ÄKTAexplorer Installation Guide 18113959ad
Contents
1. 18 Run the installation test method 22 Evaluate the gradierit u nio lalla oi 27 e Evaluate the step response 27 e Evaluate the UV response 27 e Complete the test record sss ts ale 29 Complete the registration form 31 e Complete the final section of the installation record 30 e Store photocopies of all records and forms in the System Logbook e Store the Installation Guide in the User Manual box AKTAexplorer Installation Guide 18 1139 59 Edition AD 13 4 Installation overview 14 KTAexplorer Installation Guide 18 1139 59 Edition AD Installation of AKTAexplorer 5 5 Installation of AKTAexplorer Begin by creating a clean and dry working area of 200 x 80 cm that allows easy access Then follow the step by step instructions below and fill in the installation record as you go along see page 30 Note Some components are packed in Box 900 located at the top of the system Note Some packing lists are included in the paper boxes 5 1 Unpacking 1 After having removed the cardboard hood check the contents against the attached packing list Check also all included boxes Store all the enclosed paper boxes andplastic bags in a convenient nearby place 2 Release and remove the red strap 1 holding the system to the pallet 3 Lift AKTAexplorer onto the work area using the four strap handles 2 Two people are required to lift the system Do not raise to upright posit2. Connect a syringe to the rinsing tubing that is connected underneath 2 the left pump head on pump A Slowly draw rinsing solution to the syringe When rinsing solution starts to enter thesyringe continue to draw a few millilitres Loosen the syringe and put the tubing in the rinsing solution 1 Purge the pump P 900 as follows Immerse the filters of inlet tubing A11 and B1 in the distilled water Note Never place the reservoir flask below the level of the pump inlet 2 4 22 Connect a male Luer syringe of about 30 ml to the open end of the purge tubing Connect the male Luer connector at the other end of the purge tubing to the left purge valve at pump module A Purge valve Turn the purge valve counter clockwise half a turn to open it and slowly draw eluent into the syringe AKTAexplorer Installation Guide 18 1139 59 Edition AD 5 6 Installation test 6 When fluid starts to enter the syringe continue to draw a few millilitres before closing the purge valve Check that there is novisible air left in the inlet tubing Repeat steps 3 to 5 for all pump heads 6 2 1 Testing pressure stability Perform a pressure test to establish that all air has disappeared from the pump heads Perform as follows 1 Connect a column bypass capillary between the injection valve port 1 and the top of the UV optical unit AKTAexplorer 10 to check pump module A Run 0 2 ml m
3. SampleValve Close C Other Valved xl Help 4 Check that the correct mixer chamber is installed in the mixer 0 6 ml in AKTAexplorer 10 2 0 ml in AKTAexplorer 100 Installation Test Method Guide Buffer A11 Buffer B1 Test flow rate Distilled water 0 4 acetone in distilled water 5 ml min in AKTAexplorer 10 10 ml min in AKTAexplorer 100 Test run time Approximately 30 minutes AKTAexplorer Installation Guide 18 1139 59 Edition AD Installation test 6 6 4 Running the installation test method 1 In UNICORN Main menu select File Printer Setup Select the appropriate printer from the list and select Landscape Then click OK to acknowledge the printer chosen Print Setup Ea Printer Name SEUPPBT_FS1 8L52_D_HP4000_0 Properties Status Ready Type HP LaserJet 4050 Series PCL 5e Where SEUPPBT_FS1 BL52 D _HP4000 Q Comment Paper Orientation Size Ad v Portrait Source auto Select Landscape Network OK Cancel Newa __Canea_ 2 Click the Instant Run button Ee The Instant Run window opens InstantRun E xj For system r Use Wizard Template m Template selection e Method notes Eoc pa column Cancel Help Select the appropriate system and click Run AKTAexplorer Installation Guide 18 1139 59 Edition AD 25 6 26 Installation test 3 Select Installation Test in the Meth
