Installation Check Delivery 28 Delivery Checklists Delivery Checklist Pump The pump is packed in a single carton The pressure module ordered with the pump is shipped separately and needs to be installed Any pump head ordered with the pump is packed separately The following list shows all items delivered in a standard delivery Your personal list depends on your order therefore countercheck delivery with your order e Agilent 218 Solvent Delivery Module e Power cord e Pump head kit e Plumbing kit G9300A Pressure transducer module G9300A e Mast kit G9301A e Internal mixer G9301A Delivery Checklist Detector The following list shows all items delivered in a standard delivery Your personal list depends on your order therefore countercheck delivery with your order Agilent 325 UV VIS Dual Wavelength detector e Assy PWB Sync Interface 325 335 e Cross over Ethernet cable Power cord Flow cells are required for the detector but are ordered separately
69. nspection 30 Optimizing the Stack Configuration 33 Isocratic System 34 Binary System 35 Auto Preparative System 36 Installing the Solvent Delivery Module 37 Electrical Setup 37 Pump Head Installation 39 Pressure Module Installation 41 Internal Mixer Installation 44 Mast Kit and Components Installation 46 Installing the Mast 47 Installing the Manual Injection Valve and Bracket 48 Installing the Prime Purge Valve and Bracket_system 50 Installing the 3 way Pump Head Prime Valve 51 Installing the Column Hanger 53 Plumbing Connections 53 Pump Head Low Pressure Tubing and Inlet Filter Assembly 54 Installing the Piston washing Tubing 55 Pump Head High Pressure Tubing 56 Narrowbore and Analytical Stainless Steel Mixers Plumbing Fittings 61 Analytical PEEK and Narrowbore Analytical Titanium Mixers Plumbing Fittings 62 Pee Agilent Technologies 23 3 24 Installation Preparative PEEK Titanium Mixers Plumbing Fittings 63 Connecting Communication Cabling Using Serial Interface 63 Setting the Pump ID and Pump Head Size When Using HPLC Control Software 67 Setting the Pump ID and Pump Head Size When Using an Agilent 218 Pump as a Master Controller 68 Installing the Detector 69 Location of the Detector Module 69 Power Connection and Rear Panel Services 70 Avoiding Harmful interferences to Radio or Television Reception 72 Removing the Front Panel 73 Hydraulic Connections Flowcells 74 Installing a Flowcell 75 Detector Outlet Back
70. nt Technologies on Internet 154 Purification Solution System User Guide Contents 6 Purification Solution System User Guide Purification Solution System User Guide ee 1 66 Introduction e E e j Introduction to the System 8 Introduction to the Solvent Delivery Module 9 Introduction to the Detector 11 Introduction to the Autosampler 12 Autosampler G9331A 12 Autosampler G9332A 14 Introduction to the Fraction Collector 15 This chapter introduces to the Agilent 218 Purification Solution system and its components Agg Agilent Technologies 1 introduction Introduction to the System A complete Agilent 218 Purification Solution system includes e Agilent 218 Solvent Delivery Module s e Tubing e Mast kit e Agilent 325 UV VIS Dual WL Detector e Agilent 410 Autosampler and e Agilent 440 Fraction Collector 8 Purification Solution System User Guide Introduction 1 Introduction to the Solvent Delivery Module The Agilent 218 Solvent Delivery Module uses proven single piston rapid refill technology for economy reliability and virtually pulse free operation A range of interchangeable pump heads allows operation at flow rates from 10 uL min to 200 mL min Biocompatible pump heads are available for those analysts requiring a completely inert flow path A single channel analog to digital converter built in to each Agilent 218 Solvent Delivery Module can convert a detector signal to digita
71. ntains the Agilent Prep LC Ortens Agilent LC System Use classe devers V 2D spect evatuaton Enable tekoart Rrpoting Aak for confiqueition change at ChamStaton startup AdStional configuration x Orce Hep 7 Select the module you have available in your system 8 Click gt to add the modules to the Selected Modules The configuration dialog for the selected module opens 112 Purification Solution System User Guide Installation Pump Configuration 1 Doubleclick in Configurable Modules on Pump Pump Configuration dialog opens 2 Select the COM Port as COM 1 and the desired Pressure unit bar available kPa PSI bar MPa Pump Configuration Instrument 1 COM Port com X Number of pumps Pressure unit bar Z E Injection pump present Pump ID Model Firmware Head version A 1 PrepStar218 HPXLV2 0SD300V1 1 25mL Titanium OK cma tee 3 Verify the connection by clicking on Activate communication Information Communication established with COM1 is visible on the screen 4 Click OK Configuration dialog closes Purification Solution System User Guide 113 3 3 Installation Autosampler configuration 1 Doubleclick in Configurable Modules on Autosampler Autosampler Configuration dialog opens 2 Select the COM Port as COM 1 F a Autosampler Configuration Instrument 1 COM Port COM1