4. 82 4 e Norway Tel 47 815 65 777 Fax 47 815 65 666 Portugal Tel 21 417 7035 Fax 21 417 3184 e Russia amp other C I S amp N I S Tel 7 495 956 5177 Fax 7 495 956 5176 e Spain Tel 902 11 72 65 Fax 935 94 49 65 e Sweden Tel 018 612 1900 Fax 018 612 1910 e Switzerland Tel 0848 8028 10 Fax 0848 8028 11 e UK Tel 0800 515 313 Fax 0800 616 927 e USA Tel 1 800 526 3593 Fax 1 877 295 8102 imagination at work 18 1139 59 AD 01 2007 E Elanders sterv la 2007
5. AKTAexplorer CAUTION The UniNet connection to the computer MUST be made to the board with four LEDs 37 2 If using a fraction collector connect UniNet 1 cable 35 between Pump P 900 and the fraction collector and UniNet 1 cable 36 between the fraction collector and the computer Fraction collector optional 3 All other UniNet 1 cables are connected at delivery A socket WARNING KTAexplorer must be connected to a grounded mains 4 Connect a mains cable 39 supplied between AKTAexplorer and a properly grounded mains socket according to the circled figure Do not switch on Fraction collector optional Wy wtih ii ill To fraction collector To mains socket If using a fraction collector connect a mains cable supplied 38 between the fraction collector and a mains socket at the rear of AKTAexplorer 20 Complete the two first sections of the Installation record on page 30 The installation phase of AKTAexplorer is now completed AKTAexplorer Installation Guide 18 1139 59 Edition AD Installation test 6 6 Installation test The installation test is designed to check the function of the liquid delivery and the UV monitoring system of AKTAexplorer The installation t
6. representative for the most current information a GE Healthcare Bio Sciences AB Bjorkgatan 30 751 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Pacific Tel 85 65 62751830 Fax 85 65 62751829 e Australasia Tel 61 2 8820 8299 Fax 61 2 8820 8200 Austria Tel 01 57606 1613 Fax 01 57606 1614 Belgium Tel 0800 73 890 Fax 02 416 8206 Canada Tel 1 800 463 5800 Fax 1 800 567 1008 Central East amp South East Europe Tel 43 1 972 720 Fax 43 1 972 722 750 e Denmark Tel 45 70 25 24 50 Fax 45 45 16 2424 Eire Tel 1 800 709992 Fax 44 1494 542010 e Finland amp Baltics Tel 358 9 512 3940 Fax 358 9 512 39439 e France Tel 01 69 35 67 00 Fax 01 69 41 98 77 e Germany Tel 0800 9080 711 Fax 0800 9080 712 Greater China Tel 852 2100 6300 Fax 852 2100 6338 e Italy Tel 02 26001 320 Fax 02 26001 399 Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 e Korea Tel 82 2 6201 3700 Fax 82 2 6201 3803 Latin America Tel 55 11 3933 7300 Fax 55 11 3933 7304 e Middle East amp Africa Tel 30 210 96 00 687 Fax 30 210 96 00 693 Netherlands Tel 0800 82 82 82 1 Fax 0800 82 82
7. tested before shipping For safe transportation however some components have been detached and thus need to be remounted Cables capillaries accessories column holder etc are enclosed in paper boxes or in Box 900 located at the top of the instrument pile This guide describes how to install AKTAexplorer The guide is divided into two parts one describing the installation and one describing how to run the installation test After the installation procedure has been performed your AKTAexplorer is ready for purification work For full details of specifications methods maintenance etc refer to the respective User Manuals and Instructions AKTAexplorer Installation Guide 18 1139 59 Edition AD 7 1 About this installation guide 8 AKTAexplorer Installation Guide 18 1139 59 Edition AD Safety 2 2 Safety e The system is designed for indoor use only e Do not use in a dusty atmosphere or close to spraying water Refer to Technical Specifications in the System Manual for detailed environmental pre requisites WARNING The individual instruments must not be opened by the user They contain high voltage circuits that can give a lethal electric shock WARNING Monitor UV 900 uses high intensity ultra violet light Do not disconnect the optical unit while the lamp is ON WARNING AKTAexplorer must be connected to a grounded mains socket WARNING There must always be a sample loop or Superloop c