72. ntroller the pump with the pressure module should be the master controller device ID MC For the Agilent Modular HPLC Systems the pressure module must go into the topmost pump Purification Solution System User Guide 41 3 Installation Installing the Solvent Delivery Module NOTE If you are using a computer as controller the pump with the pressure module needs to be identified in the controller configuration window 1 Decide which pump is to have the pressure module NOTE installed i e which pump is the master pump then remove the panel on the rear of the right side using a Phillips head screwdriver Keep the panel in a safe place 42 Purification Solution System User Guide Installation Installing the Solvent Delivery Module 3 2 Place the pressure module in position and attach the connector to the plug on the inside of the pressure module compartment The connection is done using a 9 pin D shell connector The 4 pin side of the connector is positioned towards the rear of the pump module The module and its connector are a one way fit Mixer connection with flat side toward rear of connector D shell connector with 4 pin side toward back of module Pressure module connector Next Steps 3 Tighten the screws on the mounting panel to hold the module in place This is critical as the pressure module can slip out if the pump is picked up without the scre
73. of the Module 2 10 11 9 Figure 20 Fraction collector components 1 Theta axis Z axis slide 3 Top knurled mount nut on the probe assembly 4 Probe mounting block 5 Lower knurled mount nut on the probe assembly 92 Purification Solution System User Guide Installation 3 6 Nut holding the metal probe 7 Probe 8 Rack 9 Rack location mat 10 Split retaining ring 11 Fraction collector pillar control column Installation Overview Basic installation involves e Assembling the fraction collector Connecting the fraction collector to the HPLC system Installing Agilent OpenLAB CDS ChemStation PrepLC Drivers e Determining the delay volume and probe depth All external DIP switches should always be in the OFF position Assembling the Fraction Collector Carefully follow the instructions provided below to assemble your instrument Assembling the Agilent 440 Fraction Collector includes installing the 1 Spill tray 2 Rack location mat 3 Probe and tubing 4 Sample racks Position the fraction collector on the side of the HPLC system closest to the detector output to help decrease the delay volume Purification Solution System User Guide 93 3 Installation Installing the Fraction Collector Installing the Spill Tray Alignement pin underneath the raised location tabs Back side of the fraction collector closest to the fraction Figure 21 Spill tray alignment pin and mat for the rack loca
74. onnection Tubing 3 Remove the front panel see Removing the Front NOTE i Panel on page 73 for more information The position of the inlet and outlet connections varies for different flowcells 76 Purification Solution System User Guide Installation 3 4 Carefully position the flowcell so that the two thumbscrews are positioned in the threaded holes in the flowcell compartment a Push at the center of the flowcell to ensure it is positioned squarely in its housing and that it is not tilted in any way It is critical to the performance of the detector that the flowcell is inserted correctly The internal optical components of the flowcell are an integral part of the detector s optical system If the flowcell is not fitted correctly it will have an adverse effect on detector performance Next Steps 5 Secure the flowcell by tightening the thumbscrews with your fingers Alternate tightening the thumbscrews until they are snug 6 Replace the front panel For optimum performance the detector should be operated with the front panel in place This is because the foam on the inside of the panel stops breezes which may cause instability and noise from reaching the flowcell The flowcell should be removed with the connecting tubing fitted These must be removed outside the detector compartment 7 Perform a lamp calibration see chapter maintenance in Agilent 325 UV VIS Dual Wavelength
75. rds 19 specifications 21 spill tray installation 94 stack auto preparative 36 binary 35 isocratic 34 optimizing 33 synchronization signals 135 syringe waste tubing connecting 86 system rinsing 88 T terminal strip J2 66 J3 66 troubleshooting 126 U unpacking 30 using 126 V vials filling and sealing 89 WwW waste Purification Solution System User Guide Index electrical and electronic equipment 148 water and leakage drain connecting 86 WEEE directive 148 Purification Solution System User Guide 157 www agilent com In This Book This manual provides setup information on following modules Agilent 218 Solvent Delivery Module G9300A G9301A Agilent 325 UV VIS Dual WL Detector G9309A e Agilent 410 Autosampler G9331A G9332A Agilent 440 Fraction Collector G9340A The manual describes the following e Optimizing stack configuration Setup hardware e Setup software e Setup methods Agilent Technologies 2012 Printed in Germany 02 2012 G9300 90300 EE Agilent Technologies
76. region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Purification Solution System User Guide 19 2 Site Requirements and Specifications Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation Area selected WARNING Explosion
77. sA ID 60 i Activate communication a FW version Options Tray 84 standard vias Loop volume 100 pL Syringe volume 250 pL z Tubing volume 15 pl Preparative option No V Column oven W Tray cooling V Aam buzzer i Communication established with COM1 x J Goce Jt Select the Tray84 standard vials as an example Double check your actual configured Tray Check the other variables present in the Autosampler Configuration screen ao oO Aa Ww Verify the connection by clicking on Activate communication Information Communication established with COM1 is visible on the screen 7 Click OK Configuration dialog closes 114 Purification Solution System User Guide Installation 3 Setup the System with OpenLAB CDS ChemStation Edition Control Panel Detector Configuration 1 Doubleclick in Configurable Modules on Detector Detector Configuration dialog opens 2 Select the Browse function to start discovery your Detector The IP address will be populated by the searching results Example IP address 192 168 0 97 r r Detector Configuration Instrument 1 Detector Configuration Instrument 1 a m f IP address 0 0 0 0 a IP address 192 168 0 97 g l Activate communication ____ Activate communication Options Options Dual wavelength option Enabled Dual wavelength option Enabled Cell type 0 M Cell type x0 a Enable r
78. stallation of your Agilent 218 Solvent Delivery Module Agilent 325 UV VIS Dual WL Detector Agilent 410 Autosampler and Agilent 440 Fraction Collector Purification Solution System User Guide 25 3 Installation Delivery 26 For detailed information on parts delivered with the modules refer to the corresponding module manuals e Agilent 218 Solvent Delivery Module User Manual p n G9300 90000 e Agilent 325 UV VIS Dual Wavelength Detector User Manual p n G9309 90000 e Agilent 410 Autosampler User Manual p n G9331 90000 e Agilent 440 Fraction Collector User Manual p n G9340 90000 Components of a Complete System Hardware Modules Software A complete system comprises following modules as ordered p n Description G9300A Agilent 218 lsocratic Solvent Delivery Module G9301A Agilent 218 Add On Solvent Delivery Module OPTIONAL G9309A Agilent 325 UV VIS Dual WL Detector G9331A Agilent 410 Autosampler G9332A Agilent 410 Preparative Autosampler OPTIONAL G9340A Agilent 440 Fraction Collector p n Description G9287 60001 Prep LC driver CD M8500AA LC driver M8301AA LC Core Software OpenLAB CDS A 01 03 or higher Purification Solution System User Guide Installation 3 Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged duri
79. stem gt Ensure that protective lamp covers of variable and fixed wavelength detectors are in place during operation gt Do not look directly into detector fluid cells or at the UV light source When inspecting the light source or fluid cell always use protective eye covering such as borosilicate glass or polystyrene gt Ventilate the area surrounding the detector such that the concentration of ozone does not exceed the maximum permissible level All venting must be to outside air never within the building Ozon generation Ozone can be generated by radiation from the source lamps The maximum permissible exposure level is 0 1 ppm 0 2 mg m Purification Solution System User Guide 145 6 Appendix Solvent Miscibility Solvents should mix with each other in all proportions This is important during elution and during solvent changeover Refer to Figure 32 on page 146 for miscibility of some common HPLC solvents Acetic acid Acetone HB immiscible Acetonitrile Benzene Miscible Butyl alcohol Carbon tetrachloride Chloroform Cyclohexane Cyclopentane Dichloroethane Dichloromethane Dimethylformamide Dimethylsulfoxide Dioxane Ethylacetate Ethyl alcohol Di ethylether Heptane Hexane Methyl alcohol Methylethyl ketone Iso octane Pentane Isopropyl alcohol Di Propylether Tetrachloroethane