8. the Curves tab and select the following curves to be displayed Wizard Generated001 1_UV1_265nm 01 SMTH Wizard Generated001 1_UV2_254nm 02 SMTH Wizard Generated001 1_UV3_280nm 03 SMTH Wizard Generated001 1 Conc Click OK Right click in chromatogram window and select Marker Make sure that the UV1 curve value is displayed Read the absorbance for the steps corresponding to Wizard Generated001 1 UV1 265nm 01 SMTH Move the vertical bar to the constant section of each plateau by dragging it Enter theabsorbance values in mAU in column 2 in the Step response table of the Test record see page 29 leaving out the decimals Read the absorbance for the plateaus corresponding to 0 and 100 B for the curves click on the curve name to change the curve reading Wizard Generated001 1 UV1 265nm 01 SMTH Wizard Generated001 1_UV2_254nm 02 SMTH Wizard Generated001 1 UV3_280nm 03 SMTH and enter the values in column 2 in the UV response table of the Test record see page 31 10 Click Print under File to print the chromatogram AKTAexplorer Installation Guide 18 1139 59 Edition AD 29 6 30 Installation test Evaluating the gradient Place a ruler along the gradient part of curve Wizard Generated001 1_UV1_265nm 01 SMTH in the printed report The curve should be linear between 10 B and 90 B and void of discontinuities Evaluating the step response Calculate the relative adsorption plateau heights for curve Wizard Generated001 1_U
9. the pH flow cell holder Note Do not install the pH electrode until the installation test has been performed Note When using the pH electrode the flow restrictor FR 904 must be replaced with the supplied flow restrictor FR 902 Otherwise the long term stability and lifetime of the pH electrode will deteriorate 16 AKTAexplorer Installation Guide 18 1139 59 Edition AD Installation of AKTAexplorer 5 Note For calibration of the pH electrode refer to the UNICORN User Manual 5 3 Installing column holders and tubing holders 1 Install the column holders 17a 17b packed in Box 900 onto the valve door They should be placed close to the column selection valves V2 and V3 Note To release the column holders press the slide clamp 18a 18b downward 2 Install the tubing holder 19 the tube holders 20 and the sample tray 21 by pushing them into the slits below the sample valve V5 AKTAexplorer Installation Guide 18 1139 59 Edition AD 17 5 Installation of AKTAexplorer 3 Locate the sample pump waste tubing 23 penetrating the valve door Remove the wire clips 22 from the tubing and place the outlet in an E flask 24 placed on the sample tray Note For calibration of the sample pump refer to the Pump P 960 User Manual 5 4 Connecting tubings 1 Turn AKTAexplorer on its swivel platform to access the rear and remove
10. GE Healthcare AKTAexplorer Installation Guide AKTA Important user information All users must read this entire manual to fully understand the safe use of AKTAexplorer WARNING The WARNING sign highlights instructions that must be followed to avoid personal injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected se
11. If all values are faulty air in pump or faulty pump e 5 and 95 faulty bad sealing in pumps 5 faulty pump module B 95 faulty pump module A AKTAexplorer Installation Guide 18 1139 59 Edition AD 7 Test record Test record 7 Datel 7 1 Gradient test result Gradient linear from Biala 7 2 Step response test result Step response table 1 2 3 4 5 Programmed Value Baseline cor Normalised Allowed Conc B read rected value value interval 100 95 94 96 70 69 71 30 29 31 5 4 6 0 7 3 UV response test result UV response table 1 2 3 4 5 Wavelength Value read Baseline Absorbance Allowed nm corrected ratio interval 100 B 0 B value 254 265 254 1 11 126 265 265 280 1 26 1 53 280 AKTAexplorer Installation Guide 18 1139 59 Edition AD 31 7 Test record 32 AKTAexplorer Installation Guide 18 1139 59 Edition AD Installation record 8 8 Installation record Check Sign Remarks 1 Unpacking Contents according to packing lists All packing material removed No visible damage Installation Injection valve waste tubings port 4 and 5 marked W1 and W2 to waste reservoir Outlet valve waste tubing port 1 marked W3 extended to waste reservoir Column holder installed pH electrode holder installed optional Computer and printer installed UniNet 1 cabling installed Mains power ca