80. sting Even small leaks in solvent supply systems can be dangerous gt Only use solvents compatible with the HPLC system tubings and fittings gt Employ static measuring and static discharge devices to safeguard against the buildup of static electricity gt In unattended operation do not use organic solvents having an ignition point below 70 C gt Do not bring a heat or flame source near the instrument gt The area in which solvents are stored and the area surrounding the instrument must be adequately ventilated to prevent accumulations of gas gt Always check the condition of the instrument leakage of solvent or waste solution leakage of solvent inside the instrument If an abnormality is found stop operation immediately gt When using flammable chemicals be careful about possible ignition due to static electricity To prevent the build up of static electricity use a conductive container for waste gt Use only approved regulator and hose connectors refer to the supplier s instructions gt Keep solvents cool and properly labeled Ensure that you have the correct solvent before connecting it to the instrument Purification Solution System User Guide 143 6 Appendix WARNING Inflammation or injury due to toxic corrosive or stimulative solvent gt Do not contact toxic corrosive or stimulative solvent gt For details of the properties of each solvent and how to handle it refer to the rel
81. t fitting to the inlet port on the pump head check valve the lower port Tighten the inlet tubing finger tight If leakage occurs in use tighten very slightly with an open end wrench until the leak stops Do not over tighten The fitting may be damaged Installation of inlet tubing for 200 mL min heads 1 Remove the solvent inlet assembly from the accessory package and immerse diagonally cut end of tubing into solvent container The solvent containers should be located on the floor with the pump on the bench 2 Connect the inlet fitting to the inlet port on the pump head check valve the lower port Tighten the inlet tubing finger tight If leakage occurs in use tighten very slightly with an open end wrench until the leak stops Do not over tighten The fitting may be damaged Installing the Piston washing Tubing Skip this step if you do not have piston washing on your pump heads The wash tubing is provided as a single piece When pumps are stacked the tubing from the upper pump rinse outlet can be connected to the rinse inlet of the lower pump so that pump heads can be rinsed in series The tubing clamp need only be attached to the outlet rinse tubing on the bottom pump 1 Cut into two pieces of appropriate length for the wash inlet and outlet 2 Thread male Luer fittings into the inlet top and outlet rinse ports and connect the silicone tubing to the Luer fittings 3 Attach the tubing clamp to the outlet tubing Purific
82. the value of the active sense parameter stored in the detector Upon being triggered relay activation will occur for the time interval equal to the Peak Sense duration parameter as stored in the method The Peak relay can be activated from any of the following sources only one source can be active at any one time Time Slice event Once time slice has been turned on it will provide a periodic activation of the Peak Sense relay at an interval defined in Time Slice period within the method Time Slice can be turned on and off by time e Pulse event A single timed programmed activation of the Peak Sense relay as defined in the method e Peak sense has been turned on To connect open ended wires to the relay signals available at J4 use the optional Assy PWB relay interface 325 p n 210187590 see Figure 28 on page 132 Simply plug this PWB into the rear panel connector 5 P Figure 28 Relay output board 132 Purification Solution System User Guide Cables 5 The Relay interface cable for relay interface board one relay contact per cable p n 110743800 is used to attach to the 3 pin connectors at the relay output board With the 3 pin connectors the contact closure is between pins 1 and 2 of the plugs Pin 3 is connected to ground The relay interface cable has three open ended wires The relay contact is connected between the clear and the black wire The green wire is connected to ground Purification
83. tial difficulties in ensuring electromagnetic compatibility in other environments due to conducted as well as radiated disturbances Operation is subject to the following two conditions e This device may not cause harmful interference This device must accept any interference received including interference that may cause undesired operation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try one or more of the following measures e Relocate the radio or antenna e Move the device away from the radio or television e Plug the device into a different electrical outlet so that the device and the radio or television are on separate electrical circuits e Make sure that all peripheral devices are also certified Make sure that appropriate cables are used to connect the device to peripheral equipment e Consult your equipment dealer Agilent Technologies or an experienced technician for assistance e Changes or modifications not expressly approved by Agilent Technologies could void the user s authority to operate the equipment Purification Solution System User Guide Appendix 6 ICES NMB 001 This ISM device complies with Canadian ICES 001 Cet appareil ISM est conforme a la norme NMB 001 du Canada Purification Solution System User Guide 153 6 Appendix Agilent Technologies on Internet
84. tion 3 Installing the Door The module door may be attached to the front of the Agilent 325 Detector to cover the tubing connections to the flowcell If the Agilent 325 Detector is the top module in the stack the door cap should be installed before installing the door on the module Installing the Door Cap If the Agilent 325 Detector is not at the top of the stack do not install the cap and proceed to the instructions describing door installation 1 Stand the door upside down on a flat surface i e with the two ribs towards the bottom Ribs Magnetic latch Door cap Figure 16 Installing the door cap 2 Take the cap and remove the protective paper exposing the adhesive that will attach the cap to the door 3 Insert the cap into the door and press the adhesive onto the inside of the door lip Be sure to keep the door edges and cap edges flush Purification Solution System User Guide 79 3 Installation Installing the Detector Installing the Door 1 Insert the top hinge pin into the top hinge 2 Gently press down on the top of the door and slide the lower hinge pin into the lower hinge The door should now pivot on the pins and close The magnetic door latch should stick to the instrument Figure 17 Installing the Agilent 325 Detector door Installing and Removing the Door 1 Gently push down on the door and slide the lower hinge pin out of the lower hinge
85. tion Solution System User Guide Installation 3 Table4 Connections for the outlet check valve Ferrule Tubing Ferrule Tubing PEEK PEEK Outlet valve Ferrule Tubing SST SST Titanium Titanium Semi Prep SST Compression fitting tubing wean over 172 bar 1 8 in tubing Semi Prep Titanium Compression fitting Compression fitting It PEEK tubing l a Titanium tubing 3 uaig 77777 l Titanium ferrule w PEEK ferrule over 172 bar up to 172 bar 1 Unit conversion 172 bar 2500 psi 17 2 MPa 2 Unit conversion 1 8 in 0 318 mm Purification Solution System User Guide 3 Installation Installing the Solvent Delivery Module Small opening at top Figure 9 Detail of PEEK ferrule and ferrule collar 60 Purification Solution System User Guide Installation 3 Narrowbore and Analytical Stainless Steel Mixers Plumbing Hw Extra long fitting and pats Inlet fitting and aa Figure 10 Compression fittings for 0 6 mL narrowbore and 1 2 mL analytical stainless steel mixers Tools required Description Wrench 1 4 in 1 For both the extra long fitting on the outlet and the two standard fittings for the inlet slide the fitting and ferrule over the 0 159 cm 1 16 in OD tubing and push the end of the tubing into the port as far as possible 2 Holding the tubing in place finger tighten the fitting then tighten 1 4 turn more with a wrench When tightening the outlet fitting
86. tion raised locating tabs Preparations Position the fraction collector closest to the detector output This decreases the delay volume NOTE The spill tray will catch most solvent spills Always clean up spills immediately 1 Locate the alignment pins protruding above the base support tube 2 Lower the tray so that the two alignment pins closest to the fraction collector pillar fit into the slots in the spill tray 3 Lower the spill tray until the front edge rests on the front of the base support tube 4 Check that the spill tray is firmly pushed down on all alignment pins NOTE The spill tray must be firmly in position so that it does not move 94 Purification Solution System User Guide Installation 3 Installing the Rack Location Mat Preparations The rack location mat is installed on top of the spill tray Spill tray installed There are cut outs around the rack location mat The cut outs slot over the raised locating tabs that are molded into the spill tray to ensure proper alignment see Figure 22 on page 99 Position the rack location mat over the spill tray and press the mat onto the locating tabs Check that the rack location mat fits firmly in place by trying to move it side to side There should not be any movement If the rack location mat is loose refit it Installing the Probe and Tubing The sample probe comes preassembled with 0 050 cm 0 020 in ID tubing There are three additional coils of tubing