12. V1_265nm 01 SMTH as follows 1 Subtract the base line value 0 B from each of the values in column 2 in the Step response table of the Test record see page 29 and enter the results in column 3 2 Divide each value in column 3 by the base line corrected value corresponding to 100 B multiply by 100 and enter the results in column 4 The values of column 4 should all fall within the intervals given in column 5 Evaluating the UV response Calculate the UV response ratios in the following way 1 Subtract the base line values 0 B corresponding to each UV curve from the values in column 2 of the UV response table of the Test record see page 29 and enter the results in column 3 2 Calculate the absorbance ratios 265 nm 254 nm and 265 nm 280 nm using the values of column 3 and enter results in column 4 The ratios obtained should all fall within the intervals given in column 5 6 6 Correcting faulty evaluation results Should any of the evaluated values fall outside the specified range proceed as follows e If the system differs from the standard configuration evaluate the result manually If the faulty evaluation result remains continue below 6 6 1 Faulty gradient e The gradient is linear but the interval is too small Mixer chamber too large or faulty mixer e Disturbances may arise from air in the pump pump valves or bad sealings in the pump Refer to the Pump P 900 User Manual 6 6 2 Faulty step response e
13. bling installed 3 Installation test Solutions prepared Tubings to piston seal rinsing system in 20 ethanol AKTAexplorer prepared Installation Test method run Installation Test results evaluated Test Record completed Registration Form completed Test Record and copy of Registration form stored in System Logbook Registration form posted to Service Administration Installation Guide stored in User Manual box for future use AKTAexplorer Installation Guide 18 1139 59 Edition AD 33 8 Installation record 34 AKTAexplorer Installation Guide 18 1139 59 Edition AD Registration form 9 9 Registration form IMPORTANT WARRANTY REGISTRATION INFORMATION Please ensure that this form is completed and returned to Service Administration to register the users reuipment under warranty Name O Ipistitute Companyzazi ia ni ille Le inn noie ahi AGFESS O DEPartMent lOCationiz ss isa ae iii Ai Post COde O Phone NUMber iii Fax NUMDET ERG USE S nn min nn A ESO nina Date of Installation Quote NO me Customer Oder No INVOICE NO Support Agreement purchased with the instrument Y N IfYES give detdllsi iaia aL Installer name 0 SiQ mature OF IA tall n istalla lalial li ziali Note Fill in serial numbers over leaf AKTAexplorer Installation Guide 18 1139 59 Edition AD 35 9 Registration
14. ed the printer automatically prints the chromatogram and the test result Installation Test01 1 LoL Installation Test01 1_UV1_265nm Installation Test01 1 UV2 254nm Installation Test01 1_UV3_280nm av Ta r gt lt gt 6 5 Evaluating the installation test results 6 5 1 Automatic evaluation The system automatically prints the test result when the test is finished The print out consists of a chromatogram and an evaluation of the test result e If the gradient test result is OK the print out says Gradient linearity accepted e If the step response test result is OK the print out says Step response accepted e If the UV response test result is OK the print out says UV response accepted If any of the evaluated values fall outside the specified range go to step 6 6 Correcting faulty evaluation results 28 AKTAexplorer Installation Guide 18 1139 59 Edition AD Installation test 6 6 5 2 Manual evaluation If your chromatography system deviate from the standard configuration e g if optional components have been installed the automatic evaluation will not give a reliable result If so perform a manual evaluation as described below 1 2 Select the UNICORN Main menu module Click on in the Results window and then double click on the Wizard Generated001 icon to open the result file Right click in the Curves pane and select Properties Click
15. est can also be used at any time to check the condition of the system e g after a prolonged stop Correct gradient formation is tested by producing a linear gradient and a series of concentration steps of acetone Correct UV monitoring is tested by monitoring the acetone concentration at 265 254 and 280 nm and calculating the absorbance ratios 265 nm 254 nm and 265 nm 280 nm 6 1 Preparation of AKTAexplorer 6 1 1 Startup of the AKTAexplorer separation unit 1 Switch on the separation unit using the mains switch located to the left on the base platform 6 1 2 Startup of the computer and UNICORN software 1 Switch on the computer the display and the printer according to the instructions in the manufacturer manuals 2 Log into Windows by first pressing Ctrl Alt Del and then clicking OK 3 When the Windows desktop appears start UNICORN by doubleclicking on the UNICORN icon 4 Select user default and enter default as password Click OK AKTAexplorer Installation Guide 18 1139 59 Edition AD 21 6 Installation test 5 Click the System Control button in the taskbar System Control button A Start 4 gt UNICORN Main Menu Method Editor untitled 1 System Control 1 Bjorn lt a Evaluation 6 2 Priming and purging Pump P 900 Prime the piston seal rinsing system of pump P 900 as follows 1 3 1 Immerse the rinsing tubing in a flask 1 containing 20 ethanol in distilled water
16. form 9 1 Components QTY Part Number Description Serial Number System rack Mixer M 925 onitor UV 900 Pump P 900 pH C 900 NV 907 PV 908 SV 903 A SV 903 B Computer Computer display 36 AKTAexplorer Installation Guide 18 1139 59 Edition AD AKTAexplorer Installation Guide 18 1139 59 Edition AD 37 38 AKTAexplorer Installation Guide 18 1139 59 Edition AD AKTAexplorer Installation Guide 18 1139 59 Edition AD 39 www gehealthcare com akta GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden GE imagination at work and GE monogram are trademarks of General Electric Company AKTA AKTAexplorer DropDesign Superloop and UNICORN are trademarks of GE Healthcare companies icrosoft and Windows are either registered trademarks or trademarks of icrosoft Corporation in the United States and or other countries All third party trademarks are the property of their respective owners 2007 General Electric Company All rights reserved GE Healthcare Bio Sciences AB a General Electric Company All goods and services are sold subject to the terms and conditions of sale the company within GE Healthcare which supplies them GE Healthcare reserves the right subject to any regulatory and contractual approval if required to make changes in specifications and features shown herein or discontinue the product described at any time without notice or obligation Contact your local GE Healthcare
17. in at 0 B distilled water Check on the pump display that the pressure reading is stable variation lt 0 02 MPa AKTAexplorer 100 to check pump module A Run 10 ml min at 0 B distilled water Check on the pump display that the pressure reading is stable variation lt 0 2 MPa AKTAexplorer 10 to check pump module 8 Run 0 2 ml min at 100 B distilled water Check on the pump display that the pressure reading is stable variation lt 0 02 MPa AKTAexplorer 100 to check pump module 8 Run 10 ml min at 100 B distilled water Check on the pump display that the pressure reading is stable variation lt 0 2 MPa Proceed to step 5 if the pressure is stable If not consult the Pump P 900 User Manual for troubleshooting instructions Click END AKTAexplorer Installation Guide 18 1139 59 Edition AD 23 6 Installation test 24 6 3 Preparing the installation test 1 Move inlet tubing B1 to a flask containing 500 ml of 0 4 acetone in distilled water 2 Make sure that a bypass capillary is installed between port 1 of the V2 and V3 column selection valves 3 Inthe System Control module select menu Manual Flowpath Then select ColumnPosition Position1Bypass WINCORN_LAB7_NT Flowpath Instructions x Instructions Parameters j oo Injection alve Position __ Insert ColumnPosition R Flowpath OutletValve Position Bypass X Delete BufferValve 1 Alarms amp Mon PumpAlnlet C Frac PumpBlnlet