87. to the Autosampler 12 Autosampler G9331A Introduction to the Autosampler The Agilent 410 Autosampler has been designed to meet the needs of the modern analytical laboratory The autosampler has the following features e Reliable e Cost effective e Easy to use e Column temperature control and sample cooling guaranting consistent results High resolution syringe control guaranting superior precision for injection and reagent addition e Fast replacement of the injection valve Loop injection with Pressure Assisted Sample Aspiration is a proven concept that combines high precision with simplicity and reliability Three injection modes can be selected e Full loop Partial loop filling e uL Pick up Therefore maximum precision maximum flexibility and zero sample loss can be achieved Side Port Needle The strong side port needle combines the optimum point style for septa piercing with a minimum risk of blockage by septum particles Purification Solution System User Guide Introduction 1 Column Oven A column oven is an integral part of the Agilent 410 Autosampler because constant column temperature is important for long term stability of a chromatographic separation and may be required for GLP compliance Reagent Addition Internal Standard addition sample dilution or derivatization can be programmed in a very simple manner A single stage derivatization of a sample in a separate destination vial requires no mor
88. tor 16 Purification Solution System User Guide Purification Solution System User Guide ee 2 Site Requirements and Specifications e i e j Site Requirements 18 Specifications 21 This chapter provides infromation on site requirements and specifications of your system gg Agilent Technologies 2 Site Requirements and Specifications Site Requirements Power Considerations The instrument power supply has wide ranging capability It accepts any line voltage in the range described in Physical Specifications Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 18 Purification Solution System User Guide Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or
89. ve units either three additional pumps in a quaternary system or two additional elution pumps and one injection pump The master Agilent 218 Solvent Delivery Module can control other modules in the system using outputs and receive information through input contacts e Gradient system with HPLC control software as a controller In this configuration all pumps are slaves and the computer is the system controller The HPLC control software controls the pumps via the serial interface cable and other devices through contact closures on the Control Interface module CIM built into the Agilent 218 Solvent Delivery Module Purification Solution System User Guide Introduction 1 Introduction to the Detector The Agilent 325 UV VIS Dual WL Detector is integrated into a Liquid Chromatography System The detector is controlled remotely by OpenLAB through Ethernet communications In this situation all functions of the detector are controlled through the Workstation software The detector measures the sample absorbance at the user selected wavelength The absorbance is displayed Wavelength absorbance parameters are time programmable Features of the Agilent 325 UV VIS Dual WL Detector e Stackable module Interchangeable flowcells Simple lamp replacement e Comfortable control OpenLAB e Wide detection range peaks up to 40 AU cm with appropriate flowcell Purification Solution System User Guide 11 1 Introduction Introduction