18. ion yet Lay the system on the same side as on the pallet 4 Release the two red straps with the strap handles Allow the straps to remain taped to the plastic cover 5 Pull back the plastic cover 3 to uncover the swivel platform 4 and raise the system to an upright position 6 Remove the plastic cover with the red straps from the system 7 Save all the original packing material If for any reason the equipment has to be repacked for transportation or otherwise it is important that the system can be safely packed using the original packing material AKTAexplorer Installation Guide 18 1139 59 Edition AD 15 5 Installation of AKTAexplorer 5 2 Installing the pH flow cell 1 Install the pH flow cell holder packed in Box 900 in the groove directly below the outlet valve V4 on the outside of the valve door 2 Push the slide clamp upwards to secure the pH flow cell holder 3 Connect capillary G12 to the inlet of the pH flow cell 4 Connect the outlet of the pH flow cell to the FR 904 flow restrictor marked IN using the G13 capillary 5 Connect the outlet of the FR 904 flow restrictor to the centre port of the V4 outlet valve using the G14 capillary 6 Place the FR 904 flow restrictor 14 in the hole in the pH flow cell holder 7 Mount the pH electrode 16 according to the figure for experiments where pH is to be measured Otherwise mount the pH electrode dummy in the hole in
19. od Wizard Click Run Method Wizard for System Explorer 100F gt Main Selection Column and BufferPrep Mai lt Back Run Cancel Help Set Default 4 Inthe Evaluation Procedures window select the procedure for your system for example InstTest_Explorer100 Evaluation Procedures xl Selected E valuation Procedures will run at the end of the method Integrate_and_Print _JPrint_Chromatogram IC Integrate BP Report IC BP Report Chromatogram MinstT est_Explorer100 lnstTest_Basic100 InstTest_Purifier1 00 ID Chromatofocusing Note Explorer 10 systems have a specific evaluation procedure that includes the autosampler peaks The name of the procedure has the extension _A90X 5 Click Next in the Method Information window 6 Click START in the Result Name window to start the installation test The progress of the test is monitored in the System Control module The installation test run time is approximately 30 min 7 To customize the Curves pane right click in the pane and select Properties AKTAexplorer Installation Guide 18 1139 59 Edition AD Installation test 6 8 Click the Curves tab Properties 9 Select the following curves to be displayed e UV1_265nm e UV2_254nm e UV3_280nm e Conc Clear all other highlighted curves Click OK AKTAexplorer Installation Guide 18 1139 59 Edition AD 27 6 Installation test 10 When the test is finish
20. onnected to ports 2 and 6 of the injection valve This prevents liquid spraying out of the ports when switching the valve which is especially dangerous if hazardous chemicals are being used WARNING Two people are required to lift the system WARNING Never block the ports on the outlet valve with stop plugs since this will create over pressure and might result in injury WARNING Only spare parts approved or supplied by GE Healthcare may be used for maintaining and servicing the system WARNING If the system is turned around or the fraction collector removed the external capillaries and other tubings may become entangled in nearby objects and be pulled from their connections causing leakage WARNING Never place waste containers on the top of the system If they become full and overflow liquid may penetrate the system causing a short circuit WARNING If heavier instrument components such as the pump are removed from the rack and the door is then opened fully the shift in the centre of gravity of the system may cause it to tip over PP Peer PRRP AKTAexplorer Installation Guide 18 1139 59 Edition AD 9 2 Safety 10 A A WARNING Never place buffer containers on the top of the valve door f this is done the containers may fall down when the valve door is opened Place the buffer containers on the buffer tray above Box 900 WARNING If the door is quickly pulled
21. open to its full extent the internal capillary tubings may be pulled from their connections causing leakage AKTAexplorer Installation Guide 18 1139 59 Edition AD Pre requisites 3 3 Pre requisites WARNING AKTAexplorer must be connected to a grounded mains l socket e Two people are required to lift AKTAexplorer onto the working bench e To install AKTAexplorer a working area of about 200 x 80 cm is required e AKTAexplorer requires 100 120 220 240 V 50 60 Hz electrical supply with safety grounding e Cutting pliers are recommended for cutting plastic straps e Awaste flask is needed e The installation test requires the following solutions 1000 ml of distilled water for priming and purging the pump 500 ml of 0 4 acetone in distilled water 100 ml of 20 ethanol in distilled water AKTAexplorer Installation Guide 18 1139 59 Edition AD 11 3 Pre requisites 12 AKTAexplorer Installation Guide 18 1139 59 Edition AD Installation overview 4 4 Installation overview m Unpack AKTAGX plOner nn E T 12 e Detach packing material and install items enclosed 12 e Unpack and install the computer 16 Connect mains power CODING 17 e Connect UniNet 1 data communication chain cabling 16 e Complete the first two sections of the installation record 30 e Prepare AKTAexplorer for the installation test
22. parately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment AKTAexplorer Installation Guide 18 1139 59 Edition AD Contents Contents 1 About this installation guide 2 Safety 3 Pre requisites 4 Installation overview 5 Installation of AKTAexplorer 5 1 Unpacking 15 5 2 Installing the pH flow cell 16 5 3 Installing column holders and tubing holders 17 5 4 Connecting tubings 18 5 5 Installing the computer and the mains cable 19 6 Installation test 6 1 Preparation of AKTAexplorer 21 6 1 1 Startup of the AKTAexplorer separation unit 21 6 1 2 Startup of the computer and UNICORN software 21 6 2 Priming and purging Pump P 900 22 6 2 1 Testing pressure stability 23 6 3 Preparing the installation test 24 6 4 Running the installation test method 25 6 5 Evaluating the installation test results 28 6 5 1 Automatic evaluation 28 6 5 2 Manual evaluation 29 6 6 Correcting faulty evaluation results 30 6 6 1 Faulty gradient 30 6 6 2 Faulty step response 30 7 Test record 7 1 Gradient test result 31 7 2 Step response test result 31 7 3 UV response test result 31 8 Installation record 9 Registration form 9 1 Components 36 AKTAexplorer Installation Guide 18 1139 59 Edition AD 5 Contents 6 AKTAexplorer Installation Guide 18 1139 59 Edition AD About this installation guide 1 1 About this installation guide AkTAexplorer is assembled and fully
23. the plastic bag from the inlet tubings marked A11 A2 B1 and B2 2 Locate the buffer valve V6 and connect the tubing marked A1 25 to the central port of the buffer valve 18 AKTAexplorer Installation Guide 18 1139 59 Edition AD Installation of AKTAexplorer 5 3 Snap the inlet tubings A11 A2 B1 and B2 into the tubing holder 26 located at the upper left 4 Turn AKTAexplorer on its swivel platform to access the valve door 5 Remove the wire clips from the waste tubing connected to port 5 29 on the injection valve V1 6 Place the waste tubing in a waste bottle and place the bottle in front of AKTAexplorer or where convenient Note The installation of a fraction collector optional is described in detail in the AKTAexplorer Optional Configurations User Manual Refer to the section that describes your fraction collector 5 5 Installing the computer and the mains cable Unpack and install the computer and printer according to the manufacturer s instructions Place them to the left of the system Do not switch them on CAUTION The mains power to AKTAexplorer must be switched OFF before the UniNet 1 cabling is installed 1 Complete the UniNet 1 data communication chain by connecting a UniNet 1 cable between the computer and Pump P 900 AKTAexplorer Installation Guide 18 1139 59 Edition AD 19 5 Installation of
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