90. vertical trace on the chromatogram from an external device D GND Digital ground for Mark signal 1 Do not connect to Chassis Gnd Setting the Pump ID and Pump Head Size When Using HPLC Control Software This is done on the pumps whether you are using an HPLC control software or using one of the pumps as a master controller To set the pump ID when all pumps are slaves 1 Turn the pump on 2 Press SETUP and then press ID 3 Set the ID for the Agilent 218 Pump either by entering a number between 0 and 63 or pressing the UP ARROW or DOWN ARROW key to scroll through a preset list of choices Available choices are 0 63 MC master controller or no ID To set the pump head size when all pumps are slaves 1 Turn the pump on 2 Press SETUP and then press HdSz 3 Use the UP ARROW or DOWN ARROW key to select between a preset list of choices Choices are 5 10 25 50 100 and 200 mL min 10P 25P 50P 100P The P designation stands for PEEK The compressibility compensation for PEEK heads is different than for stainless steel or titanium heads Purification Solution System User Guide 67 3 68 Installation Setting the Pump ID and Pump Head Size When Using an Agilent 218 Pump as a Master Controller Setting the pump ID and pump head size 1 Turn the pump on 2 Press SETUP 3 Press PUMP 4 Select between A B C and D Selecting a pump opens a window to set Pump ID Head size compressibility factors
91. ws being tightened Purification Solution System User Guide 43 3 Installation Internal Mixer Installation If two or more pumps are being used together to proportion individual solvents into one mixture a mixer is required Mixers are available in several different materials and three different sizes gt A0 6 mL e 1 2 mL and 10 mL mixer The internal mixer is identical in function and capability to the external mixer except that it obtains power from the pump drive module and it mounts into the pressure module bay at the right rear of the pump The mixer is identified by a label on the inside of the mixer mounting panel This identifies the mixer size material and part number The mixer connects to the Agilent 218 Pump using a 2 pin Molex connector for power There is no ON OFF switch for the mixer Whenever the Agilent 218 Pump is powered on the mixer is running This continuous running does not hurt the mixer When no liquid is present the mixing bars may not be moving This is normal When liquid is present the mixing bars flip back and forth rapidly to mix the solvents 1 Ensure the pump power is turned off 2 Remove the cover panel from the right rear of the Agilent 218 Pump Save the screws ff 7 MA 44 Purification Solution System User Guide Installation 3 Installing the Solvent Delivery Module 3 Attach the 2 pin Molex connector to the white Molex connector in t
92. xer installation 44 internet 154 introduction detector 11 L lithium batteries 149 location detector module 69 low pressure tubing 54 155 Index M maintenance module 126 anual injection valve and bracket installation 48 mast kit installation 46 mat rack location 95 method setup 118 0 outlet check valve connections 57 overview installation 93 module 82 P packaging damaged 2 parts 126 piston washing installation 55 plumbing fittings narrowbore titanium 62 preparative PEEK 63 plumbing fittings analytical stainless steel 61 analytical titanium mixers 62 narrowbore 61 preparative titanium 63 plumbing safety 53 power cords 19 power connection 101 prep option 156 installing 91 using 14 pressure module installation 41 prime purge valve and bracket installation 50 pump head high pressure tubing installation 56 pump ID setting control software 67 pump head installation 39 setting control software 67 pump overview 9 rack location mat 95 rack orientation 100 radio interference 150 reagente addition 13 relay output 131 removing door 80 front panel 73 S safety information lithium batteries 149 safety general information 140 sample rack assembly 98 installation 99 sample tray installation 90 85 setup ChemStation control 110 electrical 37 hardware 104 OpenLab 110 pump D 105 side port needle 12 site requirements power co
93. y per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Purification Solution System User Guide In this book In this book This manual provides setup information on following modules e Agilent 218 Solvent Delivery Module G9300A G9301A e Agilent 325 UV VIS Dual WL Detector G9309A e Agilent 410 Autosampler G9331A G9332A e Agilent 440 Fraction Collector G9340A 1 Introduction This chapter introduces to the Agilent 218 Purification Solution system and its components 2 Site Requirements and Specifications This chapter provides infromation on site requirements and specifications of your system 3 Installation This chapter gives information about the installation of your Agilent 218 Solvent Delivery Module Agilent 325 UV VIS Dual WL Detector Agilent 410 Autosampler and Agilent 440 Fraction Collector 4 Using Troubleshooting Maintenance and Parts This chapter provides information on how to access further details on the system components 5 Cables This chapter

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